TWI637171B - Test sheet for occult blood - Google Patents

Abstract

The invention is a occult blood test paper comprising a liquid permeable carrier layer, a detection layer and an outer layer, wherein the liquid permeable carrier layer and the detection layer are connected in parallel or stacked on the outer layer from top to bottom. The liquid permeable carrier layer can carry a biological sample, the detection layer contains the detection substance, and the outer layer prevents the user from coming into contact with the biological sample. The upper surface of the junction of the liquid permeable carrier layer and the detection layer is formed with at least one folding line, or the upper surface of the liquid permeable carrier layer has a folding line for facilitating bending. When the occult blood test paper is bent along the folding line, the biological sample can be in contact with the test substance by infiltration and react, and whether the biological sample contains occult blood by the discoloration of the reaction. Therefore, the present invention has a simple structure, is convenient to operate, and is quite hygienic, and is suitable for the general public to perform occult blood testing alone.

Description

Occult blood test strip

The invention relates to a occult blood test paper, in particular, the occult blood test paper is bent along at least one folding line and folded in half, so that the biological sample contacts the detecting substance by the infiltration and reacts, and whether the biological sample contains occult blood by the discoloration of the reaction. And to determine whether there is the possibility of colorectal cancer, it is convenient for people to conduct colorectal cancer screening in the home space, greatly improving the penetration rate of screening.

Among the top ten cancers, the incidence of colorectal cancer has climbed to the first and second places in recent years. The incidence of colorectal cancer in Taiwan has increased steadily due to factors such as dietary westernization and frequent food safety problems. Because of the high incidence of colorectal cancer, how to screen for early diagnosis of disease has become a worldwide common issue. Fortunately, the disease progression of colorectal cancer is generally slow. It usually takes about ten years from the progression of low-grade intestinal polyps to invasive cancers with high malignancy. Therefore, if it can be diagnosed early in the early stage of colorectal cancer and treated early, the mortality of colorectal cancer can be greatly reduced. Early detection and early treatment can not only save a lot of people's lives, but also avoid the pain of treating serious cancer, and thus save considerable social costs.

Due to the possible benefits of colorectal cancer screening, a variety of screening methods have been developed. Mainly include: chemical methods, immunology, colonoscopy. Among them, the principle of chemical method is to use hemoglobin in fecal occult blood: when there is occult blood in the feces, the iron ions in hemoglobin will react with the added reagent to produce redox reaction, and the result is determined by the coloring agent. in accordance with. The immunological and chemical methods are the same as the fecal examination, which uses latex micro-antibody with human heme-targeted antibodies. Granules to detect hemoglobin in the feces. When there is occult blood in the feces, the special antibody on the latex particles will bind to heme. In the laboratory, the turbidity of the fecal occult blood is quantified by the turbidity method. In terms of medical economics, immunization involves the use of specific antibodies, which are not only expensive, but the preservation and use of reagents is also a challenge for many laboratories. In contrast, unlike chemical and immunological methods, colonoscopy screening is performed directly by endoscopic methods for examination of the wall of the large intestine. The advantage is that when a lesion is found, the physician can directly remove the lesion by colonoscopy. With the benefits of diagnosis and treatment. However, due to its invasive nature, it is estimated that every thousand colonoscopes operated may cause serious complications of one to three large intestine ruptures. In summary, chemical screening has several advantages: excellent sensitivity and specificity, low price, non-invasive, and no complications. Therefore, compared with the immunological method and colonoscopy, the chemical method is more suitable as a method for colorectal cancer screening.

However, in terms of procedures, the current screening methods have their inconveniences, which may affect the popularity of screening. Both chemical and immunological methods are fecal tests. The current main procedures include: fecal specimens, fecal specimens, laboratory tests, and nuclear reports. On the whole, the process is quite complicated, and the patient must take samples of the stool sample from the feces. In addition, the process of sending inspections, inspections, and reports is lengthy, and such inconvenient and cumbersome processes may reduce the willingness of the public to accept screening. When accepting colonoscopy, people need to go to the hospital for examination. Factors such as low accessibility, inadequate testing equipment, relatively scarce gastroenterologists, and anesthesiologists may significantly reduce the prevalence of colorectal cancer screening.

