1314648 九、發明說明： 【發明所屬之技術領域】 本發明是有關於一種生物晶片，且特 β 行⑴疋有關於一種 生化反應檢測卡匣及其分析方法。 裡 【先前技術】1314648 IX. Description of the Invention: [Technical Field] The present invention relates to a biochip, and the specific β-ray (1) is related to a biochemical reaction detection cassette and an analysis method thereof. [Prior Art]
隨著預防醫學於全球各地風行，預防重於治療々 也慢慢深植於所有人心中，因為早期發現早期治療不僅; 以大幅提升治癒的機會，亦可減輕後續衍生的龐大醫療負 擔’對個人或社會都有極正面的助益’這也是為何定_ 康檢查的實施在世界各先進國家都相當受到重視且大力相 行的主要原因。然而’大型檢測儀雖然可以非常靈敏的过 行許多生理功能指標的檢測，卻往往無法達到少量樣品、 快速分析與隨身檢測的目標。以新陳代謝相關的疾病為 例’雖然傳統的全自動生化分析儀可以常規的檢測碳氫u 合物、氨基酸、維他命或其他小分子量的化合物，但由灰 體積龐大且價格昂貴，因此？半必須親臨醫療機構才得〇 進行檢測，除需護理人員抽取5— 1〇“的血液外，分析^ 作更需專業的醫檢人員才能完成，檢驗報告回報時担 (，n^r〇Und-time)也相當長，完全無法滿足醫療照護端之$ 時檢知或一般民眾隨時自我檢查的要求。 為因應個人自主健康意識的逐漸抬頭，許多個人用或 居家用的小型檢測儀器也就順應而生，例如血糖儀、尿酸 儀、血壓計及心電圖機等都屬此類。然而這些小型檢測儀 除了具有預防疾病發生之優點外，對病情的追蹤也特別具 3 1314648 =政盈，以血糖儀（glueGSesen而）為例，可用於隨時監控 ^病病患的血糖值，不但顯著提升醫師對病情的掌握^， :讓病患首次擁有自我疾病管控的空間。而這種自我：康 官理（self-health management)的模式除可降低醫護人員的 負擔’亦讓病患更關心也更瞭解自己的健康狀況，從而在 生活中根本地改善過去不當的習慣、飲食、作息甚至環境， 達=雙赢的局面。現今提倡的照護點監護（pGint_Gf謂二或 居家健康照護（in-home healthcare)也恰是基於這個觀點 推行的。 而，一次只檢驗一個參數或許對糖尿病患者而言是 足夠的，但許多錢並不如糖尿病般，只需檢測錢值就 足以評估病情，而是需要「同時」進行多種目標物的分析 才能較有效的瞭解與評估某器官的功能，因此只具有單一 功能之檢測儀實無法滿足市場之需求。由此可知，研發可 同％杈測夕項生理功能指標之小型檢測儀並應用於個人或 居家之健康照護體系’不僅市場潛力無窮亦是未來發展之 必然趨勢。 雖然現今可分析多種生理功能指標的小型檢測儀已有 許多商業化機型，但由國内自行研發的機種卻不多見，現 号有五加生技自行開發的必立康（Muitigure)可用於檢測 血糖及尿酸。然而其雖具有檢測兩種生理指標之功能，但 實際操作時仍是單次採血只能測定一種指標，無法進行單 一次採血即完成兩種測定。因此不僅使用上麻煩，也增加 受測者之困擾。 國外廠商方面，如羅式藥廠檢驗部門（R〇che 1314648As preventive medicine is popular around the world, prevention is more important than treatment, because early detection of early treatment is not only; to greatly enhance the chance of cure, it can also reduce the huge medical burden of subsequent derivation. Or the society has a very positive benefit. This is also the main reason why the implementation of Kang's inspection is highly valued and vigorously pursued in the advanced countries of the world. However, although large detectors can perform many physiological function tests very sensitively, they often fail to achieve the targets of small samples, rapid analysis and portable testing. Take metabolism-related diseases as an example. Although traditional automatic biochemical analyzers can routinely detect hydrocarbons, amino acids, vitamins or other small molecular weight compounds, they are bulky and expensive. It is only necessary to visit the medical institution in person to perform the test. In addition to the need for the nursing staff to take 5-1 "the blood, the analysis ^ can be completed by a more professional medical examiner, and the test report returns (n^r〇Und) -time) is also quite long, and it is completely unable to meet the requirements of the medical care end of the $-time detection or the general public's self-examination at any time. In response to the gradual rise of the individual's sense of self-health, many small-scale testing instruments used by individuals or households are also compliant. Health, such as blood glucose meters, uric acid meters, sphygmomanometers, and electrocardiographs, etc. are all of these. However, in addition to the advantages of preventing disease, these small detectors also track the disease. 3 1314648 = Zheng Ying, with blood sugar Instrument (glueGSesen), for example, can be used to monitor the blood sugar level of patients with disease at any time, not only significantly improve the doctor's grasp of the disease ^, : Let the patient have the space for self-control of disease for the first time. And this self: Kang Guanli (self -health management), in addition to reducing the burden on medical staff, also makes patients more concerned about and better understand their health, thus fundamentally changing their lives. Good past habits, diet, work and even the environment, up to a win-win situation. Today's advocated care point monitoring (pGint_Gf 2 or in-home healthcare is also based on this point of view. Testing only one parameter at a time may be enough for diabetics, but a lot of money is not like diabetes. Just checking the value of money is enough to assess the condition. Instead, you need to analyze the multiple targets at the same time to understand more effectively. And to evaluate the function of an organ, so the detector with only a single function can not meet the needs of the market. It can be seen that the development of a small detector with the physiological function index of the 杈 夕 并 并 并 并 并 并 并 并 并 并 并 并 并The system 'not only the market potential is infinite but also the inevitable trend of future development. Although there are many commercial models for small detectors that can analyze a variety of physiological function indicators today, there are few models developed by the domestic self-developed models. Muitigure, developed by Wujia Biotech, can be used to detect blood sugar and uric acid. The function of two kinds of physiological indicators is measured, but in actual operation, only one type of blood collection can only measure one index, and it is impossible to perform two kinds of determinations by single blood sampling, so it is not only troublesome to use, but also increases the trouble of the subject. Manufacturers, such as the Luoyi Pharmaceutical Inspection Department (R〇che 1314648
