1274156 九、發明說明： 【發明所屬之技術領域】 本發明係有關一種生物碟片自動檢測方法，尤指一種 -能自動檢測不同設計的微流體生物碟片之檢測方法。 _ 【先前技術】 隨著生化科技的發展以及人類基因解碼，生物晶片正 式進入我們的生活。所謂的生物晶片，是一種微型裝置， 利用極精密的技術，根據不同需求，把大量特定生物材料 有次序的放置在一片非常微小，用紙、玻璃、矽或其他化φ 學合成物製造的載體上，可用於各種生化實驗檢測。生物 晶片最常運用在疾病了解，因為人類百分之六十以上的疾 病和基因缺陷或異常有關，透過暸解基因功能有助於了解 疾病機制，找出預防或治療的方法。因此，透過抽血、分 離、擊破、萃取、篩選與放大等一連串複雜過程，從生物 疾病帶原體中取得基因或蛋白質，再以這些基因或蛋白質 為生物材料做成生物晶片（模擬病原體）’醫療人貝便可以 用這些具有模擬病原體的生物晶片進行檢測。· 在生物晶片檢驗方面’為便於觀察檢驗結果’生物晶 片製造過程中必須在有問題的基因或蛋白質體上標記螢光 劑，在檢驗過程中只要把檢驗的樣本，例如尿液或血液滴 1在晶片上，如果發生螢光反應，就表示檢驗樣本中的基因 含有和晶片上類似的病源體5醫療人貝就可以根據晶片上 的反應判斷症狀。 習用之生物晶片之訊號檢測方法與裝置，係將生物晶 !274156 片设置於具有資料層之光碟片上，如我國專利公告第4 9 5 7 4 8號專利案即是，其係將雷射光點正癌地聚焦在光 碟片中之生物晶片上，並以一設在回光光路中之分光鏡將 该生物晶片之螢光訊號及該生物晶片所對應之光碟片的資 料層反射回來之訊號回光分離，然後分別以光债檢器將訊 就讀取出來，並藉由螢光訊號所對應的該資料層的訊號在 資料格式所對應的位址，經由資料處理器，重建該生物晶 片之螢光訊號之二維格式，便可以達到檢測生物晶片之功 效。 習用之方法，係經由讀取生物晶片之訊號以及資料層 之訊號相互比對，而重建其二維格式，所以生物晶片之設 置必需考量與資料層之相對應位置，這將導致生物晶片設 置之幵V怨雙限，且此種生物晶片之檢測裝置無法運用於其 匕形悲之生物晶片之檢測。尤其此種生物晶片之檢測裝置 其訊號之取得以及處理過程複雜，造成設備昂貴成本增加。 便有業者研發出不必設置資料層之微流體生曰物碟 片，〃係於碟片上設置多組反應區，各反應區中係包含了 存放血清、抗體等的儲水槽、數個反應檢驗槽、數個 計、廢液槽以及多數連通各槽之流道，使血清、抗體 等能藉碟片旋轉之離心力而經由流道進入反應檢驗槽中進 行生化反應，經生化反應而產生螢光或顏色排列、組合等， 2以影像擷取裝置擷取各反應檢驗槽之影像，而以機器視 覺系統辨識顏色之排列並辨識檢驗結果。 然而’ 4述之微流體生物碟片，會因進行不同之檢測 1274156 而有需要設置不同數量之反應區，且各反應區中之儲水 槽、反應檢驗槽及流道等之位置、大小及數量亦會因應而. 改變，但現有之生物碟片檢測裝置均係專為特定^生:碟 片而設計，且其所運用之檢測方法亦專為特定之生物碟片' 而量身設計，對此本發明人遂研發出一種可供檢測不同設 計之生物碟片的檢測系統’其係由相互連接之電腦、顯示 器、控制組以及光罩箱組成生物碟片'自動檢測系統，該光 罩箱中設有可作X、γ軸向位移調整之調整平台，且啁整 平台上設有-升降座’該升降座上並設有影像:貝取裝；，春 且該基座上另設有-旋轉機構，旋轉機構之旋轉轴頂端設 置一夾具’以供夾持生物碟片’而可藉由調整該調整平台 與升降座而由影像擷取裝置擷取生物碟片中反應檢驗槽之 影像來進行辨識。 而如何以前述之生物碟片的檢測系統來檢測不同設計 之生物碟片的方法便成本發明所要解決之課題。 【發明内容】 本發明之主要目的，在於解決上述的問題而提供一種_ 生物碟 自動檢測方法，其係於系統啟祕平台程式初始 化後，將生物碟片置放於旋轉機構並調整位置至反應檢驗 槽位於影像擷取裝置之影像擷取範圍中，此時旋轉機構之 伺服馬達的伺服參數會被债知而以此作為生物碟片之定 位，再口應不同之生物碟片進行反應區參數、反應檢驗槽 框選次數芩數與速度、加速度、時間參數之設定，以自動 執行檢測工作，而可達到檢測不同設計之生物碟片的功效。 1274156 為達前述之目的，本發明之生物碟片自動檢測方法， 至少包括： 提供一生物碟片自動檢測系統，其係包括一電腦、一 與電腦連接之顯示器、一與電腦連接之控制組以及—光罩 箱，該光罩箱中設有一可作X、γ、Z三軸向位移調整之 调整平台，而該調整平台上架設有一影像擷取裝置，該光 罩箱中另設有一旋轉機構，該旋轉機構係由一伺服馬達驅 動一旋轉軸，該旋轉軸之中段架設有一光源組，且該旋轉 轴之頂端則設置一夾具，以供夹持生物碟片，俾藉由該控 制組來調控調整平台、影像擷取裝置以及伺服馬達之作動； 提供一生物碟片，其係於碟片上設置數組反應區，各 反應區中係包含了數個存放血清、抗體等的儲水槽、數個1274156 IX. Description of the Invention: [Technical Field] The present invention relates to a method for automatically detecting a biological disc, and more particularly to a method for automatically detecting microfluidic biological discs of different designs. _ [Prior Art] With the development of biotechnology and the decoding of human genes, biochips are entering our lives. The so-called biochip is a micro-device that uses extremely precise technology to place a large amount of specific biological materials in a very small order, on a carrier made of paper, glass, enamel or other chemical composition, according to different needs. It can be used for various biochemical tests. Biochips are most commonly used in disease understanding because more than 60% of human diseases are associated with genetic defects or abnormalities. Understanding gene function helps to understand disease mechanisms and find ways to prevent or treat them. Therefore, through a series of complicated processes such as blood collection, separation, crushing, extraction, screening and amplification, genes or proteins are obtained from biological disease-bearing organisms, and bio-chips (simulated pathogens) are made from these genes or proteins. Medical human shells can be tested with these biofilms with simulated pathogens. · In the field of bio-wafer inspection, 'in order to facilitate the observation of the test results', the bio-wafer manufacturing process must mark the fluorescent agent on the problematic gene or protein body, and only the test sample, such as urine or blood, should be dropped during the test. On the wafer, if a fluorescence reaction occurs, it means that the gene in the test sample contains a pathogen similar to that on the wafer. 