US20040162420A1 - Cloning of cytochrome p450 genes from nicotiana - Google Patents

Cloning of cytochrome p450 genes from nicotiana Download PDF

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US20040162420A1
US20040162420A1 US10/686,947 US68694703A US2004162420A1 US 20040162420 A1 US20040162420 A1 US 20040162420A1 US 68694703 A US68694703 A US 68694703A US 2004162420 A1 US2004162420 A1 US 2004162420A1
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US10/686,947
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Dongmei Xu
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US Smokeless Tobacco Co LLC
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US Smokeless Tobacco Co LLC
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Priority claimed from US10/397,346 external-priority patent/US6761492B2/en
Application filed by US Smokeless Tobacco Co LLC filed Critical US Smokeless Tobacco Co LLC
Priority to US10/686,947 priority Critical patent/US20040162420A1/en
Publication of US20040162420A1 publication Critical patent/US20040162420A1/en
Priority to US10/934,944 priority patent/US7812227B2/en
Priority to US10/943,507 priority patent/US7855318B2/en
Priority to AT04795391T priority patent/ATE554161T1/en
Priority to CN2004800303850A priority patent/CN1867674B/en
Priority to PL04795252T priority patent/PL1675957T3/en
Priority to PL04795391T priority patent/PL1694832T3/en
Priority to AU2004282588A priority patent/AU2004282588B2/en
Priority to BRPI0415363-4A priority patent/BRPI0415363A/en
Priority to CN200480030387XA priority patent/CN1926235B/en
Priority to AU2004282576A priority patent/AU2004282576B2/en
Priority to ZA200602938A priority patent/ZA200602938B/en
Priority to EP10012983A priority patent/EP2330191A1/en
Priority to ES04795252T priority patent/ES2377868T3/en
Priority to JP2006535343A priority patent/JP5422099B2/en
Priority to CN2012101717953A priority patent/CN102703477A/en
Priority to EP04795252A priority patent/EP1675957B1/en
Priority to CN2011102770337A priority patent/CN102321641A/en
Priority to PCT/US2004/034218 priority patent/WO2005038018A2/en
Priority to AT04795252T priority patent/ATE536415T1/en
Priority to EP04795391A priority patent/EP1694832B1/en
Priority to JP2006535378A priority patent/JP4519137B2/en
Priority to ES04795391T priority patent/ES2384243T3/en
Priority to PCT/US2004/034065 priority patent/WO2005038033A2/en
Priority to BRPI0415362-6A priority patent/BRPI0415362A/en
Priority to US11/110,062 priority patent/US7700851B2/en
Priority to US11/116,881 priority patent/US7700834B2/en
Assigned to U.S. SMOKELESS TOBACCO COMPANY reassignment U.S. SMOKELESS TOBACCO COMPANY ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: XU, DONGMEI
Priority to US11/694,507 priority patent/US20070292871A1/en
Priority to HK07109796.0A priority patent/HK1104575A1/en
Priority to JP2009276020A priority patent/JP5558797B2/en
Priority to US12/755,733 priority patent/US8581043B2/en
Priority to US12/760,905 priority patent/US8592663B2/en
Priority to US12/762,613 priority patent/US8637731B2/en
Priority to AU2010201696A priority patent/AU2010201696B2/en
Priority to AU2010201695A priority patent/AU2010201695B2/en
Priority to US12/821,273 priority patent/US8058504B2/en
Priority to JP2010166914A priority patent/JP5721357B2/en
Priority to US12/901,878 priority patent/US8436235B2/en
Priority to US13/243,270 priority patent/US8188337B2/en
Priority to US13/481,297 priority patent/US8658856B2/en
Priority to US13/887,913 priority patent/US9464297B2/en
Priority to US14/087,204 priority patent/US9322030B2/en
Priority to US15/136,624 priority patent/US10266836B2/en
Priority to US16/289,210 priority patent/US10954526B2/en
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

Definitions

  • the present invention relates to nucleic acid sequences encoding cytochrome p450 enzymes (hereinafter referred to as p450 and p450 enzymes) in Nicotiana plants and methods for using those nucleic acid sequences to alter plant phenotypes.
  • p450 and p450 enzymes cytochrome p450 enzymes
  • Cytochrome p450s catalyze enzymatic reactions for a diverse range of chemically dissimilar substrates that include the oxidative, peroxidative and reductive metabolism of endogenous and xenobiotic substrates.
  • p450s participate in biochemical pathways that include the synthesis of plant products such as phenylpropanoids, alkaloids, terpenoids, lipids, cyanogenic glycosides, and glucosinolates (Chappel, Annu. Rev. Plant Physiol. Plant Mol. Biol. 198, 49:311-343).
  • Cytochrome p450s also known as p450 heme-thiolate proteins, usually act as terminal oxidases in multi-component electron transfer chains, called p450-containing monooxygenase systems. Specific reactions catalyzed include demethylation, hydroxylation, epoxidation, N-oxidation, sulfooxidation, N-, S-, and O-dealkylations, desulfation, deamination, and reduction of azo, nitro, and N-oxide groups.
  • Nicotiana plant p450 enzymes have been implicated in effecting a variety of plant metabolites such as phenylpropanoids, alkaloids, terpenoids, lipids, cyanogenic glycosides, glucosinolates and a host of other chemical entities.
  • plant metabolites such as phenylpropanoids, alkaloids, terpenoids, lipids, cyanogenic glycosides, glucosinolates and a host of other chemical entities.
  • some p450 enzymes can impact the composition of plant metabolites in plants. For example, it has been long desired to improve the flavor and aroma of certain plants by altering its profile of selected fatty acids through breeding; however very little is known about mechanisms involved in controlling the levels of these leaf constituents.
  • the down regulation of p450 enzymes associated with the modification of fatty acids may facilitate accumulation of desired fatty acids that provide more preferred leaf phenotypic qualities.
  • p450 enzymes The function of p450 enzymes and their broadening roles in plant constituents is still being discovered. For instance, a special class of p450 enzymes was found to catalyze the breakdown of fatty acid into volatile C6- and C9-aldehydes and -alcohols that are major contributors of “fresh green” odor of fruits and vegetables.
  • the level of other novel targeted p450s may be altered to enhance the qualities of leaf constituents by modifying lipid composition and related break down metabolites in Nicotiana leaf. Several of these constituents in leaf are affected by senescence that stimulates the maturation of leaf quality properties. Still other reports have shown that p450s enzymes are play a functional role in altering fatty acids that are involved in plant-pathogen interactions and disease resistance.
  • p450 enzymes have been suggested to be involved in alkaloid biosynthesis.
  • Nornicotine is a minor alkaloid found in Nicotiana tabaceum. It has been postulated that it is produced by the p450 mediated demethylation of nicotine followed by acylation and nitrosation at the N position thereby producing a series of N-acylnonicotines and N-nitrosonornicotines.
  • N-demethylation catalyzed by a putative p450 demethylase, is thought to be a primary source of nornicotine biosyntheses in Nicotiana. While the enzyme is believed to be microsomal, thus far a nicotine demethylase enzyme has not been successfully purified, nor have the genes involved been isolated.
  • the present invention is directed to plant p450 enzymes.
  • the present invention is further directed to plant p450 enzymes from Nicotiana.
  • the present invention is also directed to p450 enzymes in plants whose expression is induced by ethylene and/or plant senescence.
  • the present invention is yet further directed to nucleic acid sequences in plants having enzymatic activities, for example, being categorized as oxygenase, demethylase and the like, or other and the use of those sequences to reduce or silence the expression or over-expression of these enzymes.
  • the invention also relates to p450 enzymes found in plants containing higher nornicotine levels than plants exhibiting lower nornicotine levels.
  • the invention is directed to nucleic acid sequences as set forth in SEQ. ID. Nos. 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191,
  • the invention is directed to amino acid sequences as set forth in SEQ. ID. Nos. 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194,
  • the invention is directed to amino acid sequences of full length genes as set forth in SEQ. ID. Nos. 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292, 294, 296 and 298.
  • the invention is directed to amino acid sequences of the fragments set forth in SEQ. ID. Nos. 299-357.
  • those fragments containing 90% or greater identity in amino acid sequence were placed into groups dependent upon their identity to each other in a region corresponding to the first cytochrome p450 domain, UXXRXXZ, to the third cytochrome domain, GXRXO, where U is E or K, X is any amino acid and Z is R, T, S or M.
  • the representative amino acid groups respective species shown in Table IV.
  • the reduction or elimination or over-expression of p450 enzymes in Nicotiana plants may be accomplished transiently using RNA viral systems.
  • Resulting transformed or infected plants are assessed for phenotypic changes including, but not limited to, analysis of endogenous p450 RNA transcripts, p450 expressed peptides, and concentrations of plant metabolites using techniques commonly available to one having ordinary skill in the art.
  • the present invention is also directed to generation of trangenic Nicotiana lines that have altered p450 enzyme activity levels.
  • these transgenic lines include nucleic acid sequences that are effective for reducing or silencing or increasing the expression of certain enzyme thus resulting in phenotypic effects within Nicotiana.
  • nucleic acid sequences include SEQ. ID. Nos.
  • plant cultivars including nucleic acids of the present invention in a down regulation capacity using either full length genes or fragments thereof or in an over-expression capacity using full length genes will have altered metabolite profiles relative to control plants.
  • plant cultivars including nucleic acid of the present invention using either full length genes or fragments thereof in modifying the biosynthesis or breakdown of metabolites derived from the plant or external to the plants will have use in tolerating certain exogenous chemicals or plant pests.
  • nucleic acid sequences include SEQ ID. Nos.
  • the present invention is directed to the screening of plants, more preferably Nicotiana, that contain genes that have substantial nucleic acid identity to the taught nucleic acid sequence.
  • the use of the invention would be advantageous to identify and select plants that contain a nucleic acid sequence with exact or substantial identity where such plants are part of a breeding program for traditional or transgenic varieties, a mutagenesis program, or naturally occurring diverse plant populations.
  • the screening of plants for substantial nucleic acid identity may be accomplished by evaluating plant nucleic acid materials using a nucleic acid probe in conjunction with nucleic acid detection protocols including, but not limited to, nucleic acid hybridization and PCR analysis.
  • the nucleic acid probe may consist of the taught nucleic acid sequence or fragment thereof corresponding to SEQ ID 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195,
  • the present invention is directed to the identification of plant genes, more preferably Nicotiana, that share substantial amino acid identity corresponding to the taught nucleic acid sequence.
  • the identification of plant genes including both cDNA and genomic clones, those cDNAs and genomic clones, more preferably from Nicotiana may be accomplished by screening plant cDNA libraries using a nucleic acid probe in conjunction with nucleic acid detection protocols including, but not limited to, nucleic acid hybridization and PCR analysis.
  • the nucleic acid probe may be comprised of nucleic acid sequence or fragment thereof corresponding to SEQ ID 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 143, 145 and 147.
  • cDNA expression libraries that express peptides may be screened using antibodies directed to part or all of the taught amino acid sequence.
  • amino acid sequences include SEQ ID 2, 4, 8, 9, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 144, 146, 148.
  • the present invention is also directed to generation of transgenic Nicotiana lines that have over-expression of p450 enzyme activity levels.
  • these transgenic lines include all nucleic acid sequences encoding the amino acid sequences of full length genes that are effective for increasing the expression of certain enzyme thus resulting in phenotypic effects within Nicotiana.
  • amino acid sequences include SEQ. ID.
  • FIG. 1 shows nucleic acid SEQ. ID. No.:1 and amino acid SEQ. ID. No.:2.
  • FIG. 2 shows nucleic acid SEQ. ID. No.:3 and amino acid SEQ. ID. No.:4.
  • FIG. 3 shows nucleic acid SEQ. ID. No.:5 and amino acid SEQ. ID. No.:6.
  • FIG. 4 shows nucleic acid SEQ. ID. No.:7 and amino acid SEQ. ID. No.:8.
  • FIG. 5 shows nucleic acid SEQ. ID. No.:9 and amino acid SEQ. ID. No.:10.
  • FIG. 6 shows nucleic acid SEQ. ID. No.:11 and amino acid SEQ. ID. No.:12.
  • FIG. 7 shows nucleic acid SEQ. ID. No.:13 and amino acid SEQ. ID. No.:14.
  • FIG. 8 shows nucleic acid SEQ. ID. No.:15 and amino acid SEQ. ID. No.:16.
  • FIG. 9 shows nucleic acid SEQ. ID. No.:17 and amino acid SEQ. ID. No.:18.
  • FIG. 10 shows nucleic acid SEQ. ID. No.:19 and amino acid SEQ. ID. No.:20.
  • FIG. 11 shows nucleic acid SEQ. ID. No.:21 and amino acid SEQ. ID. No.:22.
  • FIG. 12 shows nucleic acid SEQ. ID. No.:23 and amino acid SEQ. ID. No.:24.
  • FIG. 13 shows nucleic acid SEQ. ID. No.:25 and amino acid SEQ. ID. No.:26.
  • FIG. 14 shows nucleic acid SEQ. ID. No.:27 and amino acid SEQ. ID. No.:28.
  • FIG. 15 shows nucleic acid SEQ. ID. No.:29 and amino acid SEQ. ID. No.:30.
  • FIG. 16 shows nucleic acid SEQ. ID. No.:31 and amino acid SEQ. ID. No.:32.
  • FIG. 17 shows nucleic acid SEQ. ID. No.:33 and amino acid SEQ. ID. No.:34.
  • FIG. 18 shows nucleic acid SEQ. ID. No.:35 and amino acid SEQ. ID. No.:36.
  • FIG. 19 shows nucleic acid SEQ. ID. No.:37 and amino acid SEQ. ID. No.:38.
  • FIG. 20 shows nucleic acid SEQ. ID. No.:39 and amino acid SEQ. ID. No.:40.
  • FIG. 21 shows nucleic acid SEQ. ID. No.:41 and amino acid SEQ. ID. No.:42.
  • FIG. 22 shows nucleic acid SEQ. ID. No.:43 and amino acid SEQ. ID. No.:44.
  • FIG. 23 shows nucleic acid SEQ. ID. No.:45 and amino acid SEQ. ID. No.:46.
  • FIG. 24 shows nucleic acid SEQ. ID. No.:47 and amino acid SEQ. ID. No.:48.
  • FIG. 25 shows nucleic acid SEQ. ID. No.:49 and amino acid SEQ. ID. No.:50.
  • FIG. 26 shows nucleic acid SEQ. ID. No.:51 and amino acid SEQ. ID. No.:52.
  • FIG. 27 shows nucleic acid SEQ. ID. No.:53 and amino acid SEQ. ID. No.:54.
  • FIG. 28 shows nucleic acid SEQ. ID. No.:55 and amino acid SEQ. ID. No.:56.
  • FIG. 29 shows nucleic acid SEQ. ID. No.:57 and amino acid SEQ. ID. No.:58.
  • FIG. 30 shows nucleic acid SEQ. ID. No.:59 and amino acid SEQ. ID. No.:60.
  • FIG. 31 shows nucleic acid SEQ. ID. No.:61 and amino acid SEQ. ID. No.:62.
  • FIG. 32 shows nucleic acid SEQ. ID. No.:63 and amino acid SEQ. ID. No.:64.
  • FIG. 33 shows nucleic acid SEQ. ID. No.:65 and amino acid SEQ. ID. No.:66.
  • FIG. 34 shows nucleic acid SEQ. ID. No.:67 and amino acid SEQ. ID. No.:68.
  • FIG. 35 shows nucleic acid SEQ. ID. No.:69 and amino acid SEQ. ID. No.:70.
  • FIG. 36 shows nucleic acid SEQ. ID. No.:71 and amino acid SEQ. ID. No.:72.
  • FIG. 37 shows nucleic acid SEQ. ID. No.:73 and amino acid SEQ. ID. No.:74.
  • FIG. 38 shows nucleic acid SEQ. ID. No.:75 and amino acid SEQ. ID. No.:76.
  • FIG. 39 shows nucleic acid SEQ. ID. No.:77 and amino acid SEQ. ID. No.:78.
  • FIG. 40 shows nucleic acid SEQ. ID. No.:79 and amino acid SEQ. ID. No.:80.
  • FIG. 41 shows nucleic acid SEQ. ID. No.:81 and amino acid SEQ. ID. No.:82.
  • FIG. 42 shows nucleic acid SEQ. ID. No.:83 and amino acid SEQ. ID. No.:84.
  • FIG. 43 shows nucleic acid SEQ. ID. No.:85 and amino acid SEQ. ID. No.:86.
  • FIG. 44 shows nucleic acid SEQ. ID. No.:87 and amino acid SEQ. ID. No.:88.
  • FIG. 45 shows nucleic acid SEQ. ID. No.:89 and amino acid SEQ. ID. No.:90.
  • FIG. 46 shows nucleic acid SEQ. ID. No.:91 and amino acid SEQ. ID. No.:92.
  • FIG. 48 shows nucleic acid SEQ. ID. No.:95 and amino acid SEQ. ID. No.:96.
  • FIG. 49 shows nucleic acid SEQ. ID. No.:97 and amino acid SEQ. ID. No.:98.
  • FIG. 50 shows nucleic acid SEQ. ID. No.:99 and amino acid SEQ. ID. No.:100.
  • FIG. 51 shows nucleic acid SEQ. ID. No.:101 and amino acid SEQ. ID. No.:102.
  • FIG. 52 shows nucleic acid SEQ. ID. No.:103 and amino acid SEQ. ID. No.:104.
  • FIG. 53 shows nucleic acid SEQ. ID. No.:105 and amino acid SEQ. ID. No.:106.
  • FIG. 54 shows nucleic acid SEQ. ID. No.:107 and amino acid SEQ. ID. No.:108.
  • FIG. 55 shows nucleic acid SEQ. ID. No.:109 and amino acid SEQ. ID. No.:110.
  • FIG. 56 shows nucleic acid SEQ. ID. No.:111 and amino acid SEQ. ID. No.:112.
  • FIG. 57 shows nucleic acid SEQ. ID. No.:113 and amino acid SEQ. ID. No.:114.
  • FIG. 58 shows nucleic acid SEQ. ID. No.:115 and amino acid SEQ. ID. No.:116.
  • FIG. 59 shows nucleic acid SEQ. ID. No.:117 and amino acid SEQ. ID. No.:118.
  • FIG. 60 shows nucleic acid SEQ. ID. No.:119 and amino acid SEQ. ID. No.:120.
  • FIG. 61 shows nucleic acid SEQ. ID. No.:121 and amino acid SEQ. ID. No.:122.
  • FIG. 62 shows nucleic acid SEQ. ID. No.:123 and amino acid SEQ. ID. No.:124.
  • FIG. 63 shows nucleic acid SEQ. ID. No.:125 and amino acid SEQ. ID. No.:126.
  • FIG. 64 shows nucleic acid SEQ. ID. No.:127 and amino acid SEQ. ID. No.:128.
  • FIG. 65 shows nucleic acid SEQ. ID. No.:129 and amino acid SEQ. ID. No.:130.
  • FIG. 66 shows nucleic acid SEQ. ID. No.:131 and amino acid SEQ. ID. No.:132.
  • FIG. 67 shows nucleic acid SEQ. ID. No.:133 and amino acid SEQ. ID. No.:134.
  • FIG. 68 shows nucleic acid SEQ. ID. No.:135 and amino acid SEQ. ID. No.:136.
  • FIG. 69 shows nucleic acid SEQ. ID. No.:137 and amino acid SEQ. ID. No.:138.
  • FIG. 70 shows nucleic acid SEQ. ID. No.:139 and amino acid SEQ. ID. No.:140.
  • FIG. 72 shows nucleic acid SEQ. ID. No.:143 and amino acid SEQ. ID. No.:144.
  • FIG. 73 shows nucleic acid SEQ. ID. No.:145 and amino acid SEQ. ID. No.:146.
  • FIG. 74 shows nucleic acid SEQ. ID. No.:147 and amino acid SEQ. ID. No.:148.
  • FIG. 75 shows nucleic acid SEQ. ID No.: 149 and amino acid SEQ. ID. No.: 150.
  • FIG. 76 shows nucleic acid SEQ. ID No.: 151 and amino acid SEQ. ID. No.: 152.
  • FIG. 77 shows nucleic acid SEQ. ID No.: 153 and amino acid SEQ. ID. No.: 154.
  • FIG. 78 shows nucleic acid SEQ. ID No.: 155 and amino acid SEQ. ID. No.: 156.
  • FIG. 79 shows nucleic acid SEQ. ID No.: 157 and amino acid SEQ. ID. No.: 158.
  • FIG. 80 shows nucleic acid SEQ. ID No.: 159 and amino acid SEQ. ID. No.: 160.
  • FIG. 81 shows nucleic acid SEQ. ID No.: 161 and amino acid SEQ. ID. No.: 162.
  • FIG. 82 shows nucleic acid SEQ. ID No.: 163 and amino acid SEQ. ID. No.: 164.
  • FIG. 83 shows nucleic acid SEQ. ID No.: 165 and amino acid SEQ. ID. No.: 166.
  • FIG. 84 shows nucleic acid SEQ. ID No.: 167 and amino acid SEQ. ID. No.: 168.
  • FIG. 85 shows nucleic acid SEQ. ID No.: 169 and amino acid SEQ. ID. No.: 170.
  • FIG. 86 shows nucleic acid SEQ. ID No.: 171 and amino acid SEQ. ID. No.: 172.
  • FIG. 87 shows nucleic acid SEQ. ID No.: 173 and amino acid SEQ. ID. No.: 174.
  • FIG. 88 shows nucleic acid SEQ. ID No.: 175 and amino acid SEQ. ID. No.: 176.
  • FIG. 89 shows nucleic acid SEQ. ID No.: 177 and amino acid SEQ. ID. No.: 178.
  • FIG. 90 shows nucleic acid SEQ. ID No.: 179 and amino acid SEQ. ID. No.: 180.
  • FIG. 91 shows nucleic acid SEQ. ID No.: 181 and amino acid SEQ. ID. No.: 182.
  • FIG. 92 shows nucleic acid SEQ. ID No.: 183 and amino acid SEQ. ID. No.: 184.
  • FIG. 93 shows nucleic acid SEQ. ID No.: 185 and amino acid SEQ. ID. No.: 186.
  • FIG. 94 shows nucleic acid SEQ. ID No.: 187 and amino acid SEQ. ID. No.: 188.
  • FIG. 95 shows nucleic acid SEQ. ID No.: 189 and amino acid SEQ. ID. No.: 190.
  • FIG. 96 shows nucleic acid SEQ. ID No.: 191 and amino acid SEQ. ID. No.: 192.
  • FIG. 97 shows nucleic acid SEQ. ID No.: 193 and amino acid SEQ. ID. No.: 194.
  • FIG. 98 shows nucleic acid SEQ. ID No.: 195 and amino acid SEQ. ID. No.: 196.
  • FIG. 99 shows nucleic acid SEQ. ID No.: 197 and amino acid SEQ. ID. No.: 198.
  • FIG. 100 shows nucleic acid SEQ. ID No.: 199 and amino acid SEQ. ID. No.: 200.
  • FIG. 101 shows nucleic acid SEQ. ID No.: 201 and amino acid SEQ. ID. No.: 202.
  • FIG. 102 shows nucleic acid SEQ. ID No.: 203 and amino acid SEQ. ID. No.: 204.
  • FIG. 103 shows nucleic acid SEQ. ID No.: 205 and amino acid SEQ. ID. No.: 206.
  • FIG. 104 shows nucleic acid SEQ. ID No.: 207 and amino acid SEQ. ID. No.: 208.
  • FIG. 105 shows nucleic acid SEQ. ID No.: 209 and amino acid SEQ. ID. No.: 210.
  • FIG. 106 shows nucleic acid SEQ. ID No.: 211 and amino acid SEQ. ID. No.: 212.
  • FIG. 107 shows nucleic acid SEQ. ID No.: 213 and amino acid SEQ. ID. No.: 214.
  • FIG. 108 shows nucleic acid SEQ. ID No.: 215 and amino acid SEQ. ID. No.: 216.
  • FIG. 109 shows nucleic acid SEQ. ID No.: 217 and amino acid SEQ. ID. No.: 218.
  • FIG. 110 shows nucleic acid SEQ. ID No.: 219 and amino acid SEQ. ID. No.: 220.
  • FIG. 111 shows nucleic acid SEQ. ID No.: 221 and amino acid SEQ. ID. No.: 222.
  • FIG. 112 shows nucleic acid SEQ. ID No.: 223 and amino acid SEQ. ID. No.: 224.
  • FIG. 113 shows nucleic acid SEQ. ID No.: 225 and amino acid SEQ. ID. No.: 226.
  • FIG. 114 shows nucleic acid SEQ. ID No.: 227 and amino acid SEQ. ID. No.: 228.
  • FIG. 115 shows nucleic acid SEQ. ID No.: 229 and amino acid SEQ. ID. No.: 230.
  • FIG. 116 shows nucleic acid SEQ. ID No.: 231 and amino acid SEQ. ID. No.: 232.
  • FIG. 117 shows nucleic acid SEQ. ID No.: 233 and amino acid SEQ. ID. No.: 234.
  • FIG. 118 shows nucleic acid SEQ. ID No.: 235 and amino acid SEQ. ID. No.: 236.
  • FIG. 119 shows nucleic acid SEQ. ID No.: 237 and amino acid SEQ. ID. No.: 238.
  • FIG. 120 shows nucleic acid SEQ. ID No.: 239 and amino acid SEQ. ID. No.: 240.
  • FIG. 121 shows nucleic acid SEQ. ID No.: 241 and amino acid SEQ. ID. No.: 242.
  • FIG. 122 shows nucleic acid SEQ. ID No.: 243 and amino acid SEQ. ID. No.: 244.
  • FIG. 123 shows nucleic acid SEQ. ID No.: 245 and amino acid SEQ. ID. No.: 246.
  • FIG. 124 shows nucleic acid SEQ. ID No.: 247 and amino acid SEQ. ID. No.: 248.
  • FIG. 125 shows nucleic acid SEQ. ID No.: 249 and amino acid SEQ. ID. No.: 250.
  • FIG. 126 shows nucleic acid SEQ. ID No.: 251 and amino acid SEQ. ID. No.: 252.
  • FIG. 127 shows nucleic acid SEQ. ID No.: 253 and amino acid SEQ. ID. No.: 254.
  • FIG. 128 shows nucleic acid SEQ. ID No.: 255 and amino acid SEQ. ID. No.: 256.
  • FIG. 129 shows nucleic acid SEQ. ID No.: 257 and amino acid SEQ. ID. No.: 258.
  • FIG. 130 shows nucleic acid SEQ. ID No.: 259 and amino acid SEQ. ID. No.: 260.
  • FIG. 131 shows nucleic acid SEQ. ID No.: 261 and amino acid SEQ. ID. No.: 262.
  • FIG. 132 shows nucleic acid SEQ. ID No.: 263 and amino acid SEQ. ID. No.: 264.
  • FIG. 133 shows nucleic acid SEQ. ID No.: 265 and amino acid SEQ. ID. No.: 266.
  • FIG. 134 shows nucleic acid SEQ. ID No.: 267 and amino acid SEQ. ID. No.: 268.
  • FIG. 135 shows nucleic acid SEQ. ID No.: 269 and amino acid SEQ. ID. No.: 270.
  • FIG. 136 shows nucleic acid SEQ. ID No.: 271 and amino acid SEQ. ID. No.: 272.
  • FIG. 137 shows nucleic acid SEQ. ID No.: 273 and amino acid SEQ. ID. No.: 274.
  • FIG. 138 shows nucleic acid SEQ. ID No.: 275 and amino acid SEQ. ID. No.: 276.
  • FIG. 139 shows nucleic acid SEQ. ID No.: 277 and amino acid SEQ. ID. No.: 278.
  • FIG. 140 shows nucleic acid SEQ. ID No.: 279 and amino acid SEQ. ID. No.: 280.
  • FIG. 141 shows nucleic acid SEQ. ID No.: 281 and amino acid SEQ. ID. No.: 282.
  • FIG. 142 shows nucleic acid SEQ. ID No.: 283 and amino acid SEQ. ID. No.: 284.
  • FIG. 143 shows nucleic acid SEQ. ID No.: 285 and amino acid SEQ. ID. No.: 286.
  • FIG. 144 shows nucleic acid SEQ. ID No.: 287 and amino acid SEQ. ID. No.: 288.
  • FIG. 145 shows nucleic acid SEQ. ID No.: 289 and amino acid SEQ. ID. No.: 290.
  • FIG. 146 shows nucleic acid SEQ. ID No.: 291 and amino acid SEQ. ID. No.: 292.
  • FIG. 147 shows nucleic acid SEQ. ID No.: 293 and amino acid SEQ. ID. No.: 294.
  • FIG. 148 shows nucleic acid SEQ. ID No.: 295 and amino acid SEQ. ID. No.: 296.
  • FIG. 149 shows nucleic acid SEQ. ID No.: 297 and amino acid SEQ. ID. No.: 298.
  • FIG. 151 shows a comparison of Sequence Groups.
  • FIG. 152 illustrates alignment of full length clones.
  • FIG. 153 shows a procedure used for cloning of cytochrome p450 cDNA fragments by PCR
  • nucleic acid refers to a deoxyribonucleotide or ribonucleotide polymer in either single- or double-stranded form, or sense or anti-sense, and unless otherwise limited, encompasses known analogues of natural nucleotides that hybridize to nucleic acids in a manner similar to naturally occurring nucleotides. Unless otherwise indicated, a particular nucleic acid sequence includes the complementary sequence thereof.
  • operably linked refers to functional linkage between a nucleic acid expression control sequence (such as a promoter, signal sequence, or array of transcription factor binding sites) and a second nucleic acid sequence, wherein the expression control sequence affects transcription and/or translation of the nucleic acid corresponding to the second sequence.
  • a nucleic acid expression control sequence such as a promoter, signal sequence, or array of transcription factor binding sites
  • Recombinant when used with reference to a cell indicates that the cell replicates a heterologous nucleic acid, expresses said nucleic acid or expresses a peptide, heterologous peptide, or protein encoded by a heterologous nucleic acid.
  • Recombinant cells can express genes or gene fragments in either the sense or antisense form that are not found within the native (non-recombinant) form of the cell.
  • Recombinant cells can also express genes that are found in the native form of the cell, but wherein the genes are modified and re-introduced into the cell by artificial means.
  • a “structural gene” is that portion of a gene comprising a DNA segment encoding a protein, polypeptide or a portion thereof, and excluding the 5′ sequence which drives the initiation of transcription.
  • the structural gene may alternatively encode a nontranslatable product.
  • the structural gene may be one which is normally found in the cell or one which is not normally found in the cell or cellular location wherein it is introduced, in which case it is termed a “heterologous gene”.
  • a heterologous gene may be derived in whole or in part from any source known to the art, including a bacterial genome or episome, eukaryotic, nuclear or plasmid DNA, cDNA, viral DNA or chemically synthesized DNA.
  • a structural gene may contain one or more modifications that could effect biological activity or its characteristics, the biological activity or the chemical structure of the expression product, the rate of expression or the manner of expression control. Such modifications include, but are not limited to, mutations, insertions, deletions and substitutions of one or more nucleotides.
  • the structural gene may constitute an uninterrupted coding sequence or it may include one or more introns, bounded by the appropriate splice junctions.
  • the structural gene may be translatable or non-translatable, including in an anti-sense orientation.
  • the structural gene may be a composite of segments derived from a plurality of sources and from a plurality of gene sequences (naturally occurring or synthetic, where synthetic refers to DNA that is chemically synthesized).
  • “Derived from” is used to mean taken, obtained, received, traced, replicated or descended from a source (chemical and/or biological).
  • a derivative may be produced by chemical or biological manipulation (including, but not limited to, substitution, addition, insertion, deletion, extraction, isolation, mutation and replication) of the original source.
  • “Chemically synthesized”, as related to a sequence of DNA, means that portions of the component nucleotides were assembled in vitro.
  • Manual chemical synthesis of DNA may be accomplished using well established procedures (Caruthers, Methodology of DNA and RNA Seauencing, (1983), Weissman (ed.), Praeger Publishers, New York, Chapter 1); automated chemical synthesis can be performed using one of a number of commercially available machines.
  • Optimal alignment of sequences for comparison may be conducted by the local homology algorithm of Smith and Waterman, Adv. Appl. Math. 2:482 (1981), by the homology alignment algorithm of Needleman and Wunsch, J. Mol. Biol. 48:443 (1970), by the search for similarity method of Pearson and Lipman Proc. Natl. Acad. Sci. (U.S.A.) 85: 2444 (1988), by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, Wis.), or by inspection.
  • NCBI Basic Local Alignment Search Tool (Altschul et al., 1990) is available from several sources, including the National Center for Biological Information (NCBI, Bethesda, Md.) and on the Internet, for use in connection with the sequence analysis programs blastp, blastn, blastx, tblastn and tblastx. It can be accessed at htp://www.ncbi.nlm.nih.gov/BLAST/. A description of how to determine sequence identity using this program is available at http://www.ncbi.nlm.nih.gov/BLAST/blast help.html.
  • substantially amino acid identity or “substantial amino acid sequence identity” as applied to amino acid sequences and as used herein denote a characteristic of a polypeptide, wherein the peptide comprises a sequence that has at least 70 percent sequence identity, preferably 80 percent amino acid sequence identity, more preferably 90 percent amino acid sequence identity, and most preferably at least 99 to 100 percent sequence identity as compared to a reference group over region corresponding to the first amino acid following the cytochrome p450 motif GXRXCX(G/A) to the stop codon of the translated peptide.
  • nucleic acid identity or “substantial nucleic acid sequence identity” as applied to nucleic acid sequences and as used herein denote a characteristic of a polynucleotide sequence, wherein the polynucleotide comprises a sequence that has at least 75 percent sequence identity, preferably 81 percent amino acid sequence identity, more preferably at least 91 percent sequence identity, and most preferably at least 99 to 100 percent sequence identity as compared to a reference group over region corresponding to the first nucleic acid following the cytochrome p450 motif GXRXCX(G/A) to the stop codon of the translated peptide.
  • nucleotide sequences are substantially identical if two molecules hybridize to each other under stringent conditions.
  • Stringent conditions are sequence-dependent and will be different in different circumstances.
  • stringent conditions are selected to be about 5° C. to about 20° C., usually about 10° C. to about 15° C., lower than the thermal melting point (Tm) for the specific sequence at a defined ionic strength and pH.
  • Tm is the temperature (under defined ionic strength and pH) at which 50% of the target sequence hybridizes to a matched probe.
  • stringent conditions will be those in which the salt concentration is about 0.02 molar at pH 7 and the temperature is at least about 60° C.
  • stringent conditions will include an initial wash in 6 ⁇ SSC at 42° C. followed by one or more additional washes in 0.2 ⁇ SSC at a temperature of at least about 55° C., typically about 60° C. and often about 65° C.
  • Nucleotide sequences are also substantially identical for purposes of this invention when the polypeptides and/or proteins which they encode are substantially identical.
  • one nucleic acid sequence encodes essentially the same polypeptide as a second nucleic acid sequence
  • the two nucleic acid sequences are substantially identical, even if they would not hybridize under stringent conditions due to degeneracy permitted by the genetic code (see, Darnell et al. (1990) Molecular Cell Biology, Second Edition Scientific American Books W. H. Freeman and Company New York for an explanation of codon degeneracy and the genetic code).
  • Protein purity or homogeneity can be indicated by a number of means well known in the art, such as polyacrylamide gel electrophoresis of a protein sample, followed by visualization upon staining. For certain purposes high resolution may be needed and HPLC or a similar means for purification may be utilized.
  • vector is used in reference to nucleic acid molecules that transfer DNA segment(s) into a cell.
  • a vector may act to replicate DNA and may reproduce independently in a host cell.
  • vector is sometimes used interchangeably with “vector.”
  • expression vector refers to a recombinant DNA molecule containing a desired coding sequence and appropriate nucleic acid sequences necessary for the expression of the operably linked coding sequence in a particular host organism.
  • Nucleic acid sequences necessary for expression in prokaryotes usually include a promoter, an operator (optional), and a ribosome binding site, often along with other sequences.
  • Eucaryotic cells are known to utilize promoters, enhancers, and termination and polyadenylation signals.
  • a nucleic acid may be inserted into plant cells, for example, by any technique such as in vivo inoculation or by any of the known in vitro tissue culture techniques to produce transformed plant cells that can be regenerated into complete plants.
  • the insertion into plant cells may be by in vitro inoculation by pathogenic or non-pathogenic A. tumefaciens. Other such tissue culture techniques may also be employed.
  • Plant tissue includes differentiated and undifferentiated tissues of plants, including, but not limited to, roots, shoots, leaves, pollen, seeds, tumor tissue and various forms of cells in culture, such as single cells, protoplasts, embryos and callus tissue.
  • the plant tissue may be in planta or in organ, tissue or cell culture.
  • Plant cell as used herein includes plant cells in planta and plant cells and protoplasts in culture.
  • cDNA or “complementary DNA” generally refers to a single stranded DNA molecule with a nucleotide sequence that is complementary to an RNA molecule. cDNA is formed by the action of the enzyme reverse transcriptase on an RNA template.
  • RNA was extracted from Nicotiana tissue of converter and non-converter Nicotiana lines. The extracted RNA was then used to create cDNA. Nucleic acid sequences of the present invention were then generated using two strategies.
  • the poly A enriched RNA was extracted from plant tissue and cDNA was made by reverse transcription PCR. The single strand cDNA was then used to create p450 specific PCR populations using degenerate primers plus a oligo d(T) reverse primer.
  • the primer design was based on the highly conserved motifs of p450. Examples of specific degenerate primers are set forth in FIG. 1. Sequence fragments from plasmids containing appropriate size inserts were further analyzed. These size inserts typically ranged from about 300 to about 800 nucleotides depending on which primers were used.
  • a cDNA library was initially constructed.
  • the cDNA in the plasmids was used to create p450 specific PCR populations using degenerate primers plus T7 primer on plasmid as reverse primer.
  • sequence fragments from plasmids containing appropriate size inserts were further analyzed.
  • Nicotiana plant lines known to produce high levels of nornicotine (converter) and plant lines having undetectable levels of nornicotine may be used as starting materials.
  • Leaves can then be removed from plants and treated with ethylene to activate p450 enzymatic activities defined herein.
  • Total RNA is extracted using techniques known in the art.
  • cDNA fragments can then be generated using PCR (RT-PCR) with the oligo d(T) primer as described in FIG. 153.
  • the cDNA library can then be constructed more fully described in examples herein.
  • the conserved region of p450 type enzymes can be used as a template for degenerate primers (FIG. 75). Using degenerate primers, p450 specific bands can be amplified by PCR. Bands indicative for p450 like enzymes can be identified by DNA sequencing. PCR fragments can be characterized using BLAST search, alignment or other tools to identify appropriate candidates.
  • Sequence information from identified fragments can be used to develop PCR primers. These primers in combination of plasmid primers in cDNA library were used to clone full length p450 genes.
  • Large-scale Southern reverse analysis was conducted to examine the differential expression for all fragment clones obtained and in some cases full length clones.
  • these large-scale reverse Southern assays can be conducted using labeled total cDNA's from different tissues as a probe to hybridize with cloned DNA fragments in order to screen all cloned inserts.
  • Nonradioactive and radioactive (p 32 ) Northern blotting assays were also used to characterize clones p450 fragments and full length clones.
  • Peptide specific antibodies were made against several full-length clones by deriving their amino acid sequence and selecting peptide regions that were antigenic and unique relative to other clones. Rabbit antibodies were made to synthetic peptides conjugated to a carrier protein. Western blotting analyses or other immunological methods were performed on plant tissue using these antibodies.
  • Nucleic acid sequences identified as described above can be examined by using virus induced gene silencing technology (VIGS, Baulcombe, Current Opinions in Plant Biology, 1999, 2:109-113).
  • Peptide specific antibodies were made for several full-length clones by deriving their amino acid sequence and selecting peptide regions that were potentially antigenic and were unique relative to other clones. Rabbit antibodies were made to synthetic petides conjugated to a carrier protein. Western blotting analyses were perfomed using these antibodies.
  • RNAi interfering RNA technology
  • RNAi interfering RNA technology
  • the following references which describe this technology are incorporated by reference herein, Smith et al., Nature, 2000, 407:319-320; Fire et al., Nature, 1998, 391:306- 311; Waterhouse et al., PNAS, 1998, 95:13959-13964; Stalberg et al., Plant Molecular Biology, 1993, 23:671- 683; Baulcombe, Current Opinions in Plant Biology, 1999, 2:109-113; and Brigneti et al., EMBO Journal, 1998, 17(22):6739-6746. Plants may be transformed using RNAi techniques, antisense techniques, or a variety of other methods described.
  • Electroporation technology has also been used to transform plants, see WO 87/06614 to Boyce Thompson Institute, U.S. Pat. Nos. 5,472,869 and 5,384,253 both to Dekalb, WO9209696 and WO9321335 both to PGS. All of these transformation patents and publications are incorporated by reference.
  • tissue which is contacted with the foreign genes may vary as well. Such tissue would include but would not be limited to embryogenic tissue, callus tissue type I and II, hypocotyl, meristem, and the like. Almost all plant tissues may be transformed during dedifferentiation using appropriate techniques within the skill of an artisan.
  • Foreign genetic material introduced into a plant may include a selectable marker.
  • the preference for a particular marker is at the discretion of the artisan, but any of the following selectable markers may be used along with any other gene not listed herein which could function as a selectable marker.
  • selectable markers include but are not limited to aminoglycoside phosphotransferase gene of transposon Tn5 (Aph II) which encodes resistance to the antibiotics kanamycin, neomycin and G418, as well as those genes which code for resistance or tolerance to glyphosate; hygromycin; methotrexate; phosphinothricin (bar); imidazolinones, sulfonylureas and triazolopyrimidine herbicides, such as chlorosulfuron; bromoxynil, dalapon and the like.
  • reporter gene In addition to a selectable marker, it may be desirous to use a reporter gene. In some instances a reporter gene may be used without a selectable marker. Reporter genes are genes which are typically not present or expressed in the recipient organism or tissue. The reporter gene typically encodes for a protein which provide for some phenotypic change or enzymatic property. Examples of such genes are provided in K. Weising et al. Ann. Rev. Genetics, 22, 421 (1988), which is incorporated herein by reference. Preferred reporter genes include without limitation glucuronidase (GUS) gene and GFP genes.
  • GUS glucuronidase
  • the expression of the structural gene may be assayed by any means known to the art, and expression may be measured as mRNA transcribed, protein synthesized, or the amount of gene silencing that occurs (see U.S. Pat. No. 5,583,021 which is hereby incorporated by reference). Techniques are known for the in vitro culture of plant tissue, and in a number of cases, for regeneration into whole plants (EP Appln No. 88810309.0). Procedures for transferring the introduced expression complex to commercially useful cultivars are known to those skilled in the art.
  • plant tissues and whole plants can be regenerated therefrom using methods and techniques well-known in the art.
  • the regenerated plants are then reproduced by conventional means and the introduced genes can be transferred to other strains and cultivars by conventional plant breeding techniques.
  • Plants were seeded in pots and grown in a greenhouse for 4 weeks. The 4 week old seedlings were transplanted into individual pots and grown in the greenhouse for 2 months. The plants were watered 2 times a day with water containing 150 ppm NPK fertilizer during growth. The expanded green leaves were detached from plants to do the ethylene treatment described below.
  • Tobacco line 78379 which is a burley tobacco line released by the University of Kentucky was used as a source of plant material.
  • One hundred plants were cultured as standard in the art of growing tobacco and transplanted and tagged with a distinctive number (1-100). Fertilization and field management were conducted as recommended.
  • Nicotiana line 4407 which is a burley line was used as a source of plant material. Uniform and representative plants (100) were selected and tagged. Of the 100 plants 97 were non-converters and three were converters. Plant number 56 had the least amount of conversion (1.2%) and plant number 58 had the highest level of conversion (96%). Self-pollenated seeds and crossed seeds were made with these two plants.
  • Plants from selfed-58 segregated with 3:1 converter to non-converter ratio were identified as homozygous converter and nonconverter plant lines, respectively.
  • the stable conversion of 58-33 was confirmed by analysis of its progenies of next generation.
  • PBLB01 is a burley line developed by ProfiGen, Inc. and was used as a source of plant material. The converter plant was selected from foundation seeds of PBLB01.
  • Green leaves were detached from 2-3 month greenhouse grown plants and sprayed with 0.3% ethylene solution (Prep brand Ethephon (Rhone-Poulenc)). Each sprayed leaf was hung in a curing rack equipped with humidifier and covered with plastic. During the treatment, the sample leaves were periodically sprayed with the ethylene solution. Approximately 24-48 hour post ethylene treatment, leaves were collected for RNA extraction. Another sub-sample was taken for metabolic constituent analysis to determine the concentration of leaf metabolites and more specific constituents of interest such as a variety of alkaloids.
  • alkaloids analysis could be performed as follows. Samples (0.1 g) were shaken at 150 rpm with 0.5 ml 2N NaOH, and a 5 ml extraction solution which contained quinoline as an internal standard and methyl t-butyl ether. Samples were analyzed on a HP 6890 GC equipped with a FID detector. A temperature of 250° C. was used for the detector and injector. An HP column (30 m-0.32 nm-1 m) consisting of fused silica crosslinked with 5% phenol and 95% methyl silicon was used at a temperature gradient of 110-185° C. at 10° C. per minute. The column was operated at 100° C. with a flow rate of 1.7 cm 3 min ⁇ 1 with a split ratio of 40:1 with a 2 l injection volume using helium as the carrier gas.
  • RNA extractions Middle leaves from 2 month old greenhouse grown plants were treated with ethylene as described. The 0 and 24-48 hours samples were used for RNA extraction. In some cases, leaf samples under the senescence process were taken from the plants 10 days post flower-head removal. These samples were also used for extraction. Total RNA was isolated using Rneasy Plant Mini Kit® (Qiagen, Inc., Valencia, Calif.) following manufacturer's protocol.
  • the tissue sample was ground under liquid nitrogen to a fine powder using a DEPC treated mortar and pestle. Approximately 100 milligrams of ground tissue were transferred to a sterile 1.5 ml eppendorf tube. This sample tube was placed in liquid nitrogen until all samples were collected. Then, 450 ⁇ -l of Buffer RLT as provided in the kit (with the addition of Mercaptoethanol) was added to each individual tube. The sample was vortexed vigorously and incubated at 56° C. for 3 minutes. The lysate was then, applied to the QIAshredderTM spin column sitting in a 2-ml collection tube, and centrifuged for 2 minutes at maximum speed. The flow through was collected and 0.5 volume of ethanol was added to the cleared lysate.
  • the sample is mixed well and transferred to an Rneasy® mini spin column sitting in a 2 ml collection tube.
  • the sample was centrifuged for 1 minute at 10,000 rpm.
  • 700 ⁇ l of buffer RW1 was pipetted onto the Rneasy® column and centrifuged for 1 minute at 10,000 rpm.
  • Buffer RPE was pipetted onto the Rneasy® column in a new collection tube and centrifuged for 1 minute at 10,000 rpm.
  • Buffer RPE was again, added to the Rneasy® spin column and centrifuged for 2 minutes at maximum speed to dry the membrane. To eliminate any ethanol carry over, the membrane was placed in a separate collection tube and centrifuged for an additional 1 minute at maximum speed.
  • the Rneasy® column was transferred into a new 1.5 ml collection tube, and 40 ⁇ l of Rnase-free water was pipetted directly onto the Rneasy® membrane. This final elute tube was centrifuged for 1 minute at 10,000 rpm. Quality and quantity of total RNA was analyzed by denatured formaldehyde gel and spectrophotometer.
  • Poly(A)RNA was isolated using OligotexTM poly A+ RNA purification kit (Qiagen Inc.) following manufacture's protocol. About 200 ⁇ g total RNA in 250 ⁇ l maximum volume was used. A volume of 250 ⁇ l of Buffer OBB and 15 ⁇ l of oligotexTM suspension was added to the 250 ⁇ l of total RNA. The contents were mixed thoroughly by pipetting and incubated for 3 minutes at 70° C. on a heating block. The sample was then, placed at room temperature for approximately 20 minutes. The oligotex:mRNA complex was pelleted by centrifugation for 2 minutes at maximum speed. All but 50 ⁇ l of the supernatant was removed from the microcentrifuge tube. The sample was treated further by OBB buffer.
  • the oligotex:mRNA pellet was resuspended in 400 ⁇ l of Buffer OW2 by vortexing. This mix was transferred onto a small spin column placed in a new tube and centrifuged for 1 minute at maximum speed. The spin column was transferred to a new tube and an additional 400 ⁇ l of Buffer OW2 was added to the column. The tube was then centrifuged for 1 minute at maximum speed. The spin column was transferred to a final 1.5 ml microcentrifuge tube. The sample was eluted with 60 ul of hot (70° C.) Buffer OEB. Poly A product was analyzed by denatured formaldehyde gels and spectrophotometric analysis.
  • First strand cDNA was produced using SuperScript reverse transcriptase following manufacturer's protocol (Invitrogen, Carlsbad, Calif.).
  • the poly A+ enriched RNA/oligo dT primer mix consisted of less than 5 ⁇ g of total RNA, 1 ⁇ l of 10 mM DNTP mix, 1 ⁇ l of Oligo d(T) 12-18 (0.5 ⁇ g/ul), and up to 10 ⁇ l of DEPC-treated water. Each sample was incubated at 65° C. for 5 minutes, then placed on ice for at least 1 minute.
  • a reaction mixture was prepared by adding each of the following components in order: 2 ⁇ l 10 ⁇ RT buffer, 4 ⁇ l of 25 mM MgCl2, 2 ⁇ l of 0.1 M DTT, and 1 ⁇ l of RNase OUT Recombinant RNase Inhibitor. An addition of 9 ⁇ l of reaction mixture was pipetted to each RNA/primer mixture and gently mixed. It was incubated at 42° C. for 2 minutes and 1 ⁇ l of Super Script IITM RT was added to each tube. The tube was incubated for 50 minutes at 42° C. The reaction was terminated at 70° C. for 15 minutes and chilled on ice.
  • the sample was collected by centrifugation and 1 ⁇ l of RNase H was added to each tube and incubated for 20 minutes at 37° C.
  • the second PCR was carried out with 200 pmoles of forward primer (degenerate primers as in FIG. 75, SEQ.ID Nos. 149-156) and 100 pmoles reverse primer (mix of 18 nt oligo d(T) followed by 1 random base).
  • Reaction conditions were 94° C. for 2 minutes and then performed 40 cycles of PCR at 94° C. for 1 minute, 45° to 60° C. for 2 minutes, 72° C. for 3 minutes with a 72° C. extension for an extra 10 min.
  • PCR fragments from Example 3 were ligated into a pGEM-T® Easy Vector (Promega, Madison, Wis.) following manufacturer's instructions.
  • the ligated product was transformed into JM109 competent cells and plated on LB media plates for blue/white selection. Colonies were selected and grown in a 96 well plate with 1.2 ml of LB media overnight at 37° C. Frozen stock was generated for all selected colonies.
  • Plasmid DNA from plates were purified using Beckman's Biomeck 2000 miniprep robotics with Wizard SV Miniprep® kit (Promega). Plasmid DNA was eluted with 100 ⁇ l water and stored in a 96 well plate.
  • Plasmids were digested by EcoR1 and were analyzed using 1% agarose gel to confirm the DNA quantity and size of inserts.
  • the plasmids containing a 400-600 bp insert were sequenced using an CEQ 2000 sequencer (Beckman, Fullerton, Calif.). The sequences were aligned with GenBank database by BLAST search. The p450 related fragments were identified and further analyzed. Alternatively, p450 fragments were isolated from substraction libraries. These fragments were also analyzed as described above.
  • a cDNA library was constructed by preparing total RNA from ethylene treated leaves as follows. First, total RNA was extracted from ethylene treated leaves of tobacco line 58-33 using a modified acid phenol and chloroform extraction protocol. Protocol was modified to use one gram of tissue that was ground and subsequently vortexed in 5 ml of extraction buffer (100 mM Tris-HCl, pH 8.5; 200 mM NaCl; 10 mM EDTA; 0.5% SDS) to which 5 ml phenol (pHS.5) and 5 ml chloroform was added. The extracted sample was centrifuged and the supernatant was saved. This extraction step was repeated 2-3 more times until the supernatant appeared clear.
  • extraction buffer 100 mM Tris-HCl, pH 8.5; 200 mM NaCl; 10 mM EDTA; 0.5% SDS
  • poly A+ RNA was used as template to produce a CDNA library employing cDNA synthesis kit, ZAP-cDNA® synthesis kit, and ZAP-cDNA® Gigapack® III gold cloning kit (Stratagene, La Jolla, Calif.). The method involved following the manufacture's protocol as specified. Approximately 8 ⁇ g of poly A+ RNA was used to construct cDNA library. Analysis of the primary library revealed about 2.5 ⁇ 10 6 -1 ⁇ 10 7 pfu. A quality background test of the library was completed by complementation assays using IPTG and X-gal, where recombinant plaques was expressed at more than 100-fold above the background reaction.
  • a more quantitative analysis of the library by random PCR showed that average size of insert cDNA was approximately 1.2 kb.
  • the method used a two-step PCR method as followed.
  • reverse primers were designed based on the preliminary sequence information obtained from p450 fragments.
  • the designed reverse primers and T3 (forward) primers were used amplify corresponding genes from the cDNA library.
  • PCR reactions were subjected to agarose electrophoresis and the corresponding bands of high molecular weight were excised, purified, cloned and sequenced.
  • new primers designed from 5′UTR or the start coding region of p450 as the forward primers together with the reverse primers (designed from 3′UTR of p450) were used in the subsequent PCR to obtain full-length p450 clones.
  • the p450 fragments were generated by PCR amplification from the constructed cDNA library as described in Example 3 with the exception of the reverse primer.
  • the T7 primer located on the plasmid downstream of cDNA inserts was used as a reverse primer. PCR fragments were isolated, cloned and sequenced as described in Example 4.
  • Full-length p450 genes were isolated by PCR method from constructed cDNA library. Gene specific reverse primers (designed from the downstream sequence of p450 fragments) and a forward primer (T3 on library plasmid) were used to clone the full length genes. PCR fragments were isolated, cloned and sequenced. If necessary, second step PCR was applied. In the second step, new forward primers designed from 5′UTR of cloned p450s together with the reverse primers designed from 3′UTR of p450 clones were used in the subsequent PCR reactions to obtain full-length p450 clones. The clones were subsequently sequenced.
  • Nonradioactive large scale reverse southern blotting assays were performed on all p450 clones identified in above examples to detect the differential expression. It was observed that the level of expression among different p450 clusters was very different. Further real time detection was conducted on those with high expression.
  • Probe was produced by biotin-tail labeling a single strand cDNA derived from poly A+ enriched RNA generated in above step. This labeled single strand cDNA was generated by RT-PCR of the converter and nonconverter total RNA (Invitrogen) as described in Example 3 with the exception of using biotinalyted oligo dT as a primer (Promega). These were used as a probe to hybridize with cloned DNA.
  • the inserts were PCR amplified from each plasmid using the sequences located on both arms of p-GEM plasmid, T3 and SP6, as primers.
  • the PCR products were analyzed by running on a 96 well Ready-to-run agarose gels. The confirmed inserts were dotted on two nylon membranes. One membrane was hybridized with converter probe and the other with nonconverter probe.
  • the membranes were hybridized and washed following manufacture's instruction with the modification of washing stringency (Enzo MaxSenceTM kit, Enzo Diagnostics, Inc, Farmingdale, N.Y.).
  • the membranes were prehybridized with hybridization buffer (2 ⁇ SSC buffered formamide, containing detergent and hybridization enhancers) at 42° C. for 30 min and hybridized with 10 ⁇ l denatured probe overnight at 42° C.
  • the membranes then were washed in 1 ⁇ hybridization wash buffer 1 time at room temperature for 10 min and 4 times at 68° C. for 15 min. The membranes were ready for the detection.
  • a random priming method was used to prepare probes from cloned p450 (MegaprimeTM DNA Labelling Systems, Amersham Biosciences).
  • RNA samples were prepared from ethylene treated and non-treated fresh leaves of several pairs of tobacco lines. In some cases poly A+ enriched RNA was used. Approximately 15 ⁇ g total RNA or 1.8 ⁇ g mRNA (methods of RNA and mRNA extraction as described in Example 5) were brought to equal volume with DEPC H 2 O (5-10 ⁇ l). The same volume of loading buffer (1 ⁇ MOPS; 18.5% Formaldehyde; 50% Formamide; 4% Ficoll400; Bromophenolblue) and 0.5 ⁇ l EtBr (0.5 ⁇ g/ ⁇ l) were added. The samples were subsequently denatured in preparation for separation of the RNA by electrophoresis.
  • RNA samples were subjected to electrophoresis on a formaldehyde gel (1% Agarose, 1 ⁇ MOPS, 0.6 M Formaldehyde) with 1 ⁇ MOP buffer (0.4 M Morpholinopropanesulfonic acid; 0.1 M Na-acetate-3 ⁇ H2O; 10 mM EDTA; adjust to pH 7.2 with NaOH).
  • RNA was transferred to a Hybond-N+ membrane (Nylon, Amersham Pharmacia Biotech) by capillary method in 10 ⁇ SSC buffer (1.5 M NaCl; 0.15 M Na-citrate) for 24 hours.
  • Membranes with RNA samples were UV-crosslinked (auto crosslink setting, 254 nm, Stratagene, Stratalinker) before hybridization.
  • the membrane was prehybridized for 1-4 hours at 42° C. with 5-10 ml prehybridization buffer (5 ⁇ SSC; 50% Formamide; 5 ⁇ Denhardt's-solution; 1% SDS; 100 ⁇ g/ml heat-denatured sheared non- homologous DNA). Old prehybridization buffer was discarded, and new prehybridization buffer and probe were added. The hybridization was carried out over night at 42° C. The membrane was washed for 15 minutes with 2 ⁇ SSC at room temperature, followed by a wash with 2 ⁇ SSC.
  • 5 ⁇ SSC 50% Formamide
  • 5 ⁇ Denhardt's-solution 1% SDS
  • SDS 100 ⁇ g/ml heat-denatured sheared non- homologous DNA
  • a major focus of the invention was the discovery of novel genes that may be induced as a result of ethylene treatment or play a key role in tobacco leaf quality and constituents. As illustrated in the table below, Northern blots and reverse Southern Blot were useful in determining which genes were induced by ethylene treatment relative to non-induced plants. Interestingly, not all fragments were affected similarly in the converter and nonconverter. The cytochrome p450 fragments of interest were partially sequenced to determine their structural relatedness. This information was used to subsequently isolate and characterize full length gene clones of interest.
  • Peptide regions corresponding to 20-22 amino acids in length from three p450 clones were selected for 1) having lower or no homology to other clones and 2) having good hydrophilicity and antigenicity.
  • the amino acid sequences of the peptide regions selected from the respective p450 clones are listed below.
  • the synthesized peptides were conjugated with KHL and then injected into rabbits. Antisera were collected 2 and 4 weeks after the 4 th injection (Alpha Diagnostic Intl. Inc. San Antonio, Tex.).
  • D234-AD1 DIDGSKSKLVKAHRKIDEILG D90a-BB3 RDAFREKETFDENDVEELNY
  • Antisera were examined for crossreactivity to target proteins from tobacco plant tissue by Western Blot analysis. Crude protein extracts were obtained from ethylene treated (0 to 40 hours) middle leaves of converter and nonconverter lines. Protein concentrations of the extracts were determined using RC DC Protein Assay Kit (BIO-RAD) following the manufacturer's protocol.
  • Spectrophotometry was used to estimate the concentration of starting double stranded DNA following the manufacturer's protocol (Beckman Coulter).
  • the template was diluted with water to the appropriate concentration, denatured by heating at 95° C. for 2 minutes, and subsequently placed on ice.
  • the sequencing reaction was prepared on ice using 0.5 to 10 ⁇ l of denatured DNA template, 2 ⁇ l of 1.6 pmole of the forward primer, 8 ⁇ l of DTCS Quick Start Master Mix and the total volume brought to 20 ⁇ l with water.
  • the thermocycling program consisted of 30 cycles of the follow cycle: 96° C. for 20 seconds, 50° C. for 20 seconds, and 60° C. for 4 minutes followed by holding at 4° C.
  • the sequence was stopped by adding 5 ⁇ l of stop buffer (equal volume of 3M NaOAc and 100 mM EDTA and 1 ⁇ l of 20 mg/ml glycogen).
  • the sample was precipitated with 60 ⁇ l of cold 95% ethanol and centrifuged at 6000 g for 6 minutes. Ethanol was discarded. The pellet was 2 washes with 200 ⁇ l of cold 70% ethanol. After the pellet was dry, 40 ⁇ l of SLS solution was added and the pellet was resuspended. A layer of mineral oil was over laid. The sample was then, placed on the CEQ 8000 Automated Sequencer for further analysis.
  • nucleic acid sequence was re-sequenced in both directions using forward primers to the FXPERF or GRRXCP(A/G) region of the p450 gene or reverse primers to either the plasmid or poly A tail. All sequencing was performed at least twice in both directions.
  • nucleic acid sequences of cytochrome p450 fragments were compared to each other from the coding region corresponding to the first nucleic acid after the region encoding the GRRXCP(A/G) motif through to the stop codon.
  • This region was selected as an indicator of genetic diversity among p450 proteins.
  • the genes could be placed into distinct sequences groups based on their sequence identity. It was found that the best unique grouping of p450 members was determined to be those sequences with 75% nucleic acid identity or greater (shown in Table I). Reducing the percentage identity resulted in significantly larger groups.
  • a preferred grouping was observed for those sequences with 81% nucleic acid identity or greater, a more preferred grouping 91% nucleic acid identity or greater, and a most preferred grouping for those sequences 99% nucleic acid identity of greater.
  • Most of the groups contained at least two members and frequently three or more members. Others were not repeatedly discovered suggesting that approach taken was able to isolated both low and high expressing mRNA in the tissue used.
  • cytochrome p450 groups were found to contain nucleic acid sequence identity to previously tobacco cytochrome genes that genetically distinct from that within the group.
  • Group 23 showed nucleic acid identity, within the parameters used for Table I, to prior GenBank sequences of GI:1171579 (CAA64635) and GI:14423327 (or AAK62346) by Czernic et al and Ralston et al, respectively.
  • GI:1171579 had nucleic acid identity to Group 23 members ranging 96.9% to 99.5% identity to members of Group 23 while GI:14423327 ranged 95.4% to 96.9% identity to this group.
  • the members of Group 31 had nucleic acid identity ranging from 76.7% to 97.8% identity to the GenBank reported sequence of GI:14423319 (AAK62342) by Ralston et al. None of the other p450 identity groups of Table 1 contained parameter identity, as used in Table 1, to Nicotiana p450s genes reported by Ralston et al, Czernic et al., Wang et al or LaRosa and Smigocki.
  • the amino acid sequences of nucleic acid sequences obtained for cytochrome p450 fragments from Example 8 were deduced.
  • the deduced region corresponded to the amino acid immediately after the GXRXCP(A/G) sequence motif to the end of the carboxyl-terminus, or stop codon.
  • a unique grouping was observed for those sequences with 70% amino acid identity or greater.
  • a preferred grouping was observed for those sequences is with 80% amino acid identity or greater, more preferred with 90% amino acid identity or greater, and a most preferred grouping for those sequences 99% amino acid identity of greater.
  • the groups and corresponding amino acid sequences of group members are shown in FIG. 2. Several of the unique nucleic acid sequences were found to have complete amino acid identity to other fragments and therefore only one member with the identical amino acid was reported.
  • At least one member of each amino acid identity group was selected for gene cloning and functional studies using plants.
  • group members that are differentially affected by ethylene treatment or other biological differences as assessed by Northern and Southern analysis were selected for gene cloning and functional studies.
  • peptide specific antibodies will be prepared on sequence identity and differential sequence.
  • All p450 genes were characterized for amino acid identity using a BLAST program comparing their full length sequences to each other and to known tobacco genes.
  • the program used the NCBI special BLAST tool (Align two sequences (b12seq), http://www.ncbi.nlm.nih.gov/blast/b12seq/b12.html). Two sequences were aligned under BLASTN without filter for nucleic acid sequences and BLASTP for amino acid sequences. Based on their percentage amino acid identity, each sequence was grouped into identity groups where the grouping contained members that shared at least 85% identity with another member.
  • D120-AH4 (SEQ. ID. No. 180); D121-AA8 (SEQ. ID. No. 182), D122-AF10 (SEQ. ID. No. 184); D103-AH3 (SEQ. ID. No. 222); D208-AC8 (SEQ. ID. No. 218); D-235-ABI (SEQ. ID. No. 246) 2 D244-AD4 (SEQ. ID. No. 250); D244-AB6 (SEQ. ID. No. 274) ; D285-AA8; D285- AB9; D268-AE2 (SEQ. ID. No. 270) 3 D100A-AC3 (SEQ. ID. No.
  • D100A-BE2 4 D205-BE9 (SEQ. ID. No. 276); D205-BG9 (SEQ. ID. No. 202); D205-AH4 (SEQ. ID. No. 294) 5 D259-AB9 (SEQ. ID. No. 260) ; D257-AE4 (SEQ. ID. No. 268) ; D147-AD3 (SEQ. ID. No. 194) 6 D249-AE8 (SEQ. ID. No. 256); D-248-AA6 (SEQ. ID. No. 254) 7 D233-AG7 (SEQ. ID. No. 266; D224-BD11 (SEQ. ID. No. 240); DAF10 8 D105-AD6 (SEQ. ID.
  • D215-AB5 SEQ. ID. No. 220
  • D135-AE1 SEQ. ID. No. 190
  • 9 D87A-AF3 SEQ. ID. No. 216
  • D210-BD4 SEQ. ID. No. 262
  • 10 D89-AB1 SEQ. ID. No. 150
  • D89-AD2 SEQ. ID. No. 152
  • 163-AG11 SEQ. ID. No. 198
  • 163-AF12 SEQ. ID. No. 196
  • D267-AF10 SEQ. ID. No. 296
  • D96-AC2 SEQ. ID. No. 160
  • D96-AB6 SEQ. ID. No.
  • D207-AA5 SEQ. ID. No. 204
  • D207-AB4 SEQ. ID. No. 206
  • D207-AC4 SEQ. ID. No. 208
  • 12 D98-AG1 SEQ. ID. No. 164
  • D98-AA1 SEQ. ID. No. 162
  • D209-AA11 D209-AH10
  • D209-AH12 SEQ. ID. No. 232
  • D90a-BB3 SEQ. ID. No. 154) 14 D129-AD10 (SEQ. ID. No. 188); D104A-AE8 (SEQ. ID. No.
  • D228-AH8 SEQ. ID. No. 244
  • D228-AD7 SEQ. ID. No. 241
  • D250- AC11 SEQ. ID. No. 258
  • D247-AH1 SEQ. ID. No. 252
  • 16 D128-AB7 SEQ. ID. No. 186
  • D243-AA2 SEQ. ID. No. 248
  • D110-AF12 SEQ. ID. No. 176)
  • 18 D221-BB8 SEQ. ID. No. 234) 19 D222-BH4 (SEQ. ID. No.
  • the full length genes were further grouped based on the highly conversed amino acid homology between UXXRXXZ p450 domain and GXRXC p450 domain near the end the carboxyl-terminus. As shown in FIG. 3, individual clones were aligned for their sequence homology between the conserved domains relative to each other and placed in distinct identity groups. In several cases, although the nucleic acid sequence of the clone was unique, the amino acid sequence for the region was identical. The preferred grouping was observed for those sequences with 90% amino acid identity or greater, a more preferred group had 95% amino acid identity or greater, and a most preferred grouping had those sequences 99% amino acid identity of greater. The final grouping was similar to that based on the percent identity for the entire amino acid sequence of the clones except for Group 17 (of Table III) which was divided into two distinct groups.
  • D120-AH4 (SEQ. ID. No. 180); D121-AA8 (SEQ. ID. No. 182), D122-AF10 (SEQ. ID. No. 184); D103-AH3 (SEQ. ID. No. 222); D208-AC8 (SEQ. ID. No. 218); D-235-ABI (SEQ. ID. No. 246) 2 D244-AD4 (SEQ. ID. No. 250); D244-AB6 (SEQ. ID. No. 274); D285-AA8; D285-AB9; D268-AE2 (SEQ. ID. No. 270) 3 D100A-AC3 (SEQ. ID. No.
  • D100A-BE2 4 D205-BE9 (SEQ. ID. No. 276); D205-BG9 (SEQ. ID. No. 202); D205-AH4 (SEQ. ID. No. 294) 5 D259-AB9 (SEQ. ID. No. 260) ; D257-AE4 (SEQ. ID. No. 268); D147-AD3 (SEQ. ID. No. 194) 6 D249-AE8 (SEQ. ID. No. 256); D-248-AA6 (SEQ. ID. No. 254) 7 D233-AG7 (SEQ. ID. No. 266; D224-BD11 (SEQ. ID. No. 240) ; DAF10 8 D105-AD6 (SEQ.
  • D215-AB5 SEQ. ID. No. 220
  • D135-AE1 SEQ. ID. No. 190
  • 9 D87A-AF3 SEQ. ID. No. 216
  • D210-BD4 SEQ. ID. No. 262
  • 10 D89-AB1 SEQ. ID. No. 150
  • D89-AD2 SEQ. ID. No. 152
  • 163-AG11 SEQ. ID. No. 198
  • 163-AF12 SEQ. ID. No. 196
  • 11 D267-AF10 SEQ. ID. No. 296
  • D96-AC2 SEQ. ID. No. 160
  • D96-AB6 SEQ. ID. No.
  • D207-AA5 SEQ. ID. No. 204
  • D207-AB4 SEQ. ID. No. 206
  • D207-AC4 SEQ. ID. No. 208
  • 12 D98-AG1 SEQ. ID. No. 164
  • D98-AA1 SEQ. ID. No. 162
  • D209-AA11 D209-AH10
  • D209-AH12 SEQ. ID. No. 232
  • D90a-BB3 SEQ. ID. No. 154) 14 D129-AD10 (SEQ. ID. No. 188); D104A-AE8 (SEQ. ID. No.
  • D228-AH8 SEQ. ID. No. 244
  • D228-AD7 SEQ. ID. No. 241
  • D250-AC11 SEQ. ID. No. 258
  • D247-AH1 SEQ. ID. No. 252
  • 16 D128-AB7 SEQ. ID. No. 186
  • D243-AA2 SEQ. ID. No. 248
  • D110-AF12 SEQ. ID. No. 176)
  • 18 D221-BB8 SEQ. ID. No. 234) 19 D222-BH4 (SEQ. ID. No.
  • Nicotiana Cytochrome P450 Fragments and Clones in Altered Regulartion of Tobacco Properties
  • Transgenic tobacco plants are generated by a variety of transformation systems that incorporate nucleic acid fragments or full length genes, selected from those reported herein, in orientations for either down-regulation, for example anti-sense orientation, or over-expression for example, sense orienation.
  • nucleic acid sequence that encodes the entire or a functional part or amino acide sequence of the full-length genes described in this invention are desired that are effective for increasing the expression of a certain enzyme and thus resulting in phenotypic effect within Nicotiana.
  • Nicotiana lines that are homozygous lines are obtained through a series of backcrossing and assessed for phenotypic changes including, but not limited to, analysis of endogenous p450 RNA, transcripts, p450 expressed peptides and concentrations of plant metabolites using techniques commonly avaiable to one having ordinary skill in the art.

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Abstract

The present invention relates to p450 enzymes and nucleic acid sequences encoding p450 enzymes in Nicotiana, and methods of using those enzymes and nucleic acid sequences to alter plant phenotypes.