Diagnostics)所研發的三合一檢測儀（AccuTrend gct)，或聚 合物技術系統公司（Polymer Technology System)推出的多 功能檢測儀（BioScanner 2000)等都是多功能小型檢測儀的 代表。雖然這些檢測儀為手持式機型，攜帶非常方便，但 部都無法同時分析兩種以上的目標物，只能依檢測項目而 依序更換不同的試片，甚至連讀碼條也需更換。故每次採 血還是只能檢驗一個生理參數，相當的不便。 另外，目前已商業化之中型但仍屬可攜帶式的檢測 ,’如Abaxis研發的Picc〇1〇,重7·5公斤，雖可同時檢測 多種指標’但缺點是需血量大⑽μΐ-約5滴血)，分析時 間也長（15分鐘）。另外，亞培（編⑽)公司旗下μτατ所 推出的可攜式臨床檢測儀（P(mable CHnieal _丨Mr)，也有 需血量大的缺點（95队，約5滴血），而且更因為其採用電 化學原理，對樣品基質與環境參數的要求嚴格，故目前產 多集中於電解質與PH值的檢測，尚無法實用於— 指標。上述這些儀器具有大體積、高採血量或：法 個物檢測的缺點’正是大幅限制其應用及推廣於 豕照護市場的主因。由此可見，開發小型 採血遂行多目標物同時分析之檢測儀，不 在現度、也可提供更完整之生理功能評估， 4成長之健康照護市場中具有極高之競爭優勢。 【發明内容】 因此本發明的目 及其分析方法。 的就是在提供一種生化反應檢測卡匣 1314648 根據本發明之目的，提出一種使用上述生化反應檢測 卡匣之液體樣品的分析方法。使用上述生化反應檢測卡匣 =採樣裝置以對液體樣品進行定量採樣。然後由進氣口進 軋，以使儲存於稀釋液儲存槽之稀釋液體透過稀釋液入口 流至採樣裝置中，將採樣裝置内之液體樣品沖出至稀釋過 濾槽中。接著，由出氣口柚氣，使液體樣品通過稀釋過濾 槽之底部而流至液體槽内。繼續由出氣口抽氣，使液體樣 品沿著微流道流至末端，再流至反應槽中，以與位於反應 槽内之不同粉體試劑進行反應而分別形成不同的反應溶 液。最後，分析反應溶液之可見光-紫外光吸收強度，以測 定液體樣品中之數個待測物種之濃度。 依據本發明一較佳實施例，當反應槽之底部具有過濾 道與其相通’且微流道之末端具有過濾槽與其相通時，由 出氣口抽氣，使液體樣品沿著微流道流至末端，再流至過 濾槽與過濾槽内之試劑反應以形成預反應溶液。然後預反 應溶液再通過濾膜與過濾道流至相對應之反應槽中，以與 位於反應槽内之粉體試劑進行反應而形成反應溶液。 由上述可知，應用本發明較佳實施例可以對液體樣品 進行定量採樣、定量稀釋、過濾雜質以及同時分析數個物 種濃度之優點。 【實施方式] 本發明提供一種生化反應檢測卡匣及其分析方法，其 具有可定量採樣、定量稀釋、分離血球以及同時偵測金液 中多種成分之功能。 1314648 實施例一 請參照第^圖，其係㈣依照本發明—較佳實施例的 一種生化反應檢測卡匣的前方斜視爆炸圖。在第〗圖尹， 生化反應檢測卡e 由上至下可分解為滑板·、蓋板 3〇〇、採樣頭400、反應板500與底板6〇〇。 滑板2GG具有兩根短柱21〇,蓋板·上有兩個滑槽 31〇,而採樣頭400上則有兩個插孔41〇。因此，滑板2〇〇 兩根短柱210可分別透過蓋板_之兩個滑槽3 1 〇插入 採樣頭/〇上之兩個插孔41〇之中，讓使用者可透過滑板 200之月來回移動而使得採樣頭4〇〇也能前後來回移動。 雖然在此所舉之實施例的滑板2〇〇具有兩根短柱 210 ’蓋板300上有兩個滑槽31〇，且採樣頭4〇〇上有兩個 插孔410’但疋並不受以上之限制，所屬技術領域之人可以 ，其需求來修改之。例如，滑板2⑼只具有—根短柱21〇， 風板300上，、有一個滑槽31〇，且採樣頭4㈧上只有一個插 41 〇仍然元成上述之移動採樣頭4〇〇之功能。 、在採樣頭400之則端具有一個採樣開口 420，其形狀可 為各種形狀以容納各種不同之採樣裝置（未圖示於圖中）。請 亦同0夺參考第1B圖’其係繪示採樣頭柳之後方斜視圖。 在採樣頭400之後端具有一個稀釋液入口 43〇,其與採樣開 口 420相通’讓稀釋液體可由稀釋液入口 430流至採樣開 = 420紋量稀釋測試樣品。在稀釋液入口㈣之周圍呈 有-個環狀凹槽435’用以容納密封環。 在反應板500上，分佈有稀釋過濾槽5 1〇，用以容納採 1314648 樣頭400，讓採樣頭400可在其中前後移動以及提供稀釋測 試樣品之空間。稀釋過濾槽510前半段之底面上分佈有多 個過濾微孔520。在使用時，可在過濾孔520之上方或下方 貼附具有所需孔洞尺寸之濾膜（未圖示於圖中），用以濾掉測 試樣品中之固體物質，例如可過濾血液樣品中之紅血球與 各種白血球。上述濾膜例如可為使用纖維素（Cellulose)、聚 碳酸酯（polycarbonate)或尼龍（nylon)等材質所製成的濾 膜，例如有 Pall Corporation 的 BTS-SP-Asymmetric Membranes、SPECTRAL DIAGNOSTICS INC.的 Primecare separation membrane 或 GE Osmonics 的 PCTE membrane 等。 在稀釋過濾槽510之後端側壁上具有一個稀釋液出口 530。當採樣頭400置入於稀釋過濾槽510中並緊貼稀釋過 濾槽510之後端側壁時，稀釋液出口 530係與採樣頭400 之稀釋液入口 430相通。在進行稀釋樣品的步驟之前，稀 釋液出口 530係以薄膜或油密封起來。上述之薄膜例如可 為銘箱，而上述之油例如可為甘油（glycerol)。 在反應板500之稀釋過濾槽510的後方有一個稀釋液 儲存槽540，其前端與稀釋液出口 530相通。稀釋液儲存槽 540之另一側則有一條進氣道550與進氣口 560相通。在反 應板500之剩餘面積上分佈有數個反應槽570a、570b、 570c、570d，每個反應槽 570a、57〇b、570c、570d 各有一 條抽氣道580通往抽氣口 590。 在底板600上，分佈有液體槽610與微流道620，其中 微流道620具有數個末端A、B、C、D。當反應板500與 底板600重合時，每個末端A、B、C、D分別位於相對應 1314648 之每個反應槽570a、570b、570r、 WUc、570d之正下方並與其相 通。此外’為了加工方便’亦可將底板_上之微流道620 製做在反應板之背面，只纽意在輯時要使液體槽 61〇與微流道620能藉由一些接點相通即可。 承上所述，以血液樣品為例，當將滑板2〇〇、蓋板3⑼、 採樣頭400、反應板500與底板_組合起來之後，可利用 滑板200將採樣頭彻往前推出，讓採樣頭彻上所安裝The three-in-one detector (AccuTrend gct) developed by Diagnostics or the multi-function detector (BioScanner 2000) from Polymer Technology System is representative of the multi-function small detector. Although these detectors are hand-held models, they are very convenient to carry. However, it is impossible to analyze more than two types of objects at the same time. Only different test pieces can be replaced in sequence according to the test items, and even the code strips need to be replaced. Therefore, each blood collection can only test one physiological parameter, which is quite inconvenient. In addition, it is currently commercialized and still portable, but 'Picc〇1〇 developed by Abaxis, weighing 7.5 kg, can detect multiple indicators at the same time' but the disadvantage is that the blood volume is large (10) μΐ-about 5 drops of blood), the analysis time is also long (15 minutes). In addition, the portable clinical tester (P (mable CHnieal _丨Mr), which is launched by the company's μτατ, also has the disadvantage of requiring a large amount of blood (95 teams, about 5 drops of blood), and more because It adopts the principle of electrochemistry and has strict requirements on the sample matrix and environmental parameters. Therefore, the current production is mostly concentrated on the detection of electrolyte and PH value, which is not yet applicable to the indicators. These instruments have large volume, high blood collection or: The shortcomings of the object detection are the main reasons for greatly limiting its application and promotion in the market for sputum care. It can be seen that the development of a small blood collection sputum and multi-target simultaneous analysis of the detector, not in the present, can provide a more complete evaluation of physiological functions. 