5 The medical person can judge the symptoms based on the reaction on the wafer. The method and device for detecting the signal of the biological chip used in the prior art is to set the biocrystal! 274156 piece on the optical disc with the data layer. For example, the patent of the patent publication No. 4 9 5 7 8 8 is a laser spot. Focusing on the biochip in the optical disc, and reflecting the fluorescent signal of the biochip and the data layer of the optical disc corresponding to the biochip back by a spectroscope disposed in the return optical path The light is separated, and then the signal is read by the optical debt detector, and the signal of the data layer corresponding to the fluorescent signal is reconstructed by the data processor at the address corresponding to the data format. The two-dimensional format of the fluorescent signal can be used to detect the efficacy of the biochip. The conventional method is to reconstruct the two-dimensional format by reading the signal of the biochip and the signal of the data layer, so the setting of the biochip must consider the position corresponding to the data layer, which will result in the biochip setting.幵V blame is limited, and the detection device of such a biochip cannot be applied to the detection of its biochip. In particular, the detection device of such a biochip has a complicated signal acquisition and processing process, resulting in an increase in the expensive cost of the device. Some manufacturers have developed microfluidic sputum discs that do not have to set up a data layer. The sputum system is provided with a plurality of reaction zones on the disc, and each reaction zone contains a storage tank for storing serum, antibodies, etc., and several reaction tests. The trough, the plurality of meters, the waste liquid tank and the flow passages connecting the respective tanks enable the serum, the antibody and the like to enter the reaction test tank through the flow passage to carry out the biochemical reaction by the centrifugal force of the rotation of the disc, and generate fluorescence by the biochemical reaction. Or color arrangement, combination, etc. 2 capture the image of each reaction inspection tank by the image capturing device, and identify the color arrangement by the machine vision system and identify the inspection result. However, the microfluidic biological discs described in '4' will need to set different numbers of reaction zones for different detections of 1274156, and the location, size and quantity of water storage tanks, reaction check tanks and flow passages in each reaction zone. It will also change. However, the existing bio-disc detection devices are designed for specific discs: discs, and the detection methods used are also tailor-made for specific bio-discs. The inventor of the present invention has developed a detection system for detecting biological disks of different designs, which is an automatic detection system consisting of a computer, a display, a control group and a mask box which are connected to each other. There