SG11201608993RA - Detection of dna that originates from a specific cell-type and related methods - Google Patents

Detection of dna that originates from a specific cell-type and related methods

Info

Publication number
SG11201608993RA
SG11201608993RA SG11201608993RA SG11201608993RA SG11201608993RA SG 11201608993R A SG11201608993R A SG 11201608993RA SG 11201608993R A SG11201608993R A SG 11201608993RA SG 11201608993R A SG11201608993R A SG 11201608993RA SG 11201608993R A SG11201608993R A SG 11201608993RA
Authority
SG
Singapore
Prior art keywords
originates
dna
detection
type
specific cell
Prior art date
Application number
SG11201608993RA
Inventor
Sebastian Grömminger
Wera Hofmann
Hamed Said
Matthias Sachse
Original Assignee
Lifecodexx Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP14167769.0A external-priority patent/EP2942400A1/en
Priority claimed from EP14167775.7A external-priority patent/EP2942401A1/en
Application filed by Lifecodexx Ag filed Critical Lifecodexx Ag
Publication of SG11201608993RA publication Critical patent/SG11201608993RA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/154Methylation markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
SG11201608993RA 2014-05-09 2015-05-08 Detection of dna that originates from a specific cell-type and related methods SG11201608993RA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP14167769.0A EP2942400A1 (en) 2014-05-09 2014-05-09 Multiplex detection of DNA that originates from a specific cell-type
EP14167775.7A EP2942401A1 (en) 2014-05-09 2014-05-09 Detection of DNA that originates from a specific cell-type
PCT/EP2015/060188 WO2015169947A1 (en) 2014-05-09 2015-05-08 Detection of dna that originates from a specific cell-type and related methods

Publications (1)

Publication Number Publication Date
SG11201608993RA true SG11201608993RA (en) 2016-11-29

Family

ID=53189029

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201608993RA SG11201608993RA (en) 2014-05-09 2015-05-08 Detection of dna that originates from a specific cell-type and related methods

Country Status (11)

