SG10201807112XA - Analysis of nucleic acids associated with single cells using nucleic acid barcodes - Google Patents

Analysis of nucleic acids associated with single cells using nucleic acid barcodes

Info

Publication number
SG10201807112XA
SG10201807112XA SG10201807112XA SG10201807112XA SG10201807112XA SG 10201807112X A SG10201807112X A SG 10201807112XA SG 10201807112X A SG10201807112X A SG 10201807112XA SG 10201807112X A SG10201807112X A SG 10201807112XA SG 10201807112X A SG10201807112X A SG 10201807112XA
Authority
SG
Singapore
Prior art keywords
single cells
nucleic acid
sample
nucleic acids
analysis
Prior art date
Application number
SG10201807112XA
Inventor
Yann Chong Tan
Gary Withey
Original Assignee
Atreca Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Atreca Inc filed Critical Atreca Inc
Publication of SG10201807112XA publication Critical patent/SG10201807112XA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/131—Modifications characterised by incorporating a restriction site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

ANALYSIS OF NUCLEIC ACIDS ASSOCIATED WITH SINGLE CELLS USING NUCLEIC ACID BARCODES Provided herein are methods and compositions for analyzing nucleic acids associated with single cells using nucleic acid barcodes. According to some embodiments, a method for producing one or more polynucleotides of interest comprises: obtaining a plurality of RNAs associated with one or more samples, wherein the samples are obtained from one or more subjects, each RNA is associated with a single sample, and the RNAs associated with each sample are present in a separate reaction volume; adding an adapter molecule to the RNAs associated with each sample, wherein the adapter molecule is generated using an enzymatic reaction and comprises a universal priming sequence, a barcode sequence, and a binding site; and incorporating the barcode sequence into one or more polynucleotides associated with each sample, thereby producing the one or more polynucleotides of interest. No suitable figure
SG10201807112XA 2013-12-30 2014-12-30 Analysis of nucleic acids associated with single cells using nucleic acid barcodes SG10201807112XA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201361922012P 2013-12-30 2013-12-30

Publications (1)

Publication Number Publication Date
SG10201807112XA true SG10201807112XA (en) 2018-09-27

Family

ID=52424112

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201605344YA SG11201605344YA (en) 2013-12-30 2014-12-30 Analysis of nucleic acids associated with single cells using nucleic acid barcodes
SG10201807112XA SG10201807112XA (en) 2013-12-30 2014-12-30 Analysis of nucleic acids associated with single cells using nucleic acid barcodes

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG11201605344YA SG11201605344YA (en) 2013-12-30 2014-12-30 Analysis of nucleic acids associated with single cells using nucleic acid barcodes

Country Status (13)

Country Link
US (6) US9580736B2 (en)
EP (2) EP3089822B1 (en)
JP (1) JP6608368B2 (en)
KR (2) KR20220119751A (en)
CN (2) CN114717291A (en)
AU (1) AU2014373757B2 (en)
CA (1) CA2935122C (en)
DK (1) DK3089822T3 (en)
ES (1) ES2912183T3 (en)
PL (1) PL3089822T3 (en)
PT (1) PT3089822T (en)
SG (2) SG11201605344YA (en)
WO (1) WO2015103339A1 (en)

