TR201604548A2 - Production Method of Antimicrobial Composition by Biological Synthesis from Plant Species - Google Patents
Production Method of Antimicrobial Composition by Biological Synthesis from Plant Species Download PDFInfo
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- TR201604548A2 TR201604548A2 TR2016/04548A TR201604548A TR201604548A2 TR 201604548 A2 TR201604548 A2 TR 201604548A2 TR 2016/04548 A TR2016/04548 A TR 2016/04548A TR 201604548 A TR201604548 A TR 201604548A TR 201604548 A2 TR201604548 A2 TR 201604548A2
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- 230000000845 anti-microbial effect Effects 0.000 title claims abstract description 15
- 238000004519 manufacturing process Methods 0.000 title claims description 25
- 238000003786 synthesis reaction Methods 0.000 title description 18
- 230000015572 biosynthetic process Effects 0.000 title description 14
- 229910052709 silver Inorganic materials 0.000 claims abstract description 30
- 239000004332 silver Substances 0.000 claims abstract description 30
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims abstract description 27
- 229910052725 zinc Inorganic materials 0.000 claims abstract description 27
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Gümüş/çinko partikül, en az bir çözücü ve bitki ekstraktı içeren antimikrobiyal kompozisyon ile ilgilidir.The silver / zinc particle relates to the antimicrobial composition comprising at least one solvent and plant extract.
Description
TARIFNAME Bitki Türlerinden Biyolojik Sentezle Antimikrobiyal Kompozisyon Uretim Metodu Teknik Alan Bulus, bitkisel (biyolojik) yöntemle sentezlenen gümüs ve çinko nanopartikülün, antimikrobiyal kompozisyon üretiminde kullanimi ile ilgilidir. DESCRIPTION Antimicrobial Composition Production Method by Biological Synthesis from Plant Species Technical Area The invention shows that silver and zinc nanoparticles synthesized by herbal (biological) method, relates to its use in the manufacture of antimicrobial compositions.
Teknigin Bilinen Durumu Mevcut teknikte, dezenfektan olarak kullanilan kompozisyonlarda, kimyasal sentez yoluyla elde edilen gümüs (Ag) veya çinko (Zn) nanopartikülleri etken madde olarak kullanilmaktadir. State of the Art In the present art, compositions used as disinfectants can be synthesized by chemical synthesis. The obtained silver (Ag) or zinc (Zn) nanoparticles are used as the active ingredient.
Organik solvent veya su kullanilarak kimyasal indirgenme, gümüs nanopartiküllerinin (AgNPs) üretimindeki en yaygin tekniktir. indirgen olarak genellikle, borhidrür (borohydride), sodyum dodesil sülfat, sodyum sitrat, askorbat poliol, NaBH4, N2H4 ve N, N- dimetilformamit kullanilir. Sonuçta, kimyasal isleme maruz kalmis, çapi nano seviyesine indirgenmis kolloidal gümüs elde edilmis olur. Chemical reduction using organic solvent or water It is the most common technique for the production of AgNPs. borohydride (borohydride), usually as a reducing agent sodium dodecyl sulfate, sodium citrate, ascorbate polyol, NaBH4, N2H4 and N,N-dimethylformamide used. As a result, a colloidal chemical treated, reduced in diameter to nano level silver is obtained.
Benzer sekilde, çinko nanopartikülünün kimyasal yollarla sentezi için de, farkli kimyasal bilesenlere ihtiyaç vardir. Örnegin; NaOH, SnCIz ve etanol verilebilir. Similarly, for the synthesis of zinc nanoparticles by chemical means, different chemical components are needed. For example; NaOH, SnCl2 and ethanol may be given.
Söz konusu nanopartikülleri elde etmek için gerekli olan kimyasal indirgenme reaksiyonlarinin büyük çogunlugu, yüksek isi soku uygulamasina ihtiyaç duyar. Termal yolla kimyasal Ag +'nin eldesi için, indirgen ajan olarak m-hidroksil benzaldehit (m-hydroxy banzaldehite), hidrazin ve dekstroz ile birlikte kullanilir. Bu nedenle, nanopartikülün morfolojisi, sentez süresince uygulanan sicaklik degerinden etkilenir. Bunun yanisira, sekonder indirgen olarak sodyum formaldehit sülfoksilat da kullanilmaktadir. Baska bir yöntem de ise; glikoz ve glukonik asit kullanilmaktadir. The chemical reduction required to obtain the nanoparticles in question The vast majority of reactions require the application of high heat shock. by thermal For the production of chemical Ag+, m-hydroxyl benzaldehyde (m-hydroxyl) is used as reducing agent. banzaldehyde), hydrazine and dextrose. Therefore, the nanoparticle Its morphology is affected by the applied temperature during the synthesis. And also, Sodium formaldehyde sulfoxylate is also used as a secondary reducing agent. Another in the method; glucose and gluconic acid are used.