4, the growth of the health care market has a very high competitive advantage. [Summary of the Invention] Therefore, the object of the present invention and its analysis method is to provide a biochemical reaction detection cassette 1314648 according to the purpose of the present invention, Biochemical reaction detection method for the analysis of liquid samples of the cassette. The above biochemical reaction detection cassette = sampling device is used to determine the liquid sample. The sample is then sampled and rolled, so that the diluted liquid stored in the diluent storage tank flows through the diluent inlet to the sampling device, and the liquid sample in the sampling device is flushed out to the dilution filter tank. The air outlet mouth makes the liquid sample flow into the liquid tank through the bottom of the dilution filter tank. Continue to pump from the air outlet, so that the liquid sample flows along the micro flow channel to the end, and then flows into the reaction tank to be located Different powder reagents in the reaction tank are reacted to form different reaction solutions. Finally, the visible light-ultraviolet light absorption intensity of the reaction solution is analyzed to determine the concentration of several test species in the liquid sample. In a preferred embodiment, when the bottom of the reaction tank has a filter channel communicating therewith and the end of the microchannel has a filter tank communicating therewith, the gas is evacuated from the gas outlet, and the liquid sample flows along the microchannel to the end and then flows to the filter. The tank reacts with the reagent in the filter tank to form a pre-reaction solution, and then the pre-reaction solution flows through the membrane and the filter channel to the corresponding reaction tank to The powder reagent in the tank is reacted to form a reaction solution. From the above, it is known that the preferred embodiment of the present invention can quantitatively sample, quantitatively dilute, filter impurities and simultaneously analyze the concentration of several species. The invention provides a biochemical reaction detection cassette and an analysis method thereof, which have the functions of quantitative sampling, quantitative dilution, separation of blood cells and simultaneous detection of various components in the gold liquid. 1314648 For the first embodiment, please refer to the figure (4) A front squint explosion diagram of a biochemical reaction detecting cassette according to the present invention - a preferred embodiment. In the first diagram, the biochemical reaction detecting card e can be decomposed into a slide plate, a cover plate 3, and a sample from top to bottom. The head 400, the reaction plate 500 and the bottom plate 6. The slide plate 2GG has two short columns 21〇, the cover plate has two sliding grooves 31〇, and the sampling head 400 has two insertion holes 41〇. Therefore, the two short legs 210 of the sliding plate can be inserted into the two insertion holes 41 of the sampling head/〇 through the two sliding grooves 3 1 of the cover plate respectively, so that the user can pass through the sliding plate 200 Moving back and forth allows the sampling head 4 to move back and forth. Although the slider 2 of the embodiment herein has two short posts 210', the cover 300 has two chutes 31〇, and the sampling head 4 has two jacks 410' but it is not Subject to the above limitations, those skilled in the art can modify the needs thereof. For example, the slider 2 (9) has only a short stub 21 〇, the wind panel 300 has a chute 31 〇, and only one plug 41 采样 on the sampling head 4 (eight) is still functioning as the above-described moving sampling head 4 。. At the end of the sampling head 400, there is a sampling opening 420 which is shaped to accommodate a variety of different sampling devices (not shown). Please also refer to Figure 1B for the same drawing. At the rear end of the sampling head 400 is a diluent inlet 43〇 that communicates with the sampling opening 420. The dilution liquid can be flowed from the diluent inlet 430 to the sampled open = 420 grain dilution test sample. An annular groove 435' is formed around the diluent inlet (4) for receiving the seal ring. On the reaction plate 500, a dilution filter tank 51 is disposed to accommodate the sample 1314648, so that the sampling head 400 can move back and forth therein and provide a space for diluting the test sample. A plurality of filtering micropores 520 are distributed on the bottom surface of the first half of the dilution filter tank 510. In use, a filter having a desired pore size (not shown) may be attached above or below the filter well 520 to filter out solid matter in the test sample, such as in a filterable blood sample. Red blood cells and various white blood cells. The filter membrane may be, for example, a membrane made of a material such as Cellulose, polycarbonate or nylon, such as BTS-SP-Asymmetric Membranes and SPECTRAL DIAGNOSTICS INC. of Pall Corporation. Primecare separation membrane or GE Osmonics' PCTE membrane. There is a diluent outlet 530 on the side wall of the end of the dilution filter tank 510. The diluent outlet 530 is in communication with the diluent inlet 430 of the sampling head 400 when the sampling head 400 is placed in the dilution filter tank 510 and abuts against the rear side wall of the dilution filter tank 510. The diluent outlet 530 is sealed with a film or oil prior to the step of diluting the sample. The above film may be, for example, a name box, and the above oil may be, for example, glycerol. A diluent storage tank 540 is disposed behind the dilution filter tank 510 of the reaction plate 500, and its front end communicates with the diluent outlet 530. On the other side of the diluent storage tank 