SG10201710439UA - Methods of purifying polypeptides - Google Patents

Methods of purifying polypeptides

Info

Publication number
SG10201710439UA
SG10201710439UA SG10201710439UA SG10201710439UA SG10201710439UA SG 10201710439U A SG10201710439U A SG 10201710439UA SG 10201710439U A SG10201710439U A SG 10201710439UA SG 10201710439U A SG10201710439U A SG 10201710439UA SG 10201710439U A SG10201710439U A SG 10201710439UA
Authority
SG
Singapore
Prior art keywords
methods
purifying polypeptides
polypeptides
purifying
Prior art date
Application number
SG10201710439UA
Inventor
Hui F Liu
Brian David Kelley
Deanna E Myers
Beth Mccooey
Krista Marie Petty
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=45004368&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=SG10201710439U(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of SG10201710439UA publication Critical patent/SG10201710439UA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/18Ion-exchange chromatography
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361Ion-exchange
    • B01D15/362Cation-exchange
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3847Multimodal interactions
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/165Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/22Affinity chromatography or related techniques based upon selective absorption processes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02Column chromatography
    • G01N30/88Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
SG10201710439UA 2010-05-25 2011-05-25 Methods of purifying polypeptides SG10201710439UA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US34814310P 2010-05-25 2010-05-25

Publications (1)

Publication Number Publication Date
SG10201710439UA true SG10201710439UA (en) 2018-01-30

Family

ID=45004368

Family Applications (2)

Application Number Title Priority Date Filing Date
SG2012086419A SG185737A1 (en) 2010-05-25 2011-05-25 Methods of purifying polypeptides
SG10201710439UA SG10201710439UA (en) 2010-05-25 2011-05-25 Methods of purifying polypeptides

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG2012086419A SG185737A1 (en) 2010-05-25 2011-05-25 Methods of purifying polypeptides

Country Status (21)

