SG11201407809QA - Chromatographic purification of immunoglobulin g preparations with particles having multimodal functionalities - Google Patents

Chromatographic purification of immunoglobulin g preparations with particles having multimodal functionalities

Info

Publication number
SG11201407809QA
SG11201407809QA SG11201407809QA SG11201407809QA SG11201407809QA SG 11201407809Q A SG11201407809Q A SG 11201407809QA SG 11201407809Q A SG11201407809Q A SG 11201407809QA SG 11201407809Q A SG11201407809Q A SG 11201407809QA SG 11201407809Q A SG11201407809Q A SG 11201407809QA
Authority
SG
Singapore
Prior art keywords
international
chromatographic column
packed
sample
protein
Prior art date
Application number
SG11201407809QA
Inventor
Peter Stanley Gagnon
Original Assignee
Agency Science Tech & Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency Science Tech & Res filed Critical Agency Science Tech & Res
Publication of SG11201407809QA publication Critical patent/SG11201407809QA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/54—F(ab')2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 5 December 2013 (05.12.2013) WIPOIPCT (10) International Publication Number WO 2013/180647 A1 (51) International Patent Classification: C07K1/16 (2006.01) C07K16/00 (2006.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: PCT/SG2013/000031 21 January 2013 (21.01.2013) English English (30) Priority Data: 61/653,913 31 May 2012 (31.05.2012) US (71) Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH [SG/SG]; 1 Fusionpolis Way, #20-10 Connexis, Singapore 138632 (SG). (72) Inventor: GAGNON, Peter, Stanley; 20 Biopolis Way, #06-01 Centres, Singapore 138668 (SG). (74) Agent: SPRUSON & FERGUSON (ASIA) PTE LTD; 1531 Robinson Road Post Office, Singapore 903031 (SG). BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) (81) Designated States (unless otherwise indicated, for every Published: kind of national protection available)-. AE, AG, AL, AM, _ . ^ international search t (Art 21(3) ) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, F 1 1 r- •t o 00 i-H en i-H o CJ o & (54) Title: CHROMATOGRAPHIC PURIFICATION OF IMMUNOGLOBULIN G PREPARATIONS WITH PARTICLES HAV­ ING MULTIMODAL FUNCTIONALITIES (57) Abstract: A method of purifying sample a containing a desired protein includes the steps of (i) providing a packed chromato­ graphic column having positively charged porous particles, (ii) equilibrating the column to the conditions to which the desired pro­ tein in the sample is to elute, (iii) contacting the sample with the packed chromatographic column such that the sample volume ap - plied to the packed chromatographic column is less than or equal to the interparticle space of the positively charged porous particles within the packed chromatographic column, (iv) eluting the desired protein from the packed chromatographic column, where the de­ sired protein is in a purer state and in the conditions to which the packed chromatographic column was equilibrated; where the de - sired protein is an antibody, an antibody fragment, an antibody derivative, or an antibody fusion protein. (No suitable Figure)
SG11201407809QA 2012-05-31 2013-01-21 Chromatographic purification of immunoglobulin g preparations with particles having multimodal functionalities SG11201407809QA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261653913P 2012-05-31 2012-05-31
PCT/SG2013/000031 WO2013180647A1 (en) 2012-05-31 2013-01-21 Chromatographic purification of immunoglobulin g preparations with particles having multimodal functionalities

Publications (1)

Publication Number Publication Date
SG11201407809QA true SG11201407809QA (en) 2014-12-30

Family

ID=49673718

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201407809QA SG11201407809QA (en) 2012-05-31 2013-01-21 Chromatographic purification of immunoglobulin g preparations with particles having multimodal functionalities

Country Status (8)

Country Link
US (1) US9890205B2 (en)
EP (1) EP2855504B1 (en)
JP (1) JP6297029B2 (en)
KR (1) KR20150027060A (en)
CN (1) CN104507955A (en)
IN (1) IN2014DN09962A (en)
SG (1) SG11201407809QA (en)
WO (1) WO2013180647A1 (en)

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KR20150118120A (en) 2013-02-22 2015-10-21 에이전시 포 사이언스, 테크놀로지 앤드 리서치 Materials and methods for removing endotoxins from protein preparations
JP2016509069A (en) * 2013-02-28 2016-03-24 エイジェンシー・フォー・サイエンス，テクノロジー・アンド・リサーチ Protein purification with high conductivity in the presence of nonionic organic polymers
CN105980397A (en) * 2014-02-19 2016-09-28 新加坡科技研究局 Fractionation method
CN106061992A (en) * 2014-02-27 2016-10-26 新加坡科技研究局 Methods for reducing chromatin content in protein preparations by treatment with alkyl cations
WO2015130222A1 (en) 2014-02-27 2015-09-03 Agency For Science, Technology And Research Antibody purification process
EP3674310A1 (en) * 2014-03-10 2020-07-01 Richter Gedeon Nyrt. Immunoglobulin purification using pre-cleaning steps
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Also Published As

Publication number Publication date
EP2855504A1 (en) 2015-04-08
EP2855504B1 (en) 2018-10-24
US9890205B2 (en) 2018-02-13
WO2013180647A1 (en) 2013-12-05
JP2015519360A (en) 2015-07-09
KR20150027060A (en) 2015-03-11
US20150183852A1 (en) 2015-07-02
EP2855504A4 (en) 2015-07-29
CN104507955A (en) 2015-04-08
IN2014DN09962A (en) 2015-08-14
JP6297029B2 (en) 2018-03-20

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