TWI482964B - Method and device for measuring hematocrit (hct) - Google Patents

Description

Hematocrit detection method and detection system thereof

The invention relates to a detection method and a system thereof, in particular to a method for detecting hematocrit (Hct) in blood and a detection system using the same.

Hematocrit (Hct), also known as the percentage of blood volume, refers to the proportion of red blood cells contained in a certain amount of blood, and is often used as an indicator for diagnosing anemia or cardiovascular disease. However, for the measurement of blood glucose level, hematocrit is often one of the key factors affecting the blood glucose measurement. Therefore, in order to accurately measure the blood glucose level, it is often necessary to measure the blood specific volume of the subject first, and then according to The hematocrit is corrected for blood glucose measurements.

Traditional blood specific capacity tests mostly use manual or special machines (such as blood cell counting machines). The former has the disadvantages of complicated operation and time consuming, while the latter requires high cost disadvantages of procurement and maintenance.

Therefore, is there a method or mechanism that can improve the accuracy and reliability of blood specific volume measurement, and has a simple operation mode for the subject to perform measurement operations.

According to one aspect of the present invention, there is provided a method for detecting hematocrit (Hct) in blood and a detecting device using the same.

According to an embodiment of the present invention, a blood specific volume detecting method includes Applying a blood to a detecting electrode, applying a voltage value to the pair of detecting electrodes to obtain a response current value, and finally obtaining a hematocrit of the blood according to a predetermined interpretation rule and the response current value.

According to another embodiment of the invention, the hematocrit detection system includes a detection unit and a detection device. The detecting unit includes a pair of detecting electrodes having a receiving portion and a contact portion. The receiving portion is for receiving a blood. The detecting device is connected to the contact portion of the pair of detecting electrodes, and applies a voltage value to the contact portion according to a predetermined determining rule to obtain a blood specific volume of the blood.

Therefore, compared with the conventional application of the electrode test piece, the blood specific capacity measurement disclosed in the present invention can improve the accuracy and reliability in addition to the simple operation.

Please refer to FIG. 1 , which is a schematic diagram of an embodiment of a hematocrit detection system of the present invention. The hematocrit detection system includes a detection unit 10 and a detection device 20. The detecting unit 10 includes a pair of detecting electrodes 110 having a receiving portion 111 and a contact portion 112 for receiving a blood. The detecting device 20 is connected to the contact portion 112 of the pair of detecting electrodes 110, and applies a voltage value to the contact portion 112 according to a predetermined interpretation rule to obtain the hematocrit of the blood.

The detecting device 20 includes a pair of ports 210, a reference voltage source 220 and a controller 230. One of the first ends of each of the ports 210 is connected to the contact portion 112, and the reference voltage source 220 and one of the A second end of the port 210 is connected to provide the voltage value required for detection. The controller 230 is connected to the second terminal of the other connection port 210 and the reference voltage source 220, and receives the response current value of the detection unit 10 to obtain the blood specific volume of the blood.

2 to 4, FIG. 2 is an exploded perspective view showing an embodiment of a detecting unit in the blood specific volume detecting system of the present invention, and FIG. 3 is another embodiment of the detecting unit in the blood specific volume detecting system of the present invention. FIG. 4 is a schematic view showing an embodiment of a detecting unit in the blood specific volume detecting system of the present invention. In an embodiment, as shown in FIG. 3 , the detecting unit 10 can be a test piece design, and includes a substrate 100 , the pair of detecting electrodes 110 , a partition plate 130 , and a cover plate 140 . The pair of detecting electrodes 110 are disposed on the substrate 100. The spacer 130 is disposed on the substrate 100 and partially covers the pair of detecting electrodes 110 such that the end electrode portions of the pair of detecting electrodes 110 are exposed and included in an opening 131. The opening 131 is formed at a front end of the partition plate 130 such that the pair of detecting electrodes 110 are exposed corresponding to the front end electrode portion of the opening 131. The interface 120 covers the position of the opening 131 of the pair of detecting electrodes 110 corresponding to the spacer 130.

Here, the receiving portion 111 of the pair of detecting electrodes 110 is defined as a region exposed at the position of the opening 131, and the contact portion 112 is defined as a rear end electrode of the pair of detecting electrodes 110 not covered by the spacer 130.

In another embodiment, as shown in FIG. 4, the detecting unit 10 further includes an surfactant 120 disposed on the substrate 100 and covering the receiving portion of the detecting electrode 110. 111.

The cover plate 140 is disposed on the partition plate 130 and includes a guide port 141 and a guide hole 142. The guide port 141 is disposed at a front end of the cover plate 140 and overlaps with the opening 131 of the partition plate 130. The guide hole 142 is defined in the position of the cover plate 140 corresponding to the opening 131 of the partition plate 130, and the opening 131 A conduction path is formed.