Country Link
US (2) US10801067B2 (en)
EP (2) EP3140421B8 (en)
JP (1) JP6681841B2 (en)
CN (1) CN107223159A (en)
AU (1) AU2015257654A1 (en)
CA (1) CA2985134A1 (en)
IL (1) IL248637A0 (en)
MA (1) MA39951A (en)
RU (1) RU2016147914A (en)
SG (1) SG11201608993RA (en)
WO (1) WO2015169947A1 (en)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
KR101912401B1 (en) 2012-02-03 2018-10-26 캘리포니아 인스티튜트 오브 테크놀로지 Signal encoding and decoding in multiplexed biochemical assays
WO2014022827A1 (en) 2012-08-03 2014-02-06 California Institute Of Technology Multiplexing and quantification in pcr with reduced hardware and requirements
DK3643795T3 (en) 2014-04-14 2022-05-09 Yissum Research And Development Company Of The Hebrew Univ Of Jerusalem Ltd METHOD FOR DETERMINING CELL OR TISSUE DEATH IN ANALYSIS OF DNA METHYLATION
EP2942400A1 (en) * 2014-05-09 2015-11-11 Lifecodexx AG Multiplex detection of DNA that originates from a specific cell-type
TWI813141B (en) 2014-07-18 2023-08-21 香港中文大學 Methylation pattern analysis of tissues in a dna mixture
EP3262191B2 (en) 2015-02-24 2023-06-14 Zymo Research Corporation Assays to determine dna methylation and dna methylation markers of cancer
WO2016183106A1 (en) 2015-05-11 2016-11-17 Natera, Inc. Methods and compositions for determining ploidy
SG10202107693TA (en) * 2015-09-22 2021-09-29 Univ Hong Kong Chinese Accurate quantification of fetal dna fraction by shallow-depth sequencing of maternal plasma dna
JP6979554B2 (en) * 2016-05-03 2021-12-15 ユニバーシティー ヘルス ネットワーク Methods and Uses for Capturing Cell-Free Methylated DNA
CA3025708A1 (en) 2016-05-30 2017-12-07 The Chinese University Of Hong Kong Detecting hematological disorders using cell-free dna in blood
AU2017347790A1 (en) 2016-10-24 2019-05-23 Grail, Inc. Methods and systems for tumor detection
JP2019534016A (en) * 2016-11-02 2019-11-28 ザ メディカル カレッジ オブ ウィスコンシン,インコーポレイテッドThe Medical College of Wisconsin, Inc. Method for assessing risk using total and specific cell-free DNA
CA3039685A1 (en) * 2016-11-30 2018-06-07 The Chinese University Of Hong Kong Analysis of cell-free dna in urine and other samples
SG11201906397UA (en) 2017-01-25 2019-08-27 Univ Hong Kong Chinese Diagnostic applications using nucleic acid fragments
WO2018237075A1 (en) 2017-06-20 2018-12-27 The Medical College Of Wisconsin, Inc. Assessing transplant complication risk with total cell-free dna
EP3652344A1 (en) 2017-07-13 2020-05-20 Yissum Research and Development Company of the Hebrew University of Jerusalem Ltd. Dna targets as tissue-specific methylation markers
WO2019012544A1 (en) 2017-07-13 2019-01-17 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Dual-probe digital droplet pcr strategy for specific detection of tissue-specific circulating dna molecules
WO2019012542A1 (en) 2017-07-13 2019-01-17 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Detecting tissue-specific dna
EP3766076A4 (en) * 2018-03-15 2021-12-29 Grail, Inc. Tissue-specific methylation marker
WO2019204357A1 (en) * 2018-04-17 2019-10-24 ChromaCode, Inc. Methods and systems for multiplex analysis
WO2019236478A1 (en) * 2018-06-04 2019-12-12 Guardant Health, Inc. Methods and systems for determining the cellular origin of cell-free nucleic acids
CN109385464A (en) * 2018-07-27 2019-02-26 中山大学附属第六医院 A kind of DNA methylation detection kit and method
CN109706242A (en) * 2019-01-31 2019-05-03 佛山市顺德区辉锦创兴生物医学科技有限公司 Myocardial cell damage detection kit and its application
US11931674B2 (en) 2019-04-04 2024-03-19 Natera, Inc. Materials and methods for processing blood samples
EP3987056A4 (en) * 2019-06-24 2023-07-26 Chromacode, Inc. Methods for differentially quantifying nucleic acids
CN112176419B (en) * 2019-10-16 2022-03-22 中国医学科学院肿瘤医院 Method for detecting variation and methylation of tumor specific genes in ctDNA
CA3154354A1 (en) * 2019-10-31 2021-05-06 William R. Taylor Detecting ovarian cancer
CN113249458B (en) * 2020-02-10 2023-07-07 北京优乐复生科技有限责任公司 Method and kit for predicting evaluation and prediction of placenta-derived diseases
CN112662761A (en) * 2020-03-05 2021-04-16 博尔诚(北京)科技有限公司 Probe composition for detecting 3 parenchymal organ tumors
CA3176037A1 (en) * 2020-05-18 2021-11-25 Brian BIXON Handling and tracking of biological specimens for cryogenic storage
CN111763725A (en) * 2020-07-02 2020-10-13 济南国科医工科技发展有限公司 Kit for 21-trisomy risk detection
RU2738680C1 (en) * 2020-08-07 2020-12-15 Федеральное государственное автономное образовательное учреждение высшего образования "Белгородский государственный национальный исследовательский университет" (НИУ "БелГУ") Method for prediction of the risk of developing fetal growth retardation syndrome in women without pre-existing family history using molecular genetic data
WO2023133572A2 (en) * 2022-01-10 2023-07-13 Washington State University Dna methylation biomarkers for preterm birth
WO2023227954A1 (en) * 2022-05-22 2023-11-30 Nucleix Ltd. Sample preparation for cell-free dna analysis
US20240084374A1 (en) * 2022-09-13 2024-03-14 Bio-Rad Laboratories, Inc. Method for estimation of fetal fraction in cell-free dna from maternal sample