Description

  • The present invention relates to nucleic acid sequences encoding cytochrome p450 enzymes (hereinafter referred to as p450 and p450 enzymes) in Nicotiana plants and methods for using those nucleic acid sequences to alter plant phenotypes. [0001]
    • BACKGROUND
  • Cytochrome p450s catalyze enzymatic reactions for a diverse range of chemically dissimilar substrates that include the oxidative, peroxidative and reductive metabolism of endogenous and xenobiotic substrates. In plants, p450s participate in biochemical pathways that include the synthesis of plant products such as phenylpropanoids, alkaloids, terpenoids, lipids, cyanogenic glycosides, and glucosinolates (Chappel, Annu. Rev. Plant Physiol. Plant Mol. Biol. 198, 49:311-343). Cytochrome p450s, also known as p450 heme-thiolate proteins, usually act as terminal oxidases in multi-component electron transfer chains, called p450-containing monooxygenase systems. Specific reactions catalyzed include demethylation, hydroxylation, epoxidation, N-oxidation, sulfooxidation, N-, S-, and O-dealkylations, desulfation, deamination, and reduction of azo, nitro, and N-oxide groups. [0002]
  • The diverse role of Nicotiana plant p450 enzymes has been implicated in effecting a variety of plant metabolites such as phenylpropanoids, alkaloids, terpenoids, lipids, cyanogenic glycosides, glucosinolates and a host of other chemical entities. During recent years, it is becoming apparent that some p450 enzymes can impact the composition of plant metabolites in plants. For example, it has been long desired to improve the flavor and aroma of certain plants by altering its profile of selected fatty acids through breeding; however very little is known about mechanisms involved in controlling the levels of these leaf constituents. The down regulation of p450 enzymes associated with the modification of fatty acids may facilitate accumulation of desired fatty acids that provide more preferred leaf phenotypic qualities. The function of p450 enzymes and their broadening roles in plant constituents is still being discovered. For instance, a special class of p450 enzymes was found to catalyze the breakdown of fatty acid into volatile C6- and C9-aldehydes and -alcohols that are major contributors of “fresh green” odor of fruits and vegetables. The level of other novel targeted p450s may be altered to enhance the qualities of leaf constituents by modifying lipid composition and related break down metabolites in Nicotiana leaf. Several of these constituents in leaf are affected by senescence that stimulates the maturation of leaf quality properties. Still other reports have shown that p450s enzymes are play a functional role in altering fatty acids that are involved in plant-pathogen interactions and disease resistance. [0003]
  • In other instances, p450 enzymes have been suggested to be involved in alkaloid biosynthesis. Nornicotine is a minor alkaloid found in [0004] Nicotiana tabaceum. It has been postulated that it is produced by the p450 mediated demethylation of nicotine followed by acylation and nitrosation at the N position thereby producing a series of N-acylnonicotines and N-nitrosonornicotines. N-demethylation, catalyzed by a putative p450 demethylase, is thought to be a primary source of nornicotine biosyntheses in Nicotiana. While the enzyme is believed to be microsomal, thus far a nicotine demethylase enzyme has not been successfully purified, nor have the genes involved been isolated.
  • Furthermore, it is hypothesized but not proven that the activity of p450 enzymes is genetically controlled and also strongly influenced by environment factors. For example, the demethylation of nicotine in Nicotiana is thought to increase substantially when the plants reach a mature stage. Furthermore, it is hypothesized yet not proven that the demethylase gene contains a transposable element that can inhibit translation of RNA when present. [0005]
  • The large multiplicity of p450 enzyme forms, their differing structure and function have made their research on Nicotiana p450 enzymes very difficult before the enclosed invention. In addition, cloning of p450 enzymes has been hampered at least in part because these membrane-localized proteins are typically present in low abundance and often unstable to purification. Hence, a need exists for the identification of p450 enzymes in plants and the nucleic acid sequences associated with those p450 enzymes. In particular, only a few cytochrome p450 proteins have been reported in Nicotiana. The inventions described herein entail the discovery of a substantial number of cytochrome p450 fragments that correspond to several groups of p450 species based on their sequence identity. [0006]
    • SUMMARY
  • The present invention is directed to plant p450 enzymes. The present invention is further directed to plant p450 enzymes from Nicotiana. The present invention is also directed to p450 enzymes in plants whose expression is induced by ethylene and/or plant senescence. The present invention is yet further directed to nucleic acid sequences in plants having enzymatic activities, for example, being categorized as oxygenase, demethylase and the like, or other and the use of those sequences to reduce or silence the expression or over-expression of these enzymes. The invention also relates to p450 enzymes found in plants containing higher nornicotine levels than plants exhibiting lower nornicotine levels. [0007]
  • In one aspect, the invention is directed to nucleic acid sequences as set forth in SEQ. ID. Nos. 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239, 241, 243, 245, 247, 249, 251, 253, 255, 257, 259, 261, 263, 265, 267, 269, 271, 273, 275, 277, 279, 281, 283, 285, 287, 289, 291, 293, 295 and 297. [0008]
  • In a second related aspect, those fragments containing greater than 75% identity in nucleic acid sequence were placed into groups dependent upon their identity in a region corresponding to the first nucleic acid following the cytochrome p450 motif GXRXCX(G/A) to the stop codon. The representative nucleic acid groups and respective species are shown in Table I. [0009]
  • In a third aspect, the invention is directed to amino acid sequences as set forth in SEQ. ID. Nos. 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292, 294, 296 and 298. [0010]
  • In a fourth related aspect, those fragments containing greater than 71% identity in amino acid sequence were placed into groups dependent upon their identity to each other in a region corresponding to the first amino acid following the cytochrome p450 motif GXRXCX(G/A) to the stop codon. The representative amino acid groups and respective species are shown in Table II. [0011]
  • In a fifth aspect, the invention is directed to amino acid sequences of full length genes as set forth in SEQ. ID. Nos. 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292, 294, 296 and 298. [0012]
  • In a sixth related aspect, those full length genes containing 85% or greater identity in amino acid sequence were placed into groups dependent upon the identity to each other. The representative amino acid groups and respective species are shown in Table III. [0013]
  • In a seventh aspect, the invention is directed to amino acid sequences of the fragments set forth in SEQ. ID. Nos. 299-357. [0014]
  • In the eighth related aspect, those fragments containing 90% or greater identity in amino acid sequence were placed into groups dependent upon their identity to each other in a region corresponding to the first cytochrome p450 domain, UXXRXXZ, to the third cytochrome domain, GXRXO, where U is E or K, X is any amino acid and Z is R, T, S or M. The representative amino acid groups respective species shown in Table IV. [0015]
  • In a ninth related aspect, the reduction or elimination or over-expression of p450 enzymes in Nicotiana plants may be accomplished transiently using RNA viral systems. [0016]
  • Resulting transformed or infected plants are assessed for phenotypic changes including, but not limited to, analysis of endogenous p450 RNA transcripts, p450 expressed peptides, and concentrations of plant metabolites using techniques commonly available to one having ordinary skill in the art. [0017]
  • In a tenth important aspect, the present invention is also directed to generation of trangenic Nicotiana lines that have altered p450 enzyme activity levels. In accordance with the invention, these transgenic lines include nucleic acid sequences that are effective for reducing or silencing or increasing the expression of certain enzyme thus resulting in phenotypic effects within Nicotiana. Such nucleic acid sequences include SEQ. ID. Nos. 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239, 241, 243, 245, 247, 249, 251, 253, 255, 257, 259, 261, 263, 265, 267, 269, 271, 273, 275, 277, 279, 281, 283, 285, 287, 289, 291, 293, 295 and 297. [0018]
  • In a very important eleventh aspect of the invention, plant cultivars including nucleic acids of the present invention in a down regulation capacity using either full length genes or fragments thereof or in an over-expression capacity using full length genes will have altered metabolite profiles relative to control plants. [0019]
  • In a twelfth aspect of the invention, plant cultivars including nucleic acid of the present invention using either full length genes or fragments thereof in modifying the biosynthesis or breakdown of metabolites derived from the plant or external to the plants, will have use in tolerating certain exogenous chemicals or plant pests. Such nucleic acid sequences include SEQ ID. Nos. 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239, 241, 243, 245, 247, 249, 251, 253, 255, 257, 259, 261, 263, 265, 267, 269, 271, 273, 275, 277, 279, 281, 283, 285, 287, 289, 291, 293, 295 and 297. [0020]
  • In a thirteenth aspect, the present invention is directed to the screening of plants, more preferably Nicotiana, that contain genes that have substantial nucleic acid identity to the taught nucleic acid sequence. The use of the invention would be advantageous to identify and select plants that contain a nucleic acid sequence with exact or substantial identity where such plants are part of a breeding program for traditional or transgenic varieties, a mutagenesis program, or naturally occurring diverse plant populations. The screening of plants for substantial nucleic acid identity may be accomplished by evaluating plant nucleic acid materials using a nucleic acid probe in conjunction with nucleic acid detection protocols including, but not limited to, nucleic acid hybridization and PCR analysis. The nucleic acid probe may consist of the taught nucleic acid sequence or fragment thereof corresponding to SEQ ID 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239, 241, 243, 245, 247, 249, 251, 253, 255, 257, 259, 261, 263, 265, 267, 269, 271, 273, 275, 277, 279, 281, 283, 285, 287, 289, 291, 293, 295 and 297. [0021]
  • In a fourteenth aspect, the present invention is directed to the identification of plant genes, more preferably Nicotiana, that share substantial amino acid identity corresponding to the taught nucleic acid sequence. The identification of plant genes including both cDNA and genomic clones, those cDNAs and genomic clones, more preferably from Nicotiana may be accomplished by screening plant cDNA libraries using a nucleic acid probe in conjunction with nucleic acid detection protocols including, but not limited to, nucleic acid hybridization and PCR analysis. The nucleic acid probe may be comprised of nucleic acid sequence or fragment thereof corresponding to [0022] SEQ ID 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 143, 145 and 147.
  • In an alterative fifteenth aspect, cDNA expression libraries that express peptides may be screened using antibodies directed to part or all of the taught amino acid sequence. Such amino acid sequences include [0023] SEQ ID 2, 4, 8, 9, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 144, 146, 148.
  • In a sixteenth important aspect, the present invention is also directed to generation of transgenic Nicotiana lines that have over-expression of p450 enzyme activity levels. In accordance with the invention, these transgenic lines include all nucleic acid sequences encoding the amino acid sequences of full length genes that are effective for increasing the expression of certain enzyme thus resulting in phenotypic effects within Nicotiana. Such amino acid sequences include SEQ. ID. 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292, 294, 296 and 298.[0024]
    • BRIEF DESCRIPTION OF DRAWINGS
  • FIG. 1 shows nucleic acid SEQ. ID. No.:1 and amino acid SEQ. ID. No.:2. [0025]
  • FIG. 2 shows nucleic acid SEQ. ID. No.:3 and amino acid SEQ. ID. No.:4. [0026]
  • FIG. 3 shows nucleic acid SEQ. ID. No.:5 and amino acid SEQ. ID. No.:6. [0027]
  • FIG. 4 shows nucleic acid SEQ. ID. No.:7 and amino acid SEQ. ID. No.:8. [0028]
  • FIG. 5 shows nucleic acid SEQ. ID. No.:9 and amino acid SEQ. ID. No.:10. [0029]
  • FIG. 6 shows nucleic acid SEQ. ID. No.:11 and amino acid SEQ. ID. No.:12. [0030]
  • FIG. 7 shows nucleic acid SEQ. ID. No.:13 and amino acid SEQ. ID. No.:14. [0031]
  • FIG. 8 shows nucleic acid SEQ. ID. No.:15 and amino acid SEQ. ID. No.:16. [0032]
  • FIG. 9 shows nucleic acid SEQ. ID. No.:17 and amino acid SEQ. ID. No.:18. [0033]
  • FIG. 10 shows nucleic acid SEQ. ID. No.:19 and amino acid SEQ. ID. No.:20. [0034]
  • FIG. 11 shows nucleic acid SEQ. ID. No.:21 and amino acid SEQ. ID. No.:22. [0035]
  • FIG. 12 shows nucleic acid SEQ. ID. No.:23 and amino acid SEQ. ID. No.:24. [0036]
  • FIG. 13 shows nucleic acid SEQ. ID. No.:25 and amino acid SEQ. ID. No.:26. [0037]
  • FIG. 14 shows nucleic acid SEQ. ID. No.:27 and amino acid SEQ. ID. No.:28. [0038]
  • FIG. 15 shows nucleic acid SEQ. ID. No.:29 and amino acid SEQ. ID. No.:30. [0039]
  • FIG. 16 shows nucleic acid SEQ. ID. No.:31 and amino acid SEQ. ID. No.:32. [0040]
  • FIG. 17 shows nucleic acid SEQ. ID. No.:33 and amino acid SEQ. ID. No.:34. [0041]
  • FIG. 18 shows nucleic acid SEQ. ID. No.:35 and amino acid SEQ. ID. No.:36. [0042]
  • FIG. 19 shows nucleic acid SEQ. ID. No.:37 and amino acid SEQ. ID. No.:38. [0043]
  • FIG. 20 shows nucleic acid SEQ. ID. No.:39 and amino acid SEQ. ID. No.:40. [0044]
  • FIG. 21 shows nucleic acid SEQ. ID. No.:41 and amino acid SEQ. ID. No.:42. [0045]
  • FIG. 22 shows nucleic acid SEQ. ID. No.:43 and amino acid SEQ. ID. No.:44. [0046]
  • FIG. 23 shows nucleic acid SEQ. ID. No.:45 and amino acid SEQ. ID. No.:46. [0047]
  • FIG. 24 shows nucleic acid SEQ. ID. No.:47 and amino acid SEQ. ID. No.:48. [0048]
  • FIG. 25 shows nucleic acid SEQ. ID. No.:49 and amino acid SEQ. ID. No.:50. [0049]
  • FIG. 26 shows nucleic acid SEQ. ID. No.:51 and amino acid SEQ. ID. No.:52. [0050]
  • FIG. 27 shows nucleic acid SEQ. ID. No.:53 and amino acid SEQ. ID. No.:54. [0051]
  • FIG. 28 shows nucleic acid SEQ. ID. No.:55 and amino acid SEQ. ID. No.:56. [0052]
  • FIG. 29 shows nucleic acid SEQ. ID. No.:57 and amino acid SEQ. ID. No.:58. [0053]
  • FIG. 30 shows nucleic acid SEQ. ID. No.:59 and amino acid SEQ. ID. No.:60. [0054]
  • FIG. 31 shows nucleic acid SEQ. ID. No.:61 and amino acid SEQ. ID. No.:62. [0055]
  • FIG. 32 shows nucleic acid SEQ. ID. No.:63 and amino acid SEQ. ID. No.:64. [0056]
  • FIG. 33 shows nucleic acid SEQ. ID. No.:65 and amino acid SEQ. ID. No.:66. [0057]
  • FIG. 34 shows nucleic acid SEQ. ID. No.:67 and amino acid SEQ. ID. No.:68. [0058]
  • FIG. 35 shows nucleic acid SEQ. ID. No.:69 and amino acid SEQ. ID. No.:70. [0059]
  • FIG. 36 shows nucleic acid SEQ. ID. No.:71 and amino acid SEQ. ID. No.:72. [0060]
  • FIG. 37 shows nucleic acid SEQ. ID. No.:73 and amino acid SEQ. ID. No.:74. [0061]
  • FIG. 38 shows nucleic acid SEQ. ID. No.:75 and amino acid SEQ. ID. No.:76. [0062]
  • FIG. 39 shows nucleic acid SEQ. ID. No.:77 and amino acid SEQ. ID. No.:78. [0063]
  • FIG. 40 shows nucleic acid SEQ. ID. No.:79 and amino acid SEQ. ID. No.:80. [0064]
  • FIG. 41 shows nucleic acid SEQ. ID. No.:81 and amino acid SEQ. ID. No.:82. [0065]
  • FIG. 42 shows nucleic acid SEQ. ID. No.:83 and amino acid SEQ. ID. No.:84. [0066]
  • FIG. 43 shows nucleic acid SEQ. ID. No.:85 and amino acid SEQ. ID. No.:86. [0067]
  • FIG. 44 shows nucleic acid SEQ. ID. No.:87 and amino acid SEQ. ID. No.:88. [0068]
  • FIG. 45 shows nucleic acid SEQ. ID. No.:89 and amino acid SEQ. ID. No.:90. [0069]
  • FIG. 46 shows nucleic acid SEQ. ID. No.:91 and amino acid SEQ. ID. No.:92. [0070]
  • FIG. 48 shows nucleic acid SEQ. ID. No.:95 and amino acid SEQ. ID. No.:96. [0071]
  • FIG. 49 shows nucleic acid SEQ. ID. No.:97 and amino acid SEQ. ID. No.:98. [0072]
  • FIG. 50 shows nucleic acid SEQ. ID. No.:99 and amino acid SEQ. ID. No.:100. [0073]
  • FIG. 51 shows nucleic acid SEQ. ID. No.:101 and amino acid SEQ. ID. No.:102. [0074]
  • FIG. 52 shows nucleic acid SEQ. ID. No.:103 and amino acid SEQ. ID. No.:104. [0075]
  • FIG. 53 shows nucleic acid SEQ. ID. No.:105 and amino acid SEQ. ID. No.:106. [0076]
  • FIG. 54 shows nucleic acid SEQ. ID. No.:107 and amino acid SEQ. ID. No.:108. [0077]
  • FIG. 55 shows nucleic acid SEQ. ID. No.:109 and amino acid SEQ. ID. No.:110. [0078]
  • FIG. 56 shows nucleic acid SEQ. ID. No.:111 and amino acid SEQ. ID. No.:112. [0079]
  • FIG. 57 shows nucleic acid SEQ. ID. No.:113 and amino acid SEQ. ID. No.:114. [0080]
  • FIG. 58 shows nucleic acid SEQ. ID. No.:115 and amino acid SEQ. ID. No.:116. [0081]
  • FIG. 59 shows nucleic acid SEQ. ID. No.:117 and amino acid SEQ. ID. No.:118. [0082]
  • FIG. 60 shows nucleic acid SEQ. ID. No.:119 and amino acid SEQ. ID. No.:120. [0083]
  • FIG. 61 shows nucleic acid SEQ. ID. No.:121 and amino acid SEQ. ID. No.:122. [0084]
  • FIG. 62 shows nucleic acid SEQ. ID. No.:123 and amino acid SEQ. ID. No.:124. [0085]
  • FIG. 63 shows nucleic acid SEQ. ID. No.:125 and amino acid SEQ. ID. No.:126. [0086]
  • FIG. 64 shows nucleic acid SEQ. ID. No.:127 and amino acid SEQ. ID. No.:128. [0087]
  • FIG. 65 shows nucleic acid SEQ. ID. No.:129 and amino acid SEQ. ID. No.:130. [0088]
  • FIG. 66 shows nucleic acid SEQ. ID. No.:131 and amino acid SEQ. ID. No.:132. [0089]
  • FIG. 67 shows nucleic acid SEQ. ID. No.:133 and amino acid SEQ. ID. No.:134. [0090]
  • FIG. 68 shows nucleic acid SEQ. ID. No.:135 and amino acid SEQ. ID. No.:136. [0091]
  • FIG. 69 shows nucleic acid SEQ. ID. No.:137 and amino acid SEQ. ID. No.:138. [0092]
  • FIG. 70 shows nucleic acid SEQ. ID. No.:139 and amino acid SEQ. ID. No.:140. [0093]
  • FIG. 72 shows nucleic acid SEQ. ID. No.:143 and amino acid SEQ. ID. No.:144. [0094]
  • FIG. 73 shows nucleic acid SEQ. ID. No.:145 and amino acid SEQ. ID. No.:146. [0095]
  • FIG. 74 shows nucleic acid SEQ. ID. No.:147 and amino acid SEQ. ID. No.:148. [0096]
  • FIG. 75 shows nucleic acid SEQ. ID No.: 149 and amino acid SEQ. ID. No.: 150. [0097]
  • FIG. 76 shows nucleic acid SEQ. ID No.: 151 and amino acid SEQ. ID. No.: 152. [0098]
  • FIG. 77 shows nucleic acid SEQ. ID No.: 153 and amino acid SEQ. ID. No.: 154. [0099]
  • FIG. 78 shows nucleic acid SEQ. ID No.: 155 and amino acid SEQ. ID. No.: 156. [0100]
  • FIG. 79 shows nucleic acid SEQ. ID No.: 157 and amino acid SEQ. ID. No.: 158. [0101]
  • FIG. 80 shows nucleic acid SEQ. ID No.: 159 and amino acid SEQ. ID. No.: 160. [0102]
  • FIG. 81 shows nucleic acid SEQ. ID No.: 161 and amino acid SEQ. ID. No.: 162. [0103]
  • FIG. 82 shows nucleic acid SEQ. ID No.: 163 and amino acid SEQ. ID. No.: 164. [0104]
  • FIG. 83 shows nucleic acid SEQ. ID No.: 165 and amino acid SEQ. ID. No.: 166. [0105]
  • FIG. 84 shows nucleic acid SEQ. ID No.: 167 and amino acid SEQ. ID. No.: 168. [0106]
  • FIG. 85 shows nucleic acid SEQ. ID No.: 169 and amino acid SEQ. ID. No.: 170. [0107]
  • FIG. 86 shows nucleic acid SEQ. ID No.: 171 and amino acid SEQ. ID. No.: 172. [0108]
  • FIG. 87 shows nucleic acid SEQ. ID No.: 173 and amino acid SEQ. ID. No.: 174. [0109]
  • FIG. 88 shows nucleic acid SEQ. ID No.: 175 and amino acid SEQ. ID. No.: 176. [0110]
  • FIG. 89 shows nucleic acid SEQ. ID No.: 177 and amino acid SEQ. ID. No.: 178. [0111]
  • FIG. 90 shows nucleic acid SEQ. ID No.: 179 and amino acid SEQ. ID. No.: 180. [0112]
  • FIG. 91 shows nucleic acid SEQ. ID No.: 181 and amino acid SEQ. ID. No.: 182. [0113]
  • FIG. 92 shows nucleic acid SEQ. ID No.: 183 and amino acid SEQ. ID. No.: 184. [0114]
  • FIG. 93 shows nucleic acid SEQ. ID No.: 185 and amino acid SEQ. ID. No.: 186. [0115]
  • FIG. 94 shows nucleic acid SEQ. ID No.: 187 and amino acid SEQ. ID. No.: 188. [0116]
  • FIG. 95 shows nucleic acid SEQ. ID No.: 189 and amino acid SEQ. ID. No.: 190. [0117]
  • FIG. 96 shows nucleic acid SEQ. ID No.: 191 and amino acid SEQ. ID. No.: 192. [0118]
  • FIG. 97 shows nucleic acid SEQ. ID No.: 193 and amino acid SEQ. ID. No.: 194. [0119]
  • FIG. 98 shows nucleic acid SEQ. ID No.: 195 and amino acid SEQ. ID. No.: 196. [0120]
  • FIG. 99 shows nucleic acid SEQ. ID No.: 197 and amino acid SEQ. ID. No.: 198. [0121]
  • FIG. 100 shows nucleic acid SEQ. ID No.: 199 and amino acid SEQ. ID. No.: 200. [0122]
  • FIG. 101 shows nucleic acid SEQ. ID No.: 201 and amino acid SEQ. ID. No.: 202. [0123]
  • FIG. 102 shows nucleic acid SEQ. ID No.: 203 and amino acid SEQ. ID. No.: 204. [0124]
  • FIG. 103 shows nucleic acid SEQ. ID No.: 205 and amino acid SEQ. ID. No.: 206. [0125]
  • FIG. 104 shows nucleic acid SEQ. ID No.: 207 and amino acid SEQ. ID. No.: 208. [0126]
  • FIG. 105 shows nucleic acid SEQ. ID No.: 209 and amino acid SEQ. ID. No.: 210. [0127]
  • FIG. 106 shows nucleic acid SEQ. ID No.: 211 and amino acid SEQ. ID. No.: 212. [0128]
  • FIG. 107 shows nucleic acid SEQ. ID No.: 213 and amino acid SEQ. ID. No.: 214. [0129]
  • FIG. 108 shows nucleic acid SEQ. ID No.: 215 and amino acid SEQ. ID. No.: 216. [0130]
  • FIG. 109 shows nucleic acid SEQ. ID No.: 217 and amino acid SEQ. ID. No.: 218. [0131]
  • FIG. 110 shows nucleic acid SEQ. ID No.: 219 and amino acid SEQ. ID. No.: 220. [0132]
  • FIG. 111 shows nucleic acid SEQ. ID No.: 221 and amino acid SEQ. ID. No.: 222. [0133]
  • FIG. 112 shows nucleic acid SEQ. ID No.: 223 and amino acid SEQ. ID. No.: 224. [0134]
  • FIG. 113 shows nucleic acid SEQ. ID No.: 225 and amino acid SEQ. ID. No.: 226. [0135]
  • FIG. 114 shows nucleic acid SEQ. ID No.: 227 and amino acid SEQ. ID. No.: 228. [0136]
  • FIG. 115 shows nucleic acid SEQ. ID No.: 229 and amino acid SEQ. ID. No.: 230. [0137]
  • FIG. 116 shows nucleic acid SEQ. ID No.: 231 and amino acid SEQ. ID. No.: 232. [0138]
  • FIG. 117 shows nucleic acid SEQ. ID No.: 233 and amino acid SEQ. ID. No.: 234. [0139]
  • FIG. 118 shows nucleic acid SEQ. ID No.: 235 and amino acid SEQ. ID. No.: 236. [0140]
  • FIG. 119 shows nucleic acid SEQ. ID No.: 237 and amino acid SEQ. ID. No.: 238. [0141]
  • FIG. 120 shows nucleic acid SEQ. ID No.: 239 and amino acid SEQ. ID. No.: 240. [0142]
  • FIG. 121 shows nucleic acid SEQ. ID No.: 241 and amino acid SEQ. ID. No.: 242. [0143]
  • FIG. 122 shows nucleic acid SEQ. ID No.: 243 and amino acid SEQ. ID. No.: 244. [0144]
  • FIG. 123 shows nucleic acid SEQ. ID No.: 245 and amino acid SEQ. ID. No.: 246. [0145]
  • FIG. 124 shows nucleic acid SEQ. ID No.: 247 and amino acid SEQ. ID. No.: 248. [0146]
  • FIG. 125 shows nucleic acid SEQ. ID No.: 249 and amino acid SEQ. ID. No.: 250. [0147]
  • FIG. 126 shows nucleic acid SEQ. ID No.: 251 and amino acid SEQ. ID. No.: 252. [0148]
  • FIG. 127 shows nucleic acid SEQ. ID No.: 253 and amino acid SEQ. ID. No.: 254. [0149]
  • FIG. 128 shows nucleic acid SEQ. ID No.: 255 and amino acid SEQ. ID. No.: 256. [0150]
  • FIG. 129 shows nucleic acid SEQ. ID No.: 257 and amino acid SEQ. ID. No.: 258. [0151]
  • FIG. 130 shows nucleic acid SEQ. ID No.: 259 and amino acid SEQ. ID. No.: 260. [0152]
  • FIG. 131 shows nucleic acid SEQ. ID No.: 261 and amino acid SEQ. ID. No.: 262. [0153]
  • FIG. 132 shows nucleic acid SEQ. ID No.: 263 and amino acid SEQ. ID. No.: 264. [0154]
  • FIG. 133 shows nucleic acid SEQ. ID No.: 265 and amino acid SEQ. ID. No.: 266. [0155]
  • FIG. 134 shows nucleic acid SEQ. ID No.: 267 and amino acid SEQ. ID. No.: 268. [0156]
  • FIG. 135 shows nucleic acid SEQ. ID No.: 269 and amino acid SEQ. ID. No.: 270. [0157]
  • FIG. 136 shows nucleic acid SEQ. ID No.: 271 and amino acid SEQ. ID. No.: 272. [0158]
  • FIG. 137 shows nucleic acid SEQ. ID No.: 273 and amino acid SEQ. ID. No.: 274. [0159]
  • FIG. 138 shows nucleic acid SEQ. ID No.: 275 and amino acid SEQ. ID. No.: 276. [0160]
  • FIG. 139 shows nucleic acid SEQ. ID No.: 277 and amino acid SEQ. ID. No.: 278. [0161]
  • FIG. 140 shows nucleic acid SEQ. ID No.: 279 and amino acid SEQ. ID. No.: 280. [0162]
  • FIG. 141 shows nucleic acid SEQ. ID No.: 281 and amino acid SEQ. ID. No.: 282. [0163]
  • FIG. 142 shows nucleic acid SEQ. ID No.: 283 and amino acid SEQ. ID. No.: 284. [0164]
  • FIG. 143 shows nucleic acid SEQ. ID No.: 285 and amino acid SEQ. ID. No.: 286. [0165]
  • FIG. 144 shows nucleic acid SEQ. ID No.: 287 and amino acid SEQ. ID. No.: 288. [0166]
  • FIG. 145 shows nucleic acid SEQ. ID No.: 289 and amino acid SEQ. ID. No.: 290. [0167]
  • FIG. 146 shows nucleic acid SEQ. ID No.: 291 and amino acid SEQ. ID. No.: 292. [0168]
  • FIG. 147 shows nucleic acid SEQ. ID No.: 293 and amino acid SEQ. ID. No.: 294. [0169]
  • FIG. 148 shows nucleic acid SEQ. ID No.: 295 and amino acid SEQ. ID. No.: 296. [0170]
  • FIG. 149 shows nucleic acid SEQ. ID No.: 297 and amino acid SEQ. ID. No.: 298. [0171]
  • FIG. 151 shows a comparison of Sequence Groups. [0172]
  • FIG. 152 illustrates alignment of full length clones. [0173]
  • FIG. 153 shows a procedure used for cloning of cytochrome p450 cDNA fragments by PCR[0174]
    • DETAILED DESCRIPTION
  • Definitions [0175]
  • Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Singleton et al. (1994) Dictionary of Microbiology and Molecular Biology, second edition, John Wiley and Sons (New York) provides one of skill with a general dictionary of many of the terms used in this invention. All patents and publications referred to herein are incorporated by reference herein. For purposes of the present invention, the following terms are defined below. [0176]
  • “Enzymatic activity” is meant to include demethylation, hydroxylation, epoxidation, N-oxidation, sulfooxidation, N—, S—, and O-dealkylations, desulfation, deamination, and reduction of azo, nitro, and N-oxide groups. The term “nucleic acid” refers to a deoxyribonucleotide or ribonucleotide polymer in either single- or double-stranded form, or sense or anti-sense, and unless otherwise limited, encompasses known analogues of natural nucleotides that hybridize to nucleic acids in a manner similar to naturally occurring nucleotides. Unless otherwise indicated, a particular nucleic acid sequence includes the complementary sequence thereof. [0177]
  • The terms “operably linked”, “in operable combination”, and “in operable order” refer to functional linkage between a nucleic acid expression control sequence (such as a promoter, signal sequence, or array of transcription factor binding sites) and a second nucleic acid sequence, wherein the expression control sequence affects transcription and/or translation of the nucleic acid corresponding to the second sequence. [0178]
  • The term “recombinant” when used with reference to a cell indicates that the cell replicates a heterologous nucleic acid, expresses said nucleic acid or expresses a peptide, heterologous peptide, or protein encoded by a heterologous nucleic acid. Recombinant cells can express genes or gene fragments in either the sense or antisense form that are not found within the native (non-recombinant) form of the cell. Recombinant cells can also express genes that are found in the native form of the cell, but wherein the genes are modified and re-introduced into the cell by artificial means. [0179]
  • A “structural gene” is that portion of a gene comprising a DNA segment encoding a protein, polypeptide or a portion thereof, and excluding the 5′ sequence which drives the initiation of transcription. The structural gene may alternatively encode a nontranslatable product. The structural gene may be one which is normally found in the cell or one which is not normally found in the cell or cellular location wherein it is introduced, in which case it is termed a “heterologous gene”. A heterologous gene may be derived in whole or in part from any source known to the art, including a bacterial genome or episome, eukaryotic, nuclear or plasmid DNA, cDNA, viral DNA or chemically synthesized DNA. A structural gene may contain one or more modifications that could effect biological activity or its characteristics, the biological activity or the chemical structure of the expression product, the rate of expression or the manner of expression control. Such modifications include, but are not limited to, mutations, insertions, deletions and substitutions of one or more nucleotides. The structural gene may constitute an uninterrupted coding sequence or it may include one or more introns, bounded by the appropriate splice junctions. The structural gene may be translatable or non-translatable, including in an anti-sense orientation. The structural gene may be a composite of segments derived from a plurality of sources and from a plurality of gene sequences (naturally occurring or synthetic, where synthetic refers to DNA that is chemically synthesized). [0180]
  • “Derived from” is used to mean taken, obtained, received, traced, replicated or descended from a source (chemical and/or biological). A derivative may be produced by chemical or biological manipulation (including, but not limited to, substitution, addition, insertion, deletion, extraction, isolation, mutation and replication) of the original source. [0181]
  • “Chemically synthesized”, as related to a sequence of DNA, means that portions of the component nucleotides were assembled in vitro. Manual chemical synthesis of DNA may be accomplished using well established procedures (Caruthers, [0182] Methodology of DNA and RNA Seauencing, (1983), Weissman (ed.), Praeger Publishers, New York, Chapter 1); automated chemical synthesis can be performed using one of a number of commercially available machines.
  • Optimal alignment of sequences for comparison may be conducted by the local homology algorithm of Smith and Waterman, Adv. Appl. Math. 2:482 (1981), by the homology alignment algorithm of Needleman and Wunsch, J. Mol. Biol. 48:443 (1970), by the search for similarity method of Pearson and Lipman Proc. Natl. Acad. Sci. (U.S.A.) 85: 2444 (1988), by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, Wis.), or by inspection. [0183]
  • The NCBI Basic Local Alignment Search Tool (BLAST) (Altschul et al., 1990) is available from several sources, including the National Center for Biological Information (NCBI, Bethesda, Md.) and on the Internet, for use in connection with the sequence analysis programs blastp, blastn, blastx, tblastn and tblastx. It can be accessed at htp://www.ncbi.nlm.nih.gov/BLAST/. A description of how to determine sequence identity using this program is available at http://www.ncbi.nlm.nih.gov/BLAST/blast help.html. [0184]
  • The terms “substantial amino acid identity” or “substantial amino acid sequence identity” as applied to amino acid sequences and as used herein denote a characteristic of a polypeptide, wherein the peptide comprises a sequence that has at least 70 percent sequence identity, preferably 80 percent amino acid sequence identity, more preferably 90 percent amino acid sequence identity, and most preferably at least 99 to 100 percent sequence identity as compared to a reference group over region corresponding to the first amino acid following the cytochrome p450 motif GXRXCX(G/A) to the stop codon of the translated peptide. [0185]
  • The terms “substantial nucleic acid identity” or “substantial nucleic acid sequence identity” as applied to nucleic acid sequences and as used herein denote a characteristic of a polynucleotide sequence, wherein the polynucleotide comprises a sequence that has at least 75 percent sequence identity, preferably 81 percent amino acid sequence identity, more preferably at least 91 percent sequence identity, and most preferably at least 99 to 100 percent sequence identity as compared to a reference group over region corresponding to the first nucleic acid following the cytochrome p450 motif GXRXCX(G/A) to the stop codon of the translated peptide. [0186]
  • Another indication that nucleotide sequences are substantially identical is if two molecules hybridize to each other under stringent conditions. Stringent conditions are sequence-dependent and will be different in different circumstances. Generally, stringent conditions are selected to be about 5° C. to about 20° C., usually about 10° C. to about 15° C., lower than the thermal melting point (Tm) for the specific sequence at a defined ionic strength and pH. The Tm is the temperature (under defined ionic strength and pH) at which 50% of the target sequence hybridizes to a matched probe. Typically, stringent conditions will be those in which the salt concentration is about 0.02 molar at [0187] pH 7 and the temperature is at least about 60° C. For instance in a standard Southern hybridization procedure, stringent conditions will include an initial wash in 6×SSC at 42° C. followed by one or more additional washes in 0.2×SSC at a temperature of at least about 55° C., typically about 60° C. and often about 65° C.
  • Nucleotide sequences are also substantially identical for purposes of this invention when the polypeptides and/or proteins which they encode are substantially identical. Thus, where one nucleic acid sequence encodes essentially the same polypeptide as a second nucleic acid sequence, the two nucleic acid sequences are substantially identical, even if they would not hybridize under stringent conditions due to degeneracy permitted by the genetic code (see, Darnell et al. (1990) Molecular Cell Biology, Second Edition Scientific American Books W. H. Freeman and Company New York for an explanation of codon degeneracy and the genetic code). Protein purity or homogeneity can be indicated by a number of means well known in the art, such as polyacrylamide gel electrophoresis of a protein sample, followed by visualization upon staining. For certain purposes high resolution may be needed and HPLC or a similar means for purification may be utilized. [0188]
  • As used herein, the term “vector” is used in reference to nucleic acid molecules that transfer DNA segment(s) into a cell. A vector may act to replicate DNA and may reproduce independently in a host cell. The term “vehicle” is sometimes used interchangeably with “vector.” The term “expression vector” as used herein refers to a recombinant DNA molecule containing a desired coding sequence and appropriate nucleic acid sequences necessary for the expression of the operably linked coding sequence in a particular host organism. Nucleic acid sequences necessary for expression in prokaryotes usually include a promoter, an operator (optional), and a ribosome binding site, often along with other sequences. Eucaryotic cells are known to utilize promoters, enhancers, and termination and polyadenylation signals. [0189]
  • For the purpose of regenerating complete genetically engineered plants with roots, a nucleic acid may be inserted into plant cells, for example, by any technique such as in vivo inoculation or by any of the known in vitro tissue culture techniques to produce transformed plant cells that can be regenerated into complete plants. Thus, for example, the insertion into plant cells may be by in vitro inoculation by pathogenic or non-pathogenic [0190] A. tumefaciens. Other such tissue culture techniques may also be employed.
  • “Plant tissue” includes differentiated and undifferentiated tissues of plants, including, but not limited to, roots, shoots, leaves, pollen, seeds, tumor tissue and various forms of cells in culture, such as single cells, protoplasts, embryos and callus tissue. The plant tissue may be in planta or in organ, tissue or cell culture. [0191]
  • “Plant cell” as used herein includes plant cells in planta and plant cells and protoplasts in culture. [0192]
  • “cDNA” or “complementary DNA” generally refers to a single stranded DNA molecule with a nucleotide sequence that is complementary to an RNA molecule. cDNA is formed by the action of the enzyme reverse transcriptase on an RNA template. [0193]
  • Strategies for Obtaining Nucleic Acid Sequences [0194]
  • In accordance with the present invention, RNA was extracted from Nicotiana tissue of converter and non-converter Nicotiana lines. The extracted RNA was then used to create cDNA. Nucleic acid sequences of the present invention were then generated using two strategies. [0195]
  • In the first strategy, the poly A enriched RNA was extracted from plant tissue and cDNA was made by reverse transcription PCR. The single strand cDNA was then used to create p450 specific PCR populations using degenerate primers plus a oligo d(T) reverse primer. The primer design was based on the highly conserved motifs of p450. Examples of specific degenerate primers are set forth in FIG. 1. Sequence fragments from plasmids containing appropriate size inserts were further analyzed. These size inserts typically ranged from about 300 to about 800 nucleotides depending on which primers were used. [0196]
  • In a second strategy, a cDNA library was initially constructed. The cDNA in the plasmids was used to create p450 specific PCR populations using degenerate primers plus T7 primer on plasmid as reverse primer. As in the first strategy, sequence fragments from plasmids containing appropriate size inserts were further analyzed. [0197]
  • Nicotiana plant lines known to produce high levels of nornicotine (converter) and plant lines having undetectable levels of nornicotine may be used as starting materials. [0198]
  • Leaves can then be removed from plants and treated with ethylene to activate p450 enzymatic activities defined herein. Total RNA is extracted using techniques known in the art. cDNA fragments can then be generated using PCR (RT-PCR) with the oligo d(T) primer as described in FIG. 153. The cDNA library can then be constructed more fully described in examples herein. [0199]
  • The conserved region of p450 type enzymes can be used as a template for degenerate primers (FIG. 75). Using degenerate primers, p450 specific bands can be amplified by PCR. Bands indicative for p450 like enzymes can be identified by DNA sequencing. PCR fragments can be characterized using BLAST search, alignment or other tools to identify appropriate candidates. [0200]
  • Sequence information from identified fragments can be used to develop PCR primers. These primers in combination of plasmid primers in cDNA library were used to clone full length p450 genes. Large-scale Southern reverse analysis was conducted to examine the differential expression for all fragment clones obtained and in some cases full length clones. In this aspect of the invention, these large-scale reverse Southern assays can be conducted using labeled total cDNA's from different tissues as a probe to hybridize with cloned DNA fragments in order to screen all cloned inserts. Nonradioactive and radioactive (p[0201] 32) Northern blotting assays were also used to characterize clones p450 fragments and full length clones.
  • Peptide specific antibodies were made against several full-length clones by deriving their amino acid sequence and selecting peptide regions that were antigenic and unique relative to other clones. Rabbit antibodies were made to synthetic peptides conjugated to a carrier protein. Western blotting analyses or other immunological methods were performed on plant tissue using these antibodies. [0202]
  • Nucleic acid sequences identified as described above can be examined by using virus induced gene silencing technology (VIGS, Baulcombe, Current Opinions in Plant Biology, 1999, 2:109-113). [0203]
  • Peptide specific antibodies were made for several full-length clones by deriving their amino acid sequence and selecting peptide regions that were potentially antigenic and were unique relative to other clones. Rabbit antibodies were made to synthetic petides conjugated to a carrier protein. Western blotting analyses were perfomed using these antibodies. [0204]
  • In another aspect of the invention, interfering RNA technology (RNAi) is used to further characterize cytochrome p450 enzymatic activities in Nicotiana plants of the present invention. The following references which describe this technology are incorporated by reference herein, Smith et al., Nature, 2000, 407:319-320; Fire et al., Nature, 1998, 391:306- 311; Waterhouse et al., PNAS, 1998, 95:13959-13964; Stalberg et al., Plant Molecular Biology, 1993, 23:671- 683; Baulcombe, Current Opinions in Plant Biology, 1999, 2:109-113; and Brigneti et al., EMBO Journal, 1998, 17(22):6739-6746. Plants may be transformed using RNAi techniques, antisense techniques, or a variety of other methods described. [0205]
  • Several techniques exist for introducing foreign genetic material into plant cells, and for obtaining plants that stably maintain and express the introduced gene. Such techniques include acceleration of genetic material coated onto microparticles directly into cells (U.S. Pat. Nos. 4,945,050 to Cornell and 5,141,131 to DowElanco). Plants may be transformed using Agrobacterium technology, see U.S. Pat. No. 5,177,010 to University of Toledo, U.S. Pat. No. 5,104,310 to Texas A&M, European Patent Application 0131624B1, European Patent Applications 120516, 159418B1, European Patent Applications 120516, 159418B1 and 176,112 to Schilperoot, U.S. Pat. Nos. 5,149,645, 5,469,976, 5,464,763 and 4,940,838 and 4,693,976 to Schilperoot, European Patent Applications 116718, 290799, 320500 all to MaxPlanck, European Patent Applications 604662 and 627752 to Japan Nicotiana, European Patent Applications 0267159, and 0292435 and U.S. Pat. No. 5,231,019 all to Ciba Geigy, U.S. Pat. Nos. 5,463,174 and 4,762,785 both to Calgene, and U.S. Pat. Nos. 5,004,863 and 5,159,135 both to Agracetus. Other transformation technology includes whiskers technology, see U.S. Pat. Nos. 5,302,523 and 5,464,765 both to Zeneca. Electroporation technology has also been used to transform plants, see WO 87/06614 to Boyce Thompson Institute, U.S. Pat. Nos. 5,472,869 and 5,384,253 both to Dekalb, WO9209696 and WO9321335 both to PGS. All of these transformation patents and publications are incorporated by reference. In addition to numerous technologies for transforming plants, the type of tissue which is contacted with the foreign genes may vary as well. Such tissue would include but would not be limited to embryogenic tissue, callus tissue type I and II, hypocotyl, meristem, and the like. Almost all plant tissues may be transformed during dedifferentiation using appropriate techniques within the skill of an artisan. [0206]
  • Foreign genetic material introduced into a plant may include a selectable marker. The preference for a particular marker is at the discretion of the artisan, but any of the following selectable markers may be used along with any other gene not listed herein which could function as a selectable marker. Such selectable markers include but are not limited to aminoglycoside phosphotransferase gene of transposon Tn5 (Aph II) which encodes resistance to the antibiotics kanamycin, neomycin and G418, as well as those genes which code for resistance or tolerance to glyphosate; hygromycin; methotrexate; phosphinothricin (bar); imidazolinones, sulfonylureas and triazolopyrimidine herbicides, such as chlorosulfuron; bromoxynil, dalapon and the like. [0207]
  • In addition to a selectable marker, it may be desirous to use a reporter gene. In some instances a reporter gene may be used without a selectable marker. Reporter genes are genes which are typically not present or expressed in the recipient organism or tissue. The reporter gene typically encodes for a protein which provide for some phenotypic change or enzymatic property. Examples of such genes are provided in K. Weising et al. Ann. Rev. Genetics, 22, 421 (1988), which is incorporated herein by reference. Preferred reporter genes include without limitation glucuronidase (GUS) gene and GFP genes. [0208]
  • Once introduced into the plant tissue, the expression of the structural gene may be assayed by any means known to the art, and expression may be measured as mRNA transcribed, protein synthesized, or the amount of gene silencing that occurs (see U.S. Pat. No. 5,583,021 which is hereby incorporated by reference). Techniques are known for the in vitro culture of plant tissue, and in a number of cases, for regeneration into whole plants (EP Appln No. 88810309.0). Procedures for transferring the introduced expression complex to commercially useful cultivars are known to those skilled in the art. [0209]
  • Once plant cells expressing the desired level of p450 enzyme are obtained, plant tissues and whole plants can be regenerated therefrom using methods and techniques well-known in the art. The regenerated plants are then reproduced by conventional means and the introduced genes can be transferred to other strains and cultivars by conventional plant breeding techniques. [0210]
  • The following examples illustrate methods for carrying out the invention and should be understood to be illustrative of, but not limiting upon, the scope of the invention which is defined in the appended claims. [0211]
    • EXAMPLES Example 1 Development of Plant Tissue and Ethylene Treatment
  • Plant Growth [0212]
  • Plants were seeded in pots and grown in a greenhouse for 4 weeks. The 4 week old seedlings were transplanted into individual pots and grown in the greenhouse for 2 months. The plants were watered 2 times a day with water containing 150 ppm NPK fertilizer during growth. The expanded green leaves were detached from plants to do the ethylene treatment described below. [0213]
  • Cell Line 78379 [0214]
  • Tobacco line 78379, which is a burley tobacco line released by the University of Kentucky was used as a source of plant material. One hundred plants were cultured as standard in the art of growing tobacco and transplanted and tagged with a distinctive number (1-100). Fertilization and field management were conducted as recommended. [0215]
  • Three quarters of the 100 plants converted between 20 and 100% of the nicotine to nornicotine. One quarter of the 100 plants converted less than 5% of the nicotine to nornicotine. [0216] Plant number 87 had the least conversion (2%) while plant number 21 had 100% conversion. Plants converting less than 3% were classified as non-converters. Self-pollinated seed of plant number 87 and plant number 21, as well as crossed (21×87 and 87×21) seeds were made to study genetic and phenotypic differences. Plants from selfed 21 were converters, and 99% of selfs from 87 were non-converters. The other 1% of the plants from 87 showed low conversion (5-15%). Plants from reciprocal crosses were all converters.
  • Cell Line 4407 [0217]
  • Nicotiana line 4407, which is a burley line was used as a source of plant material. Uniform and representative plants (100) were selected and tagged. Of the 100 [0218] plants 97 were non-converters and three were converters. Plant number 56 had the least amount of conversion (1.2%) and plant number 58 had the highest level of conversion (96%). Self-pollenated seeds and crossed seeds were made with these two plants.
  • Plants from selfed-58 segregated with 3:1 converter to non-converter ratio. Plants 58-33 and 58-25, were identified as homozygous converter and nonconverter plant lines, respectively. The stable conversion of 58-33 was confirmed by analysis of its progenies of next generation. [0219]
  • Cell Line PBLB01 [0220]
  • PBLB01 is a burley line developed by ProfiGen, Inc. and was used as a source of plant material. The converter plant was selected from foundation seeds of PBLB01. [0221]
  • Ethylene Treatment Procedures [0222]
  • Green leaves were detached from 2-3 month greenhouse grown plants and sprayed with 0.3% ethylene solution (Prep brand Ethephon (Rhone-Poulenc)). Each sprayed leaf was hung in a curing rack equipped with humidifier and covered with plastic. During the treatment, the sample leaves were periodically sprayed with the ethylene solution. Approximately 24-48 hour post ethylene treatment, leaves were collected for RNA extraction. Another sub-sample was taken for metabolic constituent analysis to determine the concentration of leaf metabolites and more specific constituents of interest such as a variety of alkaloids. [0223]
  • As an example, alkaloids analysis could be performed as follows. Samples (0.1 g) were shaken at 150 rpm with 0.5 ml 2N NaOH, and a 5 ml extraction solution which contained quinoline as an internal standard and methyl t-butyl ether. Samples were analyzed on a HP 6890 GC equipped with a FID detector. A temperature of 250° C. was used for the detector and injector. An HP column (30 m-0.32 nm-1 m) consisting of fused silica crosslinked with 5% phenol and 95% methyl silicon was used at a temperature gradient of 110-185° C. at 10° C. per minute. The column was operated at 100° C. with a flow rate of 1.7 cm[0224] 3min−1 with a split ratio of 40:1 with a 2 l injection volume using helium as the carrier gas.
    • Example 2 RNA Isolation
  • For RNA extractions, middle leaves from 2 month old greenhouse grown plants were treated with ethylene as described. The 0 and 24-48 hours samples were used for RNA extraction. In some cases, leaf samples under the senescence process were taken from the [0225] plants 10 days post flower-head removal. These samples were also used for extraction. Total RNA was isolated using Rneasy Plant Mini Kit® (Qiagen, Inc., Valencia, Calif.) following manufacturer's protocol.
  • The tissue sample was ground under liquid nitrogen to a fine powder using a DEPC treated mortar and pestle. Approximately 100 milligrams of ground tissue were transferred to a sterile 1.5 ml eppendorf tube. This sample tube was placed in liquid nitrogen until all samples were collected. Then, 450μ-l of Buffer RLT as provided in the kit (with the addition of Mercaptoethanol) was added to each individual tube. The sample was vortexed vigorously and incubated at 56° C. for 3 minutes. The lysate was then, applied to the QIAshredder™ spin column sitting in a 2-ml collection tube, and centrifuged for 2 minutes at maximum speed. The flow through was collected and 0.5 volume of ethanol was added to the cleared lysate. The sample is mixed well and transferred to an Rneasy® mini spin column sitting in a 2 ml collection tube. The sample was centrifuged for 1 minute at 10,000 rpm. Next, 700 μl of buffer RW1 was pipetted onto the Rneasy® column and centrifuged for 1 minute at 10,000 rpm. Buffer RPE was pipetted onto the Rneasy® column in a new collection tube and centrifuged for 1 minute at 10,000 rpm. Buffer RPE was again, added to the Rneasy® spin column and centrifuged for 2 minutes at maximum speed to dry the membrane. To eliminate any ethanol carry over, the membrane was placed in a separate collection tube and centrifuged for an additional 1 minute at maximum speed. The Rneasy® column was transferred into a new 1.5 ml collection tube, and 40 μl of Rnase-free water was pipetted directly onto the Rneasy® membrane. This final elute tube was centrifuged for 1 minute at 10,000 rpm. Quality and quantity of total RNA was analyzed by denatured formaldehyde gel and spectrophotometer. [0226]
  • Poly(A)RNA was isolated using Oligotex™ poly A+ RNA purification kit (Qiagen Inc.) following manufacture's protocol. About 200 μg total RNA in 250 μl maximum volume was used. A volume of 250 μl of Buffer OBB and 15 μl of oligotex™ suspension was added to the 250 μl of total RNA. The contents were mixed thoroughly by pipetting and incubated for 3 minutes at 70° C. on a heating block. The sample was then, placed at room temperature for approximately 20 minutes. The oligotex:mRNA complex was pelleted by centrifugation for 2 minutes at maximum speed. All but 50 μl of the supernatant was removed from the microcentrifuge tube. The sample was treated further by OBB buffer. The oligotex:mRNA pellet was resuspended in 400 μl of Buffer OW2 by vortexing. This mix was transferred onto a small spin column placed in a new tube and centrifuged for 1 minute at maximum speed. The spin column was transferred to a new tube and an additional 400 μl of Buffer OW2 was added to the column. The tube was then centrifuged for 1 minute at maximum speed. The spin column was transferred to a final 1.5 ml microcentrifuge tube. The sample was eluted with 60 ul of hot (70° C.) Buffer OEB. Poly A product was analyzed by denatured formaldehyde gels and spectrophotometric analysis. [0227]
    • Example 3 Reverse Transcription-PCR
  • First strand cDNA was produced using SuperScript reverse transcriptase following manufacturer's protocol (Invitrogen, Carlsbad, Calif.). The poly A+ enriched RNA/oligo dT primer mix consisted of less than 5 μg of total RNA, 1 μl of 10 mM DNTP mix, 1 μl of Oligo d(T)[0228] 12-18 (0.5 μg/ul), and up to 10 μl of DEPC-treated water. Each sample was incubated at 65° C. for 5 minutes, then placed on ice for at least 1 minute. A reaction mixture was prepared by adding each of the following components in order: 2 μl 10× RT buffer, 4 μl of 25 mM MgCl2, 2 μl of 0.1 M DTT, and 1 μl of RNase OUT Recombinant RNase Inhibitor. An addition of 9 μl of reaction mixture was pipetted to each RNA/primer mixture and gently mixed. It was incubated at 42° C. for 2 minutes and 1 μl of Super Script II™ RT was added to each tube. The tube was incubated for 50 minutes at 42° C. The reaction was terminated at 70° C. for 15 minutes and chilled on ice. The sample was collected by centrifugation and 1 μl of RNase H was added to each tube and incubated for 20 minutes at 37° C. The second PCR was carried out with 200 pmoles of forward primer (degenerate primers as in FIG. 75, SEQ.ID Nos. 149-156) and 100 pmoles reverse primer (mix of 18 nt oligo d(T) followed by 1 random base).
  • Reaction conditions were 94° C. for 2 minutes and then performed 40 cycles of PCR at 94° C. for 1 minute, 45° to 60° C. for 2 minutes, 72° C. for 3 minutes with a 72° C. extension for an extra 10 min. [0229]
  • Ten microliters of the amplified sample were analyzed by electrophoresis using a 1% agarose gel. The correct size fragments were purified from agarose gel. [0230]
    • Example 4 Generation of PCR Fragment Populations
  • PCR fragments from Example 3 were ligated into a pGEM-T® Easy Vector (Promega, Madison, Wis.) following manufacturer's instructions. The ligated product was transformed into JM109 competent cells and plated on LB media plates for blue/white selection. Colonies were selected and grown in a 96 well plate with 1.2 ml of LB media overnight at 37° C. Frozen stock was generated for all selected colonies. Plasmid DNA from plates were purified using Beckman's Biomeck 2000 miniprep robotics with Wizard SV Miniprep® kit (Promega). Plasmid DNA was eluted with 100 μl water and stored in a 96 well plate. Plasmids were digested by EcoR1 and were analyzed using 1% agarose gel to confirm the DNA quantity and size of inserts. The plasmids containing a 400-600 bp insert were sequenced using an CEQ 2000 sequencer (Beckman, Fullerton, Calif.). The sequences were aligned with GenBank database by BLAST search. The p450 related fragments were identified and further analyzed. Alternatively, p450 fragments were isolated from substraction libraries. These fragments were also analyzed as described above. [0231]
    • Example 5 Construction of CDNA Library
  • A cDNA library was constructed by preparing total RNA from ethylene treated leaves as follows. First, total RNA was extracted from ethylene treated leaves of tobacco line 58-33 using a modified acid phenol and chloroform extraction protocol. Protocol was modified to use one gram of tissue that was ground and subsequently vortexed in 5 ml of extraction buffer (100 mM Tris-HCl, pH 8.5; 200 mM NaCl; 10 mM EDTA; 0.5% SDS) to which 5 ml phenol (pHS.5) and 5 ml chloroform was added. The extracted sample was centrifuged and the supernatant was saved. This extraction step was repeated 2-3 more times until the supernatant appeared clear. Approximately 5 ml of chloroform was added to remove trace amounts of phenol. RNA was precipitated from the combined supernatant fractions by adding a 3-fold volume of ETOH and 1/10 volume of 3M NaOAc (pH5.2) and storing at −20° C. for 1 hour. After transferring to a Corex glass container the RNA fraction was centrifuged at 9,000 RPM for 45 minutes at 4° C. The pellet was washed with 70% ethanol and spun for 5 minutes at 9,000 RPM at 4° C. After drying the pellet, the pelleted RNA was dissolved in 0.5 ml RNase free water. The pelleted RNA was dissolved in 0.5 ml RNase free water. The quality and quantity of total RNA was analyzed by denatured formaldehyde gel and spectrophotometer, respectively. [0232]
  • The resultant total RNA was isolated for poly A+ RNA using an Oligo(dT) cellulose protocol (Invitrogen) and Microcentrifuge spin columns (Invitrogen) by the following protocol. Approximately twenty mg of total RNA was subjected to twice purification to obtain high quality poly A+ RNA. Poly A+ RNA product was analyzed by performing denatured formaldehyde gel and subsequent RT-PCR of known full-length genes to ensure high quality of mRNA. [0233]
  • Next, poly A+ RNA was used as template to produce a CDNA library employing cDNA synthesis kit, ZAP-cDNA® synthesis kit, and ZAP-cDNA® Gigapack® III gold cloning kit (Stratagene, La Jolla, Calif.). The method involved following the manufacture's protocol as specified. Approximately 8 μg of poly A+ RNA was used to construct cDNA library. Analysis of the primary library revealed about 2.5×10[0234] 6-1×107 pfu. A quality background test of the library was completed by complementation assays using IPTG and X-gal, where recombinant plaques was expressed at more than 100-fold above the background reaction.
  • A more quantitative analysis of the library by random PCR showed that average size of insert cDNA was approximately 1.2 kb. The method used a two-step PCR method as followed. For the first step, reverse primers were designed based on the preliminary sequence information obtained from p450 fragments. The designed reverse primers and T3 (forward) primers were used amplify corresponding genes from the cDNA library. PCR reactions were subjected to agarose electrophoresis and the corresponding bands of high molecular weight were excised, purified, cloned and sequenced. In the second step, new primers designed from 5′UTR or the start coding region of p450 as the forward primers together with the reverse primers (designed from 3′UTR of p450) were used in the subsequent PCR to obtain full-length p450 clones. [0235]
  • The p450 fragments were generated by PCR amplification from the constructed cDNA library as described in Example 3 with the exception of the reverse primer. The T7 primer located on the plasmid downstream of cDNA inserts (see FIG. 75) was used as a reverse primer. PCR fragments were isolated, cloned and sequenced as described in Example 4. [0236]
  • Full-length p450 genes were isolated by PCR method from constructed cDNA library. Gene specific reverse primers (designed from the downstream sequence of p450 fragments) and a forward primer (T3 on library plasmid) were used to clone the full length genes. PCR fragments were isolated, cloned and sequenced. If necessary, second step PCR was applied. In the second step, new forward primers designed from 5′UTR of cloned p450s together with the reverse primers designed from 3′UTR of p450 clones were used in the subsequent PCR reactions to obtain full-length p450 clones. The clones were subsequently sequenced. [0237]
    • Example 6 Characterization of Cloned Fragments—Reverse Southern Blotting Analysis
  • Nonradioactive large scale reverse southern blotting assays were performed on all p450 clones identified in above examples to detect the differential expression. It was observed that the level of expression among different p450 clusters was very different. Further real time detection was conducted on those with high expression. [0238]
  • Nonradioactive Southern blotting procedures were conducted as follows. [0239]
  • 1) Total RNA was extracted from ethylene treated and nontreated converter (58-33) and nonconverter (58-25) leaves using the Qiagen Rnaeasy kit as described in Example 2. [0240]
  • 2) Probe was produced by biotin-tail labeling a single strand cDNA derived from poly A+ enriched RNA generated in above step. This labeled single strand cDNA was generated by RT-PCR of the converter and nonconverter total RNA (Invitrogen) as described in Example 3 with the exception of using biotinalyted oligo dT as a primer (Promega). These were used as a probe to hybridize with cloned DNA. [0241]
  • 3) Plasmid DNA was digested with restriction enzyme EcoR1 and run on agarose gels. Gels were simultaneously dried and transferred to two nylon membranes (Biodyne B®). One membrane was hybridized with converter probe and the other with nonconverter probe. Membranes were UV-crosslinked (auto crosslink setting, 254 nm, Stratagene, Stratalinker) before hybridization. [0242]
  • Alternatively, the inserts were PCR amplified from each plasmid using the sequences located on both arms of p-GEM plasmid, T3 and SP6, as primers. The PCR products were analyzed by running on a 96 well Ready-to-run agarose gels. The confirmed inserts were dotted on two nylon membranes. One membrane was hybridized with converter probe and the other with nonconverter probe. [0243]
  • 4) The membranes were hybridized and washed following manufacture's instruction with the modification of washing stringency (Enzo MaxSence™ kit, Enzo Diagnostics, Inc, Farmingdale, N.Y.). The membranes were prehybridized with hybridization buffer (2×SSC buffered formamide, containing detergent and hybridization enhancers) at 42° C. for 30 min and hybridized with 10 μl denatured probe overnight at 42° C. The membranes then were washed in 1× [0244] hybridization wash buffer 1 time at room temperature for 10 min and 4 times at 68° C. for 15 min. The membranes were ready for the detection.
  • 5) The washed membranes were detected by alkaline phosphatase labeling followed by NBT/BCIP colometric detection as described in manufacture's detection procedure (Enzo Diagnostics, Inc.). The membranes were blocked for one hour at room temperature with 1× blocking solution, washed 3 times with 1× detection reagents for 10 min, washed 2 times with 1× predevelopment reaction buffer for 5 min and then developed the blots in developing solution for 30-45 min until the dots appear. All reagents were provided by manufacture (Enzo Diagnostics, Inc). In Addition, large scale reverse Southern assay was also performed using KPL southern hybridization and detection kit™ following manfacturer's instruction(KPL, Gaithersburg, Md.). [0245]
    • Example 7 Characterization of Clones—Northern Blot Analysis
  • Alternative to Southern Blot analysis, some membranes were hybridized and detected as described in the example of Northern blotting assays. Northern Hybridization was used to detect mRNA differentially expressed in Nicotiana as follows. [0246]
  • A random priming method was used to prepare probes from cloned p450 (Megaprime™ DNA Labelling Systems, Amersham Biosciences). [0247]
  • The following components were mixed: 25 ng denatured DNA template; 4 ul of each unlabeled dTTP, dGTP and dCTP; 5 ul of reaction buffer; P[0248] 32-labelled DATP and 2 ul of Klenow I; and H2O, to bring the reaction to 50 μl. The mixture was incubated in 37° C. for 1-4 hours, then stopped with 2 μl of 0.5 M EDTA. The probe was denatured by incubating at 95° C. for 5 minutes before use.
  • RNA samples were prepared from ethylene treated and non-treated fresh leaves of several pairs of tobacco lines. In some cases poly A+ enriched RNA was used. Approximately 15 μg total RNA or 1.8 μg mRNA (methods of RNA and mRNA extraction as described in Example 5) were brought to equal volume with DEPC H[0249] 2O (5-10 μl). The same volume of loading buffer (1× MOPS; 18.5% Formaldehyde; 50% Formamide; 4% Ficoll400; Bromophenolblue) and 0.5 μl EtBr (0.5 μg/μl) were added. The samples were subsequently denatured in preparation for separation of the RNA by electrophoresis.
  • Samples were subjected to electrophoresis on a formaldehyde gel (1% Agarose, 1×MOPS, 0.6 M Formaldehyde) with 1×MOP buffer (0.4 M Morpholinopropanesulfonic acid; 0.1 M Na-acetate-3× H2O; 10 mM EDTA; adjust to pH 7.2 with NaOH). RNA was transferred to a Hybond-N+ membrane (Nylon, Amersham Pharmacia Biotech) by capillary method in 10×SSC buffer (1.5 M NaCl; 0.15 M Na-citrate) for 24 hours. Membranes with RNA samples were UV-crosslinked (auto crosslink setting, 254 nm, Stratagene, Stratalinker) before hybridization. [0250]
  • The membrane was prehybridized for 1-4 hours at 42° C. with 5-10 ml prehybridization buffer (5×SSC; 50% Formamide; 5× Denhardt's-solution; 1% SDS; 100 μg/ml heat-denatured sheared non- homologous DNA). Old prehybridization buffer was discarded, and new prehybridization buffer and probe were added. The hybridization was carried out over night at 42° C. The membrane was washed for 15 minutes with 2×SSC at room temperature, followed by a wash with 2×SSC. [0251]
  • A major focus of the invention was the discovery of novel genes that may be induced as a result of ethylene treatment or play a key role in tobacco leaf quality and constituents. As illustrated in the table below, Northern blots and reverse Southern Blot were useful in determining which genes were induced by ethylene treatment relative to non-induced plants. Interestingly, not all fragments were affected similarly in the converter and nonconverter. The cytochrome p450 fragments of interest were partially sequenced to determine their structural relatedness. This information was used to subsequently isolate and characterize full length gene clones of interest. [0252]
    Induced mRNA Expression
    Ethylene Treatment
    Fragments Converter
    D56-AC7 (SEQ ID No: 35) +
    D56-AG11 (SEQ ID No: 31) +
    D56-AC12 (SEQ ID No: 45) +
    D70A-AB5 (SEQ ID No: 95) +
    D73-AC9 (SEQ ID No: 43) +
    D70A-AA12 (SEQ ID No: 131) +
    D73A-AG3 (SEQ ID No: 129) +
    D34-52 (SEQ ID No: 61) +
    D56-AG6 (SEQ ID No: 51) +
  • Northern analysis was performed using full length clones on tobacco tissue obtained from converter and nonconverter burley lines that were induced by ethylene treatment. The purpose was to identify those full length clones that showed elevated expression in ethylene induced converter lines relative to ethylene induced converter lines relative to ethylene induced nonconverter burley lines. By so doing, the functionality relationship of full length clones may be determined by comparing biochemical differences in leaf constituents between converter and nonconverter lines. As shown in table below, six clones showed significantly higher expression, as denoted by ++ and +++, in converter ethylene treated tissue than that of nonconverter treated tissue, denoted by +. All of these clones showed little or no expression in converter and nonconverter lines that were not ethylene treated. [0253]
    Full Length
    Clones Converter Nonconverter
    D101-BA2 ++ +
    D207-AA5 ++ +
    D208-AC8 +++ +
    D237-AD1 ++ +
    D89-AB1 ++ +
    D90A-BB3 ++ +
    • Example 8 Immunodetection of P450S Encoded by the Cloned Genes
  • Peptide regions corresponding to 20-22 amino acids in length from three p450 clones were selected for 1) having lower or no homology to other clones and 2) having good hydrophilicity and antigenicity. The amino acid sequences of the peptide regions selected from the respective p450 clones are listed below. The synthesized peptides were conjugated with KHL and then injected into rabbits. Antisera were collected 2 and 4 weeks after the 4[0254] th injection (Alpha Diagnostic Intl. Inc. San Antonio, Tex.).
    D234-AD1 DIDGSKSKLVKAHRKIDEILG
    D90a-BB3 RDAFREKETFDENDVEELNY
    D89-AB1 FKNNGDEDRHFSQKLGDLADKY
  • Antisera were examined for crossreactivity to target proteins from tobacco plant tissue by Western Blot analysis. Crude protein extracts were obtained from ethylene treated (0 to 40 hours) middle leaves of converter and nonconverter lines. Protein concentrations of the extracts were determined using RC DC Protein Assay Kit (BIO-RAD) following the manufacturer's protocol. [0255]
  • Two micrograms of protein were loaded onto each lane and the proteins separated on 10%-20% gradient gels using the Laemmli SDS-PAGE system. The proteins were transferred from gels to PROTRAN® Nitrocellulose Transfer Membranes (Schleicher & Schuell) with the Trans-Blot® Semi-Dry cell (BIO-RAD). Target p450 proteins were detected and visualized with the ECL Advances Western Blotting Detection Kit (Amersham Biosciences). Primary antibodies against the synthetic-KLH conjugates were made in rabbits. Secondary antibody against rabbit IgG, coupled with peroxidase, was purchased from Sigma. Both primary and secondary antibodies were used at 1:1000 dilutions. Antibodies showed strong reactivity to a single band on the Western Blots indicating that the antisera were monospecific to the target peptide of interest. Antisera were also crossreactive with synthetic peptides conjuated to KLH. [0256]
    • Example 9 Nucleic Acid Identity and Structure Relatedness of Isolated Nucleic Acid Fragments
  • Over 100 cloned p450 fragments were sequenced in conjunction with Northern blot analysis to determine their structural relatedness. The approach used utilized forward primers based either of two common p450 motifs located near the carboxyl-terminus of the p450 genes. The forward primers corresponded to cytochrome p450 motifs FXPERF or GRRXCP(A/G) as denoted in FIG. 1. The reverse primers used standard primers from either the plasmid, SP6 or T7 located on both arms of pGEM™ plasmid, or a poly A tail. The protocol used is described below. [0257]
  • Spectrophotometry was used to estimate the concentration of starting double stranded DNA following the manufacturer's protocol (Beckman Coulter). The template was diluted with water to the appropriate concentration, denatured by heating at 95° C. for 2 minutes, and subsequently placed on ice. The sequencing reaction was prepared on ice using 0.5 to 10 μl of denatured DNA template, 2 μl of 1.6 pmole of the forward primer, 8 μl of DTCS Quick Start Master Mix and the total volume brought to 20 μl with water. The thermocycling program consisted of 30 cycles of the follow cycle: 96° C. for 20 seconds, 50° C. for 20 seconds, and 60° C. for 4 minutes followed by holding at 4° C. [0258]
  • The sequence was stopped by adding 5 μl of stop buffer (equal volume of 3M NaOAc and 100 mM EDTA and 1 μl of 20 mg/ml glycogen). The sample was precipitated with 60 μl of cold 95% ethanol and centrifuged at 6000 g for 6 minutes. Ethanol was discarded. The pellet was 2 washes with 200 μl of cold 70% ethanol. After the pellet was dry, 40 μl of SLS solution was added and the pellet was resuspended. A layer of mineral oil was over laid. The sample was then, placed on the CEQ 8000 Automated Sequencer for further analysis. [0259]
  • In order to verify nucleic acid sequences, nucleic acid sequence was re-sequenced in both directions using forward primers to the FXPERF or GRRXCP(A/G) region of the p450 gene or reverse primers to either the plasmid or poly A tail. All sequencing was performed at least twice in both directions. [0260]
  • The nucleic acid sequences of cytochrome p450 fragments were compared to each other from the coding region corresponding to the first nucleic acid after the region encoding the GRRXCP(A/G) motif through to the stop codon. [0261]
  • This region was selected as an indicator of genetic diversity among p450 proteins. A large number of genetically distinct p450 genes, in excess of 70 genes, were observed, similar to that of other plant species. Upon comparison of nucleic acid sequences, it was found that the genes could be placed into distinct sequences groups based on their sequence identity. It was found that the best unique grouping of p450 members was determined to be those sequences with 75% nucleic acid identity or greater (shown in Table I). Reducing the percentage identity resulted in significantly larger groups. A preferred grouping was observed for those sequences with 81% nucleic acid identity or greater, a more [0262] preferred grouping 91% nucleic acid identity or greater, and a most preferred grouping for those sequences 99% nucleic acid identity of greater. Most of the groups contained at least two members and frequently three or more members. Others were not repeatedly discovered suggesting that approach taken was able to isolated both low and high expressing mRNA in the tissue used.
  • Based on 75% nucleic acid identity or greater, two cytochrome p450 groups were found to contain nucleic acid sequence identity to previously tobacco cytochrome genes that genetically distinct from that within the group. [0263] Group 23, showed nucleic acid identity, within the parameters used for Table I, to prior GenBank sequences of GI:1171579 (CAA64635) and GI:14423327 (or AAK62346) by Czernic et al and Ralston et al, respectively. GI:1171579 had nucleic acid identity to Group 23 members ranging 96.9% to 99.5% identity to members of Group 23 while GI:14423327 ranged 95.4% to 96.9% identity to this group. The members of Group 31 had nucleic acid identity ranging from 76.7% to 97.8% identity to the GenBank reported sequence of GI:14423319 (AAK62342) by Ralston et al. None of the other p450 identity groups of Table 1 contained parameter identity, as used in Table 1, to Nicotiana p450s genes reported by Ralston et al, Czernic et al., Wang et al or LaRosa and Smigocki.
  • As shown in FIG. 76, consensus sequence with appropriate nucleic acid degenerate probes could be derived for group to preferentially identify and isolate additional members of each group from Nicotiana plants. [0264]
    TABLE I
    Nicotiana p450 Nucleic
    Acid Sequence Identity Groups
    GROUP FRAGMENTS
     1 D58-BG7 (SEQ ID No.:1), D58-AB1 (SEQ ID No.:3); D58-BE4 (SEQ ID No.:7)
     2 D56-AH7 (SEQ ID No.:9); D13a-5 (SEQ ID No.:11)
     3 D56-AG10 (SEQ ID No.:13); D35-33 (SEQ ID No.:15);
    D34-62 (SEQ ID No.:17)
     4 D56-AA7 (SEQ ID No.:19); D56-AE1 (SEQ ID No.:21);
    185-BD3 (SEQ ID No.:143)
     5 D35-BB7 (SEQ ID No.:23); D177-BA7 (SEQ ID No.:25);
    D56A-AB6 (SEQ ID No.:27); D144-AE2 (SEQ ID No.:29)
     6 D56-AG11 (SEQ ID No.:31); D179-AA1 (SEQ ID No.:33)
     7 D56-AC7 (SEQ ID No.:35); D144-AD1 (SEQ ID No.:37)
     8 D144-AB5 (SEQ ID No.:39)
     9 D181-AB5 (SEQ ID No.:41); D73-Ac9 (SEQ ID No.:43)
    10 D56-AC12 (SEQ ID No.:45)
    11 D58-AB9 (SEQ ID No.:47); D56-AG9 (SEQ ID No.:49);
    D56-AG6 (SEQ ID No.:51); D35-BG11 (SEQ ID No.:53); D35-42
    (SEQ ID No.:55); D35-BA3 (SEQ ID No.:57); D34-57 (SEQ ID
    No.:59); D34-52 (SEQ ID No.:61); D34-25 (SEQ ID No.:63)
    12 D56-AD10 (SEQ ID No.:65)
    13 56-AA11 (SEQ ID No.:67)
    14 D177-BD5 (SEQ ID No.:69); D177-BD7 (SEQ ID No.:83)
    15 D56A-AG10 (SEQ ID No.:71); D58-BC5 (SEQ ID No.:73);
    D58-AD12 (SEQ ID No.:75)
    16 D56-AC11 (SEQ ID No.:77); D35-39 (SEQ ID No.:79);
    D58-BH4 (SEQ ID No.:81); D56-AD6 (SEQ ID No.:87)
    17 D73A-AD6 (SEQ ID No.:89); D70A-BA11 (SEQ ID No.:91)
    18 D70A-AB5 (SEQ ID No.:95); D70A-AA8 (SEQ ID No.:97)
    19 D70A-AB8 (SEQ ID No.:99); D70A-BH2 (SEQ ID No.:101);
    D70A-AA4 (SEQ ID No.:103)
    20 D70A-BA1 (SEQ ID No.:105); D70A-BA9 (SEQ ID No.:107)
    21 D70A-BD4 (SEQ ID No.:109)
    22 D181-AC5 (SEQ ID No.:111); D144-AH1 (SEQ ID No.:113);
    D34-65 (SEQ ID No.:115)
    23 D35-BG2 (SEQ ID No.:117)
    24 D73A-AH7 (SEQ ID No.:119)
    25 D58-AA1 (SEQ ID No.:121); D185-BC1 (SEQ ID No.:133);
    D185-BG2 (SEQ ID No.:135)
    26 D73-AE10 (SEQ ID No.:123)
    27 D56-AC12 (SEQ ID No.:125)
    28 D177-BF7 (SEQ ID No.:127); D185-BE1 (SEQ ID No.:137);
    D185-BD2 (SEQ ID No.:139)
    29 D73A-AG3 (SEQ ID No.:129)
    30 D70A-AA12 (SEQ ID No.:131); D176-BF2 (SEQ ID No.:85)
    31 D176-BC3 (SEQ ID No.:145)
    32 D176-BB3 (SEQ ID No.:147)
  • D186-AH4 (SEQ ID No.:5) [0265]
    • Example 10 Related Amino Acid Sequence Identity of Isolated Nucleic Acid Fragments
  • The amino acid sequences of nucleic acid sequences obtained for cytochrome p450 fragments from Example 8 were deduced. The deduced region corresponded to the amino acid immediately after the GXRXCP(A/G) sequence motif to the end of the carboxyl-terminus, or stop codon. Upon comparison of sequence identity of the fragments, a unique grouping was observed for those sequences with 70% amino acid identity or greater. A preferred grouping was observed for those sequences is with 80% amino acid identity or greater, more preferred with 90% amino acid identity or greater, and a most preferred grouping for those [0266] sequences 99% amino acid identity of greater. The groups and corresponding amino acid sequences of group members are shown in FIG. 2. Several of the unique nucleic acid sequences were found to have complete amino acid identity to other fragments and therefore only one member with the identical amino acid was reported.
  • The amino acid identity for [0267] Group 19 of Table II corresponded to three distinct groups based on their nucleic acid sequences. The amino acid sequences of each group member and their identity is shown in FIG. 77. The amino acid differences are appropriated marked.
  • At least one member of each amino acid identity group was selected for gene cloning and functional studies using plants. In addition, group members that are differentially affected by ethylene treatment or other biological differences as assessed by Northern and Southern analysis were selected for gene cloning and functional studies. To assist in gene cloning, expression studies and whole plant evaluations, peptide specific antibodies will be prepared on sequence identity and differential sequence. [0268]
    TABLE II
    Nicotiana p450 Amino Acid
    Sequence Identity Groups
    GROUP FRAGMENTS
     1 D58-BG7 (SEQ ID No.:2), D58-AB1 (SEQ ID No.:4)
     2 D58-BE4 (SEQ ID No.:8)
     3 D56-AH7 (SEQ ID No.:10); D13a-5 (SEQ ID No.:12)
     4 D56-AG10 (SEQ ID No.:14); D34-62 (SEQ ID No.:18)
     5 D56-AA7 (SEQ ID No.:20); D56-AE1 (SEQ ID No.:22); 185-
    BD3 (SEQ ID No.:144)
     6 D35-BB7 (SEQ ID No.:24); D177-BA7 (SEQ ID No.:26);
    D56A-AB6 (SEQ ID No.:28); D144-AE2 (SEQ ID No.:30)
     7 D56-AG11 (SEQ ID No.:32); D179-AA1 (SEQ ID No.:34)
     8 D56-AC7 (SEQ ID No.:36); D144-AD1 (SEQ ID No.:38)
     9 D144-AB5 (SEQ ID No.:40)
    10 D181-AB5 (SEQ ID No.:42); D73-Ac9 (SEQ ID No.:44)
    11 D56-AC12 (SEQ ID No.:46)
    12 D58-AB9 (SEQ ID No.:48); D56-AG9 (SEQ ID No.:50); D56-
    AG6 (SEQ ID No.:52); D35-BG11 (SEQ ID No.:54); D35-42 (SEQ
    ID No.:56); D35-BA3 (SEQ ID No.:58); D34-57 (SEQ ID
    No.:60); D34-52 (SEQ ID No.:62)
    13 D56AD10 (SEQ ID No.:66)
    14 56-AA11 (SEQ ID No.:68)
    15 D177-BD5 (SEQ ID No.:70); D177-BD7 (SEQ ID No.:84)
    16 D56A-AG10 (SEQ ID No.:72); D58-BC5 (SEQ ID No.:74);
    D58-AD12 (SEQ ID No.:76)
    17 D56-AC11 (SEQ ID No.:78); D56-AD6 (SEQ ID No.:88)
    18 D73A-AD6 (SEQ ID No.90:)
    19 D70A-AB5 (SEQ ID No.:96); D70A-AB8 (SEQ ID No.:100);
    D70A-BH2 (SEQ ID No.:102); D70A-AA4 (SEQ ID No.:104); D70A-
    BA1 (SEQ ID No.:106); D70A-BA9 (SEQ ID No.:108)
    20 D70A-BD4 (SEQ ID No.:110)
    21 D181-AC5 (SEQ ID No.:112); D144-AH1 (SEQ ID No.:114);
    D34-65 (SEQ ID No.:116)
    22 D35-BG2 (SEQ ID No.:118)
    23 D73A-AH7 (SEQ ID No.:120)
    24 D58-AA1 (SEQ ID No.:122); D185-BC1 (SEQ ID No.:134);
    D185-BG2 (SEQ ID No.:136)
    25 D73-AE10 (SEQ ID No.:124)
    26 D56-AC12 (SEQ ID No.:126)
    27 D177-BF7 (SEQ ID No.:128); 185-BD2 (SEQ ID No.:140)
    28 D73A-AG3 (SEQ ID No.:130)
    29 D70A-AA12 (SEQ ID No.:132); D176-BF2 (SEQ ID No.:86)
    30 D176-BC3 (SEQ ID No.:146)
    31 D176-BB3 (SEQ ID No.:148)
    32 D186-AH4 (SEQ ID No.:6)
    • Example 11 Related Amino Acid Sequence Identity of Full Length Clones
  • The nucleic acid sequence of full length Nicotiana genes cloned in Example 5 were deduced for their entire amino acid sequence. Cytochrome p450 genes were identified by the presence of three conserved p450 domain motifs, which corresponded to UXXRXXZ, PXRFXF or GXRXC at the carboxyl-terminus where U is E or K, X is any amino acid and Z is P, T, S or M. It was also noted that two of the clones appeared nearly complete but lacked the appropriate stop codon, D130-AA1 and D101-BA2, however but both contained all three p450 cytochrome domains. All p450 genes were characterized for amino acid identity using a BLAST program comparing their full length sequences to each other and to known tobacco genes. The program used the NCBI special BLAST tool (Align two sequences (b12seq), http://www.ncbi.nlm.nih.gov/blast/b12seq/b12.html). Two sequences were aligned under BLASTN without filter for nucleic acid sequences and BLASTP for amino acid sequences. Based on their percentage amino acid identity, each sequence was grouped into identity groups where the grouping contained members that shared at least 85% identity with another member. A preferred grouping was observed for those sequences with 90% amino acid identity or greater, a more preferred grouping had 95% amino acid identity or greater, and a most preferred grouping had those [0269] sequences 99% amino acid identity or greater. Using these criteria, 25 unique groups were identified and are depicted in Table III.
  • Within the parameters used for Table III for amino acid identity, three groups were found to contain greater than 85% or greater identity to known tobacco genes. Members of [0270] Group 5 had up to 96% amino acid identity for full length sequences to prior GenBank sequences of GI:14423327 (or AAK62346) by Ralston et al. Group 23 had up to 93% amino acid identity to GI:14423328 (or AAK62347) by Ralston et al. and Group 24 had 92% identity to GI:14423318 (or AAK62343) by Ralston et al.
    TABLE III
    Amino Acid Sequence Identity
    Groups of Full Length Nicotiana p450 Genes
     1 D208-AD9 (SEQ. ID. No. 224); D120-AH4
    (SEQ. ID. No. 180); D121-AA8 (SEQ. ID.
    No. 182), D122-AF10 (SEQ. ID. No. 184);
    D103-AH3 (SEQ. ID. No. 222);
    D208-AC8 (SEQ.
    ID. No. 218); D-235-ABI (SEQ. ID.
    No. 246)
     2 D244-AD4 (SEQ. ID. No. 250); D244-AB6
    (SEQ. ID. No. 274) ; D285-AA8; D285-
    AB9; D268-AE2 (SEQ. ID. No. 270)
     3 D100A-AC3 (SEQ. ID. No. 168);
    D100A-BE2
     4 D205-BE9 (SEQ. ID. No. 276); D205-BG9
    (SEQ. ID. No. 202); D205-AH4 (SEQ. ID.
    No. 294)
     5 D259-AB9 (SEQ. ID. No. 260) ; D257-AE4
    (SEQ. ID. No. 268) ; D147-AD3 (SEQ. ID.
    No. 194)
     6 D249-AE8 (SEQ. ID. No. 256); D-248-AA6
    (SEQ. ID. No. 254)
     7 D233-AG7 (SEQ. ID. No. 266; D224-BD11
    (SEQ. ID. No. 240); DAF10
     8 D105-AD6 (SEQ. ID. No. 172); D215-AB5
    (SEQ. ID. No. 220); D135-AE1 (SEQ. ID.
    No. 190)
     9 D87A-AF3 (SEQ. ID. No. 216), D210-BD4
    (SEQ. ID. No. 262)
    10 D89-AB1 (SEQ. ID. No. 150); D89-AD2
    (SEQ. ID. No. 152); 163-AG11
    (SEQ. ID. No. 198); 163-AF12
    (SEQ. ID. No. 196)
    11 D267-AF10 (SEQ. ID. No. 296); D96-AC2
    (SEQ. ID. No. 160); D96-AB6 (SEQ. ID.
    No. 158); D207-AA5 (SEQ. ID. No. 204);
    D207-AB4 (SEQ. ID. No. 206); D207-AC4
    (SEQ. ID. No. 208)
    12 D98-AG1 (SEQ. ID. No. 164); D98-AA1
    (SEQ. ID. No. 162)
    13 D209-AA12 (SEQ. ID. No. 212); D209-
    AA11; D209-AH10
    (SEQ. ID. No. 214); D209-AH12 (SEQ.
    ID. No. 232);
    D90a-BB3 (SEQ. ID. No. 154)
    14 D129-AD10 (SEQ. ID. No. 188);
    D104A-AE8
    (SEQ. ID. No. 170)
    15 D228-AH8 (SEQ. ID. No. 244);
    D228-AD7 (SEQ. ID. No. 241), D250-
    AC11 (SEQ. ID. No. 258); D247-AH1
    (SEQ. ID. No. 252)
    16 D128-AB7 (SEQ. ID. No. 186) ;
    D243-AA2 (SEQ. ID. No. 248); D125-AF11
    (SEQ. ID. No. 228)
    17 D284-AH5 (SEQ. ID. No. 298); D110-AF12
    (SEQ. ID. No. 176)
    18 D221-BB8 (SEQ. ID. No. 234)
    19 D222-BH4 (SEQ. ID. No. 236)
    20 D134-AE11 (SEQ. ID. No. 230)
    21 D109-AH8 (SEQ. ID. No. 174)
    22 D136-AF4 (SEQ. ID. No. 278)
    23 D237-AD1 (SEQ. ID. No. 226)
    24 D112-AA5 (SEQ. ID. No. 178)
    25 D283-AC1 (SEQ. ID. No. 272)
  • The full length genes were further grouped based on the highly conversed amino acid homology between UXXRXXZ p450 domain and GXRXC p450 domain near the end the carboxyl-terminus. As shown in FIG. 3, individual clones were aligned for their sequence homology between the conserved domains relative to each other and placed in distinct identity groups. In several cases, although the nucleic acid sequence of the clone was unique, the amino acid sequence for the region was identical. The preferred grouping was observed for those sequences with 90% amino acid identity or greater, a more preferred group had 95% amino acid identity or greater, and a most preferred grouping had those [0271] sequences 99% amino acid identity of greater. The final grouping was similar to that based on the percent identity for the entire amino acid sequence of the clones except for Group 17 (of Table III) which was divided into two distinct groups.
  • Within the parameters used for amino acid identity in Table IV, three groups were found to contain 90% or greater identity to known tobacco genes. Members of [0272] Group 5 had up to 93.4% amino acid identity for full length sequences to prior GenBank sequences of GI:14423326 (AAK62346) by Ralston et al. Group 23 had up to 91.8% amino acid identity to GI:14423328 (or AAK62347) by Ralston et al. and Group 24 had 98.8% identity to GI:14423318 (or AAK62342) by Ralston et al.
    TABLE IV
    Amino Acid Sequence Identity Groups of Regions
    between Conserved Domains of Nicotiana p450 Genes
     1 1 D208-AD9 (SEQ. ID. No. 224); D120-AH4
    (SEQ. ID. No. 180); D121-AA8 (SEQ. ID. No.
    182), D122-AF10 (SEQ. ID. No. 184);
    D103-AH3 (SEQ. ID. No. 222); D208-AC8
    (SEQ. ID. No. 218); D-235-ABI (SEQ. ID.
    No. 246)
     2 D244-AD4 (SEQ. ID. No. 250); D244-AB6
    (SEQ. ID. No. 274); D285-AA8; D285-AB9;
    D268-AE2 (SEQ. ID. No. 270)
     3 D100A-AC3 (SEQ. ID. No. 168); D100A-BE2
     4 D205-BE9 (SEQ. ID. No. 276); D205-BG9
    (SEQ. ID. No. 202); D205-AH4 (SEQ. ID.
    No. 294)
     5 D259-AB9 (SEQ. ID. No. 260) ; D257-AE4
    (SEQ. ID. No. 268); D147-AD3 (SEQ. ID.
    No. 194)
     6 D249-AE8 (SEQ. ID. No. 256); D-248-AA6
    (SEQ. ID. No. 254)
     7 D233-AG7 (SEQ. ID. No. 266; D224-BD11
    (SEQ. ID. No. 240) ; DAF10
     8 D105-AD6 (SEQ. ID. No. 172); D215-AB5
    (SEQ. ID. No. 220) ; D135-AE1 (SEQ.
    ID. No. 190)
     9 D87A-AF3 (SEQ. ID. No. 216), D210-BD4
    (SEQ. ID. No. 262)
    10 D89-AB1 (SEQ. ID. No. 150); D89-AD2
    (SEQ. ID. No. 152);
    163-AG11 (SEQ. ID. No. 198); 163-AF12
    (SEQ. ID. No. 196)
    11 D267-AF10 (SEQ. ID. No. 296); D96-AC2
    (SEQ. ID. No. 160); D96-AB6 (SEQ. ID. No.
    158); D207-AA5 (SEQ. ID. No. 204);
    D207-AB4 (SEQ. ID. No. 206); D207-AC4
    (SEQ. ID. No. 208)
    12 D98-AG1 (SEQ. ID. No. 164); D98-AA1
    (SEQ. ID. No. 162)
    13 D209-AA12 (SEQ. ID. No. 212); D209-AA11;
    D209-AH10
    (SEQ. ID. No. 214); D209-AH12
    (SEQ. ID. No. 232); D90a-BB3 (SEQ. ID.
    No. 154)
    14 D129-AD10 (SEQ. ID. No. 188); D104A-AE8
    (SEQ. ID. No. 170)
    15 D228-AH8 (SEQ. ID. No. 244); D228-AD7
    (SEQ. ID. No. 241), D250-AC11 (SEQ. ID.
    No. 258); D247-AH1 (SEQ. ID. No. 252)
    16 D128-AB7 (SEQ. ID. No. 186) ;
    D243-AA2 (SEQ. ID. No. 248); D125-AF11
    (SEQ. ID. No. 228)
    17 D284-AH5 (SEQ. ID. No. 298); D110-AF12
    (SEQ. ID. No. 176)
    18 D221-BB8 (SEQ. ID. No. 234)
    19 D222-BH4 (SEQ. ID. No. 236)
    20 D134-AE11 (SEQ. ID. No. 230)
    21 D109-AH8 (SEQ. ID. No. 174)
    22 D136-AF4 (SEQ. ID. No. 278)
    23 D237-AD1 (SEQ. ID. No. 226)
    24 D112-AA5 (SEQ. ID. No. 178)
    25 D283-AC1 (SEQ. ID. No. 272)
    26 D110-AF12 (SEQ. ID. No. 176)
    • Example 12 Nicotiana Cytochrome P450 Clones Lacking One or more of the Tobacco Cytochrome P450 Specific Domains
  • Four clones had high nucleic acid homology, ranging 90% to 99% nucleic acid homology, to other tobacco cytochrome genes reported in Table III. The four clones included D136-AD5, D138-AD12, D243-AB3 and D250-AC11. However, due to a nucleotide frameshift these genes did not contain one or more of three C-terminus cytochrome p450 domains and were excluded from identity groups presented in Table III or Table IV. [0273]
  • The amino acid identity of one clone, D95-AG1, did not contain the third domain, GXRXC, used to group p450 tobacco genes in Table III or Table IV. The nucleic acid homology of this clone had low homology to other tobacco cytochrome genes. This clone represents a novel and different group of cytochrome p450 genes in Nicotiana. [0274]
    • Example 13 Use of Nicotiana Cytochrome P450 Fragments and Clones in Altered Regulartion of Tobacco Properties
  • The use of tobacco p450 nucleic acid fragments or whole genes are useful in identifying and selecting those plants that have altered tobacco phenotypes or tobacco constituents and, more importantly, altered metabolites. Transgenic tobacco plants are generated by a variety of transformation systems that incorporate nucleic acid fragments or full length genes, selected from those reported herein, in orientations for either down-regulation, for example anti-sense orientation, or over-expression for example, sense orienation. For over-expression to full length genes, any nucleic acid sequence that encodes the entire or a functional part or amino acide sequence of the full-length genes described in this invention are desired that are effective for increasing the expression of a certain enzyme and thus resulting in phenotypic effect within Nicotiana. Nicotiana lines that are homozygous lines are obtained through a series of backcrossing and assessed for phenotypic changes including, but not limited to, analysis of endogenous p450 RNA, transcripts, p450 expressed peptides and concentrations of plant metabolites using techniques commonly avaiable to one having ordinary skill in the art. [0275]
  • The changes exhibited in the tobacco plans provide information on the functional role of the selected gene of interest or are of a utility as a preffered Nicotiana plant species. [0276]
  • Numerous modifications and variations in practice of the invention are expected to occur to those skilled in the art upon consideration of the foregoing detailed description of the invention. Consequently, such modifications and variations are intended to be included within the scope of the following claims. [0277]