According to an embodiment, the substrate 100 is an insulating substrate, and the material thereof is not particularly limited, and may be polyethylene terephthalate (PET), polyvinyl chloride (PVC), or glass fiberboard (FR-4). ), non-conductive materials such as polycarbonate (PC), polyethylene (PE), polypropylene (PP), polystyrene (PS), polyester (Polyester sulphone), ceramic plate (CEM), and glass. The material of the spacer 130 and the cover 140 is not particularly limited, and the same material as that of the substrate 100 can be used.

According to an embodiment, the pair of detecting electrodes 110 is a conductive material, but is not limited to a metal material. The pair of detecting electrodes 110 can form any electrode pattern on the substrate 100 by a sputtering method, an evaporation method, or a screen printing method. As shown in FIG. 2, the electrode patterns of the pair of detecting electrodes 110 are oppositely disposed on the two L-shaped electrodes, and the two short sides thereof are disposed in parallel with each other at the front end of the substrate 100, and the two long sides thereof are disposed in parallel with each other on the substrate 100. And extending rearward to the rear end of the substrate 100.

When a user takes blood through a special lancet, blood is dripped in the guide hole 142, and the conduction path formed by the guide hole 142 and the opening 131 of the spacer 130 generates a capillary phenomenon for allowing blood to pass through. The opening 131 is introduced into the detecting unit 10 and covered with the interface active The pair of detecting electrodes 110 of the agent 120 are in contact. Next, the contact portion 112 of the pair of detecting electrodes 110 is connected to the pair of ports 210 of the detecting device 20, and then the detecting device 20 is activated.

The controller 230 drives the reference voltage source 220 to apply a voltage value between the pair of detecting electrodes 110, and the applied voltage is attached to the blood of the surfactant 120 to generate an electrochemical reaction, thereby forming a response. The conduction path of the current whose response current value changes due to the hematocrit of the blood. The controller 230 reads the response current value to determine the hematocrit of the blood to be measured. The controller 230 can distinguish the hematocrit of the blood for different response current values in one unit time.

Please refer to FIG. 5A to FIG. 5C. FIG. 5A to FIG. 5C are respectively schematic diagrams showing changes in response current versus time in different embodiments when a voltage is applied to the detecting unit by the detecting device. As shown in Fig. 5A, the 40% hematocrit response current value of the unadded surfactant 120 is higher than 50% hematocrit, 50% higher than 60%, 60% higher than 70%, 70% high. At 90%, the higher the hematocrit percentage, the slower the response current changes. Similarly, as shown in the example of FIG. 5B, the response specific current value of the hematocrit of 41% of the surfactant 120 is higher than the hematocrit of 60%. As shown in Fig. 5C, the graph shows the response current versus time for 38%, 69%, and 75%, respectively.

It is worth noting that since the surfactant 120 has both hydrophilic and hydrophobic properties, the application of the surfactant 120 allows the substances in the blood to be homogenized and dispersed in the plasma to make the measurement stable.

In one embodiment, the surfactant 120 is selected from the group consisting of bromination Cetyltrimethylammonium bromide (CTAB), octylphenol polyoxyethylene alcohol-X100 (Triton X-100), polysorbate (TWEEN) 20, polysorbate (TWEEN) 40, poly Sorbitol ester (TWEEN) 60, sorbitan monosulphate (Span) 20, Carboxymethyl cellulose (CMC), sodium cholate and sodium decanoate (Sodium Dodecyl Sulphate, SDS) ) the group consisting of.

In addition, in an embodiment, the voltage value applied to the pair of detecting electrodes 110 may be between 1 and 3 volts, and the unit time may be between 0.01 and 1 second. In order for the controller 230 to perform the hematocrit interpretation on the received response current value efficiently and accurately, the controller 230 needs to perform (for example, through an electrochemical device) to set the blood ratio of different percentage values. The data of the capacity is pre-stored in the controller 230 for conversion of each detected current.

Please refer to FIG. 6, which is a flow chart of an embodiment of the blood specific volume detecting method of the present invention. According to the blood specific volume detecting system, the blood specific volume detecting method includes the steps of: applying a blood to a pair of detecting electrodes; S12 applying a voltage value to the pair of detecting electrodes to obtain a response current value; and S14 A blood specific volume of the blood is obtained according to a preset interpretation rule and the response current value.

Wherein, the blood is applied to the pair of detecting electrodes as described in step S10, and the difference of the visible detecting electrodes is different, according to another implementation. For example, the blood is applied to the pair of detection electrodes coated with an surfactant.

The preset interpretation rule includes a plurality of hematocrit data, which are the relationship between the hematocrit and the amount of change in the response current value at different voltage values, and thereby determining the hematocrit of the blood to be measured.

Therefore, the blood specific capacity measurement of the present invention is higher than that of the conventional application electrode test piece, so that the measurement of the hematocrit can improve the accuracy and reliability in addition to the simple operation.