Country Link
US (4) US20130079272A1 (en)
EP (2) EP3299380A1 (en)
JP (3) JP5934190B2 (en)
KR (1) KR101976853B1 (en)
CN (2) CN107365346A (en)
AU (1) AU2011258254B2 (en)
BR (1) BR112012029904A2 (en)
CA (2) CA3209878A1 (en)
ES (1) ES2637613T5 (en)
HK (1) HK1246328A1 (en)
HR (1) HRP20171095T4 (en)
IL (2) IL223029A0 (en)
MX (2) MX344248B (en)
MY (1) MY161534A (en)
NZ (1) NZ603648A (en)
PL (1) PL2575847T5 (en)
RU (1) RU2594163C2 (en)
SG (2) SG185737A1 (en)
SI (1) SI2575847T2 (en)
WO (1) WO2011150110A1 (en)
ZA (1) ZA201208673B (en)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2767135T3 (en) * 2011-10-19 2020-06-16 Novimmune Sa Methods for purifying antibodies
MY172426A (en) 2011-12-22 2019-11-25 Genentech Inc Ion exchange membrane chromatography
CN102911250B (en) * 2012-09-29 2014-04-16 浙江海正药业股份有限公司 Method for purifying acidic recombinant protein medicament
CN103880947B (en) * 2012-12-21 2016-01-13 武汉禾元生物科技股份有限公司 A kind of chromatography method of separating and purifying high-purity recombination human serum albumin
WO2014118685A2 (en) * 2013-01-29 2014-08-07 Dr. Reddy's Laboratories Limited Method of altering the acidic variant content of antibody
WO2014143616A1 (en) * 2013-03-14 2014-09-18 Qiagen Sciences Llc Assessing dna quality using real-time pcr and ct values
CN104236983A (en) * 2013-06-14 2014-12-24 中国科学院大连化学物理研究所 Method for removing ionic liquid containing long alkyl chain in solution sample
WO2015035180A1 (en) * 2013-09-05 2015-03-12 Genentech, Inc. Method for chromatography reuse
CA3174012A1 (en) 2013-09-13 2015-03-19 Genentech, Inc. Compositions and methods for detecting and quantifying host cell protein in cell lines and recombinant polypeptide products
CA3184564A1 (en) 2013-09-13 2015-03-19 Genentech, Inc. Methods and compositions comprising an anti-il13 antibody and residual hamster phospholipase b-like 2
US10376582B2 (en) 2013-10-16 2019-08-13 Outlook Therapeutics, Inc. Buffer formulations for enhanced antibody stability
US8859252B1 (en) 2014-01-02 2014-10-14 Aerial Biopharma, Llc Prostatic acid phosphatase, compositions comprising the same, and methods for producing and/or purifying the same
US10214747B2 (en) 2014-01-09 2019-02-26 Kentucky Bioprocessing, Inc. Method of purifying monoclonal antibodies
EP3674310A1 (en) 2014-03-10 2020-07-01 Richter Gedeon Nyrt. Immunoglobulin purification using pre-cleaning steps
ES2719748T3 (en) 2014-05-23 2019-07-12 Inst Massone S A Process to obtain HMG-UP (human menopausal gonadotropin with ultrapure grade) and a contaminant-free composition
WO2015198320A1 (en) * 2014-06-24 2015-12-30 Insight Biopharmaceuticals Ltd. Methods of purifying antibodies
GB201411802D0 (en) 2014-07-02 2014-08-13 Univ Bradford Effervescent compositions
US10696735B2 (en) * 2015-01-21 2020-06-30 Outlook Therapeutics, Inc. Modulation of charge variants in a monoclonal antibody composition
EP3411401A1 (en) 2016-02-03 2018-12-12 Oncobiologics, Inc. Buffer formulations for enhanced antibody stability
WO2017218977A2 (en) 2016-06-17 2017-12-21 Genentech, Inc. Purification of multispecific antibodies
MX2019001572A (en) 2016-08-15 2019-08-29 Genentech Inc Chromatography method for quantifying a non-ionic surfactant in a composition comprising the non-ionic surfactant and a polypeptide.
JP6564539B1 (en) 2018-09-14 2019-08-21 長瀬産業株式会社 Peptide purification method using sulfonic acid compound
WO2020132452A1 (en) 2018-12-20 2020-06-25 Shire Human Genetic Therapies, Inc. Purification of iduronate-2-sulfatase immunoglobulin fusion protein
CN109765307B (en) * 2018-12-24 2021-06-29 山东省科学院海洋仪器仪表研究所 Method for measuring hexachlorocyclohexane in water body
CN111366547A (en) * 2018-12-26 2020-07-03 贵州中烟工业有限责任公司 Detection method for determining α -amino nitrogen in tobacco or tobacco products by using continuous flow method
CN113302197A (en) 2019-01-23 2021-08-24 第一三共株式会社 Method for purifying antibody including process using activated carbon material
TW202112799A (en) * 2019-06-05 2021-04-01 美商建南德克公司 A method for regeneration of an overload chromatography column
WO2021076489A1 (en) * 2019-10-14 2021-04-22 Pierce Biotechnology, Inc. Peptide purification formulations and methods
US20230251269A1 (en) * 2020-07-15 2023-08-10 The Johns Hopkins University Comprehensive analysis of anti-allergen antibodies using phage display
CN112852876B (en) * 2021-03-04 2022-11-29 西南大学 Silkworm silk gland recombinant expression vector for expressing human epidermal growth factor and preparation method and application thereof
KR20230165245A (en) 2021-03-31 2023-12-05 에프. 호프만-라 로슈 아게 Purification of antibodies by mixed mode chromatography