Alternatif yöntemler arasinda, etilen glikoI-su karisiminin kullanildigi X-ray isimasina maruz birakma yontemi sayilabilir. Bu yöntem de, UV isin kullanilarak, nanopartikül eldesi yapilmaktadir. Ozellikle, fabrikasyon gümüs nanopartiküllerinin sentezi için, 720 C“ye ayarlanmis firinlarda, yüksek sicaklik ve basinçla gerçeklestirilen pyrolis teknigi de bilinen bir diger ybntemdir. Bu yöntem için Ag/MgO karisimina ihtiyaç olup, son asamada lVlgO in uzaklastirilmasi için amonyum klorür (NH4CI) solüsyonu kullanilir. Alternative methods include exposure to X-ray, in which an ethylene glycol-water mixture is used. can be counted as a release method. In this method, nanoparticles are obtained by using UV light. is being done. In particular, for the synthesis of fabricated silver nanoparticles, the temperature to 720 °C The pyrolis technique, which is carried out with high temperature and pressure, is a well-known is the other module. For this method, Ag/MgO mixture is needed, and in the final stage IVlgO in For removal, ammonium chloride (NH4Cl) solution is used.
Mevcut bilinen yöntemlerle üretilen kompozisyonlarda, yukari açiklanan tekniklerden birisi veya bir kaçi ile elde edilen gümüs nanopartikülü bulunmaktadir. Ayrica, mevcut yöntemlerde, kimyasal sentez gümüs veya çinko nanopartiküllerinin stabilizasyonu için surfaktan maddenin sentezde ortama eklenmesi gerekmektedir. In compositions produced by currently known methods, one of the techniques described above or a few silver nanoparticles. Also, available chemical synthesis for the stabilization of silver or zinc nanoparticles The surfactant must be added to the medium in the synthesis.
Literatürde yapilan arastirmalar sirasinda, konu ile ilgili karsimiza çikan basvurulardan biri; TR201314809 yayin numarali patent basvurusudur. Bulus; depolamada kararli sulu bir konsantrata iliskin olup, bir dezenfektan hazirlamasi ile ilgilidir. Konsantrat; konsantrata göre hacim itibariyle %30 ile %70 arasindaki bir konsantrasyonda hidrojen peroksit; nihai konsantrata göre agirlik itibariyle 150 ile 1000 ppm arasindaki bir konsantrasyonda kolloidal gümüs; arap zamki, jelatin, guar sakizi, karragenen, pektin ve bunlarin kombinasyonlarindan olusan gruptan seçilen bir stabilizatör içermektedir. etanol, izopropil alkol veya bunlarin bir karisimi; (b) %01 ila %2 (ala) Iaktik asit; (c) %001 ila çinko içeren bir bilesikten olusmaktadir. During the researches in the literature, one of the applications related to the subject; It is a patent application with the publication number TR201314809. Meet; a stable aqueous solution in storage relates to the concentrate and relates to the preparation of a disinfectant. concentrate; by concentrate hydrogen peroxide at a concentration of 30 to 70% by volume; final colloidal at a concentration of 150 to 1000 ppm by weight, relative to the concentrate. silver; gum arabic, gelatin, guar gum, carrageenan, pectin and combinations thereof comprising a stabilizer selected from the group consisting of: ethanol, isopropyl alcohol, or a mixture thereof; (b) 01 to 2% (ala) lactic acid; (c) 001% to It consists of a zinc-containing compound.
Dolayisiyla, mevcut yöntem kompozisyonlarinin, insan ve çevre sagligi açisindan riskli olabilecek sentetik, kimyasal kökenli maddelerle desteklenmesi bir dezavantajdir. Bunun yani sira, kimyasal sentez gümüs ve çinko nanopartiküllerinin, uygulama için gereken kimyasala bagli olarak maliyetinin, diger bir deyisle enerji gereksiniminin degismesi de, bir diger dezavantajd ir. Therefore, the present method compositions are risky for human and environmental health. It is a disadvantage that it is supported with synthetic, chemical origin substances that may be That's it Next, the chemical synthesis of silver and zinc nanoparticles depends on the chemical required for application. Depending on the cost, the change in the energy requirement, in other words, is another factor. is disadvantaged.
