SU685285A1 - Medium for freezing and diluting animal sperm - Google Patents
Medium for freezing and diluting animal spermInfo
- Publication number
- SU685285A1 SU685285A1 SU782608989A SU2608989A SU685285A1 SU 685285 A1 SU685285 A1 SU 685285A1 SU 782608989 A SU782608989 A SU 782608989A SU 2608989 A SU2608989 A SU 2608989A SU 685285 A1 SU685285 A1 SU 685285A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- medium
- freezing
- animal sperm
- diluting
- gum arabic
- Prior art date
Links
Description
(54) СРЕДА ДЛЯ РАЗБАВЛЕНИЯ И ЗАМОРАЖИВАНИЯ(54) MEDIUM FOR DILUTION AND FREEZING
сшрмы давотныхdavitnyh
Изобретение относитс к сельскому хоз йству, в частности к искусственному осеменению сельскохоз йственных животных. Из описанных в литературе синтетических сред, используемых дл р азбавле ни и замораживани спермы сельскохоз йственных животных по составу ингредиентов наиболее близка к предлагаемой среда, ксутора содержит лактозу, желток куриного йца, глицерин и гуммиарабик при следующем соотношении ингредиентов , вес.%: Лактоза7,5-9,5 Желток куриного йца.10,0-20,0 Глицерин2,0-5,0 Гуммиарабик0,5-5,0 Вода дистиллированна Остальное Однако эта среда обладает недостато ным холодозащитным действием из-за отсутстви в ее составе буферных ингре диентов, вследствие чего сперма, разбав ленна и замороженна в этой среде, имеет недостаточно высокую подвижность и оплодотвор ющую способность после оттаивани . Цель изобретенЕш - создание такой среды, котора обладала бы повыщенными буферными и холодозащитны свойствами при глубоком замораживании спермы. Дл достижени этой цели в предлагаемую лактозо желточно-гуммиарабико- вую среду, в состав которой вход т лактоза , желток куриного йца, глицерин, гуммиарабик и дистиллированна вода, дополнительно ввод т в качестве буферных ве- QiSGTB трис (оксиметил)- минометан и лимонную кислоту, при этом содержание всех указанных ингредиентов должно быть в следующих соотношени х, вес.%: Дисахарид, например лактоза8,5-9,5 Желток куриного йца 10,0-20,0 Глицерин7,5-11,0 Гуммиарабик2,0-6,0The invention relates to agriculture, in particular to the artificial insemination of farm animals. Of the synthetic media described in the literature used to dilute and freeze semen of farm animals, the composition of ingredients is closest to the proposed medium, xutora contains lactose, chicken egg yolk, glycerin and gum arabic in the following ratio of ingredients, wt.%: Lactose7.5 -9.5 Chicken Egg Yolk.10.0-20.0 Glycerin2.0-5.0 Gum arabic 0.5-5.0 Distilled water Else However, this environment has a poor cold-protective effect due to the absence of buffer ingredients in its composition following Because of this, semen, diluted and frozen in this medium, does not have a sufficiently high mobility and fertilizing capacity after thawing. The goal of the invention is to create such an environment that would have enhanced buffer and cold-protection properties during deep freezing of sperm. To achieve this goal, the proposed lactose-yolk-gum arabic medium, which includes lactose, egg yolk, glycerin, gum arabic, and distilled water, is additionally introduced as a buffer, QiSGTB tris (hydroxymethyl) minomethane and citric acid , while the content of all of these ingredients should be in the following ratios, wt.%: Disaccharide, for example lactose8,5-9,5 Yolk of chicken eggs 10.0-20.0 Glycerol 7.5-11.0 Gum arabic 2.0-6 , 0
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU782608989A SU685285A1 (en) | 1978-04-06 | 1978-04-06 | Medium for freezing and diluting animal sperm |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU782608989A SU685285A1 (en) | 1978-04-06 | 1978-04-06 | Medium for freezing and diluting animal sperm |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU685285A1 true SU685285A1 (en) | 1979-09-15 |
Family
ID=20761728
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU782608989A SU685285A1 (en) | 1978-04-06 | 1978-04-06 | Medium for freezing and diluting animal sperm |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU685285A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997014785A3 (en) * | 1995-10-19 | 1997-09-12 | Advanced Reproduction Technolo | Methods and compositions to improve germ cell and embryo survival and function |
-
1978
- 1978-04-06 SU SU782608989A patent/SU685285A1/en active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997014785A3 (en) * | 1995-10-19 | 1997-09-12 | Advanced Reproduction Technolo | Methods and compositions to improve germ cell and embryo survival and function |
| US6140121A (en) * | 1995-10-19 | 2000-10-31 | Advanced Reproduction Technologies, Inc. | Methods and compositions to improve germ cell and embryo survival and function |
| US6593309B2 (en) | 1995-10-19 | 2003-07-15 | Bio-Origyn Llc | Methods and compositions to improve germ cell and embryo survival and function |
| US7838509B2 (en) | 1995-10-19 | 2010-11-23 | Bio-Origyn Llc | Methods and compositions to improve germ cell and embryo survival and function |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Salamon et al. | Effect of composition of Tris-based diluent and of thawing solution on survival of ram spermatozoa frozen by the pellet method | |
| Burch | The caribou/wild reindeer as a human resource | |
| Kerby | Cryogenic preservation of sperm from striped bass | |
| Chappell et al. | Dependence of mitochondrial swelling on oxidizable substrates | |
| Setchell et al. | Epididymal constituents and related substances in the storage of spermatozoa: a review | |
| US3444039A (en) | Freezable live cell diluent and process | |
| SU685285A1 (en) | Medium for freezing and diluting animal sperm | |
| Sexton | Development of a Commercial Method for Freezing Turkey Semen.: I. Effect of Prefreeze Techniques on the Fertility of Processed Unfrozen and Frozen-Thawed Semen | |
| Sexton | A new poultry semen extender: 5. Relationship of diluent components to cytotoxic effects of dimethylsulfoxide on turkey spermatozoa | |
| Brown et al. | The influence of glycerol equilibration time on the metabolism, motility and fertility of frozen chicken spermatozoa | |
| Olson | Ontogeny of catabolic and morphological properties of skeletal muscle of the red-winged blackbird (Agelaius phoeniceus) | |
| Beamish et al. | Bioenergetics of the southern brook lamprey, Ichthyomyzon gagei | |
| Larsen et al. | Semen extenders for artificial insemination in the American alligator | |
| Baker et al. | Muskox (Ovibos moschatus) milk. I. Gross composition, fatty acid, and mineral constitution | |
| Ohta et al. | Induction of sperm maturation | |
| Vibe | The musk ox in East Greenland | |
| Glogowski et al. | Factors affecting cryopreservation efficiency and enzyme activity in northern pike, Esox lucius, sperm | |
| SU604556A1 (en) | Synthetic medium for diluting and freezing animal sperm | |
| Buckland | The Activity of Six Enzymes of Chicken Seminal Plasma and Sperm: 2. The Relationship between Enzyme Activity and Fertility of Fresh and Stored Semen | |
| Glogowski et al. | Properties and cryopreservation of Danube salmon (Hucho hucho) milt | |
| SU399237A1 (en) | MEDIUM FOR DILUTION OF SPERM | |
| SU626773A1 (en) | Composition for diluting and freezing sperm of agricultural animals | |
| SU1138149A1 (en) | Medium for diluting and freezing sperm of farm animals | |
| Rees et al. | An inhibitor of salt absorption in root tissue of red beet | |
| JP2013078272A (en) | Cow semen preservation liquid |