SG11201500313YA - Single cell analysis using sequence tags - Google Patents

Single cell analysis using sequence tags

Info

Publication number
SG11201500313YA
SG11201500313YA SG11201500313YA SG11201500313YA SG11201500313YA SG 11201500313Y A SG11201500313Y A SG 11201500313YA SG 11201500313Y A SG11201500313Y A SG 11201500313YA SG 11201500313Y A SG11201500313Y A SG 11201500313YA SG 11201500313Y A SG11201500313Y A SG 11201500313YA
Authority
SG
Singapore
Prior art keywords
single cell
sequence tags
cell analysis
tags
analysis
Prior art date
Application number
SG11201500313YA
Inventor
Malek Faham
Thomas Willis
Jianbiao Zheng
Original Assignee
Sequenta Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sequenta Inc filed Critical Sequenta Inc
Publication of SG11201500313YA publication Critical patent/SG11201500313YA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG11201500313YA 2012-07-24 2013-07-22 Single cell analysis using sequence tags SG11201500313YA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261675254P 2012-07-24 2012-07-24
PCT/US2013/051539 WO2014018460A1 (en) 2012-07-24 2013-07-22 Single cell analysis using sequence tags

Publications (1)

Publication Number Publication Date
SG11201500313YA true SG11201500313YA (en) 2015-02-27

Family

ID=49997760

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201500313YA SG11201500313YA (en) 2012-07-24 2013-07-22 Single cell analysis using sequence tags

Country Status (8)

Country Link
US (1) US20150247182A1 (en)
EP (1) EP2877604A1 (en)
JP (1) JP2015523087A (en)
CN (1) CN104540964A (en)
AU (1) AU2013293240A1 (en)
CA (1) CA2878694A1 (en)
SG (1) SG11201500313YA (en)
WO (1) WO2014018460A1 (en)