Sonuç olarak yukarida anlatilan olumsuzluklardan dolayi ve mevcut çözümlerin konu hakkindaki yetersizligi nedeniyle, insan basta olmak üzere canli ve çevre sagligina zarar vermeyecek sekilde dezenfektan üretim metodu ile ilgili teknik alanda bir gelistirme yapilmasi gerekli kilinmistir. As a result, due to the above-mentioned negativities and existing solutions damage to living and environmental health, especially human, due to its inadequacy about Making an improvement in the technical field related to the disinfectant production method in a way that will not give is necessary.
Bulusun Amaci Mevcut bulus, yukarida bahsedilen gereksinimleri karsilayan, tüm dezavantajlari ortadan kaldiran ve ilave bazi avantajlar getiren bitki türlerinden biyolojik sentezle antimikrobiyal kompozisyon üretim metodu ile ilgilidir. Purpose of the Invention The present invention satisfies the above-mentioned requirements, eliminates all disadvantages. antimicrobial by biological synthesis from plant species that remove The composition relates to the production method.
Bulusun öncelikli amaci, bitkisel yöntemle sentezlenen gümüs ve çinko nanopartikül içermesidir. The primary aim of the invention is to produce silver and zinc nanoparticles synthesized by herbal method. it contains.
Bulusun bir amaci, bitkisel yöntemle sentezlenen gümüs ve çinko nanopartiküllerinin, içinde kimyasal madde bulundurmamasi, cilde ve insanin temasta oldugu her türlü ortama rahatlikla uygulanabilir olup, çevre dostu olmasidir. It is an object of the invention that silver and zinc nanoparticles synthesized by the herbal method, in It does not contain chemicals, it can be easily applied to the skin and any environment that people come into contact with. applicable and environmentally friendly.
Bulusun bir diger amaci, antimikrobiyal kompozisyon üretimi için gerekli olan gümüs ve çinko nanopartiküllerinin eldesi için indirgen madde olarak, bitki ekstrakti kullanilmasidir. Bu sayede; mevcut teknikteki gibi, kimyasal maddelerin indirgen ve stabilizat'or olarak kullanimina gereksinim kalmamistir. Another object of the invention is the silver and zinc required for the production of the antimicrobial composition. To obtain nanoparticles, plant extract is used as a reducing agent. This by this means; As in the current art, chemical substances can be used as reducing and stabilizing agents. there is no need for its use.
Bulusun bir baska amaci, mevcut teknikteki kimyasal sentez yerine, biyolojik sentezle üretim yapilmasinin; patojen mikroplarin ortamdan giderilmesinde, zaman, üretim maliyeti ve enerji gereksinimlerini azaltmasi, üretim asamalarini kolaylastirmasidir. Another object of the invention is production by biological synthesis instead of chemical synthesis in the current art. to be made; time, production cost and energy in removing pathogenic microbes from the environment. reducing the requirements, facilitating the production steps.
Bulusun bir benzer amaci, bitki ekstrakti olarak, gilaburu bitkisi kullanilmasidir. Gilaburu bitkisinin kullanilmasiyla elde edilen gümüs veya çinko nanopartikül içeren kompozisyonun, gram pozitif ve gram negatif bakteri türleri, virüsler ile mantarlar üzerinde antimikrobiyal, antiviral ve antifungal etkenlige sahip olmasidir. A similar object of the invention is the use of ginseng as a plant extract. Gilaburu The composition containing silver or zinc nanoparticles obtained by using the plant, antimicrobial on gram positive and gram negative bacteria species, viruses and fungi, It has antiviral and antifungal activity.
Bulusun bir diger amaci, gilaburu ekstraktindan sentezlenen gümüs ve çinko nanopartikülleri ile yapilan ekotoksisite çalismalarinda, çalisilan sucul canlilarda toksik etkilere yol açmamasidir. Another object of the invention is silver and zinc nanoparticles synthesized from gilaburberry extract. In ecotoxicity studies with it doesn't open.
Yukarida anlatilan amaçlarin yerine getirilmesi için, gümüs/çinko partikül ve en az bir çözücü içeren antimikrobiyal kompozisyon olup, Özelligi; bitki ekstrakti ihtiva etmektedir. To fulfill the above-described purposes, the silver/zinc particle and at least one solvent It is an antimicrobial composition containing; Contains plant extract.