However, the above-mentioned preferred embodiments of the present invention are not intended to limit the scope of the invention, and any changes and modifications apparent to those skilled in the art should be considered as not The substance of the invention is removed.

10‧‧‧Detection unit

100‧‧‧Substrate

110‧‧‧Detection electrode

111‧‧‧ Receiving Department

112‧‧‧Contacts

120‧‧‧Interfacial active agent

130‧‧‧Baffle

131‧‧‧ openings

140‧‧‧ cover

141‧‧ ‧ guide

142‧‧‧ Guide hole

20‧‧‧Detection device

210‧‧‧Connector

220‧‧‧reference voltage source

230‧‧‧ Controller

Figure 1 is a schematic illustration of one embodiment of a hematocrit detection system of the present invention.

Fig. 2 is an exploded perspective view showing an embodiment of a detecting unit in the blood specific volume detecting system of the present invention.

Fig. 3 is an exploded perspective view showing an embodiment of a detecting unit in the blood specific volume detecting system of the present invention.

Fig. 4 is a schematic view showing an embodiment of a detecting unit in the blood specific volume detecting system of the present invention.

Fig. 5A is a schematic diagram showing changes in response current versus time when a voltage is applied to the detecting unit by the detecting device.

FIG. 5B is a schematic diagram showing changes in response current versus time when a voltage is applied to the detecting unit by the detecting device.

Fig. 5C is a schematic diagram showing changes in response current versus time when a voltage is applied to the detecting unit by the detecting device.

Figure 6 is a flow chart of the blood specific volume detecting method of the present invention.

Claims (11)

A blood specific volume detecting method includes: applying a blood to a pair of detecting electrodes; applying a voltage value to the pair of detecting electrodes to obtain a response current value; and obtaining according to a preset interpretation rule and the response current value One of the hematocrit of the blood; wherein the preset interpretation rule compares the response current value with a plurality of blood specific volume data stored in advance to determine the hematocrit corresponding to the response current value. The method of detecting according to claim 1, wherein the blood system is applied to the pair of detecting electrodes coated with an surfactant. The detection method according to claim 2, wherein the surfactant is selected from the group consisting of cetyltrimethylammonium bromide, octylphenol polyoxyethylene alcohol-X100, polysorbate 20 A group consisting of polysorbate 40, polysorbate 60, sorbitan monolaurate 20, carboxymethylcellulose, sodium cholate and sodium laurate sulfate. The detection method of claim 1, wherein the voltage value is between 1 and 3 volts (Volts). A blood specific volume detecting system comprising: a detecting unit comprising a pair having a receiving portion and a contact a detecting electrode, wherein the receiving portion is configured to receive a blood; and a detecting device is connected to the contact portion of the pair of detecting electrodes, and applying a voltage value to the contact portion according to a predetermined determining rule to obtain a blood specific volume of the blood; wherein the detecting device pre-stores a plurality of hematocrit data, the preset determining rule is to compare the response current value with each of the hematocrit data to determine that the corresponding The hematocrit of the response current value. The detection system of claim 5, wherein the detecting device further comprises: a pair of connecting ports, one of the first ends of each of the connecting ports is connected to the contact portion; a reference voltage source is associated with one of a second end of the connection port is connected to provide the voltage value; and a controller is connected to the second end of the other connection port and the reference voltage source, and receives the response current value The hematocrit of the blood is obtained. The detection system of claim 6, wherein the detecting unit further comprises: a substrate for the pair of detecting electrodes disposed thereon; a spacer disposed on the substrate, partially covering the And the detection electrode is such that the end electrode portion of the pair of detection electrodes is exposed, and an opening is formed at a front end of the spacer, so that the pair of detection electrodes correspond to The front end electrode portion of the opening is exposed; and a cover plate is disposed on the partition plate, and includes: a guide port disposed at a front end of the cover plate and overlapping with the opening of the partition plate; and a guide hole Opening a cover corresponding to the opening position of the partition, forming a conduction path with the opening. The detection system of claim 7, wherein the substrate is an insulating substrate, and the substrate, the separator and the cover are made of polyethylene terephthalate (PET) and polychlorinated Ethylene (PVC), glass fiber reinforced plastic (FR-4), polycarbonate (PC), polyethylene (PE), polypropylene (PP), polystyrene (PS), polyester (Polyester sulphone), ceramic plate ( CEM) or glass. The detection system of claim 5, wherein the detecting unit further comprises an intervening agent covering the receiving portion of the pair of detecting electrodes. The detection system of claim 9, wherein the surfactant is selected from the group consisting of cetyltrimethylammonium bromide, octylphenol polyoxyethylene alcohol-X100, polysorbate 20 A group consisting of polysorbate 40, polysorbate 60, sorbitan monolaurate 20, carboxymethylcellulose, sodium cholate and sodium laurate sulfate. The detection system of claim 5, wherein the voltage value is between 1 and 3 volts.