Family Cites Families (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4704362A (en) 1977-11-08 1987-11-03 Genentech, Inc. Recombinant cloning vehicle microbial polypeptide expression
FR2413974A1 (en) 1978-01-06 1979-08-03 David Bernard DRYER FOR SCREEN-PRINTED SHEETS
US4399216A (en) 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4419446A (en) 1980-12-31 1983-12-06 The United States Of America As Represented By The Department Of Health And Human Services Recombinant DNA process utilizing a papilloma virus DNA as a vector
NZ201705A (en) 1981-08-31 1986-03-14 Genentech Inc Recombinant dna method for production of hepatitis b surface antigen in yeast
US4601978A (en) 1982-11-24 1986-07-22 The Regents Of The University Of California Mammalian metallothionein promoter system
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US4965199A (en) 1984-04-20 1990-10-23 Genentech, Inc. Preparation of functional human factor VIII in mammalian cells using methotrexate based selection
US4676980A (en) 1985-09-23 1987-06-30 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Target specific cross-linked heteroantibodies
US5567610A (en) 1986-09-04 1996-10-22 Bioinvent International Ab Method of producing human monoclonal antibodies and kit therefor
IL85035A0 (en) 1987-01-08 1988-06-30 Int Genetic Eng Polynucleotide molecule,a chimeric antibody with specificity for human b cell surface antigen,a process for the preparation and methods utilizing the same
DE3889546T2 (en) 1987-12-21 1994-09-08 Univ Toledo TRANSFORMATION OF Germinating PLANT SEEDS WITH THE HELP OF AGROBACTERIUM.
GB8823869D0 (en) 1988-10-12 1988-11-16 Medical Res Council Production of antibodies
US5175384A (en) 1988-12-05 1992-12-29 Genpharm International Transgenic mice depleted in mature t-cells and methods for making transgenic mice
FR2646437B1 (en) 1989-04-28 1991-08-30 Transgene Sa NOVEL DNA SEQUENCES, THEIR APPLICATION AS A SEQUENCE ENCODING A SIGNAL PEPTIDE FOR THE SECRETION OF MATURE PROTEINS BY RECOMBINANT YEASTS, EXPRESSION CASSETTES, PROCESSED YEASTS AND PROCESS FOR PREPARING THE SAME
EP0402226A1 (en) 1989-06-06 1990-12-12 Institut National De La Recherche Agronomique Transformation vectors for yeast yarrowia
DE3920358A1 (en) 1989-06-22 1991-01-17 Behringwerke Ag BISPECIFIC AND OLIGO-SPECIFIC, MONO- AND OLIGOVALENT ANTI-BODY CONSTRUCTS, THEIR PRODUCTION AND USE
ATE144793T1 (en) 1989-06-29 1996-11-15 Medarex Inc BISPECIFIC REAGENTS FOR AIDS THERAPY
US5208020A (en) 1989-10-25 1993-05-04 Immunogen Inc. Cytotoxic agents comprising maytansinoids and their therapeutic use
US5229275A (en) 1990-04-26 1993-07-20 Akzo N.V. In-vitro method for producing antigen-specific human monoclonal antibodies
US5571894A (en) 1991-02-05 1996-11-05 Ciba-Geigy Corporation Recombinant antibodies specific for a growth factor receptor
EP0586505A1 (en) 1991-05-14 1994-03-16 Repligen Corporation Heteroconjugate antibodies for treatment of hiv infection
DE69233254T2 (en) 1991-06-14 2004-09-16 Genentech, Inc., South San Francisco Humanized Heregulin antibody
MX9204374A (en) 1991-07-25 1993-03-01 Idec Pharma Corp RECOMBINANT ANTIBODY AND METHOD FOR ITS PRODUCTION.
ES2136092T3 (en) 1991-09-23 1999-11-16 Medical Res Council PROCEDURES FOR THE PRODUCTION OF HUMANIZED ANTIBODIES.
US5587458A (en) 1991-10-07 1996-12-24 Aronex Pharmaceuticals, Inc. Anti-erbB-2 antibodies, combinations thereof, and therapeutic and diagnostic uses thereof
WO1993008829A1 (en) 1991-11-04 1993-05-13 The Regents Of The University Of California Compositions that mediate killing of hiv-infected cells
CA2122732C (en) 1991-11-25 2008-04-08 Marc D. Whitlow Multivalent antigen-binding proteins
DE69333807T2 (en) 1992-02-06 2006-02-02 Chiron Corp., Emeryville MARKERS FOR CANCER AND BIOSYNTHETIC BINDEPROTEIN THEREFOR
JP3571337B2 (en) 1992-02-11 2004-09-29 セル ジェネシス,インコーポレーテッド Homozygous gene conjugation by gene targeting
US5573905A (en) 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
CA2140280A1 (en) 1992-08-17 1994-03-03 Avi J. Ashkenazi Bispecific immunoadhesins
US5736137A (en) 1992-11-13 1998-04-07 Idec Pharmaceuticals Corporation Therapeutic application of chimeric and radiolabeled antibodies to human B lymphocyte restricted differentiation antigen for treatment of B cell lymphoma
JP3095175B2 (en) 1992-11-13 2000-10-03 アイデック ファーマシューティカルズ コーポレイション Therapeutic use of chimeric and radiolabeled antibodies against human B lymphocyte restricted differentiation antigen for the treatment of B cell lymphoma
US5545328A (en) * 1993-09-21 1996-08-13 Hemosol Inc. Purification of hemoglobin by displacement chromatography
CA2106612C (en) * 1993-09-21 2001-02-06 Diana Pliura Displacement chromatography process
US5534615A (en) 1994-04-25 1996-07-09 Genentech, Inc. Cardiac hypertrophy factor and uses therefor
US5731168A (en) 1995-03-01 1998-03-24 Genentech, Inc. Method for making heteromultimeric polypeptides
US5840523A (en) 1995-03-01 1998-11-24 Genetech, Inc. Methods and compositions for secretion of heterologous polypeptides
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
US5712374A (en) 1995-06-07 1998-01-27 American Cyanamid Company Method for the preparation of substantiallly monomeric calicheamicin derivative/carrier conjugates
WO1997027757A1 (en) * 1996-01-31 1997-08-07 John Stephen Ayers Production of an immunoglobulin enriched fraction from whey protein solutions
US6037454A (en) 1996-11-27 2000-03-14 Genentech, Inc. Humanized anti-CD11a antibodies
US20020032315A1 (en) 1997-08-06 2002-03-14 Manuel Baca Anti-vegf antibodies
DE69907439T3 (en) * 1998-05-06 2014-01-02 Genentech, Inc. Purification of proteins by ion exchange chromatography
SE526227C2 (en) * 2002-05-15 2005-08-02 North China Pharmaceutical Group Method of purifying recombinant human serum albumin
RU2367667C2 (en) 2004-08-19 2009-09-20 Дженентек, Инк. Polypeptide variants with changed effector function
CA2580141C (en) 2004-09-23 2013-12-10 Genentech, Inc. Cysteine engineered antibodies and conjugates
US7867784B2 (en) 2004-10-21 2011-01-11 Ge Healthcare Bio-Science Ab Chromatography ligand
KR101482791B1 (en) * 2005-03-11 2015-01-21 와이어쓰 엘엘씨 A method of weak partitioning chromatography
NZ562949A (en) * 2005-04-11 2009-05-31 Medarex Inc Protein purification using HCIC and ion exchange chromatography
US7691980B2 (en) * 2007-01-09 2010-04-06 Bio-Rad Laboratories, Inc. Enhanced capacity and purification of antibodies by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
US9433922B2 (en) * 2007-08-14 2016-09-06 Emd Millipore Corporation Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same
KR101241486B1 (en) * 2007-10-30 2013-03-15 제넨테크, 인크. Antibody purification by cation exchange chromatography
JP5999899B2 (en) 2008-04-08 2016-09-28 バイオ−ラッド ラボラトリーズ インコーポレーティッド Chromatographic purification of antibodies
WO2010019148A1 (en) 2008-08-14 2010-02-18 Genentech, Inc. Methods for removing a contaminant using indigenous protein displacement ion exchange membrane chromatography
US8497358B2 (en) 2008-12-19 2013-07-30 Takeda Pharmaceutical Company Limited Antibody purification method
AU2010221099A1 (en) * 2009-03-06 2011-09-22 Medimmune, Llc Humanized anti-CD19 antibody formulations
EP2480561B1 (en) * 2009-09-23 2016-07-13 E. R. Squibb & Sons, L.L.C. Cation exchange chromatography