Bulusun yapisal ve karakteristik özellikleri ve tüm avantajlari asagida verilen sekiller ve bu sekillere atiflar yapilmak suretiyle yazilan detayli açiklama sayesinde daha net olarak anlasilacaktir ve bu nedenle degerlendirmenin de bu sekiller ve detayli açiklama göz önüne alinarak yapilmasi gerekmektedir. The structural and characteristic features of the invention and all its advantages are given in the following figures and More clearly thanks to the detailed explanation with references to the figures. will be understood and therefore the assessment should also take into account these figures and detailed explanation. must be made by taking
Bulusun Anlasilmasina Yardimci Olacak Sekiller Sekil 1: Bulus konusu gümüs nanopartikülüne ait SEM görüntüsüdür. Figures to Help Understand the Invention Figure 1: SEM image of the silver nanoparticle which is the subject of the invention.
Sekil 2: Bulus konusu çinko nanopartikülüne ait SEM görüntüsüdür. Figure 2: SEM image of the subject of the invention zinc nanoparticle.
Sekil 3:Bulus konusu bitkisel sentez ile nanogümüs içeren solüsyonun Staphylococcus aureus (a) ve Escherichia coli (b)”de olusturdugu zonlar Sekil 4: Bulus konusu bitkisel sentez ile nanoçinko içeren solüsyonun Staphylococcus aureus (a) ve Escherichia coli (byde olusturdugu zonlar Sekil 5a: NanoGümüs içeren karisimin degisen oranlarinda Lemna gibbaida agirlik degisimi grafigidir (Ekotoksisite testi) Sekil 5b: NanoÇinko içeren karisimin degisen oranlarinda Lemna gibba'da agirlik degisimi grafigidir (Ekotoksisite testi) Sekil 6a: NanoGümüs içeren karisimin degisen oranlarinda Elocharis aciculariside agirlik degisimi grafigidir (Ekotoksisite testi) Sekil 6b: NanoÇinko içeren karisimin degisen oranlarinda Elocharis acicularis'de agirlik degisimi grafigidir (Ekotoksisite testi) Sekil 7a: NanoGümüs içeren karisimin degisen oranlarinda L.gibba ve E.acicularis=de spesifik büyüme orani grafigidir (Ekotoksisite testi) Sekil 7b: NanoÇinko içeren karisimin degisen oranlarinda L.gibba ve E.aciculariside spesifik büyüme orani gratigidir (Ekotoksisite testi) Çizimlerin mutlaka ölçeklendirilmesi gerekmemektedir ve mevcut bulusu anlamak için gerekli olmayan detaylar ihmal edilmis olabilmektedir. Bundan baska, en azindan büyük Ölçüde Özdes olan veya en azindan büyük ölçüde özdes islevleri olan elemanlar, ayni numara ile gösterilmektedir. Figure 3: Plant synthesis of the subject of the invention and the solution containing nanosilver for Staphylococcus aureus (a) and Escherichia coli (b) Figure 4: Plant synthesis and nanozinc-containing solution of the invention subject to Staphylococcus aureus (a) and Escherichia coli (zones formed by by Figure 5a: Lemna gibbai weight change at varying proportions of NanoSilver containing mixture is the graphic (Ecotoxicity test) Figure 5b: Weight change in Lemna gibba at varying proportions of NanoZinc containing mixture is the graphic (Ecotoxicity test) Figure 6a: Weight of Elocharis aciculariside in varying proportions of the mixture containing NanoSilver is the graph of the change (Ecotoxicity test) Figure 6b: Weight in Elocharis acicularis at varying proportions of mixture containing NanoZinc is the graph of the change (Ecotoxicity test) Figure 7a: In varying proportions of the mixture containing NanoSilver, L.gibba and E.acicularis=de is the specific growth rate graph (Ecotoxicity test) Figure 7b: L.gibba and E.aciculariside specificity in varying proportions of the mixture containing NanoZinc is the growth rate graph (Ecotoxicity test) Drawings do not necessarily need to be scaled and are necessary to understand the present invention. details may be neglected. Moreover, at least to a large extent Elements that are identical, or at least substantially identical, are assigned the same number. is shown.