540, an inlet 550 communicates with the inlet 560. A plurality of reaction tanks 570a, 570b, 570c, 570d are distributed over the remaining area of the reaction plate 500, and each of the reaction tanks 570a, 57〇b, 570c, 570d has an air suction passage 580 leading to the air suction port 590. On the bottom plate 600, a liquid tank 610 and a micro flow path 620 are distributed, wherein the micro flow path 620 has a plurality of ends A, B, C, D. When the reaction plate 500 is overlapped with the bottom plate 600, each of the ends A, B, C, and D is located immediately below and in communication with each of the reaction tanks 570a, 570b, 570r, WUc, 570d of the corresponding 1314648. In addition, 'for the convenience of processing', the micro-channel 620 on the bottom plate can be made on the back side of the reaction plate, and the liquid channel 61 〇 and the micro-channel 620 can be connected by some contacts. can. As described above, taking the blood sample as an example, after the slide plate 2, the cover plate 3 (9), the sampling head 400, the reaction plate 500 and the bottom plate _ are combined, the sampling head can be pushed forward through the slide plate 200 to allow sampling. Head mounted
之採樣裝置來進行血液採樣。接著，將滑板期向後移動， 1採樣頭400抵住稀釋過渡槽51〇之後方側壁。此時，採 樣碩400之稀釋液入口 43〇與稀釋過濾槽51〇之稀釋液出 口 530密接在一起。 然後使用幫浦（未圖示於圖中）從反應板進氣口 56〇灌 入氣體，透過進氣道550增加稀釋液儲存槽54〇之壓力， 擠壓稀釋液儲存槽54G中所儲存之稀釋液體，讓其自稀釋 液出口 530與稀釋液入口 43〇流入採樣頭4〇〇之中，將採 樣装置上之血液樣品沖入稀釋過濾槽51〇之中進行定量稀 釋。接著，稀釋之血漿會通過過濾微孔52〇進入液體槽6忉 之中，而血液中之各種血球與其他固體則被留在稀釋過濾 槽5 1 〇之中。 “ 再來，使用幫浦（未圖示於圖中）自反應板5〇〇之各個抽 Μ 苹口 590抽氣，讓稀釋血漿可自液體槽6丨〇沿著微流道62〇 到達微流道620之各個末端A、B、c、D ,再通入位於各 末*而A、B、C、D上之各個反應槽570a、570b、570c與570d 甲’讓血漿可以與置於反應槽570a、570b、570c與57〇d 之各種粉體試劑、將試劑固定在膠體内之膠體試劑或將 1314648 試劑固定在玻璃球或磁性粒子上之固體試劑進行反應。由 於多數生化檢測都會加入各種不同之呈色試劑，因此可以 使用一般之可見光-紫外光光源與偵測器分置反應槽 570a、570b、570c與570d之上下兩端，以直接對反應槽 570a、570b、570c與570d内反應後之反應溶液進行可見光 -紫外光之吸收強度測定’再據此來推算欲分析之物種的濃 度。 由於是利用可見光-紫外光之吸收強度來直接測定位 於反應槽570a、570b、570c與570d内之欲分析物種，所 以反應板500之顏色較佳為黑色，以避免外界光線及相鄰 反應槽的干擾可見光-紫外光之吸收強度的測定。而蓋板 300與底板600之顏色則較佳為透明無色，以提升可見光_ 紫外光之吸收強度的測定靈敏度。 此外，反應槽57〇a、57〇b、57〇c與57〇d之深摩與直 徑以及樣品的稀釋倍數與可見光-紫外光之吸收強度的檢 測靈敏度有一定的關係。反應槽57〇a、57〇b、57〇c與57〇d 之深度一般為〇_5 - 1〇 mm，而其直徑一般為〇 5 _ 1〇讯茁。 若欲分析物種之濃度較低，例如為表一所列之物種， 則反應槽570a、570b、570c與570d之深度較佳為2 —8mm, 更佳為4 - 6 mm。反應槽57〇a、57〇b、57〇c與57〇d之直 徑較佳為2-5mm，更佳為2_3mm。而樣品之稀釋倍數 一般為1- 20倍，較佳為5_15倍，更佳為8— 12倍。 表一：低濃度待測物種 11 1314648 生化分子 項目 濃度（mg/dL) 尿酸(Uric acid) 3.5 - 7.2 膽紅素（Bilirubin) 0.2 - 1 金中尿素氮（Blood urea nitrogen) 7-18 肌酸針（Creatinine) 0.6 - 1.2 酵素 項目 濃度 脂肪水解酵素（lipase) 0 - 1 (U/mL) 酸性填酸酵素（Acid Phosphatase; ACP) 2.5 - 11.7 (U/L) 電解質 項目 濃度(mEq/L) 鉀離子（Potassium) 3.4-5 若欲分析物種之濃度較高，例如為表二所列之物種， 則反應槽570a、570b、570c與570d之深度較佳為0.5 - 5 mm，更佳為 0.8 - 1.2 mm。反應槽 570a、5 70b、570c 與 570d 之直徑較佳為2 - 5 mm，更佳為2 - 3 mm。而樣品之稀釋 倍數一般為10 - 100倍，較佳為10 - 50倍，更佳為25 - 35 倍。 12 1314648 表二：高濃度待測物種 生化分子 項目 - __ 濃度（mg/dL) 葡萄糖(Glucose) 70 - 110 二酸甘油 S旨（Triglyceride) —— 67-157 高密度脂蛋白膽固醇(HDL-c) 29-60 總膽固醇（Total cholesterol) 140-200 酵素 項目 濃度(U/L) 乳酸去氫酶（Lactate dehydrogenase) 100 - 225 丙胺酸轉胺酶（Alanine aminotransferase) 6-37 鹼性磷酸酵素（Alkaline phosphatase) 30-90 天門冬胺酸轉胺酶(Aspartate aminotransferase) 5-30 殿粉水解酵素（Amylase) 60-180 肌酸酐酶（Creatine kinase) 15 - 160 轉 Τ -麩胺酶（7 -glutamyl transferase) 6-45 電解質 項目 濃度（mEq/L) 13 1314648 氯離子（Chloride) 98 - 106 總二氧化碳（Total C02) 22 - 29 鈉離子（Sodium) 135-145 實施例二 請參照第2圖，其係繪示依照本發明另一較佳實施例 的一種生化反應檢測卡匣的前方斜視爆炸圖。在第3圖中， 生化反應檢測卡匣110由上至下可分解為滑板200、蓋板 300、採樣頭400、反應板500與底板700。 在此實施例中，滑板200、蓋板300、採樣頭400與反 應板500之結構與實施例一相同，只有底板700的部分有 更改設計。在底板700上，亦分佈有液體槽710與微流道 720，其中微流道620亦具有數個末端A、B、C、D。當反 應板500與底板700重合時，每個末端A、B、C、D分別 位於相對應之每個反應槽570a、570b、570c、570d之正下 方並與其相通。 但是在底板700的微流道720a、720b、720c、720d之 設計上，係採分離式設計，亦即數條微流道720a、720b、 720c、720d分別從液體槽710延伸出來，不需經過分支就 分別直接通往端點A、B、C、D。而底板600的微流道620 設計則採集中式設計，亦即只有一條微流道620自液體槽 610延伸出來，然後再經過分支才分別通往端點A、B、C、 D。當然，為了加工方便，也可以將底板700之微流道720 製作於反應板500的底部，只要注意在設計時要使液體槽 14 1314648 710與微流道720a、720b、720c、720d能藉由—些接點相 通即可。 使用生化反應檢測卡匣110來檢測樣品的程序與生化 反應檢測卡匣100相同，因此不再贅述之。 實施例三 請參照第3圖，其係繪示依照本發明又一較佳實施例 的一種生化反應檢測卡匣的前方斜視爆炸圖。在第3圖中， 生化反應檢測卡匣120由上至下可分解為滑板2〇〇、蓋板 300、採樣頭400、反應板500與底板800。 在此實施例中，滑板200 '蓋板300與採樣頭4〇〇之結 構與實施例一相同，只有反應板500與底板8〇〇的部分有 更改設計。 在反應板500的部分，在反應槽570a底部之一側延伸 出各·過慮道575。在底板800的部分，大致與底板7〇〇 相同。不同的部分有微流道820a的長度比微流道72〇a的 長度短’而且在微流道820a的末端還設置了一個過濾槽 830。當反應板500與底板8〇〇上下重合時，底板8〇〇之過 濾、槽830位在反應板500之過遽道575之正下方，且彼此 相通，但是在過濾槽830與過濾道575之間要夾一層濾膜 840。濾膜840例如可為使用纖維素（Cellul〇se)、聚碳酸酯 (polycarbonate)或尼龍（nylon)等材質所製成的濾膜，其孔徑 可為 0.2 - 20 μιη，較佳為 〇.2 一 3 μηι。 因此在使用生化反應檢測卡匣丨2〇時，可在過濾槽83〇 之内置放沈澱試劑，以進一步去除測試樣品中之一些干擾 15 1314648 物質。例如，要測定血液中之高密度脂肪（HDL)的含量時， 血漿中之許多其他類型脂蛋白會干擾高密度脂蛋白膽固醇 的測定結果。因此可在過遽槽830内置放dextran sulfate 及氯化鎂（MgCl2)，當血漿自液體槽810通過微流道820a 至過濾槽830時，血漿中之干擾物會和上述試劑作用而形 成膠體。在血漿通過過濾槽830與過濾道575之間的濾膜 840之後，就可讓去除了干擾物之血漿繼續流至反應槽 5 70a，進行後續之反應與檢測步驟。 安裝在採樣頭上之各種採樣裝置 在生化反應檢測卡匣100、110、120之採樣頭400之 採樣開口 420中可安置各種採樣裝置，茲介紹如下。 請同時參照第4A-4B圖，其係繪示虹吸管之採樣裝置 安裝在採樣頭上之示意圖。在圖中，虹吸管440與採樣頭 400為一體成形，在虹吸管440之内部有一中空通道445 與採樣頭400後方之稀釋液入口 430相通。 因此在取樣時，虹吸管440係直接利用虹吸現象吸取 液體樣品。然後在後續由進氣口 560打氣時，稀釋液會由 反應板500上之稀釋液儲存槽540通過稀釋液出口 530與 稀釋液入口 430，注入至中空通道445將液體樣品沖出至稀 釋過濾槽510内，進行後續的分析步驟。 請參照第5A-5B圖，其係繪示採樣環之採樣裝置安裝 在採樣頭上之示意圖。在圖中，採樣環450係由採樣頭400 前方之採樣開口 420插入，在採樣環450之把柄452與固 定座45 4之中間亦有一中空通道45 6。中空通道456之前方 16 1314648 中空通道456之後方與稀 與採樣環450之内部空間相通 釋液入口 430相通。 =採樣時，只要直接讓採樣環价接觸液體樣品，則 以義液體樣品之表面張力讓其附著在採樣環45〇上。