  • 1 298 1 207 DNA NICOTIANATABACUM 1 gcacaacttg ctatcaactt ggtcacatct atgttgggtc atttgttgca tcattttaca 60 tgggctccgg ccccgggggt taacccggag gatattgact tggaggagag ccctggaaca 120 gtaacttaca tgaaaaatcc aatacaagct attccaactc caagattgcc tgcacacttg 180 tatggacgtg tgccagtgga tatgtaa 207 2 68 PRT NICOTIANATABACUM 2 Ala Gln Leu Ala Ile Asn Leu Val Thr Ser Met Leu Gly His Leu Leu 1 5 10 15 His His Phe Thr Trp Ala Pro Ala Pro Gly Val Asn Pro Glu Asp Ile 20 25 30 Asp Leu Glu Glu Ser Pro Gly Thr Val Thr Tyr Met Lys Asn Pro Ile 35 40 45 Gln Ala Ile Pro Thr Pro Arg Leu Pro Ala His Leu Tyr Gly Arg Val 50 55 60 Pro Val Asp Met 65 3 207 DNA NICOTIANATABACUM 3 gcacaacttg ctatcaactt ggtcacatct atgttgggtc atttgttgca tcattttacg 60 tgggctccgc ccccgggggt taacccggag aatattgact tggaggagag ccctggaaca 120 gtaacttaca tgaaaaatcc aatacaagct attcctactc caagattgcc tgcacacttg 180 tatggacgtg tgccagtgga tatgtaa 207 4 68 PRT NICOTIANATABACUM 4 Ala Gln Leu Ala Ile Asn Leu Val Thr Ser Met Leu Gly His Leu Leu 1 5 10 15 His His Phe Thr Trp Ala Pro Pro Pro Gly Val Asn Pro Glu Asn Ile 20 25 30 Asp Leu Glu Glu Ser Pro Gly Thr Val Thr Tyr Met Lys Asn Pro Ile 35 40 45 Gln Ala Ile Pro Thr Pro Arg Leu Pro Ala His Leu Tyr Gly Arg Val 50 55 60 Pro Val Asp Met 65 5 183 DNA NICOTIANATABACUM 5 atgaattatt cattgcaagt ggaacacctt tcaattgctc atatgatcca aggtttcagt 60 tttgcaacta cgaccaatga gcctttggat atgaaacaag gtgtgggttt aactttacca 120 aagaagactg atgttgaagt gctaattaca cctcgccttc ctcctacgct ttatcaatat 180 taa 183 6 60 PRT NICOTIANATABACUM 6 Met Asn Tyr Ser Leu Gln Val Glu His Leu Ser Ile Ala His Met Ile 1 5 10 15 Gln Gly Phe Ser Phe Ala Thr Thr Thr Asn Glu Pro Leu Asp Met Lys 20 25 30 Gln Gly Val Gly Leu Thr Leu Pro Lys Lys Thr Asp Val Glu Val Leu 35 40 45 Ile Thr Pro Arg Leu Pro Pro Thr Leu Tyr Gln Tyr 50 55 60 7 132 DNA NICOTIANATABACUM 7 gcacaacttg ctatcaactt ggtcacatct atgttgggtc atttgttcat cattttacat 60 gggctccggc cccgggggtt aacccggagg atattgactt ggaggagagc cctggaacag 120 taacttacat ga 132 8 40 PRT NICOTIANATABACUM 8 Ala Gln Leu Ala Ile Asn Leu Val Thr Ser Met Leu Gly His Leu Phe 1 5 10 15 Ile Ile Leu His Gly Leu Arg Pro Arg Gly Leu Thr Arg Arg Ile Leu 20 25 30 Thr Trp Arg Arg Ala Leu Glu Gln 35 40 9 189 DNA NICOTIANATABACUM 9 gaaggattgg ctgttcgaat ggttgccttg tcattgggat gtattattca atgttttgat 60 tggcaacgaa tcggcgaaga attggttgat atgactgaag gaactggact tactttgcct 120 aaagctcaac ctttggtggc caagtgtagc ccacgaccta aaatggctaa tcttctctct 180 cagatttga 189 10 62 PRT NICOTIANATABACUM 10 Glu Gly Leu Ala Val Arg Met Val Ala Leu Ser Leu Gly Cys Ile Ile 1 5 10 15 Gln Cys Phe Asp Trp Gln Arg Ile Gly Glu Glu Leu Val Asp Met Thr 20 25 30 Glu Gly Thr Gly Leu Thr Leu Pro Lys Ala Gln Pro Leu Val Ala Lys 35 40 45 Cys Ser Pro Arg Pro Lys Met Ala Asn Leu Leu Ser Gln Ile 50 55 60 11 189 DNA NICOTIANATABACUM 11 gaaggattgg ctattcgaat ggttgcattg tcattgggat gtattattca atgctttgat 60 tggcaacgac ttggggaagg attggttgat aagactgaag gaactggact tactttgcct 120 aaagctcaac ctttagtggc caagtgtagc ccacgaccta taatggctaa tcttctttct 180 cagatttga 189 12 62 PRT NICOTIANATABACUM 12 Glu Gly Leu Ala Ile Arg Met Val Ala Leu Ser Leu Gly Cys Ile Ile 1 5 10 15 Gln Cys Phe Asp Trp Gln Arg Leu Gly Glu Gly Leu Val Asp Lys Thr 20 25 30 Glu Gly Thr Gly Leu Thr Leu Pro Lys Ala Gln Pro Leu Val Ala Lys 35 40 45 Cys Ser Pro Arg Pro Ile Met Ala Asn Leu Leu Ser Gln Ile 50 55 60 13 183 DNA NICOTIANATABACUM 13 ataggttttg cgactttagt gacacatctg acttttggtc gcttgcttca aggttttgat 60 tttagtaagc catcaaacac gccaattgac atgacagaag gcgtaggcgt tactttgcct 120 aaggttaatc aagttgaagt tctaattacc cctcgtttac cttctaagct ttatttattt 180 tga 183 14 60 PRT NICOTIANATABACUM 14 Ile Gly Phe Ala Thr Leu Val Thr His Leu Thr Phe Gly Arg Leu Leu 1 5 10 15 Gln Gly Phe Asp Phe Ser Lys Pro Ser Asn Thr Pro Ile Asp Met Thr 20 25 30 Glu Gly Val Gly Val Thr Leu Pro Lys Val Asn Gln Val Glu Val Leu 35 40 45 Ile Thr Pro Arg Leu Pro Ser Lys Leu Tyr Leu Phe 50 55 60 15 178 DNA NICOTIANATABACUM 15 ataggctttg cgactttagt gacacatctg acttttggtc gcttgcttca aggttttgat 60 tttagtaagc catcaaacac gccaattgac atgacagaag gcgtaggcgt tactttgcct 120 aaggttaatc aagttgaagt tctaattacc cctcgtttac cttctaagct ttatttat 178 16 59 PRT NICOTIANATABACUM 16 Ile Gly Phe Ala Thr Leu Val Thr His Leu Thr Phe Gly Arg Leu Leu 1 5 10 15 Gln Gly Phe Asp Phe Ser Lys Pro Ser Asn Thr Pro Ile Asp Met Thr 20 25 30 Glu Gly Val Gly Val Thr Leu Pro Lys Val Asn Gln Val Glu Val Leu 35 40 45 Ile Thr Pro Arg Leu Pro Ser Lys Leu Tyr Leu 50 55 17 183 DNA NICOTIANATABACUM 17 ataaattttg cgactttagt gacacatctg acttttggtc gcttgcttca aggttttgat 60 tttagtacgc catcaaacac gccaatagac atgacagaag gcgtaggcgt tactttgcct 120 aaggtaaatc aagtggaagt tctaattagc cctcgtttac cttctaagct ttatgtattc 180 tga 183 18 60 PRT NICOTIANATABACUM 18 Ile Asn Phe Ala Thr Leu Val Thr His Leu Thr Phe Gly Arg Leu Leu 1 5 10 15 Gln Gly Phe Asp Phe Ser Thr Pro Ser Asn Thr Pro Ile Asp Met Thr 20 25 30 Glu Gly Val Gly Val Thr Leu Pro Lys Val Asn Gln Val Glu Val Leu 35 40 45 Ile Ser Pro Arg Leu Pro Ser Lys Leu Tyr Val Phe 50 55 60 19 171 DNA NICOTIANATABACUM 19 attatacttg cattgccaat tcttggcatc actttgggac gtttggttca gaactttgag 60 ctgttgcctc ctccaggcca gtcgaagctc gacaccacag agaaaggtgg acagttcagt 120 ctccacattt tgaagcattc caccattgtg ttgaaaccaa ggtctttctg a 171 20 56 PRT NICOTIANATABACUM 20 Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Leu Gly Arg Leu Val 1 5 10 15 Gln Asn Phe Glu Leu Leu Pro Pro Pro Gly Gln Ser Lys Leu Asp Thr 20 25 30 Thr Glu Lys Gly Gly Gln Phe Ser Leu His Ile Leu Lys His Ser Thr 35 40 45 Ile Val Leu Lys Pro Arg Ser Phe 50 55 21 171 DNA NICOTIANATABACUM 21 attatacttg cattgccaat tcttggcatt actttgggac gtttggttca gaactttgag 60 ctgttgcctc ctccaggcca gtcgaagctc gacaccacag agaaaggtgg acagttcagt 120 ctccatattt tgaagcattc caccattgtg ttgaaaccaa ggtcttgctg a 171 22 56 PRT NICOTIANATABACUM 22 Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Leu Gly Arg Leu Val 1 5 10 15 Gln Asn Phe Glu Leu Leu Pro Pro Pro Gly Gln Ser Lys Leu Asp Thr 20 25 30 Thr Glu Lys Gly Gly Gln Phe Ser Leu His Ile Leu Lys His Ser Thr 35 40 45 Ile Val Leu Lys Pro Arg Ser Cys 50 55 23 172 DNA NICOTIANATABACUM 23 tattgcactt ggggttgcat caatggaact tgcattgtca aatcttcttt atgcatttga 60 ttgggagtta ccttttggaa tgaaaaaaga agacattgac acaaacgcca ggcctggaat 120 taccatgcat aagaaaaacg aactttatct tatccctaaa aattatctat ag 172 24 63 PRT NICOTIANATABACUM 24 Ile Ala Leu Gly Val Ala Ser Met Glu Leu Ala Leu Ser Asn Leu Leu 1 5 10 15 Tyr Ala Phe Asp Trp Glu Leu Pro Phe Gly Met Lys Lys Glu Asp Ile 20 25 30 Asp Thr Asn Ala Arg Pro Gly Ile Thr Met His Lys Lys Asn Glu Leu 35 40 45 Tyr Leu Ile Pro Lys Asn Tyr Leu Pro Ser Lys Leu Tyr Leu Phe 50 55 60 25 171 DNA NICOTIANATABACUM 25 attgcacttg gggttgcatc catggaactt gctttgtcaa atcttcttta tgcatttgat 60 tgggagttac cttacggagt gaaaaaagaa aacattgaca caaatgtcag gcctggaatt 120 accatgcata agaaaaacga actttgcctt atccctagaa attatctata g 171 26 56 PRT NICOTIANATABACUM 26 Ile Ala Leu Gly Val Ala Ser Met Glu Leu Ala Leu Ser Asn Leu Leu 1 5 10 15 Tyr Ala Phe Asp Trp Glu Leu Pro Tyr Gly Val Lys Lys Glu Asn Ile 20 25 30 Asp Thr Asn Val Arg Pro Gly Ile Thr Met His Lys Lys Asn Glu Leu 35 40 45 Cys Leu Ile Pro Arg Asn Tyr Leu 50 55 27 174 DNA NICOTIANATABACUM 27 ggtattgcac ttggggttgc atccatggaa cttgctttgt caaatcttct ttatgcattt 60 gattgggagt tgccttatgg agtgaaaaaa gaagacatcg acacaaacgt taggcctgga 120 attgccatgc acaagaaaaa cgaactttgc cttgtcccaa aaaattattt ataa 174 28 56 PRT NICOTIANATABACUM 28 Ile Ala Leu Gly Val Ala Ser Met Glu Leu Ala Leu Ser Asn Leu Leu 1 5 10 15 Tyr Ala Phe Asp Trp Glu Leu Pro Tyr Gly Val Lys Lys Glu Asp Ile 20 25 30 Asp Thr Asn Val Arg Pro Gly Ile Ala Met His Lys Lys Asn Glu Leu 35 40 45 Cys Leu Val Pro Lys Asn Tyr Leu 50 55 29 201 DNA NICOTIANATABACUM 29 attgcacttg gggttgcatc catggaactt gctttgtcaa atcttcttta tgcatttgat 60 tgggagttgc cttatggagt gaaaaaagaa gacatcgaca caaacgttag gcctggaatt 120 gccatgcaca agaaaaacga actttgcctt gtcccaaaaa aattatttat aaattatatt 180 gggacgtgga tctcatgcta g 201 30 66 PRT NICOTIANATABACUM 30 Ile Ala Leu Gly Val Ala Ser Met Glu Leu Ala Leu Ser Asn Leu Leu 1 5 10 15 Tyr Ala Phe Asp Trp Glu Leu Pro Tyr Gly Val Lys Lys Glu Asp Ile 20 25 30 Asp Thr Asn Val Arg Pro Gly Ile Ala Met His Lys Lys Asn Glu Leu 35 40 45 Cys Leu Val Pro Lys Lys Leu Phe Ile Asn Tyr Ile Gly Thr Trp Ile 50 55 60 Ser Cys 65 31 183 DNA NICOTIANATABACUM 31 atttcgtttg gtttagctaa tgcttatttg ccattggctc aattacttta tcactttgat 60 tgggaactcc ccactggaat caaaccaagc gacttggact tgactgagtt ggttggagta 120 actgccgcta gaaaaagtga cctttacttg gttgcgactc cttatcaacc tcctcaaaac 180 tga 183 32 60 PRT NICOTIANATABACUM 32 Ile Ser Phe Gly Leu Ala Asn Ala Tyr Leu Pro Leu Ala Gln Leu Leu 1 5 10 15 Tyr His Phe Asp Trp Glu Leu Pro Thr Gly Ile Lys Pro Ser Asp Leu 20 25 30 Asp Leu Thr Glu Leu Val Gly Val Thr Ala Ala Arg Lys Ser Asp Leu 35 40 45 Tyr Leu Val Ala Thr Pro Tyr Gln Pro Pro Gln Asn 50 55 60 33 183 DNA NICOTIANATABACUM 33 atttcgtttg gcttagctaa tgcttatttg ccattggctc aattactata tcacttcgat 60 tggaaactcc ctgctggaat cgaaccaagc gacttggact tgactgagtt ggttggagta 120 actgccgcta gaaaaagtga cctttacttg gttgcgactc cttatcaacc tcctcaaaag 180 tga 183 34 60 PRT NICOTIANATABACUM 34 Ile Ser Phe Gly Leu Ala Asn Ala Tyr Leu Pro Leu Ala Gln Leu Leu 1 5 10 15 Tyr His Phe Asp Trp Lys Leu Pro Ala Gly Ile Glu Pro Ser Asp Leu 20 25 30 Asp Leu Thr Glu Leu Val Gly Val Thr Ala Ala Arg Lys Ser Asp Leu 35 40 45 Tyr Leu Val Ala Thr Pro Tyr Gln Pro Pro Gln Lys 50 55 60 35 220 DNA NICOTIANATABACUM 35 atgctatttg gtttagctaa tgttggacaa cctttagctc agttacttta tcacttcgat 60 tggaaactcc ctaatggaca aagtcatgag aatttcgaca tgactgagtc acctggaatt 120 tctgctacaa gaaaggatga tcttgttttg attgccactc cttatgattc ttattaattc 180 cagtctatat catctatatg tactcaataa ttgtatggga 220 36 58 PRT NICOTIANATABACUM 36 Met Leu Phe Gly Leu Ala Asn Val Gly Gln Pro Leu Ala Gln Leu Leu 1 5 10 15 Tyr His Phe Asp Trp Lys Leu Pro Asn Gly Gln Ser His Glu Asn Phe 20 25 30 Asp Met Thr Glu Ser Pro Gly Ile Ser Ala Thr Arg Lys Asp Asp Leu 35 40 45 Val Leu Ile Ala Thr Pro Tyr Asp Ser Tyr 50 55 37 174 DNA NICOTIANATABACUM 37 atgctatttg gtttagctaa tgttggacaa cctttagctc agttacttta tcacttcgat 60 tggaaactcc ctaatggaca aactcaccaa aatttcgaca tgactgagtc acctggaatt 120 tctgctacaa gaaaggatga tcttattttg attgccactc ctgctcattc ttga 174 38 57 PRT NICOTIANATABACUM 38 Met Leu Phe Gly Leu Ala Asn Val Gly Gln Pro Leu Ala Gln Leu Leu 1 5 10 15 Tyr His Phe Asp Trp Lys Leu Pro Asn Gly Gln Thr His Gln Asn Phe 20 25 30 Asp Met Thr Glu Ser Pro Gly Ile Ser Ala Thr Arg Lys Asp Asp Leu 35 40 45 Ile Leu Ile Ala Thr Pro Ala His Ser 50 55 39 177 DNA NICOTIANATABACUM 39 ttattattcg gtttagttaa tgtaggacat cctttagctc aattgcttta tcacttcgat 60 tggaagactc ttcctgggat aagttcagat agtttcgaca tgactgaaac agatggagta 120 actgccggaa gaaaggatga tctttgttta attgctactc cttttggtct caattaa 177 40 58 PRT NICOTIANATABACUM 40 Leu Leu Phe Gly Leu Val Asn Val Gly His Pro Leu Ala Gln Leu Leu 1 5 10 15 Tyr His Phe Asp Trp Lys Thr Leu Pro Gly Ile Ser Ser Asp Ser Phe 20 25 30 Asp Met Thr Glu Thr Asp Gly Val Thr Ala Gly Arg Lys Asp Asp Leu 35 40 45 Cys Leu Ile Ala Thr Pro Phe Gly Leu Asn 50 55 41 180 DNA NICOTIANATABACUM 41 atgtcgtttg gtttagttaa cactgggcat cctttagctc agttgctcta tttctttgac 60 tggaaattcc ctcataaggt taatgcagct gattttcaca ctactgaaac aagtagagtt 120 tttgcagcaa gcaaagatga cctctacttg attccaacaa atcacatgga gcaagagtag 180 42 59 PRT NICOTIANATABACUM 42 Met Ser Phe Gly Leu Val Asn Thr Gly His Pro Leu Ala Gln Leu Leu 1 5 10 15 Tyr Phe Phe Asp Trp Lys Phe Pro His Lys Val Asn Ala Ala Asp Phe 20 25 30 His Thr Thr Glu Thr Ser Arg Val Phe Ala Ala Ser Lys Asp Asp Leu 35 40 45 Tyr Leu Ile Pro Thr Asn His Met Glu Gln Glu 50 55 43 180 DNA NICOTIANATABACUM 43 atgtcgtttg gtttagttaa cacagggcat cctttagccc agttgctcta ttgctttgac 60 tggaaactcc ctgacaaggt taatgcaaat gattttcgca ctactgaaac aagtagagtt 120 tttgcagcaa gcaaagatga cctctacttg attcccacaa atcacaggga gcaagaatag 180 44 59 PRT NICOTIANATABACUM 44 Met Ser Phe Gly Leu Val Asn Thr Gly His Pro Leu Ala Gln Leu Leu 1 5 10 15 Tyr Cys Phe Asp Trp Lys Leu Pro Asp Lys Val Asn Ala Asn Asp Phe 20 25 30 Arg Thr Thr Glu Thr Ser Arg Val Phe Ala Ala Ser Lys Asp Asp Leu 35 40 45 Tyr Leu Ile Pro Thr Asn His Arg Glu Gln Glu 50 55 45 183 DNA NICOTIANATABACUM 45 atgcaatttg gtttggctct tgttactctg ccattggctc atttgcttca caattttgat 60 tggaaacttc ccgaaggaat taatgcaagg gatttggaca tgacagaggc aaatgggata 120 tctgctagaa gagaaaaaga tctttacttg attgctactc cttatgtatc acctcttgat 180 taa 183 46 60 PRT NICOTIANATABACUM 46 Met Gln Phe Gly Leu Ala Leu Val Thr Leu Pro Leu Ala His Leu Leu 1 5 10 15 His Asn Phe Asp Trp Lys Leu Pro Glu Gly Ile Asn Ala Arg Asp Leu 20 25 30 Asp Met Thr Glu Ala Asn Gly Ile Ser Ala Arg Arg Glu Lys Asp Leu 35 40 45 Tyr Leu Ile Ala Thr Pro Tyr Val Ser Pro Leu Asp 50 55 60 47 177 DNA NICOTIANATABACUM 47 atgacttatg cattgcaagt ggaacaccta acaatggcac atttgatcca gggtttcaat 60 tacagaactc caactgatga gcccttggat atgaaagaag gtgcaggcat aactatacgt 120 aaggtaaatc ctgtgaaagt gataattacg cctcgcttgg cacctgagct ttattaa 177 48 58 PRT NICOTIANATABACUM 48 Met Thr Tyr Ala Leu Gln Val Glu His Leu Thr Met Ala His Leu Ile 1 5 10 15 Gln Gly Phe Asn Tyr Arg Thr Pro Thr Asp Glu Pro Leu Asp Met Lys 20 25 30 Glu Gly Ala Gly Ile Thr Ile Arg Lys Val Asn Pro Val Lys Val Ile 35 40 45 Ile Thr Pro Arg Leu Ala Pro Glu Leu Tyr 50 55 49 177 DNA NICOTIANATABACUM 49 atgacttatg cattgcaagt ggaacaccta acaatggcac atttaatcca gggtttcaat 60 tacaaaactc caaatgacga ggccttggat atgaaggaag gtgcaggcat aactatacgt 120 aaggtaaatc ctgtggaact gataatagcg cctcgcctgg cacctgagct ttattaa 177 50 58 PRT NICOTIANATABACUM 50 Met Thr Tyr Ala Leu Gln Val Glu His Leu Thr Met Ala His Leu Ile 1 5 10 15 Gln Gly Phe Asn Tyr Lys Thr Pro Asn Asp Glu Ala Leu Asp Met Lys 20 25 30 Glu Gly Ala Gly Ile Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile 35 40 45 Ile Ala Pro Arg Leu Ala Pro Glu Leu Tyr 50 55 51 177 DNA NICOTIANATABACUM 51 atgacttatg cattgcaagt ggaacaccta acaatggcac atttaatcca gggtttcaat 60 tacaaaactc caaatgacga ggccttggat atgaaggaag gtgcaggcat aacaatacgt 120 aaggtaaatc cagtggaatt gataataacg cctcgcttgg cacctgagct ttactaa 177 52 58 PRT NICOTIANATABACUM 52 Met Thr Tyr Ala Leu Gln Val Glu His Leu Thr Met Ala His Leu Ile 1 5 10 15 Gln Gly Phe Asn Tyr Lys Thr Pro Asn Asp Glu Ala Leu Asp Met Lys 20 25 30 Glu Gly Ala Gly Ile Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile 35 40 45 Ile Thr Pro Arg Leu Ala Pro Glu Leu Tyr 50 55 53 177 DNA NICOTIANATABACUM 53 atgacttatg cattgcaagt ggaacactta acaatggcac atttgatcca aggtttcaat 60 tacagaactc caaatgacga gcccttggat atgaaggaag gtgcaggcat aactatacgt 120 aaggtaaatc ctgtggaact gataatagcg cctcgcctgg cacctgagct ttattaa 177 54 58 PRT NICOTIANATABACUM 54 Met Thr Tyr Ala Leu Gln Val Glu His Leu Thr Met Ala His Leu Ile 1 5 10 15 Gln Gly Phe Asn Tyr Arg Thr Pro Asn Asp Glu Pro Leu Asp Met Lys 20 25 30 Glu Gly Ala Gly Ile Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile 35 40 45 Ile Ala Pro Arg Leu Ala Pro Glu Leu Tyr 50 55 55 174 DNA NICOTIANATABACUM 55 atgacttatg cattgcaagt ggaacactta acaatggcac atttgatcca aggtttcaat 60 tacagaactc caaatgacga gcccttggat atgaaggaag gtgcaggcat aactatacgt 120 aaggtaaatc ctgtggaact gataatagcg cccctggcac ctgagcttta ttaa 174 56 57 PRT NICOTIANATABACUM 56 Met Thr Tyr Ala Leu Gln Val Glu His Leu Thr Met Ala His Leu Ile 1 5 10 15 Gln Gly Phe Asn Tyr Arg Thr Pro Asn Asp Glu Pro Leu Asp Met Lys 20 25 30 Glu Gly Ala Gly Ile Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile 35 40 45 Ile Ala Pro Leu Ala Pro Glu Leu Tyr 50 55 57 177 DNA NICOTIANATABACUM 57 atgacttatg cattgcaagt ggaacactta acaatggcac atttgatcca aggtttcaat 60 tacagaactc caaatgacga gcccttggat atgaaggaag gtgcaggcat aactatacgt 120 aaggtaaatc ctgcggaact gataatagcg cctcgcctgg cacctgagct ttattaa 177 58 58 PRT NICOTIANATABACUM 58 Met Thr Tyr Ala Leu Gln Val Glu His Leu Thr Met Ala His Leu Ile 1 5 10 15 Gln Gly Phe Asn Tyr Arg Thr Pro Asn Asp Glu Pro Leu Asp Met Lys 20 25 30 Glu Gly Ala Gly Ile Thr Ile Arg Lys Val Asn Pro Ala Glu Leu Ile 35 40 45 Ile Ala Pro Arg Leu Ala Pro Glu Leu Tyr 50 55 59 177 DNA NICOTIANATABACUM 59 atgacttatg cattacaagt ggaacaccta acaatagcac atttgatcca gggtttcaat 60 tacaaaactc caaatgacga gcccttggat atgaaggaag gtgcaggatt aaccatacgt 120 aaagtaaatc ctgtagaagt gacaactacg gctcgcctgg cacctgagct ttattaa 177 60 58 PRT NICOTIANATABACUM 60 Met Thr Tyr Ala Leu Gln Val Glu His Leu Thr Ile Ala His Leu Ile 1 5 10 15 Gln Gly Phe Asn Tyr Lys Thr Pro Asn Asp Glu Pro Leu Asp Met Lys 20 25 30 Glu Gly Ala Gly Leu Thr Ile Arg Lys Val Asn Pro Val Glu Val Thr 35 40 45 Thr Thr Ala Arg Leu Ala Pro Glu Leu Tyr 50 55 61 177 DNA NICOTIANATABACUM 61 atgacttatg cattacaagt ggaacaccta acaatagcac atttgatcca gggtttcaat 60 tacaaaactc caaatgacga gcccttggat atgaaggaag gtgcaggatt aactatacgt 120 aaagtaaatc ctgtagaagt gacaattacg gctcgcctgg cacctgagct ttattaa 177 62 58 PRT NICOTIANATABACUM 62 Met Thr Tyr Ala Leu Gln Val Glu His Leu Thr Ile Ala His Leu Ile 1 5 10 15 Gln Gly Phe Asn Tyr Lys Thr Pro Asn Asp Glu Pro Leu Asp Met Lys 20 25 30 Glu Gly Ala Gly Leu Thr Ile Arg Lys Val Asn Pro Val Glu Val Thr 35 40 45 Ile Thr Ala Arg Leu Ala Pro Glu Leu Tyr 50 55 63 177 DNA NICOTIANATABACUM 63 atgacttatg cattacaagt ggaacaccta acaatagcac atttgatcca gggtttcaat 60 tacaaaactc caaatgacga gcccctggat atgaaggaag gtgcaggatt aactatacgt 120 aaagtaaatc ctgtagaagt gacaattacg gctcgcctgg cacctgagct ttattaa 177 64 58 PRT NICOTIANATABACUM 64 Met Thr Tyr Ala Leu Gln Val Glu His Leu Thr Ile Ala His Leu Ile 1 5 10 15 Gln Gly Phe Asn Tyr Lys Thr Pro Asn Asp Glu Pro Leu Asp Met Lys 20 25 30 Glu Gly Ala Gly Leu Thr Ile Arg Lys Val Asn Pro Val Glu Val Thr 35 40 45 Ile Thr Ala Arg Leu Ala Pro Glu Leu Tyr 50 55 65 198 DNA NICOTIANATABACUM 65 tatagccttg gacttaaggt tatccgagta acattagcca acatgttgca tggattcaac 60 tggaaattac ctgaaggtat gaagccagaa gatataagtg tggaagaaca ttatgggctc 120 actacacatc ctaagtttcc tgttcctgtg atcttggaat ctagactttc ttcagatctc 180 tattccccca tcacttaa 198 66 65 PRT NICOTIANATABACUM 66 Tyr Ser Leu Gly Leu Lys Val Ile Arg Val Thr Leu Ala Asn Met Leu 1 5 10 15 His Gly Phe Asn Trp Lys Leu Pro Glu Gly Met Lys Pro Glu Asp Ile 20 25 30 Ser Val Glu Glu His Tyr Gly Leu Thr Thr His Pro Lys Phe Pro Val 35 40 45 Pro Val Ile Leu Glu Ser Arg Leu Ser Ser Asp Leu Tyr Ser Pro Ile 50 55 60 Thr 65 67 190 DNA NICOTIANATABACUM 67 atacagtctt gggattcgta taattagggc aactttagct aacttgttgc atggattcaa 60 ctggagattg cctaatggta tgagtccaga agacattagc atggaagaga tttatgggct 120 aattacacac cccaaagtcg cacttgacgt gatgatggag cctcgacttc ccaaccatct 180 ttacaaatag 190 68 62 PRT NICOTIANATABACUM 68 Tyr Ser Leu Gly Ile Arg Ile Ile Arg Ala Thr Leu Ala Asn Leu Leu 1 5 10 15 His Gly Phe Asn Trp Arg Leu Pro Asn Gly Met Ser Pro Glu Asp Ile 20 25 30 Ser Met Glu Glu Ile Tyr Gly Leu Ile Thr His Pro Lys Val Ala Leu 35 40 45 Asp Val Met Met Glu Pro Arg Leu Pro Asn His Leu Tyr Lys 50 55 60 69 174 DNA NICOTIANATABACUM 69 attaattttt caataccact tgttgagctt gcacttgcta atctattgtt tcattataat 60 tggtcacttc ctgaagggat gctagctaag gatgttgata tggaagaagc tttggggatt 120 accatgcaca agaaatctcc cctttgctta gtagcttctc attatacttg ttga 174 70 57 PRT NICOTIANATABACUM 70 Ile Asn Phe Ser Ile Pro Leu Val Glu Leu Ala Leu Ala Asn Leu Leu 1 5 10 15 Phe His Tyr Asn Trp Ser Leu Pro Glu Gly Met Leu Ala Lys Asp Val 20 25 30 Asp Met Glu Glu Ala Leu Gly Ile Thr Met His Lys Lys Ser Pro Leu 35 40 45 Cys Leu Val Ala Ser His Tyr Thr Cys 50 55 71 190 DNA NICOTIANATABACUM 71 atgcaacttg ggctttatgc attggaaatg gctgtggccc atcttcttca ttgttttact 60 tgggaattgc cagatggtat gaaaccaagt gagcttaaaa tggatgatat ttttggactc 120 actgctccaa aagctaatcg actcgtggct gtgcctactc cacgtttgtt gtgtcccctt 180 tattaattga 190 72 61 PRT NICOTIANATABACUM 72 Met Gln Leu Gly Leu Tyr Ala Leu Glu Met Ala Val Ala His Leu Leu 1 5 10 15 His Cys Phe Thr Trp Glu Leu Pro Asp Gly Met Lys Pro Ser Glu Leu 20 25 30 Lys Met Asp Asp Ile Phe Gly Leu Thr Ala Pro Lys Ala Asn Arg Leu 35 40 45 Val Ala Val Pro Thr Pro Arg Leu Leu Cys Pro Leu Tyr 50 55 60 73 186 DNA NICOTIANATABACUM 73 atgcaacttg ggctttatgc attagaaatg gcagtggccc atcttcttct ttgctttact 60 tgggaattgc cagatggtat gaaaccaagt gagcttaaaa tggatgatat ttttggactc 120 actgctccaa gagctaatcg actcgtggct gtgcctagtc cacgtttgtt gtgcccactt 180 tattaa 186 74 61 PRT NICOTIANATABACUM 74 Met Gln Leu Gly Leu Tyr Ala Leu Glu Met Ala Val Ala His Leu Leu 1 5 10 15 Leu Cys Phe Thr Trp Glu Leu Pro Asp Gly Met Lys Pro Ser Glu Leu 20 25 30 Lys Met Asp Asp Ile Phe Gly Leu Thr Ala Pro Arg Ala Asn Arg Leu 35 40 45 Val Ala Val Pro Ser Pro Arg Leu Leu Cys Pro Leu Tyr 50 55 60 75 186 DNA NICOTIANATABACUM 75 atgcaacttg ggctttatgc attggaaatg gctgtggccc atcttcttca ttgttttact 60 tgggaattgc cagatggtat gaaaccaagt gagcttaaaa tggatgatat ttttggactc 120 actgctccaa gagctaatcg actcgtggct gtgcctactc cacgtttgtt gtgtcccctt 180 tattaa 186 76 61 PRT NICOTIANATABACUM 76 Met Gln Leu Gly Leu Tyr Ala Leu Glu Met Ala Val Ala His Leu Leu 1 5 10 15 His Cys Phe Thr Trp Glu Leu Pro Asp Gly Met Lys Pro Ser Glu Leu 20 25 30 Lys Met Asp Asp Ile Phe Gly Leu Thr Ala Pro Arg Ala Asn Arg Leu 35 40 45 Val Ala Val Pro Thr Pro Arg Leu Leu Cys Pro Leu Tyr 50 55 60 77 93 DNA NICOTIANATABACUM 77 atgctttgga gtgcgagtat agtgcgcgtc agctacctaa cttgtattta tagattccaa 60 gtatatgctg ggtctgtgtt cagagtagca tga 93 78 30 PRT NICOTIANATABACUM 78 Met Leu Trp Ser Ala Ser Ile Val Arg Val Ser Tyr Leu Thr Cys Ile 1 5 10 15 Tyr Arg Phe Gln Val Tyr Ala Gly Ser Val Phe Arg Val Ala 20 25 30 79 93 DNA NICOTIANATABACUM 79 atgctttgga gtgcgagtat agtgcgcgtc agctacctaa cttgtattta tagattccaa 60 gtatatgctg ggtctgtgtt cagagtagca tga 93 80 30 PRT NICOTIANATABACUM 80 Met Leu Trp Ser Ala Ser Ile Val Arg Val Ser Tyr Leu Thr Cys Ile 1 5 10 15 Tyr Arg Phe Gln Val Tyr Ala Gly Ser Val Phe Arg Val Ala 20 25 30 81 93 DNA NICOTIANATABACUM 81 atgctttgga gtgcgagtat agtgcgcgtc agctacctaa cctgtattta tagattccaa 60 gtatatgctg ggtctgtgtt cagagtagca tga 93 82 30 PRT NICOTIANATABACUM 82 Met Leu Trp Ser Ala Ser Ile Val Arg Val Ser Tyr Leu Thr Cys Ile 1 5 10 15 Tyr Arg Phe Gln Val Tyr Ala Gly Ser Val Phe Arg Val Ala 20 25 30 83 177 DNA NICOTIANATABACUM 83 attaattttt caataccact tgttgagctt gcacttgcta atctattgtt tcattataat 60 tggtcacttc ctgaggggat gctacctaag gatgttgata tggaagaagc tttggggatt 120 accatgcaca agaaatctcc cctttgctta gtagcttctc attataactt gttgtga 177 84 58 PRT NICOTIANATABACUM 84 Ile Asn Phe Ser Ile Pro Leu Val Glu Leu Ala Leu Ala Asn Leu Leu 1 5 10 15 Phe His Tyr Asn Trp Ser Leu Pro Glu Gly Met Leu Pro Lys Asp Val 20 25 30 Asp Met Glu Glu Ala Leu Gly Ile Thr Met His Lys Lys Ser Pro Leu 35 40 45 Cys Leu Val Ala Ser His Tyr Asn Leu Leu 50 55 85 183 DNA NICOTIANATABACUM 85 atatcatttg gtttggctaa tgtttatttg ccactagctc aattgttata tcattttgat 60 tggaaactcc ctactggaat caattcaagt gacttggaca tgactgagtc gtcaggagta 120 acttgtgcta gaaagagtga tttatacttg actgctactc catatcaact ttctcaagag 180 tga 183 86 61 PRT NICOTIANATABACUM 86 Gly Ile Ser Phe Gly Leu Ala Asn Val Tyr Leu Pro Leu Ala Gln Leu 1 5 10 15 Leu Tyr His Phe Asp Trp Lys Leu Pro Thr Gly Ile Asn Ser Ser Asp 20 25 30 Leu Asp Met Thr Glu Ser Ser Gly Val Thr Cys Ala Arg Lys Ser Asp 35 40 45 Leu Tyr Leu Thr Ala Thr Pro Tyr Gln Leu Ser Gln Glu 50 55 60 87 93 DNA NICOTIANATABACUM 87 atgctttgga gtgcgagtat agtgcgcgtc agctacctaa cttgtattta tagattccaa 60 gtatatgctg ggtctgtgtc cagagtagca tga 93 88 30 PRT NICOTIANATABACUM 88 Met Leu Trp Ser Ala Ser Ile Val Arg Val Ser Tyr Leu Thr Cys Ile 1 5 10 15 Tyr Arg Phe Gln Val Tyr Ala Gly Ser Val Ser Arg Val Ala 20 25 30 89 153 DNA NICOTIANATABACUM 89 ctgaattttg caatgttaga ggcaaaaatg gcacttgcat tgattctaca acactatgct 60 tttgagctct ctccatctta tgcacatgct cctcatacaa ttatcactct gcaacctcaa 120 catggtgctc ctttgatttt gcgcaagctg tag 153 90 50 PRT NICOTIANATABACUM 90 Leu Asn Phe Ala Met Leu Glu Ala Lys Met Ala Leu Ala Leu Ile Leu 1 5 10 15 Gln His Tyr Ala Phe Glu Leu Ser Pro Ser Tyr Ala His Ala Pro His 20 25 30 Thr Ile Ile Thr Leu Gln Pro Gln His Gly Ala Pro Leu Ile Leu Arg 35 40 45 Lys Leu 50 91 153 DNA NICOTIANATABACUM 91 ctgaattttg caatgttaga ggcaaaaatg gcacttgcat tgattctaca acactatgct 60 tttgagctct ctccatctta tgcacacgct cctcatacaa ttatcactct gcaacctcaa 120 catggtgctc ctttgatttt gcgcaagctg tag 153 92 50 PRT NICOTIANATABACUM 92 Leu Asn Phe Ala Met Leu Glu Ala Lys Met Ala Leu Ala Leu Ile Leu 1 5 10 15 Gln His Tyr Ala Phe Glu Leu Ser Pro Ser Tyr Ala His Ala Pro His 20 25 30 Thr Ile Ile Thr Leu Gln Pro Gln His Gly Ala Pro Leu Ile Leu Arg 35 40 45 Lys Leu 50 93 226 DNA NICOTIANATABACUM 93 aataattttg caatgttgga aactaagatt gccttagcaa tgatcctaca gcgttttgct 60 ttcgagcttt ctccatctta cgctcatgca cctacttatg tcgtcactct tcgacctcag 120 tgtggtgctc acttaatctt gcaaaaatta taggtcctta atctggattt cccattattg 180 agtagtgcct aataaatctt ctctatcact atttttccat ctttca 226 94 50 PRT NICOTIANATABACUM 94 Asn Asn Phe Ala Met Leu Glu Thr Lys Ile Ala Leu Ala Met Ile Leu 1 5 10 15 Gln Arg Phe Ala Phe Glu Leu Ser Pro Ser Tyr Ala His Ala Pro Thr 20 25 30 Tyr Val Val Thr Leu Arg Pro Gln Cys Gly Ala His Leu Ile Leu Gln 35 40 45 Lys Leu 50 95 268 DNA NICOTIANATABACUM 95 agcgaagggg tggcaaaggc aacaaagggg aaaatgacat attttccatt tggtgcagga 60 ccgcgaaaat gcattgggca aaacttcgcg attttggaag caaaaatggc tatagctatg 120 attctacaac gcttctcctt cgagctctcc ccatcttata cacactctcc atacactgtg 180 gtcactttga aacccaaata tggtgctccc ctaataatgc acaggctgta gtcctgtgag 240 aatatgctat ccgaggaatt cagttcct 268 96 50 PRT NICOTIANATABACUM 96 Gln Asn Phe Ala Ile Leu Glu Ala Lys Met Ala Ile Ala Met Ile Leu 1 5 10 15 Gln Arg Phe Ser Phe Glu Leu Ser Pro Ser Tyr Thr His Ser Pro Tyr 20 25 30 Thr Val Val Thr Leu Lys Pro Lys Tyr Gly Ala Pro Leu Ile Met His 35 40 45 Arg Leu 50 97 237 DNA NICOTIANATABACUM 97 agcgaagggg tggcaaaggc aacaaagggg aaaatgacat attttccatt tggtgcagga 60 ccgcgaaaat gcattgggca aaacttcgcg attttggaag caaaaatggc tatagctatg 120 attctacaac gcttctcctt cgagctctct ccatcttata cacactctcc atacactgtg 180 gtcactttga aacccaaata tggtgctccc ctaataatgc acaggctgta gtcctgt 237 98 50 PRT NICOTIANATABACUM 98 Gln Asn Phe Ala Ile Leu Glu Ala Lys Met Ala Ile Ala Met Ile Leu 1 5 10 15 Gln Arg Phe Ser Phe Glu Leu Ser Pro Ser Tyr Thr His Ser Pro Tyr 20 25 30 Thr Val Val Thr Leu Lys Pro Lys Tyr Gly Ala Pro Leu Ile Met His 35 40 45 Arg Leu 50 99 152 DNA NICOTIANATABACUM 99 caaaattttg ccatgttaga agcaaagatg gctctgtcta tgatcctgca acgcttctct 60 tttgaactgt ctccgtctta tgcacatgcc cctcagtcca tattaaccgt cagccacaat 120 atggtgctcc acttattttc cacaagctat aa 152 100 50 PRT NICOTIANATABACUM 100 Gln Asn Phe Ala Met Leu Glu Ala Lys Met Ala Leu Ser Met Ile Leu 1 5 10 15 Gln Arg Phe Ser Phe Glu Leu Ser Pro Ser Tyr Ala His Ala Pro Gln 20 25 30 Ser Ile Leu Thr Val Gln Pro Gln Tyr Gly Ala Pro Leu Ile Phe His 35 40 45 Lys Leu 50 101 153 DNA NICOTIANATABACUM 101 ataaactttg caatgacaga agcgaagatg gctatggcta tgattctgca acgcttctcc 60 tttgagctat ctccatctta cacacatgct ccacagtctg taataactat gcaaccccaa 120 tatggtgctc ctcttatatt gcacaaattg taa 153 102 50 PRT NICOTIANATABACUM 102 Ile Asn Phe Ala Met Thr Glu Ala Lys Met Ala Met Ala Met Ile Leu 1 5 10 15 Gln Arg Phe Ser Phe Glu Leu Ser Pro Ser Tyr Thr His Ala Pro Gln 20 25 30 Ser Val Ile Thr Met Gln Pro Gln Tyr Gly Ala Pro Leu Ile Leu His 35 40 45 Lys Leu 50 103 153 DNA NICOTIANATABACUM 103 ataaactttg caatggcaga agcgaagatg gctatggcta tgattctgca acgcttctcc 60 tttgagctat ctccatctta cacacatgct ccacagtctg taataactat gcaaccccaa 120 tatggtgctc ctcttatatt gcacaaattg taa 153 104 50 PRT NICOTIANATABACUM 104 Ile Asn Phe Ala Met Ala Glu Ala Lys Met Ala Met Ala Met Ile Leu 1 5 10 15 Gln Arg Phe Ser Phe Glu Leu Ser Pro Ser Tyr Thr His Ala Pro Gln 20 25 30 Ser Val Ile Thr Met Gln Pro Gln Tyr Gly Ala Pro Leu Ile Leu His 35 40 45 Lys Leu 50 105 153 DNA NICOTIANATABACUM 105 caaaactttg caatgatgga agcaaaaatg gcagtagcta tgatactaca aaaattttcc 60 tttgaactat ccccttctta tacacatgct ccatttgcaa ttgtgactat tcatcctcag 120 tatggtgctc ctctgcttat gcgcagactt taa 153 106 50 PRT NICOTIANATABACUM 106 Gln Asn Phe Ala Met Met Glu Ala Lys Met Ala Val Ala Met Ile Leu 1 5 10 15 Gln Lys Phe Ser Phe Glu Leu Ser Pro Ser Tyr Thr His Ala Pro Phe 20 25 30 Ala Ile Val Thr Ile His Pro Gln Tyr Gly Ala Pro Leu Leu Met Arg 35 40 45 Arg Leu 50 107 153 DNA NICOTIANATABACUM 107 caaaactttg caatgatgga agcaaaaatg gcagtagcta tgatactaca taaattttcc 60 tttgaactat ccccttctta tacacatgct ccatttgcaa ttgtgactat tcatcctcag 120 tatggtgctc ctctgcttat gcgcagactt taa 153 108 49 PRT NICOTIANATABACUM 108 Gln Asn Phe Ala Met Met Glu Ala Lys Met Ala Val Ala Met Ile Leu 1 5 10 15 His Lys Phe Ser Phe Glu Leu Pro Ser Tyr Thr His Ala Pro Phe Ala 20 25 30 Ile Val Thr Ile His Pro Gln Tyr Gly Ala Pro Leu Leu Met Arg Arg 35 40 45 Leu 109 150 DNA NICOTIANATABACUM 109 caaaattttg ctatgttaga ggctaaaatg gcaatggcta tgattctgaa aacctatgca 60 tttgaactct ctccatctta tgctcatgct cctcatccac tactacttca acctcaatat 120 ggtgctcaat taattttgta caagttgtag 150 110 49 PRT NICOTIANATABACUM 110 Gln Asn Phe Ala Met Leu Glu Ala Lys Met Ala Met Ala Met Ile Leu 1 5 10 15 Lys Thr Tyr Ala Phe Glu Leu Ser Pro Ser Tyr Ala His Ala Pro His 20 25 30 Pro Leu Leu Leu Gln Pro Gln Tyr Gly Ala Gln Leu Ile Leu Tyr Lys 35 40 45 Leu 111 192 DNA NICOTIANATABACUM 111 tatagcatgg ggctcaaggc gattcaagct agcttagcta atcttctaca tggatttaac 60 tggtcattgc ctgataatat gactcctgag gacctcaaca tggatgagat ttttgggctc 120 tctacaccta aaaaatttcc acttgctact gtgattgagc caagactttc accaaaactt 180 tactctgttt ga 192 112 63 PRT NICOTIANATABACUM 112 Tyr Ser Met Gly Leu Lys Ala Ile Gln Ala Ser Leu Ala Asn Leu Leu 1 5 10 15 His Gly Phe Asn Trp Ser Leu Pro Asp Asn Met Thr Pro Glu Asp Leu 20 25 30 Asn Met Asp Glu Ile Phe Gly Leu Ser Thr Pro Lys Lys Phe Pro Leu 35 40 45 Ala Thr Val Ile Glu Pro Arg Leu Ser Pro Lys Leu Tyr Ser Val 50 55 60 113 192 DNA NICOTIANATABACUM 113 tatagcttgg ggctcaagga gattcaagct agcttagcta atcttctaca tggatttaac 60 tggtcattgc ctgataatat gactcctgag gacctcaaca tggatgagat ttttgggctc 120 tctacaccta aaaaatttcc acttgctact gtgattgagc caagactttc accaaaactt 180 tactctgttt ga 192 114 63 PRT NICOTIANATABACUM 114 Tyr Ser Leu Gly Leu Lys Glu Ile Gln Ala Ser Leu Ala Asn Leu Leu 1 5 10 15 His Gly Phe Asn Trp Ser Leu Pro Asp Asn Met Thr Pro Glu Asp Leu 20 25 30 Asn Met Asp Glu Ile Phe Gly Leu Ser Thr Pro Lys Lys Phe Pro Leu 35 40 45 Ala Thr Val Ile Glu Pro Arg Leu Ser Pro Lys Leu Tyr Ser Val 50 55 60 115 192 DNA NICOTIANATABACUM 115 catagcttgg ggctcaaggt gattcaagct agcttagcta atcttctaca tggatttaac 60 tggtcattgc ctgataatat gactcctgag gacctcaaca tggatgagat ttttgggctc 120 tctacaccta aaaaatttcc acttgctact gtgattgagc caagactttc accaaaactt 180 tactctgttt ga 192 116 63 PRT NICOTIANATABACUM 116 His Ser Leu Gly Leu Lys Val Ile Gln Ala Ser Leu Ala Asn Leu Leu 1 5 10 15 His Gly Phe Asn Trp Ser Leu Pro Asp Asn Met Thr Pro Glu Asp Leu 20 25 30 Asn Met Asp Glu Ile Phe Gly Leu Ser Thr Pro Lys Lys Phe Pro Leu 35 40 45 Ala Thr Val Ile Glu Pro Arg Leu Ser Pro Lys Leu Tyr Ser Val 50 55 60 117 96 DNA NICOTIANATABACUM 117 ctgtgctttc catgtttaat ctctagttat atactggctt tgaatgtgaa tctgtatcat 60 aatttcttgc aaatttctcc ttccatttct tattaa 96 118 31 PRT NICOTIANATABACUM 118 Leu Cys Phe Pro Cys Leu Ile Ser Ser Tyr Ile Leu Ala Leu Asn Val 1 5 10 15 Asn Leu Tyr His Asn Phe Leu Gln Ile Ser Pro Ser Ile Ser Tyr 20 25 30 119 192 DNA NICOTIANATABACUM 119 tctggacttg ctcaatgtgt ggttggttta gctttagcaa ctctagtgca gtgttttgag 60 tggaaaaggg taagcgaaga ggtggttgat ttgacggaag gaaaaggtct cactatgcca 120 aaacccgagc cactcatggc taggtgcgaa gctcgtgaca tttttcacaa agttctttca 180 gaaatatctt aa 192 120 63 PRT NICOTIANATABACUM 120 Ser Gly Leu Ala Gln Cys Val Val Gly Leu Ala Leu Ala Thr Leu Val 1 5 10 15 Gln Cys Phe Glu Trp Lys Arg Val Ser Glu Glu Val Val Asp Leu Thr 20 25 30 Glu Gly Lys Gly Leu Thr Met Pro Lys Pro Glu Pro Leu Met Ala Arg 35 40 45 Cys Glu Ala Arg Asp Ile Phe His Lys Val Leu Ser Glu Ile Ser 50 55 60 121 174 DNA NICOTIANATABACUM 121 ttgggcttgg caacggtgca tgtgaatttg atgttggccc gaatgattca agaatttgaa 60 tggtccgctt acccggaaaa taggaaagtg gattttactg agaaattgga atttactgtg 120 gtgatgaaaa atcctttaag agctaaggtc aagccaagaa tgcaagtggt gtaa 174 122 57 PRT NICOTIANATABACUM 122 Leu Gly Leu Ala Thr Val His Val Asn Leu Met Leu Ala Arg Met Ile 1 5 10 15 Gln Glu Phe Glu Trp Ser Ala Tyr Pro Glu Asn Arg Lys Val Asp Phe 20 25 30 Thr Glu Lys Leu Glu Phe Thr Val Val Met Lys Asn Pro Leu Arg Ala 35 40 45 Lys Val Lys Pro Arg Met Gln Val Val 50 55 123 171 DNA NICOTIANATABACUM 123 tatgctttgg ctatgcttca tttagagtac tttgtggcta atttggtttg gcattttcga 60 tgggaggctg tggagggaga tgatgttgat ctttcagaaa agctagaatt caccgttgtg 120 atgaagaatc cacttcgagc tcgtatctgc cccagagtta actctatttg a 171 124 56 PRT NICOTIANATABACUM 124 Tyr Ala Leu Ala Met Leu His Leu Glu Tyr Phe Val Ala Asn Leu Val 1 5 10 15 Trp His Phe Arg Trp Glu Ala Val Glu Gly Asp Asp Val Asp Leu Ser 20 25 30 Glu Lys Leu Glu Phe Thr Val Val Met Lys Asn Pro Leu Arg Ala Arg 35 40 45 Ile Cys Pro Arg Val Asn Ser Ile 50 55 125 186 DNA NICOTIANATABACUM 125 ggtcagcaag ttggacttct tagaacaacc attttcatcg cctcattact gtctgaatat 60 aagctgaaac ctcgctcaca ccagaaacaa gttgaactca ccgatttaaa tccagcaagt 120 tggcttcatt cgataaaagg cgaactgtta gtcgatgcga ttcctcgaaa gaaggcggca 180 ttttaa 186 126 61 PRT NICOTIANATABACUM 126 Gly Gln Gln Val Gly Leu Leu Arg Thr Thr Ile Phe Ile Ala Ser Leu 1 5 10 15 Leu Ser Glu Tyr Lys Leu Lys Pro Arg Ser His Gln Lys Gln Val Glu 20 25 30 Leu Thr Asp Leu Asn Pro Ala Ser Trp Leu His Ser Ile Lys Gly Glu 35 40 45 Leu Leu Val Asp Ala Ile Pro Arg Lys Lys Ala Ala Phe 50 55 60 127 171 DNA NICOTIANATABACUM 127 atcacatttg ctaagtttgt gaatgagcta gcattggcaa gattaatgtt ccattttgat 60 ttctcgctac caaaaggagt taagcatgag gatttggacg tggaggaagc tgctggaatt 120 actgttagaa ggaagttccc ccttttagcc gtcgccactc catgctcgtg a 171 128 56 PRT NICOTIANATABACUM 128 Ile Thr Phe Ala Lys Phe Val Asn Glu Leu Ala Leu Ala Arg Leu Met 1 5 10 15 Phe His Phe Asp Phe Ser Leu Pro Lys Gly Val Lys His Glu Asp Leu 20 25 30 Asp Val Glu Glu Ala Ala Gly Ile Thr Val Arg Arg Lys Phe Pro Leu 35 40 45 Leu Ala Val Ala Thr Pro Cys Ser 50 55 129 180 DNA NICOTIANATABACUM 129 cagaggtatg ctataaacca tttgatgctc tttattgcgt tgttcacggc tctgattgat 60 ttcaagaggc acaaaacgga cggctgtgat gatatcgcgt atattccaac cattgctcca 120 aaggatgatt gtaaagtgtt cctttcacag aggtgcactc gattcccatc tttttcatga 180 130 59 PRT NICOTIANATABACUM 130 Gln Arg Tyr Ala Ile Asn His Leu Met Leu Phe Ile Ala Leu Phe Thr 1 5 10 15 Ala Leu Ile Asp Phe Lys Arg His Lys Thr Asp Gly Cys Asp Asp Ile 20 25 30 Ala Tyr Ile Pro Thr Ile Ala Pro Lys Asp Asp Cys Lys Val Phe Leu 35 40 45 Ser Gln Arg Cys Thr Arg Phe Pro Ser Phe Ser 50 55 131 183 DNA NICOTIANATABACUM 131 atgtcatttg gtttagctaa tctttactta ccattggctc aattactcta tcactttgac 60 tggaaactcc caaccggaat caagccaaga gacttggact tgaccgaatt atcgggaata 120 actattgcta gaaagggtga cctttactta aatgctactc cttatcaacc ttctcgagag 180 taa 183 132 60 PRT NICOTIANATABACUM 132 Met Ser Phe Gly Leu Ala Asn Leu Tyr Leu Pro Leu Ala Gln Leu Leu 1 5 10 15 Tyr His Phe Asp Trp Lys Leu Pro Thr Gly Ile Lys Pro Arg Asp Leu 20 25 30 Asp Leu Thr Glu Leu Ser Gly Ile Thr Ile Ala Arg Lys Gly Asp Leu 35 40 45 Tyr Leu Asn Ala Thr Pro Tyr Gln Pro Ser Arg Glu 50 55 60 133 174 DNA NICOTIANATABACUM 133 ttgggcttgg caacggtgca tgtgaatttg atgttggccc gaacgattca agaatttgaa 60 tggtccgctt acccggaaaa taggaaagtg gattttactg agaaattgga atttactgtg 120 gtgatgaaaa accctttaag agctaaggtc aagccaagaa tgcaagtggt gtaa 174 134 57 PRT NICOTIANATABACUM 134 Leu Gly Leu Ala Thr Val His Val Asn Leu Met Leu Ala Arg Thr Ile 1 5 10 15 Gln Glu Phe Glu Trp Ser Ala Tyr Pro Glu Asn Arg Lys Val Asp Phe 20 25 30 Thr Glu Lys Leu Glu Phe Thr Val Val Met Lys Asn Pro Leu Arg Ala 35 40 45 Lys Val Lys Pro Arg Met Gln Val Val 50 55 135 123 DNA NICOTIANATABACUM 135 ttgggcttgg caacggtgca tgtgaatttg atgttggccc gaatgattca agaatttgaa 60 tggtccgctt acccggaaaa taggaaagtg gatttactga gaaattggaa tttactgtgg 120 tga 123 136 40 PRT NICOTIANATABACUM 136 Leu Gly Leu Ala Thr Val His Val Asn Leu Met Leu Ala Arg Met Ile 1 5 10 15 Gln Glu Phe Glu Trp Ser Ala Tyr Pro Glu Asn Arg Lys Val Asp Leu 20 25 30 Leu Arg Asn Trp Asn Leu Leu Trp 35 40 137 171 DNA NICOTIANATABACUM 137 atcacatttg ctaagtttgt gaatgagcta gcattggcaa gattaatgtt ccattttgat 60 ttctcgctac caaaaggagt taagcatgag gatttggacg tggaggaagc tgctggaatt 120 actgttagga ggaagttccc ccttttagcc gtcgccactc catgctcgtg a 171 138 56 PRT NICOTIANATABACUM 138 Ile Thr Phe Ala Lys Phe Val Asn Glu Leu Ala Leu Ala Arg Leu Met 1 5 10 15 Phe His Phe Asp Phe Ser Leu Pro Lys Gly Val Lys His Glu Asp Leu 20 25 30 Asp Val Glu Glu Ala Ala Gly Ile Thr Val Arg Arg Lys Phe Pro Leu 35 40 45 Leu Ala Val Ala Thr Pro Cys Ser 50 55 139 171 DNA NICOTIANATABACUM 139 atcacatttg ctaagtttgt gaatgagcta gcattggcaa gattaatgtt ccattttgat 60 ttctcgctac caaaaggagt taagcatgcg gatttggacg tggaggaagc tgctggaatt 120 actgttagaa ggaagttccc ccttttagcc gtcgccactc catgctcgtg a 171 140 56 PRT NICOTIANATABACUM 140 Ile Thr Phe Ala Lys Phe Val Asn Glu Leu Ala Leu Ala Arg Leu Met 1 5 10 15 Phe His Phe Asp Phe Ser Leu Pro Lys Gly Val Lys His Ala Asp Leu 20 25 30 Asp Val Glu Glu Ala Ala Gly Ile Thr Val Arg Arg Lys Phe Pro Leu 35 40 45 Leu Ala Val Ala Thr Pro Cys Ser 50 55 141 153 DNA NICOTIANATABACUM 141 caaaattttg ccatgttaga agcaaagact actttggcta tgatcctaca acgcttctcc 60 tttgaactgt ctccatctta tgcacatgct cctcagtcca taataacttt gcaaccccag 120 tatggtgctc cacttatttt gcataaaata tag 153 142 50 PRT NICOTIANATABACUM 142 Gln Asn Phe Ala Met Leu Glu Ala Lys Thr Thr Leu Ala Met Ile Leu 1 5 10 15 Gln Arg Phe Ser Phe Glu Leu Ser Pro Ser Tyr Ala His Ala Pro Gln 20 25 30 Ser Ile Ile Thr Leu Gln Pro Gln Tyr Gly Ala Pro Leu Ile Leu His 35 40 45 Lys Ile 50 143 171 DNA NICOTIANATABACUM 143 attatccttg cactgccaat tcttggcatt accttgggac gcttggtgca gaactttgag 60 ttgttgcctc ctccaggaca gtcaaagctt gacacaacag agaaaggcgg gcaattcagt 120 ctgcacattt tgaagcattc caccattgtg atgaaaccaa gatcttttta a 171 144 56 PRT NICOTIANATABACUM 144 Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Leu Gly Arg Leu Val 1 5 10 15 Gln Asn Phe Glu Leu Leu Pro Pro Pro Gly Gln Ser Lys Leu Asp Thr 20 25 30 Thr Glu Lys Gly Gly Gln Phe Ser Leu His Ile Leu Lys His Ser Thr 35 40 45 Ile Val Met Lys Pro Arg Ser Phe 50 55 145 267 DNA NICOTIANATABACUM 145 caaaattttg ccatgttaga agcaaagact actttggcta tgatcctaca acgcttctcc 60 tttgaactgt ctccatctta tgcacatgct cctcagtcca taataacttg caaccccagt 120 atggtgctcc acttattttg cataaaatat agtttattac ttgtaagtag tgtctcgttt 180 tatgttaagc atgagtccaa aatgttaagg cttgtagaac tgcaaaatgg gaatgcattt 240 gcactcgtgc actgtagatt gttgtaa 267 146 88 PRT NICOTIANATABACUM 146 Gln Asn Phe Ala Met Leu Glu Ala Lys Thr Thr Leu Ala Met Ile Leu 1 5 10 15 Gln Arg Phe Ser Phe Glu Leu Ser Pro Ser Tyr Ala His Ala Pro Gln 20 25 30 Ser Ile Ile Thr Cys Asn Pro Ser Met Val Leu His Leu Phe Cys Ile 35 40 45 Lys Tyr Ser Leu Leu Leu Val Ser Ser Val Ser Phe Tyr Val Lys His 50 55 60 Glu Ser Lys Met Leu Arg Leu Val Glu Leu Gln Asn Gly Asn Ala Phe 65 70 75 80 Ala Leu Val His Cys Arg Leu Leu 85 147 186 DNA NICOTIANATABACUM 147 gctgatatgg ggttgcgagc agtttctttg gcattaggtg cacttattca atgctttgac 60 tggcaaattg aggaagcgga aagcttggag gaaagctata attctagaat gactatgcag 120 aacaagcctt tgaaggttgt ctgcactcca cgcgaagatc ttggccagct tctatcccaa 180 ctctaa 186 148 61 PRT NICOTIANATABACUM 148 Ala Asp Met Gly Leu Arg Ala Val Ser Leu Ala Leu Gly Ala Leu Ile 1 5 10 15 Gln Cys Phe Asp Trp Gln Ile Glu Glu Ala Glu Ser Leu Glu Glu Ser 20 25 30 Tyr Asn Ser Arg Met Thr Met Gln Asn Lys Pro Leu Lys Val Val Cys 35 40 45 Thr Pro Arg Glu Asp Leu Gly Gln Leu Leu Ser Gln Leu 50 55 60 149 1576 DNA NICOTIANATABACUM 149 cttccttcct aagtcctaac taaaaatgga gattcagttt tctaacttag ttgcattctt 60 gctctttctc tccagcatct ttcttctatt caaaaaatgg aaaaccagaa aactaaattt 120 gcctcctggt ccatggaaat taccttttat tggaagttta caccatttgg ctgtggcagg 180 tccacttcct caccatggcc taaaaaattt agccaaacgc tatggtcctc ttatgcattt 240 acaacttgga caaattccta cactcatcat atcatcacct caaatggcaa aagaagtact 300 aaaaactcac gacctcgctt ttgccactag accaaagctt gtcgcggccg acatcattca 360 ctacgacagc acggacatag cattttctcc gtacggtgaa tactggagac aaattcgtaa 420 aatttgcata ttggaactct tgagtgccaa gatggtcaaa ttttttagct cgattcgcca 480 agatgagctc tcgaagatgc tctcatctat acgaacgaca cccaatctta cagtcaatct 540 tactgacaaa attttttggt ttacgagttc ggtaacttgt agatcagctt tagggaagat 600 atgtggtgac caagacaaat tgatcatttt tatgagggaa ataatatcat tggcaggtgg 660 atttagtatt gctgattttt tccctacatg gaaaatgatt catgatattg atggttcgaa 720 atctaaactg gtgaaagcac atcgtaagat tgatgaaatt ttgggaaatg ttgttgatga 780 gcacaaaaag aacagagcag atggcaagaa gggtaatggt gaatttggtg gtgaagattt 840 gattgatgta ttgttaagag ttagagaaag tggagaagtt caaattccta tcacaaatga 900 caatatcaaa tcaatattaa tcgacatgtt ctctgcagga tctgaaacat catcgacgac 960 tataatttgg gcattagctg aaatgatgaa gaaaccaagt gttttagcaa aggcacaagc 1020 tgaagtaagg caagctttga aggagaaaaa aggttttcaa cagattgatc ttgatgagct 1080 aaaatatctc aagttagtaa tcaaagaaac cttaagaatg caccctccaa ttcctctatt 1140 agttcctaga gaatgtatgg aggatacaaa gattgatggt tacaatatac ctttcaaaac 1200 aagagtcata gttaatgcat gggcaatcgg acgagatcca gaaagttggg atgaccccga 1260 aagctttatg ccagagagat ttgagaatag ttctattgac tttcttggaa atcatcatca 1320 gtttatacca tttggtgcag gaagaaggat ttgtccggga atgctatttg gtttagctaa 1380 tgttggacaa cctttagctc agttacttta tcacttcgat tggaaactcc ctaatggaca 1440 aagtcatgag aatttcgaca tgactgagtc acctggaatt tctgctacaa gaaaggatga 1500 tcttgttttg attgccactc cttatgattc ttattaagca gtagcagaaa taaaaagccg 1560 gggcaaacag aaaaaa 1576 150 503 PRT NICOTIANATABACUM 150 Met Glu Ile Gln Phe Ser Asn Leu Val Ala Phe Leu Leu Phe Leu Ser 1 5 10 15 Ser Ile Phe Leu Leu Phe Lys Lys Trp Lys Thr Arg Lys Leu Asn Leu 20 25 30 Pro Pro Gly Pro Trp Lys Leu Pro Phe Ile Gly Ser Leu His His Leu 35 40 45 Ala Val Ala Gly Pro Leu Pro His His Gly Leu Lys Asn Leu Ala Lys 50 55 60 Arg Tyr Gly Pro Leu Met His Leu Gln Leu Gly Gln Ile Pro Thr Leu 65 70 75 80 Ile Ile Ser Ser Pro Gln Met Ala Lys Glu Val Leu Lys Thr His Asp 85 90 95 Leu Ala Phe Ala Thr Arg Pro Lys Leu Val Ala Ala Asp Ile Ile His 100 105 110 Tyr Asp Ser Thr Asp Ile Ala Phe Ser Pro Tyr Gly Glu Tyr Trp Arg 115 120 125 Gln Ile Arg Lys Ile Cys Ile Leu Glu Leu Leu Ser Ala Lys Met Val 130 135 140 Lys Phe Phe Ser Ser Ile Arg Gln Asp Glu Leu Ser Lys Met Leu Ser 145 150 155 160 Ser Ile Arg Thr Thr Pro Asn Leu Thr Val Asn Leu Thr Asp Lys Ile 165 170 175 Phe Trp Phe Thr Ser Ser Val Thr Cys Arg Ser Ala Leu Gly Lys Ile 180 185 190 Cys Gly Asp Gln Asp Lys Leu Ile Ile Phe Met Arg Glu Ile Ile Ser 195 200 205 Leu Ala Gly Gly Phe Ser Ile Ala Asp Phe Phe Pro Thr Trp Lys Met 210 215 220 Ile His Asp Ile Asp Gly Ser Lys Ser Lys Leu Val Lys Ala His Arg 225 230 235 240 Lys Ile Asp Glu Ile Leu Gly Asn Val Val Asp Glu His Lys Lys Asn 245 250 255 Arg Ala Asp Gly Lys Lys Gly Asn Gly Glu Phe Gly Gly Glu Asp Leu 260 265 270 Ile Asp Val Leu Leu Arg Val Arg Glu Ser Gly Glu Val Gln Ile Pro 275 280 285 Ile Thr Asn Asp Asn Ile Lys Ser Ile Leu Ile Asp Met Phe Ser Ala 290 295 300 Gly Ser Glu Thr Ser Ser Thr Thr Ile Ile Trp Ala Leu Ala Glu Met 305 310 315 320 Met Lys Lys Pro Ser Val Leu Ala Lys Ala Gln Ala Glu Val Arg Gln 325 330 335 Ala Leu Lys Glu Lys Lys Gly Phe Gln Gln Ile Asp Leu Asp Glu Leu 340 345 350 Lys Tyr Leu Lys Leu Val Ile Lys Glu Thr Leu Arg Met His Pro Pro 355 360 365 Ile Pro Leu Leu Val Pro Arg Glu Cys Met Glu Asp Thr Lys Ile Asp 370 375 380 Gly Tyr Asn Ile Pro Phe Lys Thr Arg Val Ile Val Asn Ala Trp Ala 385 390 395 400 Ile Gly Arg Asp Pro Glu Ser Trp Asp Asp Pro Glu Ser Phe Met Pro 405 410 415 Glu Arg Phe Glu Asn Ser Ser Ile Asp Phe Leu Gly Asn His His Gln 420 425 430 Phe Ile Pro Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly Met Leu Phe 435 440 445 Gly Leu Ala Asn Val Gly Gln Pro Leu Ala Gln Leu Leu Tyr His Phe 450 455 460 Asp Trp Lys Leu Pro Asn Gly Gln Ser His Glu Asn Phe Asp Met Thr 465 470 475 480 Glu Ser Pro Gly Ile Ser Ala Thr Arg Lys Asp Asp Leu Val Leu Ile 485 490 495 Ala Thr Pro Tyr Asp Ser Tyr 500 151 1581 DNA NICOTIANATABACUM 151 tccttcttcc ttcctaagtc ctaactaaaa atggagattc agttttctaa cttagttgca 60 ttcttgctct ttctctccag catctttctt ctattcaaaa aatggaaaac cagaaaacta 120 aatttgcctc ctggtccatg gaaattacct tttattggaa gtttacacca tttggctgtg 180 gcaggtccac ttcctcacca tggcctaaaa aatttagcca aacgctatgg tcctcttatg 240 catttacaac ttggacaaat tcctacactc atcatatcat cacctcaaat ggcaaaagaa 300 gtactaaaaa ctcacgacct cgcttttgcc actagaccaa agcttgtcgt ggccgacatc 360 attcactacg acagcacgga catagcattt tctccgtacg gtgaatactg gagacaaatt 420 cgtaaaattt gcatattgga actcttgagt gccaagatgg tcaaattttt tagctcgatt 480 cgccaagatg agctctcgaa gatgctctca tctatacgaa cgacacccaa tcttacagtc 540 aatcttactg acaaaatttt ttggtttacg agttcggtaa cttgtagatc agctttaggg 600 aagatatgtg gtgaccaaga caaattgatc atttttatga gggaaataat atcattggca 660 ggtggattta gtattgctga ttttttccct acatggaaaa tgattcatga tattgatggt 720 tcgaaatcta aactggtgaa agcacatcgt aagattgatg aaattttggg aaatgttgtt 780 gatgagcaca aaaagaacag agcagatggc aagaagggta atggtgaatt tggtggtgaa 840 gatttgattg atgtattgtt aagagttaga gaaagtggag aagttcaaat tcctatcaca 900 aatgacaata tcaaatcaat attaatcgac atgttctctg cgggatctga aacatcatcg 960 acgactataa tttgggcatt agctgaaatg atgaagaaac caagtgtttt agcaaaggca 1020 caagctgaag taaggcaagc tttgaaggag aaaaaaggtt ttcaacagat tgatcttgat 1080 gagctaaaat atctcaagtt agtaatcaaa gaaaccttaa gaatgcaccc tccaattcct 1140 ctattagttc ctagagaatg tatggaggat acaaagattg atggttacaa tatacctttc 1200 aaaacaagag tcatagttaa tgcatgggca atcggacgag atccagaaag ttgggatgac 1260 cccgaaagct ttatgccaga gagatttgag aatagttcta ttgactttct tggaaatcat 1320 catcagttta taccatttgg tgcaggaaga aggatttgtc cgggaatgct atttggttta 1380 gctaatgttg gacaaccttt agctcagtta ctttatcact tcgattggaa actccctaat 1440 ggacaaagtc atgagaattt cgacatgact gagtcacctg gaatttctgc tacaagaaag 1500 gatgatcttg ttttgattgc cactccttat gattcttatt aagcagtagc agaaataaaa 1560 agccggggca aacagaaaaa a 1581 152 503 PRT NICOTIANATABACUM 152 Met Glu Ile Gln Phe Ser Asn Leu Val Ala Phe Leu Leu Phe Leu Ser 1 5 10 15 Ser Ile Phe Leu Leu Phe Lys Lys Trp Lys Thr Arg Lys Leu Asn Leu 20 25 30 Pro Pro Gly Pro Trp Lys Leu Pro Phe Ile Gly Ser Leu His His Leu 35 40 45 Ala Val Ala Gly Pro Leu Pro His His Gly Leu Lys Asn Leu Ala Lys 50 55 60 Arg Tyr Gly Pro Leu Met His Leu Gln Leu Gly Gln Ile Pro Thr Leu 65 70 75 80 Ile Ile Ser Ser Pro Gln Met Ala Lys Glu Val Leu Lys Thr His Asp 85 90 95 Leu Ala Phe Ala Thr Arg Pro Lys Leu Val Val Ala Asp Ile Ile His 100 105 110 Tyr Asp Ser Thr Asp Ile Ala Phe Ser Pro Tyr Gly Glu Tyr Trp Arg 115 120 125 Gln Ile Arg Lys Ile Cys Ile Leu Glu Leu Leu Ser Ala Lys Met Val 130 135 140 Lys Phe Phe Ser Ser Ile Arg Gln Asp Glu Leu Ser Lys Met Leu Ser 145 150 155 160 Ser Ile Arg Thr Thr Pro Asn Leu Thr Val Asn Leu Thr Asp Lys Ile 165 170 175 Phe Trp Phe Thr Ser Ser Val Thr Cys Arg Ser Ala Leu Gly Lys Ile 180 185 190 Cys Gly Asp Gln Asp Lys Leu Ile Ile Phe Met Arg Glu Ile Ile Ser 195 200 205 Leu Ala Gly Gly Phe Ser Ile Ala Asp Phe Phe Pro Thr Trp Lys Met 210 215 220 Ile His Asp Ile Asp Gly Ser Lys Ser Lys Leu Val Lys Ala His Arg 225 230 235 240 Lys Ile Asp Glu Ile Leu Gly Asn Val Val Asp Glu His Lys Lys Asn 245 250 255 Arg Ala Asp Gly Lys Lys Gly Asn Gly Glu Phe Gly Gly Glu Asp Leu 260 265 270 Ile Asp Val Leu Leu Arg Val Arg Glu Ser Gly Glu Val Gln Ile Pro 275 280 285 Ile Thr Asn Asp Asn Ile Lys Ser Ile Leu Ile Asp Met Phe Ser Ala 290 295 300 Gly Ser Glu Thr Ser Ser Thr Thr Ile Ile Trp Ala Leu Ala Glu Met 305 310 315 320 Met Lys Lys Pro Ser Val Leu Ala Lys Ala Gln Ala Glu Val Arg Gln 325 330 335 Ala Leu Lys Glu Lys Lys Gly Phe Gln Gln Ile Asp Leu Asp Glu Leu 340 345 350 Lys Tyr Leu Lys Leu Val Ile Lys Glu Thr Leu Arg Met His Pro Pro 355 360 365 Ile Pro Leu Leu Val Pro Arg Glu Cys Met Glu Asp Thr Lys Ile Asp 370 375 380 Gly Tyr Asn Ile Pro Phe Lys Thr Arg Val Ile Val Asn Ala Trp Ala 385 390 395 400 Ile Gly Arg Asp Pro Glu Ser Trp Asp Asp Pro Glu Ser Phe Met Pro 405 410 415 Glu Arg Phe Glu Asn Ser Ser Ile Asp Phe Leu Gly Asn His His Gln 420 425 430 Phe Ile Pro Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly Met Leu Phe 435 440 445 Gly Leu Ala Asn Val Gly Gln Pro Leu Ala Gln Leu Leu Tyr His Phe 450 455 460 Asp Trp Lys Leu Pro Asn Gly Gln Ser His Glu Asn Phe Asp Met Thr 465 470 475 480 Glu Ser Pro Gly Ile Ser Ala Thr Arg Lys Asp Asp Leu Val Leu Ile 485 490 495 Ala Thr Pro Tyr Asp Ser Tyr 500 153 1538 DNA NICOTIANATABACUM 153 caactgcagt ttgaagatac caactaacca aaatgcagtt cttcagcttg gtttccattt 60 tcctatttct atcttttctc tttttgttaa ggaaatggaa gaactcgaat agccaaagga 120 aaaaattgcc accaggtcca tggaaactac caatactagg aagtatgctt catatggttg 180 gtggactacc acaccatgtc cttagagatt tagccaaaaa atatggaccg cttatgcacc 240 ttcaattagg tgaagtttct gcagttgtgg ttacttctcc tgatatggca aaagaagtac 300 taaaaactca tgacatcgct ttcgcgtcta ggcctagcct tttggccccg gagattgtct 360 gttacaatag gtctgatctt gcgttttgcc cctatggcga ttattggaga caaatgcgta 420 aaatatgtgt cttggaagtg ctcagtgcca agaatgttcg gacatatagc tctattaggc 480 gcgatgaagt tcttcgtctc cttaatttta tccggtcatc ttctggtgag cctgttaata 540 ttacggaaag gatctttttg ttcacaagct ccatgacatg tagatcagcg tttgggcaag 600 tattcaagga gcaagacaaa tttatacaac taattaaaga agttatactc ttagcaggag 660 ggtttgatgt ggctgacata ttcccttcat acaagtctct tcatgtgctc agtggaatga 720 agggtaagat tatgaatgca caccataagg tagatgctat tgttgagaat gtcatcaacg 780 agcacaagaa aaatcttgca attgggaaaa ctaatggagc gttaggaggt gaagatttaa 840 ttgatgttct tctaaaactt atgaatgatg gaggccttca atttcctatc accaacgaca 900 acatcaaagc tataatcttt gacatgtttg ctgctggaac agagacttca tcgtcaacaa 960 ttgtgtgggc tatggtggaa atggtgaaaa atccaactgt atttgcgaaa gctcaagcag 1020 aagtaagaga tgcatttaga gaaaaagaaa cttttgatga aaatgatgtg gaggagctaa 1080 actatctaaa gttagtcatt aaagaaactc taagacttca tccaccggtt ccacttttgc 1140 tcccaagaga atgtagggaa gagacaaata taaacggcta cactattcct gtaaagacca 1200 aagtcatggt taatgtttgg gcattgggaa gagatccaaa atattgggat gatgcagaaa 1260 cttttaagcc agagagattt gagcagtgct ctaaggattt tgttggtaat aattttgaat 1320 atcttccatt tggtggtgga aggaggattt gtccagggat ttcgtttggt ttagctaatg 1380 cttatttgcc attggctcaa ttactttatc actttgattg ggaactcccc actggaatca 1440 aaccaagcga cttggacttg actgagttgg ttggagtaac tgccgctaga aaaagtgacc 1500 tttacttggt tgcgactcct tatcaacctc ctcaaaac 1538 154 502 PRT NICOTIANATABACUM 154 Met Gln Phe Phe Ser Leu Val Ser Ile Phe Leu Phe Leu Ser Phe Leu 1 5 10 15 Phe Leu Leu Arg Lys Trp Lys Asn Ser Asn Ser Gln Arg Lys Lys Leu 20 25 30 Pro Pro Gly Pro Trp Lys Leu Pro Ile Leu Gly Ser Met Leu His Met 35 40 45 Val Gly Gly Leu Pro His His Val Leu Arg Asp Leu Ala Lys Lys Tyr 50 55 60 Gly Pro Leu Met His Leu Gln Leu Gly Glu Val Ser Ala Val Val Val 65 70 75 80 Thr Ser Pro Asp Met Ala Lys Glu Val Leu Lys Thr His Asp Ile Ala 85 90 95 Phe Ala Ser Arg Pro Ser Leu Leu Ala Pro Glu Ile Val Cys Tyr Asn 100 105 110 Arg Ser Asp Leu Ala Phe Cys Pro Tyr Gly Asp Tyr Trp Arg Gln Met 115 120 125 Arg Lys Ile Cys Val Leu Glu Val Leu Ser Ala Lys Asn Val Arg Thr 130 135 140 Tyr Ser Ser Ile Arg Arg Asp Glu Val Leu Arg Leu Leu Asn Phe Ile 145 150 155 160 Arg Ser Ser Ser Gly Glu Pro Val Asn Ile Thr Glu Arg Ile Phe Leu 165 170 175 Phe Thr Ser Ser Met Thr Cys Arg Ser Ala Phe Gly Gln Val Phe Lys 180 185 190 Glu Gln Asp Lys Phe Ile Gln Leu Ile Lys Glu Val Ile Leu Leu Ala 195 200 205 Gly Gly Phe Asp Val Ala Asp Ile Phe Pro Ser Tyr Lys Ser Leu His 210 215 220 Val Leu Ser Gly Met Lys Gly Lys Ile Met Asn Ala His His Lys Val 225 230 235 240 Asp Ala Ile Val Glu Asn Val Ile Asn Glu His Lys Lys Asn Leu Ala 245 250 255 Ile Gly Lys Thr Asn Gly Ala Leu Gly Gly Glu Asp Leu Ile Asp Val 260 265 270 Leu Leu Lys Leu Met Asn Asp Gly Gly Leu Gln Phe Pro Ile Thr Asn 275 280 285 Asp Asn Ile Lys Ala Ile Ile Phe Asp Met Phe Ala Ala Gly Thr Glu 290 295 300 Thr Ser Ser Ser Thr Ile Val Trp Ala Met Val Glu Met Val Lys Asn 305 310 315 320 Pro Thr Val Phe Ala Lys Ala Gln Ala Glu Val Arg Asp Ala Phe Arg 325 330 335 Glu Lys Glu Thr Phe Asp Glu Asn Asp Val Glu Glu Leu Asn Tyr Leu 340 345 350 Lys Leu Val Ile Lys Glu Thr Leu Arg Leu His Pro Pro Val Pro Leu 355 360 365 Leu Leu Pro Arg Glu Cys Arg Glu Glu Thr Asn Ile Asn Gly Tyr Thr 370 375 380 Ile Pro Val Lys Thr Lys Val Met Val Asn Val Trp Ala Leu Gly Arg 385 390 395 400 Asp Pro Lys Tyr Trp Asp Asp Ala Glu Thr Phe Lys Pro Glu Arg Phe 405 410 415 Glu Gln Cys Ser Lys Asp Phe Val Gly Asn Asn Phe Glu Tyr Leu Pro 420 425 430 Phe Gly Gly Gly Arg Arg Ile Cys Pro Gly Ile Ser Phe Gly Leu Ala 435 440 445 Asn Ala Tyr Leu Pro Leu Ala Gln Leu Leu Tyr His Phe Asp Trp Glu 450 455 460 Leu Pro Thr Gly Ile Lys Pro Ser Asp Leu Asp Leu Thr Glu Leu Val 465 470 475 480 Gly Val Thr Ala Ala Arg Lys Ser Asp Leu Tyr Leu Val Ala Thr Pro 485 490 495 Tyr Gln Pro Pro Gln Asn 500 155 1530 DNA NICOTIANATABACUM 155 aaaagatgtc ttcattttcc acatcttctg ccacttctaa ttccaaactt ccagttcgag 60 aaatcccagg agactatggt ttcccctttt ttggagccat aaaagataga tatgactact 120 tctacaacct cggcacagac gaattctttc ttaccaaaat gcaaaaatac aactctactg 180 tctttagaac caacatgcca ccaggtccat tcattgctaa aaatcccaaa gtaattgttc 240 tcctcgatgc caaaacattt cccgttcttt tcgacaactc taaagtcgaa aaaatgaacg 300 ttcttgatgg cacgtacgtg ccatctactg atttctatgg cggatatcgc ccgtgtgctt 360 atcttgatcc ttctgagtca actcatgcca cacttaaagg gttcttttta tctttaatct 420 cccagcttca taatcaattt attcctttat ttagaacctc aatttctggt cttttcgcaa 480 atcttgagaa tgagatttcc caaaatggca aagcgaactt caacaatatc agcgacatta 540 tgtcattcga ttttgttttt cgtttgttat gtgacaagac cagtccccat gacacaaatc 600 ttggctctaa tggaccaaaa ctctttgata tatggctgtt gcctcaactt gctccattgt 660 ttagtctagg tctaaaattt gtgccgaact ttctggaaga tttaatgttg catacttttc 720 ccttgccatt ttttctagtg agatcgaatt accagaagct ttatgatgct tttagcaagc 780 atgccgaaag tacactgaat gaagcagaga agaatgggat caaaagagac gaagcatgcc 840 acaacttagt ttttcttgca ggtttcaatg cttatggtgg gatgaaagtt ttattccctg 900 cactgataaa gtgggtcgcc aatggaggaa agagtttaca cactcggctg gcaaatgaaa 960 tcaggacaat tatcaaagaa gaatgtggga ccataactct atcagcaatc aacaagatga 1020 gtttagtaaa atcagtagtg tatgaagtat taagaattga acctccagtt ccattccaat 1080 atggtaaagc caaagaagat atcataatcc aaagccatga ttcaactttc ttagtcaaga 1140 aaggtgaaat gatctttgga tatcagcctt ttgctacaaa agatccaaag atttttgaca 1200 aaccagagga gtttattccg gagaggttca tggccgaagg ggaaaaatta ttaaagtatg 1260 tgtattggtc aaatgcaaga gagacagatg atccaacggt ggacaacaaa caatgcccag 1320 cgaaaaatct tgtcgtgctt ttgtgcaggt tgatgttggt ggaggttttc atgcgttacg 1380 acacattcac agtggagtca acaaagctct ttcttgggtc atcagtaacg ttcacgactc 1440 tggaaaaagc gacatgagtt tcagatatct taattgtagg ctgcaaataa taatgtggtc 1500 attctgcaaa ttattgtact tgtgctgatg 1530 156 483 PRT NICOTIANATABACUM 156 Met Ser Ser Phe Ser Thr Ser Ser Ala Thr Ser Asn Ser Lys Leu Pro 1 5 10 15 Val Arg Glu Ile Pro Gly Asp Tyr Gly Phe Pro Phe Phe Gly Ala Ile 20 25 30 Lys Asp Arg Tyr Asp Tyr Phe Tyr Asn Leu Gly Thr Asp Glu Phe Phe 35 40 45 Leu Thr Lys Met Gln Lys Tyr Asn Ser Thr Val Phe Arg Thr Asn Met 50 55 60 Pro Pro Gly Pro Phe Ile Ala Lys Asn Pro Lys Val Ile Val Leu Leu 65 70 75 80 Asp Ala Lys Thr Phe Pro Val Leu Phe Asp Asn Ser Lys Val Glu Lys 85 90 95 Met Asn Val Leu Asp Gly Thr Tyr Val Pro Ser Thr Asp Phe Tyr Gly 100 105 110 Gly Tyr Arg Pro Cys Ala Tyr Leu Asp Pro Ser Glu Ser Thr His Ala 115 120 125 Thr Leu Lys Gly Phe Phe Leu Ser Leu Ile Ser Gln Leu His Asn Gln 130 135 140 Phe Ile Pro Leu Phe Arg Thr Ser Ile Ser Gly Leu Phe Ala Asn Leu 145 150 155 160 Glu Asn Glu Ile Ser Gln Asn Gly Lys Ala Asn Phe Asn Asn Ile Ser 165 170 175 Asp Ile Met Ser Phe Asp Phe Val Phe Arg Leu Leu Cys Asp Lys Thr 180 185 190 Ser Pro His Asp Thr Asn Leu Gly Ser Asn Gly Pro Lys Leu Phe Asp 195 200 205 Ile Trp Leu Leu Pro Gln Leu Ala Pro Leu Phe Ser Leu Gly Leu Lys 210 215 220 Phe Val Pro Asn Phe Leu Glu Asp Leu Met Leu His Thr Phe Pro Leu 225 230 235 240 Pro Phe Phe Leu Val Arg Ser Asn Tyr Gln Lys Leu Tyr Asp Ala Phe 245 250 255 Ser Lys His Ala Glu Ser Thr Leu Asn Glu Ala Glu Lys Asn Gly Ile 260 265 270 Lys Arg Asp Glu Ala Cys His Asn Leu Val Phe Leu Ala Gly Phe Asn 275 280 285 Ala Tyr Gly Gly Met Lys Val Leu Phe Pro Ala Leu Ile Lys Trp Val 290 295 300 Ala Asn Gly Gly Lys Ser Leu His Thr Arg Leu Ala Asn Glu Ile Arg 305 310 315 320 Thr Ile Ile Lys Glu Glu Cys Gly Thr Ile Thr Leu Ser Ala Ile Asn 325 330 335 Lys Met Ser Leu Val Lys Ser Val Val Tyr Glu Val Leu Arg Ile Glu 340 345 350 Pro Pro Val Pro Phe Gln Tyr Gly Lys Ala Lys Glu Asp Ile Ile Ile 355 360 365 Gln Ser His Asp Ser Thr Phe Leu Val Lys Lys Gly Glu Met Ile Phe 370 375 380 Gly Tyr Gln Pro Phe Ala Thr Lys Asp Pro Lys Ile Phe Asp Lys Pro 385 390 395 400 Glu Glu Phe Ile Pro Glu Arg Phe Met Ala Glu Gly Glu Lys Leu Leu 405 410 415 Lys Tyr Val Tyr Trp Ser Asn Ala Arg Glu Thr Asp Asp Pro Thr Val 420 425 430 Asp Asn Lys Gln Cys Pro Ala Lys Asn Leu Val Val Leu Leu Cys Arg 435 440 445 Leu Met Leu Val Glu Val Phe Met Arg Tyr Asp Thr Phe Thr Val Glu 450 455 460 Ser Thr Lys Leu Phe Leu Gly Ser Ser Val Thr Phe Thr Thr Leu Glu 465 470 475 480 Lys Ala Thr 157 1593 DNA NICOTIANATABACUM 157 ccaaaaatgg agcttcaatc ttctcctttc aatttaattt ctttgttcct cttcttttct 60 tttcatttta ttctagtgaa gaaatggaat gccaaaatcc caaagttacc tccaggtccg 120 tggaggcttc cctttattgg aagcctccat cacttgaagg gaaaacttcc acaccataat 180 cttagagatc tagcgcgaaa atatgggcct ctcatgtact tacaactcgg agaaattcct 240 gtagttgtaa tatcttcgcc acgtgtagca aaagctgtac taaaaactca tgatctcgct 300 tttgcaacta gaccacgatt catgtcctca gacattgtgt tttacaaaag cagggacatc 360 tcttttgccc catttggtga ttactggaga cagatgcgta aaatattgac tcaggaactc 420 ctgagtaaca agatgctcaa gtcatatagc ttaatccgaa aggatgagct ctcgaagctc 480 ctctcatcga ttcgtttgga aacaggttct gcagtgaaca taaatgaaaa gcttctctgg 540 tttacgagct gcatgacctg tagattagcc tttggaaaaa tatgcaatga tcgggatgag 600 ttgatcatgc taattaggga gatattaaca ttatcaggag gatttgatgt gggtgatttg 660 ttcccttcct ggaaattact tcataatatg agcaacatga aagctaggtt gacgaatgta 720 caccacaagt atgatttagt tatggagaac atcatcaatg agcaccaaga gaatcatgca 780 gcagggataa agggtaacaa cgagtttggt ggcgaagata tgatcgatgc tctactgagg 840 gctaaggaga ataatgagct tcaatttcct atcgaaaatg acaacatgaa agcagtaatt 900 ctggacttgt ttattgctgg aactgaaact tcatatactg caattatatg ggcactatca 960 gaattgatga agcacccaag tgtgatggcc aaggcacaag ctgaagtgag aaaagtcttc 1020 aaagaaaatg aaaatttcga cgaaaatgat cttgacaagt tgccatactt aaaatcagtg 1080 attaaagaaa cactaaggat gcaccctcca gttcctttgt tagggcctag agaatgcagg 1140 gaccaaacag agatcgatgg ctacactgta cctattaaag ctagagttat ggttaatgct 1200 tgggcgatag gaagagatcc tgaaagttgg gaagatcctg aaagtttcaa accggagcga 1260 tttgaaaata cttctgttga tcttacagga aatcactatc agttcattcc tttcggttca 1320 ggaagaagaa tgtgtccagg aatgtcgttt ggtttagtta acacagggca tcctttagcc 1380 cagttgctct attgctttga ctggaaactc cctgacaagg ttaatgcaaa tgattttcgc 1440 actactgaaa caagtagagt ttttgcagca agcaaagatg acctctactt gattcccaca 1500 aatcacaggg agcaagaata gcttaattta atggagttct tggaagaatt aaagaagaag 1560 ggctatatag gtgagatttt ttgtatggtt gca 1593 158 504 PRT NICOTIANATABACUM 158 Met Glu Leu Gln Ser Ser Pro Phe Asn Leu Ile Ser Leu Phe Leu Phe 1 5 10 15 Phe Ser Phe His Phe Ile Leu Val Lys Lys Trp Asn Ala Lys Ile Pro 20 25 30 Lys Leu Pro Pro Gly Pro Trp Arg Leu Pro Phe Ile Gly Ser Leu His 35 40 45 His Leu Lys Gly Lys Leu Pro His His Asn Leu Arg Asp Leu Ala Arg 50 55 60 Lys Tyr Gly Pro Leu Met Tyr Leu Gln Leu Gly Glu Ile Pro Val Val 65 70 75 80 Val Ile Ser Ser Pro Arg Val Ala Lys Ala Val Leu Lys Thr His Asp 85 90 95 Leu Ala Phe Ala Thr Arg Pro Arg Phe Met Ser Ser Asp Ile Val Phe 100 105 110 Tyr Lys Ser Arg Asp Ile Ser Phe Ala Pro Phe Gly Asp Tyr Trp Arg 115 120 125 Gln Met Arg Lys Ile Leu Thr Gln Glu Leu Leu Ser Asn Lys Met Leu 130 135 140 Lys Ser Tyr Ser Leu Ile Arg Lys Asp Glu Leu Ser Lys Leu Leu Ser 145 150 155 160 Ser Ile Arg Leu Glu Thr Gly Ser Ala Val Asn Ile Asn Glu Lys Leu 165 170 175 Leu Trp Phe Thr Ser Cys Met Thr Cys Arg Leu Ala Phe Gly Lys Ile 180 185 190 Cys Asn Asp Arg Asp Glu Leu Ile Met Leu Ile Arg Glu Ile Leu Thr 195 200 205 Leu Ser Gly Gly Phe Asp Val Gly Asp Leu Phe Pro Ser Trp Lys Leu 210 215 220 Leu His Asn Met Ser Asn Met Lys Ala Arg Leu Thr Asn Val His His 225 230 235 240 Lys Tyr Asp Leu Val Met Glu Asn Ile Ile Asn Glu His Gln Glu Asn 245 250 255 His Ala Ala Gly Ile Lys Gly Asn Asn Glu Phe Gly Gly Glu Asp Met 260 265 270 Ile Asp Ala Leu Leu Arg Ala Lys Glu Asn Asn Glu Leu Gln Phe Pro 275 280 285 Ile Glu Asn Asp Asn Met Lys Ala Val Ile Leu Asp Leu Phe Ile Ala 290 295 300 Gly Thr Glu Thr Ser Tyr Thr Ala Ile Ile Trp Ala Leu Ser Glu Leu 305 310 315 320 Met Lys His Pro Ser Val Met Ala Lys Ala Gln Ala Glu Val Arg Lys 325 330 335 Val Phe Lys Glu Asn Glu Asn Phe Asp Glu Asn Asp Leu Asp Lys Leu 340 345 350 Pro Tyr Leu Lys Ser Val Ile Lys Glu Thr Leu Arg Met His Pro Pro 355 360 365 Val Pro Leu Leu Gly Pro Arg Glu Cys Arg Asp Gln Thr Glu Ile Asp 370 375 380 Gly Tyr Thr Val Pro Ile Lys Ala Arg Val Met Val Asn Ala Trp Ala 385 390 395 400 Ile Gly Arg Asp Pro Glu Ser Trp Glu Asp Pro Glu Ser Phe Lys Pro 405 410 415 Glu Arg Phe Glu Asn Thr Ser Val Asp Leu Thr Gly Asn His Tyr Gln 420 425 430 Phe Ile Pro Phe Gly Ser Gly Arg Arg Met Cys Pro Gly Met Ser Phe 435 440 445 Gly Leu Val Asn Thr Gly His Pro Leu Ala Gln Leu Leu Tyr Cys Phe 450 455 460 Asp Trp Lys Leu Pro Asp Lys Val Asn Ala Asn Asp Phe Arg Thr Thr 465 470 475 480 Glu Thr Ser Arg Val Phe Ala Ala Ser Lys Asp Asp Leu Tyr Leu Ile 485 490 495 Pro Thr Asn His Arg Glu Gln Glu 500 159 1599 DNA NICOTIANATABACUM 159 cttcttccaa aaatggagct tcaatcttct cctttcaatt taatttcttt gttcctcttc 60 ttttcttttc tttttattct agtgaagaaa tggaatgcca aaatcccaaa gttacctcca 120 ggtccgtgga ggcttccctt tattggaagc ctccatcact tgaagggaaa acttccacac 180 cataatctta gagatctagc gcgaaaatat ggacctctca tgtacttaca actcggagaa 240 attcctgtag ttgtaatatc ttcgccacgt gtagcaaaag ctgtactaaa aactcatgat 300 ctcgcttttg caactagacc acgattcatg tcctcagaca ttgtgtttta caaaagcagg 360 gacatctctt ttgccccatt tggtgattac tggagacaga tgcgtaaaat attgactcag 420 gaactcctga gtaacaagat gctcaagtca tatagcttaa tccgaaagga tgagctctcg 480 aagctcctct catcgattcg tttggaaaca ggttctgcag tgaacataaa tgaaaagctt 540 ctctggttta cgagctgcat gacctgtaga ttagcctttg gaaaaatatg caatgatcgg 600 gatgagttga tcatgctaat tagggagata ttaacattat caggaggatt tgatgtgggt 660 gatttgttcc cttcctggaa attacttcat aatatgagca acatgaaagc taggttgacg 720 aatgtacacc acaagtatga tttagttatg gagaacatca tcaatgagca ccaagagaat 780 catgcagcag ggataaaggg taacaacgag tttggtggcg aagatatgat cgatgctcta 840 ctgagggcta aggagaataa tgagcttcaa tttcctatcg aaaatgacaa catgaaagca 900 gtaattctgg acttgtttat tgctggaact gaaacttcat atactgcaat tatatgggca 960 ctatcagaat tgatgaagca cccaagtgtg atggccaagg cacaagctga agtgagaaaa 1020 gtcttcaaag aaaatgaaaa tttcgacgaa aatgatcttg acaagttgcc atacttaaaa 1080 tcagtgatta aagaaacact aaggatgcac cctccagttc ctttgttagg gcctagagaa 1140 tgcagggacc aaacagagat cgatggctac actgtaccta ttaaagctag agttatggtt 1200 aatgcttggg cgataggaag agatcctgaa agttgggaag atcctgaaag tttcaaaccg 1260 gagcgatttg aaaatacttc tgttgatctt acaggaaatc actatcagtt cattcctttc 1320 ggttcaggaa gaagaatgtg tccaggaatg tcgtttggtt tagttaacac agggcatcct 1380 ttagcccagt tgctctattg ctttgactgg aaactccctg acaaggttaa tgcaaatgat 1440 tttcgcacta ctgaaacaag tagagttttt gcagcaagca aagatgacct ctacttgatt 1500 cccacaaatc acagggagca agaatagctt aatttaatgg agttcttgga agaattaaag 1560 aagaagggct atataggtga gattttttgt atggttgca 1599 160 504 PRT NICOTIANATABACUM 160 Met Glu Leu Gln Ser Ser Pro Phe Asn Leu Ile Ser Leu Phe Leu Phe 1 5 10 15 Phe Ser Phe Leu Phe Ile Leu Val Lys Lys Trp Asn Ala Lys Ile Pro 20 25 30 Lys Leu Pro Pro Gly Pro Trp Arg Leu Pro Phe Ile Gly Ser Leu His 35 40 45 His Leu Lys Gly Lys Leu Pro His His Asn Leu Arg Asp Leu Ala Arg 50 55 60 Lys Tyr Gly Pro Leu Met Tyr Leu Gln Leu Gly Glu Ile Pro Val Val 65 70 75 80 Val Ile Ser Ser Pro Arg Val Ala Lys Ala Val Leu Lys Thr His Asp 85 90 95 Leu Ala Phe Ala Thr Arg Pro Arg Phe Met Ser Ser Asp Ile Val Phe 100 105 110 Tyr Lys Ser Arg Asp Ile Ser Phe Ala Pro Phe Gly Asp Tyr Trp Arg 115 120 125 Gln Met Arg Lys Ile Leu Thr Gln Glu Leu Leu Ser Asn Lys Met Leu 130 135 140 Lys Ser Tyr Ser Leu Ile Arg Lys Asp Glu Leu Ser Lys Leu Leu Ser 145 150 155 160 Ser Ile Arg Leu Glu Thr Gly Ser Ala Val Asn Ile Asn Glu Lys Leu 165 170 175 Leu Trp Phe Thr Ser Cys Met Thr Cys Arg Leu Ala Phe Gly Lys Ile 180 185 190 Cys Asn Asp Arg Asp Glu Leu Ile Met Leu Ile Arg Glu Ile Leu Thr 195 200 205 Leu Ser Gly Gly Phe Asp Val Gly Asp Leu Phe Pro Ser Trp Lys Leu 210 215 220 Leu His Asn Met Ser Asn Met Lys Ala Arg Leu Thr Asn Val His His 225 230 235 240 Lys Tyr Asp Leu Val Met Glu Asn Ile Ile Asn Glu His Gln Glu Asn 245 250 255 His Ala Ala Gly Ile Lys Gly Asn Asn Glu Phe Gly Gly Glu Asp Met 260 265 270 Ile Asp Ala Leu Leu Arg Ala Lys Glu Asn Asn Glu Leu Gln Phe Pro 275 280 285 Ile Glu Asn Asp Asn Met Lys Ala Val Ile Leu Asp Leu Phe Ile Ala 290 295 300 Gly Thr Glu Thr Ser Tyr Thr Ala Ile Ile Trp Ala Leu Ser Glu Leu 305 310 315 320 Met Lys His Pro Ser Val Met Ala Lys Ala Gln Ala Glu Val Arg Lys 325 330 335 Val Phe Lys Glu Asn Glu Asn Phe Asp Glu Asn Asp Leu Asp Lys Leu 340 345 350 Pro Tyr Leu Lys Ser Val Ile Lys Glu Thr Leu Arg Met His Pro Pro 355 360 365 Val Pro Leu Leu Gly Pro Arg Glu Cys Arg Asp Gln Thr Glu Ile Asp 370 375 380 Gly Tyr Thr Val Pro Ile Lys Ala Arg Val Met Val Asn Ala Trp Ala 385 390 395 400 Ile Gly Arg Asp Pro Glu Ser Trp Glu Asp Pro Glu Ser Phe Lys Pro 405 410 415 Glu Arg Phe Glu Asn Thr Ser Val Asp Leu Thr Gly Asn His Tyr Gln 420 425 430 Phe Ile Pro Phe Gly Ser Gly Arg Arg Met Cys Pro Gly Met Ser Phe 435 440 445 Gly Leu Val Asn Thr Gly His Pro Leu Ala Gln Leu Leu Tyr Cys Phe 450 455 460 Asp Trp Lys Leu Pro Asp Lys Val Asn Ala Asn Asp Phe Arg Thr Thr 465 470 475 480 Glu Thr Ser Arg Val Phe Ala Ala Ser Lys Asp Asp Leu Tyr Leu Ile 485 490 495 Pro Thr Asn His Arg Glu Gln Glu 500 161 1564 DNA NICOTIANATABACUM 161 ctttctttct tgtaccgaga tggagtttca acacttggtt tcgttcttgc tattcatctc 60 cttcatcttt cttctaattc aaaaatggag gaaatcgaaa aagctgccac ctggtccgtg 120 gaggctacct attattggaa gtgtgcatca cttgacaagt ggagtaccac atcgagttct 180 cagaaattta tcacaaaaat ttggcccgat catgtacttg cagctcgggg aagttcccac 240 agtagttgta tcctccccac acatggccaa acaaatttta aaaactcatg acctcgcttt 300 tgcatctagg ccagaaatca tgatgggaaa aattatttgc tacgattgta aggacattgc 360 cttttccccg tatggtgatt attggagaca tatgcgtaaa ttgagcacct tggaactact 420 tagtgccaag atggtcaagt ccttcagtcc aattcgtcaa gatgagctct caagtctcct 480 atcatccatt gaatcaatgg gaaatttgcc aatcaactta gtagaaaaac ttttatggtt 540 tatgaatgcc gcgacatgta ggtcagcatt tgggaaagtg tgtaaagatc aaaaagagtt 600 gataacattg attcaacgag cagaatcatt atctggtgga ttcgagctgg ctgatttgtt 660 cccttcgaag aagtttctac atggtattag tgggatgcga tctaaactaa tggaagctcg 720 taacaagata gacgcagtct tggacaacat tatcaatgtg cacagagaga atcgggcaaa 780 tggaaatagt tgtaatggtg agtctggaac tgtagatttc atcgatgttt ttctaagggt 840 catggagagt ggcgaattac catttccgat agaaaatgac aacatcaaag cagttattct 900 tgacatgttc gtagcaggat ctgacacatc atcttcaacc gttatttggg cattaacaga 960 aatgatgaag aatccaaaag tcatggctaa agcacaagct gaagtgagag aagcttttaa 1020 aggaaagaaa gcatgtgatg aggatactga tcttgaaaag cttcattacc taaatttagt 1080 gatcaaagag acactccgat tacaccctcc aactcctcta cttgtcccgc gagaatgcag 1140 ggaggaaaca gagatagaag gattcactat accattgaaa agcaaagtct tggttaacgt 1200 atgggcaatt ggaagagatc ccgagaattg gaaaaatcct gaatgtttta taccagagag 1260 attcgaaaat agttctattg agtttactgg aaatcatttt caacttcttc cgtttggcgc 1320 tggaagacga atttgtccag gaatgcaatt tggtttggct cttgttactc tgccattggc 1380 tcatttgctt cacaattttg attggaaact tcccgaagga attaatgcaa gggatttgga 1440 catgacagag gcaaatggga tatctgctag aagagaaaaa gatctttact tgattgctac 1500 tccttatgta tcacctcttg attaactctg aaattttgct ttaatgctgc ttgcttgctt 1560 cact 1564 162 501 PRT NICOTIANATABACUM 162 Met Glu Phe Gln His Leu Val Ser Phe Leu Leu Phe Ile Ser Phe Ile 1 5 10 15 Phe Leu Leu Ile Gln Lys Trp Arg Lys Ser Lys Lys Leu Pro Pro Gly 20 25 30 Pro Trp Arg Leu Pro Ile Ile Gly Ser Val His His Leu Thr Ser Gly 35 40 45 Val Pro His Arg Val Leu Arg Asn Leu Ser Gln Lys Phe Gly Pro Ile 50 55 60 Met Tyr Leu Gln Leu Gly Glu Val Pro Thr Val Val Val Ser Ser Pro 65 70 75 80 His Met Ala Lys Gln Ile Leu Lys Thr His Asp Leu Ala Phe Ala Ser 85 90 95 Arg Pro Glu Ile Met Met Gly Lys Ile Ile Cys Tyr Asp Cys Lys Asp 100 105 110 Ile Ala Phe Ser Pro Tyr Gly Asp Tyr Trp Arg His Met Arg Lys Leu 115 120 125 Ser Thr Leu Glu Leu Leu Ser Ala Lys Met Val Lys Ser Phe Ser Pro 130 135 140 Ile Arg Gln Asp Glu Leu Ser Ser Leu Leu Ser Ser Ile Glu Ser Met 145 150 155 160 Gly Asn Leu Pro Ile Asn Leu Val Glu Lys Leu Leu Trp Phe Met Asn 165 170 175 Ala Ala Thr Cys Arg Ser Ala Phe Gly Lys Val Cys Lys Asp Gln Lys 180 185 190 Glu Leu Ile Thr Leu Ile Gln Arg Ala Glu Ser Leu Ser Gly Gly Phe 195 200 205 Glu Leu Ala Asp Leu Phe Pro Ser Lys Lys Phe Leu His Gly Ile Ser 210 215 220 Gly Met Arg Ser Lys Leu Met Glu Ala Arg Asn Lys Ile Asp Ala Val 225 230 235 240 Leu Asp Asn Ile Ile Asn Val His Arg Glu Asn Arg Ala Asn Gly Asn 245 250 255 Ser Cys Asn Gly Glu Ser Gly Thr Val Asp Phe Ile Asp Val Phe Leu 260 265 270 Arg Val Met Glu Ser Gly Glu Leu Pro Phe Pro Ile Glu Asn Asp Asn 275 280 285 Ile Lys Ala Val Ile Leu Asp Met Phe Val Ala Gly Ser Asp Thr Ser 290 295 300 Ser Ser Thr Val Ile Trp Ala Leu Thr Glu Met Met Lys Asn Pro Lys 305 310 315 320 Val Met Ala Lys Ala Gln Ala Glu Val Arg Glu Ala Phe Lys Gly Lys 325 330 335 Lys Ala Cys Asp Glu Asp Thr Asp Leu Glu Lys Leu His Tyr Leu Asn 340 345 350 Leu Val Ile Lys Glu Thr Leu Arg Leu His Pro Pro Thr Pro Leu Leu 355 360 365 Val Pro Arg Glu Cys Arg Glu Glu Thr Glu Ile Glu Gly Phe Thr Ile 370 375 380 Pro Leu Lys Ser Lys Val Leu Val Asn Val Trp Ala Ile Gly Arg Asp 385 390 395 400 Pro Glu Asn Trp Lys Asn Pro Glu Cys Phe Ile Pro Glu Arg Phe Glu 405 410 415 Asn Ser Ser Ile Glu Phe Thr Gly Asn His Phe Gln Leu Leu Pro Phe 420 425 430 Gly Ala Gly Arg Arg Ile Cys Pro Gly Met Gln Phe Gly Leu Ala Leu 435 440 445 Val Thr Leu Pro Leu Ala His Leu Leu His Asn Phe Asp Trp Lys Leu 450 455 460 Pro Glu Gly Ile Asn Ala Arg Asp Leu Asp Met Thr Glu Ala Asn Gly 465 470 475 480 Ile Ser Ala Arg Arg Glu Lys Asp Leu Tyr Leu Ile Ala Thr Pro Tyr 485 490 495 Val Ser Pro Leu Asp 500 163 1560 DNA NICOTIANATABACUM 163 ctttcttgta ccgagatgga gtttcaacac ttggtttcgt tcttgctatt catctccttc 60 atctttcttc taattcaaaa atggaggaaa tcgaaaaagc tgccacctgg tccgtggagg 120 ctacctatta ttggaagtgt gcatcacttg acaagtggag taccacatcg agttctcaga 180 aatttatcac aaaaatttgg cccgatcatg tacttgcagc tcggggaagt tcccacagta 240 gttgtatcct ccccacacat ggccaaacaa attttaaaaa ctcatgacct cgcttttgca 300 tctaggccag aaatcatgat gggaaaaatt atttgctacg attgtaagga cattgccttt 360 tccccgtatg gtgattattg gagacatatg cgtaaattga gcaccttgga actacttagt 420 gccaagatgg tcaagtcctt cagtccaatt cgtcaagatg agctctcaag tctcctatca 480 tccattgaat caatgggaaa tttgccaatc aacttagtag aaaaactttt atggtttatg 540 aatgccgcga catgtaggtc agcatttggg aaagtgtgta aagatcaaaa agagttgata 600 acattgattc aacgagcaga atcattatct ggtggattcg agctggctga tttgttccct 660 tcgaagaagt ttctacatgg tattagtggg atgcgatcta aactaatgga agctcgtaac 720 aagatagacg cagtcttgga caacattatc aatgtgcaca gagagaatcg ggcaaatgga 780 aatagttgta atggtgagtc tggaactgta gatttcatcg atgtttttct aagggtcatg 840 gagagtggcg aattaccatt tccgatagaa aatgacaaca tcaaagcagt tattcttgac 900 atgttcgtag caggatctga cacatcatct tcaaccgtta tttgggcatt aacagaaacg 960 atgaagaatc caaaagtcat ggctaaagca caagctgaag tgagagaagc ttttaaagga 1020 aagaaagcat gtgatgagga tactgatctt gaaaagcatc attacctaaa tttagtgatc 1080 aaagagacac tccgattaca ccctccaact cctctacttg tcccgcgaga atgcagggag 1140 gaaacagaga tagaaggatt cactatacca ttgaaaagca aagtcttggt taacgtatgg 1200 gcaattggaa gagatcccga gaattggaaa aatcctgaat gttttatacc agagagattc 1260 gaaaatagtt ctattgagtt tactggaaat cattttcaac ttcttccgtt tggcgctgga 1320 agacgaattt gtccaggaat gcaatttggt ttggctcttg ttactctgcc attggctcat 1380 ttgcttcaca attttgattg gaaacttccc gaaggaatta atgcaaggga tttggacatg 1440 acagaggcaa atgggatatc tgctagaaga gaaaaagatc tttacttgat tgctactcct 1500 tatgtatcac ctcttgatta actctgaaat tttgctttaa tgctgcttgc ttgcttcact 1560 164 501 PRT NICOTIANATABACUM 164 Met Glu Phe Gln His Leu Val Ser Phe Leu Leu Phe Ile Ser Phe Ile 1 5 10 15 Phe Leu Leu Ile Gln Lys Trp Arg Lys Ser Lys Lys Leu Pro Pro Gly 20 25 30 Pro Trp Arg Leu Pro Ile Ile Gly Ser Val His His Leu Thr Ser Gly 35 40 45 Val Pro His Arg Val Leu Arg Asn Leu Ser Gln Lys Phe Gly Pro Ile 50 55 60 Met Tyr Leu Gln Leu Gly Glu Val Pro Thr Val Val Val Ser Ser Pro 65 70 75 80 His Met Ala Lys Gln Ile Leu Lys Thr His Asp Leu Ala Phe Ala Ser 85 90 95 Arg Pro Glu Ile Met Met Gly Lys Ile Ile Cys Tyr Asp Cys Lys Asp 100 105 110 Ile Ala Phe Ser Pro Tyr Gly Asp Tyr Trp Arg His Met Arg Lys Leu 115 120 125 Ser Thr Leu Glu Leu Leu Ser Ala Lys Met Val Lys Ser Phe Ser Pro 130 135 140 Ile Arg Gln Asp Glu Leu Ser Ser Leu Leu Ser Ser Ile Glu Ser Met 145 150 155 160 Gly Asn Leu Pro Ile Asn Leu Val Glu Lys Leu Leu Trp Phe Met Asn 165 170 175 Ala Ala Thr Cys Arg Ser Ala Phe Gly Lys Val Cys Lys Asp Gln Lys 180 185 190 Glu Leu Ile Thr Leu Ile Gln Arg Ala Glu Ser Leu Ser Gly Gly Phe 195 200 205 Glu Leu Ala Asp Leu Phe Pro Ser Lys Lys Phe Leu His Gly Ile Ser 210 215 220 Gly Met Arg Ser Lys Leu Met Glu Ala Arg Asn Lys Ile Asp Ala Val 225 230 235 240 Leu Asp Asn Ile Ile Asn Val His Arg Glu Asn Arg Ala Asn Gly Asn 245 250 255 Ser Cys Asn Gly Glu Ser Gly Thr Val Asp Phe Ile Asp Val Phe Leu 260 265 270 Arg Val Met Glu Ser Gly Glu Leu Pro Phe Pro Ile Glu Asn Asp Asn 275 280 285 Ile Lys Ala Val Ile Leu Asp Met Phe Val Ala Gly Ser Asp Thr Ser 290 295 300 Ser Ser Thr Val Ile Trp Ala Leu Thr Glu Thr Met Lys Asn Pro Lys 305 310 315 320 Val Met Ala Lys Ala Gln Ala Glu Val Arg Glu Ala Phe Lys Gly Lys 325 330 335 Lys Ala Cys Asp Glu Asp Thr Asp Leu Glu Lys His His Tyr Leu Asn 340 345 350 Leu Val Ile Lys Glu Thr Leu Arg Leu His Pro Pro Thr Pro Leu Leu 355 360 365 Val Pro Arg Glu Cys Arg Glu Glu Thr Glu Ile Glu Gly Phe Thr Ile 370 375 380 Pro Leu Lys Ser Lys Val Leu Val Asn Val Trp Ala Ile Gly Arg Asp 385 390 395 400 Pro Glu Asn Trp Lys Asn Pro Glu Cys Phe Ile Pro Glu Arg Phe Glu 405 410 415 Asn Ser Ser Ile Glu Phe Thr Gly Asn His Phe Gln Leu Leu Pro Phe 420 425 430 Gly Ala Gly Arg Arg Ile Cys Pro Gly Met Gln Phe Gly Leu Ala Leu 435 440 445 Val Thr Leu Pro Leu Ala His Leu Leu His Asn Phe Asp Trp Lys Leu 450 455 460 Pro Glu Gly Ile Asn Ala Arg Asp Leu Asp Met Thr Glu Ala Asn Gly 465 470 475 480 Ile Ser Ala Arg Arg Glu Lys Asp Leu Tyr Leu Ile Ala Thr Pro Tyr 485 490 495 Val Ser Pro Leu Asp 500 165 1552 DNA NICOTIANATABACUM 165 caaaaacaaa attccaatgg ttaacatgtt cactccaatt atatacgctc ctctcctttt 60 agctttttac attatcacaa aacatttctt acgcaaactc agaaataatc caccagctcc 120 atttcttact ttccccttta ttggccatct ttatctcttc aaaaaaccac ttcaacgtac 180 cttagccaaa atctccgaac gttatggctc tgttcttcta ctcgaattcg gttcacgaaa 240 agtacttttg gtttcttcac catctgcagc tgaagaatgc ttaacaaaaa acgatattat 300 tttcgcgaat cgtcctcttt tgatggctgg aaaacatctt ggatataatt ttacatcttt 360 ggcttggagt tcgtacggag atcattggag aaatctgcga aggattactt cagttgagat 420 gttttcgact catcgtcttc aaatgctaca tgggattcgt attgatgaag tgaaatctat 480 ggttaagagg ctcaattcct ctgccatagc tgaaaaatct gtggatatga agtctatgtt 540 ttttgagctg atgctcaatg ttatgatgag gacaattgct ggaaaaagat attacggtga 600 gaatgtggag gacattgagg aagctacgag attcaaaggt ttggtgcaag agactttcag 660 gattggcggg gcgacgaata ttggcgactt tttgccggcg ttgaagttat tggtgaggaa 720 attggagaaa agtttaattg tgttgcaaga gaacagagat gagtttatgc aggaattaat 780 taaagattgc agaaaaagaa tggagaaaga aggtactgtt actgattcag aaattgaagg 840 gaacaagaaa tgtttaattg aagttttgtt aacactacaa gaaaatgaac cggaatacta 900 caaagatgaa atcatcagaa gccttatgct tgttctatta tcagctggta cagatacttc 960 agttgggaca atggaatggg ctttatcatt aatgttaaac caccctgaaa ctctgaagaa 1020 agcacaagct gaaattgatg aacatatagg acatgaacgt ttagtggacg agtcggacat 1080 caacaaccta ccttacctac gttgtataat caacgagaca ttccgaatgt accctgcagg 1140 accactacta gtcccacacg agtcgtcaga ggaaaccacc gtaggaggct accgtgtacc 1200 cggaggaacc atgttacttg tgaatttgtg ggcaattcac aatgatccaa agctatggga 1260 tgaaccaaga aagtttaaac cagaaagatt tcaaggacta gatggtgtta gagatggtta 1320 caaaatgatg ccttttggtt ctggacgaag gagttgtcct ggagaaggat tggctgttcg 1380 aatggttgcc ttgtcattgg gatgtattat tcaatgtttt gattggcaac gaatcggcga 1440 agaattggtt gatatgactg aaggaactgg acttactttg cctaaagctc aacctttggt 1500 ggccaagtgt agcccacgac ctaaaatggc taatcttctc tctcagattt ga 1552 166 511 PRT NICOTIANATABACUM 166 Met Val Asn Met Phe Thr Pro Ile Ile Tyr Ala Pro Leu Leu Leu Ala 1 5 10 15 Phe Tyr Ile Ile Thr Lys His Phe Leu Arg Lys Leu Arg Asn Asn Pro 20 25 30 Pro Ala Pro Phe Leu Thr Phe Pro Phe Ile Gly His Leu Tyr Leu Phe 35 40 45 Lys Lys Pro Leu Gln Arg Thr Leu Ala Lys Ile Ser Glu Arg Tyr Gly 50 55 60 Ser Val Leu Leu Leu Glu Phe Gly Ser Arg Lys Val Leu Leu Val Ser 65 70 75 80 Ser Pro Ser Ala Ala Glu Glu Cys Leu Thr Lys Asn Asp Ile Ile Phe 85 90 95 Ala Asn Arg Pro Leu Leu Met Ala Gly Lys His Leu Gly Tyr Asn Phe 100 105 110 Thr Ser Leu Ala Trp Ser Ser Tyr Gly Asp His Trp Arg Asn Leu Arg 115 120 125 Arg Ile Thr Ser Val Glu Met Phe Ser Thr His Arg Leu Gln Met Leu 130 135 140 His Gly Ile Arg Ile Asp Glu Val Lys Ser Met Val Lys Arg Leu Asn 145 150 155 160 Ser Ser Ala Ile Ala Glu Lys Ser Val Asp Met Lys Ser Met Phe Phe 165 170 175 Glu Leu Met Leu Asn Val Met Met Arg Thr Ile Ala Gly Lys Arg Tyr 180 185 190 Tyr Gly Glu Asn Val Glu Asp Ile Glu Glu Ala Thr Arg Phe Lys Gly 195 200 205 Leu Val Gln Glu Thr Phe Arg Ile Gly Gly Ala Thr Asn Ile Gly Asp 210 215 220 Phe Leu Pro Ala Leu Lys Leu Leu Val Arg Lys Leu Glu Lys Ser Leu 225 230 235 240 Ile Val Leu Gln Glu Asn Arg Asp Glu Phe Met Gln Glu Leu Ile Lys 245 250 255 Asp Cys Arg Lys Arg Met Glu Lys Glu Gly Thr Val Thr Asp Ser Glu 260 265 270 Ile Glu Gly Asn Lys Lys Cys Leu Ile Glu Val Leu Leu Thr Leu Gln 275 280 285 Glu Asn Glu Pro Glu Tyr Tyr Lys Asp Glu Ile Ile Arg Ser Leu Met 290 295 300 Leu Val Leu Leu Ser Ala Gly Thr Asp Thr Ser Val Gly Thr Met Glu 305 310 315 320 Trp Ala Leu Ser Leu Met Leu Asn His Pro Glu Thr Leu Lys Lys Ala 325 330 335 Gln Ala Glu Ile Asp Glu His Ile Gly His Glu Arg Leu Val Asp Glu 340 345 350 Ser Asp Ile Asn Asn Leu Pro Tyr Leu Arg Cys Ile Ile Asn Glu Thr 355 360 365 Phe Arg Met Tyr Pro Ala Gly Pro Leu Leu Val Pro His Glu Ser Ser 370 375 380 Glu Glu Thr Thr Val Gly Gly Tyr Arg Val Pro Gly Gly Thr Met Leu 385 390 395 400 Leu Val Asn Leu Trp Ala Ile His Asn Asp Pro Lys Leu Trp Asp Glu 405 410 415 Pro Arg Lys Phe Lys Pro Glu Arg Phe Gln Gly Leu Asp Gly Val Arg 420 425 430 Asp Gly Tyr Lys Met Met Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro 435 440 445 Gly Glu Gly Leu Ala Val Arg Met Val Ala Leu Ser Leu Gly Cys Ile 450 455 460 Ile Gln Cys Phe Asp Trp Gln Arg Ile Gly Glu Glu Leu Val Asp Met 465 470 475 480 Thr Glu Gly Thr Gly Leu Thr Leu Pro Lys Ala Gln Pro Leu Val Ala 485 490 495 Lys Cys Ser Pro Arg Pro Lys Met Ala Asn Leu Leu Ser Gln Ile 500 505 510 167 1675 DNA NICOTIANATABACUM 167 caaaaacaaa attccaatgg ttaacatgtt cactccaatt atatacgctc ctctcctttt 60 agctttttac attatcacaa aacatttctt acgcaaactc agaaataacc caccagctcc 120 atttcttact ttccccttta ttggccatct ttatctcttc aaaaaaccac ttcaacgtac 180 cttagccaaa atctccgaac gttatggctc tgttcttcta ctcgaattcg gttcacgaaa 240 agtacttttg gtttcttcac catctgcagc tgaagaatgc ttaacaaaaa acgatattat 300 tttcgcgaat cgtcctcttt tgatggctgg aaaacatctt ggatataatt ttacttcttt 360 ggcttggagt tcgtacggag atcactggag aaatcttcgt aggattactt cagttgagat 420 gttttcgact catcgtcttc aaatgctaca tggaattcgt attgatgaag tgaaatctat 480 ggttaagagg ctcaattcct ctgccatagc tgaaaaatct gtggatatga agtctatgtt 540 ttttgagctg atgctcaatg ttatgatgag gacaattgct ggaaaaagat attacggtga 600 gaatgtggag gacattgagg aagctacgag attcaaaggt ttggtgcaag agactttcag 660 gattggcggg gcgacgaata ttggcgactt tttgccggcg ttgaagttat tggtgaggaa 720 attggagaaa agtttaattg tgttgcaaga gaacagagat gagtttatgc aggaattaat 780 taaagattgc agaaaaagaa tggagaaaga aggtactgtt actgattcag aaattgaagg 840 gaacaagaaa tgtttaattg aagttttgtt aacactacaa gaaaatgaac cggaatacta 900 caaagatgaa atcatcagaa gccttatgct tgttctatta tcagctggta cagatacttc 960 agttgggaca atggaatggg ctttatcatt aatgttaaac caccctgaaa ctctgaagaa 1020 agcacaagct gaaattgatg aacatatagg acatgaacgt ttagtggacg agtcggacat 1080 caacaaccta ccttacctac gttgtataat caacgagaca ttccgaatgt accctgcagg 1140 accactacta gtcccacacg agtcgtcaga ggaaaccacc gtaggaggct accgtgtacc 1200 cggaggaacc atgttacttg tgaatttgtg ggctattcac aatgatccaa agctatggga 1260 tgaaccaaga aagtttaagc cagaaagatt tgaaggacta gaaggtgtta gagacggtta 1320 caaaatgatg ccttttggtt ctggacgaag gagttgtcct ggagaaggat tggctattcg 1380 aatggttgca ttgtcattgg gatgtattat tcaatgcttt gattggcaac gacttgggga 1440 aggattggtt gataagactg aaggaactgg acttactttg cctaaagctc aacctttagt 1500 ggccaagtgt agcccacgac ctataatggc taatcttctt tctcagattt gaacataatt 1560 ggtttctacc aaacatcccc aaactagaat attattattg gttacatata caatgtaatc 1620 aattttgaac catattatat ctcaatgtat tcctttttaa aaaaaaaaaa aaaaa 1675 168 511 PRT NICOTIANATABACUM 168 Met Val Asn Met Phe Thr Pro Ile Ile Tyr Ala Pro Leu Leu Leu Ala 1 5 10 15 Phe Tyr Ile Ile Thr Lys His Phe Leu Arg Lys Leu Arg Asn Asn Pro 20 25 30 Pro Ala Pro Phe Leu Thr Phe Pro Phe Ile Gly His Leu Tyr Leu Phe 35 40 45 Lys Lys Pro Leu Gln Arg Thr Leu Ala Lys Ile Ser Glu Arg Tyr Gly 50 55 60 Ser Val Leu Leu Leu Glu Phe Gly Ser Arg Lys Val Leu Leu Val Ser 65 70 75 80 Ser Pro Ser Ala Ala Glu Glu Cys Leu Thr Lys Asn Asp Ile Ile Phe 85 90 95 Ala Asn Arg Pro Leu Leu Met Ala Gly Lys His Leu Gly Tyr Asn Phe 100 105 110 Thr Ser Leu Ala Trp Ser Ser Tyr Gly Asp His Trp Arg Asn Leu Arg 115 120 125 Arg Ile Thr Ser Val Glu Met Phe Ser Thr His Arg Leu Gln Met Leu 130 135 140 His Gly Ile Arg Ile Asp Glu Val Lys Ser Met Val Lys Arg Leu Asn 145 150 155 160 Ser Ser Ala Ile Ala Glu Lys Ser Val Asp Met Lys Ser Met Phe Phe 165 170 175 Glu Leu Met Leu Asn Val Met Met Arg Thr Ile Ala Gly Lys Arg Tyr 180 185 190 Tyr Gly Glu Asn Val Glu Asp Ile Glu Glu Ala Thr Arg Phe Lys Gly 195 200 205 Leu Val Gln Glu Thr Phe Arg Ile Gly Gly Ala Thr Asn Ile Gly Asp 210 215 220 Phe Leu Pro Ala Leu Lys Leu Leu Val Arg Lys Leu Glu Lys Ser Leu 225 230 235 240 Ile Val Leu Gln Glu Asn Arg Asp Glu Phe Met Gln Glu Leu Ile Lys 245 250 255 Asp Cys Arg Lys Arg Met Glu Lys Glu Gly Thr Val Thr Asp Ser Glu 260 265 270 Ile Glu Gly Asn Lys Lys Cys Leu Ile Glu Val Leu Leu Thr Leu Gln 275 280 285 Glu Asn Glu Pro Glu Tyr Tyr Lys Asp Glu Ile Ile Arg Ser Leu Met 290 295 300 Leu Val Leu Leu Ser Ala Gly Thr Asp Thr Ser Val Gly Thr Met Glu 305 310 315 320 Trp Ala Leu Ser Leu Met Leu Asn His Pro Glu Thr Leu Lys Lys Ala 325 330 335 Gln Ala Glu Ile Asp Glu His Ile Gly His Glu Arg Leu Val Asp Glu 340 345 350 Ser Asp Ile Asn Asn Leu Pro Tyr Leu Arg Cys Ile Ile Asn Glu Thr 355 360 365 Phe Arg Met Tyr Pro Ala Gly Pro Leu Leu Val Pro His Glu Ser Ser 370 375 380 Glu Glu Thr Thr Val Gly Gly Tyr Arg Val Pro Gly Gly Thr Met Leu 385 390 395 400 Leu Val Asn Leu Trp Ala Ile His Asn Asp Pro Lys Leu Trp Asp Glu 405 410 415 Pro Arg Lys Phe Lys Pro Glu Arg Phe Glu Gly Leu Glu Gly Val Arg 420 425 430 Asp Gly Tyr Lys Met Met Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro 435 440 445 Gly Glu Gly Leu Ala Ile Arg Met Val Ala Leu Ser Leu Gly Cys Ile 450 455 460 Ile Gln Cys Phe Asp Trp Gln Arg Leu Gly Glu Gly Leu Val Asp Lys 465 470 475 480 Thr Glu Gly Thr Gly Leu Thr Leu Pro Lys Ala Gln Pro Leu Val Ala 485 490 495 Lys Cys Ser Pro Arg Pro Ile Met Ala Asn Leu Leu Ser Gln Ile 500 505 510 169 1755 DNA NICOTIANATABACUM 169 caacacgctt actatctcct aaatctccac tcaaaaacaa agaagagaaa gatttaaaac 60 taataattat gaaagagatg gtgcaaaaca atatgagcac ttctcttctt gaaactttac 120 aagctacgcc catgatattc tacttcatcg tccctctctt ctgcttattc cttctctcca 180 aatctcgccg taaacgtttg cctccaggtc caactggctg gcctctcatt ggtaacatga 240 tgatgatgga ccagttaact caccgtggcc ttgccaaact agcccaaaaa tatggtggtg 300 tttttcacct taaaatgggt tatgttcaca aaattgtagt ctctggtcca gacgaagctc 360 gccaagtatt acaggaacac gacatcatat tttcgaaccg tccagcgacc gtagccataa 420 gttacctaac atatgacagg gcagacatgg cttttgctga ctatggactc ttctggcggc 480 agatgagaaa actatgtgta atgaaactct tcagccgcaa acgagctgag tcatgggact 540 cagttcgaga cgaagcggat tccatggtta gaattgtaac aaccaacaca ggcacagctg 600 ttaacttagg tgaacttgtt ttcagtctca ctcgtaatat tatctacaga gctgcttttg 660 gaacttgttc tgaagatgga caaggcgagt tcattaaaat tatgcaagag ttttcgaagc 720 tatttggtgc tttcaatata gctgatttta ttccatggct agggtgggtt ggtaagcaga 780 gtctaaatat tagacttgct aaggctagag cgtcgcttga tgggttcatt gattcgatta 840 ttgatgacca tattattaga aagaaagctt atgttaatgg caaaaatgat ggaggtgatc 900 gagaaactga tatggtggat gagcttttag ctttttacag tgaggaagca aaagtaactg 960 agtccgaaga tttgcagaat gctatcagac ttactaagga taatatcaaa gctatcatca 1020 tggatgtaat gtttggaggg acagaaacag tggcttctgc aatagaatgg gccatggcag 1080 agcttatgag gagtcctgaa gatcttaaaa aggtacaaca agagctggct aacgttgttg 1140 gactcaacag aaaagttgaa gaatctgact ttgaaaaatt aacatactta agatgttgtc 1200 taaaagaaac tctacgactt caccctccaa tccctctcct cctccatgag accgccgagg 1260 aatccaccgt ctccggctac catattccgg caaagtcaca tgttattata aattcatttg 1320 ccattgggcg tgacaaaaat tcatgggaag atcctgaaac ttataaacca tctaggtttc 1380 tcaaagaagg tgtaccagat tttaaaggag gtaattttga gtttatacca tttgggtcgg 1440 gtcggcggtc ttgccccggt atgcaacttg ggctttatgc attggaaatg gctgtggccc 1500 atcttcttca ttgttttact tgggaattgc cagatggtat gaaaccaagt gagcttaaaa 1560 tggatgatat ttttggactc actgctccaa gagctaatcg actcgtggct gtgcctactc 1620 cacgtttgtt gtgtcccctt tattaattga agaaaaaagg tggggctttt acttgcatca 1680 aagagtggtg cttgtgattt ttccaccttt tggttaaata tacgaattat tatgatatac 1740 gaattcttgg gcaca 1755 170 525 PRT NICOTIANATABACUM 170 Met Lys Glu Met Val Gln Asn Asn Met Ser Thr Ser Leu Leu Glu Thr 1 5 10 15 Leu Gln Ala Thr Pro Met Ile Phe Tyr Phe Ile Val Pro Leu Phe Cys 20 25 30 Leu Phe Leu Leu Ser Lys Ser Arg Arg Lys Arg Leu Pro Pro Gly Pro 35 40 45 Thr Gly Trp Pro Leu Ile Gly Asn Met Met Met Met Asp Gln Leu Thr 50 55 60 His Arg Gly Leu Ala Lys Leu Ala Gln Lys Tyr Gly Gly Val Phe His 65 70 75 80 Leu Lys Met Gly Tyr Val His Lys Ile Val Val Ser Gly Pro Asp Glu 85 90 95 Ala Arg Gln Val Leu Gln Glu His Asp Ile Ile Phe Ser Asn Arg Pro 100 105 110 Ala Thr Val Ala Ile Ser Tyr Leu Thr Tyr Asp Arg Ala Asp Met Ala 115 120 125 Phe Ala Asp Tyr Gly Leu Phe Trp Arg Gln Met Arg Lys Leu Cys Val 130 135 140 Met Lys Leu Phe Ser Arg Lys Arg Ala Glu Ser Trp Asp Ser Val Arg 145 150 155 160 Asp Glu Ala Asp Ser Met Val Arg Ile Val Thr Thr Asn Thr Gly Thr 165 170 175 Ala Val Asn Leu Gly Glu Leu Val Phe Ser Leu Thr Arg Asn Ile Ile 180 185 190 Tyr Arg Ala Ala Phe Gly Thr Cys Ser Glu Asp Gly Gln Gly Glu Phe 195 200 205 Ile Lys Ile Met Gln Glu Phe Ser Lys Leu Phe Gly Ala Phe Asn Ile 210 215 220 Ala Asp Phe Ile Pro Trp Leu Gly Trp Val Gly Lys Gln Ser Leu Asn 225 230 235 240 Ile Arg Leu Ala Lys Ala Arg Ala Ser Leu Asp Gly Phe Ile Asp Ser 245 250 255 Ile Ile Asp Asp His Ile Ile Arg Lys Lys Ala Tyr Val Asn Gly Lys 260 265 270 Asn Asp Gly Gly Asp Arg Glu Thr Asp Met Val Asp Glu Leu Leu Ala 275 280 285 Phe Tyr Ser Glu Glu Ala Lys Val Thr Glu Ser Glu Asp Leu Gln Asn 290 295 300 Ala Ile Arg Leu Thr Lys Asp Asn Ile Lys Ala Ile Ile Met Asp Val 305 310 315 320 Met Phe Gly Gly Thr Glu Thr Val Ala Ser Ala Ile Glu Trp Ala Met 325 330 335 Ala Glu Leu Met Arg Ser Pro Glu Asp Leu Lys Lys Val Gln Gln Glu 340 345 350 Leu Ala Asn Val Val Gly Leu Asn Arg Lys Val Glu Glu Ser Asp Phe 355 360 365 Glu Lys Leu Thr Tyr Leu Arg Cys Cys Leu Lys Glu Thr Leu Arg Leu 370 375 380 His Pro Pro Ile Pro Leu Leu Leu His Glu Thr Ala Glu Glu Ser Thr 385 390 395 400 Val Ser Gly Tyr His Ile Pro Ala Lys Ser His Val Ile Ile Asn Ser 405 410 415 Phe Ala Ile Gly Arg Asp Lys Asn Ser Trp Glu Asp Pro Glu Thr Tyr 420 425 430 Lys Pro Ser Arg Phe Leu Lys Glu Gly Val Pro Asp Phe Lys Gly Gly 435 440 445 Asn Phe Glu Phe Ile Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly 450 455 460 Met Gln Leu Gly Leu Tyr Ala Leu Glu Met Ala Val Ala His Leu Leu 465 470 475 480 His Cys Phe Thr Trp Glu Leu Pro Asp Gly Met Lys Pro Ser Glu Leu 485 490 495 Lys Met Asp Asp Ile Phe Gly Leu Thr Ala Pro Arg Ala Asn Arg Leu 500 505 510 Val Ala Val Pro Thr Pro Arg Leu Leu Cys Pro Leu Tyr 515 520 525 171 1684 DNA NICOTIANATABACUM 171 tgtgcttgtg agtgtgggag aaggccttca atatggagat accatattac agcttaaaaa 60 ttgcaatttc ttcatttgca attatctttg tactaagatg ggcatggaaa atcttgaatt 120 atgtgtggtt aaaaccaaaa gaattggaga aatacctcag acagcagggt ttcaaaggaa 180 actcttacaa attcttgttt ggggatatga aagagatgaa gaaaatgggt gaagaagcta 240 tgtctaagcc aatcaatttc tctcatgaca tgatttggcc tagagttatg ccattcatcc 300 acaaaaccat caccaattat ggtaagaatt gtattgtgtg gtttgggcca agaccagcag 360 tcctgatcac agacccggaa cttgtaaagg aggtgctaac gaagaatttc gtctatcaga 420 agccgcttgg caatccactc acaaagttgg cagcaactgg aattgcaggc tatgaaacag 480 ataaatgggc tacacataga aggcttctca atcctgcttt tcaccttgac aagttgaagc 540 atatgctacc tgcattccaa tttactgcta gtgagatgtt gagcaaattg gagaaagttg 600 tttcaccaaa cggaacagag atagatgtgt ggccatattt acaaactttg acaagtgatg 660 ccatttcaag aactgcgttt ggaagtagtt atgaagaagg aagaaagatt tttgaccttc 720 aaaaagaaca actttcacta attctagaag tttcacgcac aatatatatt ccaggatgga 780 ggtttttgcc aacgaaaagg aacaaaagga tgaagcaaat atttaatgaa gtacgagcac 840 tggtatttgg aattattaag aaaaggatga gtatgattga aaatggagaa gcacctgatg 900 atttattggg aatattattg gcatccaatt taaaagaaat ccaacaacat ggaaacaaca 960 agaaatttgg tatgagtatt gatgaggtga ttgaagagtg taaactcttc tattttgctg 1020 ggcaagagac tacttcatct ttacttgtat ggactatgat tttgttgtgc aaatatccta 1080 attggcaaga taaagctaga gaagaggttt tgcaagtgtt tgggagtagg gaagttgact 1140 atgacaagtt gaatcagcta aaaatagtaa ctatgatctt aaacgaggtc ttaaggttgt 1200 atccagcagg atatgtgatt aatcgaatgg taaacaaaga aacaaagtta gggaatttgt 1260 gtttaccagc cggcgtacag ctcgtgttac caacaatgtt gttgcaacat gatactgaaa 1320 tatggggaga tgatgcaatg gagttcaatc cagagagatt tagtgatgga atatccaaag 1380 caacaaaagg aaaacttgtg ttttttccat ttagttgggg tccaagaata tgtattgggc 1440 aaaattttgc tatgttagag gctaaaatgg caatggctat gattctgaaa acctatgcat 1500 ttgaactctc tccatcttat gctcatgctc ctcatccact actacttcaa cctcaatatg 1560 gtgctcaatt aattttgtac aagttgtaga tatggtcaat ttggaacttg ttatggaact 1620 tttatcattg taatcaacca tattgaggga acatggtttg aggttaaatc ctcgtgtgtg 1680 tgtc 1684 172 518 PRT NICOTIANATABACUM 172 Met Glu Ile Pro Tyr Tyr Ser Leu Lys Ile Ala Ile Ser Ser Phe Ala 1 5 10 15 Ile Ile Phe Val Leu Arg Trp Ala Trp Lys Ile Leu Asn Tyr Val Trp 20 25 30 Leu Lys Pro Lys Glu Leu Glu Lys Tyr Leu Arg Gln Gln Gly Phe Lys 35 40 45 Gly Asn Ser Tyr Lys Phe Leu Phe Gly Asp Met Lys Glu Met Lys Lys 50 55 60 Met Gly Glu Glu Ala Met Ser Lys Pro Ile Asn Phe Ser His Asp Met 65 70 75 80 Ile Trp Pro Arg Val Met Pro Phe Ile His Lys Thr Ile Thr Asn Tyr 85 90 95 Gly Lys Asn Cys Ile Val Trp Phe Gly Pro Arg Pro Ala Val Leu Ile 100 105 110 Thr Asp Pro Glu Leu Val Lys Glu Val Leu Thr Lys Asn Phe Val Tyr 115 120 125 Gln Lys Pro Leu Gly Asn Pro Leu Thr Lys Leu Ala Ala Thr Gly Ile 130 135 140 Ala Gly Tyr Glu Thr Asp Lys Trp Ala Thr His Arg Arg Leu Leu Asn 145 150 155 160 Pro Ala Phe His Leu Asp Lys Leu Lys His Met Leu Pro Ala Phe Gln 165 170 175 Phe Thr Ala Ser Glu Met Leu Ser Lys Leu Glu Lys Val Val Ser Pro 180 185 190 Asn Gly Thr Glu Ile Asp Val Trp Pro Tyr Leu Gln Thr Leu Thr Ser 195 200 205 Asp Ala Ile Ser Arg Thr Ala Phe Gly Ser Ser Tyr Glu Glu Gly Arg 210 215 220 Lys Ile Phe Asp Leu Gln Lys Glu Gln Leu Ser Leu Ile Leu Glu Val 225 230 235 240 Ser Arg Thr Ile Tyr Ile Pro Gly Trp Arg Phe Leu Pro Thr Lys Arg 245 250 255 Asn Lys Arg Met Lys Gln Ile Phe Asn Glu Val Arg Ala Leu Val Phe 260 265 270 Gly Ile Ile Lys Lys Arg Met Ser Met Ile Glu Asn Gly Glu Ala Pro 275 280 285 Asp Asp Leu Leu Gly Ile Leu Leu Ala Ser Asn Leu Lys Glu Ile Gln 290 295 300 Gln His Gly Asn Asn Lys Lys Phe Gly Met Ser Ile Asp Glu Val Ile 305 310 315 320 Glu Glu Cys Lys Leu Phe Tyr Phe Ala Gly Gln Glu Thr Thr Ser Ser 325 330 335 Leu Leu Val Trp Thr Met Ile Leu Leu Cys Lys Tyr Pro Asn Trp Gln 340 345 350 Asp Lys Ala Arg Glu Glu Val Leu Gln Val Phe Gly Ser Arg Glu Val 355 360 365 Asp Tyr Asp Lys Leu Asn Gln Leu Lys Ile Val Thr Met Ile Leu Asn 370 375 380 Glu Val Leu Arg Leu Tyr Pro Ala Gly Tyr Val Ile Asn Arg Met Val 385 390 395 400 Asn Lys Glu Thr Lys Leu Gly Asn Leu Cys Leu Pro Ala Gly Val Gln 405 410 415 Leu Val Leu Pro Thr Met Leu Leu Gln His Asp Thr Glu Ile Trp Gly 420 425 430 Asp Asp Ala Met Glu Phe Asn Pro Glu Arg Phe Ser Asp Gly Ile Ser 435 440 445 Lys Ala Thr Lys Gly Lys Leu Val Phe Phe Pro Phe Ser Trp Gly Pro 450 455 460 Arg Ile Cys Ile Gly Gln Asn Phe Ala Met Leu Glu Ala Lys Met Ala 465 470 475 480 Met Ala Met Ile Leu Lys Thr Tyr Ala Phe Glu Leu Ser Pro Ser Tyr 485 490 495 Ala His Ala Pro His