Families Citing this family (99)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
US9394567B2 (en) 2008-11-07 2016-07-19 Adaptive Biotechnologies Corporation Detection and quantification of sample contamination in immune repertoire analysis
EP2719774B8 (en) 2008-11-07 2020-04-22 Adaptive Biotechnologies Corporation Methods of monitoring conditions by sequence analysis
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
DK3059337T3 (en) 2009-01-15 2019-07-22 Adaptive Biotechnologies Corp Adaptive immunity profiling and methods for producing monoclonal antibodies
SG10201403451QA (en) 2009-06-25 2014-09-26 Hutchinson Fred Cancer Res Method of measuring adaptive immunity
US9043160B1 (en) 2009-11-09 2015-05-26 Sequenta, Inc. Method of determining clonotypes and clonotype profiles
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
EP2768982A4 (en) 2011-10-21 2015-06-03 Adaptive Biotechnologies Corp Quantification of adaptive immune cell genomes in a complex mixture of cells
EP3904536A1 (en) 2011-12-09 2021-11-03 Adaptive Biotechnologies Corporation Diagnosis of lymphoid malignancies and minimal residual disease detection
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
CN104364392B (en) 2012-02-27 2018-05-25 赛卢拉研究公司 For the composition and kit of numerator counts
WO2013130512A2 (en) 2012-02-27 2013-09-06 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
EP3372694A1 (en) 2012-03-05 2018-09-12 Adaptive Biotechnologies Corporation Determining paired immune receptor chains from frequency matched subunits
DK2831276T3 (en) 2012-05-08 2016-08-01 Adaptive Biotechnologies Corp Compositions and Methods for Measuring and Calibrating Amplification Bias in Multiplex PCR Reactions
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
IN2015DN01126A (en) 2012-08-14 2015-06-26 10X Genomics Inc
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2904111B1 (en) 2012-10-01 2017-12-06 Adaptive Biotechnologies Corporation Immunocompetence assessment by adaptive immune receptor diversity and clonality characterization
WO2015160439A2 (en) 2014-04-17 2015-10-22 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
DK2954065T3 (en) 2013-02-08 2021-09-06 10X Genomics Inc Division and processing of analytes and other species
CA2915499A1 (en) 2013-06-27 2014-12-31 10X Genomics, Inc. Compositions and methods for sample processing
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
KR20230074639A (en) * 2013-08-28 2023-05-30 벡톤 디킨슨 앤드 컴퍼니 Massively parallel single cell analysis
CN105745528A (en) 2013-10-07 2016-07-06 赛卢拉研究公司 Methods and systems for digitally counting features on arrays
ES2741740T3 (en) 2014-03-05 2020-02-12 Adaptive Biotechnologies Corp Methods that use synthetic molecules that contain random nucleotide segments
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
US11390921B2 (en) 2014-04-01 2022-07-19 Adaptive Biotechnologies Corporation Determining WT-1 specific T cells and WT-1 specific T cell receptors (TCRs)
CN106661620B (en) 2014-05-06 2021-11-23 贝勒医学院 Single cell whole genome linear amplification method
KR20230070325A (en) 2014-06-26 2023-05-22 10엑스 제노믹스, 인크. Methods of analyzing nucleic acids from individual cells or cell populations
EP3212790B1 (en) 2014-10-29 2020-03-25 Adaptive Biotechnologies Corp. Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from many samples
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
US11066705B2 (en) 2014-11-25 2021-07-20 Adaptive Biotechnologies Corporation Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
BR112017014902A2 (en) 2015-01-12 2018-03-13 10X Genomics Inc processes and systems for the preparation of nucleic acid sequencing libraries and libraries prepared using them
EP3259371B1 (en) 2015-02-19 2020-09-02 Becton, Dickinson and Company High-throughput single-cell analysis combining proteomic and genomic information
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
EP3262196B1 (en) 2015-02-24 2020-12-09 Adaptive Biotechnologies Corp. Method for determining hla status using immune repertoire sequencing
ES2836802T3 (en) 2015-02-27 2021-06-28 Becton Dickinson Co Spatially addressable molecular barcodes
EP4180535A1 (en) 2015-03-30 2023-05-17 Becton, Dickinson and Company Methods and compositions for combinatorial barcoding
AU2016242967B2 (en) 2015-04-01 2021-07-01 Adaptive Biotechnologies Corp. Method of identifying human compatible T cell receptors specific for an antigenic target
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
ES2745694T3 (en) 2015-09-11 2020-03-03 Cellular Res Inc Methods and compositions for nucleic acid library normalization
WO2017058936A1 (en) * 2015-09-29 2017-04-06 Kapa Biosystems, Inc. High molecular weight dna sample tracking tags for next generation sequencing
KR20180097536A (en) * 2015-11-04 2018-08-31 아트레카, 인크. A combination set of nucleic acid barcodes for the analysis of nucleic acids associated with single cells
EP4269616A3 (en) 2016-05-02 2024-02-14 Becton, Dickinson and Company Accurate molecular barcoding
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
JP7046007B2 (en) 2016-05-26 2022-04-01 ベクトン・ディキンソン・アンド・カンパニー How to adjust the molecular label count
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
SG11201811799VA (en) * 2016-07-01 2019-02-27 Kaneka Corp Primer set, kit and method for detecting two or more target nucleic acids
WO2018018008A1 (en) * 2016-07-22 2018-01-25 Oregon Health & Science University Single cell whole genome libraries and combinatorial indexing methods of making thereof
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
JP7091348B2 (en) 2016-09-26 2022-06-27 ベクトン・ディキンソン・アンド・カンパニー Measurement of protein expression using reagents with barcoded oligonucleotide sequences
AU2017359047A1 (en) 2016-11-08 2019-05-02 Becton, Dickinson And Company Methods for cell label classification
CN109952612B (en) 2016-11-08 2023-12-01 贝克顿迪金森公司 Method for classifying expression profiles
WO2018111835A1 (en) 2016-12-12 2018-06-21 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
EP3568234B1 (en) 2017-01-13 2023-09-06 Cellular Research, Inc. Hydrophilic coating of fluidic channels
EP3545089B1 (en) 2017-01-30 2022-03-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
WO2018144240A1 (en) 2017-02-01 2018-08-09 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
RU2656216C1 (en) * 2017-03-24 2018-06-01 Федеральное государственное бюджетное учреждение науки Институт биоорганической химии им. М.М. Шемякина и Ю.А. Овчинникова Российской академии наук Method for ultra-high-throughput screening of cells or microorganisms and means for ultra-high-throughput screening of cells or microorganisms
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN116064732A (en) 2017-05-26 2023-05-05 10X基因组学有限公司 Single cell analysis of transposase accessibility chromatin
WO2018226293A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
WO2019023243A1 (en) * 2017-07-24 2019-01-31 Dana-Farber Cancer Institute, Inc. Methods and compositions for selecting and amplifying dna targets in a single reaction mixture
CN111051523B (en) 2017-11-15 2024-03-19 10X基因组学有限公司 Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
EP3728636A1 (en) 2017-12-19 2020-10-28 Becton, Dickinson and Company Particles associated with oligonucleotides
CN112262218A (en) 2018-04-06 2021-01-22 10X基因组学有限公司 System and method for quality control in single cell processing
WO2019213294A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
US11365409B2 (en) 2018-05-03 2022-06-21 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
WO2020072380A1 (en) 2018-10-01 2020-04-09 Cellular Research, Inc. Determining 5' transcript sequences
WO2020097315A1 (en) 2018-11-08 2020-05-14 Cellular Research, Inc. Whole transcriptome analysis of single cells using random priming
JP2022511931A (en) 2018-12-13 2022-02-01 ディーエヌエー スクリプト Direct synthesis of oligonucleotides on cells and biomolecules
CN113195717A (en) 2018-12-13 2021-07-30 贝克顿迪金森公司 Selective extension in single cell whole transcriptome analysis
CN111378645B (en) * 2018-12-27 2020-12-01 江苏金斯瑞生物科技有限公司 Gene synthesis method
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
WO2020154247A1 (en) 2019-01-23 2020-07-30 Cellular Research, Inc. Oligonucleotides associated with antibodies
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
EP4004231A1 (en) 2019-07-22 2022-06-01 Becton, Dickinson and Company Single cell chromatin immunoprecipitation sequencing assay
AU2020357191A1 (en) * 2019-10-05 2022-06-02 Mission Bio, Inc. Methods, systems and apparatus for copy number variations and single nucleotide variations simultaneously detected in single-cells
WO2021092386A1 (en) 2019-11-08 2021-05-14 Becton Dickinson And Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
EP4090763A1 (en) 2020-01-13 2022-11-23 Becton Dickinson and Company Methods and compositions for quantitation of proteins and rna
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
CN114807305A (en) * 2022-04-13 2022-07-29 首都医科大学附属北京口腔医院 Method for constructing prokaryotic organism single cell RNA sequencing library