Families Citing this family (150)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
WO2011146632A1 (en) 2010-05-18 2011-11-24 Gene Security Network Inc. Methods for non-invasive prenatal ploidy calling
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
AU2012212148B8 (en) 2011-02-02 2017-07-06 University Of Washington Through Its Center For Commercialization Massively parallel contiguity mapping
CN104364392B (en) 2012-02-27 2018-05-25 赛卢拉研究公司 For the composition and kit of numerator counts
EP2823064B1 (en) 2012-03-05 2019-02-06 President and Fellows of Harvard College Methods for epigenetic sequencing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
IN2015DN01126A (en) 2012-08-14 2015-06-26 10X Genomics Inc
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
DK2954065T3 (en) 2013-02-08 2021-09-06 10X Genomics Inc Division and processing of analytes and other species
AU2013382098B2 (en) 2013-03-13 2019-02-07 Illumina, Inc. Methods and compositions for nucleic acid sequencing
GB2584364A (en) * 2013-03-15 2020-12-02 Abvitro Llc Single cell bar-coding for antibody discovery
KR20230074639A (en) 2013-08-28 2023-05-30 벡톤 디킨슨 앤드 컴퍼니 Massively parallel single cell analysis
CN105745528A (en) 2013-10-07 2016-07-06 赛卢拉研究公司 Methods and systems for digitally counting features on arrays
US10246746B2 (en) 2013-12-20 2019-04-02 Illumina, Inc. Preserving genomic connectivity information in fragmented genomic DNA samples
WO2015103339A1 (en) 2013-12-30 2015-07-09 Atreca, Inc. Analysis of nucleic acids associated with single cells using nucleic acid barcodes
CN114534806B (en) 2014-04-10 2024-03-29 10X基因组学有限公司 Fluidic devices, systems and methods for packaging and partitioning reagents and uses thereof
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
DK3456846T3 (en) * 2014-04-21 2022-07-11 Harvard College SYSTEMS AND METHODS FOR BARCODE MARKING NUCLEIC ACID
EP3134541B1 (en) 2014-04-21 2020-08-19 Natera, Inc. Detecting copy number variations (cnv) of chromosomal segments in cancer
CA3060708A1 (en) * 2014-04-29 2015-11-05 Illumina, Inc Multiplexed single cell gene expression analysis using template switch and tagmentation
US10975371B2 (en) * 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
KR20230070325A (en) 2014-06-26 2023-05-22 10엑스 제노믹스, 인크. Methods of analyzing nucleic acids from individual cells or cell populations
MX2017003382A (en) * 2014-09-15 2017-11-20 Abvitro Llc High-throughput nucleotide library sequencing.
JP6808617B2 (en) 2014-10-17 2021-01-06 イルミナ ケンブリッジ リミテッド Dislocations that maintain continuity
CN107969137B (en) * 2014-10-17 2021-10-26 伊卢米纳剑桥有限公司 Proximity-preserving transposons
CN107002128A (en) 2014-10-29 2017-08-01 10X 基因组学有限公司 The method and composition being sequenced for target nucleic acid
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
BR112017014902A2 (en) 2015-01-12 2018-03-13 10X Genomics Inc processes and systems for the preparation of nucleic acid sequencing libraries and libraries prepared using them
EP3259371B1 (en) 2015-02-19 2020-09-02 Becton, Dickinson and Company High-throughput single-cell analysis combining proteomic and genomic information
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
WO2016138148A1 (en) 2015-02-24 2016-09-01 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
CN107208157B (en) * 2015-02-27 2022-04-05 贝克顿迪金森公司 Methods and compositions for barcoding nucleic acids for sequencing
ES2836802T3 (en) 2015-02-27 2021-06-28 Becton Dickinson Co Spatially addressable molecular barcodes
EP4180535A1 (en) 2015-03-30 2023-05-17 Becton, Dickinson and Company Methods and compositions for combinatorial barcoding
CA2983122A1 (en) 2015-04-17 2016-10-20 President And Fellows Of Harvard College Barcoding systems and methods for gene sequencing and other applications
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
EP3294906A1 (en) 2015-05-11 2018-03-21 Natera, Inc. Methods and compositions for determining ploidy
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
JP6743150B2 (en) * 2015-08-28 2020-08-19 イルミナ インコーポレイテッド Single cell nucleic acid sequence analysis
ES2745694T3 (en) 2015-09-11 2020-03-03 Cellular Res Inc Methods and compositions for nucleic acid library normalization
BR112018005937A2 (en) * 2015-09-24 2019-05-21 Abvitro Llc affinity oligonucleotide conjugates and uses of these
KR20180097536A (en) * 2015-11-04 2018-08-31 아트레카, 인크. A combination set of nucleic acid barcodes for the analysis of nucleic acids associated with single cells