Pro Leu Leu Leu Gln Pro Gln Tyr Gly Ala Gln 500 505 510 Leu Ile Leu Tyr Lys Leu 515 173 1697 DNA NICOTIANATABACUM 173 ccagcaccaa gacatggaga attcctgggt agttttagcc ttaacaggcc ttcttacatt 60 agtttttctc tcaaagtttc ttcatagtcc tcgtcgtaaa caaaatcttc caccaggtcc 120 aaaaccatgg cctattgttg gcaatataca tcttcttggt tccacccctc acagatccct 180 tcacgaactt gcaaaaagat acggagattt aatgctacta aagttcggtt cgcgcaatgt 240 ccttatttta tcctccccag atatggctag agaattcttg aaaacaaatg atgccatttg 300 ggcttctcgc cctgagcttg ccgctggtaa atatactgct tataattatt gcgacatgac 360 atgggcacgt tatggaccct tttggagaca agcaaggagg atctatctca acgagatttt 420 caatcctaaa cgtttggatt catttgagta cattcgcata gaggaaaggc ataatttgat 480 ttcacgtctt tttgttctct ctgggaagcc aattcttctt agagaccatt taactcggta 540 cactcttaca agtataagta gaacagtatt gagtggaaaa tattttagcg agtcacctgg 600 ccaaaattca atgataactt tgaaacaatt gcaggatatg cttgataagt ggtttttgct 660 taatggtgtg atcaatattg gggactggat accttggctt gctttcttgg atttgcaggg 720 ttatgtcaag caaatgaagg agttgcatag gaacttcgac aaatttcata actttgtgct 780 agatgatcac aaggctaata ggggagagaa gaactttgtg ccaagagaca tggtcgatgt 840 tttgctgcag caagctgagg atcctaatct tgaggtcaaa ctcaccaatg attgtgtcaa 900 gggtctaatg caggacttat tggctggcgg cacggacacc tcagcaacaa ccgttgaatg 960 ggctttttat gaacttctta gacaacctaa gattatgaag aaagcacaac aagagctaga 1020 ccttgtcatt tcacaggaca gatgggttca agaaaaagat tacactcaac tcccttacat 1080 tgagtcaatc atcaaggaaa cattgaggct tcacccagta agcaccatgc ttccaccgcg 1140 cattgccttg gaggattgtc atgtagcagg ctatgacata cctaaaggta caattttaat 1200 tgtgaacact tggagtattg gaagaaattc acagcattgg gagtcaccag aagaattcct 1260 tccggagagg tttgaaggga agaatattgg tgtcacagga caacattttg cgctcttgcc 1320 atttggcgcg ggccggagaa agtgcccagg atacagtctt gggattcgta taattagggc 1380 aactttagct aacttgttgc atggattcaa ctggagattg cctaatggta tgagtccaga 1440 agacattagc atggaagaga tttatgggct aattacacac cccaaagtcg cacttgacgt 1500 gatgatggag cctcgacttc ccaaccatct ttacaaatag tggataatta aaaccattaa 1560 aatcgttttg ttatatgcat gtctcatatt tgtagtggtc aaaatgtttg ttttctatca 1620 tggatgttca gtgcgaggtt gggaatttca agtcattaac gtgtgaaaat attttaaatt 1680 taaaaaaaaa aaaaaaa 1697 174 508 PRT NICOTIANATABACUM 174 Met Glu Asn Ser Trp Val Val Leu Ala Leu Thr Gly Leu Leu Thr Leu 1 5 10 15 Val Phe Leu Ser Lys Phe Leu His Ser Pro Arg Arg Lys Gln Asn Leu 20 25 30 Pro Pro Gly Pro Lys Pro Trp Pro Ile Val Gly Asn Ile His Leu Leu 35 40 45 Gly Ser Thr Pro His Arg Ser Leu His Glu Leu Ala Lys Arg Tyr Gly 50 55 60 Asp Leu Met Leu Leu Lys Phe Gly Ser Arg Asn Val Leu Ile Leu Ser 65 70 75 80 Ser Pro Asp Met Ala Arg Glu Phe Leu Lys Thr Asn Asp Ala Ile Trp 85 90 95 Ala Ser Arg Pro Glu Leu Ala Ala Gly Lys Tyr Thr Ala Tyr Asn Tyr 100 105 110 Cys Asp Met Thr Trp Ala Arg Tyr Gly Pro Phe Trp Arg Gln Ala Arg 115 120 125 Arg Ile Tyr Leu Asn Glu Ile Phe Asn Pro Lys Arg Leu Asp Ser Phe 130 135 140 Glu Tyr Ile Arg Ile Glu Glu Arg His Asn Leu Ile Ser Arg Leu Phe 145 150 155 160 Val Leu Ser Gly Lys Pro Ile Leu Leu Arg Asp His Leu Thr Arg Tyr 165 170 175 Thr Leu Thr Ser Ile Ser Arg Thr Val Leu Ser Gly Lys Tyr Phe Ser 180 185 190 Glu Ser Pro Gly Gln Asn Ser Met Ile Thr Leu Lys Gln Leu Gln Asp 195 200 205 Met Leu Asp Lys Trp Phe Leu Leu Asn Gly Val Ile Asn Ile Gly Asp 210 215 220 Trp Ile Pro Trp Leu Ala Phe Leu Asp Leu Gln Gly Tyr Val Lys Gln 225 230 235 240 Met Lys Glu Leu His Arg Asn Phe Asp Lys Phe His Asn Phe Val Leu 245 250 255 Asp Asp His Lys Ala Asn Arg Gly Glu Lys Asn Phe Val Pro Arg Asp 260 265 270 Met Val Asp Val Leu Leu Gln Gln Ala Glu Asp Pro Asn Leu Glu Val 275 280 285 Lys Leu Thr Asn Asp Cys Val Lys Gly Leu Met Gln Asp Leu Leu Ala 290 295 300 Gly Gly Thr Asp Thr Ser Ala Thr Thr Val Glu Trp Ala Phe Tyr Glu 305 310 315 320 Leu Leu Arg Gln Pro Lys Ile Met Lys Lys Ala Gln Gln Glu Leu Asp 325 330 335 Leu Val Ile Ser Gln Asp Arg Trp Val Gln Glu Lys Asp Tyr Thr Gln 340 345 350 Leu Pro Tyr Ile Glu Ser Ile Ile Lys Glu Thr Leu Arg Leu His Pro 355 360 365 Val Ser Thr Met Leu Pro Pro Arg Ile Ala Leu Glu Asp Cys His Val 370 375 380 Ala Gly Tyr Asp Ile Pro Lys Gly Thr Ile Leu Ile Val Asn Thr Trp 385 390 395 400 Ser Ile Gly Arg Asn Ser Gln His Trp Glu Ser Pro Glu Glu Phe Leu 405 410 415 Pro Glu Arg Phe Glu Gly Lys Asn Ile Gly Val Thr Gly Gln His Phe 420 425 430 Ala Leu Leu Pro Phe Gly Ala Gly Arg Arg Lys Cys Pro Gly Tyr Ser 435 440 445 Leu Gly Ile Arg Ile Ile Arg Ala Thr Leu Ala Asn Leu Leu His Gly 450 455 460 Phe Asn Trp Arg Leu Pro Asn Gly Met Ser Pro Glu Asp Ile Ser Met 465 470 475 480 Glu Glu Ile Tyr Gly Leu Ile Thr His Pro Lys Val Ala Leu Asp Val 485 490 495 Met Met Glu Pro Arg Leu Pro Asn His Leu Tyr Lys 500 505 175 1631 DNA NICOTIANATABACUM 175 actgttcaaa tcacagtaac agcatcttgt gctgccataa taattactct agtggtgtgt 60 atatggagag tgctgaattg ggtttggttc agaccaaaga agctggaaaa gctactgagg 120 aaacaaggtc tcaaaggcaa ttcctacagg attttgtatg gggatatgaa ggagctttct 180 ggtatgatta aggaagctaa ctccaaaccc atgaatcttt ctgatgatat tgccccaaga 240 ttggtccctt tctttcttga taccatcaag aaatatggga aaaaatcctt tgtatggttg 300 ggtccaaaac cgctggtttt tgtcatggac cccgagctta taaaggaagt attctccaaa 360 aactatctgt atcaaaagcc tcattcaaat ccattaacca agttactggc acaaggactt 420 gtaagccaag aggaagacaa atgggccaaa catagaaaaa tcgtcactcc tgccttccac 480 ctggagaagc taaagcatat gcttccagct ttttgtttga gctgtactga gatgctgagc 540 aaatgggaag acattgttgc agttgagggc tcacatgaga tagatatatg gcctggcctt 600 caacaattaa ctagtgatgt gatctctcgg acagcctttg gcagtagcta tgaagcaggt 660 agaaggatat ttgaacttca aaaggaacaa gctcaatttc ttatggaagc tatacgctcc 720 gtttatattc caggctggag gtttttgcca acaaagagga acagaagaat gaaggaaatt 780 gaaaaggatg ttcaagcctt agttagaggt attattgata aaagagtaaa gtcaatgaaa 840 gcaggagagg tgaataatga ggatctgctt ggtatattgc tggaatctaa ttttaaagaa 900 attgaacagc atggaaacaa ggattttgga atgagcattg aagaagtcat tcaagaatgc 960 aagttattct attttgctgg ccaagaaact acatcagtgt tgcttgtatg gactctaata 1020 ttgctgagca ggcatcagga ttggcaagca ctggccagag aagaggtgtt gcaagtcttt 1080 gggaatcaga aaccagattt tgatggatta aatcgtctaa aaattgttac aatgatcttg 1140 tacgagtctt taaggctcta tcccccagta gtgacactta cccgaaggcc taaggaagac 1200 actgtattag gagatgtatc tctaccagca ggtgtgttaa tctccttacc agtgatctta 1260 ttgcatcacg acgaagagat atggggtaaa gatgcaaaga agttcaagcc agagagattc 1320 agagatggag tctcaagtgc aacaaagggt caagtcactt ttttcccatt tacttggggt 1380 cccagaatat gcattggaca aaattttgcc atgttagaag caaagactac tttggctatg 1440 atcctacaac gcttctcctt tgaactgtct ccatcttatg cacatgctcc tcagtccata 1500 ataactttgc aaccccagta tggtgctcca cttattttgc ataaaatata gtttattact 1560 tgtaagtagt gtctcgtttt atgttaagca tgagtccaaa atgttaaggc ttgtagaact 1620 gcaaaatggg a 1631 176 461 PRT NICOTIANATABACUM 176 Met Lys Glu Leu Ser Gly Met Ile Lys Glu Ala Asn Ser Lys Pro Met 1 5 10 15 Asn Leu Ser Asp Asp Ile Ala Pro Arg Leu Val Pro Phe Phe Leu Asp 20 25 30 Thr Ile Lys Lys Tyr Gly Lys Lys Ser Phe Val Trp Leu Gly Pro Lys 35 40 45 Pro Leu Val Phe Val Met Asp Pro Glu Leu Ile Lys Glu Val Phe Ser 50 55 60 Lys Asn Tyr Leu Tyr Gln Lys Pro His Ser Asn Pro Leu Thr Lys Leu 65 70 75 80 Leu Ala Gln Gly Leu Val Ser Gln Glu Glu Asp Lys Trp Ala Lys His 85 90 95 Arg Lys Ile Val Thr Pro Ala Phe His Leu Glu Lys Leu Lys His Met 100 105 110 Leu Pro Ala Phe Cys Leu Ser Cys Thr Glu Met Leu Ser Lys Trp Glu 115 120 125 Asp Ile Val Ala Val Glu Gly Ser His Glu Ile Asp Ile Trp Pro Gly 130 135 140 Leu Gln Gln Leu Thr Ser Asp Val Ile Ser Arg Thr Ala Phe Gly Ser 145 150 155 160 Ser Tyr Glu Ala Gly Arg Arg Ile Phe Glu Leu Gln Lys Glu Gln Ala 165 170 175 Gln Phe Leu Met Glu Ala Ile Arg Ser Val Tyr Ile Pro Gly Trp Arg 180 185 190 Phe Leu Pro Thr Lys Arg Asn Arg Arg Met Lys Glu Ile Glu Lys Asp 195 200 205 Val Gln Ala Leu Val Arg Gly Ile Ile Asp Lys Arg Val Lys Ser Met 210 215 220 Lys Ala Gly Glu Val Asn Asn Glu Asp Leu Leu Gly Ile Leu Leu Glu 225 230 235 240 Ser Asn Phe Lys Glu Ile Glu Gln His Gly Asn Lys Asp Phe Gly Met 245 250 255 Ser Ile Glu Glu Val Ile Gln Glu Cys Lys Leu Phe Tyr Phe Ala Gly 260 265 270 Gln Glu Thr Thr Ser Val Leu Leu Val Trp Thr Leu Ile Leu Leu Ser 275 280 285 Arg His Gln Asp Trp Gln Ala Leu Ala Arg Glu Glu Val Leu Gln Val 290 295 300 Phe Gly Asn Gln Lys Pro Asp Phe Asp Gly Leu Asn Arg Leu Lys Ile 305 310 315 320 Val Thr Met Ile Leu Tyr Glu Ser Leu Arg Leu Tyr Pro Pro Val Val 325 330 335 Thr Leu Thr Arg Arg Pro Lys Glu Asp Thr Val Leu Gly Asp Val Ser 340 345 350 Leu Pro Ala Gly Val Leu Ile Ser Leu Pro Val Ile Leu Leu His His 355 360 365 Asp Glu Glu Ile Trp Gly Lys Asp Ala Lys Lys Phe Lys Pro Glu Arg 370 375 380 Phe Arg Asp Gly Val Ser Ser Ala Thr Lys Gly Gln Val Thr Phe Phe 385 390 395 400 Pro Phe Thr Trp Gly Pro Arg Ile Cys Ile Gly Gln Asn Phe Ala Met 405 410 415 Leu Glu Ala Lys Thr Thr Leu Ala Met Ile Leu Gln Arg Phe Ser Phe 420 425 430 Glu Leu Ser Pro Ser Tyr Ala His Ala Pro Gln Ser Ile Ile Thr Leu 435 440 445 Gln Pro Gln Tyr Gly Ala Pro Leu Ile Leu His Lys Ile 450 455 460 177 1572 DNA NICOTIANATABACUM 177 atttatctct gaaaatgcaa ttcttcagct tggtttccat tttcctattc ctatctttcc 60 tatttttgtt gaggaaatgg aagaactcca atagccaaag caaaaaattg ccaccaggtc 120 catggaaaat accaatacta ggaagtatgc ttcatatgat tggtggagaa ccgcaccatg 180 tccttagaga tttagccaaa aaagatggac cacttatgca ccttcagtta ggtgaaattt 240 ctgcagttgt ggttacttct agggacatgg caaaagaagt gctaaaaact catgacgtcg 300 tttttgcatc taggcctaaa attgtagcca tggacattat ctgttataac cagtccgaca 360 ttgcctttag cccttatggc gaccactgga gacaaatgcg taaaatttgt gtcatggaac 420 ttctcaatgc aaagaatgtt cggtctttca gctccatcag acgtgatgaa gtcgttcgtc 480 tcattgactc tatccggtca gattcttctt caggtgagct agttaatttt acgcagagga 540 tcatttggtt tgcaagctcc atgacgtgta gatcagcatt tgggcaagta ctcaaggggc 600 aagacatatt tgccaaaaag atcagagaag taataggatt agcagaaggc tttgatgtgg 660 tagacatctt ccctacatac aagtttcttc atgttctcag tgggatgaag cgtaaacttt 720 tgaatgccca ccttaaggta gacgccattg ttgaggatgt catcaacgag cacaagaaaa 780 atcttgcagc tggcaagagt aatggcgcat taggaggcga agatctaatt gatgtcctac 840 tgagacttat gaatgacaca agtcttcaat ttcccatcac caacgacaat atcaaagctg 900 ttgttgttga catgtttgct gccggaacag aaacttcatc aacaacaact gtatgggcca 960 tggctgaaat gatgaagaat ccaagtgtat tcgccaaagc tcaagcagaa gtgcgagaag 1020 cctttaggga caaagtatct tttgatgaaa atgatgtgga ggagctgaaa tacttaaagt 1080 tagtcattaa agaaactttg agacttcatc caccgtctcc acttttggtc ccaagagaat 1140 gcagggaaga tacggatata aacggctaca ctattcctgc aaagaccaaa gttatggtta 1200 atgtttgggc attgggaaga gatccaaaat attgggatga cgcggaaagc tttaagccag 1260 agagatttga gcaatgttct gtagatattt ttggtaataa ttttgagttt cttccctttg 1320 gcgggggacg gagaatttgt cctggaatgt catttggttt agctaatctt tacttaccat 1380 tggctcaatt actctatcac tttgactgga aactcccaac cggaatcaag ccaagagact 1440 tggacttgac cgaattatcg ggaataacta ttgctagaaa gggtgacctt tacttaaatg 1500 ctactcctta tcaaccttct cgagagtaat ttactattgg cataaacatt ttaaatttcc 1560 ttcatcaacc tc 1572 178 504 PRT NICOTIANATABACUM 178 Met Gln Phe Phe Ser Leu Val Ser Ile Phe Leu Phe Leu Ser Phe Leu 1 5 10 15 Phe Leu Leu Arg Lys Trp Lys Asn Ser Asn Ser Gln Ser Lys Lys Leu 20 25 30 Pro Pro Gly Pro Trp Lys Ile Pro Ile Leu Gly Ser Met Leu His Met 35 40 45 Ile Gly Gly Glu Pro His His Val Leu Arg Asp Leu Ala Lys Lys Asp 50 55 60 Gly Pro Leu Met His Leu Gln Leu Gly Glu Ile Ser Ala Val Val Val 65 70 75 80 Thr Ser Arg Asp Met Ala Lys Glu Val Leu Lys Thr His Asp Val Val 85 90 95 Phe Ala Ser Arg Pro Lys Ile Val Ala Met Asp Ile Ile Cys Tyr Asn 100 105 110 Gln Ser Asp Ile Ala Phe Ser Pro Tyr Gly Asp His Trp Arg Gln Met 115 120 125 Arg Lys Ile Cys Val Met Glu Leu Leu Asn Ala Lys Asn Val Arg Ser 130 135 140 Phe Ser Ser Ile Arg Arg Asp Glu Val Val Arg Leu Ile Asp Ser Ile 145 150 155 160 Arg Ser Asp Ser Ser Ser Gly Glu Leu Val Asn Phe Thr Gln Arg Ile 165 170 175 Ile Trp Phe Ala Ser Ser Met Thr Cys Arg Ser Ala Phe Gly Gln Val 180 185 190 Leu Lys Gly Gln Asp Ile Phe Ala Lys Lys Ile Arg Glu Val Ile Gly 195 200 205 Leu Ala Glu Gly Phe Asp Val Val Asp Ile Phe Pro Thr Tyr Lys Phe 210 215 220 Leu His Val Leu Ser Gly Met Lys Arg Lys Leu Leu Asn Ala His Leu 225 230 235 240 Lys Val Asp Ala Ile Val Glu Asp Val Ile Asn Glu His Lys Lys Asn 245 250 255 Leu Ala Ala Gly Lys Ser Asn Gly Ala Leu Gly Gly Glu Asp Leu Ile 260 265 270 Asp Val Leu Leu Arg Leu Met Asn Asp Thr Ser Leu Gln Phe Pro Ile 275 280 285 Thr Asn Asp Asn Ile Lys Ala Val Val Val Asp Met Phe Ala Ala Gly 290 295 300 Thr Glu Thr Ser Ser Thr Thr Thr Val Trp Ala Met Ala Glu Met Met 305 310 315 320 Lys Asn Pro Ser Val Phe Ala Lys Ala Gln Ala Glu Val Arg Glu Ala 325 330 335 Phe Arg Asp Lys Val Ser Phe Asp Glu Asn Asp Val Glu Glu Leu Lys 340 345 350 Tyr Leu Lys Leu Val Ile Lys Glu Thr Leu Arg Leu His Pro Pro Ser 355 360 365 Pro Leu Leu Val Pro Arg Glu Cys Arg Glu Asp Thr Asp Ile Asn Gly 370 375 380 Tyr Thr Ile Pro Ala Lys Thr Lys Val Met Val Asn Val Trp Ala Leu 385 390 395 400 Gly Arg Asp Pro Lys Tyr Trp Asp Asp Ala Glu Ser Phe Lys Pro Glu 405 410 415 Arg Phe Glu Gln Cys Ser Val Asp Ile Phe Gly Asn Asn Phe Glu Phe 420 425 430 Leu Pro Phe Gly Gly Gly Arg Arg Ile Cys Pro Gly Met Ser Phe Gly 435 440 445 Leu Ala Asn Leu Tyr Leu Pro Leu Ala Gln Leu Leu Tyr His Phe Asp 450 455 460 Trp Lys Leu Pro Thr Gly Ile Lys Pro Arg Asp Leu Asp Leu Thr Glu 465 470 475 480 Leu Ser Gly Ile Thr Ile Ala Arg Lys Gly Asp Leu Tyr Leu Asn Ala 485 490 495 Thr Pro Tyr Gln Pro Ser Arg Glu 500 179 1633 DNA NICOTIANATABACUM 179 ataatgcttt ctcccataga agccattgta ggactagtaa ccttcacatt tctcttcttc 60 ttcctatgga caaaaaaatc tcaaaaacct tcaaaaccct taccaccgaa aatccccgga 120 ggatggccgg taatcggcca tcttttccac ttcaatgacg acggcgacga ccgtccatta 180 gctcgaaaac tcggagactt agctgacaaa tacggccccg ttttcacttt tcggctaggc 240 cttccccttg tcttagttgt aagcagttac gaagctgtaa aagactgttt ctctacaaat 300 gacgccattt tttccaatcg tccagctttt ctttacggcg attaccttgg ctacaataat 360 gccatgctat ttttggccaa ttacggacct tactggcgaa aaaatcgaaa attagttatt 420 caggaagttc tctccgctag tcgtctcgaa aaattcaaac acgtgagatt tgcaagaatt 480 caagcgagca ttaagaattt atatactcga attgatggaa attcgagtac gataaattta 540 actgattggt tagaagaatt gaattttggt ctgatcgtga agatgatcgc tggaaaaaat 600 tatgaatccg gtaaaggaga tgaacaagtg gagagattta agaaagcgtt taaggatttt 660 atgattttat caatggagtt tgtgttatgg gatgcatttc caattccatt atttaaatgg 720 gtggattttc aagggcatgt taaggctatg aaaaggactt ttaaagatat agattctgtt 780 tttcagaatt ggttagggga acatattaat aaaagagaaa aaatggaggt taatgcagaa 840 gggaatgaac aagatttcat tgatgtggtg ctttcaaaaa tgagtaatga atatcttggt 900 gaaggttact ctcgtgatac tgtcattaaa gcaacggtgt ttagtttggt cttggatgca 960 gcagacacag ttgctcttca cataaattgg ggaatggcat tattgataaa caatcaaaag 1020 gccttgacga aagcacaaga agagatagac acaaaagttg gtaaggacag atgggtagaa 1080 gagagtgata ttaaggattt ggtatacctc caagctattg ttaaagaagt gttacgatta 1140 tatccaccag gacctttgtt agtaccacac gaaaatgtag aagattgtgt tgttagtgga 1200 tatcacattc ctaaagggac aagattattc gcaaacgtca tgaaactgct acgtgatcct 1260 aaactctggc ctgatcctga tactttcgat ccagagagat tcattgctac tgatattgac 1320 tttcgtggtc agtactataa gtatatcccg tttggttctg gaagacgatc ttgtccaggg 1380 atgacttatg cattgcaagt ggaacactta acaatggcac atttgatcca aggtttcaat 1440 tacagaactc caaatgacga gcccttggat atgaaggaag gtgcaggcat aactatacgt 1500 aaggtaaatc ctgtggaact gataatagcg cctcgcctgg cacctgagct ttattaaaac 1560 ctaagatctt tcatcttggt tgatcattgt ataatactcc taaatggata ttcatttacc 1620 ttttatcaat taa 1633 180 517 PRT NICOTIANATABACUM 180 Met Leu Ser Pro Ile Glu Ala Ile Val Gly Leu Val Thr Phe Thr Phe 1 5 10 15 Leu Phe Phe Phe Leu Trp Thr Lys Lys Ser Gln Lys Pro Ser Lys Pro 20 25 30 Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe 35 40 45 His Phe Asn Asp Asp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly 50 55 60 Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu 65 70 75 80 Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Val Lys Asp Cys Phe 85 90 95 Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr Gly 100 105 110 Asp Tyr Leu Gly Tyr Asn Asn Ala Met Leu Phe Leu Ala Asn Tyr Gly 115 120 125 Pro Tyr Trp Arg Lys Asn Arg Lys Leu Val Ile Gln Glu Val Leu Ser 130 135 140 Ala Ser Arg Leu Glu Lys Phe Lys His Val Arg Phe Ala Arg Ile Gln 145 150 155 160 Ala Ser Ile Lys Asn Leu Tyr Thr Arg Ile Asp Gly Asn Ser Ser Thr 165 170 175 Ile Asn Leu Thr Asp Trp Leu Glu Glu Leu Asn Phe Gly Leu Ile Val 180 185 190 Lys Met Ile Ala Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu Gln 195 200 205 Val Glu Arg Phe Lys Lys Ala Phe Lys Asp Phe Met Ile Leu Ser Met 210 215 220 Glu Phe Val Leu Trp Asp Ala Phe Pro Ile Pro Leu Phe Lys Trp Val 225 230 235 240 Asp Phe Gln Gly His Val Lys Ala Met Lys Arg Thr Phe Lys Asp Ile 245 250 255 Asp Ser Val Phe Gln Asn Trp Leu Gly Glu His Ile Asn Lys Arg Glu 260 265 270 Lys Met Glu Val Asn Ala Glu Gly Asn Glu Gln Asp Phe Ile Asp Val 275 280 285 Val Leu Ser Lys Met Ser Asn Glu Tyr Leu Gly Glu Gly Tyr Ser Arg 290 295 300 Asp Thr Val Ile Lys Ala Thr Val Phe Ser Leu Val Leu Asp Ala Ala 305 310 315 320 Asp Thr Val Ala Leu His Ile Asn Trp Gly Met Ala Leu Leu Ile Asn 325 330 335 Asn Gln Lys Ala Leu Thr Lys Ala Gln Glu Glu Ile Asp Thr Lys Val 340 345 350 Gly Lys Asp Arg Trp Val Glu Glu Ser Asp Ile Lys Asp Leu Val Tyr 355 360 365 Leu Gln Ala Ile Val Lys Glu Val Leu Arg Leu Tyr Pro Pro Gly Pro 370 375 380 Leu Leu Val Pro His Glu Asn Val Glu Asp Cys Val Val Ser Gly Tyr 385 390 395 400 His Ile Pro Lys Gly Thr Arg Leu Phe Ala Asn Val Met Lys Leu Leu 405 410 415 Arg Asp Pro Lys Leu Trp Pro Asp Pro Asp Thr Phe Asp Pro Glu Arg 420 425 430 Phe Ile Ala Thr Asp Ile Asp Phe Arg Gly Gln Tyr Tyr Lys Tyr Ile 435 440 445 Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Met Thr Tyr Ala Leu 450 455 460 Gln Val Glu His Leu Thr Met Ala His Leu Ile Gln Gly Phe Asn Tyr 465 470 475 480 Arg Thr Pro Asn Asp Glu Pro Leu Asp Met Lys Glu Gly Ala Gly Ile 485 490 495 Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile Ile Ala Pro Arg Leu 500 505 510 Ala Pro Glu Leu Tyr 515 181 1585 DNA NICOTIANATABACUM 181 aatccataat gctttctccc atagaagcca ttgtaggact agtaaccttc acatttctct 60 tcttcttcct atggacaaaa aaatctcaaa aaccttcaaa acccttacca ccgaaaatcc 120 ccggaggatg gccggtaatc ggccatcttt tccacttcaa tgacgacggc gacgaccgtc 180 cattagctcg aaaactcgga gacttagctg acaaatacgg ccccgttttc acttttcggc 240 taggccttcc ccttgtctta gttgtaagca gttacgaagc tgtaaaagac tgtttctcta 300 caaatgacgc cattttttcc aatcgtccag cttttcttta cggcgattac cttggctaca 360 ataatgccat gctatttttg gccaattacg gaccttactg gcgaaaaaat cgaaaattag 420 ttattcagga agttctctcc gctagtcgtc tcgaaaaatt caaacacgtg agatttgcaa 480 gaattcaagc gagcattaag aatttatata ctcgaattga tggaaattcg agtacgataa 540 atttaactga ttggttagaa gaattgaatt ttggtctgat cgtgaagatg atcgctggaa 600 aaaattatga atccggtaaa ggagatgaac aagtggagag atttaagaaa gcgtttaagg 660 attttatgat tttatcaatg gagtttgtgt tatgggatgc atttccaatt ccattattta 720 aatgggtgga ttttcaaggg catgttaagg ctatgaaaag gacttttaaa gatatagatt 780 ctgtttttca gaattggtta gaggaacata ttaataaaag agaaaaaatg gaggttaatg 840 cagaagggaa tgaacaagat ttcattgatg tggtgctttc aaaaatgagt aatgaatatc 900 ttggtgaagg ttactctcgt gatactgtca ttaaagcaac ggtgtttagt ttggtcttgg 960 atgcagcaga cacagttgct cttcacataa attggggaat ggcattattg ataaacaatc 1020 aaaaggcctt gacgaaagca caagaagaga tagacacaaa agttggtaag gacagatggg 1080 tagaagagag tgatattaag gatttggtat acctccaagc tattgttaaa gaagtgttac 1140 gattatatcc accaggacct ttgttagtac cacacgaaaa tgtagaagat tgtgttgtta 1200 gtggatatca cattcctaaa gggacaagat tattcgcaaa cgtcatgaaa ctgcaacgtg 1260 atcctaaact ctggtctgat cctgatactt tcgatccaga gagattcatt gctactgata 1320 ttgactttcg tggtcagtac tataagtata tcccgtttgg ttctggaaga cgatcttgtc 1380 cagggatgac ttatgcattg caagtggaac acttaacaat ggcacatttg atccaaggtt 1440 tcaattacag aactccaaat gacgagccct tggatatgaa ggaaggtgca ggcataacta 1500 tacgtaaggt aaatcctgtg gaactgataa tagcgcctcg cctggcacct gagctttatt 1560 aaaacctaag atcatcttgc ttgat 1585 182 517 PRT NICOTIANATABACUM 182 Met Leu Ser Pro Ile Glu Ala Ile Val Gly Leu Val Thr Phe Thr Phe 1 5 10 15 Leu Phe Phe Phe Leu Trp Thr Lys Lys Ser Gln Lys Pro Ser Lys Pro 20 25 30 Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe 35 40 45 His Phe Asn Asp Asp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly 50 55 60 Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu 65 70 75 80 Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Val Lys Asp Cys Phe 85 90 95 Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr Gly 100 105 110 Asp Tyr Leu Gly Tyr Asn Asn Ala Met Leu Phe Leu Ala Asn Tyr Gly 115 120 125 Pro Tyr Trp Arg Lys Asn Arg Lys Leu Val Ile Gln Glu Val Leu Ser 130 135 140 Ala Ser Arg Leu Glu Lys Phe Lys His Val Arg Phe Ala Arg Ile Gln 145 150 155 160 Ala Ser Ile Lys Asn Leu Tyr Thr Arg Ile Asp Gly Asn Ser Ser Thr 165 170 175 Ile Asn Leu Thr Asp Trp Leu Glu Glu Leu Asn Phe Gly Leu Ile Val 180 185 190 Lys Met Ile Ala Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu Gln 195 200 205 Val Glu Arg Phe Lys Lys Ala Phe Lys Asp Phe Met Ile Leu Ser Met 210 215 220 Glu Phe Val Leu Trp Asp Ala Phe Pro Ile Pro Leu Phe Lys Trp Val 225 230 235 240 Asp Phe Gln Gly His Val Lys Ala Met Lys Arg Thr Phe Lys Asp Ile 245 250 255 Asp Ser Val Phe Gln Asn Trp Leu Glu Glu His Ile Asn Lys Arg Glu 260 265 270 Lys Met Glu Val Asn Ala Glu Gly Asn Glu Gln Asp Phe Ile Asp Val 275 280 285 Val Leu Ser Lys Met Ser Asn Glu Tyr Leu Gly Glu Gly Tyr Ser Arg 290 295 300 Asp Thr Val Ile Lys Ala Thr Val Phe Ser Leu Val Leu Asp Ala Ala 305 310 315 320 Asp Thr Val Ala Leu His Ile Asn Trp Gly Met Ala Leu Leu Ile Asn 325 330 335 Asn Gln Lys Ala Leu Thr Lys Ala Gln Glu Glu Ile Asp Thr Lys Val 340 345 350 Gly Lys Asp Arg Trp Val Glu Glu Ser Asp Ile Lys Asp Leu Val Tyr 355 360 365 Leu Gln Ala Ile Val Lys Glu Val Leu Arg Leu Tyr Pro Pro Gly Pro 370 375 380 Leu Leu Val Pro His Glu Asn Val Glu Asp Cys Val Val Ser Gly Tyr 385 390 395 400 His Ile Pro Lys Gly Thr Arg Leu Phe Ala Asn Val Met Lys Leu Gln 405 410 415 Arg Asp Pro Lys Leu Trp Ser Asp Pro Asp Thr Phe Asp Pro Glu Arg 420 425 430 Phe Ile Ala Thr Asp Ile Asp Phe Arg Gly Gln Tyr Tyr Lys Tyr Ile 435 440 445 Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Met Thr Tyr Ala Leu 450 455 460 Gln Val Glu His Leu Thr Met Ala His Leu Ile Gln Gly Phe Asn Tyr 465 470 475 480 Arg Thr Pro Asn Asp Glu Pro Leu Asp Met Lys Glu Gly Ala Gly Ile 485 490 495 Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile Ile Ala Pro Arg Leu 500 505 510 Ala Pro Glu Leu Tyr 515 183 1620 DNA NICOTIANATABACUM 183 ctaaaactcc ataatggttt ctcccgtaga agccattgta ggactagtaa cccttacact 60 tctcttctac ttcctatggc ccaaaaaatt tcaaatacct tcaaaaccat taccaccgaa 120 aattcccgga gggtggccgg taatcggcca tcttttctac ttcgatgatg acggcgacga 180 ccgtccatta gctcgaaaac tcggagactt agctgacaaa tacggcccgg ttttcacttt 240 ccggctaggc cttccgcttg tgttaattgt aagcagttac gaagctgtaa aagactgctt 300 ctctacaaat gacgccattt tctccaatcg tccagctttt ctttacggtg aataccttgg 360 ctacaataat gccatgctat ttttgacaaa atacggacct tattggcgaa aaaatagaaa 420 attagtcatt caggaagttc tctctgctag tcgtctcgaa aaattgaagc acgtgagatt 480 tggtaaaatt caaacgagca ttaagagttt atacactcga attgatggaa attcgagtac 540 gataaatcta actgattggt tagaagaatt gaattttggt ctgatcgtga aaatgatcgc 600 tgggaaaaat tatgaatccg gtaaaggaga tgaacaagtg gagagattta ggaaagcgta 660 taaggatttt ataattttat caatggagtt tgtgttatgg gatgcttttc caattccatt 720 gttcaaatgg gtggattttc aaggctatgt taaggccatg aaaaggacat ttaaggatat 780 agattctgtt tttcagaatt ggttagagga acatgtcaag aaaagagaaa aaatggaggt 840 taatgcacaa gggaatgaac aagatttcat tgatgtggtg ctttcaaaaa tgagtaatga 900 atatcttgat gaaggttact ctcgtgatac tgtcataaaa gcaacagtgt ttagtttggt 960 cttggatgct gcggacacag ttgctcttca catgaattgg ggaatggcat tactgataaa 1020 caatcaacat gccttgaaga aagcacaaga agagatcgat aagaaagttg gtaaggaaag 1080 atgggtagaa gagagtgata ttaaggattt ggtctacctc caagctattg ttaaagaagt 1140 gttacgatta tatccaccag gacctttatt agtacctcat gaaaatgtag aggattgtgt 1200 tgttagtgga tatcacattc ctaaagggac tagactattc gcgaacgtta tgaaattgca 1260 gcgcgatcct aaactctggt caaatcctga taagtttgat ccagagagat tcttcgctga 1320 tgatattgac taccgtggtc agcactatga gtttatccca tttggttctg gaagacgatc 1380 ttgtccgggg atgacttatg cattacaagt ggaacaccta acaatagcac atttgatcca 1440 gggtttcaat tacaaaactc caaatgacga gcccttggat atgaaggaag gtgcaggatt 1500 aactatacgt aaagtaaatc ctgtagaagt gacaattacg gctcgcctgg cacctgagct 1560 ttattaaaac cttagatgtt ttatcttgat tgtactaata tatatatgca gaaaaaattg 1620 184 517 PRT NICOTIANATABACUM 184 Met Val Ser Pro Val Glu Ala Ile Val Gly Leu Val Thr Leu Thr Leu 1 5 10 15 Leu Phe Tyr Phe Leu Trp Pro Lys Lys Phe Gln Ile Pro Ser Lys Pro 20 25 30 Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe 35 40 45 Tyr Phe Asp Asp Asp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly 50 55 60 Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu 65 70 75 80 Pro Leu Val Leu Ile Val Ser Ser Tyr Glu Ala Val Lys Asp Cys Phe 85 90 95 Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr Gly 100 105 110 Glu Tyr Leu Gly Tyr Asn Asn Ala Met Leu Phe Leu Thr Lys Tyr Gly 115 120 125 Pro Tyr Trp Arg Lys Asn Arg Lys Leu Val Ile Gln Glu Val Leu Ser 130 135 140 Ala Ser Arg Leu Glu Lys Leu Lys His Val Arg Phe Gly Lys Ile Gln 145 150 155 160 Thr Ser Ile Lys Ser Leu Tyr Thr Arg Ile Asp Gly Asn Ser Ser Thr 165 170 175 Ile Asn Leu Thr Asp Trp Leu Glu Glu Leu Asn Phe Gly Leu Ile Val 180 185 190 Lys Met Ile Ala Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu Gln 195 200 205 Val Glu Arg Phe Arg Lys Ala Tyr Lys Asp Phe Ile Ile Leu Ser Met 210 215 220 Glu Phe Val Leu Trp Asp Ala Phe Pro Ile Pro Leu Phe Lys Trp Val 225 230 235 240 Asp Phe Gln Gly Tyr Val Lys Ala Met Lys Arg Thr Phe Lys Asp Ile 245 250 255 Asp Ser Val Phe Gln Asn Trp Leu Glu Glu His Val Lys Lys Arg Glu 260 265 270 Lys Met Glu Val Asn Ala Gln Gly Asn Glu Gln Asp Phe Ile Asp Val 275 280 285 Val Leu Ser Lys Met Ser Asn Glu Tyr Leu Asp Glu Gly Tyr Ser Arg 290 295 300 Asp Thr Val Ile Lys Ala Thr Val Phe Ser Leu Val Leu Asp Ala Ala 305 310 315 320 Asp Thr Val Ala Leu His Met Asn Trp Gly Met Ala Leu Leu Ile Asn 325 330 335 Asn Gln His Ala Leu Lys Lys Ala Gln Glu Glu Ile Asp Lys Lys Val 340 345 350 Gly Lys Glu Arg Trp Val Glu Glu Ser Asp Ile Lys Asp Leu Val Tyr 355 360 365 Leu Gln Ala Ile Val Lys Glu Val Leu Arg Leu Tyr Pro Pro Gly Pro 370 375 380 Leu Leu Val Pro His Glu Asn Val Glu Asp Cys Val Val Ser Gly Tyr 385 390 395 400 His Ile Pro Lys Gly Thr Arg Leu Phe Ala Asn Val Met Lys Leu Gln 405 410 415 Arg Asp Pro Lys Leu Trp Ser Asn Pro Asp Lys Phe Asp Pro Glu Arg 420 425 430 Phe Phe Ala Asp Asp Ile Asp Tyr Arg Gly Gln His Tyr Glu Phe Ile 435 440 445 Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Met Thr Tyr Ala Leu 450 455 460 Gln Val Glu His Leu Thr Ile Ala His Leu Ile Gln Gly Phe Asn Tyr 465 470 475 480 Lys Thr Pro Asn Asp Glu Pro Leu Asp Met Lys Glu Gly Ala Gly Leu 485 490 495 Thr Ile Arg Lys Val Asn Pro Val Glu Val Thr Ile Thr Ala Arg Leu 500 505 510 Ala Pro Glu Leu Tyr 515 185 1623 DNA NICOTIANATABACUM 185 cgaggctccc caccaaaaaa tcatttctct cgtctaaaat ggatcttctc ttactagaga 60 agaccttaat tggtcttttc tttgccattt taatcgcttt aattgtctct aaacttcgtt 120 caaagcgttt taagcttcct ccaggaccaa ttccagtacc agtttttggt aattggcttc 180 aagttggtga tgatttaaac cacagaaatc ttactgatta tgccaaaaaa tttggcgatc 240 ttttcttgtt aagaatgggt caacgtaact tagttgttgt gtcatctcct gaattagcta 300 aagaagtttt acacacacaa ggtgttgaat ttggttcaag aacaagaaat gttgtgtttg 360 atatttttac tggaaaaggt caagatatgg tttttactgt atatggtgaa cattggagaa 420 aaatgaggag aattatgact gtaccatttt ttactaataa agttgtgcaa cagtatagag 480 gggggtggga gtttgaggtg gcaagtgtaa ttgaggatgt gaaaaaaaat cctgaatctg 540 ctactaatgg gatcgtatta aggaggagat tacaattaat gatgtataat aatatgttta 600 ggattatgtt tgataggaga tttgagagtg aagatgatcc tttgtttgtt aagcttaagg 660 ctttgaatgg tgaaaggagt agattggctc aaagttttga gtataattat ggtgatttta 720 ttccaatttt gaggcctttt ttgagaggtt atttgaagat ctgtaaagaa gttaaggaga 780 agaggctgca gcttttcaaa gattactttg ttgatgaaag aaagaagctt tcaaatacca 840 agagctcgga cagcaatgcc ctaaaatgtg cgattgatca cattcttgag gctcaacaga 900 agggagagat caatgaggac aacgttcttt acattgttga aaacatcaat gttgctgcaa 960 ttgaaacaac attatggtca attgagtggg gtatcgccga gctagtcaac caccctcaca 1020 tccaaaagaa actgcgcgac gagattgaca cagttcttgg accaggagtg caagtgactg 1080 aaccagacac ccacaagctt ccataccttc aggctgtgat caaggaggca cttcgtctcc 1140 gtatggcaat tcctctatta gtcccacaca tgaaccttca cgacgcaaag cttggcgggt 1200 ttgatattcc agcagagagc aaaatcttgg ttaacgcttg gtggttagct aacaacccgg 1260 ctcattggaa gaaacccgaa gagttcagac ccgagaggtt ctttgaagag gagaagcatg 1320 ttgaggccaa tggcaatgac ttcagatatc ttccgtttgg cgttggtagg aggagctgcc 1380 ctggaattat acttgcattg ccaattcttg gcatcacttt gggacgtttg gttcagaact 1440 ttgagctgtt gcctcctcca ggccagtcga agctcgacac cacagagaaa ggtggacagt 1500 tcagtctcca cattttgaag cattccacca ttgtgttgaa accaaggtct ttctgaactt 1560 tgtgatctta ttaattaagg ggttctgaag aaatttgata gtgttggata ttaagggcga 1620 att 1623 186 505 PRT NICOTIANATABACUM 186 Met Asp Leu Leu Leu Leu Glu Lys Thr Leu Ile Gly Leu Phe Phe Ala 1 5 10 15 Ile Leu Ile Ala Leu Ile Val Ser Lys Leu Arg Ser Lys Arg Phe Lys 20 25 30 Leu Pro Pro Gly Pro Ile Pro Val Pro Val Phe Gly Asn Trp Leu Gln 35 40 45 Val Gly Asp Asp Leu Asn His Arg Asn Leu Thr Asp Tyr Ala Lys Lys 50 55 60 Phe Gly Asp Leu Phe Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val 65 70 75 80 Val Ser Ser Pro Glu Leu Ala Lys Glu Val Leu His Thr Gln Gly Val 85 90 95 Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly 100 105 110 Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp Arg Lys 115 120 125 Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln 130 135 140 Gln Tyr Arg Gly Gly Trp Glu Phe Glu Val Ala Ser Val Ile Glu Asp 145 150 155 160 Val Lys Lys Asn Pro Glu Ser Ala Thr Asn Gly Ile Val Leu Arg Arg 165 170 175 Arg Leu Gln Leu Met Met Tyr Asn Asn Met Phe Arg Ile Met Phe Asp 180 185 190 Arg Arg Phe Glu Ser Glu Asp Asp Pro Leu Phe Val Lys Leu Lys Ala 195 200 205 Leu Asn Gly Glu Arg Ser Arg Leu Ala Gln Ser Phe Glu Tyr Asn Tyr 210 215 220 Gly Asp Phe Ile Pro Ile Leu Arg Pro Phe Leu Arg Gly Tyr Leu Lys 225 230 235 240 Ile Cys Lys Glu Val Lys Glu Lys Arg Leu Gln Leu Phe Lys Asp Tyr 245 250 255 Phe Val Asp Glu Arg Lys Lys Leu Ser Asn Thr Lys Ser Ser Asp Ser 260 265 270 Asn Ala Leu Lys Cys Ala Ile Asp His Ile Leu Glu Ala Gln Gln Lys 275 280 285 Gly Glu Ile Asn Glu Asp Asn Val Leu Tyr Ile Val Glu Asn Ile Asn 290 295 300 Val Ala Ala Ile Glu Thr Thr Leu Trp Ser Ile Glu Trp Gly Ile Ala 305 310 315 320 Glu Leu Val Asn His Pro His Ile Gln Lys Lys Leu Arg Asp Glu Ile 325 330 335 Asp Thr Val Leu Gly Pro Gly Val Gln Val Thr Glu Pro Asp Thr His 340 345 350 Lys Leu Pro Tyr Leu Gln Ala Val Ile Lys Glu Ala Leu Arg Leu Arg 355 360 365 Met Ala Ile Pro Leu Leu Val Pro His Met Asn Leu His Asp Ala Lys 370 375 380 Leu Gly Gly Phe Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn Ala 385 390 395 400 Trp Trp Leu Ala Asn Asn Pro Ala His Trp Lys Lys Pro Glu Glu Phe 405 410 415 Arg Pro Glu Arg Phe Phe Glu Glu Glu Lys His Val Glu Ala Asn Gly 420 425 430 Asn Asp Phe Arg Tyr Leu Pro Phe Gly Val Gly Arg Arg Ser Cys Pro 435 440 445 Gly Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Leu Gly Arg Leu 450 455 460 Val Gln Asn Phe Glu Leu Leu Pro Pro Pro Gly Gln Ser Lys Leu Asp 465 470 475 480 Thr Thr Glu Lys Gly Gly Gln Phe Ser Leu His Ile Leu Lys His Ser 485 490 495 Thr Ile Val Leu Lys Pro Arg Ser Phe 500 505 187 1667 DNA NICOTIANATABACUM 187 caacacgctt actatctcct aaatctccac tcaaaaacaa agaagagaaa gatttaaaac 60 taataattat gaaagagatg gtgcaaaaca atatgagcac ttctcttctt gaaactttac 120 aagctacgcc catgatattc tacttcatcg tccctctctt ctgcttattc cttctctcca 180 aatctcgccg taaacgtttg cctccaggtc caactggctg gcctctcatt ggtaacatga 240 tgatgatgga ccagttaact caccgtggcc ttgccaaact agcccaaaaa tatggtggtg 300 tttttcacct taaaatgggt tatgttcaca aaattgtagt ctctggtcca gacgaagctc 360 gccaagtatt acaggaacac gacatcatat tttcgaaccg tccagcgacc gtagccataa 420 gttacctaac atatgacagg gcagacatgg cttttgctga ctatggactc ttctggcggc 480 agatgagaaa actatgtgta atgaaactct tcagccgcaa acgagctgag tcatgggact 540 cagttcgaga cgaagcggat tccatggtta gaattgtaac aaccaacaca ggcacagctg 600 ttaacttagg tgaacttgtt ttcagtctca ctcgtaatat tatctacaga gctgcttttg 660 gaacttgttc tgaagatgga caaggcgagt tcattgaaat tatgcaagag ttttcgaagc 720 tatttggcgc tttcaatata gctgatttta ttccatggct agggtgggtt ggtaagcaga 780 gtctaaatat tagacttgct aaggctagag cgtcgcttga tgggttcatt gattcgatta 840 ttgatgacca tattattaga aagaaagctt atgttaatgg caaaaatgat ggaggtgatc 900 gagaaactga tatggtggat gagcttttag ctttttacag tgaggaagca aaagtaactg 960 agtccgaaga tttgcagaat gctatcagac ttactaagga tagtatcaaa gctatcatca 1020 tggatgtaat gtttggaggg acagaaacag tggcttctgc aatagaatgg gccatggcag 1080 agcttatgag gagtcctgaa gatcttaaaa aagtacaaca agggctggct aacgttgttg 1140 gactcaacag aaaagttgaa gaatctgact ttgaaaaatt aacatactta agatgttgtc 1200 taaaagaaac tctacgactt caccctccaa tccctctcct cctccatgag accgccgagg 1260 aatccaccgt ctccggctac catattccgg caaagtcaca tgttattata aattcatttg 1320 ccattgggcg tgacaaaaat tcatgggaag atcctgaaac ttataaacca tctaggtttc 1380 tcaaagaagg tgtaccagat tttaaaggag gtaattttga gtttatacca tttgggtcgg 1440 gtcggcggtc ttgccccggt atgcaacttg ggctttatgc attggaaatg gctgtggccc 1500 atcttcttca ttgttttact tgggaattgc cagatggtat gaaaccaagt gagcttaaaa 1560 tggatgatat ttttggactc actgctccaa gagctaatcg actcgtggct gtgcctactc 1620 cacgcttgtt gtgtcccctt tattaattga agaaaaaagg tggggct 1667 188 525 PRT NICOTIANATABACUM 188 Met Lys Glu Met Val Gln Asn Asn Met Ser Thr Ser Leu Leu Glu Thr 1 5 10 15 Leu Gln Ala Thr Pro Met Ile Phe Tyr Phe Ile Val Pro Leu Phe Cys 20 25 30 Leu Phe Leu Leu Ser Lys Ser Arg Arg Lys Arg Leu Pro Pro Gly Pro 35 40 45 Thr Gly Trp Pro Leu Ile Gly Asn Met Met Met Met Asp Gln Leu Thr 50 55 60 His Arg Gly Leu Ala Lys Leu Ala Gln Lys Tyr Gly Gly Val Phe His 65 70 75 80 Leu Lys Met Gly Tyr Val His Lys Ile Val Val Ser Gly Pro Asp Glu 85 90 95 Ala Arg Gln Val Leu Gln Glu His Asp Ile Ile Phe Ser Asn Arg Pro 100 105 110 Ala Thr Val Ala Ile Ser Tyr Leu Thr Tyr Asp Arg Ala Asp Met Ala 115 120 125 Phe Ala Asp Tyr Gly Leu Phe Trp Arg Gln Met Arg Lys Leu Cys Val 130 135 140 Met Lys Leu Phe Ser Arg Lys Arg Ala Glu Ser Trp Asp Ser Val Arg 145 150 155 160 Asp Glu Ala Asp Ser Met Val Arg Ile Val Thr Thr Asn Thr Gly Thr 165 170 175 Ala Val Asn Leu Gly Glu Leu Val Phe Ser Leu Thr Arg Asn Ile Ile 180 185 190 Tyr Arg Ala Ala Phe Gly Thr Cys Ser Glu Asp Gly Gln Gly Glu Phe 195 200 205 Ile Glu Ile Met Gln Glu Phe Ser Lys Leu Phe Gly Ala Phe Asn Ile 210 215 220 Ala Asp Phe Ile Pro Trp Leu Gly Trp Val Gly Lys Gln Ser Leu Asn 225 230 235 240 Ile Arg Leu Ala Lys Ala Arg Ala Ser Leu Asp Gly Phe Ile Asp Ser 245 250 255 Ile Ile Asp Asp His Ile Ile Arg Lys Lys Ala Tyr Val Asn Gly Lys 260 265 270 Asn Asp Gly Gly Asp Arg Glu Thr Asp Met Val Asp Glu Leu Leu Ala 275 280 285 Phe Tyr Ser Glu Glu Ala Lys Val Thr Glu Ser Glu Asp Leu Gln Asn 290 295 300 Ala Ile Arg Leu Thr Lys Asp Ser Ile Lys Ala Ile Ile Met Asp Val 305 310 315 320 Met Phe Gly Gly Thr Glu Thr Val Ala Ser Ala Ile Glu Trp Ala Met 325 330 335 Ala Glu Leu Met Arg Ser Pro Glu Asp Leu Lys Lys Val Gln Gln Gly 340 345 350 Leu Ala Asn Val Val Gly Leu Asn Arg Lys Val Glu Glu Ser Asp Phe 355 360 365 Glu Lys Leu Thr Tyr Leu Arg Cys Cys Leu Lys Glu Thr Leu Arg Leu 370 375 380 His Pro Pro Ile Pro Leu Leu Leu His Glu Thr Ala Glu Glu Ser Thr 385 390 395 400 Val Ser Gly Tyr His Ile Pro Ala Lys Ser His Val Ile Ile Asn Ser 405 410 415 Phe Ala Ile Gly Arg Asp Lys Asn Ser Trp Glu Asp Pro Glu Thr Tyr 420 425 430 Lys Pro Ser Arg Phe Leu Lys Glu Gly Val Pro Asp Phe Lys Gly Gly 435 440 445 Asn Phe Glu Phe Ile Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly 450 455 460 Met Gln Leu Gly Leu Tyr Ala Leu Glu Met Ala Val Ala His Leu Leu 465 470 475 480 His Cys Phe Thr Trp Glu Leu Pro Asp Gly Met Lys Pro Ser Glu Leu 485 490 495 Lys Met Asp Asp Ile Phe Gly Leu Thr Ala Pro Arg Ala Asn Arg Leu 500 505 510 Val Ala Val Pro Thr Pro Arg Leu Leu Cys Pro Leu Tyr 515 520 525 189 1620 DNA NICOTIANATABACUM 189 gggggataag aatatggaga taccatatta cagcttaaaa cttacaattt tttcatttgc 60 aattatcttt gtactaagat gggcatggaa aatcttgaat tatgtgtggt taaaaccaaa 120 agaattggag aaatgcatca gacagcaggg tttcaaagga aactcttaca aattcttgtt 180 tggggatatg aaagagataa agaaaatggg tgaagaagct atgtctaagc caatcaattt 240 ctctcatgac atgatttggc ctagagtcat gcccttcatc cacaaaacca tcaccaatta 300 tggtaagaat tgttttgtgt ggtttgggcc aagaccagca gtcctgatca cagacccgga 360 acttgtaaag gaggtgctaa cgaagaattt cgtttatcag aagccacctg gcactccact 420 cacaaaattg gcagcaactg gaattgcagg ctatgaaaca gataaatggg ctacacatag 480 aaggcttctc aatcctgctt ttcaccttga caagttgaag catatgctac ctgcattcca 540 atttactgct tgtgagatgt tgagcaaatt ggagaaagtt gtctcaccaa atggaacaga 600 gatagatgtg tggccatatc tacaaacttt aacaagtgat gccatttcaa gaactgcttt 660 tggcagtagt tatgaagaag gaagaaagct ttttgaactt caaaaggaac aactttcact 720 aattctagaa gtgtcccgca caatatacat cccaggatgg aggtttttgc caacaaaaag 780 gaacaaaagg atgaagcaaa tatttaatga agtacgagcg ctggtattgg gaattattaa 840 gaaaagattg agtatgattg aaaatggaga agctcctgat gatttattgg gtatattatt 900 ggcatccaat ttaaaagaaa tccaacaaca tggaaataac aagaaatttg gtatgagtat 960 tgatgaggtg attgaagagt gtaaactctt ctattttgcg gggcaagaga caacttcatc 1020 tttacttgta tggactatga ttttgttgtg caaacatcct agttggcaag ataaagctag 1080 agaagaggtt ttgcaagtgt ttggaagtag ggaagttgac tatgacaagt tgaatcagct 1140 aaaaatagta actatgatct taaacgaggt cttaaggttg tatccagcag gatatgcgat 1200 taatcgaatg gtaaccaaag aaacaaagtt agggaattta tgtttaccag ctggggtaca 1260 actcttgtta ccaacaattt tgttgcaaca tgatactgaa atatggggag atgatgcaat 1320 ggagttcaat ccagagagat ttagtgatgg aatatccaaa gcaacaaaag gaaaacttgt 1380 gttctttcca tttagttggg gtccaagaat atgtattggg caaaattttg ctatgttaga 1440 ggccaagatg gcaatggcta tgattctgaa aaactatgca tttgaactct ctccatctta 1500 tgctcatgct cctcatccac tactacttca acctcaatat ggtgctcaat taattttgta 1560 caagttgtag aaatggtcaa tttggaactt gttatggaac ttttatcatc gtaatcaacc 1620 190 518 PRT NICOTIANATABACUM 190 Met Glu Ile Pro Tyr Tyr Ser Leu Lys Leu Thr Ile Phe Ser Phe Ala 1 5 10 15 Ile Ile Phe Val Leu Arg Trp Ala Trp Lys Ile Leu Asn Tyr Val Trp 20 25 30 Leu Lys Pro Lys Glu Leu Glu Lys Cys Ile Arg Gln Gln Gly Phe Lys 35 40 45 Gly Asn Ser Tyr Lys Phe Leu Phe Gly Asp Met Lys Glu Ile Lys Lys 50 55 60 Met Gly Glu Glu Ala Met Ser Lys Pro Ile Asn Phe Ser His Asp Met 65 70 75 80 Ile Trp Pro Arg Val Met Pro Phe Ile His Lys Thr Ile Thr Asn Tyr 85 90 95 Gly Lys Asn Cys Phe Val Trp Phe Gly Pro Arg Pro Ala Val Leu Ile 100 105 110 Thr Asp Pro Glu Leu Val Lys Glu Val Leu Thr Lys Asn Phe Val Tyr 115 120 125 Gln Lys Pro Pro Gly Thr Pro Leu Thr Lys Leu Ala Ala Thr Gly Ile 130 135 140 Ala Gly Tyr Glu Thr Asp Lys Trp Ala Thr His Arg Arg Leu Leu Asn 145 150 155 160 Pro Ala Phe His Leu Asp Lys Leu Lys His Met Leu Pro Ala Phe Gln 165 170 175 Phe Thr Ala Cys Glu Met Leu Ser Lys Leu Glu Lys Val Val Ser Pro 180 185 190 Asn Gly Thr Glu Ile Asp Val Trp Pro Tyr Leu Gln Thr Leu Thr Ser 195 200 205 Asp Ala Ile Ser Arg Thr Ala Phe Gly Ser Ser Tyr Glu Glu Gly Arg 210 215 220 Lys Leu Phe Glu Leu Gln Lys Glu Gln Leu Ser Leu Ile Leu Glu Val 225 230 235 240 Ser Arg Thr Ile Tyr Ile Pro Gly Trp Arg Phe Leu Pro Thr Lys Arg 245 250 255 Asn Lys Arg Met Lys Gln Ile Phe Asn Glu Val Arg Ala Leu Val Leu 260 265 270 Gly Ile Ile Lys Lys Arg Leu Ser Met Ile Glu Asn Gly Glu Ala Pro 275 280 285 Asp Asp Leu Leu Gly Ile Leu Leu Ala Ser Asn Leu Lys Glu Ile Gln 290 295 300 Gln His Gly Asn Asn Lys Lys Phe Gly Met Ser Ile Asp Glu Val Ile 305 310 315 320 Glu Glu Cys Lys Leu Phe Tyr Phe Ala Gly Gln Glu Thr Thr Ser Ser 325 330 335 Leu Leu Val Trp Thr Met Ile Leu Leu Cys Lys His Pro Ser Trp Gln 340 345 350 Asp Lys Ala Arg Glu Glu Val Leu Gln Val Phe Gly Ser Arg Glu Val 355 360 365 Asp Tyr Asp Lys Leu Asn Gln Leu Lys Ile Val Thr Met Ile Leu Asn 370 375 380 Glu Val Leu Arg Leu Tyr Pro Ala Gly Tyr Ala Ile Asn Arg Met Val 385 390 395 400 Thr Lys Glu Thr Lys Leu Gly Asn Leu Cys Leu Pro Ala Gly Val Gln 405 410 415 Leu Leu Leu Pro Thr Ile Leu Leu Gln His Asp Thr Glu Ile Trp Gly 420 425 430 Asp Asp Ala Met Glu Phe Asn Pro Glu Arg Phe Ser Asp Gly Ile Ser 435 440 445 Lys Ala Thr Lys Gly Lys Leu Val Phe Phe Pro Phe Ser Trp Gly Pro 450 455 460 Arg Ile Cys Ile Gly Gln Asn Phe Ala Met Leu Glu Ala Lys Met Ala 465 470 475 480 Met Ala Met Ile Leu Lys Asn Tyr Ala Phe Glu Leu Ser Pro Ser Tyr 485 490 495 Ala His Ala Pro His Pro Leu Leu Leu Gln Pro Gln Tyr Gly Ala Gln 500 505 510 Leu Ile Leu Tyr Lys Leu 515 191 1566 DNA NICOTIANATABACUM 191 gtcctaacta aaaatggaga ttcagttttc taacttagtt gcattcttgc tctttctctc 60 cagcatcttt cttctattca aaaaatggaa aaccagaaaa ctaaatttgc ctcctggtcc 120 atggaaatta ccttttattg gaagtttaca ccatttggct gtggcaggtc cacttcctca 180 ccatggccta aaaaatttag ccaaacgcta tggtcctctt atgcatttac aacttggaca 240 aattcctaca ctcatcatat catcacctca aatggcaaaa gaagtactaa aaactcacga 300 cctcgctttt gccactagac caaagcttgt cgtggccgac atcattcact acgacagcac 360 ggacatagca ttttctccgt acggtgaata ctggagacaa attcgtaaaa tttgcatatt 420 ggaactcttg agtgccaaga tggtcaaatt ttttagctcg attcgccaag atgagctctc 480 gaagatgctc tcatctatac gaacgacacc caatcttaca gtcaatctta ctgacaaaat 540 tttttggttt acgagttcgg taacttgtag atcagcttta gggaagatat gtggtgacca 600 agacaaattg atcattttta tgagggaaat aatatcattg gcaggtggat ttagtattgc 660 tgattttttc cctacatgga aaatgattca tgatattgat ggttcgaaat ctaaactggt 720 gaaagcacat cgtaagattg atgaaatttt gggaaatgtt gttgatgagc acaaaaagaa 780 cagagcagat ggcaagaagg gtaatggtga atttggtggt gaagatttga ttgatgtatt 840 gttaagagtt agagaaagtg gagaagttca aattcctatc acaaatgaca atatcaaatc 900 aatattaatc gacatgttct ctgcgggatc tgaaacatca tcgacgacta taatttgggc 960 attagctgaa atgatgaaga aaccaagtgt tttagcaaag gcacaagctg aagtaaggca 1020 agctttgaag gagaaaaaag gttttcaaca gattgatctt gatgagctaa aatatctcaa 1080 gttagtaatc aaagaaacct taagaatgca ccctccaatt cctctattag ttcctagaga 1140 atgtatggag gatacaaaga ttgatggtta caatatacct ttcaaaacaa gagtcatagt 1200 taatgcatgg gcaatcggac gagatccaga aagttgggat gaccccgaaa gctttatgcc 1260 agagagattt gagaatagtt ctattgactt tcttggaaat catcatcagt ttataccatt 1320 tggtgcagga agaaggattt gtccgggaat gctatttggt ttagctaatg ttggacaacc 1380 tttagctcag ttactttatc acttcgattg gaaactccct aatggacaaa gtcatgagaa 1440 tttcgacatg actgagtcac ctggaatttc tgctacaaga aaggatgatc ttgttttgat 1500 tgccactcct tatgattctt attaagcagt agcagaaata aaaagccggg gcaaacagaa 1560 aaaagt 1566 192 503 PRT NICOTIANATABACUM 192 Met Glu Ile Gln Phe Ser Asn Leu Val Ala Phe Leu Leu Phe Leu Ser 1 5 10 15 Ser Ile Phe Leu Leu Phe Lys Lys Trp Lys Thr Arg Lys Leu Asn Leu 20 25 30 Pro Pro Gly Pro Trp Lys Leu Pro Phe Ile Gly Ser Leu His His Leu 35 40 45 Ala Val Ala Gly Pro Leu Pro His His Gly Leu Lys Asn Leu Ala Lys 50 55 60 Arg Tyr Gly Pro Leu Met His Leu Gln Leu Gly Gln Ile Pro Thr Leu 65 70 75 80 Ile Ile Ser Ser Pro Gln Met Ala Lys Glu Val Leu Lys Thr His Asp 85 90 95 Leu Ala Phe Ala Thr Arg Pro Lys Leu Val Val Ala Asp Ile Ile His 100 105 110 Tyr Asp Ser Thr Asp Ile Ala Phe Ser Pro Tyr Gly Glu Tyr Trp Arg 115 120 125 Gln Ile Arg Lys Ile Cys Ile Leu Glu Leu Leu Ser Ala Lys Met Val 130 135 140 Lys Phe Phe Ser Ser Ile Arg Gln Asp Glu Leu Ser Lys Met Leu Ser 145 150 155 160 Ser Ile Arg Thr Thr Pro Asn Leu Thr Val Asn Leu Thr Asp Lys Ile 165 170 175 Phe Trp Phe Thr Ser Ser Val Thr Cys Arg Ser Ala Leu Gly Lys Ile 180 185 190 Cys Gly Asp Gln Asp Lys Leu Ile Ile Phe Met Arg Glu Ile Ile Ser 195 200 205 Leu Ala Gly Gly Phe Ser Ile Ala Asp Phe Phe Pro Thr Trp Lys Met 210 215 220 Ile His Asp Ile Asp Gly Ser Lys Ser Lys Leu Val Lys Ala His Arg 225 230 235 240 Lys Ile Asp Glu Ile Leu Gly Asn Val Val Asp Glu His Lys Lys Asn 245 250 255 Arg Ala Asp Gly Lys Lys Gly Asn Gly Glu Phe Gly Gly Glu Asp Leu 260 265 270 Ile Asp Val Leu Leu Arg Val Arg Glu Ser Gly Glu Val Gln Ile Pro 275 280 285 Ile Thr Asn Asp Asn Ile Lys Ser Ile Leu Ile Asp Met Phe Ser Ala 290 295 300 Gly Ser Glu Thr Ser Ser Thr Thr Ile Ile Trp Ala Leu Ala Glu Met 305 310 315 320 Met Lys Lys Pro Ser Val Leu Ala Lys Ala Gln Ala Glu Val Arg Gln 325 330 335 Ala Leu Lys Glu Lys Lys Gly Phe Gln Gln Ile Asp Leu Asp Glu Leu 340 345 350 Lys Tyr Leu Lys Leu Val Ile Lys Glu Thr Leu Arg Met His Pro Pro 355 360 365 Ile Pro Leu Leu Val Pro Arg Glu Cys Met Glu Asp Thr Lys Ile Asp 370 375 380 Gly Tyr Asn Ile Pro Phe Lys Thr Arg Val Ile Val Asn Ala Trp Ala 385 390 395 400 Ile Gly Arg Asp Pro Glu Ser Trp Asp Asp Pro Glu Ser Phe Met Pro 405 410 415 Glu Arg Phe Glu Asn Ser Ser Ile Asp Phe Leu Gly Asn His His Gln 420 425 430 Phe Ile Pro Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly Met Leu Phe 435 440 445 Gly Leu Ala Asn Val Gly Gln Pro Leu Ala Gln Leu Leu Tyr His Phe 450 455 460 Asp Trp Lys Leu Pro Asn Gly Gln Ser His Glu Asn Phe Asp Met Thr 465 470 475 480 Glu Ser Pro Gly Ile Ser Ala Thr Arg Lys Asp Asp Leu Val Leu Ile 485 490 495 Ala Thr Pro Tyr Asp Ser Tyr 500 193 1652 DNA NICOTIANATABACUM 193 caactaacaa acacattgag tcctctccca aatcactgat tcaccaccaa aagtaccaac 60 aattcaatgg aaggtacaaa cttgactaca tatgcagcag tatttcttga tactctgttt 120 cttttgttcc tttccaaact tcttcgccag aggaaactca atttacctcc aggcccaaaa 180 ccatggccga tcatcggaaa cttaaacctt attggcaatc ttcctcatcg ctcaatccac 240 gaactctccc tcaagtacgg acccgttatg caactccaat tcgggtcttt ccccgttgta 300 gttggatcct ccgtcgaaat ggctaagatt ttcctcaaat ccatggatat taactttgta 360 ggcaggccta aaacggctgc cggaaaatac acaacgtaca attattccga tattacatgg 420 tctccttacg gaccatattg gcgccaggca cgtaggatgt gcctaacgga attattcagc 480 acgaaacgtc tcgattcata cgagtatatt cgggctgagg agttgcattc tcttctccat 540 aatttgaaca aaatatcagg gaaaccaatt gtgttgaaag attattcgac gacgttgagt 600 ttaaatgtta ttagcaggat ggtactgggg aaaaggtatt tggacgaatc cgagaactcg 660 ttcgtgaatc ctgaggaatt taagaagatg ttggacgaat tgtttttgct aaatggtgta 720 cttaatattg gagattcaat tccatggatt gatttcatgg atttgcaagg ttatgttaag 780 aggatgaaag tagtgagcaa gaaattcgac aagtttttag agcatgttat tgatgagcat 840 aacattagga gaaatggagt ggagaattat gttgctaagg atatggtgga tgttttgttg 900 cagctcgctg atgatccgaa gttggaagtt aagctggaga gacatggagt caaagcattc 960 actcaggata tgctggctgg tggaaccgag agttcagcag tgacagtgga gtgggcaatt 1020 tcagagctgc taaagaagcc ggagattttc aaaaaggcta cagaagaatt ggatcgagta 1080 attgggcaga atagatgggt acaagaaaag gacattccaa atcttcctta catagaggca 1140 atagtcaaag agactatgcg actgcacccc gtggcaccaa tgttggtgcc acgtgagtgt 1200 cgagaagata ttaaggtagc aggctacgac gttcagaaag gaactagggt tctcgtgagt 1260 gtatggacta ttggaagaga ccctacattg tgggacgagc ctgaggtgtt caagccggag 1320 agattccatg aaaggtccat agatgttaaa ggacatgatt atgagctttt gccatttgga 1380 gcggggagaa gaatgtgccc gggttatagc ttggggctca aggtgattca agctagctta 1440 gctaatcttc tacatggatt taactggtca ttgcctgata atatgactcc tgaggacctc 1500 aacatggatg agatttttgg gctctctaca cctaaaaaat ttccacttgc tactgtgatt 1560 gagccaagac tttcaccaaa actttactct gtttgattca gcagttctat ggttccgtca 1620 agatagactt tgttacgttt gaacctgtgc tc 1652 194 509 PRT NICOTIANATABACUM 194 Met Glu Gly Thr Asn Leu Thr Thr Tyr Ala Ala Val Phe Leu Asp Thr 1 5 10 15 Leu Phe Leu Leu Phe Leu Ser Lys Leu Leu Arg Gln Arg Lys Leu Asn 20 25 30 Leu Pro Pro Gly Pro Lys Pro Trp Pro Ile Ile Gly Asn Leu Asn Leu 35 40 45 Ile Gly Asn Leu Pro His Arg Ser Ile His Glu Leu Ser Leu Lys Tyr 50 55 60 Gly Pro Val Met Gln Leu Gln Phe Gly Ser Phe Pro Val Val Val Gly 65 70 75 80 Ser Ser Val Glu Met Ala Lys Ile Phe Leu Lys Ser Met Asp Ile Asn 85 90 95 Phe Val Gly Arg Pro Lys Thr Ala Ala Gly Lys Tyr Thr Thr Tyr Asn 100 105 110 Tyr Ser Asp Ile Thr Trp Ser Pro Tyr Gly Pro Tyr Trp Arg Gln Ala 115 120 125 Arg Arg Met Cys Leu Thr Glu Leu Phe Ser Thr Lys Arg Leu Asp Ser 130 135 140 Tyr Glu Tyr Ile Arg Ala Glu Glu Leu His Ser Leu Leu His Asn Leu 145 150 155 160 Asn Lys Ile Ser Gly Lys Pro Ile Val Leu Lys Asp Tyr Ser Thr Thr 165 170 175 Leu Ser Leu Asn Val Ile Ser Arg Met Val Leu Gly Lys Arg Tyr Leu 180 185 190 Asp Glu Ser Glu Asn Ser Phe Val Asn Pro Glu Glu Phe Lys Lys Met 195 200 205 Leu Asp Glu Leu Phe Leu Leu Asn Gly Val Leu Asn Ile Gly Asp Ser 210 215 220 Ile Pro Trp Ile Asp Phe Met Asp Leu Gln Gly Tyr Val Lys Arg Met 225 230 235 240 Lys Val Val Ser Lys Lys Phe Asp Lys Phe Leu Glu His Val Ile Asp 245 250 255 Glu His Asn Ile Arg Arg Asn Gly Val Glu Asn Tyr Val Ala Lys Asp 260 265 270 Met Val Asp Val Leu Leu Gln Leu Ala Asp Asp Pro Lys Leu Glu Val 275 280 285 Lys Leu Glu Arg His Gly Val Lys Ala Phe Thr Gln Asp Met Leu Ala 290 295 300 Gly Gly Thr Glu Ser Ser Ala Val Thr Val Glu Trp Ala Ile Ser Glu 305 310 315 320 Leu Leu Lys Lys Pro Glu Ile Phe Lys Lys Ala Thr Glu Glu Leu Asp 325 330 335 Arg Val Ile Gly Gln Asn Arg Trp Val Gln Glu Lys Asp Ile Pro Asn 340 345 350 Leu Pro Tyr Ile Glu Ala Ile Val Lys Glu Thr Met Arg Leu His Pro 355 360 365 Val Ala Pro Met Leu Val Pro Arg Glu Cys Arg Glu Asp Ile Lys Val 370 375 380 Ala Gly Tyr Asp Val Gln Lys Gly Thr Arg Val Leu Val Ser Val Trp 385 390 395 400 Thr Ile Gly Arg Asp Pro Thr Leu Trp Asp Glu Pro Glu Val Phe Lys 405 410 415 Pro Glu Arg Phe His Glu Arg Ser Ile Asp Val Lys Gly His Asp Tyr 420 425 430 Glu Leu Leu Pro Phe Gly Ala Gly Arg Arg Met Cys Pro Gly Tyr Ser 435 440 445 Leu Gly Leu Lys Val Ile Gln Ala Ser Leu Ala Asn Leu Leu His Gly 450 455 460 Phe Asn Trp Ser Leu Pro Asp Asn Met Thr Pro Glu Asp Leu Asn Met 465 470 475 480 Asp Glu Ile Phe Gly Leu Ser Thr Pro Lys Lys Phe Pro Leu Ala Thr 485 490 495 Val Ile Glu Pro Arg Leu Ser Pro Lys Leu Tyr Ser Val 500 505 195 1657 DNA NICOTIANATABACUM 195 cttcttcctt cctaactaaa aatggagatt cagttttcta acttagttgc attcttgctc 60 tttctctcca gcatctttct tgtattcaaa aaatggaaaa ccagaaaact aaatttgcct 120 cctggtccat ggaaattacc ttttattgga agtttacacc atttggctgt ggcaggtcca 180 cttcctcacc atggcctaaa aaatttagcc aaacgctatg gtcctcttat gcatttacaa 240 cttggacaaa ttcctacact cgtcatatca tcacctcaaa tggcaaaaga agtactaaaa 300 actcacgacc tcgcttttgc cactagacca aagcttgtcg tggccgacat cattcactac 360 gacagcacgg acatagcatt ttcgccatac ggtgaatact ggagacaaat tcgtaaaatt 420 tgcatattgg aactcttgag tgccaagatg gtcaagtttt ttagctcgat tcgccaagat 480 gagctctcga agatggtttc atctatacga acgacgccca atcttccagt caatcttacc 540 gacaagattt tttggtttac gagttcggta atttgtagat cagctttagg gaagatatgt 600 ggtgaccaag acaaattgat catttttatg agggaaataa tatcattggc aggtggattt 660 agtattgctg attttttccc tacatggaaa atgattcatg atattgatgg ttcaaaatct 720 aaactggtga aggcacatcg taagattgat gaaattttgg aaaatgtggt aaatgagcac 780 aaacagaatc gagcagatgg taaaaagggt aatggtgaat ttggtggaga agatctgatt 840 gatgttttgt taagagttag agaaagtgga gaagttcaaa ttccaatcac agatgacaat 900 atcaaatcaa tattaatcga catgttctct gccggatcgg aaacatcatc gacaactata 960 atttgggcat tagctgaaat gatgaagaaa ccaagtgttt tagcaaaggc acaagctgaa 1020 gtgaggcaag ctttgaaggg gaagaaaatt agttttcaag agattgatat tgataagcta 1080 aagtatttga agttagtgat caaagaaact ttaagaatgc accctccaat tcctctgtta 1140 gtccctagag aatgtatgga agatacaaag attgatggtt acaatatacc tttcaaaaca 1200 agagtcattg ttaatgcatg ggcaattgga cgagatcctc aaagttggga tgatcctgaa 1260 agctttacgc cagagagatt tgagaataat tctattgatt ttcttggaaa tcatcatcaa 1320 tttattccat ttggtgcagg aagaaggatt tgtcctggaa tgctatttgg tttagctaat 1380 gttggacaac ctttagctca gttactttat cacttcgatt ggaaactccc taatggacaa 1440 agtcatgaga atttcgacat gactgagtca cctggaattt ctgctacaag aaaggatgat 1500 cttgttttga ttgccactcc ttatgattct tattaagcag tagcagaaat aaaaagccgg 1560 ggcaaacaga aaaaagtatt gctgcttcta ggtattttct attggataaa tttcaaaatt 1620 catccacaat atttagtgtt tgctagagtt ggttagc 1657 196 504 PRT NICOTIANATABACUM 196 Met Glu Ile Gln Phe Ser Asn Leu Val Ala Phe Leu Leu Phe Leu Ser 1 5 10 15 Ser Ile Phe Leu Val Phe Lys Lys Trp Lys Thr Arg Lys Leu Asn Leu 20 25 30 Pro Pro Gly Pro Trp Lys Leu Pro Phe Ile Gly Ser Leu His His Leu 35 40 45 Ala Val Ala Gly Pro Leu Pro His His Gly Leu Lys Asn Leu Ala Lys 50 55 60 Arg Tyr Gly Pro Leu Met His Leu Gln Leu Gly Gln Ile Pro Thr Leu 65 70 75 80 Val Ile Ser Ser Pro Gln Met Ala Lys Glu Val Leu Lys Thr His Asp 85 90 95 Leu Ala Phe Ala Thr Arg Pro Lys Leu Val Val Ala Asp Ile Ile His 100 105 110 Tyr Asp Ser Thr Asp Ile Ala Phe Ser Pro Tyr Gly Glu Tyr Trp Arg 115 120 125 Gln Ile Arg Lys Ile Cys Ile Leu Glu Leu Leu Ser Ala Lys Met Val 130 135 140 Lys Phe Phe Ser Ser Ile Arg Gln Asp Glu Leu Ser Lys Met Val Ser 145 150 155 160 Ser Ile Arg Thr Thr Pro Asn Leu Pro Val Asn Leu Thr Asp Lys Ile 165 170 175 Phe Trp Phe Thr Ser Ser Val Ile Cys Arg Ser Ala Leu Gly Lys Ile 180 185 190 Cys Gly Asp Gln Asp Lys Leu Ile Ile Phe Met Arg Glu Ile Ile Ser 195 200 205 Leu Ala Gly Gly Phe Ser Ile Ala Asp Phe Phe Pro Thr Trp Lys Met 210 215 220 Ile His Asp Ile Asp Gly Ser Lys Ser Lys Leu Val Lys Ala His Arg 225 230 235 240 Lys Ile Asp Glu Ile Leu Glu Asn Val Val Asn Glu His Lys Gln Asn 245 250 255 Arg Ala Asp Gly Lys Lys Gly Asn Gly Glu Phe Gly Gly Glu Asp Leu 260 265 270 Ile Asp Val Leu Leu Arg Val Arg Glu Ser Gly Glu Val Gln Ile Pro 275 280 285 Ile Thr Asp Asp Asn Ile Lys Ser Ile Leu Ile Asp Met Phe Ser Ala 290 295 300 Gly Ser Glu Thr Ser Ser Thr Thr Ile Ile Trp Ala Leu Ala Glu Met 305 310 315 320 Met Lys Lys Pro Ser Val Leu Ala Lys Ala Gln Ala Glu Val Arg Gln 325 330 335 Ala Leu Lys Gly Lys Lys Ile Ser Phe Gln Glu Ile Asp Ile Asp Lys 340 345 350 Leu Lys Tyr Leu Lys Leu Val Ile Lys Glu Thr Leu Arg Met His Pro 355 360 365 Pro Ile Pro Leu Leu Val Pro Arg Glu Cys Met Glu Asp Thr Lys Ile 370 375 380 Asp Gly Tyr Asn Ile Pro Phe Lys Thr Arg Val Ile Val Asn Ala Trp 385 390 395 400 Ala Ile Gly Arg Asp Pro Gln Ser Trp Asp Asp Pro Glu Ser Phe Thr 405 410 415 Pro Glu Arg Phe Glu Asn Asn Ser Ile Asp Phe Leu Gly Asn His His 420 425 430 Gln Phe Ile Pro Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly Met Leu 435 440 445 Phe Gly Leu Ala Asn Val Gly Gln Pro Leu Ala Gln Leu Leu Tyr His 450 455 460 Phe Asp Trp Lys Leu Pro Asn Gly Gln Ser His Glu Asn Phe Asp Met 465 470 475 480 Thr Glu Ser Pro Gly Ile Ser Ala Thr Arg Lys Asp Asp Leu Val Leu 485 490 495 Ile Ala Thr Pro Tyr Asp Ser Tyr 500 197 1610 DNA NICOTIANATABACUM 197 cttcttcctt cctaactaaa aatggagatt cagttttcta acttagttgc attcttgctc 60 tttctctcca gcatctttct tgtattcaaa aaatggaaaa ccagaaaact aaatttgcct 120 cctggtccat ggaaattacc ttttattgga agtttacacc atttggctgt ggcaggtcca 180 cttcctcacc atggcctaaa aaatttagcc aaacgctatg gtcctcttat gcatttacaa 240 cttggacaaa ttcctacact cgtcatatca tcacctcaaa tggcaaaaga agtactaaaa 300 actcacgacc tcgcttttgc cactagacca aagcttgtcg tggccgacat cattcactac 360 gacagcacgg acatagcact ttcgccatac ggtgaatact ggagacaaat tcgtaaaatt 420 tgcatattgg aactcttgag tgccaagatg gtcaagtttt ttagctcgat tcgccaagat 480 gagctctcga agatggtttc atctatacga acgacgccca atcttccagt caatcttacc 540 gacaagattt tttggtttac gagttcggta atttgtagat cagctttagg gaagatatgt 600 ggtgaccaag acaaattgat catttttatg agggaaataa tatcattggc aggtggattt 660 agtattgctg attttttccc tacatggaaa atgattcatg atattgatgg ttcaaaatct 720 aaactggtga aggcacatcg taagattgat gaaattttgg aaaatgtggt aaatgagcac 780 aaacagaatc gagcagatgg taaaaagggt aatggtgaat ttggtggaga agatctgatt 840 gatgttttgt taagagttag agaaagtgga gaagttcaaa ttccaatcac agatgacaat 900 atcaaatcaa tattaatcga catgttctct gccggatcgg aaacatcatc gacaactata 960 atttgggcat tagctgaaat gatgaagaaa ccaagtgttt tagcaaaggc acaagctgaa 1020 gtgagccaag ctttgaaggg gaagaaaatt agttttcaag agattgatat tgataagcta 1080 aagtatttga agttagtgat caaagaaact ttaagaatgc accctccaat tcctctgtta 1140 gtccctagag aatgtatgga agatacaaag attgatggtt acaatatacc tttcaaaaca 1200 agagtcattg ttaatgcatg ggcaattgga cgagatcctc aaagttggga tgatcctgaa 1260 agctttacgc cagagagatt tgagaataat tctattgatt ttcttggaaa tcatcatcaa 1320 tttattccat ttggtgcagg aagaaggatt tgtcctggaa tgctatttgg tttagctaat 1380 gttggacaac ctttagctca gttactttat cacttcgatt ggaaactccc taatggacaa 1440 actcaccaaa atttcgacat gactgagtca cctggaattt ctgctacaag aaaggatgat 1500 cttattttga ttgccactcc tgctcattct tgattaagta ttgctgcttt tctattggag 1560 aattttcaaa attcatccac aatatatagt gtttgctaga gttggttagc 1610 198 503 PRT NICOTIANATABACUM 198 Met Glu Ile Gln Phe Ser Asn Leu Val Ala Phe Leu Leu Phe Leu Ser 1 5 10 15 Ser Ile Phe Leu Val Phe Lys Lys Trp Lys Thr Arg Lys Leu Asn Leu 20 25 30 Pro Pro Gly Pro Trp Lys Leu Pro Phe Ile Gly Ser Leu His His Leu 35 40 45 Ala Val Ala Gly Pro Leu Pro His His Gly Leu Lys Asn Leu Ala Lys 50 55 60 Arg Tyr Gly Pro Leu Met His Leu Gln Leu Gly Gln Ile Pro Thr Leu 65 70 75 80 Val Ile Ser Ser Pro Gln Met Ala Lys Glu Val Leu Lys Thr His Asp 85 90 95 Leu Ala Phe Ala Thr Arg Pro Lys Leu Val Val Ala Asp Ile Ile His 100 105 110 Tyr Asp Ser Thr Asp Ile Ala Leu Ser Pro Tyr Gly Glu Tyr Trp Arg 115 120 125 Gln Ile Arg Lys Ile Cys Ile Leu Glu Leu Leu Ser Ala Lys Met Val 130 135 140 Lys Phe Phe Ser Ser Ile Arg Gln Asp Glu Leu Ser Lys Met Val Ser 145 150 155 160 Ser Ile Arg Thr Thr Pro Asn Leu Pro Val Asn Leu Thr Asp Lys Ile 165 170 175 Phe Trp Phe Thr Ser Ser Val Ile Cys Arg Ser Ala Leu Gly Lys Ile 180 185 190 Cys Gly Asp Gln Asp Lys Leu Ile Ile Phe Met Arg Glu Ile Ile Ser 195 200 205 Leu Ala Gly Gly Phe Ser Ile Ala Asp Phe Phe Pro Thr Trp Lys Met 210 215 220 Ile His Asp Ile Asp Gly Ser Lys Ser Lys Leu Val Lys Ala His Arg 225 230 235 240 Lys Ile Asp Glu Ile Leu Glu Asn Val Val Asn Glu His Lys Gln Asn 245 250 255 Arg Ala Asp Gly Lys Lys Gly Asn Gly Glu Phe Gly Gly Glu Asp Leu 260 265 270 Ile Asp Val Leu Leu Arg Val Arg Glu Ser Gly Glu Val Gln Ile Pro 275 280 285 Ile Thr Asp Asp Asn Ile Lys Ser Ile Leu Ile Asp Met Phe Ser Ala 290 295 300 Gly Ser Glu Thr Ser Ser Thr Thr Ile Ile Trp Ala Leu Ala Glu Met 305 310 315 320 Met Lys Lys Pro Ser Val Leu Ala Lys Ala Gln Ala Glu Val Ser Gln 325 330 335 Ala Leu Lys Gly Lys Lys Ile Ser Phe Gln Glu Ile Asp Ile Asp Lys 340 345 350 Leu Lys Tyr Leu Lys Leu Val Ile Lys Glu Thr Leu Arg Met His Pro 355 360 365 Pro Ile Pro Leu Leu Val Pro Arg Glu Cys Met Glu Asp Thr Lys Ile 370 375 380 Asp Gly Tyr Asn Ile Pro Phe Lys Thr Arg Val Ile Val Asn Ala Trp 385 390 395 400 Ala Ile Gly Arg Asp Pro Gln Ser Trp Asp Asp Pro Glu Ser Phe Thr 405 410 415 Pro Glu Arg Phe Glu Asn Asn Ser Ile Asp Phe Leu Gly Asn His His 420 425 430 Gln Phe Ile Pro Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly Met Leu 435 440 445 Phe Gly Leu Ala Asn Val Gly Gln Pro Leu Ala Gln Leu Leu Tyr His 450 455 460 Phe Asp Trp Lys Leu Pro Asn Gly Gln Thr His Gln Asn Phe Asp Met 465 470 475 480 Thr Glu Ser Pro Gly Ile Ser Ala Thr Arg Lys Asp Asp Leu Ile Leu 485 490 495 Ile Ala Thr Pro Ala His Ser 500 199 1664 DNA NICOTIANATABACUM 199 atccttcttc cttcctaggt cctaactaaa aatggagatt cagttttcta acttagttgc 60 attcttgctc tttctctcca gcatctttct tctattcaaa aaatggaaaa ccagaaaact 120 aaatttgcct cctggtccat ggaaattacc ttttattgga agtttacacc atttggctgt 180 ggcaggtcca cttcctcacc atggcctaaa aaatttagcc aaacgctatg gtcctcttat 240 gcatttacaa cttggacaaa ttcctacact catcatatca tcacctcaaa tggcaaaaga 300 agtactaaaa actcacgacc tcgcttttgc cactagacca aagcttgtcg tggccgacat 360 cattcactac gacagcacgg acatagcatt ttctccgtac ggtgaatact ggagacaaat 420 tcgtaaaatt tgcatattgg aactcttgag tgccaagatg gtcaaatttt ttagctcgat 480 tcgccaagat gagctctcga agatgctctc atctatacga acgacaccca atcttacagt 540 caatcttact gacaaaattt tttggtttac gagttcggta acttgtagat cagctttagg 600 gaagatatgt ggtgaccaag acaaattgat catttttatg agggaaataa tatcattggc 660 aggtggattt agtattgctg attttttccc tacatggaaa atgattcatg atattgatgg 720 ttcgaaatct aaactggtga aagcacatcg taagattgat gaaattttgg gaaatgttgt 780 tgatgagcac aaaaagaaca gagcagatgg caagaagggt aatggtgaat ttggtggtga 840 agatttgatt gatgtattgt taagagttag agaaagtgga gaagttcaaa ttcctatcac 900 aaatgacaat atcaaatcaa tattaatcga catgttctct gcgggatctg aaacatcatc 960 gacgactata atttgggcat tagctgaaat gatgaagaaa ccaagtgttt tagcaaaggc 1020 acaagctgaa gtaaggcaag ctttgaagga gaaaaaaggt tttcaacaga ttgatcttga 1080 tgagctaaaa tatctcaagt tagtaatcaa agaaacctta agaatgcacc ctccaattcc 1140 tctattagtt cctagagaat gtatggagga tacaaagatt gatggttaca atataccttt 1200 caaaacaaga gtcatagtta atgcatgggc aatcggacga gatccagaaa gttgggatga 1260 ccccgaaagc tttatgccag agagatttga gaatagttct attgactttc ttggaaatca 1320 tcatcagttt ataccatttg gtgcaggaag aaggatttgt ccgggaatgc tatttggttt 1380 agctaatgtt ggacaacctt tagctcagtt actttatcac ttcgattgga aactccctaa 1440 tggacaaagt catgagaatt tcgacatgac tgagtcacct ggaatttctg ctacaagaaa 1500 ggatgatctt gttttgattg ccactcctta tgattcttat taagcagtag cagaaataaa 1560 aagccggggc aaacagaaaa aagtattgct gcttctaggt attttctatt ggataaattt 1620 caaaattcat ccacaatatt tagtgtttgc tagagttggt tagc 1664 200 503 PRT NICOTIANATABACUM 200 Met Glu Ile Gln Phe Ser Asn Leu Val Ala Phe Leu Leu Phe Leu Ser 1 5 10 15 Ser Ile Phe Leu Leu Phe Lys Lys Trp Lys Thr Arg Lys Leu Asn Leu 20 25 30 Pro Pro Gly Pro Trp Lys Leu Pro Phe Ile Gly Ser Leu His His Leu 35 40 45 Ala Val Ala Gly Pro Leu Pro His His Gly Leu Lys Asn Leu Ala Lys 50 55 60 Arg Tyr Gly Pro Leu Met His Leu Gln Leu Gly Gln Ile Pro Thr Leu 65 70 75 80 Ile Ile Ser Ser Pro Gln Met Ala Lys Glu Val Leu Lys Thr His Asp 85 90 95 Leu Ala Phe Ala Thr Arg Pro Lys Leu Val Val Ala Asp Ile Ile His 100 105 110 Tyr Asp Ser Thr Asp Ile Ala Phe Ser Pro Tyr Gly Glu Tyr Trp Arg 115 120 125 Gln Ile Arg Lys Ile Cys Ile Leu Glu Leu Leu Ser Ala Lys Met Val 130 135 140 Lys Phe Phe Ser Ser Ile Arg Gln Asp Glu Leu Ser Lys Met Leu Ser 145 150 155 160 Ser Ile Arg Thr Thr Pro Asn Leu Thr Val Asn Leu Thr Asp Lys Ile 165 170 175 Phe Trp Phe Thr Ser Ser Val Thr Cys Arg Ser Ala Leu Gly Lys Ile 180 185 190 Cys Gly Asp Gln Asp Lys Leu Ile Ile Phe Met Arg Glu Ile Ile Ser 195 200 205 Leu Ala Gly Gly Phe Ser Ile Ala Asp Phe Phe Pro Thr Trp Lys Met 210 215 220 Ile His Asp Ile Asp Gly Ser Lys Ser Lys Leu Val Lys Ala His Arg 225 230 235 240 Lys Ile Asp Glu Ile Leu Gly Asn Val Val Asp Glu His Lys Lys Asn 245 250 255 Arg Ala Asp Gly Lys Lys Gly Asn Gly Glu Phe Gly Gly Glu Asp Leu 260 265 270 Ile Asp Val Leu Leu Arg Val Arg Glu Ser Gly Glu Val Gln Ile Pro 275 280 285 Ile Thr Asn Asp Asn Ile Lys Ser Ile Leu Ile Asp Met Phe Ser Ala 290 295 300 Gly Ser Glu Thr Ser Ser Thr Thr Ile Ile Trp Ala Leu Ala Glu Met 305 310 315 320 Met Lys Lys Pro Ser Val Leu Ala Lys Ala Gln Ala Glu Val Arg Gln 325 330 335 Ala Leu Lys Glu Lys Lys Gly Phe Gln Gln Ile Asp Leu Asp Glu Leu 340 345 350 Lys Tyr Leu Lys Leu Val Ile Lys Glu Thr Leu Arg Met His Pro Pro 355 360 365 Ile Pro Leu Leu Val Pro Arg Glu Cys Met Glu Asp Thr Lys Ile Asp 370 375 380 Gly Tyr Asn Ile Pro Phe Lys Thr Arg Val Ile Val Asn Ala Trp Ala 385 390 395 400 Ile Gly Arg Asp Pro Glu Ser Trp Asp Asp Pro Glu Ser Phe Met Pro 405 410 415 Glu Arg Phe Glu Asn Ser Ser Ile Asp Phe Leu Gly Asn His His Gln 420 425 430 Phe Ile Pro Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly Met Leu Phe 435 440 445 Gly Leu Ala Asn Val Gly Gln Pro Leu Ala Gln Leu Leu Tyr His Phe 450 455 460 Asp Trp Lys Leu Pro Asn Gly Gln Ser His Glu Asn Phe Asp Met Thr 465 470 475 480 Glu Ser Pro Gly Ile Ser Ala Thr Arg Lys Asp Asp Leu Val Leu Ile 485 490 495 Ala Thr Pro Tyr Asp Ser Tyr 500 201 1625 DNA NICOTIANATABACUM 201 ttcttatttt gattcaacca tggagaacca atactcctac tcattctctt cctacttcta 60 cttagctata gtactgtttc ttcttccaat tttggtcaaa tatttcttcc atcggagaag 120 aaatttacct ccaagtccat tttctcttcc aataattggt cacctttacc ttctcaagaa 180 aactctccat ctcactctaa catccttatc agctaaatat ggtcctgttt tatacctcaa 240 attgggctct atgcctgtga ttgttgtgtc ctcaccatct gctgttgaag aatgtttaac 300 caagaatgat atcatattcg caaataggcc caagaccgtg gctggtgaca agtttaccta 360 caattatact gtttatgttt gggcacccta tggccaactt tggagaattc ttcgccgatt 420 aactgtcgtt gaactcttct cttcacatag cctacagaaa acttctatcc ttagagatca 480 agaagttgca atatttatcc gttcgttata caaattctca aaggatagta gcaaaaaagt 540 cgatttgacc aactggtctt ttactttggt tttcaatctt atgaccaaaa ttattgctgg 600 gagacatatt gtgaaggagg aagatgctgg caaggaaaag ggcattgaaa ttattgaaaa 660 acttagaggg actttcttag taactacatc attcttgaat atgtgtgatt tcttgccagt 720 attcaggtgg gttggttaca aagggctgga gaagaagatg gcctcaattc acaatagaag 780 aaatgaattc ttgaacagct tgcttgatga atttcgacac aagaaaagta gtgcttcaca 840 atctaacaca actgttggaa acatggagaa gaaaaccaca ctgattgaaa agctcttgtc 900 tcttcaagaa tcagagcctg aattctacac tgatgatatc atcaaaagta ttatgctggt 960 agtttttgtt gcaggaacag agacctcatc aacaaccatc caatgggtaa tgaggcttct 1020 tgtagctcac cctgaggcat tgtataagct acgagctgac attgacagta aagttgggaa 1080 taagcgcttg ctgaatgaat cagacctcaa caagcttccg tatttgcatt gtgttgttaa 1140 tgagacaatg agattataca ctccgatacc acttttattg cctcattatt caactaaaga 1200 ttgtattgtg gaaggatatg atgtaccaaa acatacaatg ttgtttgtca acgcttgggc 1260 cattcacagg gatcccaagg tatgggagga gcctgacaag ttcaagccag agagatttga 1320 ggcaacagaa ggggaaacag aaaggttcaa ttacaagctt gtaccatttg gaatggggag 1380 aagagcgtgc cctggagctg atatggggtt gcgagcagtt tctttggcat taggtgcact 1440 tattcaatgc tttgactggc aaattgagga agcggaaagc ttggaggaaa gctataattc 1500 tagaatgact atgcagaaca agcctttgaa ggttgtctgc actccacgcg aagatcttgg 1560 ccagcttcta tcccaactct aaggcaattt atcaatgcca aacgtaatct tcatctacca 1620 ctatg 1625 202 520 PRT NICOTIANATABACUM 202 Met Glu Asn Gln Tyr Ser Tyr Ser Phe Ser Ser Tyr Phe Tyr Leu Ala 1 5 10 15 Ile Val Leu Phe Leu Leu Pro Ile Leu Val Lys Tyr Phe Phe His Arg 20 25 30 Arg Arg Asn Leu Pro Pro Ser Pro Phe Ser Leu Pro Ile Ile Gly His 35 40 45 Leu Tyr Leu Leu Lys Lys Thr Leu His Leu Thr Leu Thr Ser Leu Ser 50 55 60 Ala Lys Tyr Gly Pro Val Leu Tyr Leu Lys Leu Gly Ser Met Pro Val 65 70 75 80 Ile Val Val Ser Ser Pro Ser Ala Val Glu Glu Cys Leu Thr Lys Asn 85 90 95 Asp Ile Ile Phe Ala Asn Arg Pro Lys Thr Val Ala Gly Asp Lys Phe 100 105 110 Thr Tyr Asn Tyr Thr Val Tyr Val Trp Ala Pro Tyr Gly Gln Leu Trp 115 120 125 Arg Ile Leu Arg Arg Leu Thr Val Val Glu Leu Phe Ser Ser His Ser 130 135 140 Leu Gln Lys Thr Ser Ile Leu Arg Asp Gln Glu Val Ala Ile Phe Ile 145 150 155 160 Arg Ser Leu Tyr Lys Phe Ser Lys Asp Ser Ser Lys Lys Val Asp Leu 165 170 175 Thr Asn Trp Ser Phe Thr Leu Val Phe Asn Leu Met Thr Lys Ile Ile 180 185 190 Ala Gly Arg His Ile Val Lys Glu Glu Asp Ala Gly Lys Glu Lys Gly 195 200 205 Ile Glu Ile Ile Glu Lys Leu Arg Gly Thr Phe Leu Val Thr Thr Ser 210 215 220 Phe Leu Asn Met Cys Asp Phe Leu Pro Val Phe Arg Trp Val Gly Tyr 225 230 235 240 Lys Gly Leu Glu Lys Lys Met Ala Ser Ile His Asn Arg Arg Asn Glu 245 250 255 Phe Leu Asn Ser Leu Leu Asp Glu Phe Arg His Lys Lys Ser Ser Ala 260 265 270 Ser Gln Ser Asn Thr Thr Val Gly Asn Met Glu Lys Lys Thr Thr Leu 275 280 285 Ile Glu Lys Leu Leu Ser Leu Gln Glu Ser Glu Pro Glu Phe Tyr Thr 290 295 300 Asp Asp Ile Ile Lys Ser Ile Met Leu Val Val Phe Val Ala Gly Thr 305 310 315 320 Glu Thr Ser Ser Thr Thr Ile Gln Trp Val Met Arg Leu Leu Val Ala 325 330 335 His Pro Glu Ala Leu Tyr Lys Leu Arg Ala Asp Ile Asp Ser Lys Val 340 345 350 Gly Asn Lys Arg Leu Leu Asn Glu Ser Asp Leu Asn Lys Leu Pro Tyr 355 360 365 Leu His Cys Val Val Asn Glu Thr Met Arg Leu Tyr Thr Pro Ile Pro 370 375 380 Leu Leu Leu Pro His Tyr Ser Thr Lys Asp Cys Ile Val Glu Gly Tyr 385 390 395 400 Asp Val Pro Lys His Thr Met Leu Phe Val Asn Ala Trp Ala Ile His 405 410 415 Arg Asp Pro Lys Val Trp Glu Glu Pro Asp Lys Phe Lys Pro Glu Arg 420 425 430 Phe Glu Ala Thr Glu Gly Glu Thr Glu Arg Phe Asn Tyr Lys Leu Val 435 440 445 Pro Phe Gly Met Gly Arg Arg Ala Cys Pro Gly Ala Asp Met Gly Leu 450 455 460 Arg Ala Val Ser Leu Ala Leu Gly Ala Leu Ile Gln Cys Phe Asp Trp 465 470 475 480 Gln Ile Glu Glu Ala Glu Ser Leu Glu Glu Ser Tyr Asn Ser Arg Met 485 490 495 Thr Met Gln Asn Lys Pro Leu Lys Val Val Cys Thr Pro Arg Glu Asp 500 505 510 Leu Gly Gln Leu Leu Ser Gln Leu 515 520 203 1767 DNA NICOTIANATABACUM 203 aaccaacctt ccttttctta cttagtaaaa tggatattca gtcttctcct ttcaacttaa 60 ttgctttgct actcttcatt tcatttcttt ttatcctatt gaaaaagtgg aataccaaaa 120 tcccaaagtt acctccaggt ccatggagac ttccccttat tggcagcctc catcacttga 180 aaggtaaact cccacaccat catcttagag atttagcccg aaaatatgga cctctcatgt 240 atttacaact tggagaagtt cctgtagttg taatatcttc gccacgtata gcaaaagctg 300 tactaaaaac tcatgatctt gcttttgcaa cgaggcctcg gttcatgtcc tcggacattg 360 tgttttacaa aagcagggac atatcattcg ccccatatgg cgattactgg agacaaatgc 420 gtaaaatatt aacacaagaa ctcttgagta acaagatgct caagtcattt agcacaatcc 480 gaaaggatga gctctcgaag ctcctctcgt cgattcgttt agcaacagct tcttctgcag 540 tgaacataaa cgaaaagctt ctctggttta caagttgcat gacttgtaga ttagcctttg 600 gaaaaatatg caacgatcgt gatgaattga ttatgttaat aagggagata ttagcattat 660 caggaggatt tgatgtgtgt gatttgttcc cttcatggaa attacttcac aatatgagca 720 acatgaaagc tagattgacg aatgttcacc ataagtataa tctaattatg gagaatatca 780 tcaatgagca caaagagaat catgcagcag ggataaaggg aaataacgag tttggtggcg 840 aagatatgat tgatgcttta ctgagggtta aggagaataa tgagcttcaa tttcctatcg 900 aaaatgacaa catgaaagca gtaattctgg acttgtttat tgctggaact gaaacttcat 960 atactgcaat tatatgggca ctatcagaat tgatgaagca cccaagtgtt atggccaagg 1020 cacaagctga agtgagaaaa gtcttcaaag aaaatgaaaa cttggacgaa aatgatcttg 1080 acaagttgcc atacttaaaa tcagtgatca aagaaacact aaggatgcat cctccagttc 1140 ctttattagg acctagagaa tgcagagaac aaactgagat tgatggatat actgtacctc 1200 ttaaagctag agtaatggtt aatgcatggg caattggaag agatcctgaa agttgggaag 1260 atcctgaaag tttcaaaccc gagcgatttg aaaatatttc tgttgatctt acgggaaatc 1320 actatcagtt catccctttc ggttcaggaa gaagaatgtg tccaggaatg tcgtttggtt 1380 tagttaacac tgggcatcct ttagctcagt tgctctattt ctttgactgg aaattccctc 1440 ataaggttaa tgcagctgat tttcacacta ctgaaacaag tagagttttt gcagcaagca 1500 aagatgacct ctacttgatt ccaacaaatc acatggagca agagtagctc taaattgaat 1560 tcttgtcttg gaacaataaa agaagaaact ccagcttggt ctacattatt tctttttgct 1620 ttatattagt atgggtgtgt tcagtttctt atttttaagg gtaccctgaa agataaaggg 1680 ctatataaac cagtgagact ttttattggt tgcaaggttt tagatcaagc cataagacag 1740 catattttat tcaaaaaaaa aaaaaaa 1767 204 505 PRT NICOTIANATABACUM 204 Met Asp Ile Gln Ser Ser Pro Phe Asn Leu Ile Ala Leu Leu Leu Phe 1 5 10 15 Ile Ser Phe Leu Phe Ile Leu Leu Lys Lys Trp Asn Thr Lys Ile Pro 20 25 30 Lys Leu Pro Pro Gly Pro Trp Arg Leu Pro Leu Ile Gly Ser Leu His 35 40 45 His Leu Lys Gly Lys Leu Pro His His His Leu Arg Asp Leu Ala Arg 50 55 60 Lys Tyr Gly Pro Leu Met Tyr Leu Gln Leu Gly Glu Val Pro Val Val 65 70 75 80 Val Ile Ser Ser Pro Arg Ile Ala Lys Ala Val Leu Lys Thr His Asp 85 90 95 Leu Ala Phe Ala Thr Arg Pro Arg Phe Met Ser Ser Asp Ile Val Phe 100 105 110 Tyr Lys Ser Arg Asp Ile Ser Phe Ala Pro Tyr Gly Asp Tyr Trp Arg 115 120 125 Gln Met Arg Lys Ile Leu Thr Gln Glu Leu Leu Ser Asn Lys Met Leu 130 135 140 Lys Ser Phe Ser Thr Ile Arg Lys Asp Glu Leu Ser Lys Leu Leu Ser 145 150 155 160 Ser Ile Arg Leu Ala Thr Ala Ser Ser Ala Val Asn Ile Asn Glu Lys 165 170 175 Leu Leu Trp Phe Thr Ser Cys Met Thr Cys Arg Leu Ala Phe Gly Lys 180 185 190 Ile Cys Asn Asp Arg Asp Glu Leu Ile Met Leu Ile Arg Glu Ile Leu 195 200 205 Ala Leu Ser Gly Gly Phe Asp Val Cys Asp Leu Phe Pro Ser Trp Lys 210 215 220 Leu Leu His Asn Met Ser Asn Met Lys Ala Arg Leu Thr Asn Val His 225 230 235 240 His Lys Tyr Asn Leu Ile Met Glu Asn Ile Ile Asn Glu His Lys Glu 245 250 255 Asn His Ala Ala Gly Ile Lys Gly Asn Asn Glu Phe Gly Gly Glu Asp 260 265 270 Met Ile Asp Ala Leu Leu Arg Val Lys Glu Asn Asn Glu Leu Gln Phe 275 280 285 Pro Ile Glu Asn Asp Asn Met Lys Ala Val Ile Leu Asp Leu Phe Ile 290 295 300 Ala Gly Thr Glu Thr Ser Tyr Thr Ala Ile Ile Trp Ala Leu Ser Glu 305 310 315 320 Leu Met Lys His Pro Ser Val Met Ala Lys Ala Gln Ala Glu Val Arg 325 330 335 Lys Val Phe Lys Glu Asn Glu Asn Leu Asp Glu Asn Asp Leu Asp Lys 340 345 350 Leu Pro Tyr Leu Lys Ser Val Ile Lys Glu Thr Leu Arg Met His Pro 355 360 365 Pro Val Pro Leu Leu Gly Pro Arg Glu Cys Arg Glu Gln Thr Glu Ile 370 375 380 Asp Gly Tyr Thr Val Pro Leu Lys Ala Arg Val Met Val Asn Ala Trp 385 390 395 400 Ala Ile Gly Arg Asp Pro Glu Ser Trp Glu Asp Pro Glu Ser Phe Lys 405 410 415 Pro Glu Arg Phe Glu Asn Ile Ser Val Asp Leu Thr Gly Asn His Tyr 420 425 430 Gln Phe Ile Pro Phe Gly Ser Gly Arg Arg Met Cys Pro Gly Met Ser 435 440 445 Phe Gly Leu Val Asn Thr Gly His Pro Leu Ala Gln Leu Leu Tyr Phe 450 455 460 Phe Asp Trp Lys Phe Pro His Lys Val Asn Ala Ala Asp Phe His Thr 465 470 475 480 Thr Glu Thr Ser Arg Val Phe Ala Ala Ser Lys Asp Asp Leu Tyr Leu 485 490 495 Ile Pro Thr Asn His Met Glu Gln Glu 500 505 205 1736 DNA NICOTIANATABACUM 205 aaccaacctt ccttttctta cttagtaaaa tggatattca gtcttctcct ttcaacttaa 60 ttgctttgct actcttcatt tcatttcttt ttatcctatt gaaaaagtgg aataccaaaa 120 tcccaaagtt acctccaggt ccatggagac ttccccttat tggcagcctc catcacttga 180 aaggtaaact cccacaccat catcttagag atttagcccg aaaatatgga cctctcatgt 240 atttacaact tggagaagtt cctgtagttg taatatcttc gccacgtata gcaaaagctg 300 tactaaaaac tcatgatctt gcttttgcaa cgaggcctcg gttcatgtcc tcggacattg 360 tgttttacaa aagcagggac atatcattcg ccccatatgg cgattactgg agacaaatgc 420 gtaaaatatt aacacaagaa ctcttgagta acaagatgct caagtcattt agcacaatcc 480 gaaaggatgg gctctcgaag ctcctctcgt cgattcgttt agcaacagct tcttctgcag 540 tgaacataaa cgaaaagctt ctctggttta caagttgcat gacttgtaga ttagcctttg 600 gaaaaatatg caacgatcgt gatgaattga ttatgttaat aagggagata ttagcattat 660 caggaggatt tgatgtgtgt gatttgttcc cttcatggaa attacttcac aatatgagca 720 acatgaaagc tagattgacg aatgttcacc ataagtataa tctaattatg gagaatatca 780 tcaatgagca caaagagaat catgcagcag ggataaaggg aaataacgag tttggtggcg 840 aagatatgat tgatgcttta ctgagggtta aggagaataa tgagcttcaa tttcctatcg 900 aaaatgacaa catgaaagca gtaattctgg acttgtttat tgctggaact gaaacttcat 960 atactgcaat tatatgggca ctatcagaat tgatgaagca cccaagtgtt atggccaagg 1020 cacaagctga agtgagaaaa gtcttcaaag aaaatgaaaa cttggacgaa aatgatcttg 1080 acaagttgcc atacttaaaa tcagtgatca aagaaacact aaggatgcat cctccagttc 1140 ctttattagg acctagagaa tgcagagaac aaactgagat tgatggatat actgtacctc 1200 ttaaagctag agtaatggtt aatgcatggg caattggaag agatcctgaa agttgggaag 1260 atcctgaaag tttcaaaccc gagcgatttg aaaatatttc tgttgatctt acgggaaatc 1320 actatcagtt cattcctttc ggttcaggaa gaagaatgtg tccaggaatg tcgtttggtt 1380 tagttaacac tgggcatcct ttagctcagt tgctctattt ctttgactgg aaattccctc 1440 ataaggttaa tgcagctgat tttcacacta ctgaaacaag tagagttttt gcagcaagca 1500 aagatgacct ctacttgatt ccaacaaatc acatggagca agagtagctc taaattgaat 1560 tcttgtcttg gaacgataaa agaagaaact ccagcttggt ctacattatt tctttttgct 1620 ttatattagt atgggtgtgt tcagtttctt gtttttaagg gtaccctgaa agataaaggg 1680 ctatataaac cagtgagact ttttattgaa aaaaaaaaaa aaaaaaaaaa aaaaaa 1736 206 505 PRT NICOTIANATABACUM 206 Met Asp Ile Gln Ser Ser Pro Phe Asn Leu Ile Ala Leu Leu Leu Phe 1 5 10 15 Ile Ser Phe Leu Phe Ile Leu Leu Lys Lys Trp Asn Thr Lys Ile Pro 20 25 30 Lys Leu Pro Pro Gly Pro Trp Arg Leu Pro Leu Ile Gly Ser Leu His 35 40 45 His Leu Lys Gly Lys Leu Pro His His His Leu Arg Asp Leu Ala Arg 50 55 60 Lys Tyr Gly Pro Leu Met Tyr Leu Gln Leu Gly Glu Val Pro Val Val 65 70 75 80 Val Ile Ser Ser Pro Arg Ile Ala Lys Ala Val Leu Lys Thr His Asp 85 90 95 Leu Ala Phe Ala Thr Arg Pro Arg Phe Met Ser Ser Asp Ile Val Phe 100 105 110 Tyr Lys Ser Arg Asp Ile Ser Phe Ala Pro Tyr Gly Asp Tyr Trp Arg 115 120 125 Gln Met Arg Lys Ile Leu Thr Gln Glu Leu Leu Ser Asn Lys Met Leu 130 135 140 Lys Ser Phe Ser Thr Ile Arg Lys Asp Glu Leu Ser Lys Leu Leu Ser 145 150 155 160 Ser Ile Arg Leu Ala Thr Ala Ser Ser Ala Val Asn Ile Asn Glu Lys 165 170 175 Leu Leu Trp Phe Thr Ser Cys Met Thr Cys Arg Leu Ala Phe Gly Lys 180 185 190 Ile Cys Asn Asp Arg Asp Glu Leu Ile Met Leu Ile Arg Glu Ile Leu 195 200 205 Ala Leu Ser Gly Gly Phe Asp Val Cys Asp Leu Phe Pro Ser Trp Lys 210 215 220 Leu Leu His Asn Met Ser Asn Met Lys Ala Arg Leu Thr Asn Val His 225 230 235 240 His Lys Tyr Asn Leu Ile Met Glu Asn Ile Ile Asn Glu His Lys Glu 245 250 255 Asn His Ala Ala Gly Ile Lys Gly Asn Asn Glu Phe Gly Gly Glu Asp 260 265 270 Met Ile Asp Ala Leu Leu Arg Val Lys Glu Asn Asn Glu Leu Gln Phe 275 280 285 Pro Ile Glu Asn Asp Asn Met Lys Ala Val Ile Leu Asp Leu Phe Ile 290 295 300 Ala Gly Thr Glu Thr Ser Tyr Thr Ala Ile Ile Trp Ala Leu Ser Glu 305 310 315 320 Leu Met Lys His Pro Ser Val Met Ala Lys Ala Gln Ala Glu Val Arg 325 330 335 Lys Val Phe Lys Glu Asn Glu Asn Leu Asp Glu Asn Asp Leu Asp Lys 340 345 350 Leu Pro Tyr Leu Lys Ser Val Ile Lys Glu Thr Leu Arg Met His Pro 355 360 365 Pro Val Pro Leu Leu Gly Pro Arg Glu Cys Arg Glu Gln Thr Glu Ile 370 375 380 Asp Gly Tyr Thr Val Pro Leu Lys Ala Arg Val Met Val Asn Ala Trp 385 390 395 400 Ala Ile Gly Arg Asp Pro Glu Ser Trp Glu Asp Pro Glu Ser Phe Lys 405 410 415 Pro Glu Arg Phe Glu Asn Ile Ser Val Asp Leu Thr Gly Asn His Tyr 420 425 430 Gln Phe Ile Pro Phe Gly Ser Gly Arg Arg Met Cys Pro Gly Met Ser 435 440 445 Phe Gly Leu Val Asn Thr Gly His Pro Leu Ala Gln Leu Leu Tyr Leu 450 455 460 Phe Asp Trp Lys Phe Pro His Lys Val Asn Ala Ala Asp Phe His Thr 465 470 475 480 Thr Glu Thr Ser Arg Val Phe Ala Ala Ser Lys Asp Asp Leu Tyr Leu 485 490 495 Ile Pro Thr Asn His Met Glu Gln Glu 500 505 207 1823 DNA NICOTIANATABACUM 207 aaccaacctt ccttttctta cttagtaaaa tggatattca gtcttctcct ttcaacttaa 60 ttgctttgct actcttcatt tcatttcttt ttatcctatt gaaaaagtgg aataccaaaa 120 tcccaaagtt acctccaggt ccatggagac ttccccttat tggcagcctc catcacttga 180 aaggtaaact cccacaccat catcttagag atttagcccg aaaatatgga cctctcatgt 240 atttacaact tggagaagtt cctgtagttg taatatcttc gccacgtata gcaaaagctg 300 tactaaaaac tcatgatctt gcttttgcaa cgaggcctcg gttcatgtcc tcggacattg 360 tgttttacaa aagcagggac atatcattcg ccccatatgg cgattactgg agacaaatgc 420 gtaaaatatt aacacaagaa ctcttgagta acaagatgct caagtcattt agcacaatcc 480 gaaaggatga gctctcgaag ctcctctcgt cgattcgttt agcaacagct tcttctgcag 540 tgaacataaa cgaaaagctt ctctggttta caagttgcat gacttgtaga ttagcctttg 600 gaaaaatatg caacgatcgt gatgaattga ttatgttaat aagggagata ttagcattat 660 caggaggatt tgatgtgtgt gatttgttcc cttcatggaa attacttcac aatatgagca 720 acatgaaagc tagattgacg aatgttcacc ataagtataa tctaattatg gagaatatca 780 tcaatgagca caaagagaat catgcagcag ggataaaggg aaataacgag tttggtggcg 840 aagatatgat tgatgcttta ctgagggtta aggagaataa tgagcttcaa tttcctatcg 900 aaaatgacaa catgaaagca gtaattctgg acttgtttat tgctggaact gaaacttcat 960 atactgcaat tatatgggca ctatcagaat tgatgaagca cccaagtgtt atggccaagg 1020 cacaagctga agtgagaaaa gtcttcaaag aaaatgaaaa cttggacgaa aatgatcttg 1080 acaagttgcc atacttaaaa tcagtgatca aagaaacact aaggatgcat cctccagttc 1140 ctttattagg acctagagaa tgcagagaac aaactgagat tgatggatat actgtacctc 1200 ttaaagctag agtaatggtt aatgcatggg caattggaag agatcctgaa agttgggaag 1260 atcctgaaag tttcaaaccc gagcgatttg aaaatatttc tgttgatctt acgggaaatc 1320 actatcagtt cattcctttc ggttcaggaa gaagaatgtg tccaggaatg tcgtttggtt 1380 tagttaacac tgggcatcct ttagctcagt tgctctatct ctttgactgg aaattccctc 1440 ataaggttaa tgcagctgat tttcacacta ctgaaacaag tagagttttt gcagcaagca 1500 aagatgacct ctacttgatt ccaacaaatc acatggagca agagtagctc taaattgaat 1560 tcttgtcttg gaacaataaa agaagaaact ccagcttggt ctacattatt tccttttgct 1620 ttatattagt atgggtgtgt tcagtctctt gtttttaagg gtaccctgaa agataaaggg 1680 ctatataaac cagtgagact ttttattggt tgcaaggttt tagatcaagc cataagacag 1740 catattttat tccaccattt tctatcatgt ttaataaagt tcctttcgtt tattgttaga 1800 aaaaaaaaaa aaaaaaaaaa aaa 1823 208 505 PRT NICOTIANATABACUM 208 Met Asp Ile Gln Ser Ser Pro Phe Asn Leu Ile Ala Leu Leu Leu Phe 1 5 10 15 Ile Ser Phe Leu Phe Ile Leu Leu Lys Lys Trp Asn Thr Lys Ile Pro 20 25 30 Lys Leu Pro Pro Gly Pro Trp Arg Leu Pro Leu Ile Gly Ser Leu His 35 40 45 His Leu Lys Gly Lys Leu Pro His His His Leu Arg Asp Leu Ala Arg 50 55 60 Lys Tyr Gly Pro Leu Met Tyr Leu Gln Leu Gly Glu Val Pro Val Val 65 70 75 80 Val Ile Ser Ser Pro Arg Ile Ala Lys Ala Val Leu Lys Thr His Asp 85 90 95 Leu Ala Phe Ala Thr Arg Pro Arg Phe Met Ser Ser Asp Ile Val Phe 100 105 110 Tyr Lys Ser Arg Asp Ile Ser Phe Ala Pro Tyr Gly Asp Tyr Trp Arg 115 120 125 Gln Met Arg Lys Ile Leu Thr Gln Glu Leu Leu Ser Asn Lys Met Leu 130 135 140 Lys Ser Phe Ser Thr Ile Arg Lys Asp Glu Leu Ser Lys Leu Leu Ser 145 150 155 160 Ser Ile Arg Leu Ala Thr Ala Ser Ser Ala Val Asn Ile Asn Glu Lys 165 170 175 Leu Leu Trp Phe Thr Ser Cys Met Thr Cys Arg Leu Ala Phe Gly Lys 180 185 190 Ile Cys Asn Asp Arg Asp Glu Leu Ile Met Leu Ile Arg Glu Ile Leu 195 200 205 Ala Leu Ser Gly Gly Phe Asp Val Cys Asp Leu Phe Pro Ser Trp Lys 210 215 220 Leu Leu His Asn Met Ser Asn Met Lys Ala Arg Leu Thr Asn Val His 225 230 235 240 His Lys Tyr Asn Leu Ile Met Glu Asn Ile Ile Asn Glu His Lys Glu 245 250 255 Asn His Ala Ala Gly Ile Lys Gly Asn Asn Glu Phe Gly Gly Glu Asp 260 265 270 Met Ile Asp Ala Leu Leu Arg Val Lys Glu Asn Asn Glu Leu Gln Phe 275 280 285 Pro Ile Glu Asn Asp Asn Met Lys Ala Val Ile Leu Asp Leu Phe Ile 290 295 300 Ala Gly Thr Glu Thr Ser Tyr Thr Ala Ile Ile Trp Ala Leu Ser Glu 305 310 315 320 Leu Met Lys His Pro Ser Val Met Ala Lys Ala Gln Ala Glu Val Arg 325 330 335 Lys Val Phe Lys Glu Asn Glu Asn Leu Asp Glu Asn Asp Leu Asp Lys 340 345 350 Leu Pro Tyr Leu Lys Ser Val Ile Lys Glu Thr Leu Arg Met His Pro 355 360 365 Pro Val Pro Leu Leu Gly Pro Arg Glu Cys Arg Glu Gln Thr Glu Ile 370 375 380 Asp Gly Tyr Thr Val Pro Leu Lys Ala Arg Val Met Val Asn Ala Trp 385 390 395 400 Ala Ile Gly Arg Asp Pro Glu Ser Trp Glu Asp Pro Glu Ser Phe Lys 405 410 415 Pro Glu Arg Phe Glu Asn Ile Ser Val Asp Leu Thr Gly Asn His Tyr 420 425 430 Gln Phe Ile Pro Phe Gly Ser Gly Arg Arg Met Cys Pro Gly Met Ser 435 440 445 Phe Gly Leu Val Asn Thr Gly His Pro Leu Ala Gln Leu Leu Tyr Leu 450 455 460 Phe Asp Trp Lys Phe Pro His Lys Val Asn Ala Ala Asp Phe His Thr 465 470 475 480 Thr Glu Thr Ser Arg Val Phe Ala Ala Ser Lys Asp Asp Leu Tyr Leu 485 490 495 Ile Pro Thr Asn His Met Glu Gln Glu 500 505 209 1673 DNA NICOTIANATABACUM 209 atatgcaact gagatttgaa gaataccaac taaccaaaat gcagttcttc agcctggttt 60 ccattttcct atttctatct ttcctctttt tgttaagggt atggaagaac tccaatagcc 120 aaagcaaaaa gttgccacca ggtccatgga aactaccaat actaggaagt atgcttcata 180 tggttggtgg actaccacac catgtcctta gagatttagc caaaaaatat ggaccactta 240 tgcaccttca attaggtgaa gtttctgcgg ttgtggttac ttctcctgat acggcaaaag 300 aagtattaaa aactcatgac atcgcttttg cgtctaggcc tagccttttg gccccggaga 360 ttgtctgtta caataggtct gatctagcct tttgccccta tggcgactat tggagacaaa 420 tgcgtaaaat atgtgtcttg gaagtgctca gtgccaagaa tgttcggaca tttagctcta 480 ttaggcggaa tgaagttctt cgtctcatta attttatccg gtcatcttct ggtgaaccta 540 ttaatgttac ggaaaggatc tttttgttca caagctccat gacatgtaga tcagcgtttg 600 ggcaagtgtt caaagagcaa gacaaattta tacaactaat taaagaagtg atactcttag 660 caggagggtt tgatgtggct gacatattcc cttcactgaa gtttcttcat gtgctcagtg 720 gaatgaaggg taagattatg aatgcacacc ataaggtaga tgccattgtt gagaatgtca 780 tcaatgagca caagaaaaat cttgcaattg ggaaaactaa tggagcgtta ggaggtgaag 840 atttaattga tgttcttcta agacttatga atgatggagg ccttcaattt cctatcacca 900 acgacaacat caaagctata atttttgaca tgtttgctgc cgggacagag acttcatcgt 960 caacaattgt gtgggctatg gtagaaatgg tgaaaaatcc agccgtattc gcgaaagctc 1020 aagcagaagt aagagaagca tttagaggaa aagaaacttt cgatgaaaat gatgtggagg 1080 agctaaacta cctaaagtta gtaataaaag aaactctaag acttcatcca ccggttccac 1140 ttttgctccc aagagaatgt agggaagaga caaatataaa cggctacact attcctgtaa 1200 agaccaaagt catggttaat gtttgggctt tgggaagaga tccaaaatat tggaatgacg 1260 cagaaacttt tatgccagag agatttgagc agtgctctaa ggattttgtt ggtaataatt 1320 ttgaatatct tccatttggt ggcggaagga ggatttgtcc tgggatttcg tttggcttag 1380 ctaatgctta tttgccattg gctcaattac tatatcactt cgattggaaa ctccctgctg 1440 gaatcgaacc aagcgacttg gacttgactg agttggttgg agtaactgcc gctagaaaaa 1500 gtgaccttta cttggttgcg actccttatc aacctcctca aaagtgattt aatggtttca 1560 agtttttatt tcctagcaaa ccccactatt gtcctatctt tcttttggtg ttttcggttt 1620 tatctactct aatacatgca tcttttacca tataggaatg taccatgttg tcg 1673 210 514 PRT NICOTIANATABACUM 210 Met Gln Leu Arg Phe Glu Glu Tyr Gln Leu Thr Lys Met Gln Phe Phe 1 5 10 15 Ser Leu Val Ser Ile Phe Leu Phe Leu Ser Phe Leu Phe Leu Leu Arg 20 25 30 Val Trp Lys Asn Ser Asn Ser Gln Ser Lys Lys Leu Pro Pro Gly Pro 35 40 45 Trp Lys Leu Pro Ile Leu Gly Ser Met Leu His Met Val Gly Gly Leu 50 55 60 Pro His His Val Leu Arg Asp Leu Ala Lys Lys Tyr Gly Pro Leu Met 65 70 75 80 His Leu Gln Leu Gly Glu Val Ser Ala Val Val Val Thr Ser Pro Asp 85 90 95 Thr Ala Lys Glu Val Leu Lys Thr His Asp Ile Ala Phe Ala Ser Arg 100 105 110 Pro Ser Leu Leu Ala Pro Glu Ile Val Cys Tyr Asn Arg Ser Asp Leu 115 120 125 Ala Phe Cys Pro Tyr Gly Asp Tyr Trp Arg Gln Met Arg Lys Ile Cys 130 135 140 Val Leu Glu Val Leu Ser Ala Lys Asn Val Arg Thr Phe Ser Ser Ile 145 150 155 160 Arg Arg Asn Glu Val Leu Arg Leu Ile Asn Phe Ile Arg Ser Ser Ser 165 170 175 Gly Glu Pro Ile Asn Val Thr Glu Arg Ile Phe Leu Phe Thr Ser Ser 180 185 190 Met Thr Cys Arg Ser Ala Phe Gly Gln Val Phe Lys Glu Gln Asp Lys 195 200 205 Phe Ile Gln Leu Ile Lys Glu Val Ile Leu Leu Ala Gly Gly Phe Asp 210 215 220 Val Ala Asp Ile Phe Pro Ser Leu Lys Phe Leu His Val Leu Ser Gly 225 230 235 240 Met Lys Gly Lys Ile Met Asn Ala His His Lys Val Asp Ala Ile Val 245 250 255 Glu Asn Val Ile Asn Glu His Lys Lys Asn Leu Ala Ile Gly Lys Thr 260 265 270 Asn Gly Ala Leu Gly Gly Glu Asp Leu Ile Asp Val Leu Leu Arg Leu 275 280 285 Met Asn Asp Gly Gly Leu Gln Phe Pro Ile Thr Asn Asp Asn Ile Lys 290 295 300 Ala Ile Ile Phe Asp Met Phe Ala Ala Gly Thr Glu Thr Ser Ser Ser 305 310 315 320 Thr Ile Val Trp Ala Met Val Glu Met Val Lys Asn Pro Ala Val Phe 325 330 335 Ala Lys Ala Gln Ala Glu Val Arg Glu Ala Phe Arg Gly Lys Glu Thr 340 345 350 Phe Asp Glu Asn Asp Val Glu Glu Leu Asn Tyr Leu Lys Leu Val Ile 355 360 365 Lys Glu Thr Leu Arg Leu His Pro Pro Val Pro Leu Leu Leu Pro Arg 370 375 380 Glu Cys Arg Glu Glu Thr Asn Ile Asn Gly Tyr Thr Ile Pro Val Lys 385 390 395 400 Thr Lys Val Met Val Asn Val Trp Ala Leu Gly Arg Asp Pro Lys Tyr 405 410 415 Trp Asn Asp Ala Glu Thr Phe Met Pro Glu Arg Phe Glu Gln Cys Ser 420 425 430 Lys Asp Phe Val Gly Asn Asn Phe Glu Tyr Leu Pro Phe Gly Gly Gly 435 440 445 Arg Arg Ile Cys Pro Gly Ile Ser Phe Gly Leu Ala Asn Ala Tyr Leu 450 455 460 Pro Leu Ala Gln Leu Leu Tyr His Phe Asp Trp Lys Leu Pro Ala Gly 465 470 475 480 Ile Glu Pro Ser Asp Leu Asp Leu Thr Glu Leu Val Gly Val Thr Ala 485 490 495 Ala Arg Lys Ser Asp Leu Tyr Leu Val Ala Thr Pro Tyr Gln Pro Pro 500 505 510 Gln Lys 211 1673 DNA NICOTIANATABACUM 211 atatgcaact gagatttgaa gaataccaac taaccaaaat gcagttcttc agcttggttt 60 ccattttcct atttctatct ttcctctttt tgttaaggat atggaagaac tccaatagcc 120 aaagcaaaaa gttgccacca ggtccatgga aactaccaat actaggaagt atgcttcata 180 tggttggtgg actaccacac catgtcctta gagatttagc caaaaaatat ggaccactta 240 tgcaccttca attaggtgaa gtttctgcgg ttgtggttac ttctcctgat acggcaaaag 300 aagtattaaa aactcatgac atcgcttttg cgtctaggcc tagccttttg gccccggaga 360 ttgtctgtta caataggtct gatctagcct tttgccccta tggcgactat tggagacaaa 420 tgcgtaaaat atgtgtcttg gaagtgctca gtgccaagaa tgttcggaca tttagctcta 480 ttaggcggaa tgaagttctt cgtctcatta attttatccg gtcatcttct ggtgaaccta 540 ttaatgttac ggaaaggatc tttttgttca caagctccat gacatgtaga tcagcgtttg 600 ggcaagtgtt caaagagcaa gacaaattta tacaactaat taaagaagtg atactcttag 660 caggagggtt tgatgtggct gacatattcc cttcactgaa gtttcttcat gtgctcagtg 720 gaatgaaggg taagattatg aatgcacacc ataaggtaga tgccattgtt gagaatgtca 780 tcaatgagca caagaaaaat cttgcaattg ggaaaactaa tggagcgtta ggaggtgaag 840 atttaattga tgttcttcta agacttatga atgatggagg ccttcaattt cctatcacca 900 acgacaacat caaagccata atttttgaca tgtttgctgc cgggacagag acttcatcgt 960 caacaattgt gtgggctatg gtagaaatgg tgaaaaatcc agccgtattc gcgaaagctc 1020 aagcagaagt aagagaagca tttagaggaa aagaaacttt cgatgaaaat gatgtggagg 1080 agctaaacta cctaaagtta gtaataaaag aaactctaag acttcatcca ccggttccac 1140 ttttgctccc aagagaatgt agggaagaga caaatataaa cggctacact attcctgtaa 1200 agaccaaagt catggttaat gtttgggctt tgggaagaga tccaaaatat tggaatgacg 1260 cagaaacttt tatgccagag agatttgagc agtgctctaa ggattttgtt ggtaataatt 1320 ttgaatatct tccatttggt ggcggaagga ggatttgtcc tgggatttcg tttggcttag 1380 ctaatgctta tttgccattg gctcaattac tatatcactt cgattggaaa ctccctgctg 1440 gaatcgaacc aagcgacttg gacttgactg agttggttgg agtaactgcc gctagaaaaa 1500 gtgaccttta cttggttgcg actccttatc aacctcctca aaagtgattt aatggtttca 1560 agtttttatt tcctagcaaa ccccactatt gtcctatctt tcttttggtg ttttcggttt 1620 tatctactct aatacatgca tcttttacca tataggaatg taccatgttg tcg 1673 212 514 PRT NICOTIANATABACUM 212 Met Gln Leu Arg Phe Glu Glu Tyr Gln Leu Thr Lys Met Gln Phe Phe 1 5 10 15 Ser Leu Val Ser Ile Phe Leu Phe Leu Ser Phe Leu Phe Leu Leu Arg 20 25 30 Ile Trp Lys Asn Ser Asn Ser Gln Ser Lys Lys Leu Pro Pro Gly Pro 35 40 45 Trp Lys Leu Pro Ile Leu Gly Ser Met Leu His Met Val Gly Gly Leu 50 55 60 Pro His His Val Leu Arg Asp Leu Ala Lys Lys Tyr Gly Pro Leu Met 65 70 75 80 His Leu Gln Leu Gly Glu Val Ser Ala Val Val Val Thr Ser Pro Asp 85 90 95 Thr Ala Lys Glu Val Leu Lys Thr His Asp Ile Ala Phe Ala Ser Arg 100 105 110 Pro Ser Leu Leu Ala Pro Glu Ile Val Cys Tyr Asn Arg Ser Asp Leu 115 120 125 Ala Phe Cys Pro Tyr Gly Asp Tyr Trp Arg Gln Met Arg Lys Ile Cys 130 135 140 Val Leu Glu Val Leu Ser Ala Lys Asn Val Arg Thr Phe Ser Ser Ile 145 150 155 160 Arg Arg Asn Glu Val Leu Arg Leu Ile Asn Phe Ile Arg Ser Ser Ser 165 170 175 Gly Glu Pro Ile Asn Val Thr Glu Arg Ile Phe Leu Phe Thr Ser Ser 180 185 190 Met Thr Cys Arg Ser Ala Phe Gly Gln Val Phe Lys Glu Gln Asp Lys 195 200 205 Phe Ile Gln Leu Ile Lys Glu Val Ile Leu Leu Ala Gly Gly Phe Asp 210 215 220 Val Ala Asp Ile Phe Pro Ser Leu Lys Phe Leu His Val Leu Ser Gly 225 230 235 240 Met Lys Gly Lys Ile Met Asn Ala His His Lys Val Asp Ala Ile Val 245 250 255 Glu Asn Val Ile Asn Glu His Lys Lys Asn Leu Ala Ile Gly Lys Thr 260 265 270 Asn Gly Ala Leu Gly Gly Glu Asp Leu Ile Asp Val Leu Leu Arg Leu 275 280 285 Met Asn Asp Gly Gly Leu Gln Phe Pro Ile Thr Asn Asp Asn Ile Lys 290 295 300 Ala Ile Ile Phe Asp Met Phe Ala Ala Gly Thr Glu Thr Ser Ser Ser 305 310 315 320 Thr Ile Val Trp Ala Met Val Glu Met Val Lys Asn Pro Ala Val Phe 325 330 335 Ala Lys Ala Gln Ala Glu Val Arg Glu Ala Phe Arg Gly Lys Glu Thr 340 345 350 Phe Asp Glu Asn Asp Val Glu Glu Leu Asn Tyr Leu Lys Leu Val Ile 355 360 365 Lys Glu Thr Leu Arg Leu His Pro Pro Val Pro Leu Leu Leu Pro Arg 370 375 380 Glu Cys Arg Glu Glu Thr Asn Ile Asn Gly Tyr Thr Ile Pro Val Lys 385 390 395 400 Thr Lys Val Met Val Asn Val Trp Ala Leu Gly Arg Asp Pro Lys Tyr 405 410 415 Trp Asn Asp Ala Glu Thr Phe Met Pro Glu Arg Phe Glu Gln Cys Ser 420 425 430 Lys Asp Phe Val Gly Asn Asn Phe Glu Tyr Leu Pro Phe Gly Gly Gly 435 440 445 Arg Arg Ile Cys Pro Gly Ile Ser Phe Gly Leu Ala Asn Ala Tyr Leu 450 455 460 Pro Leu Ala Gln Leu Leu Tyr His Phe Asp Trp Lys Leu Pro Ala Gly 465 470 475 480 Ile Glu Pro Ser Asp Leu Asp Leu Thr Glu Leu Val Gly Val Thr Ala 485 490 495 Ala Arg Lys Ser Asp Leu Tyr Leu Val Ala Thr Pro Tyr Gln Pro Pro 500 505 510 Gln Lys 213 1673 DNA NICOTIANATABACUM 213 atatgcaact gagatttgaa gaataccaac taaccaaagt gcagttcttc agcttggttt 60 ccattttcct atttctatct ttcctctttt tgttaaggat atggaagaac tccaatagcc 120 aaagcaaaaa gttgccacca ggtccatgga aactaccaat actaggaagt atgcttcata 180 tggttggtgg actaccacac catgtcctta gagatttagc caaaaaatat ggaccactta 240 tgcaccttca attaggtgaa gtttctgcgg ttgtggttac ttctcctgat acggcaaaag 300 aagtattaaa aactcatgac atcgcttttg cgtctaggcc tagccttttg gccccggaga 360 ttgtctgtta caataggtct gatctagcct tttgccccta tggcgactat tggagacaaa 420 tgcgtaaaat atgtgtcttg gaagtgctca gtgccaagaa tgttcggaca tttagctcta 480 ttaggcggaa tgaagttctt cgtctcatta attttatccg gtcatcttct ggtgaaccta 540 ttaatgttac ggaaaggatc tttttgttca caagctccat gacatgtaga tcagcgtttg 600 ggcaagtgtt caaagagcaa gacaaattta tacaactaat taaagaagtg atactcttag 660 caggagggtt tgatgtggct gacatattcc cttcactgaa gtttcttcat gtgctcagtg 720 gaatgaaggg taagattatg aatgcacacc ataaggtaga tgccattgtt gagaatgtca 780 tcaatgagca caagaaaaat cttgcaattg ggaaaactaa tggagcgtta ggaggtgaag 840 atttaattga tgttcctcta agacttatga atgatggagg ccttcaattt cctatcacca 900 acgacaacat caaagctata atttttgaca tgtttgctgc cgggacggag acttcatcgt 960 caacaattgt gtgggctatg gtagaaatgg tgaaaaatcc agccgtattc gcgaaagctc 1020 aagcagaagt aagagaagca tttagaggaa aagaaacttt cgatgaaaat gatgtggagg 1080 agctaaacta cctaaagtta gtaataaaag aaactctaag acttcatcca ccggttccac 1140 ttttgctccc aagagaatgt agggaagaga caaatataaa cggctacact attcctgtaa 1200 agaccaaagt catggttaat gtttgggctt tgggaagaga tccaaaatat tggaatgacg 1260 cagaaacttt tatgccagag agatttgagc agtgctctaa ggattttgtt ggtaataatt 1320 ttgaatatct tccatttggt ggcggaagga ggatttgtcc tgggatttcg tttggcttag 1380 ctaatgctta tttgccattg gctcaattac tatatcactt cgattggaaa ctccctgctg 1440 gaatcgaacc aagcgacttg gacttgactg agttggttgg agtaactgcc gctagaaaaa 1500 gtgaccttta cttggttgcg actccttatc aacctcctca aaagtgattt aatggtttca 1560 agtttttatt tcctagcaaa ccccactatt gtcctatctt tcttttggtg ttttcggttt 1620 tatctactct aatacatgca tcttttacca tataggaatg taccatgttg tcg 1673 214 514 PRT NICOTIANATABACUM 214 Met Gln Leu Arg Phe Glu Glu Tyr Gln Leu Thr Lys Val Gln Phe Phe 1 5 10 15 Ser Leu Val Ser Ile Phe Leu Phe Leu Ser Phe Leu Phe Leu Leu Arg 20 25 30 Ile Trp Lys Asn Ser Asn Ser Gln Ser Lys Lys Leu Pro Pro Gly Pro 35 40 45 Trp Lys Leu Pro Ile Leu Gly Ser Met Leu His Met Val Gly Gly Leu 50 55 60 Pro His His Val Leu Arg Asp Leu Ala Lys Lys Tyr Gly Pro Leu Met 65 70 75 80 His Leu Gln Leu Gly Glu Val Ser Ala Val Val Val