SG11202011180UA - Automated detection and characterization of micro-objects in microfluidic devices - Google Patents

Automated detection and characterization of micro-objects in microfluidic devices

Info

Publication number
SG11202011180UA
SG11202011180UA SG11202011180UA SG11202011180UA SG11202011180UA SG 11202011180U A SG11202011180U A SG 11202011180UA SG 11202011180U A SG11202011180U A SG 11202011180UA SG 11202011180U A SG11202011180U A SG 11202011180UA SG 11202011180U A SG11202011180U A SG 11202011180UA
Authority
SG
Singapore
Prior art keywords
characterization
micro
objects
microfluidic devices
automated detection
Prior art date
Application number
SG11202011180UA
Inventor
John A Tenney
Thomas M Vetterli
Hansohl E Kim
Original Assignee
Berkeley Lights Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Berkeley Lights Inc filed Critical Berkeley Lights Inc
Publication of SG11202011180UA publication Critical patent/SG11202011180UA/en

Links

Classifications

    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/11—Region-based segmentation
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/174—Segmentation; Edge detection involving the use of two or more images
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/30—Determination of transform parameters for the alignment of images, i.e. image registration
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10064—Fluorescence image
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10141—Special mode during image acquisition
    • G06T2207/10152—Varying illumination
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20081—Training; Learning
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20084—Artificial neural networks [ANN]
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20212—Image combination
    • G06T2207/20224—Image subtraction
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30242—Counting objects in image

Landscapes

  • Engineering & Computer Science (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Theoretical Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Medical Informatics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Radiology & Medical Imaging (AREA)
  • Quality & Reliability (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
SG11202011180UA 2018-05-31 2019-05-31 Automated detection and characterization of micro-objects in microfluidic devices SG11202011180UA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862678791P 2018-05-31 2018-05-31
PCT/US2019/035046 WO2019232473A2 (en) 2018-05-31 2019-05-31 Automated detection and characterization of micro-objects in microfluidic devices

Publications (1)

Publication Number Publication Date
SG11202011180UA true SG11202011180UA (en) 2020-12-30

Family

ID=68698993

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202011180UA SG11202011180UA (en) 2018-05-31 2019-05-31 Automated detection and characterization of micro-objects in microfluidic devices

Country Status (10)

Country Link
US (1) US20210209752A1 (en)
EP (1) EP3803341A4 (en)
JP (1) JP2021525359A (en)
KR (1) KR20210015947A (en)
CN (1) CN112204380A (en)
AU (1) AU2019276567A1 (en)
CA (1) CA3100701A1 (en)
IL (1) IL279058A (en)
SG (1) SG11202011180UA (en)
WO (1) WO2019232473A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10675625B2 (en) 2016-04-15 2020-06-09 Berkeley Lights, Inc Light sequencing and patterns for dielectrophoretic transport
JP2019537157A (en) 2016-12-01 2019-12-19 バークレー ライツ，インコーポレイテッド Automatic detection and relocation of minute objects by microfluidic devices
SG10202104487WA (en) 2016-12-01 2021-05-28 Berkeley Lights Inc Apparatuses, systems and methods for imaging micro-objects
WO2018111765A1 (en) 2016-12-12 2018-06-21 xCella Biosciences, Inc. Methods and systems for screening using microcapillary arrays
US11450009B2 (en) * 2018-02-26 2022-09-20 Intel Corporation Object detection with modified image background
JP6954473B2 (en) * 2018-07-23 2021-10-27 株式会社島津製作所 Microfluidic device observation device
AU2020361681A1 (en) 2019-10-10 2022-05-05 1859, Inc. Methods and systems for microfluidic screening
US11335636B2 (en) * 2019-10-29 2022-05-17 Hefei Reliance Memory Limited Gradual breakdown memory cell having multiple different dielectrics
TW202142856A (en) 2019-11-17 2021-11-16 美商伯克利之光生命科技公司 Systems and methods for analyses of biological samples
CN115004226A (en) 2020-02-21 2022-09-02 索高视觉有限公司 Medical procedure assisted with luminescent images processed in limited information areas identified in corresponding auxiliary images
CN112907665B (en) * 2021-02-07 2022-06-10 吉林大学 Micro-fluidic blood type detection card micro-cavity reaction tank precise positioning method based on RGB color space
CN114627369B (en) * 2022-01-19 2024-03-22 上海土蜂科技有限公司 Environment monitoring system, method and computer device thereof
CN116758070B (en) * 2023-08-17 2023-12-12 深圳安侣医学科技有限公司 Method and system for 3-dimensional analysis of cells and obtaining 3-dimensional morphological characteristics of cells