Family Cites Families (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
ATE198511T1 (en) 1994-04-29 2001-01-15 Perkin Elmer Corp METHOD AND DEVICE FOR REAL-TIME DETECTION OF NUCLEIC ACID AMPLIFICATION PRODUCTS
US6017704A (en) 1996-06-03 2000-01-25 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US5786146A (en) 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
DE19906169A1 (en) 1999-02-08 2000-08-10 Bioinside Ges Fuer Biodiagnost Test kit and method for the quantitative detection of genetically modified DNA in food using fluorescence-coupled PCR
US6331393B1 (en) 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
US6727356B1 (en) 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
US6929907B2 (en) 1999-12-31 2005-08-16 North Carolina State University Methods and compositions for determining the purity of chemically synthesized nucleic acids
US6927028B2 (en) 2001-08-31 2005-08-09 Chinese University Of Hong Kong Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA
US20030165859A1 (en) 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
US20030211522A1 (en) 2002-01-18 2003-11-13 Landes Gregory M. Methods for fetal DNA detection and allele quantitation
US20040229211A1 (en) 2003-05-13 2004-11-18 Yeung Wah Hin Alex Sensitive diagnostic testing methodology using multiplex real time PCR with one dye (MOD) and its use as in severe acute respiratory syndrome (SARS)
WO2005035725A2 (en) 2003-10-08 2005-04-21 The Trustees Of Boston University Methods for prenatal diagnosis of chromosomal abnormalities
DE60328193D1 (en) 2003-10-16 2009-08-13 Sequenom Inc Non-invasive detection of fetal genetic traits
WO2005042713A2 (en) 2003-10-28 2005-05-12 The Johns Hopkins University Quantitative multiplex methylation-specific pcr
JP2005261354A (en) * 2004-03-19 2005-09-29 Arkray Inc Fluorescence detection method for nucleic acid
CA2562390C (en) 2004-04-07 2014-12-02 Exiqon A/S Novel methods for quantification of micrornas and small interfering rnas
US7709194B2 (en) 2004-06-04 2010-05-04 The Chinese University Of Hong Kong Marker for prenatal diagnosis and monitoring
US20070059753A1 (en) 2005-09-15 2007-03-15 Tatiana Vener Detecting gene methylation
US7901884B2 (en) 2006-05-03 2011-03-08 The Chinese University Of Hong Kong Markers for prenatal diagnosis and monitoring
US7754428B2 (en) 2006-05-03 2010-07-13 The Chinese University Of Hong Kong Fetal methylation markers
EP3617321A3 (en) 2006-05-31 2020-04-29 Sequenom, Inc. Methods and compositions for the extraction and amplification of nucleic acid from a sample
US9290803B2 (en) * 2007-04-12 2016-03-22 University Of Southern California DNA methylation analysis by digital bisulfite genomic sequencing and digital methylight
CN102216456A (en) * 2008-09-16 2011-10-12 塞昆纳姆股份有限公司 Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
US8476013B2 (en) * 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US20120252015A1 (en) 2011-02-18 2012-10-04 Bio-Rad Laboratories Methods and compositions for detecting genetic material
US8563242B2 (en) 2009-08-11 2013-10-22 The Chinese University Of Hong Kong Method for detecting chromosomal aneuploidy
EA023565B1 (en) 2010-01-26 2016-06-30 ЭнАйПиДи ДЖЕНЕТИКС ЛТД. Methods and compositions for noninvasive prenatal diagnosis of fetal aneuploidies
WO2012007783A1 (en) 2010-07-13 2012-01-19 Institut Gustave Roussy Kits and methods for detecting the ability to induce an immunogenic cancer cell death in a subject
US8603742B2 (en) 2010-07-06 2013-12-10 University of Pittsburgh—of the Commonwealth System of Higher Education Methods for the diagnosis of fetal disease
WO2012012693A2 (en) * 2010-07-23 2012-01-26 President And Fellows Of Harvard College Methods for detecting signatures of disease or conditions in bodily fluids
WO2012012703A2 (en) * 2010-07-23 2012-01-26 Esoterix Genetic Laboratories, Llc Identification of differentially represented fetal or maternal genomic regions and uses thereof
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
WO2012092599A1 (en) 2010-12-30 2012-07-05 Agco Corporation Real-time evaluation of machine performance for fleet management
US8460872B2 (en) 2011-04-29 2013-06-11 Sequenom, Inc. Quantification of a minority nucleic acid species
US10196681B2 (en) 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP2768978B1 (en) * 2011-10-18 2017-11-22 Multiplicom NV Fetal chromosomal aneuploidy diagnosis
US9892230B2 (en) 2012-03-08 2018-02-13 The Chinese University Of Hong Kong Size-based analysis of fetal or tumor DNA fraction in plasma
US20140093873A1 (en) 2012-07-13 2014-04-03 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
EP4335929A3 (en) 2012-09-20 2024-05-29 The Chinese University of Hong Kong Non-invasive determination of methylome of tumor from plasma
EP4009329A1 (en) 2012-10-04 2022-06-08 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP3597774A1 (en) 2013-03-13 2020-01-22 Sequenom, Inc. Primers for dna methylation analysis
CN105555965B (en) 2013-07-30 2020-06-05 深圳华大基因股份有限公司 Method for determining the composition of nucleic acids in a mixture of nucleic acids
WO2015138774A1 (en) 2014-03-13 2015-09-17 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP2942400A1 (en) * 2014-05-09 2015-11-11 Lifecodexx AG Multiplex detection of DNA that originates from a specific cell-type
EP2942401A1 (en) * 2014-05-09 2015-11-11 Lifecodexx AG Detection of DNA that originates from a specific cell-type
EP3283647B1 (en) 2016-06-23 2018-11-07 Trisomytest, s.r.o. A method for non-invasive prenatal detection of fetal chromosome aneuploidy from maternal blood