Thr Ser Pro Asp 85 90 95 Thr Ala Lys Glu Val Leu Lys Thr His Asp Ile Ala Phe Ala Ser Arg 100 105 110 Pro Ser Leu Leu Ala Pro Glu Ile Val Cys Tyr Asn Arg Ser Asp Leu 115 120 125 Ala Phe Cys Pro Tyr Gly Asp Tyr Trp Arg Gln Met Arg Lys Ile Cys 130 135 140 Val Leu Glu Val Leu Ser Ala Lys Asn Val Arg Thr Phe Ser Ser Ile 145 150 155 160 Arg Arg Asn Glu Val Leu Arg Leu Ile Asn Phe Ile Arg Ser Ser Ser 165 170 175 Gly Glu Pro Ile Asn Val Thr Glu Arg Ile Phe Leu Phe Thr Ser Ser 180 185 190 Met Thr Cys Arg Ser Ala Phe Gly Gln Val Phe Lys Glu Gln Asp Lys 195 200 205 Phe Ile Gln Leu Ile Lys Glu Val Ile Leu Leu Ala Gly Gly Phe Asp 210 215 220 Val Ala Asp Ile Phe Pro Ser Leu Lys Phe Leu His Val Leu Ser Gly 225 230 235 240 Met Lys Gly Lys Ile Met Asn Ala His His Lys Val Asp Ala Ile Val 245 250 255 Glu Asn Val Ile Asn Glu His Lys Lys Asn Leu Ala Ile Gly Lys Thr 260 265 270 Asn Gly Ala Leu Gly Gly Glu Asp Leu Ile Asp Val Pro Leu Arg Leu 275 280 285 Met Asn Asp Gly Gly Leu Gln Phe Pro Ile Thr Asn Asp Asn Ile Lys 290 295 300 Ala Ile Ile Phe Asp Met Phe Ala Ala Gly Thr Glu Thr Ser Ser Ser 305 310 315 320 Thr Ile Val Trp Ala Met Val Glu Met Val Lys Asn Pro Ala Val Phe 325 330 335 Ala Lys Ala Gln Ala Glu Val Arg Glu Ala Phe Arg Gly Lys Glu Thr 340 345 350 Phe Asp Glu Asn Asp Val Glu Glu Leu Asn Tyr Leu Lys Leu Val Ile 355 360 365 Lys Glu Thr Leu Arg Leu His Pro Pro Val Pro Leu Leu Leu Pro Arg 370 375 380 Glu Cys Arg Glu Glu Thr Asn Ile Asn Gly Tyr Thr Ile Pro Val Lys 385 390 395 400 Thr Lys Val Met Val Asn Val Trp Ala Leu Gly Arg Asp Pro Lys Tyr 405 410 415 Trp Asn Asp Ala Glu Thr Phe Met Pro Glu Arg Phe Glu Gln Cys Ser 420 425 430 Lys Asp Phe Val Gly Asn Asn Phe Glu Tyr Leu Pro Phe Gly Gly Gly 435 440 445 Arg Arg Ile Cys Pro Gly Ile Ser Phe Gly Leu Ala Asn Ala Tyr Leu 450 455 460 Pro Leu Ala Gln Leu Leu Tyr His Phe Asp Trp Lys Leu Pro Ala Gly 465 470 475 480 Ile Glu Pro Ser Asp Leu Asp Leu Thr Glu Leu Val Gly Val Thr Ala 485 490 495 Ala Arg Lys Ser Asp Leu Tyr Leu Val Ala Thr Pro Tyr Gln Pro Pro 500 505 510 Gln Lys 215 1580 DNA NICOTIANATABACUM 215 gaaatgggaa atgctcacaa cagcaaaatt gcagcaatct gtttgataat tttcttggta 60 tataaagcat gggaattgtt gaagtggata tggattaagc caaagaaact ggagagttgc 120 ctcagaaaac agggactcaa aggaaattcc tacaggctat tctatggaga tatgaaagaa 180 ttgtccaaaa gtctcaagga aatcaattca aagcccatca tcaatctatc aaatgaagta 240 gccccaagaa tcattcctta ttatcttgaa atcatccaaa aatatggtaa aagatgtttt 300 gtttggcaag gaccaacccc cgcaatatta ataacagagc cagaattaat aaaggagata 360 tttggtaaga actatgtttt tcagaagcct aataatccca acccactgac caagttattg 420 gctcgaggtg ttgtaagcta cgaggaagaa aaatgggcaa aacacagaaa gatcttaaac 480 cctgcctttc atatggagaa gttgaagcat atgctaccag cattttactt gagctgtagt 540 gagatgctga acaaatggga ggagattatc ccagtaaaag aatcaaatga gttggacatt 600 tggcctcatc ttcaaagaat gacaagtgat gtgatttctc gtgctgcctt tggtagtagc 660 tacgaagaag gaagaagaat atttgaactt caagaagaac aagctgagta tctaacgaag 720 acattcaatt cagtttatat cccaggttcc agattttttc ccaataaaat gaacaaaaga 780 atgaaagaat gtgaaaagga agtacgagaa acaattacgt gtctaattga caacagatta 840 aaggcaaaag aagaaggcaa tggcaaggcc ctcaatgatg acctactggg tatattatta 900 gagtcaaatt ctatagaaat tgaagaacat ggtaacaaga agtttggaat gagtatacct 960 gaagtaattg aagagtgcaa attattctat tttgctggcc aagagactac atcagtattg 1020 cttgtgtgga cactgatttt gttagggaga aatccagaat ggcaggaacg tgctagagag 1080 gaagtttttc aagcctttgg aagtgataaa ccaacttttg acgaattata tcgcttgaaa 1140 attgtgacga tgattttgta cgagtcttta aggttatatc caccaatagc aactcgtact 1200 cgaaggacta atgaagaaac aaaattaggg gaactagatt taccaaaggg tgcactgctc 1260 tttataccaa caatcttatt acatcttgac aaggaaattt ggggtgaaga tgcagatgag 1320 ttcaatccgg agagatttag cgaaggggtg gcaaaggcaa caaaggggaa aatgacatat 1380 tttccatttg gtgcaggacc gcgaaaatgc attgggcaaa acttcgcgat tttggaagca 1440 aaaatggcta tagctatgat tctacaacgc ttctccttcg agctctctcc atcttataca 1500 cactctccat acactgtggt cactttgaaa cccaaatatg gtgctcccct aataatgcac 1560 aggctgtagt cctgtgagaa 1580 216 521 PRT NICOTIANATABACUM 216 Met Gly Asn Ala His Asn Ser Lys Ile Ala Ala Ile Cys Leu Ile Ile 1 5 10 15 Phe Leu Val Tyr Lys Ala Trp Glu Leu Leu Lys Trp Ile Trp Ile Lys 20 25 30 Pro Lys Lys Leu Glu Ser Cys Leu Arg Lys Gln Gly Leu Lys Gly Asn 35 40 45 Ser Tyr Arg Leu Phe Tyr Gly Asp Met Lys Glu Leu Ser Lys Ser Leu 50 55 60 Lys Glu Ile Asn Ser Lys Pro Ile Ile Asn Leu Ser Asn Glu Val Ala 65 70 75 80 Pro Arg Ile Ile Pro Tyr Tyr Leu Glu Ile Ile Gln Lys Tyr Gly Lys 85 90 95 Arg Cys Phe Val Trp Gln Gly Pro Thr Pro Ala Ile Leu Ile Thr Glu 100 105 110 Pro Glu Leu Ile Lys Glu Ile Phe Gly Lys Asn Tyr Val Phe Gln Lys 115 120 125 Pro Asn Asn Pro Asn Pro Leu Thr Lys Leu Leu Ala Arg Gly Val Val 130 135 140 Ser Tyr Glu Glu Glu Lys Trp Ala Lys His Arg Lys Ile Leu Asn Pro 145 150 155 160 Ala Phe His Met Glu Lys Leu Lys His Met Leu Pro Ala Phe Tyr Leu 165 170 175 Ser Cys Ser Glu Met Leu Asn Lys Trp Glu Glu Ile Ile Pro Val Lys 180 185 190 Glu Ser Asn Glu Leu Asp Ile Trp Pro His Leu Gln Arg Met Thr Ser 195 200 205 Asp Val Ile Ser Arg Ala Ala Phe Gly Ser Ser Tyr Glu Glu Gly Arg 210 215 220 Arg Ile Phe Glu Leu Gln Glu Glu Gln Ala Glu Tyr Leu Thr Lys Thr 225 230 235 240 Phe Asn Ser Val Tyr Ile Pro Gly Ser Arg Phe Phe Pro Asn Lys Met 245 250 255 Asn Lys Arg Met Lys Glu Cys Glu Lys Glu Val Arg Glu Thr Ile Thr 260 265 270 Cys Leu Ile Asp Asn Arg Leu Lys Ala Lys Glu Glu Gly Asn Gly Lys 275 280 285 Ala Leu Asn Asp Asp Leu Leu Gly Ile Leu Leu Glu Ser Asn Ser Ile 290 295 300 Glu Ile Glu Glu His Gly Asn Lys Lys Phe Gly Met Ser Ile Pro Glu 305 310 315 320 Val Ile Glu Glu Cys Lys Leu Phe Tyr Phe Ala Gly Gln Glu Thr Thr 325 330 335 Ser Val Leu Leu Val Trp Thr Leu Ile Leu Leu Gly Arg Asn Pro Glu 340 345 350 Trp Gln Glu Arg Ala Arg Glu Glu Val Phe Gln Ala Phe Gly Ser Asp 355 360 365 Lys Pro Thr Phe Asp Glu Leu Tyr Arg Leu Lys Ile Val Thr Met Ile 370 375 380 Leu Tyr Glu Ser Leu Arg Leu Tyr Pro Pro Ile Ala Thr Arg Thr Arg 385 390 395 400 Arg Thr Asn Glu Glu Thr Lys Leu Gly Glu Leu Asp Leu Pro Lys Gly 405 410 415 Ala Leu Leu Phe Ile Pro Thr Ile Leu Leu His Leu Asp Lys Glu Ile 420 425 430 Trp Gly Glu Asp Ala Asp Glu Phe Asn Pro Glu Arg Phe Ser Glu Gly 435 440 445 Val Ala Lys Ala Thr Lys Gly Lys Met Thr Tyr Phe Pro Phe Gly Ala 450 455 460 Gly Pro Arg Lys Cys Ile Gly Gln Asn Phe Ala Ile Leu Glu Ala Lys 465 470 475 480 Met Ala Ile Ala Met Ile Leu Gln Arg Phe Ser Phe Glu Leu Ser Pro 485 490 495 Ser Tyr Thr His Ser Pro Tyr Thr Val Val Thr Leu Lys Pro Lys Tyr 500 505 510 Gly Ala Pro Leu Ile Met His Arg Leu 515 520 217 1587 DNA NICOTIANATABACUM 217 atgctttctc ccatagaagc ctttgtagga ctagtaacct tcacatttct cttatacttc 60 ctatggacaa aaaaatctca aaaacttcca aaacccttac caccgaaaat ccccggagga 120 tggccggtaa tcggccatct ttttcacttc aataacgacg gcgacgaccg tccattagct 180 cgaaagctcg gagacttagc tgataaatac ggccccgttt tcacttttcg gctaggtctt 240 ccccttgtgc tagttgtaag cagttacgaa gctataaaag attgcttctc tacaaatgat 300 gccattttct ccaatcgtcc agctcttctt tacggcgaat accttggcta caataataca 360 atgctttttc tagcaaatta cggaccttac tggcgaaaaa atcgtaaatt agtcattcag 420 gaagttctct ctgctagtcg tctcgaaaaa ttcaaacaag tgagattcac cagaattcaa 480 acgagcatta agaatttata cactcgaatt aatggaaatt cgagtacgat aaatctaact 540 gattggttag aagaattgaa ttttggtctg atcgtgaaaa tgatcgctgg gaaaaattat 600 gaatccggta aaggagatga acaagtggaa agatttaaga atgcgtttaa ggattttatg 660 gttttatcaa tggaatttgt attatgggat gcatttccaa ttccattatt taaatgggtg 720 gattttcaag gtcatattaa ggcaatgaaa aggacattta aggatataga ttctgttttt 780 cagaactggt tagaggaaca tattaataaa agagaaaaaa tagaggttgg tgcagaaggg 840 aatgaacaag atttcattga tgtggtgctt tcaaaattga gtaaagaata tcttgatgaa 900 ggttactctc gtgatactgt cattaaagca acagttttta gtttggtctt ggatgcagca 960 gacacagttg ctcttcacat aaattgggga atgacattat tgataaacaa tcaaaatgcc 1020 ttgatgaaag cacaagaaga gatagacaca aaagttggta aggatagatg ggtagaagag 1080 agtgatatta aggatttagt atacctccaa gctattgtta aaaaggtgtt acgattatat 1140 ccaccaggac ctttgttagt accacatgaa aatgtaaagg attgtgttgt tagtggatat 1200 cacattccta aagggactag attattcgca aacgtcatga aactgcagcg cgatcctaaa 1260 ctcttgtcaa atcctgataa gttcgatcca gagagattca tcgctggtga tattgacttc 1320 cgtggtcacc actatgagtt tatcccattt ggttctggaa gacgatcttg tccggggatg 1380 acttatgcat tgcaagtgga acacctaaca atggcacatt taatccaggg tttcaattac 1440 aaaactccaa atgacgaggc cttggatatg aaggaaggtg caggcataac aatacgtaag 1500 gtaaatccag tggaattgat aataacgcct cgcttggcac ctgagcttta ctaaaaccta 1560 agatgtttca tcttggttga tcattgt 1587 218 517 PRT NICOTIANATABACUM 218 Met Leu Ser Pro Ile Glu Ala Phe Val Gly Leu Val Thr Phe Thr Phe 1 5 10 15 Leu Leu Tyr Phe Leu Trp Thr Lys Lys Ser Gln Lys Leu Pro Lys Pro 20 25 30 Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe 35 40 45 His Phe Asn Asn Asp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly 50 55 60 Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu 65 70 75 80 Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Ile Lys Asp Cys Phe 85 90 95 Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Leu Leu Tyr Gly 100 105 110 Glu Tyr Leu Gly Tyr Asn Asn Thr Met Leu Phe Leu Ala Asn Tyr Gly 115 120 125 Pro Tyr Trp Arg Lys Asn Arg Lys Leu Val Ile Gln Glu Val Leu Ser 130 135 140 Ala Ser Arg Leu Glu Lys Phe Lys Gln Val Arg Phe Thr Arg Ile Gln 145 150 155 160 Thr Ser Ile Lys Asn Leu Tyr Thr Arg Ile Asn Gly Asn Ser Ser Thr 165 170 175 Ile Asn Leu Thr Asp Trp Leu Glu Glu Leu Asn Phe Gly Leu Ile Val 180 185 190 Lys Met Ile Ala Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu Gln 195 200 205 Val Glu Arg Phe Lys Asn Ala Phe Lys Asp Phe Met Val Leu Ser Met 210 215 220 Glu Phe Val Leu Trp Asp Ala Phe Pro Ile Pro Leu Phe Lys Trp Val 225 230 235 240 Asp Phe Gln Gly His Ile Lys Ala Met Lys Arg Thr Phe Lys Asp Ile 245 250 255 Asp Ser Val Phe Gln Asn Trp Leu Glu Glu His Ile Asn Lys Arg Glu 260 265 270 Lys Ile Glu Val Gly Ala Glu Gly Asn Glu Gln Asp Phe Ile Asp Val 275 280 285 Val Leu Ser Lys Leu Ser Lys Glu Tyr Leu Asp Glu Gly Tyr Ser Arg 290 295 300 Asp Thr Val Ile Lys Ala Thr Val Phe Ser Leu Val Leu Asp Ala Ala 305 310 315 320 Asp Thr Val Ala Leu His Ile Asn Trp Gly Met Thr Leu Leu Ile Asn 325 330 335 Asn Gln Asn Ala Leu Met Lys Ala Gln Glu Glu Ile Asp Thr Lys Val 340 345 350 Gly Lys Asp Arg Trp Val Glu Glu Ser Asp Ile Lys Asp Leu Val Tyr 355 360 365 Leu Gln Ala Ile Val Lys Lys Val Leu Arg Leu Tyr Pro Pro Gly Pro 370 375 380 Leu Leu Val Pro His Glu Asn Val Lys Asp Cys Val Val Ser Gly Tyr 385 390 395 400 His Ile Pro Lys Gly Thr Arg Leu Phe Ala Asn Val Met Lys Leu Gln 405 410 415 Arg Asp Pro Lys Leu Leu Ser Asn Pro Asp Lys Phe Asp Pro Glu Arg 420 425 430 Phe Ile Ala Gly Asp Ile Asp Phe Arg Gly His His Tyr Glu Phe Ile 435 440 445 Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Met Thr Tyr Ala Leu 450 455 460 Gln Val Glu His Leu Thr Met Ala His Leu Ile Gln Gly Phe Asn Tyr 465 470 475 480 Lys Thr Pro Asn Asp Glu Ala Leu Asp Met Lys Glu Gly Ala Gly Ile 485 490 495 Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile Ile Thr Pro Arg Leu 500 505 510 Ala Pro Glu Leu Tyr 515 219 1634 DNA NICOTIANATABACUM 219 gggagaaggc cttcaatatg gagataccat attacagctt aaaaattgca atttcttcat 60 ttgcaattat ctttgtacta agatgggcat ggaaaatctt gaattatgtg tggttaaaac 120 caaaagaatt ggagaaatac ctcagacagc agggtttcaa aggaaactct tacaaattct 180 tgtttgggga tatgaaagag acgaagaaaa tgggtgaaga agctatgtct aagccaatca 240 atttctctca tgacatgatt tggcctagag ttatgccatt catccacaaa accatcacca 300 attatggtaa gaattgtatt gtgtggtttg ggccaagacc agcagtcctg atcacagacc 360 cggaacttgt aaaggaggtg ctaacgaaga atttcgtcta tcagaagccg cttggcaatc 420 cactcacaaa gttggcagca actggaattg caggctatga aacagataaa tgggctacac 480 atagaaggct tctcaatcct gcttttcacc ttgacaagtt gaagcatatg ctacctgcat 540 tccaatttac tgctagtgag atgttgagca aattggagaa agttgtttca ccaaacggaa 600 cagagataga tgtgtggcca tatttacaaa ctttgacaag tgatgccatt tcaagaactg 660 cgtttggaag tagttatgaa gaaggaagaa agatttttga ccttcaaaaa gaacaacttt 720 cactaattct agaagtttca cgcacaatat atattccagg atggaggttt ttgccaacga 780 aaaggaacaa aaggatgaag caaatattta atgaagtacg agcactggta tttggaatta 840 ttaagaaaag gatgagtatg attgaaaatg gagaagcacc tgatgattta ttgggaatat 900 tattggcatc caatttaaaa gaaatccaac aacatggaaa caacaagaaa tttggtatga 960 gtattgatga ggtgattgaa gagtgtaaac tcttctattt tgctgggcaa gagactactt 1020 catctttact tgtatggact atgattttgt tgtgcaaata tcctaattgg caagataaag 1080 ctagagaaga ggttttgcaa gtgtttggga gtagggaagt tgactatgac aagttgaatc 1140 agctaaaaat agtaactatg atcttaaacg aggtcttaag gttgtatcca gcaggatatg 1200 tgattaatcg aatggtaaac aaagaaacaa agttagggaa tttgtgttta ccagccggcg 1260 tacagctcgt gttaccaaca atgttgttgc aacatgatac tgaaatatgg ggagatgatg 1320 caatggagtt caatccagag agatttagtg atggaatatc caaagcaaca aaaggaaaac 1380 ttgtgttttt tccatttagt tggggtccaa gaatatgtat tgggcaaaat tttgctatgt 1440 tagaggctaa aatggcaatg gctatgattc tgaaaaccta tgcatttgaa ctctctccat 1500 cttatgctca tgctcctcat ccactactac ttcaacctca atatggtgct caattaattt 1560 tgtacaagtt gtagatatgg tcaatctgga acttgttatg gaacttttat catcgtaatc 1620 aaccatattg aggg 1634 220 508 PRT NICOTIANATABACUM 220 Met Glu Ile Pro Tyr Tyr Ser Leu Lys Ile Ala Ile Ser Ser Phe Ala 1 5 10 15 Ile Ile Phe Val Leu Arg Trp Ala Trp Lys Ile Leu Asn Tyr Val Trp 20 25 30 Leu Lys Pro Lys Glu Leu Glu Lys Tyr Leu Arg Gln Gln Gly Phe Lys 35 40 45 Gly Asn Ser Tyr Lys Phe Leu Phe Gly Asp Met Lys Glu Thr Lys Lys 50 55 60 Met Gly Glu Glu Ala Met Ser Lys Pro Ile Asn Phe Ser His Asp Met 65 70 75 80 Ile Trp Pro Arg Val Met Pro Phe Ile His Lys Thr Ile Thr Asn Tyr 85 90 95 Gly Lys Asn Cys Ile Val Trp Phe Gly Pro Arg Pro Ala Val Leu Ile 100 105 110 Thr Asp Pro Glu Leu Val Lys Glu Pro Leu Gly Asn Pro Leu Thr Lys 115 120 125 Leu Ala Ala Thr Gly Ile Ala Gly Tyr Glu Thr Asp Lys Trp Ala Thr 130 135 140 His Arg Arg Leu Leu Asn Pro Ala Phe His Leu Asp Lys Leu Lys His 145 150 155 160 Met Leu Pro Ala Phe Gln Phe Thr Ala Ser Glu Met Leu Ser Lys Leu 165 170 175 Glu Lys Val Val Ser Pro Asn Gly Thr Glu Ile Asp Val Trp Pro Tyr 180 185 190 Leu Gln Thr Leu Thr Ser Asp Ala Ile Ser Arg Thr Ala Phe Gly Ser 195 200 205 Ser Tyr Glu Glu Gly Arg Lys Ile Phe Asp Leu Gln Lys Glu Gln Leu 210 215 220 Ser Leu Ile Leu Glu Val Ser Arg Thr Ile Tyr Ile Pro Gly Trp Arg 225 230 235 240 Phe Leu Pro Thr Lys Arg Asn Lys Arg Met Lys Gln Ile Phe Asn Glu 245 250 255 Val Arg Ala Leu Val Phe Gly Ile Ile Lys Lys Arg Met Ser Met Ile 260 265 270 Glu Asn Gly Glu Ala Pro Asp Asp Leu Leu Gly Ile Leu Leu Ala Ser 275 280 285 Asn Leu Lys Glu Ile Gln Gln His Gly Asn Asn Lys Lys Phe Gly Met 290 295 300 Ser Ile Asp Glu Val Ile Glu Glu Cys Lys Leu Phe Tyr Phe Ala Gly 305 310 315 320 Gln Glu Thr Thr Ser Ser Leu Leu Val Trp Thr Met Ile Leu Leu Cys 325 330 335 Lys Tyr Pro Asn Trp Gln Asp Lys Ala Arg Glu Glu Val Leu Gln Val 340 345 350 Phe Gly Ser Arg Glu Val Asp Tyr Asp Lys Leu Asn Gln Leu Lys Ile 355 360 365 Val Thr Met Ile Leu Asn Glu Val Leu Arg Leu Tyr Pro Ala Gly Tyr 370 375 380 Val Ile Asn Arg Met Val Asn Lys Glu Thr Lys Leu Gly Asn Leu Cys 385 390 395 400 Leu Pro Ala Gly Val Gln Leu Val Leu Pro Thr Met Leu Leu Gln His 405 410 415 Asp Thr Glu Ile Trp Gly Asp Asp Ala Met Glu Phe Asn Pro Glu Arg 420 425 430 Phe Ser Asp Gly Ile Ser Lys Ala Thr Lys Gly Lys Leu Val Phe Phe 435 440 445 Pro Phe Ser Trp Gly Pro Arg Ile Cys Ile Gly Gln Asn Phe Ala Met 450 455 460 Leu Glu Ala Lys Met Ala Met Ala Met Ile Leu Lys Thr Tyr Ala Phe 465 470 475 480 Glu Leu Ser Pro Ser Tyr Ala His Ala Pro His Pro Leu Leu Leu Gln 485 490 495 Pro Gln Tyr Gly Ala Gln Leu Ile Leu Tyr Lys Leu 500 505 221 1593 DNA NICOTIANATABACUM 221 atggtttttc ccatagaagc ctttgtagga ctagtaacct tcacatttct cttatacttc 60 ctatggacaa aaaaatctca aaaacttcca aaacccttac caccgaaaat ccccggagga 120 tggccggtaa tcggccacct ttttcacttc aataacgacg gcgacgaccg tccattagct 180 cgaaaactcg gagacttagc tgataaatac ggccccgttt tcacttttcg gctaggtctt 240 ccccttgtgc tagttgtaag cagttacgaa gctacaaaag attgcttctc tacaaatgac 300 gccattttct ccaatcgtcc agcttttctt tacggcgaat accttggcta caataataca 360 atgctttttc tagcaaatta cggaccttac tggcgaaaaa atcgtaaatt agtcattcag 420 gaagttctct ctgctagtcg tctcgaaaaa ttcaaacaag tgagattcac cagaattcaa 480 acgagcatta agaatttata cactcgaatt aatggaaatt cgagtacgat aaatctaact 540 gattggttag aagaattgaa ttttggtctg atcgtgaaaa tgatcgctgg gaaaaattat 600 gaatccggta aaggagatga acaagtggaa agatttaaga atgcgtttaa ggattttatg 660 gttttatcaa tggaatttgt attatgggat gcatttccaa ttccattatt taaatgggtg 720 gattttcaag gtcatattaa gacaatgaaa aggacattta aggatataga ttctgttttt 780 cagaactggt tagaggaaca tattaataaa agagaaaaaa tggaggttgg tgcagaaggg 840 aatgaacaag atttcattga tgtggtgctt tcaaaattga gtaaagaata tcttgatgaa 900 ggttactctc gtgatactgt cattaaagca acagttttta gtttggtctt ggatgcagca 960 gacacagttg ctcttcacat aaattgggga atgacattat tgataaacaa tcaaaatgcc 1020 ttgatgaaag cacaagaaga gatagacaca aaagttggta aggatagatg ggtagaagag 1080 agtgatatta aggatttagt atacctccaa gctattgtta aaaaggtgtt acgattatat 1140 ccaccaggac ctttgttagt accacatgaa aatgtaaagg attgtgttgt tagtggatat 1200 cacattccta aagggactag attattcgca aacgtcatga aactgcagcg cgatcctaaa 1260 ctcttgtcaa atcctgataa gttcgatcca gagagattca tcgctggtga tattgacttc 1320 cgtggtcacc actatgagtt tatcccatct ggttctggaa gacgatcttg tccggggatg 1380 acttatgcat tgcaagtgga acacctaaca atggcacatt taatccaggg tttcaattac 1440 aaaactccaa atgacgaggt cttggatatg aaggaaggtg caggcataac aatacgtaag 1500 gtaaatccag tggaattgat aataacgcct cgcttggcac ctgagcttta ctaaaaccta 1560 agatctttca tcttggttga tcattgttta ata 1593 222 517 PRT NICOTIANATABACUM 222 Met Val Phe Pro Ile Glu Ala Phe Val Gly Leu Val Thr Phe Thr Phe 1 5 10 15 Leu Leu Tyr Phe Leu Trp Thr Lys Lys Ser Gln Lys Leu Pro Lys Pro 20 25 30 Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe 35 40 45 His Phe Asn Asn Asp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly 50 55 60 Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu 65 70 75 80 Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Thr Lys Asp Cys Phe 85 90 95 Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr Gly 100 105 110 Glu Tyr Leu Gly Tyr Asn Asn Thr Met Leu Phe Leu Ala Asn Tyr Gly 115 120 125 Pro Tyr Trp Arg Lys Asn Arg Lys Leu Val Ile Gln Glu Val Leu Ser 130 135 140 Ala Ser Arg Leu Glu Lys Phe Lys Gln Val Arg Phe Thr Arg Ile Gln 145 150 155 160 Thr Ser Ile Lys Asn Leu Tyr Thr Arg Ile Asn Gly Asn Ser Ser Thr 165 170 175 Ile Asn Leu Thr Asp Trp Leu Glu Glu Leu Asn Phe Gly Leu Ile Val 180 185 190 Lys Met Ile Ala Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu Gln 195 200 205 Val Glu Arg Phe Lys Asn Ala Phe Lys Asp Phe Met Val Leu Ser Met 210 215 220 Glu Phe Val Leu Trp Asp Ala Phe Pro Ile Pro Leu Phe Lys Trp Val 225 230 235 240 Asp Phe Gln Gly His Ile Lys Thr Met Lys Arg Thr Phe Lys Asp Ile 245 250 255 Asp Ser Val Phe Gln Asn Trp Leu Glu Glu His Ile Asn Lys Arg Glu 260 265 270 Lys Met Glu Val Gly Ala Glu Gly Asn Glu Gln Asp Phe Ile Asp Val 275 280 285 Val Leu Ser Lys Leu Ser Lys Glu Tyr Leu Asp Glu Gly Tyr Ser Arg 290 295 300 Asp Thr Val Ile Lys Ala Thr Val Phe Ser Leu Val Leu Asp Ala Ala 305 310 315 320 Asp Thr Val Ala Leu His Ile Asn Trp Gly Met Thr Leu Leu Ile Asn 325 330 335 Asn Gln Asn Ala Leu Met Lys Ala Gln Glu Glu Ile Asp Thr Lys Val 340 345 350 Gly Lys Asp Arg Trp Val Glu Glu Ser Asp Ile Lys Asp Leu Val Tyr 355 360 365 Leu Gln Ala Ile Val Lys Lys Val Leu Arg Leu Tyr Pro Pro Gly Pro 370 375 380 Leu Leu Val Pro His Glu Asn Val Lys Asp Cys Val Val Ser Gly Tyr 385 390 395 400 His Ile Pro Lys Gly Thr Arg Leu Phe Ala Asn Val Met Lys Leu Gln 405 410 415 Arg Asp Pro Lys Leu Leu Ser Asn Pro Asp Lys Phe Asp Pro Glu Arg 420 425 430 Phe Ile Ala Gly Asp Ile Asp Phe Arg Gly His His Tyr Glu Phe Ile 435 440 445 Pro Ser Gly Ser Gly Arg Arg Ser Cys Pro Gly Met Thr Tyr Ala Leu 450 455 460 Gln Val Glu His Leu Thr Met Ala His Leu Ile Gln Gly Phe Asn Tyr 465 470 475 480 Lys Thr Pro Asn Asp Glu Val Leu Asp Met Lys Glu Gly Ala Gly Ile 485 490 495 Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile Ile Thr Pro Arg Leu 500 505 510 Ala Pro Glu Leu Tyr 515 223 1580 DNA NICOTIANATABACUM 223 atgctttctc ccatagaagc cattgtagga ctagtaacct tcacatttct cttcttcttc 60 ctatggacaa aaaaatctca aaaaccttca aaacccttac caccgaaaat ccccggagga 120 tggccggtaa tcggccatct tttccacttc aatgacgacg gcgacgaccg tccattagct 180 cgaaaactcg gagacttagc tgacaaatac ggccccgttt tcacttttcg gctaggcctt 240 ccccttgtct tagttgtaag cagttacgaa gctgtaaaag actgtttctc cacaaatgac 300 gccatttttt ccaatcgtcc agcttttctt tacggcgatt accttggcta caataatgcc 360 atgctatttt tggccaatta cggaccttac tggcgaaaaa atcgaaaatt agttattcag 420 gaagttctct ccgctagtcg tctcgaaaaa ttcaaacacg tgagatttgc aagaattcaa 480 gcgagcatga agaatttata tactcgaatt gatggaaatt cgagtacgat aaatttaact 540 gattggttag aagaattgaa ttttggtctg atcgtgaaga tgatcgctgg aaaaaattat 600 gaatccggta aaggagatga acaagtggag agatttaaga aagcgtttaa ggattttatg 660 attttatcaa tggagtttgt gttatgggat gcatttccaa ttccattatt taaatgggtg 720 gattttcaag ggcatgttaa ggctatgaaa aggactttta aagatataga ttctgttttt 780 cagaattggt tagaggaaca tattaataaa agagaaaaaa tggaggttaa tgcagaaggg 840 aatgaacaag atttcattga tgtggtgctt tcaaaaatga gtaatgaata tcttggtgaa 900 ggttactctc gtgatactgt cattgaagca acggtgttta gtttggtctt ggatgcagca 960 gacacagttg ctcttcacat aaattgggga atggcattat tgataaacaa tcaaaaggcc 1020 ttgacgaaag cacaagaaga gatagacaca aaagtttgta aggacagatg ggtagaagag 1080 agtgatatta aggatttggt atacctccaa gctattgtta aagaagtgtt acgattatat 1140 ccaccaggac ctttgttagt accacacgaa aatgtagaag attgtgttgt tagtggatat 1200 cacattccta aagggacaag attattcgca aacgtcatga aactgcaacg tgatcctaaa 1260 ctctggtctg atcctgatac tttcgatcca gagagattca ttgctactga tattgacttt 1320 cgtggtcagt actataagta tatcccgttt ggtcctggaa gacgatcttg tccagggatg 1380 acttatgcat tgcaagtgga acacttaaca atggcacatt tgatccaagg tttcaattac 1440 agaactccaa atgacgagcc cttggatatg aaggaaggtg caggcataac tatacgtaag 1500 gtaaatcctg tggaactgat aatagcgcct cgcctggcac ctgagcttta ttaaaaccta 1560 agatgtttca tcttggttga 1580 224 517 PRT NICOTIANATABACUM 224 Met Leu Ser Pro Ile Glu Ala Ile Val Gly Leu Val Thr Phe Thr Phe 1 5 10 15 Leu Phe Phe Phe Leu Trp Thr Lys Lys Ser Gln Lys Pro Ser Lys Pro 20 25 30 Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe 35 40 45 His Phe Asn Asp Asp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly 50 55 60 Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu 65 70 75 80 Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Val Lys Asp Cys Phe 85 90 95 Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr Gly 100 105 110 Asp Tyr Leu Gly Tyr Asn Asn Ala Met Leu Phe Leu Ala Asn Tyr Gly 115 120 125 Pro Tyr Trp Arg Lys Asn Arg Lys Leu Val Ile Gln Glu Val Leu Ser 130 135 140 Ala Ser Arg Leu Glu Lys Phe Lys His Val Arg Phe Ala Arg Ile Gln 145 150 155 160 Ala Ser Met Lys Asn Leu Tyr Thr Arg Ile Asp Gly Asn Ser Ser Thr 165 170 175 Ile Asn Leu Thr Asp Trp Leu Glu Glu Leu Asn Phe Gly Leu Ile Val 180 185 190 Lys Met Ile Ala Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu Gln 195 200 205 Val Glu Arg Phe Lys Lys Ala Phe Lys Asp Phe Met Ile Leu Ser Met 210 215 220 Glu Phe Val Leu Trp Asp Ala Phe Pro Ile Pro Leu Phe Lys Trp Val 225 230 235 240 Asp Phe Gln Gly His Val Lys Ala Met Lys Arg Thr Phe Lys Asp Ile 245 250 255 Asp Ser Val Phe Gln Asn Trp Leu Glu Glu His Ile Asn Lys Arg Glu 260 265 270 Lys Met Glu Val Asn Ala Glu Gly Asn Glu Gln Asp Phe Ile Asp Val 275 280 285 Val Leu Ser Lys Met Ser Asn Glu Tyr Leu Gly Glu Gly Tyr Ser Arg 290 295 300 Asp Thr Val Ile Glu Ala Thr Val Phe Ser Leu Val Leu Asp Ala Ala 305 310 315 320 Asp Thr Val Ala Leu His Ile Asn Trp Gly Met Ala Leu Leu Ile Asn 325 330 335 Asn Gln Lys Ala Leu Thr Lys Ala Gln Glu Glu Ile Asp Thr Lys Val 340 345 350 Cys Lys Asp Arg Trp Val Glu Glu Ser Asp Ile Lys Asp Leu Val Tyr 355 360 365 Leu Gln Ala Ile Val Lys Glu Val Leu Arg Leu Tyr Pro Pro Gly Pro 370 375 380 Leu Leu Val Pro His Glu Asn Val Glu Asp Cys Val Val Ser Gly Tyr 385 390 395 400 His Ile Pro Lys Gly Thr Arg Leu Phe Ala Asn Val Met Lys Leu Gln 405 410 415 Arg Asp Pro Lys Leu Trp Ser Asp Pro Asp Thr Phe Asp Pro Glu Arg 420 425 430 Phe Ile Ala Thr Asp Ile Asp Phe Arg Gly Gln Tyr Tyr Lys Tyr Ile 435 440 445 Pro Phe Gly Pro Gly Arg Arg Ser Cys Pro Gly Met Thr Tyr Ala Leu 450 455 460 Gln Val Glu His Leu Thr Met Ala His Leu Ile Gln Gly Phe Asn Tyr 465 470 475 480 Arg Thr Pro Asn Asp Glu Pro Leu Asp Met Lys Glu Gly Ala Gly Ile 485 490 495 Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile Ile Ala Pro Arg Leu 500 505 510 Ala Pro Glu Leu Tyr 515 225 1639 DNA NICOTIANATABACUM 225 tttcatatac ctttagtact cttgaaattt tcaaataatg gtttatcttc tttctcccat 60 agaagccatt gtaggatttg taaccttttc atttctattc tactttctat ggaccaaaaa 120 acaatcaaaa atcttaaacc cactacctcc aaaaatccca ggtggatggc cagtaatcgg 180 ccatctcttt tatttcaaga acaatggcga tgaagatcgc catttttctc aaaaactcgg 240 tgacttagct gacaaatatg gtcccgtctt cactttccgg ttagggtttc gccgtttctt 300 ggcggtgagt agttatgaag ctatgaaaga atgcttcact accaatgata tccatttcgc 360 cgatcggcca tctttactct acggagaata cctttgctat aataacgcca tgcttgctgt 420 tgccaaatat ggcccttact ggaaaaaaaa tcgaaagtta gtcaatcaag aagttctctc 480 cgttagtcgg ctcgaaaaat tcaaacatgt tagattttct ataattcaga aaaatattaa 540 acaattgtat aattgtgatt caccaatggt gaagataaac cttagtgatt ggatagataa 600 attgacattc gacatcattt tgaaaatggt tgttgggaag aactataata atggacatgg 660 agaaatactc aaagttgctt ttcagaaatt catggttcaa gctatggaga tggagctcta 720 tgatgttttt cacattccat ttttcaagtg gttggatctt acagggaata ttaaggctat 780 gaaacaaact ttcaaagaca ttgataatat tatccaaggt tggttagatg agcacattaa 840 gaagagagaa acaaaggatg ttggaggtga aaacgaacaa gattttatag atgtggtgct 900 ttccaagatg agcgacgaac atcttggcga gggttactct catgacacaa ccatcaaagc 960 aactgtattc actttggtct tggatgcaac agacacactt gcacttcata taaagtgggt 1020 aatggcgtta atgataaaca ataagcatgt catgaagaaa gcacaagaag agatggacac 1080 aattgttggt agagatagat gggtagaaga gagtgatatc aagaatttgg tgtatctcca 1140 agcaattgtc aaagaagtat tacgattaca tccacccgca cctttgtcag tgcaacacct 1200 atctgtagaa gattgtgttg tcaatgggta ccatattcct aaggggactg cactacttac 1260 caatattatg aaactacagc gagatcctca aacatggcca aatcctgata aattcgatcc 1320 agagagattc ctgacgactc atgctactat tgactaccgc gggcagcact atgagtcgat 1380 cccctttggt acggggagac gagcttgtcc cgcgatgaat tattcattgc aagtggaaca 1440 cctttcaatt gctcatatga tccaaggttt cagttttgca actacgacca atgagccttt 1500 ggatatgaaa caaggtgtgg gtttaacttt accaaagaag actgatgttg aagtgctaat 1560 tacacctcgc cttcctccta cgctttatca atattaagat gttttgttgt cgggattcgt 1620 tctgatcaat ccctcaatg 1639 226 519 PRT NICOTIANATABACUM 226 Met Val Tyr Leu Leu Ser Pro Ile Glu Ala Ile Val Gly Phe Val Thr 1 5 10 15 Phe Ser Phe Leu Phe Tyr Phe Leu Trp Thr Lys Lys Gln Ser Lys Ile 20 25 30 Leu Asn Pro Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly 35 40 45 His Leu Phe Tyr Phe Lys Asn Asn Gly Asp Glu Asp Arg His Phe Ser 50 55 60 Gln Lys Leu Gly Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe 65 70 75 80 Arg Leu Gly Phe Arg Arg Phe Leu Ala Val Ser Ser Tyr Glu Ala Met 85 90 95 Lys Glu Cys Phe Thr Thr Asn Asp Ile His Phe Ala Asp Arg Pro Ser 100 105 110 Leu Leu Tyr Gly Glu Tyr Leu Cys Tyr Asn Asn Ala Met Leu Ala Val 115 120 125 Ala Lys Tyr Gly Pro Tyr Trp Lys Lys Asn Arg Lys Leu Val Asn Gln 130 135 140 Glu Val Leu Ser Val Ser Arg Leu Glu Lys Phe Lys His Val Arg Phe 145 150 155 160 Ser Ile Ile Gln Lys Asn Ile Lys Gln Leu Tyr Asn Cys Asp Ser Pro 165 170 175 Met Val Lys Ile Asn Leu Ser Asp Trp Ile Asp Lys Leu Thr Phe Asp 180 185 190 Ile Ile Leu Lys Met Val Val Gly Lys Asn Tyr Asn Asn Gly His Gly 195 200 205 Glu Ile Leu Lys Val Ala Phe Gln Lys Phe Met Val Gln Ala Met Glu 210 215 220 Met Glu Leu Tyr Asp Val Phe His Ile Pro Phe Phe Lys Trp Leu Asp 225 230 235 240 Leu Thr Gly Asn Ile Lys Ala Met Lys Gln Thr Phe Lys Asp Ile Asp 245 250 255 Asn Ile Ile Gln Gly Trp Leu Asp Glu His Ile Lys Lys Arg Glu Thr 260 265 270 Lys Asp Val Gly Gly Glu Asn Glu Gln Asp Phe Ile Asp Val Val Leu 275 280 285 Ser Lys Met Ser Asp Glu His Leu Gly Glu Gly Tyr Ser His Asp Thr 290 295 300 Thr Ile Lys Ala Thr Val Phe Thr Leu Val Leu Asp Ala Thr Asp Thr 305 310 315 320 Leu Ala Leu His Ile Lys Trp Val Met Ala Leu Met Ile Asn Asn Lys 325 330 335 His Val Met Lys Lys Ala Gln Glu Glu Met Asp Thr Ile Val Gly Arg 340 345 350 Asp Arg Trp Val Glu Glu Ser Asp Ile Lys Asn Leu Val Tyr Leu Gln 355 360 365 Ala Ile Val Lys Glu Val Leu Arg Leu His Pro Pro Ala Pro Leu Ser 370 375 380 Val Gln His Leu Ser Val Glu Asp Cys Val Val Asn Gly Tyr His Ile 385 390 395 400 Pro Lys Gly Thr Ala Leu Leu Thr Asn Ile Met Lys Leu Gln Arg Asp 405 410 415 Pro Gln Thr Trp Pro Asn Pro Asp Lys Phe Asp Pro Glu Arg Phe Leu 420 425 430 Thr Thr His Ala Thr Ile Asp Tyr Arg Gly Gln His Tyr Glu Ser Ile 435 440 445 Pro Phe Gly Thr Gly Arg Arg Ala Cys Pro Ala Met Asn Tyr Ser Leu 450 455 460 Gln Val Glu His Leu Ser Ile Ala His Met Ile Gln Gly Phe Ser Phe 465 470 475 480 Ala Thr Thr Thr Asn Glu Pro Leu Asp Met Lys Gln Gly Val Gly Leu 485 490 495 Thr Leu Pro Lys Lys Thr Asp Val Glu Val Leu Ile Thr Pro Arg Leu 500 505 510 Pro Pro Thr Leu Tyr Gln Tyr 515 227 1610 DNA NICOTIANATABACUM 227 ctttttctcc ccaaaaaaga gctcatttcc cttgtcccca aaaatggatc ttctcttact 60 agagaagacc ttaattggtc tcttctttgc cattttaatc gctataattg tctctagact 120 tcgttcaaag cgttttaagc ttcccccagg accaatccca gtaccagttt ttggtaattg 180 gcttcaagtt ggtgatgatt taaaccacag aaatcttact gattttgcca aaaaatttgg 240 tgatcttttc ttgttaagaa tgggccagcg taatttagtt gttgtgtcat ctcctgaatt 300 agctaaagaa gttttacaca cacaaggtgt tgaatttggt tcaagaacaa gaaatgttgt 360 atttgatatt tttactggaa aaggtcaaga tatggttttt actgtatatg gtgaacactg 420 gagaaaaatg aggagaatta tgactgtacc attttttact aataaagttg tgcagcaata 480 tagagggggg tgggagtttg aagtggcaag tgtaattgag gatgtgaaga aaaatcctga 540 atctgctact aatgggattg tattaaggag gagattacaa ttgatgatgt ataataatat 600 gtttaggatt atgtttgata ggagatttga gagtgaagat gatcctttgt ttgttaagct 660 taaggctttg aatggtgaaa ggagtagatt ggctcagagt tttgagtata attatggtga 720 ttttattccc attttgaggc cttttttgag aggttatttg aagatctgta aagaagttaa 780 ggagaagagg ctgcagcttt tcaaagatta ctttgttgat gaaagaaaga agctttcaaa 840 taccaagagc ttggacagca atgctctgaa atgtgcgatt gatcacattc ttgaggctca 900 acagaagggg gagatcaatg aggacaacgt tctttacatt gttgaaaaca tcaatgttgc 960 tgctatagaa accacattat ggtcaattga gtggggtatc gccgagttag tcaaccaccc 1020 tcacatccaa aagaaactcc gcgacgagat tgacacagtt cttggcccag gagtgcaagt 1080 gactgaacca gacacccaca agcttccata ccttcaggct gtgatcaagg agacgcttcg 1140 tctccgtatg gcaattcctc tattagtccc acacatgaac cttcacgatg caaagcttgg 1200 cgggtttgat attccagcag agagcaaaat cttggttaac gcttggtggc tagctaacaa 1260 cccggctcat tggaagaaac ccgaagagtt cagacccgag aggttcttcg aagaggagaa 1320 gcacgttgag gccaatggca atgacttcag atatcttccg tttggcgttg gtaggaggag 1380 ttgccctgga attatacttg cattgccaat tcttggcatt actttgggac gtttggttca 1440 gaactttgag ctgttgcctc ctccaggcca gtcgaagctc gacaccacag agaaaggtgg 1500 acagttcagt ctccatattt tgaagcattc caccattgtg ttgaaaccaa ggtcttgctg 1560 aactttctga tcctaatcaa ttaaggggtt gaagaaattt tataattatg 1610 228 505 PRT NICOTIANATABACUM 228 Met Asp Leu Leu Leu Leu Glu Lys Thr Leu Ile Gly Leu Phe Phe Ala 1 5 10 15 Ile Leu Ile Ala Ile Ile Val Ser Arg Leu Arg Ser Lys Arg Phe Lys 20 25 30 Leu Pro Pro Gly Pro Ile Pro Val Pro Val Phe Gly Asn Trp Leu Gln 35 40 45 Val Gly Asp Asp Leu Asn His Arg Asn Leu Thr Asp Phe Ala Lys Lys 50 55 60 Phe Gly Asp Leu Phe Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val 65 70 75 80 Val Ser Ser Pro Glu Leu Ala Lys Glu Val Leu His Thr Gln Gly Val 85 90 95 Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly 100 105 110 Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp Arg Lys 115 120 125 Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln 130 135 140 Gln Tyr Arg Gly Gly Trp Glu Phe Glu Val Ala Ser Val Ile Glu Asp 145 150 155 160 Val Lys Lys Asn Pro Glu Ser Ala Thr Asn Gly Ile Val Leu Arg Arg 165 170 175 Arg Leu Gln Leu Met Met Tyr Asn Asn Met Phe Arg Ile Met Phe Asp 180 185 190 Arg Arg Phe Glu Ser Glu Asp Asp Pro Leu Phe Val Lys Leu Lys Ala 195 200 205 Leu Asn Gly Glu Arg Ser Arg Leu Ala Gln Ser Phe Glu Tyr Asn Tyr 210 215 220 Gly Asp Phe Ile Pro Ile Leu Arg Pro Phe Leu Arg Gly Tyr Leu Lys 225 230 235 240 Ile Cys Lys Glu Val Lys Glu Lys Arg Leu Gln Leu Phe Lys Asp Tyr 245 250 255 Phe Val Asp Glu Arg Lys Lys Leu Ser Asn Thr Lys Ser Leu Asp Ser 260 265 270 Asn Ala Leu Lys Cys Ala Ile Asp His Ile Leu Glu Ala Gln Gln Lys 275 280 285 Gly Glu Ile Asn Glu Asp Asn Val Leu Tyr Ile Val Glu Asn Ile Asn 290 295 300 Val Ala Ala Ile Glu Thr Thr Leu Trp Ser Ile Glu Trp Gly Ile Ala 305 310 315 320 Glu Leu Val Asn His Pro His Ile Gln Lys Lys Leu Arg Asp Glu Ile 325 330 335 Asp Thr Val Leu Gly Pro Gly Val Gln Val Thr Glu Pro Asp Thr His 340 345 350 Lys Leu Pro Tyr Leu Gln Ala Val Ile Lys Glu Thr Leu Arg Leu Arg 355 360 365 Met Ala Ile Pro Leu Leu Val Pro His Met Asn Leu His Asp Ala Lys 370 375 380 Leu Gly Gly Phe Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn Ala 385 390 395 400 Trp Trp Leu Ala Asn Asn Pro Ala His Trp Lys Lys Pro Glu Glu Phe 405 410 415 Arg Pro Glu Arg Phe Phe Glu Glu Glu Lys His Val Glu Ala Asn Gly 420 425 430 Asn Asp Phe Arg Tyr Leu Pro Phe Gly Val Gly Arg Arg Ser Cys Pro 435 440 445 Gly Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Leu Gly Arg Leu 450 455 460 Val Gln Asn Phe Glu Leu Leu Pro Pro Pro Gly Gln Ser Lys Leu Asp 465 470 475 480 Thr Thr Glu Lys Gly Gly Gln Phe Ser Leu His Ile Leu Lys His Ser 485 490 495 Thr Ile Val Leu Lys Pro Arg Ser Cys 500 505 229 1595 DNA NICOTIANATABACUM 229 aacaataaaa atggagacat tatttaacat caaagttgca gtttcattag taattgtgat 60 aatttttctg agatgggtat ggaaattctt gaattgggtg tggattcaac caaagaaaat 120 ggaaaaaaga ctaaaaatgg aaggtttcaa aggaagctca tataagctat tatttggaga 180 tatgaaagaa ataaatacaa tggttgaaga agccaaaacc aagcctatga attttaccaa 240 tgattatgtg gctagagtct tgcctcactt cacaaagttg atgctccaat atggcaagaa 300 tagctttatg tggttagggc caaaaccaac aatgtttatc acagaccctg aactaataag 360 ggagatcttg tcaaaaagtt acatatacca ggagattcaa ggcaatccaa tcactaagtt 420 gctagcacaa ggactagtaa gttatgaagc agagaaatgg gctaagcata gaaaaattat 480 caatcctgca tttcaccttg acaagttgaa gcatatgcta ccatcattct acttgagttg 540 ttgtgacatg ctcagaaaat gggaaagtat agcttcatca gagggatcag aaatagacgt 600 gtggcctttt ctggaaacgt tgacaagcga tgctatttca agaacagctt ttggtagtaa 660 ctatgaagac gggagacaga tatttgagct tcaaaaagaa caagctgagt tgattttaca 720 agcagcgcga tggctttaca tccccggatg gaggtttgtg ccaacaaaga ggaacaagag 780 gatgaagcaa atcgctaaag aagtacgatc attagtgttg ggaataatca ataagagaat 840 aagggaaatg aaagcagggg aagctgcaaa agatgactta ctgggaatac tattggaatc 900 taatttcaaa gaaatccaaa tgcacggaaa caagaacttt ggcatgacta tcgacgaagt 960 gattgaagag tgcaagttat tttactttgc tgggcaagaa actacttcag ttttgcttgt 1020 ttggactttg attttactga gtaagcatgt cgattggcaa gaaagagcta gagaagaagt 1080 tcatcaagtc tttggaagta acaaacctga ttatgacgca ttgaatcagt tgaaagttgt 1140 aacgatgata ttcaacgagg ttttaaggtt gtacccaccg ggaattacca taagtcgaac 1200 tgtacacgag gataccaaat tagggaactt gtcattgcca gcagggatac agcttgtgtt 1260 acctgcaatt tggttgcatc atgacaatga aatatgggga gatgatgcaa aggagttcaa 1320 accagagagg tttagtgaag gagttaataa agcaacaaag ggtaaatttg catattttcc 1380 atttagttgg ggaccaagaa tatgtgttgg actgaatttt gcaatgttag aggcaaaaat 1440 ggcacttgca ttgattctac aacactatgc ttttgagctc tctccatctt atgcacatgc 1500 tcctcataca attatcactc tgcaacctca acatggtgct cctttgattt tgcgcaagct 1560 gtagcgcgga tatattgatt ggttatctac tgtag 1595 230 517 PRT NICOTIANATABACUM 230 Met Glu Thr Leu Phe Asn Ile Lys Val Ala Val Ser Leu Val Ile Val 1 5 10 15 Ile Ile Phe Leu Arg Trp Val Trp Lys Phe Leu Asn Trp Val Trp Ile 20 25 30 Gln Pro Lys Lys Met Glu Lys Arg Leu Lys Met Glu Gly Phe Lys Gly 35 40 45 Ser Ser Tyr Lys Leu Leu Phe Gly Asp Met Lys Glu Ile Asn Thr Met 50 55 60 Val Glu Glu Ala Lys Thr Lys Pro Met Asn Phe Thr Asn Asp Tyr Val 65 70 75 80 Ala Arg Val Leu Pro His Phe Thr Lys Leu Met Leu Gln Tyr Gly Lys 85 90 95 Asn Ser Phe Met Trp Leu Gly Pro Lys Pro Thr Met Phe Ile Thr Asp 100 105 110 Pro Glu Leu Ile Arg Glu Ile Leu Ser Lys Ser Tyr Ile Tyr Gln Glu 115 120 125 Ile Gln Gly Asn Pro Ile Thr Lys Leu Leu Ala Gln Gly Leu Val Ser 130 135 140 Tyr Glu Ala Glu Lys Trp Ala Lys His Arg Lys Ile Ile Asn Pro Ala 145 150 155 160 Phe His Leu Asp Lys Leu Lys His Met Leu Pro Ser Phe Tyr Leu Ser 165 170 175 Cys Cys Asp Met Leu Arg Lys Trp Glu Ser Ile Ala Ser Ser Glu Gly 180 185 190 Ser Glu Ile Asp Val Trp Pro Phe Leu Glu Thr Leu Thr Ser Asp Ala 195 200 205 Ile Ser Arg Thr Ala Phe Gly Ser Asn Tyr Glu Asp Gly Arg Gln Ile 210 215 220 Phe Glu Leu Gln Lys Glu Gln Ala Glu Leu Ile Leu Gln Ala Ala Arg 225 230 235 240 Trp Leu Tyr Ile Pro Gly Trp Arg Phe Val Pro Thr Lys Arg Asn Lys 245 250 255 Arg Met Lys Gln Ile Ala Lys Glu Val Arg Ser Leu Val Leu Gly Ile 260 265 270 Ile Asn Lys Arg Ile Arg Glu Met Lys Ala Gly Glu Ala Ala Lys Asp 275 280 285 Asp Leu Leu Gly Ile Leu Leu Glu Ser Asn Phe Lys Glu Ile Gln Met 290 295 300 His Gly Asn Lys Asn Phe Gly Met Thr Ile Asp Glu Val Ile Glu Glu 305 310 315 320 Cys Lys Leu Phe Tyr Phe Ala Gly Gln Glu Thr Thr Ser Val Leu Leu 325 330 335 Val Trp Thr Leu Ile Leu Leu Ser Lys His Val Asp Trp Gln Glu Arg 340 345 350 Ala Arg Glu Glu Val His Gln Val Phe Gly Ser Asn Lys Pro Asp Tyr 355 360 365 Asp Ala Leu Asn Gln Leu Lys Val Val Thr Met Ile Phe Asn Glu Val 370 375 380 Leu Arg Leu Tyr Pro Pro Gly Ile Thr Ile Ser Arg Thr Val His Glu 385 390 395 400 Asp Thr Lys Leu Gly Asn Leu Ser Leu Pro Ala Gly Ile Gln Leu Val 405 410 415 Leu Pro Ala Ile Trp Leu His His Asp Asn Glu Ile Trp Gly Asp Asp 420 425 430 Ala Lys Glu Phe Lys Pro Glu Arg Phe Ser Glu Gly Val Asn Lys Ala 435 440 445 Thr Lys Gly Lys Phe Ala Tyr Phe Pro Phe Ser Trp Gly Pro Arg Ile 450 455 460 Cys Val Gly Leu Asn Phe Ala Met Leu Glu Ala Lys Met Ala Leu Ala 465 470 475 480 Leu Ile Leu Gln His Tyr Ala Phe Glu Leu Ser Pro Ser Tyr Ala His 485 490 495 Ala Pro His Thr Ile Ile Thr Leu Gln Pro Gln His Gly Ala Pro Leu 500 505 510 Ile Leu Arg Lys Leu 515 231 1673 DNA NICOTIANATABACUM 231 atatgcaact gagatttgaa gaataccaac taaccaaaat gcagttcttc agcttggttt 60 ccattttcct atttctatct ttcctctttt tgttaaggat atggaagaac tccaatagcc 120 aaagcaaaaa gttgccacca ggtccatgga aactaccaat actaggaagt atgcttcata 180 tggttggtgg actaccacac catgtcctta gagatttagc caaaaaatat ggaccactta 240 tgcaccttca attaggtgaa gtttctgcgg ttgtggttac ttctcctgat acggcaaaag 300 aagtattaaa aactcatgac atcgcttttg cgtctaggcc tagccttttg gccccggaga 360 ttgtctgtta caataggtct gatctagcct tttgccccta tggcgactat tggagacaaa 420 tgcgtaaaat atgtgtcttg gaagtgctca gtgccaagaa tgttcggaca tttagctcta 480 ttaggcggaa tgaagttctt cgtctcatta attttatccg gtcatcttct ggtgaaccta 540 ttaatgttac ggaaaggatc tttttgttca caagctccat gacatgtaga tcagcgtttg 600 ggcaagtgtt caaagagcaa gacaaattta tacaactaat taaagaagtg atactcttag 660 caggagggtt tgatgtggct gacatattcc cttcactgaa gtttcttcat gtgctcagtg 720 gaatgaaggg taagattatg aatgcacacc ataaggtaga tgccattgtt gagaatgtca 780 tcaatgagca caagaaaaat cttgcaattg ggaaaactaa tggagcgtta ggaggtgaag 840 atttaattga tgttcttcta agacttatga atgatggagg ccttcaattt cctatcacca 900 acgacaacat caaagccata atttttgaca tgtttgctgc cgggacagag acttcatcgt 960 caacaattgt gtgggctatg gtagaaatgg tgaaaaatcc agccgtattc gcgaaagctc 1020 aagcagaagt aagagaagca tttagaggaa aagaaacttt cgatgaaaat gatgtggagg 1080 agctaaacta cctaaagtta gtaataaaag aaactctaag acttcatcca ccggttccac 1140 ttttgctccc aagagaatgt agggaagaga caaatataaa cggctacact attcctgtaa 1200 agaccaaagt catggttaat gtttgggctt tgggaagaga tccaaaatat tggaatgacg 1260 cagaaacttt tatgccagag agatttgagc agtgctctaa ggattttgtt ggtaataatt 1320 ttgaatatct tccatttggt ggcggaagga ggatttgtcc tgggatttcg tttggcttag 1380 ctaatgctta tttgccattg gctcaattac tatatcactt cgattggaaa ctccctgctg 1440 gaatcgaacc aagcgacttg gacttgactg agttggttgg agtaactgcc gctagaaaaa 1500 gtgaccttta cttggttgcg actccttatc aacctcctca aaagtgattt aatggtttca 1560 agtttttatt tcctagcaaa ccccactatt gtcctatctt tcttttggtg ttttcggttt 1620 tatctactct aatacatgca tcttttacca tataggaatg taccatgttg tcg 1673 232 514 PRT NICOTIANATABACUM 232 Met Gln Leu Arg Phe Glu Glu Tyr Gln Leu Thr Lys Met Gln Phe Phe 1 5 10 15 Ser Leu Val Ser Ile Phe Leu Phe Leu Ser Phe Leu Phe Leu Leu Arg 20 25 30 Ile Trp Lys Asn Ser Asn Ser Gln Ser Lys Lys Leu Pro Pro Gly Pro 35 40 45 Trp Lys Leu Pro Ile Leu Gly Ser Met Leu His Met Val Gly Gly Leu 50 55 60 Pro His His Val Leu Arg Asp Leu Ala Lys Lys Tyr Gly Pro Leu Met 65 70 75 80 His Leu Gln Leu Gly Glu Val Ser Ala Val Val Val Thr Ser Pro Asp 85 90 95 Thr Ala Lys Glu Val Leu Lys Thr His Asp Ile Ala Phe Ala Ser Arg 100 105 110 Pro Ser Leu Leu Ala Pro Glu Ile Val Cys Tyr Asn Arg Ser Asp Leu 115 120 125 Ala Phe Cys Pro Tyr Gly Asp Tyr Trp Arg Gln Met Arg Lys Ile Cys 130 135 140 Val Leu Glu Val Leu Ser Ala Lys Asn Val Arg Thr Phe Ser Ser Ile 145 150 155 160 Arg Arg Asn Glu Val Leu Arg Leu Ile Asn Phe Ile Arg Ser Ser Ser 165 170 175 Gly Glu Pro Ile Asn Val Thr Glu Arg Ile Phe Leu Phe Thr Ser Ser 180 185 190 Met Thr Cys Arg Ser Ala Phe Gly Gln Val Phe Lys Glu Gln Asp Lys 195 200 205 Phe Ile Gln Leu Ile Lys Glu Val Ile Leu Leu Ala Gly Gly Phe Asp 210 215 220 Val Ala Asp Ile Phe Pro Ser Leu Lys Phe Leu His Val Leu Ser Gly 225 230 235 240 Met Lys Gly Lys Ile Met Asn Ala His His Lys Val Asp Ala Ile Val 245 250 255 Glu Asn Val Ile Asn Glu His Lys Lys Asn Leu Ala Ile Gly Lys Thr 260 265 270 Asn Gly Ala Leu Gly Gly Glu Asp Leu Ile Asp Val Leu Leu Arg Leu 275 280 285 Met Asn Asp Gly Gly Leu Gln Phe Pro Ile Thr Asn Asp Asn Ile Lys 290 295 300 Ala Ile Ile Phe Asp Met Phe Ala Ala Gly Thr Glu Thr Ser Ser Ser 305 310 315 320 Thr Ile Val Trp Ala Met Val Glu Met Val Lys Asn Pro Ala Val Phe 325 330 335 Ala Lys Ala Gln Ala Glu Val Arg Glu Ala Phe Arg Gly Lys Glu Thr 340 345 350 Phe Asp Glu Asn Asp Val Glu Glu Leu Asn Tyr Leu Lys Leu Val Ile 355 360 365 Lys Glu Thr Leu Arg Leu His Pro Pro Val Pro Leu Leu Leu Pro Arg 370 375 380 Glu Cys Arg Glu Glu Thr Asn Ile Asn Gly Tyr Thr Ile Pro Val Lys 385 390 395 400 Thr Lys Val Met Val Asn Val Trp Ala Leu Gly Arg Asp Pro Lys Tyr 405 410 415 Trp Asn Asp Ala Glu Thr Phe Met Pro Glu Arg Phe Glu Gln Cys Ser 420 425 430 Lys Asp Phe Val Gly Asn Asn Phe Glu Tyr Leu Pro Phe Gly Gly Gly 435 440 445 Arg Arg Ile Cys Pro Gly Ile Ser Phe Gly Leu Ala Asn Ala Tyr Leu 450 455 460 Pro Leu Ala Gln Leu Leu Tyr His Phe Asp Trp Lys Leu Pro Ala Gly 465 470 475 480 Ile Glu Pro Ser Asp Leu Asp Leu Thr Glu Leu Val Gly Val Thr Ala 485 490 495 Ala Arg Lys Ser Asp Leu Tyr Leu Val Ala Thr Pro Tyr Gln Pro Pro 500 505 510 Gln Lys 233 1601 DNA NICOTIANATABACUM 233 gaattatttc acgtgttgta ttccttgtct atgataggaa gctcgttacc tcagcgtaca 60 aaccccaaat aaaaaatgaa tttccttgtg gtgttagctt ctctctttct ctttgtgttc 120 ctaatgagga taagcaaagc aaaaaagctc cctccaggtc caaggaaact gcctataata 180 ggaaaccttc atcaaattgg aaaattacct catcgttcac ttcaaaaact ttctaatgaa 240 tatggggatt tcattttctt gcaattaggt tctgtaccga ctgtggttgt ctcctcagct 300 gacattgccc gagagatctt tagaactcac gaccttgttt tctcaggccg tcctgcttta 360 tatgctgcca gaaaactttc ctacaattgc tacaacgttt catttgcacc ctatggtaat 420 tactggagag aggctcggaa aattctagtg ttggagttgc taagtacaaa gagagtacaa 480 agtttcgagg caattcgaga cgaggaagta agtagcttgg ttcaaattat ctgtagttcc 540 ttgagctcac ctgttaacat aagcacatta gcactatcct tggcaaataa cgttgtttgt 600 cgagtggctt ttgggaaagg gagtgctgaa ggaggaaatg attatgagga taggaagttt 660 aatgaaattc tatatgagac acaagaatta ttgggtgagt ttaacgttgc tgattatttt 720 cctcggatgg catggattaa caaaataaat gggtttgatg aacgattgga aaataatttt 780 agggaattgg ataagtttta tgacaaagta atagaagatc atcttaattc atgtagctgg 840 atgaaacaaa gggatgatga agacgttatt gatgtattgc ttcgaattca aaaggatcca 900 agccaagaaa ttcctctcaa agatgatcac attaagggcc ttcttgcgga tatattcata 960 gctggaactg atacatcatc aacaaccata gaatgggcaa tgtcagaact cataaaaaat 1020 ccaagagtct tgagaaaagc tcaagaggaa gttagagaag tttctaaggg aaaacaaaag 1080 gtccaagaaa gtgatctttg caaactagat tacttgaaat tggtcatcaa agaaaccttt 1140 agactacacc caccagtccc attactagtc cctcgagtaa caacagccag ctgcaaaata 1200 atggaatacg aaattccagt aaatacaaga gtcttcatca acgcgacagc aaatgggaca 1260 aatccaaaat actgggaaaa tccattgaca ttcttgccag agagattctt ggataaggag 1320 attgattaca gaggcaaaaa ttttgagttg ttgccatttg gggcagggag aagagggtgt 1380 ccaggaatta atttttcaat accacttgtt gagcttgcac ttgctaatct attgtttcat 1440 tataattggt cacttcctga agggatgcta gctaaggatg ttgatatgga agaagctttg 1500 gggattacca tgcacaagaa atctcccctt tgcttagtag cttctcatta tacttgttga 1560 gattttaaaa gattttagca tagctatata tagcttgaag t 1601 234 494 PRT NICOTIANATABACUM 234 Met Asn Phe Leu Val Val Leu Ala Ser Leu Phe Leu Phe Val Phe Leu 1 5 10 15 Met Arg Ile Ser Lys Ala Lys Lys Leu Pro Pro Gly Pro Arg Lys Leu 20 25 30 Pro Ile Ile Gly Asn Leu His Gln Ile Gly Lys Leu Pro His Arg Ser 35 40 45 Leu Gln Lys Leu Ser Asn Glu Tyr Gly Asp Phe Ile Phe Leu Gln Leu 50 55 60 Gly Ser Val Pro Thr Val Val Val Ser Ser Ala Asp Ile Ala Arg Glu 65 70 75 80 Ile Phe Arg Thr His Asp Leu Val Phe Ser Gly Arg Pro Ala Leu Tyr 85 90 95 Ala Ala Arg Lys Leu Ser Tyr Asn Cys Tyr Asn Val Ser Phe Ala Pro 100 105 110 Tyr Gly Asn Tyr Trp Arg Glu Ala Arg Lys Ile Leu Val Leu Glu Leu 115 120 125 Leu Ser Thr Lys Arg Val Gln Ser Phe Glu Ala Ile Arg Asp Glu Glu 130 135 140 Val Ser Ser Leu Val Gln Ile Ile Cys Ser Ser Leu Ser Ser Pro Val 145 150 155 160 Asn Ile Ser Thr Leu Ala Leu Ser Leu Ala Asn Asn Val Val Cys Arg 165 170 175 Val Ala Phe Gly Lys Gly Ser Ala Glu Gly Gly Asn Asp Tyr Glu Asp 180 185 190 Arg Lys Phe Asn Glu Ile Leu Tyr Glu Thr Gln Glu Leu Leu Gly Glu 195 200 205 Phe Asn Val Ala Asp Tyr Phe Pro Arg Met Ala Trp Ile Asn Lys Ile 210 215 220 Asn Gly Phe Asp Glu Arg Leu Glu Asn Asn Phe Arg Glu Leu Asp Lys 225 230 235 240 Phe Tyr Asp Lys Val Ile Glu Asp His Leu Asn Ser Cys Ser Trp Met 245 250 255 Lys Gln Arg Asp Asp Glu Asp Val Ile Asp Val Leu Leu Arg Ile Gln 260 265 270 Lys Asp Pro Ser Gln Glu Ile Pro Leu Lys Asp Asp His Ile Lys Gly 275 280 285 Leu Leu Ala Asp Ile Phe Ile Ala Gly Thr Asp Thr Ser Ser Thr Thr 290 295 300 Ile Glu Trp Ala Met Ser Glu Leu Ile Lys Asn Pro Arg Val Leu Arg 305 310 315 320 Lys Ala Gln Glu Glu Val Arg Glu Val Ser Lys Gly Lys Gln Lys Val 325 330 335 Gln Glu Ser Asp Leu Cys Lys Leu Asp Tyr Leu Lys Leu Val Ile Lys 340 345 350 Glu Thr Phe Arg Leu His Pro Pro Val Pro Leu Leu Val Pro Arg Val 355 360 365 Thr Thr Ala Ser Cys Lys Ile Met Glu Tyr Glu Ile Pro Val Asn Thr 370 375 380 Arg Val Phe Ile Asn Ala Thr Ala Asn Gly Thr Asn Pro Lys Tyr Trp 385 390 395 400 Glu Asn Pro Leu Thr Phe Leu Pro Glu Arg Phe Leu Asp Lys Glu Ile 405 410 415 Asp Tyr Arg Gly Lys Asn Phe Glu Leu Leu Pro Phe Gly Ala Gly Arg 420 425 430 Arg Gly Cys Pro Gly Ile Asn Phe Ser Ile Pro Leu Val Glu Leu Ala 435 440 445 Leu Ala Asn Leu Leu Phe His Tyr Asn Trp Ser Leu Pro Glu Gly Met 450 455 460 Leu Ala Lys Asp Val Asp Met Glu Glu Ala Leu Gly Ile Thr Met His 465 470 475 480 Lys Lys Ser Pro Leu Cys Leu Val Ala Ser His Tyr Thr Cys 485 490 235 1570 DNA NICOTIANATABACUM 235 caaagactaa aagatgtcgg tctttgcggt tatttcattc tttctacttc tgttttttct 60 tttcaaatca tatttgccct catcgaaaac aaagaaaaat tctccaccat ctccttcaaa 120 gcttccgtta atcggtcact tccacaaact aggcttacaa cctcaccgtt ctctacaaaa 180 actatcaaat gaacatggtc ccatgatgat gcttcaattc ggtagcgtac ctgtgcttat 240 cgcttcatca gctgaagctg cttccgaaat catgaaaacc caagatttgt cttttgcaaa 300 caaacccatt tcaaccattc ctagcaagct tttcttcggc ccaaaggacg ttgccttcac 360 cccatatggg gattactgga ggaatgccag aagcatttgc atgcttcagc ttttgaacaa 420 caaaagagtc cagtcttttc gaaagataag ggaagaagag acttctcttc ttctccagag 480 gattagggaa tcgccaaatt cagaagtcga tttaacggag ctgttcgttt ccatgactaa 540 cgacatagtt tgcagggtgg ccttaggaag gaagtattgt gatggggaag aagggaggaa 600 attcaagtct ttgctgttag agtttgtgga attgttggga gtttttaaca ttggagatta 660 catgccgtgg cttgcatgga tgaatcgttt caatggtttg aatgccaaag tggataaagt 720 ggcgaaagag tttgatgcat ttttggagga tgtgattgag gaacacggag gaaataagaa 780 atcagacact gaagctgaag gggcagactt cgtggatata ttattgcagg ttcacaaaga 840 aaacaaggct ggttttcaag tcgaaatgga tgcaatcaaa gctattatca tggatatgtt 900 tgctgcggga acagatacaa cttccacgct tctagagtgg acaatgaacg agctcttaag 960 aaatccaaaa acattgaata agttgagaga tgaggtgaga caagtgactc aagggaagac 1020 agaggtaaca gaggatgact tagagaaaat gccgtattta agagcagcag ttaaggagag 1080 ttccaggcta cactctccag tgccacttct acctcgagaa gcaattaagg atgcaaaggt 1140 tttgggctac gatatagctg cagggactca agtcctcgtt tgtccatggg caatctcaag 1200 agatccaaac ctttgggaaa atccagagga gtttcaacct gaaagattct tggatacttc 1260 catagattac aaaggcttac atttcgagtt aattccattc ggtgcaggtc ggaggggttg 1320 ccctggcatc acatttgcta agtttgtgaa tgagctagca ttggcaagat taatgttcca 1380 ttttgatttc tcgctaccaa aaggagttaa gcatgaggat ttggacgtgg aggaagctgc 1440 tggaattact gttagaagga agttccccct tttagccgtc gccactccat gctcgtgatt 1500 tttattttag agctcattct atgccttaaa aactactact agataactgc gtagtaaata 1560 atgcttggta 1570 236 494 PRT NICOTIANATABACUM 236 Met Ser Val Phe Ala Val Ile Ser Phe Phe Leu Leu Leu Phe Phe Leu 1 5 10 15 Phe Lys Ser Tyr Leu Pro Ser Ser Lys Thr Lys Lys Asn Ser Pro Pro 20 25 30 Ser Pro Ser Lys Leu Pro Leu Ile Gly His Phe His Lys Leu Gly Leu 35 40 45 Gln Pro His Arg Ser Leu Gln Lys Leu Ser Asn Glu His Gly Pro Met 50 55 60 Met Met Leu Gln Phe Gly Ser Val Pro Val Leu Ile Ala Ser Ser Ala 65 70 75 80 Glu Ala Ala Ser Glu Ile Met Lys Thr Gln Asp Leu Ser Phe Ala Asn 85 90 95 Lys Pro Ile Ser Thr Ile Pro Ser Lys Leu Phe Phe Gly Pro Lys Asp 100 105 110 Val Ala Phe Thr Pro Tyr Gly Asp Tyr Trp Arg Asn Ala Arg Ser Ile 115 120 125 Cys Met Leu Gln Leu Leu Asn Asn Lys Arg Val Gln Ser Phe Arg Lys 130 135 140 Ile Arg Glu Glu Glu Thr Ser Leu Leu Leu Gln Arg Ile Arg Glu Ser 145 150 155 160 Pro Asn Ser Glu Val Asp Leu Thr Glu Leu Phe Val Ser Met Thr Asn 165 170 175 Asp Ile Val Cys Arg Val Ala Leu Gly Arg Lys Tyr Cys Asp Gly Glu 180 185 190 Glu Gly Arg Lys Phe Lys Ser Leu Leu Leu Glu Phe Val Glu Leu Leu 195 200 205 Gly Val Phe Asn Ile Gly Asp Tyr Met Pro Trp Leu Ala Trp Met Asn 210 215 220 Arg Phe Asn Gly Leu Asn Ala Lys Val Asp Lys Val Ala Lys Glu Phe 225 230 235 240 Asp Ala Phe Leu Glu Asp Val Ile Glu Glu His Gly Gly Asn Lys Lys 245 250 255 Ser Asp Thr Glu Ala Glu Gly Ala Asp Phe Val Asp Ile Leu Leu Gln 260 265 270 Val His Lys Glu Asn Lys Ala Gly Phe Gln Val Glu Met Asp Ala Ile 275 280 285 Lys Ala Ile Ile Met Asp Met Phe Ala Ala Gly Thr Asp Thr Thr Ser 290 295 300 Thr Leu Leu Glu Trp Thr Met Asn Glu Leu Leu Arg Asn Pro Lys Thr 305 310 315 320 Leu Asn Lys Leu Arg Asp Glu Val Arg Gln Val Thr Gln Gly Lys Thr 325 330 335 Glu Val Thr Glu Asp Asp Leu Glu Lys Met Pro Tyr Leu Arg Ala Ala 340 345 350 Val Lys Glu Ser Ser Arg Leu His Ser Pro Val Pro Leu Leu Pro Arg 355 360 365 Glu Ala Ile Lys Asp Ala Lys Val Leu Gly Tyr Asp Ile Ala Ala Gly 370 375 380 Thr Gln Val Leu Val Cys Pro Trp Ala Ile Ser Arg Asp Pro Asn Leu 385 390 395 400 Trp Glu Asn Pro Glu Glu Phe Gln Pro Glu Arg Phe Leu Asp Thr Ser 405 410 415 Ile Asp Tyr Lys Gly Leu His Phe Glu Leu Ile Pro Phe Gly Ala Gly 420 425 430 Arg Arg Gly Cys Pro Gly Ile Thr Phe Ala Lys Phe Val Asn Glu Leu 435 440 445 Ala Leu Ala Arg Leu Met Phe His Phe Asp Phe Ser Leu Pro Lys Gly 450 455 460 Val Lys His Glu Asp Leu Asp Val Glu Glu Ala Ala Gly Ile Thr Val 465 470 475 480 Arg Arg Lys Phe Pro Leu Leu Ala Val Ala Thr Pro Cys Ser 485 490 237 1585 DNA NICOTIANATABACUM 237 attatccatc acctaaaatg gagaattctt gggtttttct agccttggca gggctatctg 60 cattagcttt tctctgtaaa ataatcacct gtcgaagacc ggttaaccgg aaaataccac 120 caggtccaaa accatggccc atcattggca atttgaacct acttggtcct ataccacatc 180 aatcttttga cttgctttcc aaaaaatatg gagagttgat gctgctgaaa tttggctcca 240 ggccagttct tgttgcttca tctgctgaaa tggcaaaaca gtttttaaaa gtacatgatg 300 ctaatttcgc ctcccgtcct atgctagctg gtggaaagta tacaagctat aactattgtg 360 acatgacatg ggcaccctat ggtccctatt ggcgccaagc acgacgaatt taccttaacc 420 agatatttac tccgaaaagg ctagactcgt tcgagtacat tcgtgttgaa gaaaggcagg 480 ccttgatttc ccagctgaat tcccttgctg gaaagccatt ttttctcaaa gaccatttgt 540 cgcgatttag cctctgcagc atgacaagga tggttttgag caacaagtac tttggtgaat 600 caacagttag agtagaagat ttgcagtacc tggtagatca atggttctta cttaatggtg 660 ctttcaacat tggagattgg attccatggc tcagcttctt ggacctacaa ggctatgtga 720 aacaaatgaa ggctttgaaa agaacttttg ataagttcca caacattgtg ctagatgatc 780 gcagggctaa gaagaatgca gagaagaact ttgtcccaaa agacatggtt gatgtcttgt 840 tgaagatggc tgaagatcct aatctggaag tcaaactcac taatgactgt gtcaaagggt 900 taatgcagga tttactaact ggaggaacag atagcttaac agcagcagtg caatgggcat 960 ttcaagaact tcttagacgg ccaagggtta ttgagaaggc aaccgaagag cttgaccgga 1020 ttgtcgggaa agagagatgg gtagaagaga aagattgctc gcagctatct tacgttgaag 1080 caatcctcaa ggaaacacta aggttacatc ctctaggaac tatgctagca ccgcattgtg 1140 ctatagaaga ttgtaacgtg gctggttatg acatacagaa aggaacgacc gttctggtga 1200 atgtttggac cattggaagg gacccaaaat actgggatag agcacaagag tttctccccg 1260 agagattctt agagaacgac attgatatgg acggacataa ctttgctttc ttgccatttg 1320 gctcggggcg aaggaggtgc cctggctata gccttggact taaggttatc cgagtaacat 1380 tagccaacat gttgcatgga ttcaactgga aattacctga aggtatgaag ccagaagata 1440 taagtgtgga agaacattat gggctcacta cacatcctaa gtttcctgtt cctgtgatct 1500 tggaatctag actttcttca gatctctatt cccccatcac ttaatcctaa gtgcttccta 1560 ttatagcatc atatcaatat ccctc 1585 238 508 PRT NICOTIANATABACUM 238 Met Glu Asn Ser Trp Val Phe Leu Ala Leu Ala Gly Leu Ser Ala Leu 1 5 10 15 Ala Phe Leu Cys Lys Ile Ile Thr Cys Arg Arg Pro Val Asn Arg Lys 20 25 30 Ile Pro Pro Gly Pro Lys Pro Trp Pro Ile Ile Gly Asn Leu Asn Leu 35 40 45 Leu Gly Pro Ile Pro His Gln Ser Phe Asp Leu Leu Ser Lys Lys Tyr 50 55 60 Gly Glu Leu Met Leu Leu Lys Phe Gly Ser Arg Pro Val Leu Val Ala 65 70 75 80 Ser Ser Ala Glu Met Ala Lys Gln Phe Leu Lys Val His Asp Ala Asn 85 90 95 Phe Ala Ser Arg Pro Met Leu Ala Gly Gly Lys Tyr Thr Ser Tyr Asn 100 105 110 Tyr Cys Asp Met Thr Trp Ala Pro Tyr Gly Pro Tyr Trp Arg Gln Ala 115 120 125 Arg Arg Ile Tyr Leu Asn Gln Ile Phe Thr Pro Lys Arg Leu Asp Ser 130 135 140 Phe Glu Tyr Ile Arg Val Glu Glu Arg Gln Ala Leu Ile Ser Gln Leu 145 150 155 160 Asn Ser Leu Ala Gly Lys Pro Phe Phe Leu Lys Asp His Leu Ser Arg 165 170 175 Phe Ser Leu Cys Ser Met Thr Arg Met Val Leu Ser Asn Lys Tyr Phe 180 185 190 Gly Glu Ser Thr Val Arg Val Glu Asp Leu Gln Tyr Leu Val Asp Gln 195 200 205 Trp Phe Leu Leu Asn Gly Ala Phe Asn Ile Gly Asp Trp Ile Pro Trp 210 215 220 Leu Ser Phe Leu Asp Leu Gln Gly Tyr Val Lys Gln Met Lys Ala Leu 225 230 235 240 Lys Arg Thr Phe Asp Lys Phe His Asn Ile Val Leu Asp Asp Arg Arg 245 250 255 Ala Lys Lys Asn Ala Glu Lys Asn Phe Val Pro Lys Asp Met Val Asp 260 265 270 Val Leu Leu Lys Met Ala Glu Asp Pro Asn Leu Glu Val Lys Leu Thr 275 280 285 Asn Asp Cys Val Lys Gly Leu Met Gln Asp Leu Leu Thr Gly Gly Thr 290 295 300 Asp Ser Leu Thr Ala Ala Val Gln Trp Ala Phe Gln Glu Leu Leu Arg 305 310 315 320 Arg Pro Arg Val Ile Glu Lys Ala Thr Glu Glu Leu Asp Arg Ile Val 325 330 335 Gly Lys Glu Arg Trp Val Glu Glu Lys Asp Cys Ser Gln Leu Ser Tyr 340 345 350 Val Glu Ala Ile Leu Lys Glu Thr Leu Arg Leu His Pro Leu Gly Thr 355 360 365 Met Leu Ala Pro His Cys Ala Ile Glu Asp Cys Asn Val Ala Gly Tyr 370 375 380 Asp Ile Gln Lys Gly Thr Thr Val Leu Val Asn Val Trp Thr Ile Gly 385 390 395 400 Arg Asp Pro Lys Tyr Trp Asp Arg Ala Gln Glu Phe Leu Pro Glu Arg 405 410 415 Phe Leu Glu Asn Asp Ile Asp Met Asp Gly His Asn Phe Ala Phe Leu 420 425 430 Pro Phe Gly Ser Gly Arg Arg Arg Cys Pro Gly Tyr Ser Leu Gly Leu 435 440 445 Lys Val Ile Arg Val Thr Leu Ala Asn Met Leu His Gly Phe Asn Trp 450 455 460 Lys Leu Pro Glu Gly Met Lys Pro Glu Asp Ile Ser Val Glu Glu His 465 470 475 480 Tyr Gly Leu Thr Thr His Pro Lys Phe Pro Val Pro Val Ile Leu Glu 485 490 495 Ser Arg Leu Ser Ser Asp Leu Tyr Ser Pro Ile Thr 500 505 239 1591 DNA NICOTIANATABACUM 239 ctcattatcc atcacctaaa atggagaatt cttgggtttt tctagccttg gcagggctat 60 ctgcattagc ttttctctgt aaaataatca cctgtcgaag accggttaac cggaaaatac 120 caccaggtcc aaaaccatgg cccatcattg gcaatttgaa cctacttggt cctataccac 180 atcaatcttt tgacttgctt tccaaaaaat atggagagtt gatgctgctg aaatttggct 240 ccaggccagt tcttgttgct tcatctgctg aaatggcaaa acagttttta aaagtacatg 300 atgctaattt cgcctcccgt cctatgctag ctggtggaaa gtatacaagc tataactatt 360 gtgacatgac atgggcaccc tatggtccct attggcgcca agcacgacga cgaatttacc 420 ttaaccagat atttactccg aaaaggctag actcgttcga gtacattcgt gttgaagaaa 480 ggcaggcctt gatttcccag ctgaattccc ttgctggaaa gccatttttt ctcaaagacc 540 atttgtcgcg atttagcctc tgcagcatga caaggatggt tttgagcaac aagtattttg 600 gtgaatcaac agttagagta gaagatttgc agtacctggt agatcaatgg ttcttactta 660 atggtgcttt caacattgga gattggattc catggctcag cttcttggac ctacaaggct 720 atgtgaaaca aatgaaggct ttgaaaagaa cttttgataa gttccacaac attgtgctag 780 atgatcacag ggctaagaag aatgcagaga agaactttgt cccaaaagac atggttgatg 840 tcttgttgaa gatggctgaa gatcctaatc tggaagtcaa actcactaat gactgtgtca 900 aagggttaat gcaggattta ctaactggag gaacagatag cttaacagca gcagtgcaat 960 gggcatttca agaacttctt agacagccaa gggttattga gaaggcaacc gaagagcttg 1020 accggattgt cgggaaagag agatgggtag aagagaaaga ttgctcgcag ctatcttacg 1080 ttgaagcaat cctcaaggaa acactaaggt tacatcctct aggaactatg ctagcaccgc 1140 attgtgctat agaagattgt aacgtggctg gttatgacat acagaaagga acgaccgttc 1200 tggtgaatgt ttggaccatt ggaagggacc caaaatactg ggatagagca caagagtttc 1260 tccccgagag attcttagag aacgacattg atatggacgg acataacttt gctttcttgc 1320 catttggctc ggggcgaagg aggtgccctg gctatagcct tggacttaag gttatccgag 1380 taacattagc caacatgttg catggattca actggaaatt acctgaaggt atgaagccag 1440 aagatataag tgtggaagaa cattatgggc tcactacaca tcctaagttt cctgttcctg 1500 tgatcttgga atctagactt tcttcagatc tctattcccc catcacttaa tcctaagtgc 1560 ttcctattat agcatcatat caatatccct c 1591 240 509 PRT NICOTIANATABACUM 240 Met Glu Asn Ser Trp Val Phe Leu Ala Leu Ala Gly Leu Ser Ala Leu 1 5 10 15 Ala Phe Leu Cys Lys Ile Ile Thr Cys Arg Arg Pro Val Asn Arg Lys 20 25 30 Ile Pro Pro Gly Pro Lys Pro Trp Pro Ile Ile Gly Asn Leu Asn Leu 35 40 45 Leu Gly Pro Ile Pro His Gln Ser Phe Asp Leu Leu Ser Lys Lys Tyr 50 55 60 Gly Glu Leu Met Leu Leu Lys Phe Gly Ser Arg Pro Val Leu Val Ala 65 70 75 80 Ser Ser Ala Glu Met Ala Lys Gln Phe Leu Lys Val His Asp Ala Asn 85 90 95 Phe Ala Ser Arg Pro Met Leu Ala Gly Gly Lys Tyr Thr Ser Tyr Asn 100 105 110 Tyr Cys Asp Met Thr Trp Ala Pro Tyr Gly Pro Tyr Trp Arg Gln Ala 115 120 125 Arg Arg Arg Ile Tyr Leu Asn Gln Ile Phe Thr Pro Lys Arg Leu Asp 130 135 140 Ser Phe Glu Tyr Ile Arg Val Glu Glu Arg Gln Ala Leu Ile Ser Gln 145 150 155 160 Leu Asn Ser Leu Ala Gly Lys Pro Phe Phe Leu Lys Asp His Leu Ser 165 170 175 Arg Phe Ser Leu Cys Ser Met Thr Arg Met Val Leu Ser Asn Lys Tyr 180 185 190 Phe Gly Glu Ser Thr Val Arg Val Glu Asp Leu Gln Tyr Leu Val Asp 195 200 205 Gln Trp Phe Leu Leu Asn Gly Ala Phe Asn Ile Gly Asp Trp Ile Pro 210 215 220 Trp Leu Ser Phe Leu Asp Leu Gln Gly Tyr Val Lys Gln Met Lys Ala 225 230 235 240 Leu Lys Arg Thr Phe Asp Lys Phe His Asn Ile Val Leu Asp Asp His 245 250 255 Arg Ala Lys Lys Asn Ala Glu Lys Asn Phe Val Pro Lys Asp Met Val 260 265 270 Asp Val Leu Leu Lys Met Ala Glu Asp Pro Asn Leu Glu Val Lys Leu 275 280 285 Thr Asn Asp Cys Val Lys Gly Leu Met Gln Asp Leu Leu Thr Gly Gly 290 295 300 Thr Asp Ser Leu Thr Ala Ala Val Gln Trp Ala Phe Gln Glu Leu Leu 305 310 315 320 Arg Gln Pro Arg Val Ile Glu Lys Ala Thr Glu Glu Leu Asp Arg Ile 325 330 335 Val Gly Lys Glu Arg Trp Val Glu Glu Lys Asp Cys Ser Gln Leu Ser 340 345 350 Tyr Val Glu Ala Ile Leu Lys Glu Thr Leu Arg Leu His Pro Leu Gly 355 360 365 Thr Met Leu Ala Pro His Cys Ala Ile Glu Asp Cys Asn Val Ala Gly 370 375 380 Tyr Asp Ile Gln Lys Gly Thr Thr Val Leu Val Asn Val Trp Thr Ile 385 390 395 400 Gly Arg Asp Pro Lys Tyr Trp Asp Arg Ala Gln Glu Phe Leu Pro Glu 405 410 415 Arg Phe Leu Glu Asn Asp Ile Asp Met Asp Gly His Asn Phe Ala Phe 420 425 430 Leu Pro Phe Gly Ser Gly Arg Arg Arg Cys Pro Gly Tyr Ser Leu Gly 435 440 445 Leu Lys Val Ile Arg Val Thr Leu Ala Asn Met Leu His Gly Phe Asn 450 455 460 Trp Lys Leu Pro Glu Gly Met Lys Pro Glu Asp Ile Ser Val Glu Glu 465 470 475 480 His Tyr Gly Leu Thr Thr His Pro Lys Phe Pro Val Pro Val Ile Leu 485 490 495 Glu Ser Arg Leu Ser Ser Asp Leu Tyr Ser Pro Ile Thr 500 505 241 1615 DNA NICOTIANATABACUM 241 tgataatgct ctttctactc tttgtagccc ttcctttcat tcttattttt cttcttccta 60 aattcaaaaa tggtggaaat aacagattgc caccaggtcc tataggttta ccattcattg 120 gaaatttgca tcaatacgat agtataactc ctcatatcta tttttggaaa ctttcaaaaa 180 aatatggcaa aatcttctca ttaaaacttg cttctactaa tgtggtagta gtttcttcag 240 caaaattagc aaaagaagta ttgaaaaaac aagatttaat attttgtagt agaccatcta 300 ttcttggcca acaaaaactg tcttattatg gtcgtgatat tgcttttaat gattattgga 360 gagaaatgag aaaaatttgt gttcttcatc tttttagttt aaaaaaagtt caattattta 420 gtccaattcg tgaagatgaa gtttttagaa tgattaagaa aatatcaaaa caagcttcta 480 cttcacaaat tattaatttg agtaatttaa tgatttcatt aacaagtaca attatttgta 540 gagttgcttt tggtgttagg attgaagaag aagcacatgc aaggaagaga tttgattttc 600 ttttggccga ggcacaagaa atgatggcta gtttctttgt atctgatttt tttccctttt 660 taagttggat tgataaatta agtggattga catatagact tgagaggaat ttcaaggatt 720 tggataattt ttatgaagaa ctcattgagc aacatcaaaa tcctaataag ccaaaatata 780 tggaaggaga tattgttgat cttttgctac aattgaagaa agagaaatta acaccacttg 840 atctcactat ggaagatata aaaggaattc tcatgaatgt gttagttgca ggatcagaca 900 ctagtgcagc tgctactgtt tgggcaatga cagccttgat aaagaatcct aaagccatgg 960 aaaaagttca attagaaatc agaaaatcag ttgggaagaa aggcattgta aatgaagaag 1020 atgtccaaaa catcccttat tttaaagcag tgataaagga aatatttaga ttgtatccac 1080 cagctccact tttagttcca agagaatcaa tggaaaaaac catattagaa ggttatgaaa 1140 ttcggccaag aaccatagtt catgttaacg cttgggctat agcaagggat cctgaaatat 1200 gggaaaatcc agatgaattt atacctgaga gatttttgaa tagcagtatc gattacaagg 1260 gtcaagattt tgagttactt ccatttggtg caggcagaag aggttgccca ggtattgcac 1320 ttggggttgc atccatggaa cttgctttgt caaatcttct ttatgcattt gattgggagt 1380 tgccttatgg agtaaaaaaa gaagacatcg acacaaacgt taggcctgga attgccatgc 1440 acaagaaaaa cgaactttgc cttgtcccaa aaaattattt ataaattata ttgggacgtg 1500 gatctcatgc tagttctgtg cggtcagcta agcttattat ttttggctca aattatgtat 1560 acataattag tacatgttta aaatgtataa atatagtaga accattctca tggtt 1615 242 492 PRT NICOTIANATABACUM 242 Met Leu Phe Leu Leu Phe Val Ala Leu Pro Phe Ile Leu Ile Phe Leu 1 5 10 15 Leu Pro Lys Phe Lys Asn Gly Gly Asn Asn Arg Leu Pro Pro Gly Pro 20 25 30 Ile Gly Leu Pro Phe Ile Gly Asn Leu His Gln Tyr Asp Ser Ile Thr 35 40 45 Pro His Ile Tyr Phe Trp Lys Leu Ser Lys Lys Tyr Gly Lys Ile Phe 50 55 60 Ser Leu Lys Leu Ala Ser Thr Asn Val Val Val Val Ser Ser Ala Lys 65 70 75 80 Leu Ala Lys Glu Val Leu Lys Lys Gln Asp Leu Ile Phe Cys Ser Arg 85 90 95 Pro Ser Ile Leu Gly Gln Gln Lys Leu Ser Tyr Tyr Gly Arg Asp Ile 100 105 110 Ala Phe Asn Asp Tyr Trp Arg Glu Met Arg Lys Ile Cys Val Leu His 115 120 125 Leu Phe Ser Leu Lys Lys Val Gln Leu Phe Ser Pro Ile Arg Glu Asp 130 135 140 Glu Val Phe Arg Met Ile Lys Lys Ile Ser Lys Gln Ala Ser Thr Ser 145 150 155 160 Gln Ile Ile Asn Leu Ser Asn Leu Met Ile Ser Leu Thr Ser Thr Ile 165 170 175 Ile Cys Arg Val Ala Phe Gly Val Arg Ile Glu Glu Glu Ala His Ala 180 185 190 Arg Lys Arg Phe Asp Phe Leu Leu Ala Glu Ala Gln Glu Met Met Ala 195 200 205 Ser Phe Phe Val Ser Asp Phe Phe Pro Phe Leu Ser Trp Ile Asp Lys 210 215 220 Leu Ser Gly Leu Thr Tyr Arg Leu Glu Arg Asn Phe Lys Asp Leu Asp 225 230 235 240 Asn Phe Tyr Glu Glu Leu Ile Glu Gln His Gln Asn Pro Asn Lys Pro 245 250 255 Lys Tyr Met Glu Gly Asp Ile Val Asp Leu Leu Leu Gln Leu Lys Lys 260 265 270 Glu Lys Leu Thr Pro Leu Asp Leu Thr Met Glu Asp Ile Lys Gly Ile 275 280 285 Leu Met Asn Val Leu Val Ala Gly Ser Asp Thr Ser Ala Ala Ala Thr 290 295 300 Val Trp Ala Met Thr Ala Leu Ile Lys Asn Pro Lys Ala Met Glu Lys 305 310 315 320 Val Gln Leu Glu Ile Arg Lys Ser Val Gly Lys Lys Gly Ile Val Asn 325 330 335 Glu Glu Asp Val Gln Asn Ile Pro Tyr Phe Lys Ala Val Ile Lys Glu 340 345 350 Ile Phe Arg Leu Tyr Pro Pro Ala Pro Leu Leu Val Pro Arg Glu Ser 355 360 365 Met Glu Lys Thr Ile Leu Glu Gly Tyr Glu Ile Arg Pro Arg Thr Ile 370 375 380 Val His Val Asn Ala Trp Ala Ile Ala Arg Asp Pro Glu Ile Trp Glu 385 390 395 400 Asn Pro Asp Glu Phe Ile Pro Glu Arg Phe Leu Asn Ser Ser Ile Asp 405 410 415 Tyr Lys Gly Gln Asp Phe Glu Leu Leu Pro Phe Gly Ala Gly Arg Arg 420 425 430 Gly Cys Pro Gly Ile Ala Leu Gly Val Ala Ser Met Glu Leu Ala Leu 435 440 445 Ser Asn Leu Leu Tyr Ala Phe Asp Trp Glu Leu Pro Tyr Gly Val Lys 450 455 460 Lys Glu Asp Ile Asp Thr Asn Val Arg Pro Gly Ile Ala Met His Lys 465 470 475 480 Lys Asn Glu Leu Cys Leu Val Pro Lys Asn Tyr Leu 485 490 243 1624 DNA NICOTIANATABACUM 243 tgataatgct ctttctactc tttgtagccc ttcctttcat tcttattttt cttcttccta 60 aattcaaaaa tggtggaaat aacagattgc caccaggtcc tataggttta ccattcattg 120 gaaatttgca tcaatatgat agtataactc ctcatatcta tttttggaaa ctttccaaaa 180 aatatggcaa aatcttctca ttaaaacttg cttctactaa tgtggtagta gtttcttcag 240 caaaattagc aaaagaagta ttgaaaaaac aagatttaat attttgtagt agaccatcta 300 ttcttggcca acaaaaactg tcttattatg gtcgtgatat tgcttttgca ccttataatg 360 attattggag agaaatgaga aaaatttgtg ttcttcatct ttttagttta aaaaaagttc 420 aattatttag tccaattcgt gaagatgaag tttttagaat gattaagaaa atatcaaaac 480 aagcttctac ttcacaaatt attaatttga gtaatttaat gatttcatta acaagtacaa 540 ttatttgtag agttgctttt ggtgttaggt ttgaagaaga agcacatgca aggaagagat 600 ttgattttct tttggccgag gcacaagaaa tgatggctag tttctttgta tctgattttt 660 ttcccttttt aagttggatt gataaattaa gtggattgac atatagactt gagaggaatt 720 tcaaggattt ggataatttt tatgaagaac tcattgagca acatcaaaat cctaataagc 780 caaaatatat ggaaggagat attgttgatc ttttgctaca attgaagaaa gagaaattaa 840 caccacttga tctcactatg gaagatataa aaggaattct catgaatgtg ttagttgcag 900 gatcagacac tagtgcagct gctactgttt gggcaatgac agccttgata aagaatccta 960 aagccatgga aaaagttcaa ttagaaatca gaaaatcagt tgggaagaaa ggcattgtaa 1020 atgaagaaga tgtccaaaac atcccttatt ttaaagcagt gataaaggaa atatttagat 1080 tgtatccacc agctccactt ttagttccaa gagaatcaat ggaaaaaacc atattagaag 1140 gttatgaaat tcggccaaga accatagttc atgttaacgc ttgggctata gcaagggatc 1200 ctgaaatatg ggaaaatcca gatgaattta tacctgagag atttttgaat agcagtatcg 1260 attacaaggg tcaagatttt gagttacttc catttggtgc aggcagaaga ggttgcccag 1320 gtattgcact tggggttgca tccatggaac ttgctttgtc aaatcttctt tatgcatttg 1380 attgggagtt gccttatgga gtgaaaaaag aagacatcga cacaaacgtt aggcctggaa 1440 ttgccatgca caagaaaaac gaactttgcc ttgtcccaaa aaattattta taaattatat 1500 tgggacgtgg atctcatgct agttctgtgc ggtcagctaa gcttattatt tttggctcaa 1560 attatgtata cataattagt acatgtttaa aatgtataaa tatagtagaa ccattctcat 1620 ggtt 1624 244 495 PRT NICOTIANATABACUM 244 Met Leu Phe Leu Leu Phe Val Ala Leu Pro Phe Ile Leu Ile Phe Leu 1 5 10 15 Leu Pro Lys Phe Lys Asn Gly Gly Asn Asn Arg Leu Pro Pro Gly Pro 20 25 30 Ile Gly Leu Pro Phe Ile Gly Asn Leu His Gln Tyr Asp Ser Ile Thr 35 40 45 Pro His Ile Tyr Phe Trp Lys Leu Ser Lys Lys Tyr Gly Lys Ile Phe 50 55 60 Ser Leu Lys Leu Ala Ser Thr Asn Val Val Val Val Ser Ser Ala Lys 65 70 75 80 Leu Ala Lys Glu Val Leu Lys Lys Gln Asp Leu Ile Phe Cys Ser Arg 85 90 95 Pro Ser Ile Leu Gly Gln Gln Lys Leu Ser Tyr Tyr Gly Arg Asp Ile 100 105 110 Ala Phe Ala Pro Tyr Asn Asp Tyr Trp Arg Glu Met Arg Lys Ile Cys 115 120 125 Val Leu His Leu Phe Ser Leu Lys Lys Val Gln Leu Phe Ser Pro Ile 130 135 140 Arg Glu Asp Glu Val Phe Arg Met Ile Lys Lys Ile Ser Lys Gln Ala 145 150 155 160 Ser Thr Ser Gln Ile Ile Asn Leu Ser Asn Leu Met Ile Ser Leu Thr 165 170 175 Ser Thr Ile Ile Cys Arg Val Ala Phe Gly Val Arg Phe Glu Glu Glu 180 185 190 Ala His Ala Arg Lys Arg Phe Asp Phe Leu Leu Ala Glu Ala Gln Glu 195 200 205 Met Met Ala Ser Phe Phe Val Ser Asp Phe Phe Pro Phe Leu Ser Trp 210 215 220 Ile Asp Lys Leu Ser Gly Leu Thr Tyr Arg Leu Glu Arg Asn Phe Lys 225 230 235 240 Asp Leu Asp Asn Phe Tyr Glu Glu Leu Ile Glu Gln His Gln Asn Pro 245 250 255 Asn Lys Pro Lys Tyr Met Glu Gly Asp Ile Val Asp Leu Leu Leu Gln 260 265 270 Leu Lys Lys Glu Lys Leu Thr Pro Leu Asp Leu Thr Met Glu Asp Ile 275 280 285 Lys Gly Ile Leu Met Asn Val Leu Val Ala Gly Ser Asp Thr Ser Ala 290 295 300 Ala Ala Thr Val Trp Ala Met Thr Ala Leu Ile Lys Asn Pro Lys Ala 305 310 315 320 Met Glu Lys Val Gln Leu Glu Ile Arg Lys Ser Val Gly Lys Lys Gly 325 330 335 Ile Val Asn Glu Glu Asp Val Gln Asn Ile Pro Tyr Phe Lys Ala Val 340 345 350 Ile Lys Glu Ile Phe Arg Leu Tyr Pro Pro Ala Pro Leu Leu Val Pro 355 360 365 Arg Glu Ser Met Glu Lys Thr Ile Leu Glu Gly Tyr Glu Ile Arg Pro 370 375 380 Arg Thr Ile Val His Val Asn Ala Trp Ala Ile Ala Arg Asp Pro Glu 385 390 395 400 Ile Trp Glu Asn Pro Asp Glu Phe Ile Pro Glu Arg Phe Leu Asn Ser 405 410 415 Ser Ile Asp Tyr Lys Gly Gln Asp Phe Glu Leu Leu Pro Phe Gly Ala 420 425 430 Gly Arg Arg Gly Cys Pro Gly Ile Ala Leu Gly Val Ala Ser Met Glu 435 440 445 Leu Ala Leu Ser Asn Leu Leu Tyr Ala Phe Asp Trp Glu Leu Pro Tyr 450 455 460 Gly Val Lys Lys Glu Asp Ile Asp Thr Asn Val Arg Pro Gly Ile Ala 465 470 475 480 Met His Lys Lys Asn Glu Leu Cys Leu Val Pro Lys Asn Tyr Leu 485 490 495 245 1627 DNA NICOTIANATABACUM 245 aaaattcata atggtttttc ccatagaagc ctttgtagga ctagtaacct tcacatttct 60 cttatacttc ctatggacaa aaaaatctca aaaacttcca aaacccttac taccgaaaat 120 ccccggagga tggccggtaa tcggccatct ttttcacttc aataacgacg gcgacgaccg 180 tccattagct cgaaaactcg gagacttagc tgataaatac ggccccgttt tcacttttcg 240 gctaggtctt ccccttgtgc tagttgtaag cagttacgaa gctataaaag attgcttctc 300 tacaaatgac gccattttct ccaatcgtcc agcttttctt tacggcgaat accttggcta 360 caataataca atgctttttc tagcaaatta cggaccttac tggcgaaaaa atcgtaaatt 420 agtcattcag gaagttctct ctgctagtcg tctcgaaaaa ttcaaacaag tgagattcac 480 cagaattcaa acgagcatta agaatttata cactcgaatt aatggaaatt cgagtacgat 540 aaatctaact gattggttag aagaattgga ttttggtctg atcgtgaaaa tgatcgctgg 600 gaaaaattat gaatccggta aaggagatga acaagtggaa agatttaaga atgcgtttaa 660 ggattttatg gttttatcaa tggaatttgt attatgggat gcatttccaa ttccattatt 720 taaatgggtg gattttcaag gtcatattaa ggcaatgaaa aggacattta aggatataga 780 ttctgttttt cagaactggt tagaggaaca tattaataaa agagaaaaaa tggaggttgg 840 tgcagaaggg aatgaacaag atttcattga tgtggtgctt tcaaaattga gtaaagaata 900 tcttgatgaa ggttactctc gtgatactgt cattaaagca acagttttta gtttggtctt 960 ggatgcagca gacacagttg ctcttcacat aaattgggga atgacattat tgataaacaa 1020 tcaaaatgcc ttgatgaaag cacaagaaga gatagacaca aaagttggta agtatagatg 1080 ggtagaagag agtgatatta aggatttagt atacctccaa gctattgtta aaaaggtgtt 1140 acgattatat ccaccaggac ctttgttagt accacatgaa tatgtaaagg attgtgttgt 1200 tagtggatat cacattccta aagggactag attattcgca aacgtcatga aactgcagcg 1260 cgatcctaaa ctcttgtcaa atcctgataa gttcgatcca gagagattca tcgctggtga 1320 tatcgacttc cgtggtcacc actatgagtt tatcccattt ggttctggaa gacgatcttg 1380 tccggggatg acttatgcat tgcaagtgga acacctaaca atggcacatt taatccaggg 1440 tttcaattac aaaactccaa atgacgaggc cttggatatg aaggaaggtg caggcataac 1500 aatacgtaag gtaaatccgg tggaattgat aataacgcct cgcttggcac ctgagcttta 1560 ctaaaaccta agatctttca tcttggttga tcattgttta atactcctag atagatgggt 1620 attcatc 1627 246 517 PRT NICOTIANATABACUM 246 Met Val Phe Pro Ile Glu Ala Phe Val Gly Leu Val Thr Phe Thr Phe 1 5 10 15 Leu Leu Tyr Phe Leu Trp Thr Lys Lys Ser Gln Lys Leu Pro Lys Pro 20 25 30 Leu Leu Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly His Leu Phe 35 40 45 His Phe Asn Asn Asp Gly Asp Asp Arg Pro Leu Ala Arg Lys Leu Gly 50 55 60 Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe Arg Leu Gly Leu 65 70 75 80 Pro Leu Val Leu Val Val Ser Ser Tyr Glu Ala Ile Lys Asp Cys Phe 85 90 95 Ser Thr Asn Asp Ala Ile Phe Ser Asn Arg Pro Ala Phe Leu Tyr Gly 100 105 110 Glu Tyr Leu Gly Tyr Asn Asn Thr Met Leu Phe Leu Ala Asn Tyr Gly 115 120 125 Pro Tyr Trp Arg Lys Asn Arg Lys Leu Val Ile Gln Glu Val Leu Ser 130 135 140 Ala Ser Arg Leu Glu Lys Phe Lys Gln Val Arg Phe Thr Arg Ile Gln 145 150 155 160 Thr Ser Ile Lys Asn Leu Tyr Thr Arg Ile Asn Gly Asn Ser Ser Thr 165 170 175 Ile Asn Leu Thr Asp Trp Leu Glu Glu Leu Asp Phe Gly Leu Ile Val 180 185 190 Lys Met Ile Ala Gly Lys Asn Tyr Glu Ser Gly Lys Gly Asp Glu Gln 195 200 205 Val Glu Arg Phe Lys Asn Ala Phe Lys Asp Phe Met Val Leu Ser Met 210 215 220 Glu Phe Val Leu Trp Asp Ala Phe Pro Ile Pro Leu Phe Lys Trp Val 225 230 235 240 Asp Phe Gln Gly His Ile Lys Ala Met Lys Arg Thr Phe Lys Asp Ile 245 250 255 Asp Ser Val Phe Gln Asn Trp Leu Glu Glu His Ile Asn Lys Arg Glu 260 265 270 Lys Met Glu Val Gly Ala Glu Gly Asn Glu Gln Asp Phe Ile Asp Val 275 280 285 Val Leu Ser Lys Leu Ser Lys Glu Tyr Leu Asp Glu Gly Tyr Ser Arg 290 295 300 Asp Thr Val Ile Lys Ala Thr Val Phe Ser Leu Val Leu Asp Ala Ala 305 310 315 320 Asp Thr Val Ala Leu His Ile Asn Trp Gly Met Thr Leu Leu Ile Asn 325 330 335 Asn Gln Asn Ala Leu Met Lys Ala Gln Glu Glu Ile Asp Thr Lys Val 340 345 350 Gly Lys Tyr Arg Trp Val Glu Glu Ser Asp Ile Lys Asp Leu Val Tyr 355 360 365 Leu Gln Ala Ile Val Lys Lys Val Leu Arg Leu Tyr Pro Pro Gly Pro 370 375 380 Leu Leu Val Pro His Glu Tyr Val Lys Asp Cys Val Val Ser Gly Tyr 385 390 395 400 His Ile Pro Lys Gly Thr Arg Leu Phe Ala Asn Val Met Lys Leu Gln 405 410 415 Arg Asp Pro Lys Leu Leu Ser Asn Pro Asp Lys Phe Asp Pro Glu Arg 420 425 430 Phe Ile Ala Gly Asp Ile Asp Phe Arg Gly His His Tyr Glu Phe Ile 435 440 445 Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Met Thr Tyr Ala Leu 450 455 460 Gln Val Glu His Leu Thr Met Ala His Leu Ile Gln Gly Phe Asn Tyr 465 470 475 480 Lys Thr Pro Asn Asp Glu Ala Leu Asp Met Lys Glu Gly Ala Gly Ile 485 490 495 Thr Ile Arg Lys Val Asn Pro Val Glu Leu Ile Ile Thr Pro Arg Leu 500 505 510 Ala Pro Glu Leu Tyr 515 247 1594 DNA NICOTIANATABACUM 247 caaaaaatca tttctctcgt ctaaaatgga tcttctctta ctagagaaga ccttaattgg 60 tcttttcttt gccattttaa tcgctttaat tgtctctaaa cttcgttcaa agcgttttaa 120 gcttcctcca ggaccaattc cagtaccagt ttttggtaat tggcttcaag ttggtgatga 180 tttaaaccac agaaatctta ctgattatgc caaaaaattt ggcgatcttt tcttgttaag 240 aatgggtcaa cgtaacttag ttgttgtgtc atctcctgaa ttagctaaag aagttttaca 300 cacacaaggt gttgaatttg gttcaagaac aagaaatgtt gtgtttgata tttttactgg 360 aaaaggtcaa gatatggttt ttactgtata tggtgaacat tggagaaaaa tgaggagaat 420 tatgactgta ccatttttta ctaataaagt tgtgcaacag tatagagggg ggtgggagtt 480 tgaggtggca agtgtaattg aggatgtgaa aaaaaatcct gaatctgcta ctaatgggat 540 cgtattaagg aggagattac aattaatgat gtataataat atgtttagga ttatgtttga 600 taggagattt gagagtgaag atgatccttt gtttgttaag cttaaggctt tgaatggtga 660 aaggagtaga ttggctcaaa gttttgagta taattatggt gattttattc caattttgag 720 gcctcttttg agaggttatt tgaagatctg taaagaagtt aaggagaaga ggctgcagct 780 tttcaaagat tactttgttg atgaaagaaa gaagctttca aataccaaga gctcggacag 840 caatgcccta aaatgtgcga ttgatcacat tcttgaggct caacagaagg gagagatcaa 900 tgaggacaac gttctttaca ttgttgaaaa catcaatgtt gctgcaattg aaacaacatt 960 atggtcaatt gagtggggta tcgccgagct agtcaaccac cctcacatcc aaaagaaact 1020 gcgcgacgag attgacacag ttcttggacc aggagtgcaa gtgactgaac cagacaccca 1080 caagcttcca taccttcagg ctgtgatcaa ggaggcactt cgtctccgta tggcaattcc 1140 tctattagtc ccacacatga accttcacga cgcaaagctt ggcgggcttg atattccagc 1200 agagagcaaa atcttggtta acgcttggtg gttagctaac aacccggctc attggaagaa 1260 acccgaagag ttcagacccg agaggttctt tgaagaggag aagcatgttg aggccaatgg 1320 caatgacttc agatatcttc cgtttggcgt tggtaggagg agctgccctg gaattatact 1380 tgcattgcca attcttggca tcactttggg acgtttggtt cagaactttg agctgttgcc 1440 tcctccaggc cagtcgaagc tcgacaccac agagaaaggt ggacagttca gtctccacat 1500 tttgaagcat tccaccattg tgttgaaacc aaggtctttc tgaactttgt gatcttatta 1560 attaaggggt tctgaagaaa tttgatagtg ttgg 1594 248 505 PRT NICOTIANATABACUM 248 Met Asp Leu Leu Leu Leu Glu Lys Thr Leu Ile Gly Leu Phe Phe Ala 1 5 10 15 Ile Leu Ile Ala Leu Ile Val Ser Lys Leu Arg Ser Lys Arg Phe Lys 20 25 30 Leu Pro Pro Gly Pro Ile Pro Val Pro Val Phe Gly Asn Trp Leu Gln 35 40 45 Val Gly Asp Asp Leu Asn His Arg Asn Leu Thr Asp Tyr Ala Lys Lys 50 55 60 Phe Gly Asp Leu Phe Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val 65 70 75 80 Val Ser Ser Pro Glu Leu Ala Lys Glu Val Leu His Thr Gln Gly Val 85 90 95 Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly 100 105 110 Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp Arg Lys 115 120 125 Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln 130 135 140 Gln Tyr Arg Gly Gly Trp Glu Phe Glu Val Ala Ser Val Ile Glu Asp 145 150 155 160 Val Lys Lys Asn Pro Glu Ser Ala Thr Asn Gly Ile Val Leu Arg Arg 165 170 175 Arg Leu Gln Leu Met Met Tyr Asn Asn Met Phe Arg Ile Met Phe Asp 180 185 190 Arg Arg Phe Glu Ser Glu Asp Asp Pro Leu Phe Val Lys Leu Lys Ala 195 200 205 Leu Asn Gly Glu Arg Ser Arg Leu Ala Gln Ser Phe Glu Tyr Asn Tyr 210 215 220 Gly Asp Phe Ile Pro Ile Leu Arg Pro Leu Leu Arg Gly Tyr Leu Lys 225 230 235 240 Ile Cys Lys Glu Val Lys Glu Lys Arg Leu Gln Leu Phe Lys Asp Tyr 245 250 255 Phe Val Asp Glu Arg Lys Lys Leu Ser Asn Thr Lys Ser Ser Asp Ser 260 265 270 Asn Ala Leu Lys Cys Ala Ile Asp His Ile Leu Glu Ala Gln Gln Lys 275 280 285 Gly Glu Ile Asn Glu Asp Asn Val Leu Tyr Ile Val Glu Asn Ile Asn 290 295 300 Val Ala Ala Ile Glu Thr Thr Leu Trp Ser Ile Glu Trp Gly Ile Ala 305 310 315 320 Glu Leu Val Asn His Pro His Ile Gln Lys Lys Leu Arg Asp Glu Ile 325 330 335 Asp Thr Val Leu Gly Pro Gly Val Gln Val Thr Glu Pro Asp Thr His 340 345 350 Lys Leu Pro Tyr Leu Gln Ala Val Ile Lys Glu Ala Leu Arg Leu Arg 355 360 365 Met Ala Ile Pro Leu Leu Val Pro His Met Asn Leu His Asp Ala Lys 370 375 380 Leu Gly Gly Leu Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn Ala 385 390 395 400 Trp Trp Leu Ala Asn Asn Pro Ala His Trp Lys Lys Pro Glu Glu Phe 405 410 415 Arg Pro Glu Arg Phe Phe Glu Glu Glu Lys His Val Glu Ala Asn Gly 420 425 430 Asn Asp Phe Arg Tyr Leu Pro Phe Gly Val Gly Arg Arg Ser Cys Pro 435 440 445 Gly Ile Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Leu Gly Arg Leu 450 455 460 Val Gln Asn Phe Glu Leu Leu Pro Pro Pro Gly Gln Ser Lys Leu Asp 465 470 475 480 Thr Thr Glu Lys Gly Gly Gln Phe Ser Leu His Ile Leu Lys His Ser 485 490 495 Thr Ile Val Leu Lys Pro Arg Ser Phe 500 505 249 1678 DNA NICOTIANATABACUM 249 aacattttgc aatatagttt tcctagtcag ttctagcctc cttttcctta gaaataatgg 60 attatcatat ttctttccat tttcaagctc ttttagggct tttagccttt gtgttcttgt 120 ctattatctt atggagaaga acactcactt caagaaaatt agcccctgaa atcccagggg 180 catggcctat tataggccat cttcgtcagc tgagtggtac tgataagaat atcccatttc 240 cccgaatatt gggcgctttg gcagataaat atggacctgt cttcacactg agaataggga 300 tgtaccccta tttgattgtc aacaattggg aagcagctaa ggattgtctc acaacgcatg 360 ataaggactt cgctgcccga ccaacttcta tggctggtga aagcatcggg tacaagtatg 420 cgaggtttac ttatgctaat tttggtcctt attataacca agtgcgcaaa ctagccctac 480 aacatgtacc ctcgagtact aaactcgaga aaatgaaaca catacgtgtt tctgaattgg 540 aaactagcat caaagaatta tattctttga cgctgggcaa aaacaacatg caaaaagtga 600 atataagtaa atggtttgaa caattgactt taaacataat cgtgaagaca atttgtggca 660 agagatatag caacatagag gaggatgaag aggcacaacg tttcagaaag gcatttaagg 720 gcatcatgtt tgttgtaggg caaattgttt tatatgacgc aattccattc ccattgttca 780 aatactttga tttccaaggt catatacaat tgatgaacaa aatttataaa gacttagatt 840 ctattcttca aggatggttg gatgatcata tgatgaacaa ggatgtaaac aataaggatc 900 aagatgccat agatgccatg cttaaggtaa cacaacttaa tgaattcaaa gcctatggtt 960 tttctcaggc cactgtgatc aagtcgacag tcttgagttt gatcttagat ggaaatgaca 1020 caaccgctgt tcatttgata tgggtaatgt ccttattact gaacaatcca catgttatga 1080 aacaaggcca agaagagata gacatgaaag tgggtaaaga gaggtggatt gaagatactg 1140 acataaaaaa tttagtgtac cttcaggcta tcgttaaaga gacattgcgc ttgtatccac 1200 ctgttccttt tcttttacca cacgaagcag tgcaagattg taaagtgact ggttaccaca 1260 ttcctaaagg tactcgtcta tatatcaatg cgtggaaagt acatcgcgat cctgaaattt 1320 ggtcagagcc cgaaaagttt atgcccaata gattcttgac tagcaaagca aatatagatg 1380 ctcgcggtca aaattttgaa tttataccgt ttggttctgg gagacggtca tgtccaggga 1440 taggttttgc gactttagtg acacatctga cttttggtcg cttgcttcaa ggttttgatt 1500 ttagtaagcc atcaaacacg ccaattgaca tgacagaagg cgtaggcgtt actttgccta 1560 aggttaatca agttgaagtt ctaattaccc ctcgtttacc ttctaagctt tatttatttt 1620 gaaagtgcaa atcatcaatc atggcttgag taattagtta tactttaata tgtttctc 1678 250 521 PRT NICOTIANATABACUM 250 Met Asp Tyr His Ile Ser Phe His Phe Gln Ala Leu Leu Gly Leu Leu 1 5 10 15 Ala Phe Val Phe Leu Ser Ile Ile Leu Trp Arg Arg Thr Leu Thr Ser 20 25 30 Arg Lys Leu Ala Pro Glu Ile Pro Gly Ala Trp Pro Ile Ile Gly His 35 40 45 Leu Arg Gln Leu Ser Gly Thr Asp Lys Asn Ile Pro Phe Pro Arg Ile 50 55 60 Leu Gly Ala Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Leu Arg Ile 65 70 75 80 Gly Met Tyr Pro Tyr Leu Ile Val Asn Asn Trp Glu Ala Ala Lys Asp 85 90 95 Cys Leu Thr Thr His Asp Lys Asp Phe Ala Ala Arg Pro Thr Ser Met 100 105 110 Ala Gly Glu Ser Ile Gly Tyr Lys Tyr Ala Arg Phe Thr Tyr Ala Asn 115 120 125 Phe Gly Pro Tyr Tyr Asn Gln Val Arg Lys Leu Ala Leu Gln His Val 130 135 140 Pro Ser Ser Thr Lys Leu Glu Lys Met Lys His Ile Arg Val Ser Glu 145 150 155 160 Leu Glu Thr Ser Ile Lys Glu Leu Tyr Ser Leu Thr Leu Gly Lys Asn 165 170 175 Asn Met Gln Lys Val Asn Ile Ser Lys Trp Phe Glu Gln Leu Thr Leu 180 185 190 Asn Ile Ile Val Lys Thr Ile Cys Gly Lys Arg Tyr Ser Asn Ile Glu 195 200 205 Glu Asp Glu Glu Ala Gln Arg Phe Arg Lys Ala Phe Lys Gly Ile Met 210 215 220 Phe Val Val Gly Gln Ile Val Leu Tyr Asp Ala Ile Pro Phe Pro Leu 225 230 235 240 Phe Lys Tyr Phe Asp Phe Gln Gly His Ile Gln Leu Met Asn Lys Ile 245 250 255 Tyr Lys Asp Leu Asp Ser Ile Leu Gln Gly Trp Leu Asp Asp His Met 260 265 270 Met Asn Lys Asp Val Asn Asn Lys Asp Gln Asp Ala Ile Asp Ala Met 275 280 285 Leu Lys Val Thr Gln Leu Asn Glu Phe Lys Ala Tyr Gly Phe Ser Gln 290 295 300 Ala Thr Val Ile Lys Ser Thr Val Leu Ser Leu Ile Leu Asp Gly Asn 305 310 315 320 Asp Thr Thr Ala Val His Leu Ile Trp Val Met Ser Leu Leu Leu Asn 325 330 335 Asn Pro His Val Met Lys Gln Gly Gln Glu Glu Ile Asp Met Lys Val 340 345 350 Gly Lys Glu Arg Trp Ile Glu Asp Thr Asp Ile Lys Asn Leu Val Tyr 355 360 365 Leu Gln Ala Ile Val Lys Glu Thr Leu Arg Leu Tyr Pro Pro Val Pro 370 375 380 Phe Leu Leu Pro His Glu Ala Val Gln Asp Cys Lys Val Thr Gly Tyr 385 390 395 400 His Ile Pro Lys Gly Thr Arg Leu Tyr Ile Asn Ala Trp Lys Val His 405 410 415 Arg Asp Pro Glu Ile Trp Ser Glu Pro Glu Lys Phe Met Pro Asn Arg 420 425 430 Phe Leu Thr Ser Lys Ala Asn Ile Asp Ala Arg Gly Gln Asn Phe Glu 435 440 445 Phe Ile Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Ile Gly Phe 450 455 460 Ala Thr Leu Val Thr His Leu Thr Phe Gly Arg Leu Leu Gln Gly Phe 465 470 475 480 Asp Phe Ser Lys Pro Ser Asn Thr Pro Ile Asp Met Thr Glu Gly Val 485 490 495 Gly Val Thr Leu Pro Lys Val Asn Gln Val Glu Val Leu Ile Thr Pro 500 505 510 Arg Leu Pro Ser Lys Leu Tyr Leu Phe 515 520 251 1537 DNA NICOTIANATABACUM 251 tgataatgct ctttctactc tttgtagccc ttcctttcat tcttattttt cttcttccta 60 aattcaaaaa tggtggaaat aacagattgc caccaggtcc tataggttta ccattcattg 120 gaaatttgca tcaatatgat agtataactc ctcatatcta tttttggaaa ctttccaaaa 180 aatatggcaa aatcttctca ttaaaacttg cttctactaa tgtggtagta gtttcttcag 240 caaaattagc aaaagaagta ttgaaaaaac aagatttaat attttgtagt agaccatcta 300 ttcttggcca acaaaaactg tcttattatg gtcgtgatat tgcttttgca ccttataatg 360 attattggag agaaatgaga aaaatttgtg ttcttcatct ttttagttta aaaaaagttc 420 aattatttag tccaattcgt gaagatgaag tttttagaat gattaagaaa atatcaaaac 480 aagcttctac ttcacaaatt attaatttga gtaatttaat gatttcatta acaagtacaa 540 ttatttgtag agttgctttt ggtgttaggt ttgaagaaga agcacatgca aggaagagat 600 ttgattttct tttggccgag gcacaagaaa tgatggctag tttctttgta tctgattttt 660 ttcccttttt aagttggatt gataaattaa gtggattgac atatagactt gagaggaatt 720 tcaaggattt ggataatttt tatgaagaac tcattgagca acatcaaaat cctaataagc 780 caaaatatat ggaaggagat attgttgatc ttttgctaca attgaagaaa gagaaattaa 840 caccacttga tctcactatg gaagatataa aaggaattct catgaatgtg ttagttgcag 900 gatcagacac tagtgcagct gctactgttt gggcaatgac agccttgata aagaatccta 960 aagccatgga aaaagttcaa ttagaaatca gaaaatcagt tgggaagaaa ggcattgtaa 1020 atgaagaaga tgtccaaaac atcccttatt ttaaagcagt gataaaggaa atatttagat 1080 tgtatccacc agctccactt ttagttccaa gagaatcaat ggaaaaaacc atattagaag 1140 gttatgaaat tcggccaaga accatagttc atgttaacgc ttgggctata gcaagggatc 1200 ctgaaatatg ggaaaatcca gatgaattta tacctgagag atttttgaat agcagtaccg 1260 attacaaggg tcaagatttt gagttacttc catttggtgc aggcagaaga ggttgcccag 1320 gtattgcact tggggttgca tccatggaac ttgctttgtc aaatcttctt tatgcatttg 1380 attgggagtt gccttatgga gtgaaaaaag aagacatcga cacaaacgtt aggcctggaa 1440 ttgccatgca caagaaaaac gaactttgcc ttgtcccaaa aaattattta taaattatat 1500 tgggacgtgg atctcaattt agttctgtga ggtcagc 1537 252 495 PRT NICOTIANATABACUM 252 Met Leu Phe Leu Leu Phe Val Ala Leu Pro Phe Ile Leu Ile Phe Leu 1 5 10 15 Leu Pro Lys Phe Lys Asn Gly Gly Asn Asn Arg Leu Pro Pro Gly Pro 20 25 30 Ile Gly Leu Pro Phe Ile Gly Asn Leu His Gln Tyr Asp Ser Ile Thr 35 40 45 Pro His Ile Tyr Phe Trp Lys Leu Ser Lys Lys Tyr Gly Lys Ile Phe 50 55 60 Ser Leu Lys Leu Ala Ser Thr Asn Val Val Val Val Ser Ser Ala Lys 65 70 75 80 Leu Ala Lys Glu Val Leu Lys Lys Gln Asp Leu Ile Phe Cys Ser Arg 85 90 95 Pro Ser Ile Leu Gly Gln Gln Lys Leu Ser Tyr Tyr Gly Arg Asp Ile 100 105 110 Ala Phe Ala Pro Tyr Asn Asp Tyr Trp Arg Glu Met Arg Lys Ile Cys 115 120 125 Val Leu His Leu Phe Ser Leu Lys Lys Val Gln Leu Phe Ser Pro Ile 130 135 140 Arg Glu Asp Glu Val Phe Arg Met Ile Lys Lys Ile Ser Lys Gln Ala 145 150 155 160 Ser Thr Ser Gln Ile Ile Asn Leu Ser Asn Leu Met Ile Ser Leu Thr 165 170 175 Ser Thr Ile Ile Cys Arg Val Ala Phe Gly Val Arg Phe Glu Glu Glu 180 185 190 Ala His Ala Arg Lys Arg Phe Asp Phe Leu Leu Ala Glu Ala Gln Glu 195 200 205 Met Met Ala Ser Phe Phe Val Ser Asp Phe Phe Pro Phe Leu Ser Trp 210 215 220 Ile Asp Lys Leu Ser Gly Leu Thr Tyr Arg Leu Glu Arg Asn Phe Lys 225 230 235 240 Asp Leu Asp Asn Phe Tyr Glu Glu Leu Ile Glu Gln His Gln Asn Pro 245 250 255 Asn Lys Pro Lys Tyr Met Glu Gly Asp Ile Val Asp Leu Leu Leu Gln 260 265 270 Leu Lys Lys Glu Lys Leu Thr Pro Leu Asp Leu Thr Met Glu Asp Ile 275 280 285 Lys Gly Ile Leu Met Asn Val Leu Val Ala Gly Ser Asp Thr Ser Ala 290 295 300 Ala Ala Thr Val Trp Ala Met Thr Ala Leu Ile Lys Asn Pro Lys Ala 305 310 315 320 Met Glu Lys Val Gln Leu Glu Ile Arg Lys Ser Val Gly Lys Lys Gly 325 330 335 Ile Val Asn Glu Glu Asp Val Gln Asn Ile Pro Tyr Phe Lys Ala Val 340 345 350 Ile Lys Glu Ile Phe Arg Leu Tyr Pro Pro Ala Pro Leu Leu Val Pro 355 360 365 Arg Glu Ser Met Glu Lys Thr Ile Leu Glu Gly Tyr Glu Ile Arg Pro 370 375 380 Arg Thr Ile Val His Val Asn Ala Trp Ala Ile Ala Arg Asp Pro Glu 385 390 395 400 Ile Trp Glu Asn Pro Asp Glu Phe Ile Pro Glu Arg Phe Leu Asn Ser 405 410 415 Ser Thr Asp Tyr Lys Gly Gln Asp Phe Glu Leu Leu Pro Phe Gly Ala 420 425 430 Gly Arg Arg Gly Cys Pro Gly Ile Ala Leu Gly Val Ala Ser Met Glu 435 440 445 Leu Ala Leu Ser Asn Leu Leu Tyr Ala Phe Asp Trp Glu Leu Pro Tyr 450 455 460 Gly Val Lys Lys Glu Asp Ile Asp Thr Asn Val Arg Pro Gly Ile Ala 465 470 475 480 Met His Lys Lys Asn Glu Leu Cys Leu Val Pro Lys Asn Tyr Leu 485 490 495 253 1572 DNA NICOTIANATABACUM 253 ccaaaatcat ggctctatct ttcatattca tatccataac cctaattttt ctagttcata 60 aactctacca ccgtcttaga ttcaaactac caccaggtcc gcggccgtta ccggtggtcg 120 gaaacctcta cgacataaaa ccggtgagat tccggtgctt tgccgattgg gccaaaactt 180 acggtccgat tttctcagta tactttgggt cacagttaaa tgttgtggta acaacagctg 240 aattagctaa agaagtattg aaagaaaatg accagaattt agcagataga tttaggacta 300 gacctgcaaa taatttgagc agaaatggga tggatttgat ttgggctgat tatgggcctc 360 attatgtgaa agtaaggaag ctctgtaatc ttgagctttt tactcctaaa agacttgaag 420 ctcttagacc tattagagaa gatgaagtta ctgctatggt tgaaaacatt ttcaaggatt 480 gtactaagcc tgataacaca ggtaaaagct tgttgataag agagtactta ggatcagtag 540 cattcaacaa cattacaagg ttaacatttg ggaaaaggtt catgaactca aaaggtgaga 600 ttgatgagca aggtcaagaa ttcaagggta ttgtctctaa tggcatcaaa attggcggaa 660 aacttccctt ggcagagtat gttccatggc tccgttggtt tttcacaatg gaaaacgagg 720 cactcgtgaa gcactctgca cgtagagacc ggttaacaag aatgatcatg gatgaacaca 780 cactggctcg caagaaaact ggtgatacta agcagcattt tgtcgatgca ttgcttactc 840 ttcagaagca gtatgatctt agtgatgaca ctgttattgg cctcctctgg gatatgatta 900 cagcaggaat ggacacaaca accataacag tggaatgggc aatggcagaa ctagttaaga 960 acccaagagt gcaactaaaa gctcaagagg agcttgacag ggtaatcgga acggatcgaa 1020 tcatgtcaga aaccgatttc tctaaacttc cttacctaca atgtgtagcc aaagaggctc 1080 taaggttgca ccctccaact cctctaatgc ttcctcataa ggccagtgcc agtgtcaaaa 1140 ttggtggtta tgacattcct aaggggtcca tcgtgcacgt gaacgtttgg gctgtcgctc 1200 gtgacccagc cgtgtggaag aacccgttgg agttcagacc agagcgcttc cttgaggaag 1260 acgttgacat gaagggtcac gactatcggt tattgccctt tggtgcagga aggcgtgttt 1320 gccccggtgc acaacttgct atcaacttgg tcacatctat gttgggtcat ttgttgcatc 1380 attttacatg ggctccggcc ccgggggtta acccggagga tattgacttg gaggagagcc 1440 ctggaacagt aacttacatg aaaaatccaa tacaagctat tccaactcca agattgcctg 1500 cacacttgta tggacgtgtg ccagtggata tgtaaaacat tttgttcttt ccctttttgg 1560 ttatatgatg ag 1572 254 508 PRT NICOTIANATABACUM 254 Met Ala Leu Ser Phe Ile Phe Ile Ser Ile Thr Leu Ile Phe Leu Val 1 5 10 15 His Lys Leu Tyr His Arg Leu Arg Phe Lys Leu Pro Pro Gly Pro Arg 20 25 30 Pro Leu Pro Val Val Gly Asn Leu Tyr Asp Ile Lys Pro Val Arg Phe 35 40 45 Arg Cys Phe Ala Asp Trp Ala Lys Thr Tyr Gly Pro Ile Phe Ser Val 50 55 60 Tyr Phe Gly Ser Gln Leu Asn Val Val Val Thr Thr Ala Glu Leu Ala 65 70 75 80 Lys Glu Val Leu Lys Glu Asn Asp Gln Asn Leu Ala Asp Arg Phe Arg 85 90 95 Thr Arg Pro Ala Asn Asn Leu Ser Arg Asn Gly Met Asp Leu Ile Trp 100 105 110 Ala Asp Tyr Gly Pro His Tyr Val Lys Val Arg Lys Leu Cys Asn Leu 115 120 125 Glu Leu Phe Thr Pro Lys Arg Leu Glu Ala Leu Arg Pro Ile Arg Glu 130 135 140 Asp Glu Val Thr Ala Met Val Glu Asn Ile Phe Lys Asp Cys Thr Lys 145 150 155 160 Pro Asp Asn Thr Gly Lys Ser Leu Leu Ile Arg Glu Tyr Leu Gly Ser 165 170 175 Val Ala Phe Asn Asn Ile Thr Arg Leu Thr Phe Gly Lys Arg Phe Met 180 185 190 Asn Ser Lys Gly Glu Ile Asp Glu Gln Gly Gln Glu Phe Lys Gly Ile 195 200 205 Val Ser Asn Gly Ile Lys Ile Gly Gly Lys Leu Pro Leu Ala Glu Tyr 210 215 220 Val Pro Trp Leu Arg Trp Phe Phe Thr Met Glu Asn Glu Ala Leu Val 225 230 235 240 Lys His Ser Ala Arg Arg Asp Arg Leu Thr Arg Met Ile Met Asp Glu 245 250 255 His Thr Leu Ala Arg Lys Lys Thr Gly Asp Thr Lys Gln His Phe Val 260 265 270 Asp Ala Leu Leu Thr Leu Gln Lys Gln Tyr Asp Leu Ser Asp Asp Thr 275 280 285 Val Ile Gly Leu Leu Trp Asp Met Ile Thr Ala Gly Met Asp Thr Thr 290 295 300 Thr Ile Thr Val Glu Trp Ala Met Ala Glu Leu Val Lys Asn Pro Arg 305 310 315 320 Val Gln Leu Lys Ala Gln Glu Glu Leu Asp Arg Val Ile Gly Thr Asp 325 330 335 Arg Ile Met Ser Glu Thr Asp Phe Ser Lys Leu Pro Tyr Leu Gln Cys 340 345 350 Val Ala Lys Glu Ala Leu Arg Leu His Pro Pro Thr Pro Leu Met Leu 355 360 365 Pro His Lys Ala Ser Ala Ser Val Lys Ile Gly Gly Tyr Asp Ile Pro 370 375 380 Lys Gly Ser Ile Val His Val Asn Val Trp Ala Val Ala Arg Asp Pro 385 390 395 400 Ala Val Trp Lys Asn Pro Leu Glu Phe Arg Pro Glu Arg Phe Leu Glu 405 410 415 Glu Asp Val Asp Met Lys Gly His Asp Tyr Arg Leu Leu Pro Phe Gly 420 425 430 Ala Gly Arg Arg Val Cys Pro Gly Ala Gln Leu Ala Ile Asn Leu Val 435 440 445 Thr Ser Met Leu Gly His Leu Leu His His Phe Thr Trp Ala Pro Ala 450 455 460 Pro Gly Val Asn Pro Glu Asp Ile Asp Leu Glu Glu Ser Pro Gly Thr 465 470 475 480 Val Thr Tyr Met Lys Asn Pro Ile Gln Ala Ile Pro Thr Pro Arg Leu 485 490 495 Pro Ala His Leu Tyr Gly Arg Val Pro Val Asp Met 500 505 255 1580 DNA NICOTIANATABACUM 255 aatcactaat tttcatgtac tctcataggt caaaagtttc aaccaaaatc atggctctat 60 ccttcatatt catatccata accctaattt ttctagttca taaactctac caccgtctta 120 gattcaaact accaccaggt ccgcggccgt taccggtggt cggaaacctc tacgacatag 180 aaccggtgag attccggtgc tttgccgatt gggccaaaac ttacggtccg attttctcag 240 tatactttgg gtcacagtta aatgttgtgg taacaacagc tgaattagct aaagaagtat 300 tgaaagaaaa tgaccagaat ttagcagata gatttaggac tagacctgca aataatttga 360 gcagaaatgg gatggatttg atttgggctg attatgggcc tcattatgtg aaagtaagga 420 agctctgtaa tcttgagctt tttactccta aaagacttga agctcttaga cctattagag 480 aagatgaagt tactgctatg gttgaaaaca ttttcaagga ttgtactaag cctgataaca 540 caggtaaaag cttgttgata agagagtact taggatcagt agcattcaac aacattacaa 600 ggttaacatt tgggaaaagg ttcatgaact caaaaggtga gattgatgag caaggtcaag 660 aattcaaggg tattgtctct aatggcatca aaattggcgg aaaacttccc ttggcagagt 720 atgttccatg gctccgttgg tttttcacaa tggaaaacga ggcactcgtg aagcactctg 780 cacgtagaga ccggttaaca agaatgatca tggatgaaca cacactggct cgcaagaaaa 840 ctggtgatac taagcagcat tttgtcgatg cattgcttac tcttcagaag cagtatgatc 900 ttagtgatga cactgttatt ggcctcctct gggatatgat tacagcagga atggacacaa 960 caaccataac agtggaatgg gcaatggcag aactagttaa gaacccaaga gtgcaactaa 1020 aagctcaaga ggagcttgac agggtaatcg gaacggatcg aatcatgtca gaaaccgatt 1080 tctctaaact tccttaccta caatgtgtag ccaaagaggc tctaaggttg caccctccaa 1140 ctcctctaat gcttcctcat agggccagtg ccagtgtcaa aattggtggt tatgacattc 1200 ctaaggggtc catcgtgcac gtgaacgttt gggctgtcgc tcgtgaccca gccgtgtgga 1260 agaacccgtt ggagttcaga ccagagcgct tccttgagga agacgttgac atgaagggtc 1320 acgactatcg gttattgccc tttggtgcag