Therefore, there is a need for a novel occult blood test strip, which uses a occult blood test strip to be folded in half along at least one fold line, so that the biological sample contacts the test substance by infiltration and reacts, and whether the biological sample contains occult blood by using the reaction color change, so the use Just wipe and fold The occult blood can be identified without any pre-treatment or post-treatment of the biological sample, and in particular, does not cause any discomfort to the user, and is quite hygienic, thereby solving the problems of the above-mentioned prior art.

The main object of the present invention is to provide a occult blood test paper comprising a liquid permeable carrier layer, a detection layer and an outer layer, wherein the liquid permeable carrier layer is water permeable, and the upper surface thereof is used for carrying biological samples, such as feces, Urine or biological secretions, the detection layer is translucent and water permeable, and contains a detection substance uniformly distributed in at least one region, the outer layer is impervious to water and translucent, and is attached to liquid permeability The lower surface of the carrier layer and the lower surface of the detection layer. Further, one side of the liquid permeable carrier layer is a corresponding side of the connection detecting layer, and the upper surface of the junction of the liquid permeable carrier layer and the detecting layer forms at least one folding line.

Specifically, the biological sample is infiltrated into the detection layer after the at least one folding line is bent, and reacts with the detection substance, and the biological sample can be identified as whether or not the occult blood is contained by the reaction.

Another object of the present invention is to provide a occult blood test paper comprising a liquid permeable carrier layer, a detection layer and an outer surface layer which are sequentially stacked from top to bottom, wherein the liquid permeable carrier layer has water permeability and the liquid permeability multiplication The upper surface of the carrier layer is for carrying the biological sample, and at least one folding line is disposed at the middle of the upper surface for bending, and the detecting layer is translucent and water permeable, and the upper surface of the detecting layer is attached. On the lower surface of the liquid permeable carrier layer, and the detection layer is provided with at least one cavity, each cavity having at least two grooves for accommodating the detection substance, separated by a separation film, and the separation film is Aligned with the folding line in the vertical direction, the detecting substance comprises a coloring substance and a redox substance, which are respectively accommodated in different groove cells, and further, the outer surface layer is water permeable and translucent, and is a dura mater structure. And attached to the lower surface of the detection layer.

The redox species has a function of decomposing and releasing free electrons by redox reaction of iron, and the coloring matter has a function of receiving free electrons released by the redox species to cause a color change of the coloring matter.

The biological sample is sandwiched by the upper surface of the liquid permeable carrier layer and penetrated into the detection layer after the folding line is bent. The separation film breaks when the folding line is bent to release the coloring matter and the redox substance contained in the groove, and reacts with the biological sample to react, thereby identifying whether the biological sample contains occult blood by whether the reaction is discolored or not.

Still another object of the present invention is to provide a occult blood test paper comprising a liquid permeable carrier layer, a detection layer and an outer surface layer which are sequentially stacked from top to bottom, wherein the liquid permeable carrier layer has water permeability and liquid permeability multiplication The upper surface of the carrier layer is for carrying the biological sample, and at least one folding line is disposed at the middle of the upper surface for bending, and the detecting layer is translucent and water permeable, and the upper surface of the detecting layer is attached. On the lower surface of the liquid permeable carrier layer, and the detecting layer is provided with at least one cavity, each cavity having at least two slots for accommodating the detecting substance, wherein the at least two slots are respectively arranged in the vertical direction The detection material is located on two sides of the folding line, and the detecting substance comprises a coloring substance and a redox substance, which are respectively accommodated in different troughs, and the detecting layer is disposed with a plurality of connecting channels which do not intersect each other, and each connecting channel is composed of phases. The corresponding cavity is formed to extend toward the folding line. Further, the outer layer is water permeable and translucent, and has a hard film structure and is attached to the lower surface of the detecting layer.

The redox species has a function of decomposing and releasing free electrons by redox reaction of iron, and the coloring matter has a function of receiving free electrons released by the redox species to cause a color change of the coloring matter.

The biological sample is sandwiched by the upper surface of the liquid permeable carrier layer and penetrated into the detection layer after the folding line is bent. When the folding line is bent, the at least two slots are accommodated The coloring matter and the redox species are released and forced to flow out and concentrate in the vicinity of the folding line, and react with the biological sample to react, and whether the biological sample contains occult blood can be identified by whether the reaction is discolored or not.