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
US6942776B2 (en) 1999-05-18 2005-09-13 Silicon Biosystems S.R.L. Method and apparatus for the manipulation of particles by means of dielectrophoresis
KR100865105B1 (en) 1999-06-28 2008-10-24 캘리포니아 인스티튜트 오브 테크놀로지 Microfabricated elastomeric valve and pump systems
JP2002335400A (en) * 2001-05-07 2002-11-22 Sharp Corp Image processing unit, image processing method and recording medium
US6958132B2 (en) 2002-05-31 2005-10-25 The Regents Of The University Of California Systems and methods for optical actuation of microfluidics based on opto-electrowetting
WO2005100541A2 (en) 2004-04-12 2005-10-27 The Regents Of The University Of California Optoelectronic tweezers for microparticle and cell manipulation
US8685344B2 (en) 2007-01-22 2014-04-01 Advanced Liquid Logic, Inc. Surface assisted fluid loading and droplet dispensing
WO2008119066A1 (en) 2007-03-28 2008-10-02 The Regents Of The University Of California Single-sided lateral-field and phototransistor-based optoelectronic tweezers
CN101681515A (en) * 2007-06-21 2010-03-24 皇家飞利浦电子股份有限公司 Model-based differential diagnosis of dementia and interactive setting of level of significance
ITTO20070771A1 (en) * 2007-10-29 2009-04-30 Silicon Biosystems Spa METHOD AND APPARATUS FOR IDENTIFICATION AND HANDLING OF PARTICLES
DE212009000021U1 (en) * 2008-01-28 2010-09-23 Honeywell International Inc. Ground shield with high thread
FR2935802B1 (en) * 2008-09-05 2012-12-28 Horiba Abx Sas METHOD AND DEVICE FOR CLASSIFYING, VISUALIZING AND EXPLORING BIOLOGICAL DATA
US9025850B2 (en) * 2010-06-25 2015-05-05 Cireca Theranostics, Llc Method for analyzing biological specimens by spectral imaging
US9533306B2 (en) 2010-08-02 2017-01-03 The Regents Of The University Of California Single sided continuous optoelectrowetting (SCEOW) device for droplet manipulation with light patterns
US20120225475A1 (en) * 2010-11-16 2012-09-06 1087 Systems, Inc. Cytometry system with quantum cascade laser source, acoustic detector, and micro-fluidic cell handling system configured for inspection of individual cells
WO2012119243A2 (en) * 2011-03-07 2012-09-13 James Stewart Aitchison Method and system for cell detection and analysis
US9227200B2 (en) 2011-06-03 2016-01-05 The Regents Of The University Of California Microfluidic devices with flexible optically transparent electrodes
EP2602608B1 (en) * 2011-12-07 2016-09-14 Imec Analysis and sorting of biological cells in flow
CA2875710C (en) * 2012-06-22 2021-06-29 John Wayne Cancer Institute Molecular malignancy in melanocytic lesions
US9857333B2 (en) 2012-10-31 2018-01-02 Berkeley Lights, Inc. Pens for biological micro-objects
US9403172B2 (en) 2012-11-08 2016-08-02 Berkeley Lights, Inc. Circuit based optoelectronic tweezers
US9889445B2 (en) 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
US10010882B2 (en) * 2013-10-22 2018-07-03 Berkeley Lights, Inc. Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
US20150306599A1 (en) 2014-04-25 2015-10-29 Berkeley Lights, Inc. Providing DEP Manipulation Devices And Controllable Electrowetting Devices In The Same Microfluidic Apparatus
US20150306598A1 (en) 2014-04-25 2015-10-29 Berkeley Lights, Inc. DEP Force Control And Electrowetting Control In Different Sections Of The Same Microfluidic Apparatus
SG11201608499XA (en) 2014-04-25 2016-11-29 Berkeley Lights Inc Providing dep manipulation devices and controllable electrowetting devices in the same microfluidic apparatus
WO2016094459A2 (en) * 2014-12-09 2016-06-16 Berkeley Lights, Inc. Automated detection and repositioning of micro-objects in microfluidic devices
CN107206377B (en) * 2014-12-09 2020-02-11 伯克利之光生命科技公司 Automated detection of positive assay regions in microfluidic devices
EP3262417B1 (en) * 2015-02-23 2021-11-03 Cellanyx Diagnostics, LLC Cell imaging and analysis to differentiate clinically relevant sub-populations of cells
WO2016172623A1 (en) * 2015-04-22 2016-10-27 Berkeley Lights, Inc. Manipulation of cell nuclei in a micro-fluidic device
JP6902548B2 (en) * 2016-01-15 2021-07-14 バークレー ライツ，インコーポレイテッド Manufacturing method of patient-specific anticancer therapeutic agent and its therapeutic method
US20180135011A1 (en) * 2017-03-13 2018-05-17 Berkeley Lights, Inc. Selection and cloning of t lymphocytes in a microfluidic device
CN115354025A (en) * 2016-03-17 2022-11-18 伯克利之光生命科技公司 Selection and cloning of T lymphocytes in microfluidic devices
EP3469338A4 (en) * 2016-06-10 2020-01-15 The Regents of the University of California Image-based cell sorting systems and methods