因 此當稀釋液自稀釋液入口 43〇流至中空通道伙内，再流 ^採樣環450之内部空間時，就可以將附著在採樣環45〇 之液體樣品直接沖人至反應板5⑽上之稀釋過渡槽51〇 内，進行後續之分析步驟。The sampling device performs blood sampling. Next, the sliding period is moved backward, and the sampling head 400 is pressed against the side wall of the rear side of the dilution transition groove 51. At this time, the diluent inlet 43 of the sample 400 is closely adhered to the diluent outlet 530 of the dilution filter tank 51. Then, a pump (not shown) is used to inject gas from the reaction port inlet 56, and the inlet 550 is used to increase the pressure of the diluent storage tank 54, and is squeezed into the diluent storage tank 54G. The liquid is diluted, and is allowed to flow from the diluent outlet 530 and the diluent inlet 43 into the sampling head 4, and the blood sample on the sampling device is flushed into the dilution filter tank 51 to be quantitatively diluted. Next, the diluted plasma enters the liquid tank 6 through the filtration micropores 52, and various blood cells and other solids in the blood are left in the dilution filter tank 51. “Further, use the pump (not shown in the figure) to pump the gas from each of the reaction plates 5, so that the diluted plasma can be transferred from the liquid tank 6丨〇 along the microchannel 62〇. Each of the ends A, B, c, and D of the flow path 620 is re-introduced into each of the reaction tanks 570a, 570b, 570c, and 570d at each of the ends* and A, B, C, and D. The various powder reagents of the tanks 570a, 570b, 570c and 57〇d, the colloidal reagents for fixing the reagents in the gel or the solid reagents for immobilizing the 1314648 reagent on the glass spheres or magnetic particles are reacted. Different coloring reagents, so that the upper and lower ends of the reaction tanks 570a, 570b, 570c and 570d can be separated by a general visible light-ultraviolet light source and a detector to directly react to the reaction tanks 570a, 570b, 570c and 570d. The subsequent reaction solution is subjected to the measurement of the absorption intensity of visible light-ultraviolet light. The concentration of the species to be analyzed is then estimated. The absorption intensity of visible light-ultraviolet light is used to directly determine the concentration in the reaction tanks 570a, 570b, 570c and 570d. Desire analysis Species, so the color of the reaction plate 500 is preferably black to avoid the interference of visible light and ultraviolet light by external light and adjacent reaction grooves. The color of the cover plate 300 and the bottom plate 600 is preferably transparent and colorless. To improve the sensitivity of the absorption of visible light _ ultraviolet light. In addition, the deep friction and diameter of the reaction tanks 57〇a, 57〇b, 57〇c and 57〇d and the dilution factor of the sample and the absorption intensity of visible light-ultraviolet light The detection sensitivity has a certain relationship. The depths of the reaction tanks 57〇a, 57〇b, 57〇c and 57〇d are generally 〇5 - 1〇mm, and the diameter is generally 〇5 _ 1〇. If the concentration of the species to be analyzed is low, such as the species listed in Table 1, the depth of the reaction tanks 570a, 570b, 570c and 570d is preferably 2-8 mm, more preferably 4-6 mm. Reaction tank 57〇a The diameter of 57〇b, 57〇c and 57〇d is preferably 2-5 mm, more preferably 2_3 mm, and the dilution factor of the sample is generally 1-20 times, preferably 5-15 times, more preferably 8-12. Table 1: Low concentration of tested species 11 1314648 Biochemical molecular project concentration (mg/dL) Uric acid 3 .5 - 7.2 Bilirubin 0.2 - 1 Blood urea nitrogen 7-18 Creatinine 0.6 - 1.2 Enzyme Item Concentration Lipase 0 - 1 (U/mL Acid Phosphatase (ACP) 2.5 - 11.7 (U/L) Electrolyte concentration (mEq/L) Potassium (3.4) If the concentration of the species to be analyzed is high, for example, listed in Table 2. For the species, the depth of the reaction tanks 570a, 570b, 570c and 570d is preferably from 0.5 to 5 mm, more preferably from 0.8 to 1.2 mm. The diameter of the reaction tanks 570a, 5 70b, 570c and 570d is preferably 2 - 5 mm, more preferably 2 - 3 mm. The dilution ratio of the sample is generally 10 - 100 times, preferably 10 - 50 times, more preferably 25 - 35 times. 12 1314648 Table 2: Biochemical Molecular Projects of High Concentration Species to be Tested - __ Concentration (mg/dL) Glucose 70 - 110 Diglyceride S (Triglyceride) - 67-157 High Density Lipoprotein Cholesterol (HDL-c 29-60 Total cholesterol 140-200 Enzyme Item Concentration (U/L) Lactate dehydrogenase 100 - 225 Alanine aminotransferase 6-37 Alkaline Phosphate (Alkaline) Phosphatase) 30-90 Aspartate aminotransferase 5-30 Amylase 60-180 Creatinase kinase 15 - 160 Transferring - 7-glutamyl transferase 6-45 Electrolyte concentration (mEq/L) 13 1314648 Chloride (Chloride) 98 - 106 Total carbon dioxide (Total C02) 22 - 29 Sodium (135) 135-145 For the second embodiment, please refer to Figure 2, A front oblique exploded view of a biochemical reaction detecting cassette according to another preferred embodiment of the present invention is shown. In Fig. 3, the biochemical reaction detecting cassette 110 is decomposed into a slide plate 200, a cap plate 300, a sampling head 400, a