US20170141793A1 (en) * 2015-11-13 2017-05-18 Microsoft Technology Licensing, Llc Error correction for nucleotide data stores
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
WO2017117358A1 (en) * 2015-12-30 2017-07-06 Bio-Rad Laboratories, Inc. Digital protein quantification
EP3402596A1 (en) * 2016-01-14 2018-11-21 European Molecular Biology Laboratory Microfluidic analysis of ligand induced cell expression
WO2017181161A1 (en) * 2016-04-15 2017-10-19 Predicine, Inc. Systems and methods for detecting genetic alterations
EP4269616A3 (en) 2016-05-02 2024-02-14 Becton, Dickinson and Company Accurate molecular barcoding
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
WO2017205344A1 (en) * 2016-05-25 2017-11-30 Bio-Rad Laboratories, Inc. Digital proximity assay
JP7046007B2 (en) 2016-05-26 2022-04-01 ベクトン・ディキンソン・アンド・カンパニー How to adjust the molecular label count
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
CN105950612B (en) * 2016-07-08 2019-06-21 北京全式金生物技术有限公司 A kind of efficient DNA connector connecting method
CN109642219B (en) 2016-08-05 2021-02-26 生物辐射实验室股份有限公司 Second chain guide
CN116445593A (en) * 2016-08-10 2023-07-18 格里尔公司 Method for determining a methylation profile of a biological sample
JP7091348B2 (en) 2016-09-26 2022-06-27 ベクトン・ディキンソン・アンド・カンパニー Measurement of protein expression using reagents with barcoded oligonucleotide sequences
US11485996B2 (en) 2016-10-04 2022-11-01 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
WO2018075693A1 (en) * 2016-10-19 2018-04-26 10X Genomics, Inc. Methods and systems for barcoding nucleic acid molecules from individual cells or cell populations
WO2018081113A1 (en) 2016-10-24 2018-05-03 Sawaya Sterling Concealing information present within nucleic acids
AU2017359047A1 (en) 2016-11-08 2019-05-02 Becton, Dickinson And Company Methods for cell label classification
CN109952612B (en) 2016-11-08 2023-12-01 贝克顿迪金森公司 Method for classifying expression profiles
CN110050067B (en) * 2016-11-10 2023-05-23 宝生物工程（美国)有限公司 Methods of producing amplified double-stranded deoxyribonucleic acid, and compositions and kits for use in the methods
US10011870B2 (en) * 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) * 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA3046379A1 (en) * 2016-12-22 2018-06-28 Illumina, Inc Arrays with quality control tracers
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3568234B1 (en) 2017-01-13 2023-09-06 Cellular Research, Inc. Hydrophilic coating of fluidic channels
EP3545089B1 (en) 2017-01-30 2022-03-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
WO2018144240A1 (en) 2017-02-01 2018-08-09 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
EP3375889B1 (en) * 2017-03-17 2019-12-11 HiFiBiO SAS Single cell analysis
WO2018195493A1 (en) * 2017-04-21 2018-10-25 Mesa Biotech, Inc. Fluidic test cassette
CN110168087B (en) * 2017-04-27 2023-11-14 深圳华大基因股份有限公司 DNA tag and application thereof
US11584951B2 (en) 2017-05-02 2023-02-21 The University Of Tokyo Method for integrally detecting nondestructive measurement information and genome-related information of one cell
CN111148846A (en) * 2017-05-05 2020-05-12 西比欧生物科学公司 Method for capturing and barcoding discrete biological units in hydrogels
CN116064732A (en) 2017-05-26 2023-05-05 10X基因组学有限公司 Single cell analysis of transposase accessibility chromatin
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
WO2018226293A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
CN107502607A (en) * 2017-06-20 2017-12-22 浙江大学 A kind of a large amount of tissues, cell sample mRNA molecular barcode mark, library construction, the method for sequencing
WO2019060716A1 (en) 2017-09-25 2019-03-28 Freenome Holdings, Inc. Methods and systems for sample extraction
US20190153438A1 (en) * 2017-11-15 2019-05-23 Viome, Inc. Methods and compositions for preparing polynucleotide libraries
CN111051523B (en) 2017-11-15 2024-03-19 10X基因组学有限公司 Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
EP3717661A1 (en) 2017-11-27 2020-10-07 The Trustees of Columbia University in the City of New York Rna printing and sequencing devices, methods, and systems
WO2019113563A1 (en) * 2017-12-09 2019-06-13 Viome, Inc. Methods for nucleic acid library creation
EP3728636A1 (en) * 2017-12-19 2020-10-28 Becton, Dickinson and Company Particles associated with oligonucleotides