The length of the connecting passage may be configured not to span or cross the folding line, and if the connecting passage is to span the folding line, each connecting passage is further provided with at least one notch structure configured to be vertically aligned with the folding The function of the notch structure is to rupture when the folding line is bent, so that the coloring matter and the redox substance in the at least two troughs flow out through the notch structure, and are in contact with the biological sample that penetrates into the vicinity of the notch structure and reaction.

Therefore, the structure of the invention is simple and convenient to use and operate, and does not require any pre-treatment or post-treatment of the biological sample, in particular, does not cause any discomfort to the user, is quite hygienic, and can greatly improve the overall efficiency of detecting occult blood. It is very suitable for the general public in the home environment to complete the individual occult blood test without the assistance of professional medical personnel, so the industrial utilization is quite high and competitive in the market.

10‧‧‧ occult blood test strip

12‧‧‧ liquid-permeable carrier layer

121‧‧‧Folding line

14‧‧‧Detection layer

141‧‧‧Test substances

1411‧‧‧Coloring matter

1412‧‧‧redox species

15‧‧‧ cavity

16‧‧‧Slot

17‧‧‧Separate film

18‧‧‧ Linkage

181‧‧‧ gap structure

24‧‧‧Outer layer

30‧‧‧ Biological samples

The first figure shows a schematic view of a occult blood test paper according to a first embodiment of the present invention.

The second figure shows a schematic view of a occult blood test paper according to a second embodiment of the present invention.

The third figure shows a schematic view of a occult blood test paper according to a third embodiment of the present invention.

The fourth figure shows a schematic view of a occult blood test paper according to a fourth embodiment of the present invention.

The embodiments of the present invention will be described in more detail below with reference to the drawings and the reference numerals, which can be implemented by those skilled in the art after having studied this specification.

Please refer to the first figure for a schematic diagram of the occult blood test paper of the first embodiment of the present invention. Such as the first As shown in the figure, the occult blood test strip 10 of the first embodiment of the present invention comprises a liquid permeable carrier layer 12, a detecting layer 14 and an outer skin layer 24, wherein the lower surface of the liquid permeable carrier layer 12 and the lower surface of the detecting layer 14 It is disposed to be attached to the upper surface of the outer skin layer 24, and one side of the liquid-permeable carrier layer 12 is a corresponding side of the connection detecting layer 14, and further, the liquid-permeable carrier layer 12 and the detecting layer 14 are The upper surface of the joint forms at least one fold line 121, but only a single fold line 121 is shown for convenience of explanation. The upper surface of the liquid permeable carrier layer 12 is used to carry a biological sample 30, such as feces, urine or biological secretions.

Specifically, the liquid permeable carrier layer 12 is water permeable, so that the biological sample 30 is easily adhered and adhered, and may be composed of at least one of chemical fiber, plant fiber, animal fiber structure, and mineral fiber, wherein the chemical fiber At least one of polyester, nylon, spandex, acrylic, acetate, the animal fiber comprises at least one of animal hair, silk fiber and spider fiber, and the mineral fiber comprises asbestos.

The detection layer 14 includes a detection substance 141 uniformly distributed in at least one area, and only the distribution is shown in the middle area, and can be used to react after contacting the biological sample 30, and whether the biological sample 30 is contained by whether or not discoloration occurs after the reaction. The occult blood reaches the function of detecting whether the biological sample 30 contains occult blood. For example, if the biological sample 30 containing occult blood is feces, it indicates that colorectal cancer may have occurred. Further, the detection layer 14 is translucent and may be composed of at least one of plant fiber, nitrocellulose, polyethylene, polypropylene, cellulose acetate, polyvinyl chloride, and polyvinylidene chloride.

Since the folding line 121 is located at the junction of the liquid-permeable carrier layer 12 and the detecting layer 14, the liquid-permeable carrier layer 12 and the detecting layer 14 can be easily folded and contacted with each other. The biological sample 30 on the carrier layer 12 can be reacted by contacting the detection substance 141 of the detection layer 14.