is an adjustment platform for adjusting the axial displacement of X and γ, and a lifting platform is provided on the lifting platform. The lifting seat is provided with an image: a shell pick-up; in spring, the base is further provided with - The rotating mechanism, the top end of the rotating shaft of the rotating mechanism is provided with a clamp 'for holding the biological disc', and the image of the reaction detecting groove in the biological disc can be captured by the image capturing device by adjusting the adjusting platform and the lifting seat. Discrimination knowledge. How to detect the biological discs of different designs by the above-mentioned biological disc detection system is a problem to be solved by the invention. SUMMARY OF THE INVENTION The main object of the present invention is to solve the above problems and provide a method for automatically detecting a biological disc, which is to place a biological disc on a rotating mechanism and adjust the position to the reaction after the initialization of the system secret platform program. The inspection slot is located in the image capturing range of the image capturing device. At this time, the servo parameters of the servo motor of the rotating mechanism are known as the location of the bio-disc, and the reaction parameters of the bio-disc should be different. The response check box selects the number of turns and the setting of the speed, acceleration and time parameters to automatically perform the detection work, and can achieve the effect of detecting the biological discs of different designs. 1274156 For the purpose of the foregoing, the method for automatically detecting a biological disc of the present invention comprises at least: providing a biological disc automatic detecting system, comprising: a computer, a display connected to the computer, and a control group connected to the computer; - a mask box, wherein the mask box is provided with an adjustment platform for adjusting the three axial displacements of X, γ, and Z, and the adjustment platform is provided with an image capturing device, and a rotating mechanism is further disposed in the mask box The rotating mechanism is driven by a servo motor, a rotating shaft is arranged in the middle of the rotating shaft, and a clamp is arranged at the top of the rotating shaft for clamping the biological disc, and the control group is Adjusting and adjusting platform, image capturing device and servo motor; providing a biological disc, which is arranged on the disc to set an array reaction zone, and each reaction zone contains several storage tanks for storing serum, antibody, etc. One
反應檢驗槽、數個流量計、廢液槽以及多數連通各槽之流 道； ;,L 啟動生物碟片自動檢測系統； 平台程式初始化； 置放生物碟片：將生物碟片置放於該旋轉機構 上，放士岡敕lL ϊ 龙具 驗槽仇 伺服 W、凋整生物碟片之位置，使生物碟片之反應檢驗 ^影像擷取裝置之影像擷取範圍中，此時旋轉機構 馬達的飼服參數會㈣知，並以此作為生物碟片之定叫 凋正衫像擷取裝置：調整影像擷取装置 2， 光圈；靶圚及 設定參考點座標： ，若是則戟 載入已儲存參數··若否則進行下一步驟 1274156 參數並執行自動執行步驟； 執行參數設定：因應不同之生物碟片進行相關參數設 定，若否則結束程式； 自動執行：藉由前述之參數設定，使生物碟片自動檢 測系統自動執行檢測工作，而由影像擷取裝置擷取生物碟 片之反應檢驗槽影像，以供進行辨識； 放置新的待測生物碟片：若是則回復至平台程式初始 化步驟，若否則結束程式 結束自動生物碟片檢測系統。 本發明之上述及其他目的與優點，不難從下述所選用 實施例之詳細說明與附圖中，獲得深入了解。 當然，本發明在某些另件上，或另件之安排上容許有 所不同，但所選用之實施例，則於本說明書中，予以詳細 說明，並於附圖中展示其構造。 【實施方式】 請參閱第1圖至第3圖，圖中所示者為本發明所選用之 實施例結構，此僅供說明之用，在專利申請上並不受此種 結構之限制。 本實施例係為一種生物碟片自動檢測方法，至少包 括·· 提供一生物碟片自動檢測系統1，如第1圖所示，其 係包括一電腦1 1、一與電腦連接之顯示器1 2以及一光 罩箱2，該光罩箱2中設有一可作X、Y、Z三軸向位移 調整之調整平台2 Γ，而該調整平台2 1上架設有一影像 1274156 擷取裝置2 2，該光罩箱中另設有一旋轉機構2 3，該旋’ 轉機構2 3係由一伺服馬達2 4驅動一旋轉軸2 5，該旋 轉軸2 5之中段架設有一光源組2 6，且該旋轉軸2 5之 -頂端則設置一夾具2 7，以供夾持生物碟片3，俾藉由電 / 腦來調控調整平台2 1、影像擷取裝置2 2以及伺服馬達 2 4之作動。 