reaction plate 500, and a bottom plate 700 from top to bottom. In this embodiment, the structure of the slider 200, the cover 300, the sampling head 400, and the reaction plate 500 is the same as that of the first embodiment, and only a portion of the bottom plate 700 has a modified design. On the bottom plate 700, a liquid tank 710 and a micro flow path 720 are also distributed, wherein the micro flow path 620 also has a plurality of ends A, B, C, and D. When the reaction plate 500 is overlapped with the bottom plate 700, each of the ends A, B, C, and D is located immediately below and in communication with each of the corresponding reaction tanks 570a, 570b, 570c, 570d. However, in the design of the micro flow passages 720a, 720b, 720c, and 720d of the bottom plate 700, a separate design is adopted, that is, a plurality of micro flow passages 720a, 720b, 720c, and 720d respectively extend from the liquid tank 710 without passing through The branches go directly to endpoints A, B, C, and D, respectively. The micro flow channel 620 of the bottom plate 600 is designed to capture the Chinese design, that is, only one micro flow channel 620 extends from the liquid channel 610 and then branches to the terminals A, B, C, and D, respectively. Of course, for the convenience of processing, the micro flow channel 720 of the bottom plate 700 can also be formed on the bottom of the reaction plate 500, as long as the liquid trough 14 1314648 710 and the micro flow channels 720a, 720b, 720c, 720d can be designed by the design. - Some contacts can be connected. The procedure for detecting a sample using the biochemical reaction detection cassette 110 is the same as that of the biochemical reaction detection cassette 100, and therefore will not be described again. Embodiment 3 Referring to Figure 3, there is shown a front oblique exploded view of a biochemical reaction detecting cassette in accordance with still another preferred embodiment of the present invention. In Fig. 3, the biochemical reaction detecting cassette 120 is decomposed into a slide plate 2, a cover plate 300, a sampling head 400, a reaction plate 500, and a bottom plate 800 from top to bottom. In this embodiment, the structure of the slider 200' cover 300 and the sampling head 4 is the same as that of the first embodiment, and only the portion of the reaction plate 500 and the bottom plate 8 has a modified design. In the portion of the reaction plate 500, each of the bypass passages 575 is extended on one side of the bottom of the reaction vessel 570a. The portion of the bottom plate 800 is substantially the same as the bottom plate 7A. The different portions have the length of the microchannel 820a shorter than the length of the microchannel 72〇a' and a filter tank 830 is provided at the end of the microchannel 820a. When the reaction plate 500 and the bottom plate 8 are vertically overlapped, the bottom plate 8 is filtered, and the groove 830 is located directly below the bypass 575 of the reaction plate 500, and communicates with each other, but in the filter tank 830 and the filter channel 575 A filter 840 is sandwiched between them. The filter membrane 840 may be, for example, a membrane made of a material such as cellulose (cellulose), polycarbonate (carbonic acid) or nylon (nylon), and has a pore diameter of 0.2 to 20 μm, preferably 〇.2. One 3 μηι. Therefore, when the biochemical reaction detection cassette 2 is used, a precipitation reagent can be placed in the filter tank 83 to further remove some of the interference 15 1314648 substances in the test sample. For example, when measuring the high-density fat (HDL) content in blood, many other types of lipoproteins in plasma interfere with the determination of high-density lipoprotein cholesterol. Therefore, dextran sulfate and magnesium chloride (MgCl2) can be placed in the over-tank 830. When the plasma passes through the microchannel 820a to the filter tank 830 from the liquid tank 810, the interference substance in the plasma acts on the reagent to form a colloid. After the plasma passes through the filter 840 between the filter tank 830 and the filter channel 575, the plasma from which the interferent has been removed is allowed to continue to the reaction tank 5 70a for subsequent reaction and detection steps. Various sampling devices mounted on the sampling head Various sampling devices can be placed in the sampling opening 420 of the sampling head 400 of the biochemical reaction detecting cassettes 100, 110, 120, which are described below. Please also refer to Fig. 4A-4B, which is a schematic diagram showing the installation of the siphon sampling device on the sampling head. In the figure, the siphon tube 440 is integrally formed with the sampling head 400. Inside the siphon tube 440, a hollow passage 445 communicates with the diluent inlet 430 behind the sampling head 400. Therefore, at the time of sampling, the siphon tube 440 directly draws a liquid sample by siphoning. Then, when subsequently inflated by the air inlet 560, the diluent is passed from the diluent storage tank 540 on the reaction plate 500 through the diluent outlet 530 and the diluent inlet 430, and injected into the hollow passage 445 