gaaggcgtgt ttgccccggt gcacaacttg 1380 ctatcaactt ggtcacatct atgttgggtc atttgttgca tcattttaca tgggctccgg 1440 ccccgggggt taacccggag gatattgact tggaggagag ccctggaaca gtaacttaca 1500 tgaaaaatcc aatacaagct attccaactc caagattgcc tgcacacttg tatggacgtg 1560 tgccagtgga tatgtaaaac 1580 256 520 PRT NICOTIANATABACUM 256 Met Tyr Ser His Arg Ser Lys Val Ser Thr Lys Ile Met Ala Leu Ser 1 5 10 15 Phe Ile Phe Ile Ser Ile Thr Leu Ile Phe Leu Val His Lys Leu Tyr 20 25 30 His Arg Leu Arg Phe Lys Leu Pro Pro Gly Pro Arg Pro Leu Pro Val 35 40 45 Val Gly Asn Leu Tyr Asp Ile Glu Pro Val Arg Phe Arg Cys Phe Ala 50 55 60 Asp Trp Ala Lys Thr Tyr Gly Pro Ile Phe Ser Val Tyr Phe Gly Ser 65 70 75 80 Gln Leu Asn Val Val Val Thr Thr Ala Glu Leu Ala Lys Glu Val Leu 85 90 95 Lys Glu Asn Asp Gln Asn Leu Ala Asp Arg Phe Arg Thr Arg Pro Ala 100 105 110 Asn Asn Leu Ser Arg Asn Gly Met Asp Leu Ile Trp Ala Asp Tyr Gly 115 120 125 Pro His Tyr Val Lys Val Arg Lys Leu Cys Asn Leu Glu Leu Phe Thr 130 135 140 Pro Lys Arg Leu Glu Ala Leu Arg Pro Ile Arg Glu Asp Glu Val Thr 145 150 155 160 Ala Met Val Glu Asn Ile Phe Lys Asp Cys Thr Lys Pro Asp Asn Thr 165 170 175 Gly Lys Ser Leu Leu Ile Arg Glu Tyr Leu Gly Ser Val Ala Phe Asn 180 185 190 Asn Ile Thr Arg Leu Thr Phe Gly Lys Arg Phe Met Asn Ser Lys Gly 195 200 205 Glu Ile Asp Glu Gln Gly Gln Glu Phe Lys Gly Ile Val Ser Asn Gly 210 215 220 Ile Lys Ile Gly Gly Lys Leu Pro Leu Ala Glu Tyr Val Pro Trp Leu 225 230 235 240 Arg Trp Phe Phe Thr Met Glu Asn Glu Ala Leu Val Lys His Ser Ala 245 250 255 Arg Arg Asp Arg Leu Thr Arg Met Ile Met Asp Glu His Thr Leu Ala 260 265 270 Arg Lys Lys Thr Gly Asp Thr Lys Gln His Phe Val Asp Ala Leu Leu 275 280 285 Thr Leu Gln Lys Gln Tyr Asp Leu Ser Asp Asp Thr Val Ile Gly Leu 290 295 300 Leu Trp Asp Met Ile Thr Ala Gly Met Asp Thr Thr Thr Ile Thr Val 305 310 315 320 Glu Trp Ala Met Ala Glu Leu Val Lys Asn Pro Arg Val Gln Leu Lys 325 330 335 Ala Gln Glu Glu Leu Asp Arg Val Ile Gly Thr Asp Arg Ile Met Ser 340 345 350 Glu Thr Asp Phe Ser Lys Leu Pro Tyr Leu Gln Cys Val Ala Lys Glu 355 360 365 Ala Leu Arg Leu His Pro Pro Thr Pro Leu Met Leu Pro His Arg Ala 370 375 380 Ser Ala Ser Val Lys Ile Gly Gly Tyr Asp Ile Pro Lys Gly Ser Ile 385 390 395 400 Val His Val Asn Val Trp Ala Val Ala Arg Asp Pro Ala Val Trp Lys 405 410 415 Asn Pro Leu Glu Phe Arg Pro Glu Arg Phe Leu Glu Glu Asp Val Asp 420 425 430 Met Lys Gly His Asp Tyr Arg Leu Leu Pro Phe Gly Ala Gly Arg Arg 435 440 445 Val Cys Pro Gly Ala Gln Leu Ala Ile Asn Leu Val Thr Ser Met Leu 450 455 460 Gly His Leu Leu His His Phe Thr Trp Ala Pro Ala Pro Gly Val Asn 465 470 475 480 Pro Glu Asp Ile Asp Leu Glu Glu Ser Pro Gly Thr Val Thr Tyr Met 485 490 495 Lys Asn Pro Ile Gln Ala Ile Pro Thr Pro Arg Leu Pro Ala His Leu 500 505 510 Tyr Gly Arg Val Pro Val Asp Met 515 520 257 1544 DNA NICOTIANATABACUM 257 ataatgctct ttctactctt tgtagccctt cctttcattc ttatttttct tcttcctaaa 60 ttcaaaaatg gtggaaataa cagattgcca ccaggtccta taggtttacc attcattgga 120 aatttgcatc aatatgatag tataactcct catatctatt tttggaaact ttccaaaaaa 180 tatggcaaaa tcttctcatt aaaacttgct tctactaatg tggtagtagt ttcttcagca 240 aaattagcaa aagaagtatt gaaaaaacaa gatttaatat tttgtagtag accatctatt 300 cttggccaac aaaaactgtc ttattatggt cgtgatattg cttttgcacc ttataatgat 360 tattggagag aaatgagaaa aatttgtgtt cttcatcttt ttagtttaaa aaaagttcaa 420 ttatttagtc caattcgtga agatgaagtt tttagaatga ttaagaaaat atcaaaacaa 480 gcttctactt cacaaattat taatttgagt aatttaatga tttcattaac aagtacaatt 540 atttgtagag ttgcttttgg tgttaggttt gaagaagaag cacatgcaag gaagagattt 600 gattttcttt tggccgaggc acaagaaatg atggctagtt tctttgtatc tgattttttt 660 ccctttttaa gttagattga caaattaagt ggattgacat atagacttga gaggaatttc 720 aaggatttgg ataattttta tgaagaactc attgagcaac atcaaaatcc taataagcca 780 aaatatatgg aaggagatat tgttgatctt ttgctacaat tgaagaaaga gaaattaaca 840 ccacttgatc tcactatgga agatataaaa ggaattctca tgaatgtgtt agttgcagga 900 tcagacacta gtgcagctgc tactgtttgg gcaatgacag ccttgataaa gaatcctaaa 960 gccatggaaa aagttcaatt agaaatcaga aaatcagttg ggaagaaagg cattgtaaat 1020 gaagaagatg tccaaaacat cccttatttt aaagcagtga taaaggaaat atttagattg 1080 tatccaccag ctccactttt agttccaaga gaatcaatgg aaaaaaccat attagaaggt 1140 tatgaaattc ggccaagaac catagttcat gttaacgctt gggctatagc aagggatcct 1200 gaaatatggg aaaatccaga tgaatttata cctgagagat ttttgaatag cagtatcgat 1260 tacaagggtc aagattttga gttacttcca tttggtgcag gcagaagagg ttgcccaggt 1320 attgcacttg gggttgcatc catggaactt gctttgtcaa atcttcttta tgcatttgat 1380 tgggagttgc cttatggagt gaaaaaagaa gacatcgaca caaacgttag gcctggaatt 1440 gccatgcaca agaaaaacga actttgcctt gtcccaaaaa aattatttat aaattatatt 1500 gggacgtgga tctcatgcta gttctgtgcg gtcagctaag ctta 1544 258 504 PRT NICOTIANATABACUM 258 Met Leu Phe Leu Leu Phe Val Ala Leu Pro Phe Ile Leu Ile Phe Leu 1 5 10 15 Leu Pro Lys Phe Lys Asn Gly Gly Asn Asn Arg Leu Pro Pro Gly Pro 20 25 30 Ile Gly Leu Pro Phe Ile Gly Asn Leu His Gln Tyr Asp Ser Ile Thr 35 40 45 Pro His Ile Tyr Phe Trp Lys Leu Ser Lys Lys Tyr Gly Lys Ile Phe 50 55 60 Ser Leu Lys Leu Ala Ser Thr Asn Val Val Val Val Ser Ser Ala Lys 65 70 75 80 Leu Ala Lys Glu Val Leu Lys Lys Gln Asp Leu Ile Phe Cys Ser Arg 85 90 95 Pro Ser Ile Leu Gly Gln Gln Lys Leu Ser Tyr Tyr Gly Arg Asp Ile 100 105 110 Ala Phe Ala Pro Tyr Asn Asp Tyr Trp Arg Glu Met Arg Lys Ile Cys 115 120 125 Val Leu His Leu Phe Ser Leu Lys Lys Val Gln Leu Phe Ser Pro Ile 130 135 140 Arg Glu Asp Glu Val Phe Arg Met Ile Lys Lys Ile Ser Lys Gln Ala 145 150 155 160 Ser Thr Ser Gln Ile Ile Asn Leu Ser Asn Leu Met Ile Ser Leu Thr 165 170 175 Ser Thr Ile Ile Cys Arg Val Ala Phe Gly Val Arg Phe Glu Glu Glu 180 185 190 Ala His Ala Arg Lys Arg Phe Asp Phe Leu Leu Ala Glu Ala Gln Glu 195 200 205 Met Met Ala Ser Phe Phe Val Ser Asp Phe Phe Pro Phe Leu Ser Ile 210 215 220 Asp Lys Leu Ser Gly Leu Thr Tyr Arg Leu Glu Arg Asn Phe Lys Asp 225 230 235 240 Leu Asp Asn Phe Tyr Glu Glu Leu Ile Glu Gln His Gln Asn Pro Asn 245 250 255 Lys Pro Lys Tyr Met Glu Gly Asp Ile Val Asp Leu Leu Leu Gln Leu 260 265 270 Lys Lys Glu Lys Leu Thr Pro Leu Asp Leu Thr Met Glu Asp Ile Lys 275 280 285 Gly Ile Leu Met Asn Val Leu Val Ala Gly Ser Asp Thr Ser Ala Ala 290 295 300 Ala Thr Val Trp Ala Met Thr Ala Leu Ile Lys Asn Pro Lys Ala Met 305 310 315 320 Glu Lys Val Gln Leu Glu Ile Arg Lys Ser Val Gly Lys Lys Gly Ile 325 330 335 Val Asn Glu Glu Asp Val Gln Asn Ile Pro Tyr Phe Lys Ala Val Ile 340 345 350 Lys Glu Ile Phe Arg Leu Tyr Pro Pro Ala Pro Leu Leu Val Pro Arg 355 360 365 Glu Ser Met Glu Lys Thr Ile Leu Glu Gly Tyr Glu Ile Arg Pro Arg 370 375 380 Thr Ile Val His Val Asn Ala Trp Ala Ile Ala Arg Asp Pro Glu Ile 385 390 395 400 Trp Glu Asn Pro Asp Glu Phe Ile Pro Glu Arg Phe Leu Asn Ser Ser 405 410 415 Ile Asp Tyr Lys Gly Gln Asp Phe Glu Leu Leu Pro Phe Gly Ala Gly 420 425 430 Arg Arg Gly Cys Pro Gly Ile Ala Leu Gly Val Ala Ser Met Glu Leu 435 440 445 Ala Leu Ser Asn Leu Leu Tyr Ala Phe Asp Trp Glu Leu Pro Tyr Gly 450 455 460 Val Lys Lys Glu Asp Ile Asp Thr Asn Val Arg Pro Gly Ile Ala Met 465 470 475 480 His Lys Lys Asn Glu Leu Cys Leu Val Pro Lys Lys Leu Phe Ile Asn 485 490 495 Tyr Ile Gly Thr Trp Ile Ser Cys 500 259 1615 DNA NICOTIANATABACUM 259 cacattgagt cctctcccaa atcactgatt caccaccaaa agtaccaaca attcaatgga 60 aggtacaaac ttgactacat atgcagcagt atttcttgat actctgtttc ttttgttcct 120 ttccaaactt cttcgccaga ggaaactcaa tttacctcca ggcccaaaac catggccgat 180 catcggaaac ttaaacctta ttggcaatct tcctcatcgc tcaatccacg aactctccct 240 caagtacgga cccgttatgc aactccaatt cgggtctttc cccgttgtag ttggatcctc 300 cgtcgaaatg gctaagattt tcctcaaatc catggatatt aactttgtag gcaggcctaa 360 aacggctgcc ggaaaataca caacgtacaa ttattccgat attacatggt ctccttacgg 420 accatattgg cgccaggcac gtaggatgtg cctaacggaa ttattcagca cgaaacgtct 480 cgattcatac gagtatattc gggctgagga gttgcattct cttctccata atttgaacaa 540 aatatcaggg aaaccaattg tgttgaaaga ttatttgacg acgttgagtt taaatgttat 600 tagcaggatg gtactgggga aaaggtattt ggacgaatcc gagaactcgt tcgtgaatcc 660 tgaggaattt aagaagatgt tggacgaatt gtttttgcta aatggtgtac ttaatattgg 720 agattcaatt ccatggattg atttcatgga tttgcaaggt tatgttaaga ggatgaaagt 780 agtgagcaag aaattcgaca agtttttaga gcatgttatt gatgagcata acattaggag 840 aaatggagtg gagaattatg ttgctaagga tatggtggat gttttgttgc agcttgctga 900 tgatccgaag ttggaagtta agctggagag acatggagtc aaagcattca ctcaggatat 960 gctggctggt ggaaccgaga gttcagcagt gacagtggag tgggcaattt cagagctgct 1020 aaagaagccg gagattttca aaaaggctac agaagaattg gatcgagtaa ttgggcagaa 1080 tagatgggta caagaaaagg acattccaaa tcttccttac atagaggcaa tagtcaaaga 1140 gactatgcga ctgcaccccg tggcaccaat gttggtgcca cgtgagtgtc gagaagatat 1200 taaggtagca ggctacgacg ttcagaaagg aactagggtt ctcgtgagtg tatggactat 1260 tggaagagac cctacattgt gggacgagcc tgaggtgttc aagccggaga gattccatga 1320 aaagtccata gatgttaaag gacatgatta tgagcttttg ccatttggag cggggagaag 1380 aatgtgcccg ggttatagct tggggctcaa ggtgattcaa gctagcttag ctaatcttct 1440 acatggattt aactggtcat tgcctgataa tatgactcct gaggacctca acatggatga 1500 gatttttggg ctctctacac ctaaaaaatt tccacttgct actgtgattg agccaagact 1560 ttcaccaaaa ctttactctg tttgattcag cagttctatg gttccgtcaa gatag 1615 260 509 PRT NICOTIANATABACUM 260 Met Glu Gly Thr Asn Leu Thr Thr Tyr Ala Ala Val Phe Leu Asp Thr 1 5 10 15 Leu Phe Leu Leu Phe Leu Ser Lys Leu Leu Arg Gln Arg Lys Leu Asn 20 25 30 Leu Pro Pro Gly Pro Lys Pro Trp Pro Ile Ile Gly Asn Leu Asn Leu 35 40 45 Ile Gly Asn Leu Pro His Arg Ser Ile His Glu Leu Ser Leu Lys Tyr 50 55 60 Gly Pro Val Met Gln Leu Gln Phe Gly Ser Phe Pro Val Val Val Gly 65 70 75 80 Ser Ser Val Glu Met Ala Lys Ile Phe Leu Lys Ser Met Asp Ile Asn 85 90 95 Phe Val Gly Arg Pro Lys Thr Ala Ala Gly Lys Tyr Thr Thr Tyr Asn 100 105 110 Tyr Ser Asp Ile Thr Trp Ser Pro Tyr Gly Pro Tyr Trp Arg Gln Ala 115 120 125 Arg Arg Met Cys Leu Thr Glu Leu Phe Ser Thr Lys Arg Leu Asp Ser 130 135 140 Tyr Glu Tyr Ile Arg Ala Glu Glu Leu His Ser Leu Leu His Asn Leu 145 150 155 160 Asn Lys Ile Ser Gly Lys Pro Ile Val Leu Lys Asp Tyr Leu Thr Thr 165 170 175 Leu Ser Leu Asn Val Ile Ser Arg Met Val Leu Gly Lys Arg Tyr Leu 180 185 190 Asp Glu Ser Glu Asn Ser Phe Val Asn Pro Glu Glu Phe Lys Lys Met 195 200 205 Leu Asp Glu Leu Phe Leu Leu Asn Gly Val Leu Asn Ile Gly Asp Ser 210 215 220 Ile Pro Trp Ile Asp Phe Met Asp Leu Gln Gly Tyr Val Lys Arg Met 225 230 235 240 Lys Val Val Ser Lys Lys Phe Asp Lys Phe Leu Glu His Val Ile Asp 245 250 255 Glu His Asn Ile Arg Arg Asn Gly Val Glu Asn Tyr Val Ala Lys Asp 260 265 270 Met Val Asp Val Leu Leu Gln Leu Ala Asp Asp Pro Lys Leu Glu Val 275 280 285 Lys Leu Glu Arg His Gly Val Lys Ala Phe Thr Gln Asp Met Leu Ala 290 295 300 Gly Gly Thr Glu Ser Ser Ala Val Thr Val Glu Trp Ala Ile Ser Glu 305 310 315 320 Leu Leu Lys Lys Pro Glu Ile Phe Lys Lys Ala Thr Glu Glu Leu Asp 325 330 335 Arg Val Ile Gly Gln Asn Arg Trp Val Gln Glu Lys Asp Ile Pro Asn 340 345 350 Leu Pro Tyr Ile Glu Ala Ile Val Lys Glu Thr Met Arg Leu His Pro 355 360 365 Val Ala Pro Met Leu Val Pro Arg Glu Cys Arg Glu Asp Ile Lys Val 370 375 380 Ala Gly Tyr Asp Val Gln Lys Gly Thr Arg Val Leu Val Ser Val Trp 385 390 395 400 Thr Ile Gly Arg Asp Pro Thr Leu Trp Asp Glu Pro Glu Val Phe Lys 405 410 415 Pro Glu Arg Phe His Glu Lys Ser Ile Asp Val Lys Gly His Asp Tyr 420 425 430 Glu Leu Leu Pro Phe Gly Ala Gly Arg Arg Met Cys Pro Gly Tyr Ser 435 440 445 Leu Gly Leu Lys Val Ile Gln Ala Ser Leu Ala Asn Leu Leu His Gly 450 455 460 Phe Asn Trp Ser Leu Pro Asp Asn Met Thr Pro Glu Asp Leu Asn Met 465 470 475 480 Asp Glu Ile Phe Gly Leu Ser Thr Pro Lys Lys Phe Pro Leu Ala Thr 485 490 495 Val Ile Glu Pro Arg Leu Ser Pro Lys Leu Tyr Ser Val 500 505 261 1610 DNA NICOTIANATABACUM 261 cttcttcctt cctaactaaa aatggagatt cagttttcta acttagttgc attcttgctc 60 tttctctcca gcatctttct tgtattcaaa aaatggaaaa ccagaaaact aaatttgcct 120 cctggtccat ggaaattacc ttttattgga agtttacacc atttggctgt ggcaggtcca 180 cttcctcacc atggcctaaa aaatttagcc aaacgctatg gtcctcttat gcatttacaa 240 cttggacaaa ttcctacact cgtcatatca tcacctcaaa tggcaaaaga agtactaaaa 300 actcacgacc tcgcttttgc cactagacca aagcttgtcg tggccgacat cattcactac 360 gacagcacgg acatagcact ttcgccatac ggtgaatact ggagacaaat tcgtaaaatt 420 tgcatattgg aactcttgag tgccaagatg gtcaagtttt ttagctcgat tcgccaagat 480 gagctctcga agatggtttc atctatacga acgacgccca atcttccagt caatcttacc 540 gacaagattt tttggtttac gagttcggta atttgtagat cagctttagg gaagatatgt 600 ggtgaccaag acaaattgat catttttatg agggaaataa tatcattggc aggtggattt 660 agtattgctg attttttccc tacatggaaa atgattcatg atattgatgg ttcaaaatct 720 aaactggtga aggcacatcg taagattgat gaaattttgg aaaatgtggt aaatgagcac 780 aaacagaatc gagcagatgg taaaaagggt aatggtgaat ttggtggaga agatctgatt 840 gatgttttgt taagagttag agaaagtgga gaagttcaaa ttccaatcac agatgacaat 900 atcaaatcaa tattaatcga catgttctct gccggatcgg aaacatcatc gacaactata 960 atttgggcat tagctgaaat gatgaagaaa ccaagtgttt tagcaaaggc acaagctgaa 1020 gtgagccaag ctttgaaggg gaagaaaatt agttttcaag agattgatat tgataagcta 1080 aagtatttga agttagtgat caaagaaact ttaagaatgc accctccaat tcctctgtta 1140 gtccctagag aatgtatgga agatacaaag attgatggtt acaatatacc tttcaaaaca 1200 agagtcattg ttaatgcatg ggcaattgga cgagatcctc aaagttggga tgatcctgaa 1260 agctttacgc cagagagatt tgagaataat tctattgatt ttcttggaaa tcatcatcaa 1320 tttattccat ttggtgcagg aagaaggatt tgtcctggaa tgctatttgg tttagctaat 1380 gttggacaac ctttagctca gttactttat cacttcgatt ggaaactccc taatggacaa 1440 actcaccaaa atttcgacat gactgagtca cctggaattt ctgctacaag aaaggatgat 1500 cttattttga ttgccactcc tgctcattct tgattaagta ttgctgcttt tctattggag 1560 aattttcaaa attcatccac aatatatagt gtttgctaga gttggttagc 1610 262 503 PRT NICOTIANATABACUM 262 Met Glu Ile Gln Phe Ser Asn Leu Val Ala Phe Leu Leu Phe Leu Ser 1 5 10 15 Ser Ile Phe Leu Val Phe Lys Lys Trp Lys Thr Arg Lys Leu Asn Leu 20 25 30 Pro Pro Gly Pro Trp Lys Leu Pro Phe Ile Gly Ser Leu His His Leu 35 40 45 Ala Val Ala Gly Pro Leu Pro His His Gly Leu Lys Asn Leu Ala Lys 50 55 60 Arg Tyr Gly Pro Leu Met His Leu Gln Leu Gly Gln Ile Pro Thr Leu 65 70 75 80 Val Ile Ser Ser Pro Gln Met Ala Lys Glu Val Leu Lys Thr His Asp 85 90 95 Leu Ala Phe Ala Thr Arg Pro Lys Leu Val Val Ala Asp Ile Ile His 100 105 110 Tyr Asp Ser Thr Asp Ile Ala Leu Ser Pro Tyr Gly Glu Tyr Trp Arg 115 120 125 Gln Ile Arg Lys Ile Cys Ile Leu Glu Leu Leu Ser Ala Lys Met Val 130 135 140 Lys Phe Phe Ser Ser Ile Arg Gln Asp Glu Leu Ser Lys Met Val Ser 145 150 155 160 Ser Ile Arg Thr Thr Pro Asn Leu Pro Val Asn Leu Thr Asp Lys Ile 165 170 175 Phe Trp Phe Thr Ser Ser Val Ile Cys Arg Ser Ala Leu Gly Lys Ile 180 185 190 Cys Gly Asp Gln Asp Lys Leu Ile Ile Phe Met Arg Glu Ile Ile Ser 195 200 205 Leu Ala Gly Gly Phe Ser Ile Ala Asp Phe Phe Pro Thr Trp Lys Met 210 215 220 Ile His Asp Ile Asp Gly Ser Lys Ser Lys Leu Val Lys Ala His Arg 225 230 235 240 Lys Ile Asp Glu Ile Leu Glu Asn Val Val Asn Glu His Lys Gln Asn 245 250 255 Arg Ala Asp Gly Lys Lys Gly Asn Gly Glu Phe Gly Gly Glu Asp Leu 260 265 270 Ile Asp Val Leu Leu Arg Val Arg Glu Ser Gly Glu Val Gln Ile Pro 275 280 285 Ile Thr Asp Asp Asn Ile Lys Ser Ile Leu Ile Asp Met Phe Ser Ala 290 295 300 Gly Ser Glu Thr Ser Ser Thr Thr Ile Ile Trp Ala Leu Ala Glu Met 305 310 315 320 Met Lys Lys Pro Ser Val Leu Ala Lys Ala Gln Ala Glu Val Ser Gln 325 330 335 Ala Leu Lys Gly Lys Lys Ile Ser Phe Gln Glu Ile Asp Ile Asp Lys 340 345 350 Leu Lys Tyr Leu Lys Leu Val Ile Lys Glu Thr Leu Arg Met His Pro 355 360 365 Pro Ile Pro Leu Leu Val Pro Arg Glu Cys Met Glu Asp Thr Lys Ile 370 375 380 Asp Gly Tyr Asn Ile Pro Phe Lys Thr Arg Val Ile Val Asn Ala Trp 385 390 395 400 Ala Ile Gly Arg Asp Pro Gln Ser Trp Asp Asp Pro Glu Ser Phe Thr 405 410 415 Pro Glu Arg Phe Glu Asn Asn Ser Ile Asp Phe Leu Gly Asn His His 420 425 430 Gln Phe Ile Pro Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly Met Leu 435 440 445 Phe Gly Leu Ala Asn Val Gly Gln Pro Leu Ala Gln Leu Leu Tyr His 450 455 460 Phe Asp Trp Lys Leu Pro Asn Gly Gln Thr His Gln Asn Phe Asp Met 465 470 475 480 Thr Glu Ser Pro Gly Ile Ser Ala Thr Arg Lys Asp Asp Leu Ile Leu 485 490 495 Ile Ala Thr Pro Ala His Ser 500 263 1611 DNA NICOTIANATABACUM 263 ctttcatcat atggcatgaa atgggaaatg ctcacaacag caaaattgca gcaatctgtt 60 tgataatttt cttggtatat aaagcatggg aattgttgaa gtggatatgg attaagccaa 120 agaaactgga gagttgcctc agaaaacagg gactcaaagg aaattcctac gggctattct 180 atggagatat gaaagaattg tccaaaagtc tcaaggaaat caattcaaag cccatcatca 240 atctatcaaa tgaagtagcc ccaagaatca ttccttatta tcttgaaatc atccaaaaat 300 atggtaaaag atgttttgtt tggcaaggac caacccccgc aatattaata acagagccag 360 aattaataaa ggagatattt ggtaagaact atgtttttca gaagcctaat aatcccaacc 420 cactgaccaa gttattggct cgaggtgttg taagctacga ggaagaaaaa tgggcaaaac 480 acagaaagat cttaaatcct gcctttcata tggagaagtt gaagcatatg ctaccagcat 540 tttacttgag ctgtagtgag atgctgaaca aatgggagga gattatccca gtaaaagaat 600 caaatgagtt ggacatttgg cctcatcttc aaagaatgac aagtgatgtg atttctcgtg 660 ctgcctttgg tagtagctac gaagaaggaa gaagaatatt tgaacttcaa gaagaacaag 720 ctgagtatct aacgaagaca ttcaattcag tttatatccc aggttccaga ttttttccca 780 ataaaatgaa caaaagaatg aaagaatgtg aaaaggaagt acgagaaaca attacgtgtc 840 taattgacaa cagattaaag gcaaaagaag aaggcaatgg caaggccctc aatgatgacc 900 tattgggtat attattagag tcaaattcta tagaaattga agaacatggt aacaagaagt 960 ttggaatgag tatacctgaa gtaattgaag agtgcaaatt attctatttt gctggccaag 1020 agactacatc agtattgctt gtgtggacac tgattttgtt agggagaaat ccagaatggc 1080 aggaacgtgc tagagaggaa gtttttcaag cctttggaag tgataaacca acttttgacg 1140 aattatatcg cttgaaaatt gtgacgatga ttttgtacga gtctttaagg ttatatccac 1200 caatagcaac tcgtactcga aggactaatg aagaaacaaa attaggggaa ctagatttac 1260 caaagggtgc actgctcttt ataccaacaa tcttattaca tcttgacagg gaaatttggg 1320 gtgaagatgc agatgagttc aatccggaga gatttagcga aggggtggca aaggcaacaa 1380 aggggaaaat gacatatttt ccatttggtg caggaccgcg aaaatgcatt gggcaaaact 1440 tcgcgatttt ggaagcaaaa atggctatag ctatgattct acaacgcttc tccttcgagc 1500 tctctccatc ttatacacac tctccataca ctgtggtcac tttgaaaccc aaatatggtg 1560 ctcccctaat aatgcacagg ctgtagtcct gtgagaatat gctatccgag g 1611 264 521 PRT NICOTIANATABACUM 264 Met Gly Asn Ala His Asn Ser Lys Ile Ala Ala Ile Cys Leu Ile Ile 1 5 10 15 Phe Leu Val Tyr Lys Ala Trp Glu Leu Leu Lys Trp Ile Trp Ile Lys 20 25 30 Pro Lys Lys Leu Glu Ser Cys Leu Arg Lys Gln Gly Leu Lys Gly Asn 35 40 45 Ser Tyr Gly Leu Phe Tyr Gly Asp Met Lys Glu Leu Ser Lys Ser Leu 50 55 60 Lys Glu Ile Asn Ser Lys Pro Ile Ile Asn Leu Ser Asn Glu Val Ala 65 70 75 80 Pro Arg Ile Ile Pro Tyr Tyr Leu Glu Ile Ile Gln Lys Tyr Gly Lys 85 90 95 Arg Cys Phe Val Trp Gln Gly Pro Thr Pro Ala Ile Leu Ile Thr Glu 100 105 110 Pro Glu Leu Ile Lys Glu Ile Phe Gly Lys Asn Tyr Val Phe Gln Lys 115 120 125 Pro Asn Asn Pro Asn Pro Leu Thr Lys Leu Leu Ala Arg Gly Val Val 130 135 140 Ser Tyr Glu Glu Glu Lys Trp Ala Lys His Arg Lys Ile Leu Asn Pro 145 150 155 160 Ala Phe His Met Glu Lys Leu Lys His Met Leu Pro Ala Phe Tyr Leu 165 170 175 Ser Cys Ser Glu Met Leu Asn Lys Trp Glu Glu Ile Ile Pro Val Lys 180 185 190 Glu Ser Asn Glu Leu Asp Ile Trp Pro His Leu Gln Arg Met Thr Ser 195 200 205 Asp Val Ile Ser Arg Ala Ala Phe Gly Ser Ser Tyr Glu Glu Gly Arg 210 215 220 Arg Ile Phe Glu Leu Gln Glu Glu Gln Ala Glu Tyr Leu Thr Lys Thr 225 230 235 240 Phe Asn Ser Val Tyr Ile Pro Gly Ser Arg Phe Phe Pro Asn Lys Met 245 250 255 Asn Lys Arg Met Lys Glu Cys Glu Lys Glu Val Arg Glu Thr Ile Thr 260 265 270 Cys Leu Ile Asp Asn Arg Leu Lys Ala Lys Glu Glu Gly Asn Gly Lys 275 280 285 Ala Leu Asn Asp Asp Leu Leu Gly Ile Leu Leu Glu Ser Asn Ser Ile 290 295 300 Glu Ile Glu Glu His Gly Asn Lys Lys Phe Gly Met Ser Ile Pro Glu 305 310 315 320 Val Ile Glu Glu Cys Lys Leu Phe Tyr Phe Ala Gly Gln Glu Thr Thr 325 330 335 Ser Val Leu Leu Val Trp Thr Leu Ile Leu Leu Gly Arg Asn Pro Glu 340 345 350 Trp Gln Glu Arg Ala Arg Glu Glu Val Phe Gln Ala Phe Gly Ser Asp 355 360 365 Lys Pro Thr Phe Asp Glu Leu Tyr Arg Leu Lys Ile Val Thr Met Ile 370 375 380 Leu Tyr Glu Ser Leu Arg Leu Tyr Pro Pro Ile Ala Thr Arg Thr Arg 385 390 395 400 Arg Thr Asn Glu Glu Thr Lys Leu Gly Glu Leu Asp Leu Pro Lys Gly 405 410 415 Ala Leu Leu Phe Ile Pro Thr Ile Leu Leu His Leu Asp Arg Glu Ile 420 425 430 Trp Gly Glu Asp Ala Asp Glu Phe Asn Pro Glu Arg Phe Ser Glu Gly 435 440 445 Val Ala Lys Ala Thr Lys Gly Lys Met Thr Tyr Phe Pro Phe Gly Ala 450 455 460 Gly Pro Arg Lys Cys Ile Gly Gln Asn Phe Ala Ile Leu Glu Ala Lys 465 470 475 480 Met Ala Ile Ala Met Ile Leu Gln Arg Phe Ser Phe Glu Leu Ser Pro 485 490 495 Ser Tyr Thr His Ser Pro Tyr Thr Val Val Thr Leu Lys Pro Lys Tyr 500 505 510 Gly Ala Pro Leu Ile Met His Arg Leu 515 520 265 1570 DNA NICOTIANATABACUM 265 ctcattatcc atcacctaaa atggagaatt cttgggtttt tctagccttg gcagggctat 60 ctgcattagc ttttctctgt aaaataatca cctgtcgaag accggttaac cggaaaatac 120 caccaggtcc aaaaccatgg cccatcattg gcaatttgaa cctacttggt cctataccac 180 atcaatcttt tgacttgctt tccaaaaaat atggagagtt gatgctgctg aaatttggct 240 ccaggccagt tcttgttgct tcatctgctg aaatggcaaa acagttttta aaagtacatg 300 atgctaattt cgcctcccgt cctatgctag ctggtggaaa gtatacaagc tataactatt 360 gtgacatgac atgggcaccc tatggtccct attggcgcca agcacgacga atttacctta 420 accagatatt tactccgaaa aggctagact cgttcgagta cattcgtgtt gaagaaaggc 480 aggccttgat ttcccagctg aattcccttg ctggaaagcc attttttctc aaagaccatt 540 tgtcgcgatt tagcctctgc agcatgacaa ggatggtttt gagcaacaag tattttggtg 600 aatcaacagt tagagtagaa gatttgcagt acctggtaga tcaatggttc ttacttaatg 660 gtgctttcaa cattggagat tggattccat ggctcagctt cttggaccta caaggctatg 720 tgaaacaaat gaaggctttg aaaagaactt ttgataagtt ccacaacatt gtgctagatg 780 atcacagggc taagaagaat gcagagaaga actttgtccc aaaagacatg gttgatgtct 840 tgttgaagat ggctgaagat cctaatctgg aagtcaaact cactaatgac tgtgtcaaag 900 ggttaatgca ggatttacta actggaggaa cagatagctt aacagcagca gtgcaatggg 960 catttcaaga acttcttaga cagccaaggg ttattgagaa ggcaaccgaa gagcttgacc 1020 ggattgtcgg gaaagagaga tgggtagaag agaaagattg ctcgcagcta tcttacgttg 1080 aagcaatcct caaggaaaca ctaaggttac atcctctagg aactatgcta gcaccgcatt 1140 gtgctataga agattgtaac gtggctggtt atgacataca gaaaggaacg acctttctgg 1200 tgaatgtttg gaccattgga agggacccaa aatactggga tagagcacaa gagtttctcc 1260 ccgagagatt tttagagaac gacattgata tggacggaca taactttgct ttcttgccat 1320 ttggctcggg gcgaaggagg tgccctggct atagccttgg acttaaggtt atccgagtaa 1380 cattagccaa catgttgcat ggattcaact ggaaattacc tgaaggtatg aagccagaag 1440 atataagtgt ggaagaacat tatgggctca ctacacatcc taagtttcct gttcctgtga 1500 tcttggaatc tagactttct tcagatctct attcccccat cacttaatcc taagtgcttc 1560 ctattatagc 1570 266 508 PRT NICOTIANATABACUM 266 Met Glu Asn Ser Trp Val Phe Leu Ala Leu Ala Gly Leu Ser Ala Leu 1 5 10 15 Ala Phe Leu Cys Lys Ile Ile Thr Cys Arg Arg Pro Val Asn Arg Lys 20 25 30 Ile Pro Pro Gly Pro Lys Pro Trp Pro Ile Ile Gly Asn Leu Asn Leu 35 40 45 Leu Gly Pro Ile Pro His Gln Ser Phe Asp Leu Leu Ser Lys Lys Tyr 50 55 60 Gly Glu Leu Met Leu Leu Lys Phe Gly Ser Arg Pro Val Leu Val Ala 65 70 75 80 Ser Ser Ala Glu Met Ala Lys Gln Phe Leu Lys Val His Asp Ala Asn 85 90 95 Phe Ala Ser Arg Pro Met Leu Ala Gly Gly Lys Tyr Thr Ser Tyr Asn 100 105 110 Tyr Cys Asp Met Thr Trp Ala Pro Tyr Gly Pro Tyr Trp Arg Gln Ala 115 120 125 Arg Arg Ile Tyr Leu Asn Gln Ile Phe Thr Pro Lys Arg Leu Asp Ser 130 135 140 Phe Glu Tyr Ile Arg Val Glu Glu Arg Gln Ala Leu Ile Ser Gln Leu 145 150 155 160 Asn Ser Leu Ala Gly Lys Pro Phe Phe Leu Lys Asp His Leu Ser Arg 165 170 175 Phe Ser Leu Cys Ser Met Thr Arg Met Val Leu Ser Asn Lys Tyr Phe 180 185 190 Gly Glu Ser Thr Val Arg Val Glu Asp Leu Gln Tyr Leu Val Asp Gln 195 200 205 Trp Phe Leu Leu Asn Gly Ala Phe Asn Ile Gly Asp Trp Ile Pro Trp 210 215 220 Leu Ser Phe Leu Asp Leu Gln Gly Tyr Val Lys Gln Met Lys Ala Leu 225 230 235 240 Lys Arg Thr Phe Asp Lys Phe His Asn Ile Val Leu Asp Asp His Arg 245 250 255 Ala Lys Lys Asn Ala Glu Lys Asn Phe Val Pro Lys Asp Met Val Asp 260 265 270 Val Leu Leu Lys Met Ala Glu Asp Pro Asn Leu Glu Val Lys Leu Thr 275 280 285 Asn Asp Cys Val Lys Gly Leu Met Gln Asp Leu Leu Thr Gly Gly Thr 290 295 300 Asp Ser Leu Thr Ala Ala Val Gln Trp Ala Phe Gln Glu Leu Leu Arg 305 310 315 320 Gln Pro Arg Val Ile Glu Lys Ala Thr Glu Glu Leu Asp Arg Ile Val 325 330 335 Gly Lys Glu Arg Trp Val Glu Glu Lys Asp Cys Ser Gln Leu Ser Tyr 340 345 350 Val Glu Ala Ile Leu Lys Glu Thr Leu Arg Leu His Pro Leu Gly Thr 355 360 365 Met Leu Ala Pro His Cys Ala Ile Glu Asp Cys Asn Val Ala Gly Tyr 370 375 380 Asp Ile Gln Lys Gly Thr Thr Phe Leu Val Asn Val Trp Thr Ile Gly 385 390 395 400 Arg Asp Pro Lys Tyr Trp Asp Arg Ala Gln Glu Phe Leu Pro Glu Arg 405 410 415 Phe Leu Glu Asn Asp Ile Asp Met Asp Gly His Asn Phe Ala Phe Leu 420 425 430 Pro Phe Gly Ser Gly Arg Arg Arg Cys Pro Gly Tyr Ser Leu Gly Leu 435 440 445 Lys Val Ile Arg Val Thr Leu Ala Asn Met Leu His Gly Phe Asn Trp 450 455 460 Lys Leu Pro Glu Gly Met Lys Pro Glu Asp Ile Ser Val Glu Glu His 465 470 475 480 Tyr Gly Leu Thr Thr His Pro Lys Phe Pro Val Pro Val Ile Leu Glu 485 490 495 Ser Arg Leu Ser Ser Asp Leu Tyr Ser Pro Ile Thr 500 505 267 1617 DNA NICOTIANATABACUM 267 cacattgagt cctctcccaa atcactgatt caccaccaaa agtaccaaca attcaatgga 60 aggtacaaac ttgactacat atgcagcagt atttcttgat actctgtttc ttttgttcct 120 ttccaaactt cttcgccaga ggaaactcaa tttacctcca ggcccaaaac catggccgat 180 catcggaaac ttaaacctta ttggcaatct tcctcatcgc tcaatccacg aactctccct 240 caagtacgga cccgttatgc aactccaatt cgggtctttc cccgttgtag ttggatcctc 300 cgtcgaaatg gctaagattt tcctcaaatc catggatatt aactttgtag gcaggcctaa 360 aacggctgcc ggaaaataca caacgtacaa ttattccgat attacatggt ctccttacgg 420 accatattgg cgccaggcac gtaggatgtg cctaacggaa ttattcagca cgaaacgtct 480 cgattcatac gagtatattc gggctgagga gttgcattct cttctccata atttgaacaa 540 aatatcaggg aaaccaattg tgttgaaaga ttatttgacg acgttgagtt taaatgttat 600 tagcaggatg gtactgggga aaaggtattt ggacgaatcc gagaactcgt tcgtgaatcc 660 tgaggaattt aagaagatgt tggacgaatt gtttttgcta aatggtgtac ttaatattgg 720 agattcaatt ccatggattg atttcatgga tttgcaaggt tatgttaaga ggatgaaagt 780 agtgagcaag aaattcgaca agtttttaga gcatgttatt gatgagcata acattaggag 840 aaatggagtg gagaattatg ttgctaagga tatggtggat gttttgttgc agcttgctga 900 tgatccgaag ttggaagtta agctggagag acatggagtc aaagcattca ctcaggatat 960 gctggctggt ggaaccgaga gttcagcagt gacagtggag tgggcaattt cagagctgct 1020 aaagaagccg gagattttca aaaaggctac agaagaattg gatcgagtaa ttgggcagaa 1080 tagatgggta caagaaaagg acattccaaa tcatccttac atagaggcaa tagtcaaaga 1140 gactatgcga ctgcaccccg tggcaccaat gttggtgcca cgtgagtgtc gagaagatat 1200 taaggtagca ggctacgacg ttcagaaagg aactagggtt ctcgtgagtg tatggactat 1260 tggaagagac cctacattgt gggacgagcc tgaggtgttc aagccggaga gattccatga 1320 aaagtccata gatgttaaag gacatgatta tgagcttttg ccatttggag cggggagaag 1380 aatgtgcccg ggttatagct tggggctcaa ggtgattcaa gctagcttag ctaatcttct 1440 acatggattt aactggtcat tgcctgataa tatgactcct gaggacctca acatggatga 1500 gatttttggg ctctctacac ctaaaaaatt tccacttgct actgtgattg agccaagact 1560 ttcaccaaaa ctttactctg tttgattcag cagttctatg gatccgtcaa gatagac 1617 268 509 PRT NICOTIANATABACUM 268 Met Glu Gly Thr Asn Leu Thr Thr Tyr Ala Ala Val Phe Leu Asp Thr 1 5 10 15 Leu Phe Leu Leu Phe Leu Ser Lys Leu Leu Arg Gln Arg Lys Leu Asn 20 25 30 Leu Pro Pro Gly Pro Lys Pro Trp Pro Ile Ile Gly Asn Leu Asn Leu 35 40 45 Ile Gly Asn Leu Pro His Arg Ser Ile His Glu Leu Ser Leu Lys Tyr 50 55 60 Gly Pro Val Met Gln Leu Gln Phe Gly Ser Phe Pro Val Val Val Gly 65 70 75 80 Ser Ser Val Glu Met Ala Lys Ile Phe Leu Lys Ser Met Asp Ile Asn 85 90 95 Phe Val Gly Arg Pro Lys Thr Ala Ala Gly Lys Tyr Thr Thr Tyr Asn 100 105 110 Tyr Ser Asp Ile Thr Trp Ser Pro Tyr Gly Pro Tyr Trp Arg Gln Ala 115 120 125 Arg Arg Met Cys Leu Thr Glu Leu Phe Ser Thr Lys Arg Leu Asp Ser 130 135 140 Tyr Glu Tyr Ile Arg Ala Glu Glu Leu His Ser Leu Leu His Asn Leu 145 150 155 160 Asn Lys Ile Ser Gly Lys Pro Ile Val Leu Lys Asp Tyr Leu Thr Thr 165 170 175 Leu Ser Leu Asn Val Ile Ser Arg Met Val Leu Gly Lys Arg Tyr Leu 180 185 190 Asp Glu Ser Glu Asn Ser Phe Val Asn Pro Glu Glu Phe Lys Lys Met 195 200 205 Leu Asp Glu Leu Phe Leu Leu Asn Gly Val Leu Asn Ile Gly Asp Ser 210 215 220 Ile Pro Trp Ile Asp Phe Met Asp Leu Gln Gly Tyr Val Lys Arg Met 225 230 235 240 Lys Val Val Ser Lys Lys Phe Asp Lys Phe Leu Glu His Val Ile Asp 245 250 255 Glu His Asn Ile Arg Arg Asn Gly Val Glu Asn Tyr Val Ala Lys Asp 260 265 270 Met Val Asp Val Leu Leu Gln Leu Ala Asp Asp Pro Lys Leu Glu Val 275 280 285 Lys Leu Glu Arg His Gly Val Lys Ala Phe Thr Gln Asp Met Leu Ala 290 295 300 Gly Gly Thr Glu Ser Ser Ala Val Thr Val Glu Trp Ala Ile Ser Glu 305 310 315 320 Leu Leu Lys Lys Pro Glu Ile Phe Lys Lys Ala Thr Glu Glu Leu Asp 325 330 335 Arg Val Ile Gly Gln Asn Arg Trp Val Gln Glu Lys Asp Ile Pro Asn 340 345 350 His Pro Tyr Ile Glu Ala Ile Val Lys Glu Thr Met Arg Leu His Pro 355 360 365 Val Ala Pro Met Leu Val Pro Arg Glu Cys Arg Glu Asp Ile Lys Val 370 375 380 Ala Gly Tyr Asp Val Gln Lys Gly Thr Arg Val Leu Val Ser Val Trp 385 390 395 400 Thr Ile Gly Arg Asp Pro Thr Leu Trp Asp Glu Pro Glu Val Phe Lys 405 410 415 Pro Glu Arg Phe His Glu Lys Ser Ile Asp Val Lys Gly His Asp Tyr 420 425 430 Glu Leu Leu Pro Phe Gly Ala Gly Arg Arg Met Cys Pro Gly Tyr Ser 435 440 445 Leu Gly Leu Lys Val Ile Gln Ala Ser Leu Ala Asn Leu Leu His Gly 450 455 460 Phe Asn Trp Ser Leu Pro Asp Asn Met Thr Pro Glu Asp Leu Asn Met 465 470 475 480 Asp Glu Ile Phe Gly Leu Ser Thr Pro Lys Lys Phe Pro Leu Ala Thr 485 490 495 Val Ile Glu Pro Arg Leu Ser Pro Lys Leu Tyr Ser Val 500 505 269 1657 DNA NICOTIANATABACUM 269 tgcaatatag ttttcctagt cagttctagc ctccttttcc ttagaaataa tggattatca 60 tatttctttc cattttcaag ctcttttagg gcttttagcc tttgtgttct tgtctattat 120 cttatggaga agaacactca cttcaagaaa attagcccct gaaatcccag gggcatggcc 180 tattataggc catcttcgtc agctgagtgg tactgataag aatatcccat ttccccgaat 240 attgggcgct ttggcagata aatatggacc tgtcttcaca ctgagaatag ggatgtaccc 300 ctatttgatt gtcaacaatt gggaagcagc taaggattgt ctcacaacgc atgataagga 360 cttcgctgcc cgaccaactt ctatggctgg tgaaagcatc gggtacaagt atgcgaggtt 420 tacttatgct aattttggtc cttattataa ccaagtgcgc aaactagccc tacaacatgt 480 actctcgagt actaaactcg agaaaatgaa acacatacgt gtttctgaat tggaaactag 540 catcaaagaa ttatattctt tgacgctggg caaaaacaac atgcaaaaag tgaatataag 600 taaatggttt gaacaattga ctttaaacat aatcgtgaag acaatttgtg gcaagagata 660 tagcaacata gaggaggatg aagaggcaca acgtttcaga aaggcattta agggcatcat 720 gtttgttgta gggcaaattg ttttatatga cgcaattcca ttcccattgt tcaaatactt 780 tgatttccaa ggtcatatac aattgatgaa caaaatttat aaagacttag attctattct 840 tcaaggatgg ttggatgatc atatgatgaa caaggatgta aacaataagg atcaagatgc 900 catagatgcc atgcttaagg taacacaact taatgaattc aaagcctatg gtttttctca 960 ggccactgtg atcaagtcga cagtcttgag tttgatctta gatggaaatg acacaaccgc 1020 tgttcatttg atatgggtaa tgtccttatt actgaacaat ccacatgtta tgaaacaagg 1080 ccaagaagag atagacatga aagtgggtaa agagaggtgg attgaagata ctgacataaa 1140 aaatttagtg taccttcagg ctatcgttaa agagacattg cgcttgtatc cacctgttcc 1200 ttttctttta ccacacgaag cagtgcaaga ttgtaaagtg actggttacc acattcctaa 1260 aggtactcgt ctatatatca atgcgtggaa agtacatcgc gattctgaaa tttggtcaga 1320 gcccgaaaag tttatgccca atagattctt gactagcaaa gcaaatatag atgctcgcgg 1380 tcaaaatttt gaatttatac cgtttggttc tgggagacgg tcatgtccag ggttaggttt 1440 tgcgacttta gtgacacatc tgacttttgg tcgcttgctt caaggttttg attttagtaa 1500 gccatcaaac acgccaattg acatgacaga aggcgtaggc gttactttgc ctaaggttaa 1560 tcaagttgaa gttctaatta cccctcgttt accttctaag ctttatttat tttgaaagtg 1620 caaatcatca atcatgggtt gagtaattag tgatact 1657 270 521 PRT NICOTIANATABACUM 270 Met Asp Tyr His Ile Ser Phe His Phe Gln Ala Leu Leu Gly Leu Leu 1 5 10 15 Ala Phe Val Phe Leu Ser Ile Ile Leu Trp Arg Arg Thr Leu Thr Ser 20 25 30 Arg Lys Leu Ala Pro Glu Ile Pro Gly Ala Trp Pro Ile Ile Gly His 35 40 45 Leu Arg Gln Leu Ser Gly Thr Asp Lys Asn Ile Pro Phe Pro Arg Ile 50 55 60 Leu Gly Ala Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Leu Arg Ile 65 70 75 80 Gly Met Tyr Pro Tyr Leu Ile Val Asn Asn Trp Glu Ala Ala Lys Asp 85 90 95 Cys Leu Thr Thr His Asp Lys Asp Phe Ala Ala Arg Pro Thr Ser Met 100 105 110 Ala Gly Glu Ser Ile Gly Tyr Lys Tyr Ala Arg Phe Thr Tyr Ala Asn 115 120 125 Phe Gly Pro Tyr Tyr Asn Gln Val Arg Lys Leu Ala Leu Gln His Val 130 135 140 Leu Ser Ser Thr Lys Leu Glu Lys Met Lys His Ile Arg Val Ser Glu 145 150 155 160 Leu Glu Thr Ser Ile Lys Glu Leu Tyr Ser Leu Thr Leu Gly Lys Asn 165 170 175 Asn Met Gln Lys Val Asn Ile Ser Lys Trp Phe Glu Gln Leu Thr Leu 180 185 190 Asn Ile Ile Val Lys Thr Ile Cys Gly Lys Arg Tyr Ser Asn Ile Glu 195 200 205 Glu Asp Glu Glu Ala Gln Arg Phe Arg Lys Ala Phe Lys Gly Ile Met 210 215 220 Phe Val Val Gly Gln Ile Val Leu Tyr Asp Ala Ile Pro Phe Pro Leu 225 230 235 240 Phe Lys Tyr Phe Asp Phe Gln Gly His Ile Gln Leu Met Asn Lys Ile 245 250 255 Tyr Lys Asp Leu Asp Ser Ile Leu Gln Gly Trp Leu Asp Asp His Met 260 265 270 Met Asn Lys Asp Val Asn Asn Lys Asp Gln Asp Ala Ile Asp Ala Met 275 280 285 Leu Lys Val Thr Gln Leu Asn Glu Phe Lys Ala Tyr Gly Phe Ser Gln 290 295 300 Ala Thr Val Ile Lys Ser Thr Val Leu Ser Leu Ile Leu Asp Gly Asn 305 310 315 320 Asp Thr Thr Ala Val His Leu Ile Trp Val Met Ser Leu Leu Leu Asn 325 330 335 Asn Pro His Val Met Lys Gln Gly Gln Glu Glu Ile Asp Met Lys Val 340 345 350 Gly Lys Glu Arg Trp Ile Glu Asp Thr Asp Ile Lys Asn Leu Val Tyr 355 360 365 Leu Gln Ala Ile Val Lys Glu Thr Leu Arg Leu Tyr Pro Pro Val Pro 370 375 380 Phe Leu Leu Pro His Glu Ala Val Gln Asp Cys Lys Val Thr Gly Tyr 385 390 395 400 His Ile Pro Lys Gly Thr Arg Leu Tyr Ile Asn Ala Trp Lys Val His 405 410 415 Arg Asp Ser Glu Ile Trp Ser Glu Pro Glu Lys Phe Met Pro Asn Arg 420 425 430 Phe Leu Thr Ser Lys Ala Asn Ile Asp Ala Arg Gly Gln Asn Phe Glu 435 440 445 Phe Ile Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Leu Gly Phe 450 455 460 Ala Thr Leu Val Thr His Leu Thr Phe Gly Arg Leu Leu Gln Gly Phe 465 470 475 480 Asp Phe Ser Lys Pro Ser Asn Thr Pro Ile Asp Met Thr Glu Gly Val 485 490 495 Gly Val Thr Leu Pro Lys Val Asn Gln Val Glu Val Leu Ile Thr Pro 500 505 510 Arg Leu Pro Ser Lys Leu Tyr Leu Phe 515 520 271 1620 DNA NICOTIANATABACUM 271 agagagtgaa aatggacgca ctacttcaaa tgacagtaac agcatcttgt gctgccatag 60 taattactct gctggtgtgt atatggagag tgctgaactg gatttggttc agaccaaaga 120 aattggagtt gttgttgaga aaacaaggtt tggaaggaaa ttcttacaag gttttgtatg 180 gggacatgaa agagttttct gggatgatta aggaagcata ctcaaagcct atgagtctat 240 ctgatgatgt agcaccaaga ctgatgcctt tctttcttga aaccatcaaa aaatatggaa 300 aaagatcctt tatatggttt ggtccaagac cactagtatt gattatggat cctgagctta 360 taaaggaagt actctcaaaa atccatctgt atcaaaagcc tggtggaaat ccattagcaa 420 cactattggt acaaggaata gcaacctatg aggaagacaa atgggccaaa catagaaaaa 480 tcatcaatcc cgctttccat ctagagaagc taaagcttat gcttccagca tttcgcttaa 540 gctgtagtga gatgctgagc aaatgggaag acattgtttc agctgatagc tcacatgaga 600 tagatgtatg gtctcacctt gagcaattga cttgcgatgt gatctctcgg acagcttttg 660 gcagtagtta tgaagaaggt agaaagattt ttgaacttca aaaggaacaa gctcagtatc 720 ttgtggaagt tttccgctcc gtttatatcc caggaaggag atttttgcca acaaagagga 780 atagaagaat gaaggaaata aaaaaggatg tccgggcatc aattaaaggt attattgata 840 aaagattgaa ggcaatgaaa gcaggggaca ccaataatga ggatctattg ggtatattac 900 tggaatcgaa tattaaagaa attgaacagc acggaaacaa ggattttgga atgagcattg 960 aagaagtcat tgaagaatgc aagttattct attttgctgg ccaagaaact acatcagtgt 1020 tactcctatg gtctctagtg ttgttgagca ggtatcaaga ttggcaggca cgggccagag 1080 aagaaatctt gcaagtcttt ggcagtcgaa aaccagattt tgacggatta aatcatctaa 1140 aaattgtgac aatgatcttg tacgagtctt taaggctgta tccctcacta ataacactta 1200 cccgccggtg taatgaagac attgtattag gagaactatc tctaccagct ggtgttctag 1260 tctctttgcc attgattttg ttgcatcatg atgaagagat atggggtgaa gatgcaaagg 1320 agttcaaacc agagagattt agagaaggaa tatcaagtgc aacaaagggt caactcacat 1380 attttccatt tagctggggt cctagaatat gtattggaca aaattttgcc atgttagaag 1440 caaagatggc tctgtctatg atcctgcaac gcttctcttt tgaactgtct ccgtcttatg 1500 cacatgcccc tcggtccata ataaccgttc agcctcagta tggtgctcca cttattttcc 1560 acaaactata attttggtac ttctactaat attttagggt ttattcagac tcaaaaaaaa 1620 272 513 PRT NICOTIANATABACUM 272 Met Thr Val Thr Ala Ser Cys Ala Ala Ile Val Ile Thr Leu Leu Val 1 5 10 15 Cys Ile Trp Arg Val Leu Asn Trp Ile Trp Phe Arg Pro Lys Lys Leu 20 25 30 Glu Leu Leu Leu Arg Lys Gln Gly Leu Glu Gly Asn Ser Tyr Lys Val 35 40 45 Leu Tyr Gly Asp Met Lys Glu Phe Ser Gly Met Ile Lys Glu Ala Tyr 50 55 60 Ser Lys Pro Met Ser Leu Ser Asp Asp Val Ala Pro Arg Leu Met Pro 65 70 75 80 Phe Phe Leu Glu Thr Ile Lys Lys Tyr Gly Lys Arg Ser Phe Ile Trp 85 90 95 Phe Gly Pro Arg Pro Leu Val Leu Ile Met Asp Pro Glu Leu Ile Lys 100 105 110 Glu Val Leu Ser Lys Ile His Leu Tyr Gln Lys Pro Gly Gly Asn Pro 115 120 125 Leu Ala Thr Leu Leu Val Gln Gly Ile Ala Thr Tyr Glu Glu Asp Lys 130 135 140 Trp Ala Lys His Arg Lys Ile Ile Asn Pro Ala Phe His Leu Glu Lys 145 150 155 160 Leu Lys Leu Met Leu Pro Ala Phe Arg Leu Ser Cys Ser Glu Met Leu 165 170 175 Ser Lys Trp Glu Asp Ile Val Ser Ala Asp Ser Ser His Glu Ile Asp 180 185 190 Val Trp Ser His Leu Glu Gln Leu Thr Cys Asp Val Ile Ser Arg Thr 195 200 205 Ala Phe Gly Ser Ser Tyr Glu Glu Gly Arg Lys Ile Phe Glu Leu Gln 210 215 220 Lys Glu Gln Ala Gln Tyr Leu Val Glu Val Phe Arg Ser Val Tyr Ile 225 230 235 240 Pro Gly Arg Arg Phe Leu Pro Thr Lys Arg Asn Arg Arg Met Lys Glu 245 250 255 Ile Lys Lys Asp Val Arg Ala Ser Ile Lys Gly Ile Ile Asp Lys Arg 260 265 270 Leu Lys Ala Met Lys Ala Gly Asp Thr Asn Asn Glu Asp Leu Leu Gly 275 280 285 Ile Leu Leu Glu Ser Asn Ile Lys Glu Ile Glu Gln His Gly Asn Lys 290 295 300 Asp Phe Gly Met Ser Ile Glu Glu Val Ile Glu Glu Cys Lys Leu Phe 305 310 315 320 Tyr Phe Ala Gly Gln Glu Thr Thr Ser Val Leu Leu Leu Trp Ser Leu 325 330 335 Val Leu Leu Ser Arg Tyr Gln Asp Trp Gln Ala Arg Ala Arg Glu Glu 340 345 350 Ile Leu Gln Val Phe Gly Ser Arg Lys Pro Asp Phe Asp Gly Leu Asn 355 360 365 His Leu Lys Ile Val Thr Met Ile Leu Tyr Glu Ser Leu Arg Leu Tyr 370 375 380 Pro Ser Leu Ile Thr Leu Thr Arg Arg Cys Asn Glu Asp Ile Val Leu 385 390 395 400 Gly Glu Leu Ser Leu Pro Ala Gly Val Leu Val Ser Leu Pro Leu Ile 405 410 415 Leu Leu His His Asp Glu Glu Ile Trp Gly Glu Asp Ala Lys Glu Phe 420 425 430 Lys Pro Glu Arg Phe Arg Glu Gly Ile Ser Ser Ala Thr Lys Gly Gln 435 440 445 Leu Thr Tyr Phe Pro Phe Ser Trp Gly Pro Arg Ile Cys Ile Gly Gln 450 455 460 Asn Phe Ala Met Leu Glu Ala Lys Met Ala Leu Ser Met Ile Leu Gln 465 470 475 480 Arg Phe Ser Phe Glu Leu Ser Pro Ser Tyr Ala His Ala Pro Arg Ser 485 490 495 Ile Ile Thr Val Gln Pro Gln Tyr Gly Ala Pro Leu Ile Phe His Lys 500 505 510 Leu 273 1671 DNA NICOTIANATABACUM 273 tgcaatatag ttttcctagt cagttctagc ctccttttcc ttagaaataa tggattatca 60 tatttctttc cattttcaag ctcttttagg gcttttagcc tttgtgttct tgtctattat 120 cttatggaga agaacactca cttcaagaaa attagcccct gaaatcccag gggcatggcc 180 tattataggc catcttcgtc agctgagtgg tactgataag aatatcccat ttccccgaat 240 attgggcgct ttggcagata aatatggacc tgtcttcaca ctgagaatag ggatgtaccc 300 ctatttgatt gtcaacaatt gggaagcagc taaggattgt ctcacaacgc atgataagga 360 cttggctgcc cgaccaactt ctatggctgg tgaaagcatc gggtacaagt atgcgaggtt 420 tacttatgct aattttggtc cttattataa ccaagtgcgc aaactagccc tacaacatgt 480 actctcgagt actaaactcg agaaaatgaa acacatacgt gtttctgaat tggaaactag 540 catcaaagaa ttatattctt tgacgctggg caaaaacaac atgcaaaaag tgaatataag 600 taaatggttt gaacaattga ctttaaacat aatcgtgaag acaatttgtg gcaagagata 660 tagcaacata gaggaggatg aagaggcaca acgtttcaga aaggcattta agggcatcat 720 gtttgttgta gggcaaattg ttttatatga cgcaattcca ttcccattgt tcaaatactt 780 tgatttccaa ggtcatatac aattgatgaa caaaatttat aaagacttag attctattct 840 tcaaggatgg ttggatgatc atatgatgaa caaggatgta aacaataagg atcaagatgc 900 catagatgcc atgcttaagg taacacaact taatgaattc aaagcctatg gtttttctca 960 ggccactgtg atcaagtcga cagtcttgag tttgatctta gatggaaatg acacaaccgc 1020 tgttcatttg atatgggtaa tgtccttatt actgaacaat ccacatgtta tgaaacaagg 1080 ccaagaagag atagacatga aagtgggtaa agagaggtgg attgaagata ctgacataaa 1140 aaatttagtg taccttcagg ctatcgttaa agagacattg cgcttgtatc cacctgttcc 1200 ttttctttta ccacacgaag cagtgcaaga ttgtaaagtg actggttacc acattcctaa 1260 aggtactcgt ctatatatca atgcgtggaa agtacatcgc gatcctgaaa tttggtcaga 1320 gcccgaaaag tttatgccca atagattctt gactagcaaa gcaaatatag atgctcgcgg 1380 tcaaaatttt gaatttatac cgtttggttc tgggagacgg tcatgtccag ggataggttt 1440 tgcgacttta gtgacacatc tgacttttgg tcgcttgctt caaggttttg attttagtaa 1500 gccatcaaac acgccaattg acatgacaga aggcgtaggc gttactttgc ctaaggttaa 1560 tcaagttgaa gttctaatta cccctcgttt accttctaag ctttatttat tttgaaggtg 1620 caaatcatca atcatggctt gagtaattag ttatacttta atatgtttct c 1671 274 521 PRT NICOTIANATABACUM 274 Met Asp Tyr His Ile Ser Phe His Phe Gln Ala Leu Leu Gly Leu Leu 1 5 10 15 Ala Phe Val Phe Leu Ser Ile Ile Leu Trp Arg Arg Thr Leu Thr Ser 20 25 30 Arg Lys Leu Ala Pro Glu Ile Pro Gly Ala Trp Pro Ile Ile Gly His 35 40 45 Leu Arg Gln Leu Ser Gly Thr Asp Lys Asn Ile Pro Phe Pro Arg Ile 50 55 60 Leu Gly Ala Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Leu Arg Ile 65 70 75 80 Gly Met Tyr Pro Tyr Leu Ile Val Asn Asn Trp Glu Ala Ala Lys Asp 85 90 95 Cys Leu Thr Thr His Asp Lys Asp Leu Ala Ala Arg Pro Thr Ser Met 100 105 110 Ala Gly Glu Ser Ile Gly Tyr Lys Tyr Ala Arg Phe Thr Tyr Ala Asn 115 120 125 Phe Gly Pro Tyr Tyr Asn Gln Val Arg Lys Leu Ala Leu Gln His Val 130 135 140 Leu Ser Ser Thr Lys Leu Glu Lys Met Lys His Ile Arg Val Ser Glu 145 150 155 160 Leu Glu Thr Ser Ile Lys Glu Leu Tyr Ser Leu Thr Leu Gly Lys Asn 165 170 175 Asn Met Gln Lys Val Asn Ile Ser Lys Trp Phe Glu Gln Leu Thr Leu 180 185 190 Asn Ile Ile Val Lys Thr Ile Cys Gly Lys Arg Tyr Ser Asn Ile Glu 195 200 205 Glu Asp Glu Glu Ala Gln Arg Phe Arg Lys Ala Phe Lys Gly Ile Met 210 215 220 Phe Val Val Gly Gln Ile Val Leu Tyr Asp Ala Ile Pro Phe Pro Leu 225 230 235 240 Phe Lys Tyr Phe Asp Phe Gln Gly His Ile Gln Leu Met Asn Lys Ile 245 250 255 Tyr Lys Asp Leu Asp Ser Ile Leu Gln Gly Trp Leu Asp Asp His Met 260 265 270 Met Asn Lys Asp Val Asn Asn Lys Asp Gln Asp Ala Ile Asp Ala Met 275 280 285 Leu Lys Val Thr Gln Leu Asn Glu Phe Lys Ala Tyr Gly Phe Ser Gln 290 295 300 Ala Thr Val Ile Lys Ser Thr Val Leu Ser Leu Ile Leu Asp Gly Asn 305 310 315 320 Asp Thr Thr Ala Val His Leu Ile Trp Val Met Ser Leu Leu Leu Asn 325 330 335 Asn Pro His Val Met Lys Gln Gly Gln Glu Glu Ile Asp Met Lys Val 340 345 350 Gly Lys Glu Arg Trp Ile Glu Asp Thr Asp Ile Lys Asn Leu Val Tyr 355 360 365 Leu Gln Ala Ile Val Lys Glu Thr Leu Arg Leu Tyr Pro Pro Val Pro 370 375 380 Phe Leu Leu Pro His Glu Ala Val Gln Asp Cys Lys Val Thr Gly Tyr 385 390 395 400 His Ile Pro Lys Gly Thr Arg Leu Tyr Ile Asn Ala Trp Lys Val His 405 410 415 Arg Asp Pro Glu Ile Trp Ser Glu Pro Glu Lys Phe Met Pro Asn Arg 420 425 430 Phe Leu Thr Ser Lys Ala Asn Ile Asp Ala Arg Gly Gln Asn Phe Glu 435 440 445 Phe Ile Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Ile Gly Phe 450 455 460 Ala Thr Leu Val Thr His Leu Thr Phe Gly Arg Leu Leu Gln Gly Phe 465 470 475 480 Asp Phe Ser Lys Pro Ser Asn Thr Pro Ile Asp Met Thr Glu Gly Val 485 490 495 Gly Val Thr Leu Pro Lys Val Asn Gln Val Glu Val Leu Ile Thr Pro 500 505 510 Arg Leu Pro Ser Lys Leu Tyr Leu Phe 515 520 275 1618 DNA NICOTIANATABACUM 275 tttgattcaa ccatggagaa ccaatactcc tactcattct cttcctactt ctacttagct 60 atagtactgt ttcttcttcc aattttggtc aaatatttct tccatcggag aagaaattta 120 cctccaagtc cattttctct tccaataatt ggtcaccttt accttctcaa gaaaactctc 180 catctcactc taacatcctt atcagctaaa tatggtcctg ttttatacct caaattgggc 240 tctatgcctg tgattgttgt gtcctcacca tctgctgttg aagaatgttt aaccaagaat 300 gatatcatat tcgcaaatag gcccaagacc gtggctggtg acaagtttac ctacaattat 360 actgtttatg tttgggcacc ctatggccaa ctttggagaa ttcttcgccg attaactgtc 420 gttgaactct tctcttcaca tagcctacag aaaacttcta tccttagaga tcaagaagtt 480 gcaatattta tccgttcgtt atacaaattc tcaaaggata gtagcaaaaa agtcgatttg 540 accaactggt cttttacttt ggttttcaat cttatgacca aaattattgc tgggagacat 600 attgtgaagg aggaagatgc tggcaaggaa aagggcattg aaattattga aaaacttaga 660 gggactttct tagtaactac atcattcttg aatatgtgtg atttcttgcc agtattcagg 720 tgggttggtt acaaagggca ggagaagaag atggcctcaa ttcacaatag aagaaatgaa 780 ttcttgaaca gcttgcttga tgaatttcga cacaagaaaa gtagtgcttc acaatctaac 840 acaactgttg gaaacatgga gaagaaaacc acactgattg aaaagctctt gtctcttcaa 900 gaatcagagc ctgaattcta cactgatgat atcatcaaaa gtattatgct ggtagttttt 960 gttgcaggaa cagagacctc atcaacaacc atccaatggg taatgaggct tcttgtagct 1020 caccctgagg cattgtataa gctacgagct gacattgaca gtaaagttgg gaataagcgc 1080 ttgctgaatg aatcagacct caacaagctt ccgtatttgc attgtgttgt taatgagaca 1140 atgagattat acactccgat accactttta ttgcctcatt attcaactaa agattgtatt 1200 gtggaaggat atgatgtacc aaaacataca atgttgtttg tcaacgcttg ggccattcac 1260 agggatccca aggtatggga ggagcctgac aagttcaagc cagagagatt tgaggcaaca 1320 gaaggggaaa cagaaaggtt caattacaag cttgtaccat ttggaatggg gagaagagcg 1380 tgccctggag ctgatatggg gttgcgagca gtttctttgg cattaggtgc acttattcaa 1440 tgctttgact ggcaaattga ggaagcggaa agcttggagg aaagctataa ttctagaatg 1500 actatgcaga acaagccttt gaaggttgtc tgcactccac gcgaagatct tggccagctt 1560 ctatcccaac tctaaggcaa tttatcaatg ccaaacgtaa tcttcatcta ccactatg 1618 276 520 PRT NICOTIANATABACUM 276 Met Glu Asn Gln Tyr Ser Tyr Ser Phe Ser Ser Tyr Phe Tyr Leu Ala 1 5 10 15 Ile Val Leu Phe Leu Leu Pro Ile Leu Val Lys Tyr Phe Phe His Arg 20 25 30 Arg Arg Asn Leu Pro Pro Ser Pro Phe Ser Leu Pro Ile Ile Gly His 35 40 45 Leu Tyr Leu Leu Lys Lys Thr Leu His Leu Thr Leu Thr Ser Leu Ser 50 55 60 Ala Lys Tyr Gly Pro Val Leu Tyr Leu Lys Leu Gly Ser Met Pro Val 65 70 75 80 Ile Val Val Ser Ser Pro Ser Ala Val Glu Glu Cys Leu Thr Lys Asn 85 90 95 Asp Ile Ile Phe Ala Asn Arg Pro Lys Thr Val Ala Gly Asp Lys Phe 100 105 110 Thr Tyr Asn Tyr Thr Val Tyr Val Trp Ala Pro Tyr Gly Gln Leu Trp 115 120 125 Arg Ile Leu Arg Arg Leu Thr Val Val Glu Leu Phe Ser Ser His Ser 130 135 140 Leu Gln Lys Thr Ser Ile Leu Arg Asp Gln Glu Val Ala Ile Phe Ile 145 150 155 160 Arg Ser Leu Tyr Lys Phe Ser Lys Asp Ser Ser Lys Lys Val Asp Leu 165 170 175 Thr Asn Trp Ser Phe Thr Leu Val Phe Asn Leu Met Thr Lys Ile Ile 180 185 190 Ala Gly Arg His Ile Val Lys Glu Glu Asp Ala Gly Lys Glu Lys Gly 195 200 205 Ile Glu Ile Ile Glu Lys Leu Arg Gly Thr Phe Leu Val Thr Thr Ser 210 215 220 Phe Leu Asn Met Cys Asp Phe Leu Pro Val Phe Arg Trp Val Gly Tyr 225 230 235 240 Lys Gly Gln Glu Lys Lys Met Ala Ser Ile His Asn Arg Arg Asn Glu 245 250 255 Phe Leu Asn Ser Leu Leu Asp Glu Phe Arg His Lys Lys Ser Ser Ala 260 265 270 Ser Gln Ser Asn Thr Thr Val Gly Asn Met Glu Lys Lys Thr Thr Leu 275 280 285 Ile Glu Lys Leu Leu Ser Leu Gln Glu Ser Glu Pro Glu Phe Tyr Thr 290 295 300 Asp Asp Ile Ile Lys Ser Ile Met Leu Val Val Phe Val Ala Gly Thr 305 310 315 320 Glu Thr Ser Ser Thr Thr Ile Gln Trp Val Met Arg Leu Leu Val Ala 325 330 335 His Pro Glu Ala Leu Tyr Lys Leu Arg Ala Asp Ile Asp Ser Lys Val 340 345 350 Gly Asn Lys Arg Leu Leu Asn Glu Ser Asp Leu Asn Lys Leu Pro Tyr 355 360 365 Leu His Cys Val Val Asn Glu Thr Met Arg Leu Tyr Thr Pro Ile Pro 370 375 380 Leu Leu Leu Pro His Tyr Ser Thr Lys Asp Cys Ile Val Glu Gly Tyr 385 390 395 400 Asp Val Pro Lys His Thr Met Leu Phe Val Asn Ala Trp Ala Ile His 405 410 415 Arg Asp Pro Lys Val Trp Glu Glu Pro Asp Lys Phe Lys Pro Glu Arg 420 425 430 Phe Glu Ala Thr Glu Gly Glu Thr Glu Arg Phe Asn Tyr Lys Leu Val 435 440 445 Pro Phe Gly Met Gly Arg Arg Ala Cys Pro Gly Ala Asp Met Gly Leu 450 455 460 Arg Ala Val Ser Leu Ala Leu Gly Ala Leu Ile Gln Cys Phe Asp Trp 465 470 475 480 Gln Ile Glu Glu Ala Glu Ser Leu Glu Glu Ser Tyr Asn Ser Arg Met 485 490 495 Thr Met Gln Asn Lys Pro Leu Lys Val Val Cys Thr Pro Arg Glu Asp 500 505 510 Leu Gly Gln Leu Leu Ser Gln Leu 515 520 277 1676 DNA NICOTIANATABACUM 277 cctttttaag atgtatttaa gatttaagat ttaagatgaa gcaactgagg taagtccttt 60 caaggagtag ttgtcacttc tgagaatgga gatgatgtac agcataatag cagcagccag 120 tattgcaatt atcttggtat atacatggaa agtgttgaat tgggcttggt ttgggccgaa 180 gaaaatggag aaatgcttaa gacagagggg tctcaaggga aatccttata agctactcta 240 tggagatcta aacgaactga caaaaagcat aatagaagcc aagtctaagc ccatcaattt 300 ctctgatgat attgctcaaa ggctcatccc tttttttctt gacgccatca acaaaaatgg 360 taaaaactcc ttcgtctggc ttggaccgta tccaatagtg ttgatcacgg atcctgagca 420 tttaaaggag attttcacaa agaattatgt gtatcaaaag caaactcatc ccaatccata 480 cgccaagcta ttagctcacg gtcttgtcag ccttgaggaa gacaaatggg ccaaacacag 540 aaaaatcatt agtcctgcct tccatgtcga gaagctaaag catatgctgc ctgcatttta 600 tctgagttgt agtgaaatga taagcaaatg ggaggaggtt gttccaaaag aaacatcatt 660 cgagctcgat gtatggccag accttcaaat aatgaccagt gaagtcattt ctcgcactgc 720 atttgggagt agctatgaag aaggaagaat agtatttgaa cttcagaaag aacaagctga 780 gtatgtaatg gacataggac gttcaattta tataccagga tcaaggttct tgcctactaa 840 aaggaacaaa agaatgctgg aaattgaaaa gcaagtgcaa acaacaatta ggcgtatcat 900 cgacaaaaga ttgaaggcaa tggaagaagg ggagactagt aaagatgact tattaggcat 960 attacttgaa tccaatttga aagaaattga acttcatgga agaaatgact tgggaataac 1020 aacgtcagaa gtgattgaag agtgcaagtt attctatttt gccggccaag agaccacttc 1080 agtgttgctt gtttggacaa tgattttgtt gtgcttacat ccagagtggc aagtacgtgc 1140 cagaaaggaa gtgttgcaga tctttggaaa tgataaacca gatttggaag gactaagtcg 1200 cttgaaaatt gtaacaatga tcttgtacga gacgttacgc ctattccccc cattaccagc 1260 atttggtaga aggaacaaag aagaagtcaa attaggggag ctacatctac cggctggagt 1320 gttactcgtt ataccagcaa tcttagtaca ttatgataag gaaatatggg gtgaagatgc 1380 aaaggaattc aaaccagaaa gattcagtga aggagtgtca aaggcaacaa atggacaagt 1440 ctcatttata ccatttagct ggggacctcg tgtttgcatt ggacaaaact tcgcaatgat 1500 ggaagcaaaa atggcagtaa ctatgatact acaaaaattc tcctttgaac tatccccttc 1560 ttatacacat gctccatttg caattgtgac tattcatccc cagtatggtg ctcctctgct 1620 tatgcgcaga ctttaaaaca tatgttgctg atatttaaga tcagtggcgt tttatt 1676 278 516 PRT NICOTIANATABACUM 278 Met Glu Met Met Tyr Ser Ile Ile Ala Ala Ala Ser Ile Ala Ile Ile 1 5 10 15 Leu Val Tyr Thr Trp Lys Val Leu Asn Trp Ala Trp Phe Gly Pro Lys 20 25 30 Lys Met Glu Lys Cys Leu Arg Gln Arg Gly Leu Lys Gly Asn Pro Tyr 35 40 45 Lys Leu Leu Tyr Gly Asp Leu Asn Glu Leu Thr Lys Ser Ile Ile Glu 50 55 60 Ala Lys Ser Lys Pro Ile Asn Phe Ser Asp Asp Ile Ala Gln Arg Leu 65 70 75 80 Ile Pro Phe Phe Leu Asp Ala Ile Asn Lys Asn Gly Lys Asn Ser Phe 85 90 95 Val Trp Leu Gly Pro Tyr Pro Ile Val Leu Ile Thr Asp Pro Glu His 100 105 110 Leu Lys Glu Ile Phe Thr Lys Asn Tyr Val Tyr Gln Lys Gln Thr His 115 120 125 Pro Asn Pro Tyr Ala Lys Leu Leu Ala His Gly Leu Val Ser Leu Glu 130 135 140 Glu Asp Lys Trp Ala Lys His Arg Lys Ile Ile Ser Pro Ala Phe His 145 150 155 160 Val Glu Lys Leu Lys His Met Leu Pro Ala Phe Tyr Leu Ser Cys Ser 165 170 175 Glu Met Ile Ser Lys Trp Glu Glu Val Val Pro Lys Glu Thr Ser Phe 180 185 190 Glu Leu Asp Val Trp Pro Asp Leu Gln Ile Met Thr Ser Glu Val Ile 195 200 205 Ser Arg Thr Ala Phe Gly Ser Ser Tyr Glu Glu Gly Arg Ile Val Phe 210 215 220 Glu Leu Gln Lys Glu Gln Ala Glu Tyr Val Met Asp Ile Gly Arg Ser 225 230 235 240 Ile Tyr Ile Pro Gly Ser Arg Phe Leu Pro Thr Lys Arg Asn Lys Arg 245 250 255 Met Leu Glu Ile Glu Lys Gln Val Gln Thr Thr Ile Arg Arg Ile Ile 260 265 270 Asp Lys Arg Leu Lys Ala Met Glu Glu Gly Glu Thr Ser Lys Asp Asp 275 280 285 Leu Leu Gly Ile Leu Leu Glu Ser Asn Leu Lys Glu Ile Glu Leu His 290 295 300 Gly Arg Asn Asp Leu Gly Ile Thr Thr Ser Glu Val Ile Glu Glu Cys 305 310 315 320 Lys Leu Phe Tyr Phe Ala Gly Gln Glu Thr Thr Ser Val Leu Leu Val 325 330 335 Trp Thr Met Ile Leu Leu Cys Leu His Pro Glu Trp Gln Val Arg Ala 340 345 350 Arg Lys Glu Val Leu Gln Ile Phe Gly Asn Asp Lys Pro Asp Leu Glu 355 360 365 Gly Leu Ser Arg Leu Lys Ile Val Thr Met Ile Leu Tyr Glu Thr Leu 370 375 380 Arg Leu Phe Pro Pro Leu Pro Ala Phe Gly Arg Arg Asn Lys Glu Glu 385 390 395 400 Val Lys Leu Gly Glu Leu His Leu Pro Ala Gly Val Leu Leu Val Ile 405 410 415 Pro Ala Ile Leu Val His Tyr Asp Lys Glu Ile Trp Gly Glu Asp Ala 420 425 430 Lys Glu Phe Lys Pro Glu Arg Phe Ser Glu Gly Val Ser Lys Ala Thr 435 440 445 Asn Gly Gln Val Ser Phe Ile Pro Phe Ser Trp Gly Pro Arg Val Cys 450 455 460 Ile Gly Gln Asn Phe Ala Met Met Glu Ala Lys Met Ala Val Thr Met 465 470 475 480 Ile Leu Gln Lys Phe Ser Phe Glu Leu Ser Pro Ser Tyr Thr His Ala 485 490 495 Pro Phe Ala Ile Val Thr Ile His Pro Gln Tyr Gly Ala Pro Leu Leu 500 505 510 Met Arg Arg Leu 515 279 1577 DNA NICOTIANATABACUM 279 ctaaatttca tataccttta gtactcttga aattttcaaa taatggttta tcttctttct 60 cccatagaag ccattgtagg atttgtaacc ttttcatttc tattctactt tctatggacc 120 aaaaaacaat caaaaatctt aaacccacta cctccaaaaa tcccaggtgg atggccagta 180 atcggccatc tcttttattt caagaacaat ggcgatgaag atcgccattt ttctcaaaaa 240 ctcggtgact tagctgacaa atatggtccc gtcttcactt tccggttagg gtttcgccgt 300 ttcttggcgg tgagtagtta tgaagctatg aaagaatgct tcactaccaa tgatatccat 360 ttcgccgatc ggccatcttt actctacgga gaataccttt gctataataa tgccatgctt 420 gctgttgcca aatatggccc ttactggaaa aaaaatcgaa agttagtcaa tcaagaagtt 480 ctctccgtta gtcggctcga aaaattcaaa catgttagat tttctataat tcagaaaaat 540 attaaacaat tgtataattg tgattcacca atggtgaaga taaaccttag tgattggata 600 gataaattga cattcgacat cattttgaaa atggttgttg ggaagaacta taataatgga 660 catggagaaa tactcaaagt tgcttttcag aaattcatgg ttcaagctat ggagatggag 720 ctctatgatg tttttcacat tccatttttc aagtggttgg atcttacagg gaatattaag 780 gctatgaaac aaactttcaa agacattgat aatattatcc aaggttggtt agatgagcac 840 attaagaaga gagaaacaaa ggatgttgga ggtgaaaacg aacaagattt tatagatgtg 900 gtgctttcca agatgagcga cgaacatctt ggcgagggtt actctcatga cacaaccatc 960 aaagcaactg tattcacttt ggtcttggat gcaacagaca cacttgcact tcatataaag 1020 tgggtaatgg cgttaatgat aaacaataag catgtcatga agaaagcaca agaagagatg 1080 gacacaattg ttggtagaga tagatgggta gaagagagtg atatcaagaa tttggtgtat 1140 ctccaagcaa ttgttaaaga agtattacga ttacatccac ctgcaccttt gtcagtgcaa 1200 cacctatctg tggaagattg tgttgtcaat gggtaccata ttcctaaggg gactgcacta 1260 cttaccaata ttatgaaact acagcgagat cctcaaacat ggccaaatcc tgataaattc 1320 gatccagaga gattcctgac gactcatgct actattgact accgcgggca gcactatgag 1380 ttgatcccct ttggtacggg gagacgagct tgtcccgcga tgaattattc attgcaagtg 1440 gaacaccttt caattgctca tatgatccaa ggtttcagtt ttgcaactac gaccaatgag 1500 cctttggata tgaaacaagg tgtgggttta actttaccaa agaagactga tgttgaagtt 1560 ctaattaccc ctcgttt 1577 280 511 PRT NICOTIANATABACUM 280 Met Val Tyr Leu Leu Ser Pro Ile Glu Ala Ile Val Gly Phe Val Thr 1 5 10 15 Phe Ser Phe Leu Phe Tyr Phe Leu Trp Thr Lys Lys Gln Ser Lys Ile 20 25 30 Leu Asn Pro Leu Pro Pro Lys Ile Pro Gly Gly Trp Pro Val Ile Gly 35 40 45 His Leu Phe Tyr Phe Lys Asn Asn Gly Asp Glu Asp Arg His Phe Ser 50 55 60 Gln Lys Leu Gly Asp Leu Ala Asp Lys Tyr Gly Pro Val Phe Thr Phe 65 70 75 80 Arg Leu Gly Phe Arg Arg Phe Leu Ala Val Ser Ser Tyr Glu Ala Met 85 90 95 Lys Glu Cys Phe Thr Thr Asn Asp Ile His Phe Ala Asp Arg Pro Ser 100 105 110 Leu Leu Tyr Gly Glu Tyr Leu Cys Tyr Asn Asn Ala Met Leu Ala Val 115 120 125 Ala Lys Tyr Gly Pro Tyr Trp Lys Lys Asn Arg Lys Leu Val Asn Gln 130 135 140 Glu Val Leu Ser Val Ser Arg Leu Glu Lys Phe Lys His Val Arg Phe 145 150 155 160 Ser Ile Ile Gln Lys Asn Ile Lys Gln Leu Tyr Asn Cys Asp Ser Pro 165 170 175 Met Val Lys Ile Asn Leu Ser Asp Trp Ile Asp Lys Leu Thr Phe Asp 180 185 190 Ile Ile Leu Lys Met Val Val Gly Lys Asn Tyr Asn Asn Gly His Gly 195 200 205 Glu Ile Leu Lys Val Ala Phe Gln Lys Phe Met Val Gln Ala Met Glu 210 215 220 Met Glu Leu Tyr Asp Val Phe His Ile Pro Phe Phe Lys Trp Leu Asp 225 230 235 240 Leu Thr Gly Asn Ile Lys Ala Met Lys Gln Thr Phe Lys Asp Ile Asp 245 250 255 Asn Ile Ile Gln Gly Trp Leu Asp Glu His Ile Lys Lys Arg Glu Thr 260 265 270 Lys Asp Val Gly Gly Glu Asn Glu Gln Asp Phe Ile Asp Val Val Leu 275 280 285 Ser Lys Met Ser Asp Glu His Leu Gly Glu Gly Tyr Ser His Asp Thr 290 295 300 Thr Ile Lys Ala Thr Val Phe Thr Leu Val Leu Asp Ala Thr Asp Thr 305 310 315 320 Leu Ala Leu His Ile Lys Trp Val Met Ala Leu Met Ile Asn Asn Lys 325 330 335 His Val Met Lys Lys Ala Gln Glu Glu Met Asp Thr Ile Val Gly Arg 340 345 350 Asp Arg Trp Val Glu Glu Ser Asp Ile Lys Asn Leu Val Tyr Leu Gln 355 360 365 Ala Ile Val Lys Glu Val Leu Arg Leu His Pro Pro Ala Pro Leu Ser 370 375 380 Val Gln His Leu Ser Val Glu Asp Cys Val Val Asn Gly Tyr His Ile 385 390 395 400 Pro Lys Gly Thr Ala Leu Leu Thr Asn Ile Met Lys Leu Gln Arg Asp 405 410 415 Pro Gln Thr Trp Pro Asn Pro Asp Lys Phe Asp Pro Glu Arg Phe Leu 420 425 430 Thr Thr His Ala Thr Ile Asp Tyr Arg Gly Gln His Tyr Glu Leu Ile 435 440 445 Pro Phe Gly Thr Gly Arg Arg Ala Cys Pro Ala Met Asn Tyr Ser Leu 450 455 460 Gln Val Glu His Leu Ser Ile Ala His Met Ile Gln Gly Phe Ser Phe 465 470 475 480 Ala Thr Thr Thr Asn Glu Pro Leu Asp Met Lys Gln Gly Val Gly Leu 485 490 495 Thr Leu Pro Lys Lys Thr Asp Val Glu Val Leu Ile Thr Pro Arg 500 505 510 281 1434 DNA NICOTIANATABACUM misc_feature (564)..(564) n is a, c, g, or t 281 ctttttctcc ccaaaaaaga gctcatttcc cttgtcccca aaaatggatc ttctcttact 60 agagaagacc ttaattggtc tcttctttgc cattttaatc gctgtaattg tctctagact 120 tcgttcaaag cgttttaagc ttcccccagg accaatccca gtaccagttt ttggtaattg 180 gcttcaagtt ggtgatgatt taaaccacag aaatcttact gattttgcca aaaaatttgg 240 tgatcttttc ttgttaagaa tgggccagcg taatttagtt gttgtgtcat ctcctgaatt 300 agctaaagaa gttttacaca cacaaggtgt tgaatttggt tcaagaacaa gaaatgttgt 360 atttgatatt tttactggaa aaggtcaaga tatggttttt actgtatatg gtgaacactg 420 gagaaaaatg aggagaatta tgactgtacc attttttact aataaagttg tgcagcaata 480 tagagggggg tgggagtttg aagtggcaag tgtaattgag gatgtgaaga aaaatcctga 540 atctgctact aatgggattg tatnaaggag gagattacaa ttgatgatgt ataataatat 600 gtttaggatt atgtttgata ggagatttga gagtgaagat gatcctttgt ttgttaagct 660 taaggctttg aatggtgaaa ggagtagatt ggctcagagt tttgagtata attatggtga 720 ttttattccc attttgaggc cttttttgag aggttatttg aagatctgta aagaagttaa 780 ggagaagagg ctgcagcttt tcaaagatta ctttgttgat gaaagaaaga agctttcaaa 840 taccaagagc ttggacagca atgctctgaa atgtgcgatt gatcacattc ttgaggctca 900 acagaagggg gagatcaatg aggacaacgt tctttacatt gttgaaaaca tcaatgttgc 960 tgctatagaa accacattat ggtcaattga gtggggtatc gccgagttag tcaaccaccc 1020 tcacatccaa aagaaactcc gcgacgagat tgacacagtt cttggcccag gagtgcaagt 1080 gactgaacca gacacccaca agcttccata ccttcaggct gtgatcaagg agacgcttcg 1140 tctccgtatg gcaattcctc tattagtccc acacatgaac cttcacgatg caaagcttgg 1200 cgggtttgat attccagcag agagcaaaat cttggttaac gcttggtggc tagctaacaa 1260 cccggctcat tggaagaaac ccgaagagtt cagacccgag aggttcttcg aagaggagaa 1320 gcacgttgag gccaatggca atgacttcag atatcttccg tttggcgttg gtaggaggag 1380 ttgccctgga actatacttg cattgccaat tcttggcatt actttgggac gttt 1434 282 463 PRT NICOTIANATABACUM 282 Met Asp Leu Leu Leu Leu Glu Lys Thr Leu Ile Gly Leu Phe Phe Ala 1 5 10 15 Ile Leu Ile Ala Val Ile Val Ser Arg Leu Arg Ser Lys Arg Phe Lys 20 25 30 Leu Pro Pro Gly Pro Ile Pro Val Pro Val Phe Gly Asn Trp Leu Gln 35 40 45 Val Gly Asp Asp Leu Asn His Arg Asn Leu Thr Asp Phe Ala Lys Lys 50 55 60 Phe Gly Asp Leu Phe Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val 65 70 75 80 Val Ser Ser Pro Glu Leu Ala Lys Glu Val Leu His Thr Gln Gly Val 85 90 95 Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly 100 105 110 Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp Arg Lys 115 120 125 Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln 130 135 140 Gln Tyr Arg Gly Gly Trp Glu Phe Glu Val Ala Ser Val Ile Glu Asp 145 150 155 160 Val Lys Lys Asn Pro Glu Ser Ala Thr Asn Gly Ile Val Leu Arg Arg 165 170 175 Arg Leu Gln Leu Met Met Tyr Asn Asn Met Phe Arg Ile Met Phe Asp 180 185 190 Arg Arg Phe Glu Ser Glu Asp Asp Pro Leu Phe Val Lys Leu Lys Ala 195 200 205 Leu Asn Gly Glu Arg Ser Arg Leu Ala Gln Ser Phe Glu Tyr Asn Tyr 210 215 220 Gly Asp Phe Ile Pro Ile Leu Arg Pro Phe Leu Arg Gly Tyr Leu Lys 225 230 235 240 Ile Cys Lys Glu Val Lys Glu Lys Arg Leu Gln Leu Phe Lys Asp Tyr 245 250 255 Phe Val Asp Glu Arg Lys Lys Leu Ser Asn Thr Lys Ser Leu Asp Ser 260 265 270 Asn Ala Leu Lys Cys Ala Ile Asp His Ile Leu Glu Ala Gln Gln Lys 275 280 285 Gly Glu Ile Asn Glu Asp Asn Val Leu Tyr Ile Val Glu Asn Ile Asn 290 295 300 Val Ala Ala Ile Glu Thr Thr Leu Trp Ser Ile Glu Trp Gly Ile Ala 305 310 315 320 Glu Leu Val Asn His Pro His Ile Gln Lys Lys Leu Arg Asp Glu Ile 325 330 335 Asp Thr Val Leu Gly Pro Gly Val Gln Val Thr Glu Pro Asp Thr His 340 345 350 Lys Leu Pro Tyr Leu Gln Ala Val Ile Lys Glu Thr Leu Arg Leu Arg 355 360 365 Met Ala Ile Pro Leu Leu Val Pro His Met Asn Leu His Asp Ala Lys 370 375 380 Leu Gly Gly Phe Asp Ile Pro Ala Glu Ser Lys Ile Leu Val Asn Ala 385 390 395 400 Trp Trp Leu Ala Asn Asn Pro Ala His Trp Lys Lys Pro Glu Glu Phe 405 410 415 Arg Pro Glu Arg Phe Phe Glu Glu Glu Lys His Val Glu Ala Asn Gly 420 425 430 Asn Asp Phe Arg Tyr Leu Pro Phe Gly Val Gly Arg Arg Ser Cys Pro 435 440 445 Gly Thr Ile Leu Ala Leu Pro Ile Leu Gly Ile Thr Leu Gly Arg 450 455 460 283 1723 DNA NICOTIANATABACUM 283 ccaaattaga gcaagaaatt aacaagtcta gttaccttct ccctttttaa gagtatttaa 60 gatttaagat ttaagatgaa gcaactgagg taagtccttt caaggagtag ttgtcacttc 120 tgagaatgga gatgatgtac agcataatag cagcagccag tattgcaatt atcttggtat 180 atacatggaa agtgttgaat tgggcttggt ttgggccaaa gaaaatggag aaatgcttaa 240 gacagagggg tctcaaggga aatccttata agctactcta tggagatcta aacgaactga 300 caaaaagcat aatagaagcc aagtctaagc ccatcaattt ctctgatgat attgctcaaa 360 ggctcatccc tttttttctt gacgccatca acaaaaatgg taaaaactcc ttcgtctggc 420 ttggaccgta tccaatagtg ttgatcacgg atcctgagca tttaaaggag attttcacaa 480 agaattatgt gtatcaaaag caaactcatc ccaatccata cgccaagcta ttagctcacg 540 gtcttgtcag ccttgaggaa gacaaatggg ccaaacacag aaaaatcatt agtcctgcct 600 tccatgtcga gaagctaaag catatgctgc ctgcatttta tctgagttgt agtgaaatga 660 taagcaaatg ggaggaggtt gttccaaaag aaacatcatt cgagctcgat gtatggccag 720 accttcaaat aatgaccagt gaagtcattt ctcgcactgc atttgggagt agctatgaag 780 aaggaagaat agtatttgaa cttcagaaag aacaagctga gtatgtaatg gacataggac 840 gttcaattta tataccagga tcaaggttct tgcctactaa aaggaacaaa agaatgctgg 900 aaattgaaaa gcaagtgcaa acaacaatta ggcgtatcat cgacaaaaga ttgaaggcaa 960 tggaagaagg ggagactagt aaagatgact tattaggcat attacttgaa tccaatttga 1020 aagaaattga acttcatgga agaaatgact tgggaataac aacatcagaa gtgattgaag 1080 agtgcaagtt aatctatttt gccggccaag agaccacttc agtgttgctt gtttggacaa 1140 tgattttgtt gtgcttacat ccagagtggc aagtacgtgc cagaaaggaa gtgttgcaga 1200 cctttggaaa tgataaacca gatttggaag gactaagtcg cttgaaaatt gtaacaatga 1260 tcttgtacga gacgttacgc ctattccccc cattaccagc atttggtaga aggaacaaag 1320 aagaagtcaa attaggggag ctacatctac cggctggagt gttactcgtt ataccagcaa 1380 tcttagtaca ttatgataag gaaatatggg gtgaagatgc aaaggaattc aaaccagaaa 1440 gattcagtga aggagtgtca aaggcaacaa atggacaagt ctcatttata ccatttagct 1500 agggacctcg tgtttgcatt ggacaaaact tcgcaatgat ggaagcaaaa atggcagtaa 1560 ctatgatact acaaaaattc tcctttgaac tatccccttc ttatacacat gctccatttg 1620 caattgtgac tattcatccc cagtatggtg ctcctctgct tatgcgcaga ctttaaaaca 1680 tatgttgctg atatttaaga tcagtggcgt tttattctcc atg 1723 284 458 PRT NICOTIANATABACUM 284 Met Glu Met Met Tyr Ser Ile Ile Ala Ala Ala Ser Ile Ala Ile Ile 1 5 10 15 Leu Val Tyr Thr Trp Lys Val Leu Asn Trp Ala Trp Phe Gly Pro Lys 20 25 30 Lys Met Glu Lys Cys Leu Arg Gln Arg Gly Leu Lys Gly Asn Pro Tyr 35 40 45 Lys Leu Leu Tyr Gly Asp Leu Asn Glu Leu Thr Lys Ser Ile Ile Glu 50 55 60 Ala Lys Ser Lys Pro Ile Asn Phe Ser Asp Asp Ile Ala Gln Arg Leu 65 70 75 80 Ile Pro Phe Phe Leu Asp Ala Ile Asn Lys Asn Gly Lys Asn Ser Phe 85 90 95 Val Trp Leu Gly Pro Tyr Pro Ile Val Leu Ile Thr Asp Pro Glu His 100 105 110 Leu Lys Glu Ile Phe Thr Lys Asn Tyr Val Tyr Gln Lys Gln Thr His 115 120 125 Pro Asn Pro Tyr Ala Lys Leu Leu Ala His Gly Leu Val Ser Leu Glu 130 135 140 Glu Asp Lys Trp Ala Lys His Arg Lys Ile Ile Ser Pro Ala Phe His 145 150 155 160 Val Glu Lys Leu Lys His Met Leu Pro Ala Phe Tyr Leu Ser Cys Ser 165 170 175 Glu Met Ile Ser Lys Trp Glu Glu Val Val Pro Lys Glu Thr Ser Phe 180 185 190 Glu Leu Asp Val Trp Pro Asp Leu Gln Ile Met Thr Ser Glu Val Ile 195 200 205 Ser Arg Thr Ala Phe Gly Ser Ser Tyr Glu Glu Gly Arg Ile Val Phe 210 215 220 Glu Leu Gln Lys Glu Gln Ala Glu Tyr Val Met Asp Ile Gly Arg Ser 225 230 235 240 Ile Tyr Ile Pro Gly Ser Arg Phe Leu Pro Thr Lys Arg Asn Lys Arg 245 250 255 Met Leu Glu Ile Glu Lys Gln Val Gln Thr Thr Ile Arg Arg Ile Ile 260 265 270 Asp Lys Arg Leu Lys Ala Met Glu Glu Gly Glu Thr Ser Lys Asp Asp 275 280 285 Leu Leu Gly Ile Leu Leu Glu Ser Asn Leu Lys Glu Ile Glu Leu His 290 295 300 Gly Arg Asn Asp Leu Gly Ile Thr Thr Ser Glu Val Ile Glu Glu Cys 305 310 315 320 Lys Leu Ile Tyr Phe Ala Gly Gln Glu Thr Thr Ser Val Leu Leu Val 325 330 335 Trp Thr Met Ile Leu Leu Cys Leu His Pro Glu Trp Gln Val Arg Ala 340 345 350 Arg Lys Glu Val Leu Gln Thr Phe Gly Asn Asp Lys Pro Asp Leu Glu 355 360 365 Gly Leu Ser Arg Leu Lys Ile Val Thr Met Ile Leu Tyr Glu Thr Leu 370 375 380 Arg Leu Phe Pro Pro Leu Pro Ala Phe Gly Arg Arg Asn Lys Glu Glu 385 390 395 400 Val Lys Leu Gly Glu Leu His Leu Pro Ala Gly Val Leu Leu Val Ile 405 410 415 Pro Ala Ile Leu Val His Tyr Asp Lys Glu Ile Trp Gly Glu Asp Ala 420 425 430 Lys Glu Phe Lys Pro Glu Arg Phe Ser Glu Gly Val Ser Lys Ala Thr 435 440 445 Asn Gly Gln Val Ser Phe Ile Pro Phe Ser 450 455 285 1717 DNA NICOTIANATABACUM 285 tttgcctttg ctcgtcattg atgacgactt cattttgttt tcttccccac gaaaatggta 60 gatatgatat ggagggacgt agggaagaat tactgggaca aacctagtga gtgaaaatgg 120 aaacagttga aatgatagta aaagtatctt gtgctgccat agtaattact ctgttggtgt 180 gtctatggag agtgctgaat tgggtttggt tcagaccaaa gaaattagag aagttgttga 240 gaaaacaggt tttgtatggg gacatgaaag agttttctgg gatgattaag gaagcatact 300 caaagcctat gagtctgtct gatgatgtag caccacgaat gatgcctttc tttcttgaaa 360 ccatcaagaa atatggaaaa agatccttta tatggttcgg tccaagacca ctagtattga 420 tcatggatcc tgagcttata aaggaagtac tctccaaaat ctatctttat caaaagcccg 480 gtggaaatcc attagcaaca ctattggtac aaggattagc aacctatgag gaagacaaat 540 gggccaaaca tagaaaaatc atcaatcccg ctttccatct agagaagcta aagcatatgc 600 ttccagcttt tcgcttgagc tgtagtgaga tgctgagcaa atgggaagac attgtttcag 660 ctgaaggctc acatgagata gatgtatggc ctaaccttga gcaattgagt tgcgatgtga 720 tctctcggac agcttttggc aatagttatg aagaaggtag aaagattttt gaacttcaaa 780 aggaacaaac tcagcatctt gtggaagctt tccgctctgt ttatatccca ggaaggagat 840 ttttgccaac aaagaggaat agaagaatga aggaaataaa aaaggaggtt cgagcgtcaa 900 ttaaaggtat tattgataaa agattgaagg caatgaaagc aggggacacc aataatgagg 960 atctattggg atattgctgg aatcaaattt taaagaaatt gaacagcgcg gaaacaagga 1020 ttttggaatg agcattgaag atgtcattga agaatgcaag ttattctatt ttgctggcca 1080 agaaactaca tcagtgttgc tcctatggtc tctagtgtcg ttgagcaggt atcaagattg 1140 gcagacacgg gccagagaag aagtcttgca tgtctttggg agtcggaaac cagattttga 1200 tgaattaaat catctaaaag ttgtgacaat gatcatgtac gagtctttaa ggctatatcc 1260 ctcactaata acacttaccc gccggtgtaa tgaagacatt gtattaggag aactatctct 1320 accagctggt gtcctagtct ctttgccaat gattttgttg catcatgatg aagagatatg 1380 gggtgaagat gcaaaggagt tcaaaccaga gagatttaga gaaggattgt caagtgcaac 1440 aaagggtcaa cttacatatt ttccatttgg ctggggtcct agaatatgta ttggacaaaa 1500 ttttgccatg ttagaagcaa agatggctct gtctatgatc ctgcaacgct tctcttttga 1560 actgtctccg tcttatgcac atgcccctca gtccatatta accgttcagc ctcaatatgg 1620 tgctccactt attttccaca agctataatt tggtacttgt gaaaggtgtc ttgtacaata 1680 tgttagtaga gtttattcag acttagatac atgcttc 1717 286 304 PRT NICOTIANATABACUM 286 Met Glu Thr Val Glu Met Ile Val Lys Val Ser Cys Ala Ala Ile Val 1 5 10 15 Ile Thr Leu Leu Val Cys Leu Trp Arg Val Leu Asn Trp Val Trp Phe 20 25 30 Arg Pro Lys Lys Leu Glu Lys Leu Leu Arg Lys Gln Val Leu Tyr Gly 35 40 45 Asp Met Lys Glu Phe Ser Gly Met Ile Lys Glu Ala Tyr Ser Lys Pro 50 55 60 Met Ser Leu Ser Asp Asp Val Ala Pro Arg Met Met Pro Phe Phe Leu 65 70 75 80 Glu Thr Ile Lys Lys Tyr Gly Lys Arg Ser Phe Ile Trp Phe Gly Pro 85 90 95 Arg Pro Leu Val Leu Ile Met Asp Pro Glu Leu Ile Lys Glu Val Leu 100 105 110 Ser Lys Ile Tyr Leu Tyr Gln Lys Pro Gly Gly Asn Pro Leu Ala Thr 115 120 125 Leu Leu Val Gln Gly Leu Ala Thr Tyr Glu Glu Asp Lys Trp Ala Lys 130 135 140 His Arg Lys Ile Ile Asn Pro Ala Phe His Leu Glu Lys Leu Lys His 145 150 155 160 Met Leu Pro Ala Phe Arg Leu Ser Cys Ser Glu Met Leu Ser Lys Trp 165 170 175 Glu Asp Ile Val Ser Ala Glu Gly Ser His Glu Ile Asp Val Trp Pro 180 185 190 Asn Leu Glu Gln Leu Ser Cys Asp Val Ile Ser Arg Thr Ala Phe Gly 195 200 205 Asn Ser Tyr Glu Glu Gly Arg Lys Ile Phe Glu Leu Gln Lys Glu Gln 210 215 220 Thr Gln His Leu Val Glu Ala Phe Arg Ser Val Tyr Ile Pro Gly Arg 225 230 235 240 Arg Phe Leu Pro Thr Lys Arg Asn Arg Arg Met Lys Glu Ile Lys Lys 245 250 255 Glu Val Arg Ala Ser Ile Lys Gly Ile Ile Asp Lys Arg Leu Lys Ala 260 265 270 Met Lys Ala Gly Asp Thr Asn Asn Glu Asp Leu Leu Gly Tyr Cys Trp 275 280 285 Asn Gln Ile Leu Lys Lys Leu Asn Ser Ala Glu Thr Arg Ile Leu Glu 290 295 300 287 1559 DNA NICOTIANATABACUM 287 ccaaaatgca gttcttcaac ttcatttcct ttgtcttttt tgtgtctttc ctctttttat 60 taaggaaatg gaagaactcc aatagccaaa ccaaaagatt gcctccaggt ccatggaaat 120 tacctgtact tggaagcatg tttcatttgc taggtggacc tccacatcat gtccttggag 180 atttagccaa aaaatatggt ccacttatgc accttcaact aggtgaagtt tctgtagttt 240 ctgttacttc tcctgagatg gcaaaagaag tactaaaaac tcatgacctc gcttttgcat 300 ctaggccgtt acttttggca gccaaaattg tctgctataa tgggacagac attgtctttt 360 ccccctatgg cgattattgg agacaaacgc gtaaaatttg tctcttggaa ttgctcagtg 420 ccaaaaatgt taggtcattc agctcagtca gacgagatga agttttccat atgattgaat 480 ttttttcgat catcttctgg taagccagtt aatgtatcaa aaaggatttc tctattcaca 540 acctctatga catgtagatc agcctttgga caagaataca aggagcaaga cgaattcgca 600 caactagtaa aaaaagtgtc aagcttaatg gaagggtttg atgttgctga tatattccct 660 tcattgaagt ttcttcatgt gctcagtgga atgaaggcta aagttatgga tgcacaccat 720 gagttagatg ccattcttga aaaaattatc aatgagcaca agaaaattgc aactggaaag 780 aataataatg aattaggagg tgaaggatta attgacgtac tgctaagact tatgaaagag 840 ggaggccttc aattcccgat caccaacgac aacatcaaag ctattatttt tgacatgttt 900 ggtgcgggaa cggaaacttc atcaaccaca attgactggg ccatggtcga aatgataaag 960 aatccaagtg tattcgctaa agctcaagca gaggtaagag aagccttcag agagaaagaa 1020 acttttgatg aaaatgatgt cgaggagttg aaatacttaa aattggttat caaagaaact 1080 ttcagactcc atcctccatt tccccttttg ctcccaagag aatctagaga agaaacagat 1140 ataaacggct acactattcc ttttaaaaca aaacttatgg ttaacgttcg ggctattgga 1200 agagatccaa aatattggga tgacgtggaa agttttaagc cagagagatt tgagcacaac 1260 tctatggatt ttattggtaa taattttgaa tatcttccct ttggtagtgg aaggagaatg 1320 tgccctggga tatcatttgg tttggctaat gtttatttgc cactagctca attgttatat 1380 cattttgatt ggaaactccc tactggaatc aattcaagtg acttggacat gactgagtcg 1440 tcaggagtaa cttgtgctag aaagagtgat ttatacttga ctgctactcc atatcaactt 1500 tctcaagagt gatgcaatga tatcaacctt ttgaatttcg gtcaacccca ccaatagtg 1559 288 165 PRT NICOTIANATABACUM 288 Met Gln Phe Phe Asn Phe Ile Ser Phe Val Phe Phe Val Ser Phe Leu 1 5 10 15 Phe Leu Leu Arg Lys Trp Lys Asn Ser Asn Ser Gln Thr Lys Arg Leu 20 25 30 Pro Pro Gly Pro Trp Lys Leu Pro Val Leu Gly Ser Met Phe His Leu 35 40 45 Leu Gly Gly Pro Pro His His Val Leu Gly Asp Leu Ala Lys Lys Tyr 50 55 60 Gly Pro Leu Met His Leu Gln Leu Gly Glu Val Ser Val Val Ser Val 65 70 75 80 Thr Ser Pro Glu Met Ala Lys Glu Val Leu Lys Thr His Asp Leu Ala 85 90 95 Phe Ala Ser Arg Pro Leu Leu Leu Ala Ala Lys Ile Val Cys Tyr Asn 100 105 110 Gly Thr Asp Ile Val Phe Ser Pro Tyr Gly Asp Tyr Trp Arg Gln Thr 115 120 125 Arg Lys Ile Cys Leu Leu Glu Leu Leu Ser Ala Lys Asn Val Arg Ser 130 135 140 Phe Ser Ser Val Arg Arg Asp Glu Val Phe His Met Ile Glu Phe Phe 145 150 155 160 Ser Ile Ile Phe Trp 165 289 1592 DNA NICOTIANATABACUM 289 ccccaccaaa aaatcatttc tctcgtctaa aatggatctt ctcttactag agaagacctt 60 aattggtctt ttctttgcca ttttaatcgc tttaattgtc tctaaacttc gttcaaagcg 120 ttttaagctt cctccaggac caattccagt accagttttt ggtaattggc ttcaagttgg 180 tgatgattta aaccacagaa atcttactga ttatgccaag aaatttggag atcttttctt 240 gttaagaatg ggtcaacgta acttagttgt tgtgtcatct cctgaattag ctaaagaagt 300 tttacacaca caaggtgttg aatttggttc aagaacaaga aatgttgtgt ttgatatttt 360 tactggaaaa ggtcaagata tggtttttac tgtatatggt gaacattgga gaaaaatgag 420 gagaattatg actgtaccat tttttactaa taaagttgtg caacagtata gaggggggtg 480 ggagtttgag gtggcaagtg taattgagga tgtgaaaaaa aatcctgaat ctgctactaa 540 tgggatcgta ttaaggagga gattacaatt aatgatgtat aataatatgt ttaggattat 600 gtttgatagg agatttgaga gtgaagatga tcctttgttt gttaagctta aggctttgaa 660 tggtgaaagg agtagattgg ctcaaagttt tgagtataat tatggtgatt ttattccaat 720 tttgaggcct ttttttgaga ggttatttga agatctgtaa agaagttaag gagaagaggc 780 tgcagctttt caaagattac tttgttgatg aaagaaagaa gctttcgaat accaagagct 840 cggacagcaa tgccctaaaa tgtgcgattg atcacattct tgaggctcaa cagaagggag 900 agatcaatga ggacaacgtt ctttacattg ttgaaaacat caatgttgct gcaattgaaa 960 caacattatg gtcaattgag tggggtatcg ccgagctagt caaccaccct cacatccaaa 1020 agaaactgcg cgacgagatt gacacagttc ttggaccagg agtgcaagtg actgaaccag 1080 acacccacaa gcttccatac cttcaggctg tgatcaagga ggcacttcgt ctccgtatgg 1140 caattcctct attagtccca cacatgaacc ttcacgacgc aaagcttggc gggtttgata 1200 ttccagcaga gagcaaaatc ttggttaacg cttggtggtt agctaacaac ccggctcatt 1260 ggaagaaacc cgaagagttc agacccgaga ggttctttga agaggagaag catgttgagc 1320 caatggcaat gacttcagat atcttccgtt tggcgttggt aggaggagct gccctggaat 1380 tatacttgca ttgccaactc ttggcatcac tttgggacgt ttggttcaga atgttgcctc 1440 ctccaggcca gtcgaagctc gacaccacag agaaaggtgg acagttcagt ctccacattt 1500 tgaagcattc caccattgtg ttgaaaccaa ggtctttctg aactttgtga tcttattaat 1560 taaggggttc tgaagaaatt tgatagtgtt gg 1592 290 242 PRT NICOTIANATABACUM 290 Met Asp Leu Leu Leu Leu Glu Lys Thr Leu Ile Gly Leu Phe Phe Ala 1 5 10 15 Ile Leu Ile Ala Leu Ile Val Ser Lys Leu Arg Ser Lys Arg Phe Lys 20 25 30 Leu Pro Pro Gly Pro Ile Pro Val Pro Val Phe Gly Asn Trp Leu Gln 35 40 45 Val Gly Asp Asp Leu Asn His Arg Asn Leu Thr Asp Tyr Ala Lys Lys 50 55 60 Phe Gly Asp Leu Phe Leu Leu Arg Met Gly Gln Arg Asn Leu Val Val 65 70 75 80 Val Ser Ser Pro Glu Leu Ala Lys Glu Val Leu His Thr Gln Gly Val 85 90 95 Glu Phe Gly Ser Arg Thr Arg Asn Val Val Phe Asp Ile Phe Thr Gly 100 105 110 Lys Gly Gln Asp Met Val Phe Thr Val Tyr Gly Glu His Trp Arg Lys 115 120 125 Met Arg Arg Ile Met Thr Val Pro Phe Phe Thr Asn Lys Val Val Gln 130 135 140 Gln Tyr Arg Gly Gly Trp Glu Phe Glu Val Ala Ser Val Ile Glu Asp 145 150 155 160 Val Lys Lys Asn Pro Glu Ser Ala Thr Asn Gly Ile Val Leu Arg Arg 165 170 175 Arg Leu Gln Leu Met Met Tyr Asn Asn Met Phe Arg Ile Met Phe Asp 180 185 190 Arg Arg Phe Glu Ser Glu Asp Asp Pro Leu Phe Val Lys Leu Lys Ala 195 200 205 Leu Asn Gly Glu Arg Ser Arg Leu Ala Gln Ser Phe Glu Tyr Asn Tyr 210 215 220 Gly Asp Phe Ile Pro Ile Leu Arg Pro Phe Phe Glu Arg Leu Phe Glu 225 230 235 240 Asp Leu 291 1544 DNA NICOTIANATABACUM 291 ataatgctct ttctactctt tgtagccctt cctttcattc ttatttttct tcttcctaaa 60 ttcaaaaatg gtggaaataa cagattgcca ccaggtccta taggtttacc attcattgga 120 aatttgcatc aatatgatag tataactcct catatctatt tttggaaact ttccaaaaaa 180 tatggcaaaa tcttctcatt aaaacttgct tctactaatg tggtagtagt ttcttcagca 240 aaattagcaa aagaagtatt gaaaaaacaa gatttaatat tttgtagtag accatctatt 300 cttggccaac aaaaactgtc ttattatggt cgtgatattg cttttgcacc ttataatgat 360 tattggagag aaatgagaaa aatttgtgtt cttcatcttt ttagtttaaa aaaagttcaa 420 ttatttagtc caattcgtga agatgaagtt tttagaatga ttaagaaaat atcaaaacaa 480 gcttctactt cacaaattat taatttgagt aatttaatga tttcattaac aagtacaatt 540 atttgtagag ttgcttttgg tgttaggttt gaagaagaag cacatgcaag gaagagattt 600 gattttcttt tggccgaggc acaagaaatg atggctagtt tctttgtatc tgattttttt 660 ccctttttaa gttagattga caaattaagt ggattgacat atagacttga gaggaatttc 720 aaggatttgg ataattttta tgaagaactc attgagcaac atcaaaatcc taataagcca 780 aaatatatgg aaggagatat tgttgatctt ttgctacaat tgaagaaaga gaaattaaca 840 ccacttgatc tcactatgga agatataaaa ggaattctca tgaatgtgtt agttgcagga 900 tcagacacta gtgcagctgc tactgtttgg gcaatgacag ccttgataaa gaatcctaaa 960 gccatggaaa aagttcaatt agaaatcaga aaatcagttg ggaagaaagg cattgtaaat 1020 gaagaagatg tccaaaacat cccttatttt aaagcagtga taaaggaaat atttagattg 1080 tatccaccag ctccactttt agttccaaga gaatcaatgg aaaaaaccat attagaaggt 1140 tatgaaattc ggccaagaac catagttcat gttaacgctt gggctatagc aagggatcct 1200 gaaatatggg aaaatccaga tgaatttata cctgagagat ttttgaatag cagtatcgat 1260 tacaagggtc aagattttga gttacttcca tttggtgcag gcagaagagg ttgcccaggt 1320 attgcacttg gggttgcatc catggaactt gctttgtcaa atcttcttta tgcatttgat 1380 tgggagttgc cttatggagt gaaaaaagaa gacatcgaca caaacgttag gcctggaatt 1440 gccatgcaca agaaaaacga actttgcctt gtcccaaaaa aattatttat aaattatatt 1500 gggacgtgga tctcatgcta gttctgtgcg gtcagctaag ctta 1544 292 501 PRT NICOTIANATABACUM 292 Met Leu Phe Leu Leu Phe Val Ala Leu Pro Phe Ile Leu Ile Phe Leu 1 5 10 15 Leu Pro Lys Phe Lys Asn Gly Gly Asn Asn Arg Leu Pro Pro Gly Pro 20 25 30 Ile Gly Leu Pro Phe Ile Gly Asn Leu His Gln Tyr Asp Ser Ile Thr 35 40 45 Pro His Ile Tyr Phe Trp Lys Leu Ser Lys Lys Tyr Gly Lys Ile Phe 50 55 60 Ser Leu Lys Leu Ala Ser Thr Asn Val Val Val Val Ser Ser Ala Lys 65 70 75 80 Leu Ala Lys Glu Val Leu Lys Lys Gln Asp Leu Ile Phe Cys Ser Arg 85 90 95 Pro Ser Ile Leu Gly Gln Gln Lys Leu Ser Tyr Tyr Gly Arg Asp Ile 100 105 110 Ala Phe Ala Pro Tyr Trp Arg Glu Met Arg Lys Ile Cys Val Leu His 115 120 125 Leu Phe Ser Leu Lys Lys Val Gln Leu Phe Ser Pro Ile Arg Glu Asp 130 135 140 Glu Val Phe Arg Met Ile Lys Lys Ile Ser Lys Gln Ala Ser Thr Ser 145 150 155 160 Gln Ile Ile Asn Leu Ser Asn Leu Met Ile Ser Leu Thr Ser Thr Ile 165 170 175 Ile Cys Arg Val Ala Phe Gly Val Arg Phe Glu Glu Glu Ala His Ala 180 185 190 Arg Lys Arg Phe Asp Phe Leu Leu Ala Glu Ala Gln Glu Met Met Ala 195 200 205 Ser Phe Phe Val Ser Asp Phe Phe Pro Phe Leu Ser Ile Asp Lys Leu 210 215 220 Ser Gly Leu Thr Tyr Arg Leu Glu Arg Asn Phe Lys Asp Leu Asp Asn 225 230 235 240 Phe Tyr Glu Glu Leu Ile Glu Gln His Gln Asn Pro Asn Lys Pro Lys 245 250 255 Tyr Met Glu Gly Asp Ile Val Asp Leu Leu Leu Gln Leu Lys Lys Glu 260 265 270 Lys Leu Thr Pro Leu Asp Leu Thr Met Glu Asp Ile Lys Gly Ile Leu 275 280 285 Met Asn Val Leu Val Ala Gly Ser Asp Thr Ser Ala Ala Ala Thr Val 290 295 300 Trp Ala Met Thr Ala Leu Ile Lys Asn Pro Lys Ala Met Glu Lys Val 305 310 315 320 Gln Leu Glu Ile Arg Lys Ser Val Gly Lys Lys Gly Ile Val Asn Glu 325 330 335 Glu Asp Val Gln Asn Ile Pro Tyr Phe Lys Ala Val Ile Lys Glu Ile 340 345 350 Phe Arg Leu Tyr Pro Pro Ala Pro Leu Leu Val Pro Arg Glu Ser Met 355 360 365 Glu Lys Thr Ile Leu Glu Gly Tyr Glu Ile Arg Pro Arg Thr Ile Val 370 375 380 His Val Asn Ala Trp Ala Ile Ala Arg Asp Pro Glu Ile Trp Glu Asn 385 390 395 400 Pro Asp Glu Phe Ile Pro Glu Arg Phe Leu Asn Ser Ser Ile Asp Tyr 405 410 415 Lys Gly Gln Asp Phe Glu Leu Leu Pro Phe Gly Ala Gly Arg Arg Gly 420 425 430 Cys Pro Gly Ile Ala Leu Gly Val Ala Ser Met Glu Leu Ala Leu Ser 435 440 445 Asn Leu Leu Tyr Ala Phe Asp Trp Glu Leu Pro Tyr Gly Val Lys Lys 450 455 460 Glu Asp Ile Asp Thr Asn Val Arg Pro Gly Ile Ala Met His Lys Lys 465 470 475 480 Asn Glu Leu Cys Leu Val Pro Lys Lys Leu Phe Ile Asn Tyr Ile Gly 485 490 495 Thr Trp Ile Ser Cys 500 293 1764 DNA NICOTIANATABACUM 293 gtgaggtttg aatcctctgc ctcaatgaaa ctcaccaaat tggttttcta atttccatct 60 aaaatattgt ccaaagctaa agattctttc tccttaaata gtcaacttta gtggttcctc 120 ttcatttcat agctcaatct ttcttatttt gattcaacca tggagaacca atactcctac 180 tcattctctt cctacttcta cttagctata gtactgtttc ttcttccaat tttggtcaaa 240 tatttcttcc atcggagaag aaatttacct ccaagtccat tttctcttcc aataattggt 300 cacctttacc ttctcaagaa aactctccat ctcactctaa catccttatc agctaaatat 360 ggtcctgttt tatacctcaa attgggctct atgcctgtga ttgttgtgtc ctcaccatct 420 gctgttgaag aatgtttaac caagaatgat atcatattcg caaataggcc caagaccgtg 480 gctggtgaca agtttaccta caattatact gtttatgttt gggcacccta tggccaactt 540 tggagaattc ttcgccgatt aactgtcgtt gaactcttct cttcacatag cctacagaaa 600 acttctatcc ttagagatca agaagttgca atatttatcc gttcgttata caaattctca 660 aaggatagta gcaaaaaagt cgatttgacc aactggtctt ttactttggt tttcaatctt 720 atgaccaaaa ttattgctgg gagacatatt gtgaaggagg aagatgctgg caaggaaaag 780 ggcattgaaa ttattgaaaa acttagaggg actttcttag taactacatc attcttgaat 840 atgtgtgatt tcttgccagt attcaggtgg gttggttaca aagggctgga gaagaagatg 900 gcctcaattc acaatagaag aaatgaattc ttgaacagct tgcttgatga atttcgacac 960 aagaaaagta gtgcttcaca atctaacaca actgttggaa acatggagaa gaaaaccaca 1020 ctgattgaaa agctcttgtc tcttcaagaa tcagagcctg aattctacac tgatgatata 1080 tcaaaagtat tatgctggta gtttttgttg caggaacaga gacctcatca acaaccatcc 1140 aatgggtaat gaggcttctt gtagctcacc ctgaggcatt gtataagcta cgagctgaca 1200 ttgacagtaa agttgggaat aagcgcttgc tgaatgaatc agacctcaac aagcttccgt 1260 atttgcattg tgttgttaat gagacaatga gattatacac tccgatacca cttttattgc 1320 ctcattattc aactaaagat tgtattgtgg aaggatatga tgtaccaaaa catacaatgt 1380 tgtttgtcaa cgcttgggcc attcacaggg atcccaaggt atgggaggag cctgacaagt 1440 tcaagccaga gagatttgag gcaacagaag gggaaacaga aaggttcaat tacaagcttg 1500 taccatttgg aatggggaga agagcgtgcc ctggagctga tatggggttg cgagcagttt 1560 ctttggcatt aggtgcactt attcaatgct ttgactggca aattgaggaa gcggaaagct 1620 tggaggaaag ctataattct agaatgacta tgcagaacaa gcctttgaag gttgtctgca 1680 ctccacgcga agatcttggc cagcttctat cccaactcta aggcaattta tcaatgccaa 1740 acgtaatctt catctaccac tatg 1764 294 520 PRT NICOTIANATABACUM 294 Met Glu Asn Gln Tyr Ser Tyr Ser Phe Ser Ser Tyr Phe Tyr Leu Ala 1 5 10 15 Ile Val Leu Phe Leu Leu Pro Ile Leu Val Lys Tyr Phe Phe His Arg 20 25 30 Arg Arg Asn Leu Pro Pro Ser Pro Phe Ser Leu Pro Ile Ile Gly His 35 40 45 Leu Tyr Leu Leu Lys Lys Thr Leu His Leu Thr Leu Thr Ser Leu Ser 50 55 60 Ala Lys Tyr Gly Pro Val Leu Tyr Leu Lys Leu Gly Ser Met Pro Val 65 70 75 80 Ile Val Val Ser Ser Pro Ser Ala Val Glu Glu Cys Leu Thr Lys Asn 85 90 95 Asp Ile Ile Phe Ala Asn Arg Pro Lys Thr Val Ala Gly Asp Lys Phe 100 105 110 Thr Tyr Asn Tyr Thr Val Tyr Val Trp Ala Pro Tyr Gly Gln Leu Trp 115 120 125 Arg Ile Leu Arg Arg Leu Thr Val Val Glu Leu Phe Ser Ser His Ser 130 135 140 Leu Gln Lys Thr Ser Ile Leu Arg Asp Gln Glu Val Ala Ile Phe Ile 145 150 155 160 Arg Ser Leu Tyr Lys Phe Ser Lys Asp Ser Ser Lys Lys Val Asp Leu 165 170 175 Thr Asn Trp Ser Phe Thr Leu Val Phe Asn Leu Met Thr Lys Ile Ile 180 185 190 Ala Gly Arg His Ile Val Lys Glu Glu Asp Ala Gly Lys Glu Lys Gly 195 200 205 Ile Glu Ile Ile Glu Lys Leu Arg Gly Thr Phe Leu Val Thr Thr Ser 210 215 220 Phe Leu Asn Met Cys Asp Phe Leu Pro Val Phe Arg Trp Val Gly Tyr 225 230 235 240 Lys Gly Leu Glu Lys Lys Met Ala Ser Ile His Asn Arg Arg Asn Glu 245 250 255 Phe Leu Asn Ser Leu Leu Asp Glu Phe Arg His Lys Lys Ser Ser Ala 260 265 270 Ser Gln Ser Asn Thr Thr Val Gly Asn Met Glu Lys Lys Thr Thr Leu 275 280 285 Ile Glu Lys Leu Leu Ser Leu Gln Glu Ser Glu Pro Glu Phe Tyr Thr 290 295 300 Asp Asp Ile Ile Lys Ser Ile Met Leu Val Val Phe Val Ala Gly Thr 305 310 315 320 Glu Thr Ser Ser Thr Thr Ile Gln Trp Val Met Arg Leu Leu Val Ala 325 330 335 His Pro Glu Ala Leu Tyr Lys Leu Arg Ala Asp Ile Asp Ser Lys Val 340 345 350 Gly Asn Lys Arg Leu Leu Asn Glu Ser Asp Leu Asn Lys Leu Pro Tyr 355 360 365 Leu His Cys Val Val Asn Glu Thr Met Arg Leu Tyr Thr Pro Ile Pro 370 375 380 Leu Leu Leu Pro His Tyr Ser Thr Lys Asp Cys Ile Val Glu Gly Tyr 385 390 395 400 Asp Val Pro Lys His Thr Met Leu Phe Val Asn Ala Trp Ala Ile His 405 410 415 Arg Asp Pro Lys Val Trp Glu Glu Pro Asp Lys Phe Lys Pro Glu Arg 420 425 430 Phe Glu Ala Thr Glu Gly Glu Thr Glu Arg Phe Asn Tyr Lys Leu Val 435 440 445 Pro Phe Gly Met Gly Arg Arg Ala Cys Pro Gly Ala Asp Met Gly Leu 450 455 460 Arg Ala Val Ser Leu Ala Leu Gly Ala Leu Ile Gln Cys Phe Asp Trp 465 470 475 480 Gln Ile Glu Glu Ala Glu Ser Leu Glu Glu Ser Tyr Asn Ser Arg Met 485 490 495 Thr Met Gln Asn Lys Pro Leu Lys Val Val Cys Thr Pro Arg Glu Asp 500 505 510 Leu Gly Gln Leu Leu Ser Gln Leu 515 520 295 1694 DNA NICOTIANATABACUM 295 aacatccttt ccttcttcca aaaatggagc ttcaatcttc tcctttcaat ttaatttctt 60 tgttcctctt cttttctttt ctttttattc tagtgaagaa atggaatgcc aaaatcccaa 120 agttacctcc aggtccgtgg aggcttccct ttattggaag cctccatcac ttgaagggaa 180 aacttccaca ccataatctt agagatctag cgcgaaaata tggacctctc atgtacttac 240 aactcggaga aattcctgta gttgtaatat cttcgccacg tgtagcaaaa gctgtactaa 300 aaactcatga tctcgctttt gcaactagac cacgattcat gtcctcagac attgtgtttt 360 acaaaagcag ggacatctct tttgccccat ttggtgatta ctggagacag atgcgtaaaa 420 tattgactca ggaactcctg agcaacaaga tgctcaagtc atatagctta atccgaaagg 480 atgagctctc gaagctcctc tcatcgattc gtttggaaac aggttctgca gtgaacataa 540 atgaaaagct tctctggttt acgagctgca tgacctgtag attagccttt ggaaaaatat 600 gcaatgatcg ggatgagttg atcatgctaa ttagggagat attaacatta tcaggaggat 660 ttgatgtggg tgatttgttc ccttcctgga aattacttca taatatgagc aacatgaaag 720 ctaggttgac gaatgtacac cacaagtatg atttagttat ggagaacatc atcaatgagc 780 accaagagaa tcatgcagca gggataaagg gtaacaacga gtttggtggc gaagatatga 840 tcgatgctct actgagggct aaggagaata atgagcttca atttcctatc gaaaatgaca 900 acatgaaagc agtaattctg gacttgttta ttgctggaac tgaaacttca tatactgcaa 960 ttatatgggc actatcagaa ttgatgaagc acccaagtgt gatggccaag gcacaagctg 1020 aagtgagaaa agtcttcaaa gaaaatgaaa atttcgacga aaatgatctt gacaagttgc 1080 catacctaaa atcagtgatt aaagaaacac taaggatgca ccctccagtt cctttgttag 1140 ggcctagaga atgcagggac caaacagaga tcgatggcta cactgtacct attaaagcta 1200 gagttatggt taatgcttgg gcgataggaa gagatcctga aagttgggaa gatcctgaaa 1260 gtttcaaacc ggagcgattt gaaaatactt ctgttgatct tacaggaaat cactatcagt 1320 tcattccttt cggttcagga agaagaatgt gtccaggaat gtcgtttggt ttagttaaca 1380 cagggcatcc tttagcccag ttgctctatt gctttgactg gaaactccct gacaaggtta 1440 atgcaaatga ttttcgcact actgaaacaa gtagagtttt tgcagcaagc aaagatgacc 1500 tctacttgat tcccacaaat cacagggagc aagaatagct taatttaatg gagttcttgg 1560 aagaattaaa gaagaagggc tatataggtg agattttttg tatggttgca aggtttttag 1620 ttcatacaat aagacaatac attatattcc agtattgtgt atcatgtata ataaggttcc 1680 ttttgtttaa aaaa 1694 296 504 PRT NICOTIANATABACUM 296 Met Glu Leu Gln Ser Ser Pro Phe Asn Leu Ile Ser Leu Phe Leu Phe 1 5 10 15 Phe Ser Phe Leu Phe Ile Leu Val Lys Lys Trp Asn Ala Lys Ile Pro 20 25 30 Lys Leu Pro Pro Gly Pro Trp Arg Leu Pro Phe Ile Gly Ser Leu His 35 40 45 His Leu Lys Gly Lys Leu Pro His His Asn Leu Arg Asp Leu Ala Arg 50 55 60 Lys Tyr Gly Pro Leu Met Tyr Leu Gln Leu Gly Glu Ile Pro Val Val 65 70 75 80 Val Ile Ser Ser Pro Arg Val Ala Lys Ala Val Leu Lys Thr His Asp 85 90 95 Leu Ala Phe Ala Thr Arg Pro Arg Phe Met Ser Ser Asp Ile Val Phe 100 105 110 Tyr Lys Ser Arg Asp Ile Ser Phe Ala Pro Phe Gly Asp Tyr Trp Arg 115 120 125 Gln Met Arg Lys Ile Leu Thr Gln Glu Leu Leu Ser Asn Lys Met Leu 130 135 140 Lys Ser Tyr Ser Leu Ile Arg Lys Asp Glu Leu Ser Lys Leu Leu Ser 145 150 155 160 Ser Ile Arg Leu Glu Thr Gly Ser Ala Val Asn Ile Asn Glu Lys Leu 165 170 175 Leu Trp Phe Thr Ser Cys Met Thr Cys Arg Leu Ala Phe Gly Lys Ile 180 185 190 Cys Asn Asp Arg Asp Glu Leu Ile Met Leu Ile Arg Glu Ile Leu Thr 195 200 205 Leu Ser Gly Gly Phe Asp Val Gly Asp Leu Phe Pro Ser Trp Lys Leu 210 215 220 Leu His Asn Met Ser Asn Met Lys Ala Arg Leu Thr Asn Val His His 225 230 235 240 Lys Tyr Asp Leu Val Met Glu Asn Ile Ile Asn Glu His Gln Glu Asn 245 250 255 His Ala Ala Gly Ile Lys Gly Asn Asn Glu Phe Gly Gly Glu Asp Met 260 265 270 Ile Asp Ala Leu Leu Arg Ala Lys Glu Asn Asn Glu Leu Gln Phe Pro 275 280 285 Ile Glu Asn Asp Asn Met Lys Ala Val Ile Leu Asp Leu Phe Ile Ala 290 295 300 Gly Thr Glu Thr Ser Tyr Thr Ala Ile Ile Trp Ala Leu Ser Glu Leu 305 310 315 320 Met Lys His Pro Ser Val Met Ala Lys Ala Gln Ala Glu Val Arg Lys 325 330 335 Val Phe Lys Glu Asn Glu Asn Phe Asp Glu Asn Asp Leu Asp Lys Leu 340 345 350 Pro Tyr Leu Lys Ser Val Ile Lys Glu Thr Leu Arg Met His Pro Pro 355 360 365 Val Pro Leu Leu Gly Pro Arg Glu Cys Arg Asp Gln Thr Glu Ile Asp 370 375 380 Gly Tyr Thr Val Pro Ile Lys Ala Arg Val Met Val Asn Ala Trp Ala 385 390 395 400 Ile Gly Arg Asp Pro Glu Ser Trp Glu Asp Pro Glu Ser Phe Lys Pro 405 410 415 Glu Arg Phe Glu Asn Thr Ser Val Asp Leu Thr Gly Asn His Tyr Gln 420 425 430 Phe Ile Pro Phe Gly Ser Gly Arg Arg Met Cys Pro Gly Met Ser Phe 435 440 445 Gly Leu Val Asn Thr Gly His Pro Leu Ala Gln Leu Leu Tyr Cys Phe 450 455 460 Asp Trp Lys Leu Pro Asp Lys Val Asn Ala Asn Asp Phe Arg Thr Thr 465 470 475 480 Glu Thr Ser Arg Val Phe Ala Ala Ser Lys Asp Asp Leu Tyr Leu Ile 485 490 495 Pro Thr Asn His Arg Glu Gln Glu 500 297 1819 DNA NICOTIANATABACUM 297 caatcagtgg atgcgggagt aatatataat atgcaagttg tagaaagaga aaaaaaaaat 60 caagtagcta ttctatactg gggcacaaat agtgagtgaa aatggagact gttcaaatca 120 taataacagc atcttgtgct gccataataa ttactctagt ggtgtgtatt tggagagtac 180 tgaattgggt ttggttcaga ccaaagaagc tggaaaaact attgaggaaa caaggtctca 240 aaggcaactc ctacaagatt ttgtatgggg atatgaagga gctttctggt atgattaagg 300 aagctaattc caaacccatg aatctttctg atgatattgc accaagattg gtgcctttct 360 ttcttgacac catcaagaaa tatggtaaaa aatcctttgt atggttaggt ccgaaaccac 420 tggttcttat catggaccct gagcttataa aggaaatatt ttccaaatac tatctgtatc 480 aaaagcctca tggaaatcca gttaccaagc tattagtaca aggactagta agcctagagg 540 aagacaaatg ggccaaacat agaaaaatca tcaatccagc tttccatcta gagaagctaa 600 agcatatgct tccagctttt tgcttgagct gcactgagat gctgtgcaaa tgggaagata 660 ttgtttcaat taagggctca catgagatag atgtatggcc tcaccttgaa caattaagta 720 gcgatgtgat ctctcggaca gcttttggca gtaactttga agaaggtaaa aggatatttg 780 aacttcagaa ggaacaagct cagtattttg tagaagctat acgctcggtt tatataccag 840 gctggaggtt tttgccaaca aagaggaaca gaagaatgaa ggaagttgaa aaggatgttc 900 gggcctcgat aagaggcatt attgataaaa gagtgaaggc aatgaaagca ggagaggcga 960 gtaatgagga tctacttggt atattgttgg aatctaattt tacagaagct gaacagcata 1020 gacacaagga ttctgcgatg agcattgaag aagtcattca agaatgcaag ttattctatg 1080 ttgctggcca agaaactaca tcagtgttgc ttgtgtggac tctaatattg ttgagtaggc 1140 atcaagattg gcagagccga gccagagaag aggtgtttca agtctttggt aatcagaaac 1200 cagattttga cggattgaat cgtctaaaag ttgtgacaat gatcttgtat gagtctttaa 1260 ggctatactc cccagtagtg tcactaatcc ggcggcctaa tgaggatgct atattaggaa 1320 atgtatctct gccagaaggt gtgctactct cattaccagt gatcttatta caccacgatg 1380 aagagatatg gggtaaagat gcaaagaagt tcaatccaga aagatttaga gatggagtct 1440 caagtgcaac aaagggtcaa gtcacttttt ttccatttac ttggggtccc agaatatgca 1500 tcggacaaaa ttttgccatg ttagaagcaa agactgcttt ggctatgatc ctacaacgct 1560 tctcattcga actgtctcca tcttatgcac atgctcctca gtccatatta actatgcaac 1620 cccaacatgg tgctccacta attctgcaca aaatatagtt tgttacttta agcagtgtct 1680 tgttatatgt cagagagtcc aaaatgttta attaaggctt gtagaactgc caaatggaac 1740 ttcatttgca ttcgtgggtt gtagattgtt gtaattggac aagtatactg tttattttag 1800 agttttaaga aaaaaaaaa 1819 298 518 PRT NICOTIANATABACUM 298 Met Glu Thr Val Gln Ile Ile Ile Thr Ala Ser Cys Ala Ala Ile Ile 1 5 10 15 Ile Thr Leu Val Val Cys Ile Trp Arg Val Leu Asn Trp Val Trp Phe 20 25 30 Arg Pro Lys Lys Leu Glu Lys Leu Leu Arg Lys Gln Gly Leu Lys Gly 35 40 45 Asn Ser Tyr Lys Ile Leu Tyr Gly Asp Met Lys Glu Leu Ser Gly Met 50 55 60 Ile Lys Glu Ala Asn Ser Lys Pro Met Asn Leu Ser Asp Asp Ile Ala 65 70 75 80 Pro Arg Leu Val Pro Phe Phe Leu Asp Thr Ile Lys Lys Tyr Gly Lys 85 90 95 Lys Ser Phe Val Trp Leu Gly Pro Lys Pro Leu Val Leu Ile Met Asp 100 105 110 Pro Glu Leu Ile Lys Glu Ile Phe Ser Lys Tyr Tyr Leu Tyr Gln Lys 115 120 125 Pro His Gly Asn Pro Val Thr Lys Leu Leu Val Gln Gly Leu Val Ser 130 135 140 Leu Glu Glu Asp Lys Trp Ala Lys His Arg Lys Ile Ile Asn Pro Ala 145 150 155 160 Phe His Leu Glu Lys Leu Lys His Met Leu Pro Ala Phe Cys Leu Ser 165 170 175 Cys Thr Glu Met Leu Cys Lys Trp Glu Asp Ile Val Ser Ile Lys Gly 180 185 190 Ser His Glu Ile Asp Val Trp Pro His Leu Glu Gln Leu Ser Ser Asp 195 200 205 Val Ile Ser Arg Thr Ala Phe Gly Ser Asn Phe Glu Glu Gly Lys Arg 210 215 220 Ile Phe Glu Leu Gln Lys Glu Gln Ala Gln Tyr Phe Val Glu Ala Ile 225 230 235 240 Arg Ser Val Tyr Ile Pro Gly Trp Arg Phe Leu Pro Thr Lys Arg Asn 245 250 255 Arg Arg Met Lys Glu Val Glu Lys Asp Val Arg Ala Ser Ile Arg Gly 260 265 270 Ile Ile Asp Lys Arg Val Lys Ala Met Lys Ala Gly Glu Ala Ser Asn 275 280 285 Glu Asp Leu Leu Gly Ile Leu Leu Glu Ser Asn Phe Thr Glu Ala Glu 290 295 300 Gln His Arg His Lys Asp Ser Ala Met Ser Ile Glu Glu Val Ile Gln 305 310 315 320 Glu Cys Lys Leu Phe Tyr Val Ala Gly Gln Glu Thr Thr Ser Val Leu 325 330 335 Leu Val Trp Thr Leu Ile Leu Leu Ser Arg His Gln Asp Trp Gln Ser 340 345 350 Arg Ala Arg Glu Glu Val Phe Gln Val Phe Gly Asn Gln Lys Pro Asp 355 360 365 Phe Asp Gly Leu Asn Arg Leu Lys Val Val Thr Met Ile Leu Tyr Glu 370 375 380 Ser Leu Arg Leu Tyr Ser Pro Val Val Ser Leu Ile Arg Arg Pro Asn 385 390 395 400 Glu Asp Ala Ile Leu Gly Asn Val Ser Leu Pro Glu Gly Val Leu Leu 405 410 415 Ser Leu Pro Val Ile Leu Leu His His Asp Glu Glu Ile Trp Gly Lys 420 425 430 Asp Ala Lys Lys Phe Asn Pro Glu Arg Phe Arg Asp Gly Val Ser Ser 435 440 445 Ala Thr Lys Gly Gln Val Thr Phe Phe Pro Phe Thr Trp Gly Pro Arg 450 455 460 Ile Cys Ile Gly Gln Asn Phe Ala Met Leu Glu Ala Lys Thr Ala Leu 465 470 475 480 Ala Met Ile Leu Gln Arg Phe Ser Phe Glu Leu Ser Pro Ser Tyr Ala 485 490 495 His Ala Pro Gln Ser Ile Leu Thr Met Gln Pro Gln His Gly Ala Pro 500 505 510 Leu Ile Leu His Lys Ile 515