提供一生物碟片3，如第2圖所示，其係於碟片上設 置數組反應區3 0，各反應區中係包含了數個存放血清、 抗體等的儲水槽3 1、數個反應檢驗槽3 2、數個流量計· 3 3、廢液槽3 4以及多數連通各槽之流道3 5。 請參閱第3圖，該自動檢測方法之步驟如下： 啟動生物碟片自動檢測系統； 平台程式初始化； 置放生物碟片：將生物碟片3置放於該旋轉機構2 3 之夾具2 7上，並調整生物碟片3之位置，使生物碟片3 之反應檢驗槽3 2位於影像擷取裝置2 2之影像擷取範圍 中，此時旋轉機構2 3之伺服馬達2 4的伺服參數會被偵 · 知，並以此作為生物碟片3之定位。 調整影像擷取裝置：調整影像擷取裝置之檢測範圍及 光圈，以使生物碟片之影像清楚呈現。 設定參考點座標： 載入已儲存參數：若否則進行下一步驟，若是則載入 參數並執行自動執行步驟； 執行參數設定：因應不同之生物碟片進行相關參數設 1274156 定 若否則結束程式。參數設定步驟係包括.· 設定生物碟片之反應區參數; 設定反應檢驗槽框選次數參數； 設定速度、加速度、時間參數。 自動執行··藉由前述之參數設定，使生物 測系統自動執行檢測工作，自動執行步驟包人^自動檢 轉反應、反應區選擇與R G B分析；亦ζ自動執行旋 (綠）、Β(藍）數值作為輪人值而求得到其&紅）、G 統轉換得到對應之輸出值，以供用來判斷I自值’經糸 像擷取裝置2 2擷取生物碟片3之反應檢驗以由影 以供進行辨識。並對影像掏取裝置所擷 =2影像， 檢驗槽影像進行分析且將結果資料呈現於#碟片反應 放置，待測生物碟片:若是則回復至2。 化步驟，若否則結束程式 平台程式初始 結束自動生物碟片檢測系統。 由於微流體生物碟片，合 設置不同數量之反應區，且二 了不同之檢測而有需要 驗槽及流道等之位置、大小二區中之儲水槽、反應檢 本發明之自動檢測方法中係於「里亦會因應而改變’因此 調整生物碟片3之位置，放生物碟片」之步驟中 裝置2 2之影像擷取範圍中，::驗槽3 2位於影像擷取 達24的伺服參數會被偵知☆捋旋轉機構23之伺服馬 位，而「執行參數設定、+並^此作為生物碟片3之定 來設定反應區表數、^驟中，會因應不同設計之碟片 檢料_:域錄與速度、加 1274156 速度、時間參數。如此一來，在「自動執行」步驟中，電 腦1 1便能藉由伺服馬達2 4之伺服參數與其它相關參數 來控制伺脈馬達2 4，使生物碟片3在旋轉預定圈數（或_ 時間）後於預定位置停止，使生物碟片中各反應區之反應-檢驗槽逐/落在影像擷取裝置2 2之影像擷取範圍中，而 可由影像擷取裝置2 2擷取反應檢驗槽3 2之影像，以進 - 行辨識、判讀’而達到自動檢測之效用。 •, 綜上所述，本發明係於生物碟片置放於旋轉機構後調 整位置至反應檢驗槽位於影像擷取裝置之影像擷取範圍春 中，使旋轉機構之伺服馬達的伺服參數被偵知而以此作為 生物碟片之定位，再因應不同之生物碟片進行反應區參 數、反應檢驗槽框選次數參數與速度、加速度、時間參數 之設定，以自動執行檢測工作，而可達到檢測不同設計之 生物碟片的功效。 以上所述實施例之揭示係用以說明本發明，並非用以 限制本發明，故舉凡數值之變更或等效元件之置換仍應隸 屬本發明之範疇。 … _ 由以上詳細說明，可使熟知本項技藝者明瞭本發明的 :可達成前述目的，實已符合專利法之規定，爰提出專利 甲請。 【圖式簡單說明】 11®係本I明所&供之生物碟片自動檢測系統的配 思圖 第2圖係本發明所提供之生物碟片之結構示意圖 12 1274156 第3圖係本發明之自動檢測方法的流程圖 【圖號說明】 (本發明部分） 生物碟片自動檢測系統1 顯示器1 2 調整平台2 1 旋轉機構2 3 旋轉軸2 5 夾具2 7 反應區3 0 反應檢驗槽3 2 廢液槽3 4 電腦1 1 光罩箱2 影像擷取裝置2 2 伺服馬達2 4 光源組2 6 生物碟片3 儲水槽3 1 流量計3 3 流道3 5Reaction test tank, several flow meters, waste liquid tanks and most flow passages connecting the tanks; ;, L start biological disc automatic detection system; platform program initialization; placing biological discs: placing biological discs on the On the rotating mechanism, the Shiggang 敕lL ϊ 具 验 验 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服 伺服The feeding parameters will be known as (4), and as a biological disc, the image is like a picking device: adjust the image capturing device 2, aperture; target and set reference point coordinates: if yes, then load Save the parameters··If otherwise proceed to the next step 1274156 parameters and execute the automatic execution steps; Execute the parameter settings: set the relevant parameters according to different biological discs, if otherwise terminate the program; Auto-execute: make the creatures by the above parameter settings The disc automatic detecting system automatically performs the detecting work, and the image capturing device captures the image of the reaction check slot of the biological disc for identification; placing a new test organism Sheet: If the program is returned to the initial step internet, or if the end of the program ends the automatic disc detection of biological systems. The above and other objects and advantages of the present invention will be readily understood from Of course, the invention may be varied on certain components, or in the arrangement of the components, but the selected embodiments are described in detail in the specification and their construction is shown in the drawings. [Embodiment] Please refer to Fig. 1 to Fig. 3, which shows the structure of the embodiment