SG11202102106RA - Cellulose-synthase-like enzymes and uses thereof - Google Patents

Cellulose-synthase-like enzymes and uses thereof

Info

Publication number
SG11202102106RA
SG11202102106RA SG11202102106RA SG11202102106RA SG11202102106RA SG 11202102106R A SG11202102106R A SG 11202102106RA SG 11202102106R A SG11202102106R A SG 11202102106RA SG 11202102106R A SG11202102106R A SG 11202102106RA SG 11202102106R A SG11202102106R A SG 11202102106RA
Authority
SG
Singapore
Prior art keywords
synthase
enzymes
cellulose
Prior art date
Application number
SG11202102106RA
Inventor
Asaph Aharoni
Prashant Sonawane
Maxim Itkin
Adam JOZWIAK
Original Assignee
Yeda Res & Dev
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US16/123,248 external-priority patent/US10806119B2/en
Priority claimed from IL268269A external-priority patent/IL268269A/en
Application filed by Yeda Res & Dev filed Critical Yeda Res & Dev
Publication of SG11202102106RA publication Critical patent/SG11202102106RA/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/82—Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1059—Cellulose synthases (2.4.1.12; 2.4.1.29)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01017—Glucuronosyltransferase (2.4.1.17)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Virology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
SG11202102106RA 2018-09-06 2019-09-05 Cellulose-synthase-like enzymes and uses thereof SG11202102106RA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16/123,248 US10806119B2 (en) 2013-06-05 2018-09-06 Plant with altered content of steroidal alkaloids
IL268269A IL268269A (en) 2019-07-25 2019-07-25 Production of high-value saponins by the use of cellulose-synthase-like family enzymes
PCT/IL2019/051000 WO2020049572A1 (en) 2018-09-06 2019-09-05 Cellulose-synthase-like enzymes and uses thereof

Publications (1)

Publication Number Publication Date
SG11202102106RA true SG11202102106RA (en) 2021-04-29

Family

ID=69721605

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202102106RA SG11202102106RA (en) 2018-09-06 2019-09-05 Cellulose-synthase-like enzymes and uses thereof

Country Status (8)

Country Link
US (1) US20220217934A1 (en)
EP (1) EP3847250A1 (en)
JP (1) JP2022500023A (en)
CN (1) CN112969785A (en)
CA (1) CA3111386A1 (en)
IL (1) IL281171A (en)
SG (1) SG11202102106RA (en)
WO (1) WO2020049572A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201909104D0 (en) 2019-06-25 2019-08-07 Plant Bioscience Ltd Transferase enzymes
JPWO2021075572A1 (en) * 2019-10-17 2021-04-22
CN113430194B (en) * 2020-11-30 2023-04-07 东北林业大学 White birch gene editing method based on CRISPR/Cas9
WO2023212651A2 (en) * 2022-04-27 2023-11-02 The Regents Of The University Of Colorado A Body Corporate Enhanced production of squalene in plant systems
GB202209501D0 (en) * 2022-06-29 2022-08-10 Plant Bioscience Ltd Biosynthetic enzymes
GB202209588D0 (en) * 2022-06-29 2022-08-10 Plant Bioscience Ltd Methods and compositions
WO2024047057A1 (en) * 2022-09-01 2024-03-07 Vib Vzw Means and methods to produce triterpene saponins in eukaryotic cells

Family Cites Families (28)