Also Published As

Publication number Publication date
US10801067B2 (en) 2020-10-13
AU2015257654A1 (en) 2016-11-10
CN107223159A (en) 2017-09-29
IL248637A0 (en) 2017-01-31
RU2016147914A (en) 2018-06-18
EP3521454A1 (en) 2019-08-07
EP3140421B1 (en) 2020-03-25
JP2017514499A (en) 2017-06-08
US20170314073A1 (en) 2017-11-02
WO2015169947A1 (en) 2015-11-12
WO2015169947A9 (en) 2015-12-30
EP3140421A1 (en) 2017-03-15
RU2016147914A3 (en) 2018-12-27
EP3140421B8 (en) 2020-05-06
JP6681841B2 (en) 2020-04-15
MA39951A (en) 2017-03-15
CA2985134A1 (en) 2015-11-12
US20200407793A1 (en) 2020-12-31
US11965207B2 (en) 2024-04-23

Similar Documents

Publication Publication Date Title
IL248637A0 (en) Detection of dna that originates from a specific cell-type and related methods
GB2534654B (en) Detecting and removing spoofing signals
HK1258244A1 (en) Fluidic analysis and separation
GB201407770D0 (en) Sensing methods and apparatus
GB2533284B (en) Performing object detection
IL278117A (en) Methods for tagging dna-encoded libraries
GB201408100D0 (en) Detection method
HK1243489A1 (en) Methods and systems for detection of analytes
GB201401010D0 (en) Improved separation apparatus and method
GB201401626D0 (en) Audio signal analysis
GB201409766D0 (en) Signal processing methods
GB201415761D0 (en) Methods for detection of Nucleotide modification
GB201418499D0 (en) Malware detection method
IL249990A0 (en) Improved signal detection and characterization
GB201410283D0 (en) Sensing methods and apparatus
GB201718352D0 (en) Devices and methods for detection of biomarkers
SG10201502676TA (en) Unauthorized-communication detecting apparatus and program
GB201419330D0 (en) Detection method
IL247270B (en) Method of detecting cancer
GB2537569B (en) Location detecting system and location detecting method of the location detecting system
GB201410300D0 (en) Sensing methods and apparatus
GB2541559B (en) Assessment and control of centrifuge operation
GB201416459D0 (en) Detection method
GB201405003D0 (en) Enzyme detection method
GB201402174D0 (en) Detection method