KR102014470B1 (en) 2017-12-29 2019-08-26 한국과학기술원 Method for detecting target RNA utilizing Nicking/Extension chain reaction System-Based isothermal nucleic acid Amplification (NESBA)
WO2019133874A1 (en) * 2017-12-31 2019-07-04 Berkeley Lights, Inc. General functional assay
WO2019147663A1 (en) * 2018-01-24 2019-08-01 Freenome Holdings, Inc. Methods and systems for abnormality detection in the patterns of nucleic acids
CN112005115A (en) * 2018-02-12 2020-11-27 10X基因组学有限公司 Methods of characterizing multiple analytes from a single cell or cell population
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
CN112074610A (en) * 2018-02-22 2020-12-11 10X基因组学有限公司 Conjugation-mediated nucleic acid analysis
CN112236518A (en) * 2018-02-23 2021-01-15 耶鲁大学 Single cell freeze-thaw lysis
EP3762494B1 (en) * 2018-03-07 2022-11-23 Wisconsin Alumni Research Foundation High throughput nucleic acid profiling of single cells
CN112262218A (en) 2018-04-06 2021-01-22 10X基因组学有限公司 System and method for quality control in single cell processing
US11365409B2 (en) 2018-05-03 2022-06-21 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
WO2019213294A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
EP3794141A4 (en) * 2018-05-17 2022-02-23 Illumina, Inc. High-throughput single-cell sequencing with reduced amplification bias
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
WO2020046833A1 (en) * 2018-08-28 2020-03-05 Cellular Research, Inc. Sample multiplexing using carbohydrate-binding and membrane-permeable reagents
WO2020072380A1 (en) * 2018-10-01 2020-04-09 Cellular Research, Inc. Determining 5' transcript sequences
GB2614128B (en) * 2018-10-05 2024-02-28 Univ Texas Solid-phase N-terminal peptide capture and release
EP3878936A4 (en) 2018-11-07 2022-08-10 The University of Tokyo Method for detecting genome-related information of cell coexisting with one or more substances to be detected
WO2020097315A1 (en) 2018-11-08 2020-05-14 Cellular Research, Inc. Whole transcriptome analysis of single cells using random priming
WO2020118200A1 (en) * 2018-12-07 2020-06-11 Qiagen Sciences, Llc Methods for preparing cdna samples for rna sequencing, and cdna samples and uses thereof
US11459607B1 (en) * 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
CN113195717A (en) 2018-12-13 2021-07-30 贝克顿迪金森公司 Selective extension in single cell whole transcriptome analysis
US11358137B2 (en) 2018-12-26 2022-06-14 Industrial Technology Research Institute Tubular structure for producing droplets and method for producing droplets
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
WO2020154247A1 (en) 2019-01-23 2020-07-30 Cellular Research, Inc. Oligonucleotides associated with antibodies
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
CN110241460A (en) * 2019-05-31 2019-09-17 南方医科大学南方医院 A kind of immune group library method for screening the reaction of independent sample crossover
EP4004231A1 (en) 2019-07-22 2022-06-01 Becton, Dickinson and Company Single cell chromatin immunoprecipitation sequencing assay
EP4013863A4 (en) * 2019-08-14 2023-08-30 iRepertoire, Inc. Probe-capture method for tcr alpha and beta chain vdj-recovery from oligo-dt reverse transcribed rna
EP4041310A4 (en) 2019-10-10 2024-05-15 1859 Inc Methods and systems for microfluidic screening
WO2021092386A1 (en) 2019-11-08 2021-05-14 Becton Dickinson And Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
EP4090763A1 (en) 2020-01-13 2022-11-23 Becton Dickinson and Company Methods and compositions for quantitation of proteins and rna
WO2021146184A1 (en) * 2020-01-13 2021-07-22 Fluent Biosciences Inc. Single cell sequencing
US11512337B2 (en) 2020-01-13 2022-11-29 Fluent Biosciences Inc. Emulsion based drug screening
US11827936B2 (en) 2020-01-13 2023-11-28 Fluent Biosciences Inc. Methods and systems for single cell gene profiling
US20230220376A1 (en) 2020-01-22 2023-07-13 Atreca, Inc. High throughput linking of multiple transcripts
US11866782B2 (en) 2020-03-16 2024-01-09 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
TW202242111A (en) * 2020-12-18 2022-11-01 美商Ionis製藥公司 Compounds and methods for modulating factor xii
IL303828A (en) * 2020-12-22 2023-08-01 Hifibio Sas Microfluidic methods and systems
AU2022227563A1 (en) 2021-02-23 2023-08-24 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
WO2023091884A1 (en) * 2021-11-16 2023-05-25 Pioneer Hi-Bred International, Inc. Maize event das-01131-3 and methods for detection thereof
EP4353825A1 (en) * 2022-10-10 2024-04-17 bisy GmbH Modified promoter sequences