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US4987071A (en) 1986-12-03 1991-01-22 University Patents, Inc. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods
US5807718A (en) 1994-12-02 1998-09-15 The Scripps Research Institute Enzymatic DNA molecules
US5959180A (en) 1997-02-07 1999-09-28 The United States Of America As Represented By The Secretary Of Agriculture DNA sequences from potato encoding solanidine UDP-glucose glucosyltransferase and use to reduce glycoalkaloids in solanaceous plants
JP2002534054A (en) 1997-09-05 2002-10-15 ペンタファルム アクチェンゲゼルシャフト Expression vectors for producing improved yeast polypeptides
DE19919550A1 (en) 1999-04-29 2000-11-02 Norddeutsche Pflanzenzucht Han Process for the production of organisms with increased sterol glycoside production and use of the sterol glycosides
US6562595B2 (en) 2000-02-18 2003-05-13 Mcgill University Dominant selectable marker for gene transformation and disruption in yeasts
US20120094385A1 (en) 2000-07-31 2012-04-19 Kuehnle Agrosystems, Inc. Gene positioning system for plastidic transformation and products thereof
KR100430785B1 (en) 2001-06-21 2004-05-10 송재만 Yeast transforming vector containing dominant nutrient-requiring gene, yeast transformant containing it and preparing process thereof
DK1554387T3 (en) 2002-10-15 2012-05-14 Syngenta Participations Ag plastid
US7375259B1 (en) 2005-11-14 2008-05-20 The United States Of America, As Represented By The Secretary Of Agriculture Solanum tuberosum sterol alkaloid glycosyltransferase (SGT) a novel solanidine glucosyltransferase SGT2 and uses thereof
US7439419B1 (en) 2005-11-14 2008-10-21 The United States Of America As Represented By The Secretary Of Agriculture Solanum tuberosum β-solanine/β-chaconine rhamnosyl transferase sequences and uses thereof
JP4737531B2 (en) 2005-12-05 2011-08-03 サントリーホールディングス株式会社 Method for producing ceramide using transformed yeast
EP1854878A1 (en) 2006-05-10 2007-11-14 Cosmoferm B.V. Methods and materials for the transformation of the yeast Pichia ciferrii
JP2009034036A (en) 2007-08-01 2009-02-19 Toyota Motor Corp Yeast for transformation, transformation method, and method of producing substance
MX2010009679A (en) 2008-03-03 2010-09-22 Abbott Lab Methods for transforming yeast.
CA2735965A1 (en) * 2008-09-04 2010-03-11 Suntory Holdings Limited Glucuronyltransferase and polynucleotide encoding the same
EP2206723A1 (en) 2009-01-12 2010-07-14 Bonas, Ulla Modular DNA-binding domains
EP2383337B1 (en) 2009-01-23 2015-03-04 Kochi University Novel promoter for use in transformation of algae
KR20100095125A (en) 2009-02-20 2010-08-30 주식회사 포비커 Advertisement system based on location
US20120034698A1 (en) 2009-04-02 2012-02-09 Rosetta Green Ltd. Compositions and methods for increasing oil content in algae
EP2471927B1 (en) 2009-08-28 2015-12-23 Kirin Holdings Kabushiki Kaisha Protein having glycoalkaloid biosynthase activity, and gene encoding same
CN102753687B (en) 2010-02-17 2014-05-07 国立大学法人高知大学 Novel promoter for use in transformation of algae
JP5794770B2 (en) 2010-08-31 2015-10-14 キリンホールディングス株式会社 Protein having glycoalkaloid biosynthetic enzyme activity and gene encoding the same
WO2012095843A1 (en) * 2011-01-13 2012-07-19 Yeda Research And Development Co. Ltd. Organisms with altered steroidal saponin and steroidal alkaloid levels and methods for producing same
US20160046912A1 (en) * 2013-04-04 2016-02-18 Riken Glucuronosyltransferase, gene encoding same and use thereof
US10100322B2 (en) * 2013-06-05 2018-10-16 Yeda Research And Development Co. Ltd. Plant with altered content of steroidal glycoalkaloids
US10806119B2 (en) * 2013-06-05 2020-10-20 Yeda Research And Development Co. Ltd. Plant with altered content of steroidal alkaloids
JP5721191B2 (en) 2013-07-02 2015-05-20 国立大学法人京都大学 Gene transfer method to algal cells using three-stage rectangular wave multiple pulses

Also Published As

Publication number Publication date
CA3111386A1 (en) 2020-03-12
JP2022500023A (en) 2022-01-04
EP3847250A1 (en) 2021-07-14
CN112969785A (en) 2021-06-15
US20220217934A1 (en) 2022-07-14
IL281171A (en) 2021-04-29
WO2020049572A1 (en) 2020-03-12

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