to flush the liquid sample to the dilution filter tank. In 510, a subsequent analysis step is performed. Please refer to FIG. 5A-5B, which is a schematic diagram showing the sampling device of the sampling ring mounted on the sampling head. In the figure, the sampling loop 450 is inserted by the sampling opening 420 in front of the sampling head 400. There is also a hollow passage 45 6 between the handle 452 of the sampling ring 450 and the fixing seat 45 4 . The hollow channel 456 is in front of the 16 1314648 hollow channel 456 and communicates with the internal space of the rare sample ring 450 to communicate with the liquid delivery inlet 430. = When sampling, as long as the sampling loop is directly exposed to the liquid sample, the surface tension of the liquid sample is allowed to adhere to the sampling ring 45〇. Therefore, when the diluent flows from the diluent inlet 43 into the hollow channel and flows into the inner space of the sampling ring 450, the liquid sample attached to the sampling ring 45〇 can be directly washed onto the reaction plate 5 (10). In the transition tank 51, a subsequent analysis step is performed.
^參照第6A-6B圖，其係綠示刷毛之採樣裝置安裝在 ^頭上之示意圖。在圖中，一束刷毛彻係直接插入至 讀頭400之採樣開口 之内，此束刷毛偏之中央處 父佳為有一中空通道（未圖示於圖中）。因此，刷毛460之間 的微細空間鱼中办、苦θ 一 二、、疋與知樣開口 420與稀釋液入口 430直接相通的。 在才木樣時，刷毛460 {利用虹吸現象將液體樣品吸入。 在稀釋樣品時，稀釋液龍稀釋液人口 430流至採樣開口 420,將刷毛之間所吸附之液體樣品沖刷至稀釋過渡槽训 内，以進行後續的分析步驟。 <月“、帛7Α·7Β目’其係1會示虹吸片之採樣裝置安裝 在知樣頭上之不思圖。在圖中，將採樣頭·之採樣開口 420自前端中央處移至前端底緣處，且將採樣開π 420之高 度降低虹吸片470則貼緊在採樣頭400之底部，與採樣 頭400共同形成採樣開口 42〇。 、：採认開口 420之高度很小，因此還是利用虹吸現 象將液體樣叩吸入至採樣開口 42〇之内。由於採樣開口㈣ 17 1314648 接:、稀釋液入口 430直接相通，所以在稀釋液體樣品時， 釋液亦從稀釋液人σ 43G流至採樣開口細，將採樣開口 420内所吸附之液體樣品沖刷至稀釋過遽槽51G内，以進行 後續的分析步驟。 ，請參照第8A_8B圖，其係繪示薄膜之採樣裝置安裝在 知樣頭上之示意圖。基本上，薄膜之採樣裝置與虹吸片之 采樣竑置疋樣的，唯一不同的是在虹吸片47〇上之採樣 開口 420中夾入採樣薄膜48〇來吸收液體樣品。 因此在採樣時，只要將採樣薄膜48〇伸入至液體樣品 中，則採樣薄膜480可自然吸收液體樣品。又，由於採樣 開420係與稀釋液入口 430直接相通，所以在稀釋液體 樣品時，稀釋液亦從稀釋液入口 430流至採樣開口 42〇，將 採樣薄膜480所吸附之液體樣品沖刷至稀釋過濾槽51〇 内’以進行後續的分析步驟。 月參照第9 A -9 B圖，其係繪示微流道之採樣裝置安裝 在採樣頭上之示意圖。基本上，微流道之採樣裝置與虹吸 片之採樣裝置是一樣的，唯一不同的是將原先之採樣開口 420的連續空間之前段部分劃分成多條平行的微流道49〇， 而採樣開口 420的後段部分仍保留為一連續空間之缓衝區 495。 因此取樣時，微流道490是利用虹吸現象將液體樣品 及入Λ、：後在稀釋液體樣品時，稀釋液從稀釋液入口 430 流至緩衝區495’再將微流道490所吸附之液體樣品沖刷至 稀釋過濾槽5 1 〇内，以進行後續的分析步驟。 18 !314648^ Referring to Figures 6A-6B, which is a schematic view of the green bristles sampling device mounted on the head. In the figure, a bundle of bristles is inserted directly into the sampling opening of the read head 400, and the center of the bundle of bristles has a hollow passage (not shown). Therefore, the fine space between the bristles 460 is directly connected to the diluent inlet 420. When the wood is sampled, the bristles 460 {use the siphon phenomenon to inhale the liquid sample. When the sample is diluted, the dilution dragon dilution population 430 flows to the sampling opening 420, and the liquid sample adsorbed between the bristles is flushed into the dilution transition slot for subsequent analysis steps. <Monthly, 帛7Α·7Β目', the system 1 will show that the sampling device of the siphon is mounted on the sample head. In the figure, the sampling opening 420 of the sampling head is moved from the front center to the front end. At the bottom edge, and the height of the sampling opening π 420 is reduced, the siphon sheet 470 is closely attached to the bottom of the sampling head 400, and forms a sampling opening 42 with the sampling head 400. The height of the recognition opening 420 is small, so The liquid sample is sucked into the sampling opening 42 by the siphon phenomenon. Since the sampling opening (4) 17 1314648 is connected, the diluent inlet 430 is directly connected, so when the liquid sample is diluted, the liquid is also discharged from the diluent human σ 43G to The sampling opening is thin, and the liquid sample adsorbed in the sampling opening 420 is flushed into the dilution through-slot 51G for subsequent analysis steps. Please refer to FIG. 8A_8B, which shows that the sampling device of the film is mounted on the sample head. Basically, the sampling device of the film is sampled with the sampling device of the siphon, the only difference is that the sampling film 48 is sandwiched in the sampling opening 420 on the siphon 47〇 to absorb the liquid sample. Therefore, at the time of sampling, as long as the sampling film 48〇 is inserted into the liquid sample, the sampling film 480 can naturally absorb the liquid sample. Further, since the sampling 420 system is in direct communication with the diluent inlet 430, when the liquid sample is diluted, The diluent also flows from the diluent inlet 430 to the sampling opening 42A, and the liquid sample adsorbed by the sampling film 480 is flushed into the dilution filter tank 51' for subsequent analysis steps. Referring to Figure 9A-9B, It is a schematic diagram showing the sampling device of the micro flow channel mounted on the sampling head. Basically, the sampling device of the micro flow channel is the same as the sampling device of the siphon film, the only difference is that the original sampling opening 420 is in front of the continuous space. The segment portion is divided into a plurality of parallel microchannels 49〇, and the rear portion of the sampling opening 420 remains as a continuous space buffer 495. Therefore, during sampling, the microchannel 490 utilizes a siphon phenomenon to introduce a liquid sample into the space. Λ,: After diluting the liquid sample, the diluent flows from the diluent inlet 430 to the buffer zone 495', and then the liquid sample adsorbed by the microchannel 490 is flushed to the diluted sample. The filter tank 5 1 is inside the chamber for subsequent analysis steps. 18 !314648