The outer skin layer 24 can be used to prevent the user from coming into contact with the biological sample, improve the hygienic use during use, and provide appropriate mechanical strength to prevent the occult blood test strip 10 from being bent due to misuse. For example, when a certain force is applied, the outer surface layer 24 is Will bend. Further, the outer skin layer 24 has light transmissivity, and particularly has water impermeability. Therefore, the user can see through the lower surface of the outer skin layer 24 whether or not discoloration occurs after the biological sample 30 contacts the detecting substance 141. Another function of the outer skin 24 is to improve ease of use and hygiene, as the user can hold the lower surface of the outer skin 24 and wipe the upper surface of the liquid permeable carrier layer 12 to the biological sample 30 in a wiping manner. And then applying force to the lower surface of the outer skin layer 24 to align the liquid-permeable carrier layer 12 and the detection layer 14 along the fold line 121 such that the biological sample 30 is sandwiched between the liquid-permeable carrier layer 12 and the detection layer 14 The closure is sealed to prevent exposure. Further, the outer skin 24 is impervious to water, so that the biological sample 30 does not penetrate the lower surface of the outer skin 24 and stains the hand to be used, thereby solving any possible discomfort.

Referring to the second figure, a schematic diagram of a occult blood test paper according to a second embodiment of the present invention, wherein the left side view of the second figure is a top view of the second embodiment occult blood test paper, and the right side view is a side view of the occult blood test paper of the second embodiment. Cutaway view. As shown in the second figure, the occult blood test strip 10 of the second embodiment comprises a liquid permeable carrier layer 12, a detection layer 14 and an outer skin layer 24, and has characteristics similar to those of the first embodiment, such as a liquid permeable carrier layer. The upper surface of 12 is also used to carry the biological sample 30, but the difference therebetween includes that the liquid-permeable carrier layer 12, the detection layer 14 and the outer layer 24 of the second embodiment are sequentially stacked from top to bottom, and The liquid permeable carrier layer 12 and the detection layer 14 are not connected side by side and disposed on the outer surface layer 24. Further, the upper surface of the liquid permeable carrier layer 12 has at least one folding line 121, such as a liquid permeable load in the figure. The middle portion of layer 12, but only a single fold line 121 is shown for ease of illustration.

More specifically, the detection layer 14 of the second embodiment has at least one cavity 15, However, only a single cavity 15 is shown in the figure for convenience of description, and each cavity 15 has at least two slots 16 for accommodating the detecting substance 141, such as the left side and the right side of the folding line 121, and the at least two slots 16 is separated by a separation film 17, wherein the separation film 17 is aligned with the folding line 121 in the vertical direction. It should be noted that only two cells 16 and a single separation film 17 are shown in the figure, that is, a plurality of separation films 17 may be included to separate adjacent cells 16, such as in an array or a circle. These slots 16 are arranged and arranged.

Further, the detecting substance 141 of the second embodiment includes the coloring matter 1411 and the redox substance 1412, and is accommodated in the groove 16 on the left side and the groove 16 on the right side, respectively. The redox species 1412 decomposes with iron in the biological sample 30 (such as hemoglobin iron in the blood), and releases free electrons, causing the color of the coloring matter 1411 to change to achieve discoloration. Therefore, if the biological sample 30 undergoes a color change upon contact with the detection substance 141, it means that the biological sample 30 contains a blood component, that is, contains occult blood.

Preferably, the coloring matter 1411 may comprise at least one of toluidine, phenolphthalein, piramid, guaiac, guaiac gum, tetramethylaniline, and an organic alcohol, and the redox species 1412 may Containing at least one of hydrogen peroxide and sodium hydrogencarbonate.

In practical use, the user can hold the outer skin 24, such as the left and right sides of the folding line 121, and then apply the force to the lower surface of the outer layer 24 to bend the entire occult blood test paper 10 along the folding line 121, so that the liquid permeable The left side portion and the right side portion of the upper surface of the sexual load layer 12 located at the folding line 121 are folded into contact with each other. At this time, the biological sample 30 is enclosed and closed, and the left groove 16 and the right groove 16 are pressed against each other under the above-mentioned force, so that the separation film 17 is broken, and then released and mixed and accommodated on the left side. The coloring matter 1411 and the redox species 1412 in the trough 16 and the trough 16 on the right side. At the same time, the biological sample 30 also permeates the liquid permeability multiplication under the same force. The carrier layer 12 reaches the detection layer 14 and is in contact with the mixture of the coloring matter 1411 and the redox species 1412 in the detection layer 14. If the reaction of the biological sample 30 with the coloring matter 1411 and the redox substance 1412 causes discoloration, it indicates that the biological sample 30 has occult blood.