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2492220C (en) * 2001-07-15 2014-03-18 Keck Graduate Institute Nucleic acid amplification using nicking agents
US7862999B2 (en) * 2007-01-17 2011-01-04 Affymetrix, Inc. Multiplex targeted amplification using flap nuclease
US8454906B2 (en) * 2007-07-24 2013-06-04 The Regents Of The University Of California Microfabricated droplet generator for single molecule/cell genetic analysis in engineered monodispersed emulsions
US9068181B2 (en) * 2008-05-23 2015-06-30 The General Hospital Corporation Microfluidic droplet encapsulation
EP2719774B8 (en) * 2008-11-07 2020-04-22 Adaptive Biotechnologies Corporation Methods of monitoring conditions by sequence analysis
US9902950B2 (en) * 2010-10-08 2018-02-27 President And Fellows Of Harvard College High-throughput single cell barcoding

Also Published As

Publication number Publication date
US20150247182A1 (en) 2015-09-03
WO2014018460A1 (en) 2014-01-30
AU2013293240A1 (en) 2015-03-05
JP2015523087A (en) 2015-08-13
CN104540964A (en) 2015-04-22
CA2878694A1 (en) 2014-01-30
EP2877604A1 (en) 2015-06-03

Similar Documents

Publication Publication Date Title
SG11201500313YA (en) Single cell analysis using sequence tags
HK1251033A1 (en) Whole cell assays and methods
HK1211623A1 (en) Cell lines
EP3480321B8 (en) Massively parallel single cell analysis
EP2814984A4 (en) Mirna analysis methods
GB201218555D0 (en) Immunoassay using electrochemical detection
EP2764112A4 (en) Direct nucleic acid analysis
HK1207121A1 (en) Cell culturing device
EP2712451A4 (en) Data analysis system
EP2911230A4 (en) Cell
EP2911216A4 (en) Cell module
EP2878953A4 (en) Analysis device
SG10201700670WA (en) Cell culture
EP2831949A4 (en) Sodium-oxygen cells
EP2891709A4 (en) Nucleic acid analysis device
EP2845006A4 (en) Sample introduction system
EP2812187A4 (en) Color analysis
EP2725341A4 (en) Cell analyzer
HK1199148A1 (en) Electrochemical cell
EP2844773A4 (en) Biomarker analysis using scodaphoresis
EP2821795A4 (en) Sample preprocessing system
EP2884576A4 (en) Cell module
EP2900803A4 (en) Devices and methods for single cell analysis
EP2899655A4 (en) Analysis device
EP2928294A4 (en) Cell preparation method