由上述本發明較佳實施例可知，上述所揭露之生化反 應，，卡匿可搭配各種採樣裝置之不同固定容量，而具有 可疋里採樣之功能。在採樣完成後，依據反應板上之稀釋 液儲存槽所儲存之定量稀釋液，還能定量稀釋樣品。後面 只要利用&氣與進氣步驟之搭g己，則可同時進行樣品中多 種=測物種之—連串過濾、、混合、反應以及測定待測物種 之派度的步驟，因此使用上十分簡便快速。此外，生化反 應檢測卡£之設計製造上亦十分簡易，可利用適合的塑膠 材料[例如聚曱基丙烯酸甲西旨（Poly methyl methacrylate; PMMA)、聚苯乙烯（Polystyrene; PS)或聚碳酸酯 (polycarbonate; PC)] ’經由簡單加工即可完成因此十分適 宜量產成拋棄式生化反應檢測卡匣’以供實驗室或檢測單 位之每日大量檢測之用。 雖本發明已以較佳實施例揭露如上，然其並非用以 限定本發明’任何熟習此技藝者，在不脫離本發明之精神 和範圍内，當可作各種之更動與潤飾，因此本發明之保護 範圍當視後附之申請專利範圍所界定者為準。 【圖式簡單說明】 為讓本發明之上述和其他目的、特徵、優點與實施例 能更明顯易懂，所附圖式之詳細說明如下： 第1A圖是繪示依照本發明一較佳實施例的一種生化 反應檢測卡匣的前方斜視爆炸圖。 第1B圖係繪示採樣頭4〇〇之後方斜視圖。 第2圖係繪示依照本發明另一較佳實施例的一種生化 19 1314648 反應檢測卡e的前方斜視爆炸圖。 第3圖係繪示依照本發明又 反應檢測卡匣的前方 又4實施例的一種生化 J則万斜視爆炸圖。 樣裳置安裝在採樣頭 上 第4A-4B圖係綠示虹吸管之採 之示意圖。 置安裝在採樣頭上 第5A_5B圖係緣示採樣環之採樣裝 之示意圖。 〜 第6A-6B圖係纷 示意圖 示刷毛之採樣裝置安裝在採樣頭上之 之二7B圖係綠示虹吸片之採樣裝置安裝在採樣頭上 示意ί Μ·8Β圖係输示薄膜之採樣裝置安裝在採樣頭上之 第9 A 9 Β圖係綠示微流道 上之示意圖。 、之知樣裝置安裝在採樣頭 【主要元件符號說明】 1〇〇 110、12〇 ·生化反應檢測卡匣200:滑板 210 :短柱 300 :蓋板 310 :滑槽 400:採樣頭 410 ·•插孔 420 ·採樣開口 :稀釋液入口 435 . %狀凹样 440 :虫X吸營 m 445、456:中空 450 :採樣淨 二遇道 衣 452:把柄 20 1314648 454 :固定座 470 :虹吸片 490 :微流道 500 :反應板 520 :過濾微孔 540 :稀釋液儲存槽 560 :進氣口 4 6 0 ·刷毛i 480 :採樣薄膜 495 :缓衝區 510 :稀釋過濾槽 530 :稀釋液出口 550 :進氣道 575 :過濾道 570a、570b、570c、570d :反應槽 580 :抽氣道 590 :抽氣口 600、700、800 ··底板 610、710、810 :液體槽 620 :微流道 720a、720b、720c、720d :微流道 830 :過濾槽 820a、820b、820c、820d :微流道 A、B、C、D :末端 840 :濾膜It can be seen from the above preferred embodiment of the present invention that the biochemical reaction disclosed above can be combined with different fixed capacities of various sampling devices, and has the function of sampling. After the sampling is completed, the sample can also be diluted quantitatively based on the quantitative dilution stored in the diluent reservoir on the reaction plate. As long as you use the & gas and air intake steps, you can simultaneously perform multiple steps in the sample = series of filters, mixing, reaction, and determination of the degree of the species to be tested. Simple and fast. In addition, the biochemical reaction test card is also very simple to design and manufacture, and can use suitable plastic materials [such as polymethyl methacrylate (PMMA), polystyrene (PS) or polycarbonate (Polymethyl methacrylate; PMMA). Polycarbonate; PC)] 'After simple processing, it is very suitable for mass production of disposable biochemical reaction detection cassettes' for daily testing of laboratories or testing units. The present invention has been disclosed in the above preferred embodiments, and is not intended to limit the invention, and the invention may be modified and modified without departing from the spirit and scope of the invention. The scope of protection is subject to the definition of the scope of the patent application. BRIEF DESCRIPTION OF THE DRAWINGS The above and other objects, features, advantages and embodiments of the present invention will become more <RTIgt; An example of a biochemical reaction detects the front squint exploded view of the cassette. Fig. 1B is a perspective view showing the sampling head 4〇〇. 2 is a front oblique exploded view of a biochemical 19 1314648 reaction detecting card e according to another preferred embodiment of the present invention. Fig. 3 is a view showing a biochemical J squint exploded view of the fourth embodiment of the reaction detecting cassette in accordance with the present invention. The sample is installed on the sampling head. The 4A-4B is a schematic diagram of the green siphon. Installed on the sampling head. Figure 5A_5B shows the sampling device of the sampling loop. ~ The 6A-6B diagram shows the sampling device of the bristles installed on the sampling head. The 7B system of the green siphon is installed on the sampling head. The sampling device is installed on the sampling film. The 9th 9th image on the sampling head is a schematic diagram of the green microchannel. The known device is installed in the sampling head. [Main component symbol description] 1〇〇110, 12〇·Biochemical reaction detection card 200: Skateboard 210: Short column 300: Cover plate 310: Chute 400: Sampling head 410 • Jack 420 · Sampling opening: Diluent inlet 435. % concave 440: Insect X suction camp m 445, 456: Hollow 450: Sampling net two lanes 452: Handle 20 1314648 454: Mounting seat 470: Siphon sheet 490 : micro flow channel 500 : reaction plate 520 : filtration micro hole 540 : diluent storage tank 560 : air inlet 4 6 0 · bristles i 480 : sampling film 495 : buffer zone 510 : dilution filter tank 530 : diluent outlet 550 : Inlet 575: Filter passages 570a, 570b, 570c, 570d: Reaction tank 580: Exhaust passage 590: Exhaust ports 600, 700, 800 · Floor plates 610, 710, 810: Liquid tank 620: Microchannels 720a, 720b , 720c, 720d: micro flow channel 830: filter tanks 820a, 820b, 820c, 820d: micro flow channels A, B, C, D: end 840: filter
21twenty one