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5624711A (en) * 1995-04-27 1997-04-29 Affymax Technologies, N.V. Derivatization of solid supports and methods for oligomer synthesis
US5928906A (en) 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
CA2438856C (en) 2001-02-23 2007-08-07 Japan Science And Technology Corporation Process and apparatus for producing emulsion and microcapsules
US7198897B2 (en) * 2001-12-19 2007-04-03 Brandeis University Late-PCR
US7976779B2 (en) * 2002-06-26 2011-07-12 California Institute Of Technology Integrated LC-ESI on a chip
US9045796B2 (en) * 2003-06-20 2015-06-02 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
TWI333977B (en) 2003-09-18 2010-12-01 Symphogen As Method for linking sequences of interest
CN100431679C (en) 2004-03-23 2008-11-12 独立行政法人科学技术振兴机构 Method and device for producing micro-droplets
JOP20080381B1 (en) 2007-08-23 2023-03-28 Amgen Inc Antigen Binding Proteins to Proprotein Convertase subtillisin Kexin type 9 (pcsk9)
EP2036989B1 (en) * 2007-09-12 2012-07-25 Institut Pasteur Polynucleotide suitable for single cell based reporter assay to monitor gene expression patterns with high spatio-temporal resolution
WO2010014820A2 (en) * 2008-07-30 2010-02-04 Life Technologies Corporation Particles for use in supported nucleic acid ligation and detection sequencing
US9625454B2 (en) 2009-09-04 2017-04-18 The Research Foundation For The State University Of New York Rapid and continuous analyte processing in droplet microfluidic devices
EP2420579A1 (en) * 2010-08-17 2012-02-22 QIAGEN GmbH Helicase dependent isothermal amplification using nicking enzymes
EP2614154B1 (en) * 2010-09-10 2014-12-17 New England Biolabs, Inc. Method for reducing adapter-dimer formation
US9902950B2 (en) * 2010-10-08 2018-02-27 President And Fellows Of Harvard College High-throughput single cell barcoding
DK2625295T3 (en) 2010-10-08 2019-06-11 Harvard College HIGH-THROUGHPUT-IMMUNE SEQUENCING
EP2652155B1 (en) 2010-12-16 2016-11-16 Gigagen, Inc. Methods for massively parallel analysis of nucleic acids in single cells
US9150852B2 (en) * 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
US9260753B2 (en) 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
US20140141442A1 (en) * 2011-04-05 2014-05-22 Institut National De La Sante Et De La Recherche Medicale (Inserm) Linear dna amplification
EP3415619B1 (en) 2011-04-28 2020-12-16 The Board of Trustees of the Leland Stanford Junior University Identification of polynucleotides associated with a sample
US20150125865A1 (en) 2011-12-23 2015-05-07 Gigagen, Inc. Methods And Apparatuses For Droplet Mixing
US9176031B2 (en) * 2012-02-24 2015-11-03 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
EP2823064B1 (en) 2012-03-05 2019-02-06 President and Fellows of Harvard College Methods for epigenetic sequencing
NO2694769T3 (en) * 2012-03-06 2018-03-03
JP2015519900A (en) * 2012-05-21 2015-07-16 フリューダイム・コーポレイション Single particle analysis method and single particle isolation method for particle population
WO2013188872A1 (en) 2012-06-15 2013-12-19 The Board Of Regents Of The University Of Texas System High throughput sequencing of multiple transcripts of a single cell
WO2014108850A2 (en) * 2013-01-09 2014-07-17 Yeda Research And Development Co. Ltd. High throughput transcriptome analysis
CN105579587A (en) * 2013-08-23 2016-05-11 惠氏公司 Methods and compositions for cDNA synthesis and single-cell transcriptome profiling using template switching reaction
GB201317301D0 (en) * 2013-09-30 2013-11-13 Linnarsson Sten Method for capturing and encoding nucleic acid from a plurality of single cells
WO2015103339A1 (en) 2013-12-30 2015-07-09 Atreca, Inc. Analysis of nucleic acids associated with single cells using nucleic acid barcodes
US20150298091A1 (en) * 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids

Also Published As

Publication number Publication date
US20150329891A1 (en) 2015-11-19
AU2014373757A1 (en) 2016-07-07
SG11201605344YA (en) 2016-07-28
EP4094834A1 (en) 2022-11-30
CN106460033A (en) 2017-02-22
PT3089822T (en) 2022-05-06
PL3089822T3 (en) 2022-09-19
KR20220119751A (en) 2022-08-30
US20220243240A1 (en) 2022-08-04
US20170369921A1 (en) 2017-12-28
WO2015103339A1 (en) 2015-07-09
CA2935122C (en) 2023-09-19
US9580736B2 (en) 2017-02-28
AU2014373757B2 (en) 2019-12-12
CN114717291A (en) 2022-07-08
KR102433825B1 (en) 2022-08-31
DK3089822T3 (en) 2022-05-02
JP2017506877A (en) 2017-03-16
US20220389472A1 (en) 2022-12-08
US20200123582A1 (en) 2020-04-23
US10316345B2 (en) 2019-06-11
EP3089822B1 (en) 2022-04-06
EP3089822A1 (en) 2016-11-09
KR20160108377A (en) 2016-09-19
JP6608368B2 (en) 2019-11-20
ES2912183T3 (en) 2022-05-24
CN106460033B (en) 2021-12-24
US20220389471A1 (en) 2022-12-08
CA2935122A1 (en) 2015-07-09

Similar Documents

Publication Publication Date Title
SG10201807112XA (en) Analysis of nucleic acids associated with single cells using nucleic acid barcodes
GB2513793A (en) Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
EP4265723A3 (en) Reagents and methods for molecular barcoding of nucleic acids of single cells
IN2015DN01126A (en)
WO2016033251A3 (en) Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
EP4321628A3 (en) Transposition into native chromatin for personal epigenomics
WO2012011660A3 (en) Method of manufacturing micro chamber plate with built-in sample and analytic micro chamber plate, analytic micro chamber plate and apparatus set for manufacturing analytic micro chamber plate with built-in sample
WO2017143155A3 (en) Multiplex alteration of cells using a pooled nucleic acid library and analysis thereof
WO2011071923A3 (en) Multi-sample indexing for multiplex genotyping
GB2487341A (en) Compositions and methods for targeted nucleic acid sequence selection and amplification
EP2759344A3 (en) Microfluidic System for Nucleic Acid Analysis
WO2012135815A3 (en) Methods and kits for detecting cell-free pathogen-specific nucleic acids
EA201101141A1 (en) OBTAINING CLOSED LINEAR DNA
CA2884084C (en) Fad2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks
CN102719526A (en) MicroRNA quantitative detection analytic method by utilizing isothermal amplification to synthesize fluorescent nano silver cluster probe
WO2015095226A3 (en) Preserving genomic connectivity information in fragmented genomic dna samples
WO2012042374A3 (en) Method of determining number or concentration of molecules
WO2010107816A3 (en) Nucleic acid binding dyes and uses therefor
WO2011101728A3 (en) Identification of source of dna samples
WO2018053365A8 (en) Methods of nucleic acid sample preparation for analysis of cell-free dna
WO2014153193A9 (en) Diagnostic systems and methods
WO2007140015A3 (en) Biochemical analysis of partitioned cells
NZ588036A (en) Analysis of dna by means of capillary gel electrophoresis
WO2011132061A3 (en) Methylation profiling of dna samples
McPherson A defining decade in DNA sequencing