Referring to the third figure, a schematic view of a occult blood test paper according to a third embodiment of the present invention, wherein the left side view is a top view of the third embodiment occult blood test paper, and the right side view is a side cross-sectional view of the third embodiment occult blood test paper. As shown in the third figure, the occult blood test strip 10 of the third embodiment comprises a liquid permeable carrier layer 12, a detection layer 14 and an outer skin layer 24 which are sequentially stacked from top to bottom, similar to the second embodiment, wherein The upper surface of the liquid carrier layer 12 carries the biological sample 30, and the intermediate portion of the upper surface of the liquid-permeable carrier layer 12 is provided with at least one fold line 121, but only a single fold line 121 is shown, and the detection layer 14 is There are at least two cavities 15 on both sides of the folding line 121, and each cavity 15 has at least two slots 16 for accommodating the coloring matter 1411 and the redox substance 1412, respectively.

However, the third embodiment of the present invention differs from the second embodiment in that it does not have the separation film 17, and each cavity 15 is spaced apart from each other without being adjacent, and the at least two cells 15 are vertically separated. It is configured to be located on two sides of the folding line 121, such as the left side and the right side in the figure. In particular, the detecting layer 14 is also provided with a plurality of connecting channels 18 that do not intersect each other, and each connecting channel 18 is folded by a certain cavity 15 The line 121 is formed to extend, wherein the length of the connecting passage 18 is configured not to cross the folding line 121. Therefore, when the user bends and bends the lower surface of the outer surface layer 24, the occult blood test strip 10 is folded in half along the folding line 121, and the biological sample 30 is sandwiched by the liquid permeable carrier layer 12, and the force is also applied. Squeezing into the trough 16 on both sides of the folding line 121 forces the coloring matter 1411 and the redox substance 1412 therein to flow out to the vicinity of the folding line 121, so the biological sample 30 will permeate through the liquid permeating force. The carrier layer 12 is brought into the detection layer 14 and reacted with a mixture of the coloring matter 1411 and the redox species 1412. If the discoloration occurs after the reaction, it means the biological sample This 30 contains occult blood.

Further, referring to the fourth figure, a schematic view of the occult blood test paper of the fourth embodiment of the present invention is similar to the third embodiment, and the difference is that the length of the connecting passage 18 of the fourth embodiment is configured to span the folding line 121, and each The connecting passages 18 are also provided with at least one notch structure 181 which is arranged to be aligned with the folding line 121 in the vertical direction. Therefore, when the user bends the occult blood test strip 10, the notch structure 181 of the connecting channel 18 is broken, so that the coloring matter 1411 and the redox substance 1412 in the trough 16 flow out through the notch structure 181 to be mixed, and the infiltration The biological sample 30 near the gap structure 181 is contacted and reacted, and whether the biological sample 30 contains occult blood is recognized by whether or not discoloration occurs after the reaction.

Further, the fourth embodiment is characterized in that the slot 16 can be disposed in a position close to or aligned with the finger in accordance with the action of the hand-held operation, so that the finger can effectively squeeze when the force is bent and the occult blood test paper 10 is bent. The trough 16 forces the coloring matter 1411 and the redox substance 1412 therein to flow out of the notch structure 181, and the troughs 16 on the two sides of the folding line 121 can be spaced apart by an appropriate distance to fit the hand-held position, which is convenient for operation, because the two sides When the distance between the cells 16 is too short or too long, such as being too concentrated or away from the folding line 121, the fingers may not be effectively applied to the cells 16 during the folding, resulting in the coloring matter 1411 and the redox substance 1412. The squeezing effect is not good, which seriously affects the efficacy of detecting occult blood.

It should be noted that the detecting layer 14 in the second, third, and fourth embodiments can directly replace the detecting layer 14 of the first embodiment, that is, the detecting layer 14 of the first embodiment can also utilize the cavity 15 The slot 16 contains a means for detecting the substance 141 in place of the technical feature in which the detecting substance 141 in the first figure is uniformly distributed on the detecting layer 14.