selected for the present invention, which is for illustrative purposes only and is not limited by this structure in the patent application. The embodiment is a method for automatically detecting a biological disc, and at least includes: providing a biological disc automatic detecting system 1 as shown in FIG. 1 , which comprises a computer 1 1 and a display connected to the computer 1 2 And a mask box 2, wherein the mask box 2 is provided with an adjustment platform 2 可 for X, Y, Z three-axis displacement adjustment, and the adjustment platform 2 1 is provided with an image 1274156 picking device 2 2 A rotating mechanism 23 is further disposed in the reticle box, and the rotating mechanism 2 3 is driven by a servo motor 24 to drive a rotating shaft 25, and a rotating light unit 25 is provided with a light source group 2 6 At the top of the rotating shaft 2 5 is a clamp 2 7 for holding the bio-disc 3, and the adjustment platform 2 1 , the image capturing device 2 2 and the servo motor 24 are controlled by electric/brain. A bio-disc 3 is provided. As shown in FIG. 2, an array reaction zone 30 is disposed on the disc, and each reaction zone contains a plurality of storage tanks for storing serum, antibodies, etc., and several reactions. The inspection tank 3 2, a plurality of flow meters, 3 3, a waste liquid tank 3 4 and a plurality of flow passages 35 connecting the respective tanks. Referring to FIG. 3, the steps of the automatic detecting method are as follows: starting the biological disc automatic detecting system; initializing the platform program; placing the biological disc: placing the biological disc 3 on the jig 2 of the rotating mechanism 2 3 And adjusting the position of the biological disc 3 so that the reaction check slot 3 2 of the biological disc 3 is located in the image capturing range of the image capturing device 2 2, and the servo parameters of the servo motor 24 of the rotating mechanism 23 will be Being detected, knowing, and using this as the location of the bio-disc 3. Adjusting the image capture device: Adjust the detection range and aperture of the image capture device to make the image of the biological disc clear. Set reference point coordinates: Load stored parameters: If otherwise, proceed to the next step, if yes, load the parameters and execute the automatic execution steps; Execute parameter setting: set the relevant parameters according to different biological discs. 1274156 If not, end the program. The parameter setting steps include: setting the reaction zone parameters of the bio-disc; setting the parameters of the reaction check slot selection times; setting the speed, acceleration, and time parameters. Automated execution · By the above parameter setting, the biometric system automatically performs the detection work, and automatically performs the steps of the package person ^ automatic detection reaction, reaction zone selection and RGB analysis; also automatically executes the rotation (green), Β (blue The numerical value is obtained as the round human value, and the corresponding output value is obtained by the G system, and is used for judging the I self-valued 'reaction test of the bio-disc 3 by the image capturing device 2 2 It is used for identification. And the image capture device 撷 = 2 image, the inspection slot image is analyzed and the result data is presented in the # disc reaction placement, the biological disk to be