Also Published As

Publication number Publication date
PL2575847T5 (en) 2022-07-18
HK1246328A1 (en) 2018-09-07
EP2575847A4 (en) 2014-10-08
US20220064209A1 (en) 2022-03-03
HRP20171095T1 (en) 2017-10-06
IL261147A (en) 2018-10-31
ZA201208673B (en) 2016-02-24
CN102905718A (en) 2013-01-30
CN107365346A (en) 2017-11-21
US20200399307A1 (en) 2020-12-24
EP2575847A1 (en) 2013-04-10
CA2799915A1 (en) 2011-12-01
MX370828B (en) 2020-01-08
SI2575847T2 (en) 2022-08-31
ES2637613T5 (en) 2022-07-20
MY161534A (en) 2017-04-28
WO2011150110A1 (en) 2011-12-01
EP2575847B1 (en) 2017-06-14
AU2011258254B2 (en) 2016-01-07
CA2799915C (en) 2023-09-26
EP2575847B2 (en) 2022-04-27
AU2011258254A1 (en) 2013-01-10
MX344248B (en) 2016-12-09
JP2013530156A (en) 2013-07-25
KR101976853B1 (en) 2019-05-09
JP2018087192A (en) 2018-06-07
IL223029A0 (en) 2013-02-03
RU2594163C2 (en) 2016-08-10
US20130079272A1 (en) 2013-03-28
JP6272941B2 (en) 2018-01-31
MX2012013575A (en) 2013-03-21
ES2637613T3 (en) 2017-10-13
JP2016190851A (en) 2016-11-10
SI2575847T1 (en) 2017-09-29
RU2012156271A (en) 2014-06-27
CA3209878A1 (en) 2011-12-01
NZ603648A (en) 2014-12-24
US20190300566A1 (en) 2019-10-03
SG185737A1 (en) 2013-01-30
EP3299380A1 (en) 2018-03-28
KR20130086544A (en) 2013-08-02
JP5934190B2 (en) 2016-06-15
HRP20171095T4 (en) 2022-07-22
PL2575847T3 (en) 2017-10-31
BR112012029904A2 (en) 2017-08-08

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