In summary, the invention is characterized in that the folding line is used to facilitate the user to fold The entire occult blood test paper is such that the biological sample thereon is clamped and kept hygienic, and at the same time, the biological sample is infiltrated and reacted with the test substance under the pressing force at the time of folding, and the outer surface under the occult blood test paper is translucent. Therefore, the user can see through the lower surface of the outer surface layer whether the reaction of the biological sample contacts the detection substance is discolored, and whether the biological sample contains occult blood, and if the biological sample is feces, it indicates the possibility of colorectal cancer. Therefore, the occult blood test strip of the present invention provides a very convenient occult blood test function, and the occult blood can be identified by wiping and folding, without any pre-treatment or post-treatment of the biological sample, especially without causing any discomfort to the user, and health.

Another feature of the present invention is that the overall operation and operation is simple, and the general public in a home environment can perform individual occult blood testing without the assistance of professional medical personnel, so that the industrial utilization is quite high and the market is competitive.

The above is only a preferred embodiment for explaining the present invention, and is not intended to limit the present invention in any way, and any modifications or alterations to the present invention made in the spirit of the same invention. All should still be included in the scope of the intention of the present invention.

Claims (10)

A occult blood test paper comprising: a liquid permeable carrier layer, having water permeability, and having an upper surface and a lower surface, wherein the upper surface is for carrying a biological sample; and the detecting layer is translucent and permeable to water And having an upper surface and a lower surface, comprising a detecting substance uniformly distributed in at least one area; and an outer surface layer having water impermeability and light transmissivity, and being attached to the liquid permeable load a lower surface of the layer and a lower surface of the detecting layer, wherein one side of the liquid-permeable carrier layer is a corresponding side connecting the detecting layer, and the liquid-permeable carrier layer and one of the detecting layer An upper surface of the joint forms at least one folding line, and the biological sample penetrates into the detecting layer after being bent, and reacts with the detecting substance to perform a reaction, and whether the reaction is discolored by the reaction And identifying whether the biological sample contains occult blood, the biological sample containing feces, and the occult blood test paper is used for a user to hold the lower surface of the outer layer, and wiping the upper surface of the liquid permeable carrier layer Stick to the creature Present, and force the lower surface of the outer skin layer, the fold line along which the liquid-ride off the transparent layer and the detection layer. The occult blood test paper according to claim 1, wherein the liquid permeable carrier layer is composed of at least one of chemical fiber, plant fiber, animal fiber structure and mineral fiber, and the detection layer is made of plant fiber and nitrification. At least one of fibers, polyethylene, polypropylene, cellulose acetate, polyvinyl chloride, and polyvinylidene chloride. The occult blood test paper according to claim 2, wherein the chemical fiber comprises at least one of polyester, nylon, spandex, acrylic, and acetate fiber, and the animal fiber comprises at least one of animal hair, silk fiber, and spider fiber. In one of them, the mineral fiber contains asbestos. A occult blood test paper comprising: a liquid permeable carrier layer, is water permeable, and has an upper surface and a lower surface, wherein the upper surface is for carrying a biological sample, and at least a middle portion of the upper surface is disposed at least a folding line for a detection layer, which is translucent and water permeable, and has an upper surface and a lower surface, the upper surface of the detection layer is attached to the lower surface of the liquid permeable carrier layer, and the detection layer Having at least one cavity, each cavity having at least two slots for accommodating a detection substance, the at least two slots being separated by a separation film, the separation film being vertically aligned with The folding line, the detecting substance comprises a coloring substance and a redox substance, which are respectively accommodated in different troughs; and an outer surface layer which is water permeable and translucent, and is attached to the detecting a lower surface of the layer, wherein the redox species has a function of decomposing and releasing free electrons by performing a redox reaction on iron, and the coloring matter has a free electron released by receiving the redox species to cause the coloring matter The color produces a function of transforming the biological sample by being clamped by the upper surface of the liquid-permeable carrier layer and penetrating into the detection layer after the folding line is bent, and the separation film is at the folding line Cracked when bent And releasing the coloring matter and the redox substance contained in the at least two cells, and reacting with the biological