Bulusun Detayli Açiklamasi Bu detayli açiklamada, bulus konusu bitki türlerinden biyolojik sentezle antimikrobiyal kompozisyon üretim metodunun tercih edilen yapilanmalari, sadece konunun daha iyi anlasilmasina yönelik olarak ve hiçbir sinirlayici etki olusturmayacak sekilde açiklanmaktadir. 0 saglik sektöründe; ameliyathanelerde kullanilan malzemelerin dezenfeksiyonunda, - dis hekimligi ve dis hastanelerinde; implant ve protez yüzeylerin dezenfeksiyonunda, o AVM, hastane, stadyum gibi 'Özel ve kamusal binalarin ortak kullanim alanlarinin, çocuk oyun alanlarinin sterilizasyonunda (sosyal alanlarda, okul, kres, anasinifi, spor salonlari, oteller, isyerleri ve ibadethane dezenfeksiyonunda), . toplu tasima ve bireysel araçlarin dezenfeksiyonunda, o asansörler, tramvay ve otobüs tutamaklari, hastane, okul ve isyeri kapi kollarinin dezenfeksiyonunda, o restoran ve kafelerin masa ve koltuklarinin dezenfeksiyonunda, o evlerde; yasam alanlarinin ve cep telefonu, bilgisayar gibi kisisel araçlarin ve kisisel temizlik malzemelerin sterilizasyonunda, . iklimlendirme ve havalandirma sistemlerinin dezenfeksiyonunda, - ilaç endüstrisinde; mevcut antibiyotiklere dirençli bakterilere karsi, 0 gida sektöründe; ambalajlama ve gida hijyeninin saglanmasinda, - dolumu yapilan içeceklerin ve ambalajlarinin ve tesislerinin sterilizasyonunda, o ilaç üretim tesislerinin ve malzemelerinin sterilizasyonunda, - ilaç üretim tesislerinde çalisan personellerin kiyafetlerinin sterilizasyonunda, 0 uydu üretim tesislerinin ve uydu üretim tesislerinde çalisan personelin kiyafetlerinin sterilizasyonunda, . kozmetik sanayi ürünlerinin (makyaj malzemeleri, krem, losyon, jel) antimikrobiyal etkinliginin saglanamasinda, . yüksek teknoloji ile üretim yapan mikroçip, islemci, anakart, LCD ekran, entegre devre ve benzeri tesislerin sterilizasyonunda kullanilmak üzere; bitkisel (biyolojik) yöntemle sentezlenen gümüs veya çinko nanopartikül içeren antimikrobiyal kompozisyon üretim metodudur. Detailed Description of the Invention In this detailed description, antimicrobial synthesis by biological synthesis from the plant species of the invention. preferred embodiments of the composition production method only for understanding and without any limiting effect is explained. 0 in the health sector; disinfection of materials used in operating rooms, - in dentistry and dental hospitals; disinfection of implant and prosthetic surfaces, o 'Common use areas of private and public buildings such as shopping malls, hospitals, stadiums, sterilization of children's playgrounds (social areas, school, kindergarten, sports halls, hotels, workplaces and places of worship), . in the disinfection of public transport and individual vehicles, o elevators, tram and bus handles, hospital, school and office door handles in disinfection, o in the disinfection of tables and seats of restaurants and cafes, in those houses; living spaces and personal tools such as mobile phones, computers and personal sterilization of cleaning materials, . disinfection of air conditioning and ventilation systems, - in the pharmaceutical industry; against bacteria resistant to existing antibiotics, 0 in the food sector; packaging and ensuring food hygiene, - sterilization of filled beverages and their packaging and facilities, o sterilization of pharmaceutical production facilities and materials, - sterilization of the clothes of the personnel working in the pharmaceutical production facilities, 0 the clothing of the satellite production facilities and the personnel working in the satellite production facilities. in sterilization, . antimicrobial products of cosmetic industry products (make-up materials, cream, lotion, gel) in ensuring its effectiveness, . microchip, processor, motherboard, LCD screen, integrated circuit and to be used in the sterilization of similar facilities; Antimicrobial containing silver or zinc nanoparticles synthesized by herbal (biological) method composition production method.
Bulusun bir özelligi; gümüs ve çinko nanopartiküllerinin eldesi için indirgen madde olarak bitki ekstrakti kullanilmasidir. Bu sayede, mevcut teknikteki gibi, kimyasal maddelerin indirgen ve stabilizatör olarak kullanimi ortadan kalkmistir. Bu durum; içinde kimyasal madde bulundurmayan, cilde ve insanin temasta oldugu her türlü ortama rahatlikla uygulanabilen çevre dostu bir ürün ortaya çikarmistir. A feature of the invention; plant as reducing agent for the production of silver and zinc nanoparticles the use of the extract. In this way, as in the current technique, reducing and Its use as a stabilizer has been eliminated. This situation; chemical substance in It can be easily applied to the skin and any environment in which people come into contact. has created an environmentally friendly product.