Claims (18)

What is claimed is:
1. An isolated nucleic acid molecule from Nicotiana, wherein said nucleic acid molecule comprises a nucleic acid sequence selected from the group consisting of SEQ. ID. No.:149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239, 241, 243, 245, 247, 249, 251, 253, 255, 257, 259, 261, 263, 265, 267, 269, 271, 273, 275, 277, 279, 281, 283, 285, 287, 289, 291, 293, 295 and 297.
2. An isolated nucleic acid molecule from Nicotiana wherein said nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ. ID. No. 299 through SEQ. ID. No. 357.
3. An isolated protein from Nicotiana, wherein said protein comprises an amino acid sequence selected from the group consisting of SEQ. ID. No.:. 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292, 294, 296 and 298.
4. A transgenic plant, wherein said transgenic plant comprises the nucleic acid molecule of claim 1 or 2.
5. The transgenic plant of claim 4, wherein said plant is a tobacco plant.
6. A method of producing a transgenic plant, wherein said method comprises the steps of:
(i) operably linking said nucleic acid molecule of any one of the claims 1 or 2 with a promoter functional in said plant to create a plant transformational vector;
(ii) transforming said plant with said plant transformational vector of step;
(iii) selecting a plant cell transformed with said transformation vector; and
(iv) regenerating a transformation plant from said transformed plant cell.
7. The method of claim 6, wherein said nucleic acid molecule is in an antisense orientation.
8. The method of claim 6, wherein said nucleic acid molecule is in a sense orientation.
9. The method of claim 6, wherein said nucleic acid molecule is in a RNA interference orientation.
10. The method of claim 6, wherein said nucleic acid molecule is expressed as a double stranded RNA molecule.
11. The method of claim 6, wherein said double stranded RNA molecule is about 15 to 25 nucleotides in length.
12. The method of claim 6, wherein said transgenic plant is a tobacco plant.
13. A method of selecting a plant containing a nucleic acid molecule, wherein said plant is analyzed for the presence of nucleic acid sequence selected from the group consisting of 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239, 241, 243, 245, 247, 249, 251, 253, 255, 257, 259, 261, 263, 265, 267, 269, 271, 273, 275, 277, 279, 281, 283, 285, 287, 289, 291, 293, 295 and 297.
14. The method of selecting a plant of claim 13, wherein said plant is analyzed by DNA hybridization.
15. The method of selecting a plant of claim 13, wherein said DNA hybridization is Southern blot analysis.
16. The method of selecting a plant of claim 13, wherein said DNA hybridization is Northern blot analysis.
17. The method of selecting a plant of claim 13, wherein said plant is analyzed by PCR detection.
18. The method of claim 13, wherein said plant is a tobacco plant.
US10/686,947 2001-11-13 2003-10-16 Cloning of cytochrome p450 genes from nicotiana Abandoned US20040162420A1 (en)

