SU1427252A1 - Device for measuring luminescence - Google Patents

Description

and passes the light flux with the excitation wavelength, the filter 7 reflects the light flux with the excitation wavelength, and passes the light flux with the luminescence wavelength. At. In this, the ellipses are as close as possible to the cell with the test substance, and therefore the diaphragm of the luminescence flux of the object detected by the photodetector is determined only by geometrical parameters.

standardized cell, not optical registration. The sample introduction system allows for the analysis of a wide class of substances, to investigate reactions of various types and influence the course of reactions. By using a mirror-reflecting system that maximizes the solid angle at which the luminous flux is measured, the sensitivity of measurements is increased.

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The invention relates to analytical instrumentation and is intended to determine the composition and quality of liquid media, including non-opacification, as well as to make measurements on synthetic polymer membranes by luminescent methods.

The purpose of the invention is to increase the sensitivity of measurements and improve the manufacturability of the design.

The drawing shows a block diagram of a device for measuring luminescence -. of

; The device contains a source of light 1, a re-reflective system 2 comprising lenses between which a light filter 3 is located and truncated mirror ellipses 4. The light flux from the light source is redistributed by the upper ellipse to the sample 5, causing the fluorescence of the substance, which the ellipse pererasp {) goes to the photodetector 6 with a light filter 7. The sample reagent injection device allows you to enter reagents through the capillaries (three-channel) of the sample introduction system 8 directly at the time of measurement, which means It expands the analytical capabilities of the device. The microprocessor device 9 allows controlling the movement of the plate 10, the dosing of reagents and, by sampling, the luminescence information received from the photodetector 6.

To increase the technological effectiveness of the design, the mirror-reflecting system is made of two truncated ellipses. Filter 3 reflects the luminous flux with the luminescence wavelength, and transmits the luminous flux with a length of about the excitation wave, and filter 7 reflects the luminous flux with the excitation wavelength, and transmits the luminous flux with the luminescence wavelength. At the same time, the ellipses are maximally close to the cell with the test substance, and therefore the diaphragm of the luminescence of the object detected by the photoreceiver will be determined only by geometrical

5 parameters of a standardized cell, and not an optical registration scheme. The sample entry system allows for the analysis of a wide class of all kinds of reactions to investigate different types of reactions and influence the course of reactions.

The device operates as follows.

The light flux from the source is formed by a re-reflective system 2 in the first focus of the upper ellipse and is focused on the sample 5, which is located in one of the foci of the lower ellipse, so that the luminescence flux of the sample is redistributed by the lower ellipse to the sensitive area of the photodetector 6. This results in an effective luminescence collection and maximum use of the luminous flux of the radiation source5. By using a mirror-reflecting system that maximizes the solid angle at which the luminous flux is measured, the sensitivity of measurements is increased.

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Claims (1)

A device for measuring luminescence, comprising an optically coupled light source, a re-reflecting system of lenses, between which light filters are located, with a light source, a specularly reflecting system for collecting rays, and a light detector with a light filter located in the front focus of the re-reflecting system The output of which is connected to the input of the microprocessor control and information processing device, and the outputs of the latter are connected to a light source} 5 and the input system scanning device samples that differ in temr that, in order to increase the sensitivity of measurements and improve the manufacturability of the construction, the specular reflectance system is designed as two consecutive truncated ellipses with conjugate foci located between the cell with the sample under investigation and the photoreceiver with a light filter directly above the cell under investigation the capillaries, which are equipped with the sample introduction system, at the junction point of the ellipses are located a cell with the sample under investigation, the second photo the upper ellipse cusp is conjugated — with a back focus re-echoed by the circuit of the system, and a photodetector is located in the second focus of the lower ellipse.