BquS konusu kompozisvonun içerigi; Hammadde Agirlikça Tercih Edilen Agirlikça Kullanilabilir Miktar Miktar (%1 % Bitki ekstrakti 10 9-10 Distile su 89,92 88-91 Gümüs; fotograf sanayii, elektronik, para imali, süs esyasi ve taki yapimi, alasimlar, disçilikte yaygin olarak kullanilir. Ayrica, yapay yagmur yagdirmada, ayna sirlarinin yapiminda, bilgisayar röle kontaklarinda, pil yapiminda da kullanilmaktadir. Saf gümüs, ayni zamanda asetik asit, boyalar ve fotograf maddeleri elde etmede de kullanilir. Keza toz halinde gümüs, cam ve ahsabi, elektrik iletkeni yapmak için yeni seramik tipi kaplama islerinde kullanilmaktadir. The content of the composition that is the subject of BquS; Preferred by Weight of Raw Material and Usable Amount by Weight Amount (1% % Plant extract 10 9-10 Distilled water 89.92 88-91 Silver; in the photography industry, electronics, money making, ornaments and jewelry making, alloys, dentistry widely used. Also, in making artificial rain, making mirror glazes, It is also used in computer relay contacts and battery making. Pure silver, also Acetic acid is also used to obtain dyes and photographic materials. Likewise, silver in powder form, in new ceramic-type coating works to make glass and wood electrical conductors is used.
Gümüs çok güçlü bir antibakteriyel olup, toksik etkisi çok azdir. Bu amaçla, tip ve tekstil alaninda fazlasiyla kullanilir. Çinko; sulu boyalarda beyaz pigment olarak ve lastik sanayiinde aktivatör olarak kullanilir. Silver is a very strong antibacterial and has very little toxic effect. For this purpose, type and textile used extensively in the field. Zinc; It is used as a white pigment in watercolors and as an activator in the rubber industry.
Bazi merhemlerin bilesiminde bulunur ve ince bir tabaka halinde uygulandiginda, cildin su kaybetmesini önler. Yazin günes, kisin da soguk yaniklarina karsi koruyucudur. Bebeklerin bez baglanan bölgelerinde kizarikliklara karsi kullanilir. Çinko klorür, deodorantlarda ve ahsap koruyucu olarak kullanilir. Çinko, pek çok günlük vitamin ve mineral ilaçlarinin bilesenidir. Gümüs nanopartikülleri antibakteriyel etkilidir. It is found in the composition of some ointments and when applied as a thin layer, the skin is hydrated. prevents loss. It is protective against the sun in summer and cold burns in winter. of babies It is used against redness in the areas where the cloth is attached. Zinc chloride is used in deodorants and It is used as a wood preservative. Zinc is a component of many daily vitamin and mineral medications. is the component. Silver nanoparticles are antibacterial.
Bitkisel özlü qümüs veya çinko nanopartikül içeren antimikrobival bir kompozisyonun üretim metodu: - Bitkinin toplanmasi, - toplanan bitkinin (meyvelerin) yikanip, 70°C deki firinda, 3-4 saat süreyle kurutulup toz haline getirilmesi, - akabinde, 10 gr kuru bitki örnegi, 100 ml distile su içerisinde yaklasik 30 dakika kaynatilmasi, - süre sonunda sogumaya birakilmasi ve filtre kagidindan süzülmesi ile bitki ekstraktinin elde edilmesi, o iyonik gümüsün veya çinkonun, distile suyla karistirilip seyreltilmesi, o nanoçinko veya nanogümüse indirgenmeyi saglamak üzere; seyreltilen solüsyona, elde edilen bitki ekstraktinin ilave edilmesi, (Solüsyon ile bitki ekstrakti 9:1 oraninda karistirildi. Elde edilen karisim, cam malzeme içerisinde ve etrafi isikla temasi önlemek adina alimünyum folyo ile kapatilarak; nanoçinko eldesi için, 80°C de, renk degisimi gözleninceye kadar; nanogümüs eldesi için ise, oda sicakliginda, renk degisimi gözleninceye kadar karistirildi) (Sekil 1 ve 2), . elde edilen ürünün, saklama kaplarina alinarak, +4°C de muhafaza edilmesidir. Production of an antimicrobial composition containing herbal extracts of silver or zinc nanoparticles method: - Gathering the plant, - The collected plant (fruits) are washed and dried in the oven at 70°C for 3-4 hours. pulverizing, - followed by 10 g of dried herb sample in 100 ml of distilled water for approx. 30 minutes boiling, - the plant is left to cool at the end of the period and filtered through filter paper. obtaining the extract, o dilution of ionic silver or zinc with distilled water, o to reduce to nanozinc or nanosilver; to the diluted solution, adding the obtained plant extract, (The solution and the plant extract were mixed at a ratio of 9:1. The resulting mixture was glass material. In order to prevent light contact inside and around, by covering with aluminum foil; nanozinc for obtaining, at 80°C, until a color change is observed; For nanosilver production, room temperature, stirred until a color change was observed) (Figures 1 and 2), . The product obtained is taken into storage containers and kept at +4°C.