Also Published As

Publication number Publication date
US20210209752A1 (en) 2021-07-08
CA3100701A1 (en) 2019-12-05
AU2019276567A1 (en) 2021-01-28
IL279058A (en) 2021-01-31
WO2019232473A3 (en) 2020-01-16
WO2019232473A2 (en) 2019-12-05
CN112204380A (en) 2021-01-08
KR20210015947A (en) 2021-02-10
EP3803341A4 (en) 2022-03-23
JP2021525359A (en) 2021-09-24
EP3803341A2 (en) 2021-04-14

Similar Documents

Publication Publication Date Title
IL279058A (en) Automated detection and characterization of micro-objects in microfluidic devices
IL267009A (en) Automated detection and repositioning of micro-objects in microfluidic devices
HK1244874A1 (en) Automated detection and repositioning of micro-objects in microfluidic devices
HK1244465A1 (en) Automated detection of assay-positive areas in microfluidic devices
HK1251660A1 (en) Microfluidic droplet detection and sorting
SG11201914032VA (en) Single-channel chemiluminescent micro-fluidic chip and detection method thereof
GB2564582B (en) Systems and methods for growth of intestinal cells in microfluidic devices
EP3548889A4 (en) Automated point-of-care devices for complex sample processing and methods of use thereof
HK1251659A1 (en) Droplet microfluidic device and methods of sensing the result of an assay therein
SG10202101365TA (en) Integrated microfluidic system for culturing and testing
SG11201607270PA (en) A multi-index detection microfluidic chip and methods of use
SG11201609288YA (en) Fully-automatic immunofluorescence quantitative analysis device and detection method
HK1258244A1 (en) Fluidic analysis and separation
EP3508124A4 (en) Biological measurement device and biological measurement method
EP3485973A4 (en) Microfluidic reagent card and detection method and application thereof
EP3252476A4 (en) Liquid surface inspection device, automated analysis device, and processing device
SG10201912318VA (en) Analyte detection methods and apparatus using dielectrophoresis and electroosmosis
EP3410107A4 (en) Analysis device and separation device
SG11201901202UA (en) Inspection device and inspection methods
IL252403B (en) Amplification and detection of nucleic acids in a biological sample
SG11201706790TA (en) L-fabp immunoassay method and assay reagent used in said method
EP3275549A4 (en) Microfluidic device and sample analysis device comprising same
HK1247987A1 (en) Fluidic bridge device and sample processing methods
EP3528030A4 (en) Sample observation device and sample observation method
EP3644046A4 (en) Specimen detection device and specimen detection method