tested: if yes, return to 2. The step of the program, if otherwise terminated, the platform program initially ends the automatic bio-disc detection system. Due to the microfluidic biological discs, a different number of reaction zones are provided, and two different detections are required, such as the position of the inspection tank and the flow passage, the storage tank in the second zone, and the automatic detection method of the invention. It is in the image capturing range of the device 2 in the step of "changing the position of the bio-disc 3 and placing the bio-disc" in the process of "in the case of changing the position of the bio-disc 3":: the inspection slot 3 2 is located at the image capturing 24 The servo parameters will be detected ☆ 伺服 the servo horse position of the rotating mechanism 23, and "execution parameter setting, + and ^ as the setting of the bio-disc 3 to set the number of reaction zone tables, ^, will respond to different designs of the disc Film inspection _: field record and speed, plus 1274156 speed, time parameters. In this way, in the "automatic execution" step, the computer 1 1 can control the servo parameters and other related parameters of the servo motor 24 The pulse motor 24 stops the biological disc 3 at a predetermined position after a predetermined number of rotations (or _ time), so that the reaction-inspection tank of each reaction zone in the biological disc is dropped/falling on the image capturing device 2 2 The image capture range is captured by the image The device 2 2 captures the image of the reaction check tank 32 to perform the function of automatic detection by performing line identification and interpretation. In summary, the present invention is to adjust the position of the biological disc after being placed in the rotating mechanism until the reaction check slot is located in the image capturing range of the image capturing device, so that the servo parameters of the servo motor of the rotating mechanism are detected. Knowing this as the positioning of the bio-disc, and then responding to the parameters of the reaction zone, the parameters of the reaction check slot, and the setting of the speed, acceleration and time parameters according to different bio-discs, to automatically perform the detection work, and the detection can be achieved. The efficacy of differently designed bio-discs. The above description of the embodiments is intended to be illustrative of the invention, and is not intended to limit the scope of the invention. ... _ From the above detailed description, the person skilled in the art can understand the present invention: the foregoing objects can be achieved, and the requirements of the patent law have been met, and a patent application is filed. BRIEF DESCRIPTION OF THE DRAWINGS FIG. 2 is a schematic diagram of a biological disc provided by the present invention. 12 1274156 FIG. 3 is a schematic view of the present invention. Flowchart of automatic detection method [Illustration of the figure] (part of the invention) Biodisc automatic detection system 1 Display 1 2 Adjustment platform 2 1 Rotating mechanism 2 3 Rotary shaft 2 5 Fixture 2 7 Reaction zone 3 0 Reaction check tank 3 2 Waste tank 3 4 Computer 1 1 Photomask 2 Image capture device 2 2 Servo motor 2 4 Light source group 2 6 Bio-disc 3 Water storage tank 3 1 Flow meter 3 3 Flow path 3 5
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