Bulusta dikkat edilmesi gerekenler; uygulama yapilmadan önce, ürün kutusunun çalkalanmasi ve spreyin sikilarak veya damlatilarak uygulanmasidir. Uygulama sonrasi ise, kurumasi beklenmelidir. Üç ay etkinligi olup, gerektikçe tekrarlanir. Things to consider in the invention; before application, the product box Shaking and applying the spray by squeezing or dripping. After application, should be allowed to dry. It is effective for three months and is repeated as needed.
Bulusun tercih edilen yapilanmasinda, gilaburu bitkisi kullanilmaktadir. In the preferred embodiment of the invention, the gilabur plant is used.
Gilaburu bitkisinin kullanilmasiyla elde edilen, gümüs veya çinko nanopartükül içeren kompozisyon, gram pozitif ve gram negatif bakteri türleri ile bazi mantarlar üzerinde antimikrobiyaI/antifungal etkenlige sahiptir. (Sekil 3, 4). Containing silver or zinc nanoparticles, obtained by using the Gilaburu plant The composition is effective on gram-positive and gram-negative bacteria and some fungi. It has antimicrobial/antifungal activity. (Fig. 3, 4).
Gilaburu ekstraktindan sentezlenen gümüs ve çinko nanopartikülleri ile yapilan ekotoksisite çalismalarinda, çalisilan sucul canlilarda toksik etkilere yol açmadigi deneysel olarak kanitlanmistir. (Sekil 5-7) Gilaburu, basta böbrek hastaliklari olmak üzere, birçok hastaligin tedavisinde kullanilmaktadir. Kaynatilan gilaburu; astim, romatizma, yüksek tansiyon, sara nöbetleri (epilepsi), kabakulak, dogum sonrasi spazmlar, uyku bozuklugu gibi birçok hastaligin tedavisinde kullanilabilmektedir. Gilaburu suyu, ayrica safra kesesi hastaliklari ile bazi karaciger hastaliklarina da iyi gelmektedir. Gilaburunun bazi kanser tümörlerini azaltmadaki olumlu etkileriyle ilgili çalismalar da mevcuttur. Ecotoxicity with silver and zinc nanoparticles synthesized from Gilaburu extract In their studies, it has been experimentally proven that it does not cause toxic effects on the aquatic organisms studied. has been proven. (Figure 5-7) Gilaburu is used in the treatment of many diseases, especially kidney diseases. is used. Boiled gilaburu; asthma, rheumatism, high blood pressure, epileptic seizures (epilepsy), mumps, postpartum spasms, sleep disorder, etc. can be used in the treatment. Gilaburu juice, also with some gall bladder diseases It is also good for liver diseases. The effect of gilabur in reducing some cancer tumors There are also studies on its positive effects.