Priority Applications (44)

Application Number Priority Date Filing Date Title
US10/686,947 US20040162420A1 (en) 2002-10-16 2003-10-16 Cloning of cytochrome p450 genes from nicotiana
US10/934,944 US7812227B2 (en) 2001-11-13 2004-09-03 Cloning of cytochrome p450 genes from nicotiana
US10/943,507 US7855318B2 (en) 2001-11-13 2004-09-17 Cloning of cytochrome P450 genes from Nicotiana
PCT/US2004/034065 WO2005038033A2 (en) 2003-10-16 2004-10-15 Cloning of cytochrome p450 genes from nicotiana
JP2006535343A JP5422099B2 (en) 2003-10-16 2004-10-15 Cloning of cytochrome p450 gene from Nicotiana
JP2006535378A JP4519137B2 (en) 2003-10-16 2004-10-15 Cloning of cytochrome p450 gene from Nicotiana
BRPI0415362-6A BRPI0415362A (en) 2003-10-16 2004-10-15 nicotine-isolated nucleic acid molecule, nicotine-isolated protein, transgenic plant, methods of producing a transgenic plant, selecting a plant containing a nucleic acid molecule, and increasing or decreasing nornicotine levels in a plant , tobacco product and leaf having reduced amounts of nornicotin levels, and method of isolating a gene from a plant
PL04795252T PL1675957T3 (en) 2003-10-16 2004-10-15 Cloning of cytochrome p450 genes from nicotiana
PL04795391T PL1694832T3 (en) 2003-10-16 2004-10-15 Use of a cytochrome p450 gene from nicotiana
AU2004282588A AU2004282588B2 (en) 2002-10-16 2004-10-15 Cloning of cytochrome P450 genes from nicotiana
BRPI0415363-4A BRPI0415363A (en) 2003-10-16 2004-10-15 nucleic acid molecule isolated from nicotine, transgenic plant, and methods of producing a transgenic plant, selecting a plant containing a nucleic acid molecule, and increasing or decreasing nornicotine levels in a plant
CN200480030387XA CN1926235B (en) 2003-10-16 2004-10-15 Cloning of cytochrome P450 genes from nicotiana
AU2004282576A AU2004282576B2 (en) 2002-10-16 2004-10-15 Cloning of cytochrome P450 genes from nicotiana
ZA200602938A ZA200602938B (en) 2003-10-16 2004-10-15 Cloning of cytochrome p450 genes from Nicotiana
EP10012983A EP2330191A1 (en) 2003-10-16 2004-10-15 Cloning of cytochrome P450 genes from nicotiana
ES04795252T ES2377868T3 (en) 2003-10-16 2004-10-15 Cloning of genes encoding cytochrome p450 from Nicotiana
CN2004800303850A CN1867674B (en) 2003-10-16 2004-10-15 Cloning of cytochrome P450 genes from nicotiana
CN2012101717953A CN102703477A (en) 2003-10-16 2004-10-15 Cloning of cytochrome p450 genes from nicotiana
EP04795252A EP1675957B1 (en) 2003-10-16 2004-10-15 Cloning of cytochrome p450 genes from nicotiana
CN2011102770337A CN102321641A (en) 2003-10-16 2004-10-15 From the tobacco cloning of cytochrome P 450 genes
PCT/US2004/034218 WO2005038018A2 (en) 2003-10-16 2004-10-15 Cloning of cytochrome p450 genes from nicotiana
AT04795252T ATE536415T1 (en) 2003-10-16 2004-10-15 CLONING OF THE CYTOCHROME P450 GENE OF NICOTIANA
EP04795391A EP1694832B1 (en) 2003-10-16 2004-10-15 Use of a cytochrome p450 gene from nicotiana
AT04795391T ATE554161T1 (en) 2003-10-16 2004-10-15 USE OF A CYTOCHROME P450 GENE FROM NICOTIANA
ES04795391T ES2384243T3 (en) 2003-10-16 2004-10-15 Use of a gene for nicotiana cytochrome P450
US11/110,062 US7700851B2 (en) 2001-11-13 2005-04-19 Tobacco nicotine demethylase genomic clone and uses thereof
US11/116,881 US7700834B2 (en) 2001-11-13 2005-04-27 Nicotiana nucleic acid molecules and uses thereof
US11/694,507 US20070292871A1 (en) 2002-10-16 2007-03-30 Method of screening for cytochrome p450s
HK07109796.0A HK1104575A1 (en) 2003-10-16 2007-09-07 Cloning of cytochrome p450 genes from nicotiana
JP2009276020A JP5558797B2 (en) 2003-10-16 2009-12-04 Cloning of cytochrome p450 gene from Nicotiana
US12/755,733 US8581043B2 (en) 2001-11-13 2010-04-07 Nicotiana nucleic acid molecules and uses thereof
US12/760,905 US8592663B2 (en) 2001-11-13 2010-04-15 Tobacco nicotine demethylase genomic clone and uses thereof
US12/762,613 US8637731B2 (en) 2003-10-16 2010-04-19 Nicotiana nucleic acid molecules and uses thereof
AU2010201696A AU2010201696B2 (en) 2002-10-16 2010-04-28 Cloning of cytochrome P450 genes from nicotiana
AU2010201695A AU2010201695B2 (en) 2002-10-16 2010-04-28 Cloning of cytochrome P450 genes from nicotiana
US12/821,273 US8058504B2 (en) 2001-11-13 2010-06-23 Cloning of cytochrome P450 genes from Nicotiana
JP2010166914A JP5721357B2 (en) 2003-10-16 2010-07-26 Cloning of cytochrome p450 gene from Nicotiana
US12/901,878 US8436235B2 (en) 2001-11-13 2010-10-11 Cloning of cytochrome p450 genes from Nicotiana
US13/243,270 US8188337B2 (en) 2001-11-13 2011-09-23 Cloning of cytochrome p450 genes from Nicotiana
US13/481,297 US8658856B2 (en) 2001-11-13 2012-05-25 Cloning of cytochrome p450 genes from Nicotiana
US13/887,913 US9464297B2 (en) 2001-11-13 2013-05-06 Cloning of cytochrome P450 genes from nicotiana
US14/087,204 US9322030B2 (en) 2001-11-13 2013-11-22 Tobacco nicotine demethylase genomic clone and uses thereof
US15/136,624 US10266836B2 (en) 2001-11-13 2016-04-22 Tobacco nicotine demethylase genomic clone and uses thereof
US16/289,210 US10954526B2 (en) 2001-11-13 2019-02-28 Tobacco nicotine demethylase genomic clone and uses thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US41893302P 2002-10-16 2002-10-16
US10/397,346 US6761492B2 (en) 2002-03-28 2003-03-27 Image forming device
US48536803P 2003-07-08 2003-07-08
US10/686,947 US20040162420A1 (en) 2002-10-16 2003-10-16 Cloning of cytochrome p450 genes from nicotiana

Related Parent Applications (6)

Application Number Title Priority Date Filing Date
US10/293,252 Continuation-In-Part US20040103449A1 (en) 2001-11-13 2002-11-13 Identification and use of cytochrome P450 nucleic acid sequences from tobacco
US10/387,346 Continuation-In-Part US20040117869A1 (en) 2001-11-13 2003-03-12 Cloning of cytochrome P450 genes from Nicotiana
US10/397,346 Continuation-In-Part US6761492B2 (en) 2002-03-28 2003-03-27 Image forming device
US10/686,947 Continuation-In-Part US20040162420A1 (en) 2001-11-13 2003-10-16 Cloning of cytochrome p450 genes from nicotiana
PCT/US2004/034218 Continuation-In-Part WO2005038018A2 (en) 2001-11-13 2004-10-15 Cloning of cytochrome p450 genes from nicotiana
US11/110,062 Continuation-In-Part US7700851B2 (en) 2001-11-13 2005-04-19 Tobacco nicotine demethylase genomic clone and uses thereof

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US10/934,944 Continuation-In-Part US7812227B2 (en) 2001-11-13 2004-09-03 Cloning of cytochrome p450 genes from nicotiana
US10/934,944 Continuation US7812227B2 (en) 2001-11-13 2004-09-03 Cloning of cytochrome p450 genes from nicotiana
US10/943,507 Continuation-In-Part US7855318B2 (en) 2001-11-13 2004-09-17 Cloning of cytochrome P450 genes from Nicotiana
US11/694,507 Continuation US20070292871A1 (en) 2002-10-16 2007-03-30 Method of screening for cytochrome p450s
US13/887,913 Continuation-In-Part US9464297B2 (en) 2001-11-13 2013-05-06 Cloning of cytochrome P450 genes from nicotiana

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