SG11202000775SA - Fusion tags for recombinant protein expression - Google Patents

Fusion tags for recombinant protein expression

Info

Publication number
SG11202000775SA
SG11202000775SA SG11202000775SA SG11202000775SA SG11202000775SA SG 11202000775S A SG11202000775S A SG 11202000775SA SG 11202000775S A SG11202000775S A SG 11202000775SA SG 11202000775S A SG11202000775S A SG 11202000775SA SG 11202000775S A SG11202000775S A SG 11202000775SA
Authority
SG
Singapore
Prior art keywords
protein expression
recombinant protein
fusion tags
tags
fusion
Prior art date
Application number
SG11202000775SA
Inventor
Michael Hecht
Shlomo Zarzhitsky
Original Assignee
Univ Princeton
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Princeton filed Critical Univ Princeton
Publication of SG11202000775SA publication Critical patent/SG11202000775SA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SG11202000775SA 2017-07-28 2018-07-27 Fusion tags for recombinant protein expression SG11202000775SA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762538505P 2017-07-28 2017-07-28
PCT/US2018/044156 WO2019023616A1 (en) 2017-07-28 2018-07-27 Fusion tags for recombinant protein expression

Publications (1)

Publication Number Publication Date
SG11202000775SA true SG11202000775SA (en) 2020-02-27

Family

ID=63312460

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202000775SA SG11202000775SA (en) 2017-07-28 2018-07-27 Fusion tags for recombinant protein expression

Country Status (10)

Country Link
US (1) US20200216512A1 (en)
EP (1) EP3658574A1 (en)
JP (1) JP2020529221A (en)
KR (1) KR20200037819A (en)
CN (1) CN111132996A (en)
AU (1) AU2018307960B2 (en)
CA (1) CA3071419A1 (en)
MX (1) MX2020001058A (en)
SG (1) SG11202000775SA (en)
WO (1) WO2019023616A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022094146A1 (en) 2020-10-28 2022-05-05 The Trustees Of Princeton University Methods of converting precursor proteins to mature proteins using kex2 proteases
EP4277917A2 (en) * 2021-01-14 2023-11-22 Biocatalyst LLC Compositions and methods for peptide production
CN113388009B (en) * 2021-05-18 2022-07-19 中国科学院福建物质结构研究所 Tag protein, coding gene thereof, recombinant vector and application
KR20240043839A (en) * 2022-09-27 2024-04-04 크리포 주식회사 A fusion protein that forms self-assembly comprising a dispersed His-tag based peptide tag in which major amino acids are composed of charged and hydrophobic amino acids, and method for purifying recombinant protein using the same

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2705895A (en) 1994-06-30 1996-01-25 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
CN103031328B (en) * 2012-11-22 2015-08-05 江南大学 A kind of fusion tag is utilized to improve the expression amount of exogenous protein
AU2014241743B2 (en) 2013-03-14 2018-07-05 Indiana University Research And Technology Corporation Insulin-incretin conjugates
WO2015015518A2 (en) * 2013-07-31 2015-02-05 Biogenomics Limited Process for production of insulin and insulin analogues

Also Published As

Publication number Publication date
JP2020529221A (en) 2020-10-08
AU2018307960A1 (en) 2020-02-27
CA3071419A1 (en) 2019-01-31
KR20200037819A (en) 2020-04-09
MX2020001058A (en) 2020-08-06
CN111132996A (en) 2020-05-08
AU2018307960B2 (en) 2023-03-23
WO2019023616A1 (en) 2019-01-31
US20200216512A1 (en) 2020-07-09
EP3658574A1 (en) 2020-06-03

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