sample, and identifying whether the biological sample contains occult blood by the color change of the reaction, the biological sample comprises Feces, and the occult blood test paper is used for a user to hold the lower surface of the outer layer, and the upper surface of the liquid permeable carrier layer is adhered to the biological sample by wiping, and is applied to the The liquid permeable carrier layer and the detection layer are folded in half along the fold line under the lower surface of the outer layer. The occult blood test paper according to claim 4, wherein the liquid permeable carrier layer is composed of at least one of chemical fiber, plant fiber, animal fiber structure and mineral fiber, and the chemical fiber comprises polyester, nylon, At least one of spandex, acrylic, acetate fiber, the animal fiber comprising at least one of animal hair, silk fiber and spider fiber, the mineral fiber comprising asbestos, and the detection layer being plant fiber, nitrocellulose, At least one of polyethylene, polypropylene, cellulose acetate, polyvinyl chloride, and polyvinylidene chloride is used. The occult blood test paper according to item 5 of the patent application scope, wherein the iron substance contained in the biological sample is hemoglobin iron in blood, and the redox substance comprises hydrogen peroxide and sodium hydrogen hydride to One of the lesser ones, and the coloring matter comprises at least one of toluidine, phenolphthalein, piramid, guaiac, guaiac gum, tetramethylaniline, and an organic alcohol. A occult blood test paper comprising: a liquid permeable carrier layer, is water permeable, and has an upper surface and a lower surface, wherein the upper surface is for carrying a biological sample, and at least a middle portion of the upper surface is disposed at least a folding line for bending; a detecting layer, translucent and water permeable, having an upper surface and a lower surface, the upper surface of the detecting layer being attached to the liquid permeable carrier layer a surface, and the detecting layer is provided with at least one cavity, each cavity having at least two slots for accommodating a detecting substance, wherein the at least two slots are respectively disposed in the vertical direction to be located at the folding line On the side, the detecting substance comprises a coloring substance and a redox substance, which are respectively accommodated in different troughs, and the detecting layer is provided with a plurality of connecting channels which do not intersect each other, and each connecting channel is correspondingly Forming a cavity extending toward the folding line; and an outer skin layer having water permeability and light transmissivity and attached to a lower surface of the detecting layer, wherein the redox substance has a redox reaction on iron Decomposed and released from a function of an electron, and the coloring matter has a function of receiving free electrons released by the redox species to cause a color change of the coloring matter, the biological sample being liquid-permeable after the folding line is bent The upper surface of the carrier layer is sandwiched and penetrated into the detection layer, and when the folding line is bent, the coloring matter and the redox substance contained in the at least two slots are released Forcing out and concentrating to the vicinity of the folding line, and reacting with the biological sample to identify whether the biological sample contains occult blood by discoloration of the reaction, the biological sample containing feces, and the occult blood test paper is used for one The user holds the lower surface of the outer layer and wipes it The method is such that the upper surface of the liquid permeable carrier layer is adhered to the biological sample, and under the lower surface of the outer layer, the liquid permeable carrier layer and the detection layer are folded in half along the folding line. The occult blood test paper according to claim 7 , wherein the liquid permeable carrier layer is composed of at least one of chemical fiber, plant fiber, animal fiber structure and mineral fiber, and the chemical fiber comprises polyester, nylon, At least one of spandex, acrylic, acetate fiber, the animal fiber comprising at least one of animal hair, silk fiber and spider fiber, the mineral fiber comprising asbestos, and the detection layer being plant fiber, nitrocellulose, At least one of polyethylene, polypropylene, cellulose acetate, polyvinyl chloride, and polyvinylidene chloride, the iron contained in the biological sample is hemoglobin iron in blood, and the redox substance comprises hydrogen peroxide And at least one of sodium percarbonate comprising at least one of toluidine, phenolphthalein, piramid, guaiac, guaiac gum, tetramethylaniline, and an organic alcohol . The occult blood test strip according to item 7 of the patent application, wherein the length of the connecting passage is configured not to cross the folding line. The occult blood test strip according to claim 7 , wherein the length of the connecting passage is configured to span the folding line, and the connecting passage is provided with at least one notch structure disposed to be vertically aligned with the folding line. The at least one notch structure is broken when the folding line is bent, so that the coloring matter and the redox substance in the at least two grooves flow out through the at least one notch structure, and penetrates into the at least notch structure The nearby biological sample is in contact with and reacts.