Deneysel Veriler: Sekil 1 de, bulus konusu gümüs nanopartikülüne ait SEM görüntüsü; Sekil 2 de ise, bulus konusu çinko nanopartikülüne ait SEM görüntüsü bulunmaktadir. Sekil 3 de, bulus konusu bitkisel sentez ile nanogümüs içeren solüsyonun Staphylococcus aureus (a) ve Escherichia coli (b)'de olusturdugu zonlar; Sekil 4 de ise, bitkisel sentez ile nanoçinko içeren solüsyonun Staphylococcus aureus (a) ve Escherichia coli (b)'de olusturdugu zonlar bulunmaktadir. Sekil 5a da, nanoGümüs içeren karisimin degisen oranlarinda Lemna gibba'da agirlik degisimi grafigi (Ekotoksisite testi); Sekil 5b de, nanoçinko içeren karisimin degisen oranlarinda Lemna gibba=da agirlik degisimi grafigi (Ekotoksisite testi); Sekil 6a da, nanoGümüs içeren karisimin degisen oranlarinda Elocharis aciculariside agirlik degisimi grafigi (Ekotoksisite testi); Sekil Bb de, nanoçinko içeren karisimin degisen oranlarinda Elocharis aciculariside agirlik degisimi grafigi (Ekotoksisite testi); Sekil ?a da, nanoGümüs içeren karisimin degisen oranlarinda L.gibba ve Eacicularisrde spesifik büyüme orani grafigi (Ekotoksisite testi); Sekil büyüme orani grafigi (Ekotoksisite testi) yer almaktadir. Experimental Data: In Figure 1, SEM image of the silver nanoparticle, which is the subject of the invention; In Figure 2, the invention There is an SEM image of the subject zinc nanoparticle. In Figure 3, the subject of the invention herbal synthesis with nanosilver containing solution for Staphylococcus aureus (a) and Escherichia coli (b) zones; Figure 4 shows how the solution containing nanozinc was obtained by herbal synthesis. There are zones created by Staphylococcus aureus (a) and Escherichia coli (b). Shape Weight change in Lemna gibba at varying proportions of mixture containing nanoSilver at 5a graph (Ecotoxicity test); Figure 5b shows the varying proportions of the nanozinc-containing mixture. weight change graph in Lemna gibba (Ecotoxicity test); In Figure 6a, nanoSilver containing graph of weight change of Elocharis aciculariside at varying proportions of the mixture (Ecotoxicity test); In Figure Bb, varying proportions of the nanozinc-containing mixture are Elocharis aciculariside. weight change graph (Ecotoxicity test); Fig. graph of specific growth rate of L.gibba and Eacicularisr (Ecotoxicity test); Shape growth rate graph (Ecotoxicity test).
Ekotoksisite testleri, EPA (OCSPP kapsaminda yapilmistir. Testlerde Lemna gibba ve Elocharis acicularis biyolojik materyal olarak kullanilmistir. Prosedürlerde belirtildigi sekilde test süresi sonunda canli birey sayisi, ortalama spesifik büyüme egrisi, fizyolojik gözlemlerle birlikte hesaplanip degerlendirilmistir. Ecotoxicity tests, EPA (under OCSPP) has been made. Lemna gibba and Elocharis acicularis were used as biological materials in the tests. used. The number of viable individuals at the end of the test period as specified in the procedures, mean specific growth curve was calculated and evaluated together with physiological observations.
Ekotoksisite testlerinde; sucul ortam canlilarindan örnekler seçilmistir. Bu çalismalarda, numuneden eklenerek, toplam vitabilite (canlilik), yas ve kuru agirlik degisimi, klorosiz ve spesifik büyüme oranlari hesaplanmistir. Yas ve kuru agirlik tayinleri test süresinin baslangicindaki ve sonundaki bitki agirliklarini ifade etmektedir. Spesifik büyüme orani ise asagidaki formüle göre hesaplanmistir. In ecotoxicity tests; Samples from aquatic environment creatures were selected. In these studies, by adding from the sample, total viability (vitality), wet and dry weight change, chlorosis and specific growth rates were calculated. The wet and dry weight determinations of the test period refers to the plant weights at the beginning and end. If the specific growth rate is calculated according to the formula below.
Esitlikte verilen t2; deneyin süresini, M2 deney süresi sonundaki M1 ise baslangiçtaki agirlik ölçümünü ifade etmektedir. t2 given in the equation; the duration of the experiment, M2 at the end of the test period, and M1 at the beginning. stands for measurement.
Herhangi bir maddenin toksisite gösterip göstermedigini anlayabilmek için, p0pülasyondaki agirlik artisi, popülasyonda çogalma orani gibi parametrelere de bakilir. In order to understand whether any substance is toxic or not, the Parameters such as weight gain and proliferation rate in the population are also checked.
Grafikler incelendiginde, Lemna gibba ve Elocharis acicularisrde test süresi sonunda bitki agirliginda artis oldugu gözlenmistir. RGR hesaplamalari da bunu dogrular tarzdadir. When the graphs are examined, it can be seen that Lemna gibba and Elocharis acicularisr plant plants at the end of the test period. An increase in weight was observed. RGR calculations also confirm this.
Morfolojik gözlemlerde de bireylerin saglikli görünüsleri dikkat çekmistir. Sonuç olarak, bitkisel içerikli bu karisimin sucul ekosistemler için olusturmadigi söylenebilir.In the morphological observations, the healthy appearance of the individuals drew attention. As a result, it can be said that this mixture with herbal content does not form for aquatic ecosystems.
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