SG10201701224UA - Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode - Google Patents

Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode

Info

Publication number
SG10201701224UA
SG10201701224UA SG10201701224UA SG10201701224UA SG10201701224UA SG 10201701224U A SG10201701224U A SG 10201701224UA SG 10201701224U A SG10201701224U A SG 10201701224UA SG 10201701224U A SG10201701224U A SG 10201701224UA SG 10201701224U A SG10201701224U A SG 10201701224UA
Authority
SG
Singapore
Prior art keywords
removal
protein aggregates
flowthrough mode
biopharmaceutical preparations
biopharmaceutical
Prior art date
Application number
SG10201701224UA
Inventor
Mikhail Kozlov
William Cataldo
Ajish Potty
Kevin Galipeau
James Hamzik
Lars Peeck
Joaquin Umana
Original Assignee
Merck Patent Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent Gmbh filed Critical Merck Patent Gmbh
Publication of SG10201701224UA publication Critical patent/SG10201701224UA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
    • C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
    • C08F220/52—Amides or imides
    • C08F220/54—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide
    • C08F220/58—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide containing oxygen in addition to the carbonamido oxygen, e.g. N-methylolacrylamide, N-(meth)acryloylmorpholine
    • C08F220/585—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide containing oxygen in addition to the carbonamido oxygen, e.g. N-methylolacrylamide, N-(meth)acryloylmorpholine and containing other heteroatoms, e.g. 2-acrylamido-2-methylpropane sulfonic acid [AMPS]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/08—Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/16—Organic material
    • B01J39/18—Macromolecular compounds
    • B01J39/20—Macromolecular compounds obtained by reactions only involving unsaturated carbon-to-carbon bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/26—Cation exchangers for chromatographic processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J47/00—Ion-exchange processes in general; Apparatus therefor
    • B01J47/02—Column or bed processes
    • B01J47/026—Column or bed processes using columns or beds of different ion exchange materials in series
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
    • C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
    • C08F220/52—Amides or imides
    • C08F220/54—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide
    • C08F220/56—Acrylamide; Methacrylamide
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F222/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a carboxyl radical and containing at least one other carboxyl radical in the molecule; Salts, anhydrides, esters, amides, imides, or nitriles thereof
    • C08F222/36—Amides or imides
    • C08F222/38—Amides
    • C08F222/385—Monomers containing two or more (meth)acrylamide groups, e.g. N,N'-methylenebisacrylamide
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Polymers & Plastics (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Graft Or Block Polymers (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
SG10201701224UA 2012-03-12 2013-01-09 Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode SG10201701224UA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261609533P 2012-03-12 2012-03-12
US201261666578P 2012-06-29 2012-06-29

Publications (1)

Publication Number Publication Date
SG10201701224UA true SG10201701224UA (en) 2017-04-27

Family

ID=48044702

Family Applications (1)

Application Number Title Priority Date Filing Date
SG10201701224UA SG10201701224UA (en) 2012-03-12 2013-01-09 Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode

Country Status (10)

Country Link
US (2) US20130245139A1 (en)
EP (3) EP3730510A1 (en)
JP (3) JP5768070B2 (en)
KR (4) KR101520753B1 (en)
CN (3) CN103382215B (en)
ES (2) ES2644744T3 (en)
PL (1) PL2639239T3 (en)
SG (1) SG10201701224UA (en)
SI (1) SI2639239T1 (en)
WO (1) WO2013138098A1 (en)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101997543B1 (en) 2010-07-30 2019-07-09 이엠디 밀리포어 코포레이션 Chromatogrphy media and method
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
US9096648B2 (en) * 2011-08-19 2015-08-04 Emd Millipore Corporation Methods of reducing level of one or more impurities in a sample during protein purification
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
US9150645B2 (en) 2012-04-20 2015-10-06 Abbvie, Inc. Cell culture methods to reduce acidic species
EP2682168A1 (en) * 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
ES2868093T3 (en) 2012-09-17 2021-10-21 Grace W R & Co Chromatography media and devices
WO2014143205A1 (en) 2013-03-12 2014-09-18 Abbvie Inc. Human antibodies that bind human tnf-alpha and methods of preparing the same
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
US9499614B2 (en) 2013-03-14 2016-11-22 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides
DK2969099T4 (en) * 2013-03-14 2022-03-14 Amgen Inc REMOVAL OF LEAKED AFFINITY PURIFICATION LIGAND
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
WO2015073884A2 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
SG10201804200WA (en) 2013-12-12 2018-07-30 Emd Millipore Corp Protein separations using an acrylamide containing filter
EP3094390B1 (en) 2014-01-16 2021-07-07 W.R. Grace & CO. - CONN. Affinity chromatography media and chromatography devices
ES2756601T3 (en) * 2014-01-31 2020-04-27 Enginzyme Ab Immobilized proteins and their use
US11389783B2 (en) 2014-05-02 2022-07-19 W.R. Grace & Co.-Conn. Functionalized support material and methods of making and using functionalized support material
WO2016013609A1 (en) * 2014-07-25 2016-01-28 旭化成メディカル株式会社 Carrier for positive-ion-exchange chromatography and method for using same
WO2016036508A1 (en) * 2014-09-02 2016-03-10 Emd Millipore Corporation High surface area fiber media with nano-fibrillated surface features
JPWO2016093251A1 (en) * 2014-12-08 2017-05-25 旭化成メディカル株式会社 Method for purifying physiologically active substance
JP6665184B2 (en) 2014-12-08 2020-03-13 イー・エム・デイー・ミリポア・コーポレイシヨン Mixed bed ion exchange adsorbent
JP2016210705A (en) * 2015-04-30 2016-12-15 昭和電工株式会社 Method of removing protein aggregates
US10695744B2 (en) 2015-06-05 2020-06-30 W. R. Grace & Co.-Conn. Adsorbent biprocessing clarification agents and methods of making and using the same
RU2018121653A (en) * 2015-11-18 2019-12-19 Мерк Патент Гмбх IMPROVED PROTEIN SEPARATION IN ION EXCHANGE CHROMATOGRAPHY
CN106807330A (en) * 2015-11-27 2017-06-09 中国科学院大连化学物理研究所 The preparation and sorbing material and application of a kind of ordered structure sorbing material
US20190023736A1 (en) * 2016-01-22 2019-01-24 Asahi Kasei Medical Co., Ltd. Method for purifying protein
JP7032046B2 (en) * 2016-01-22 2022-03-08 旭化成メディカル株式会社 Continuous constant flow rate purification method for bioactive substances
JP6621176B2 (en) * 2016-01-22 2019-12-18 旭化成メディカル株式会社 Protein purification method
EP3275897A1 (en) * 2016-07-27 2018-01-31 Biotest AG Process for preparing immunoglobulin compositions
JP7063811B2 (en) * 2016-08-31 2022-05-09 協和キリン株式会社 Protein purification method using activated carbon
EP3510041B1 (en) * 2016-09-09 2023-05-31 3M Innovative Properties Company Processes for separating aggregated proteins from monomeric proteins in a biological solution
EP3511710B1 (en) 2016-09-09 2021-03-03 Asahi Kasei Medical Co., Ltd. Strong cation exchange chromatography carrier and use of same
US11446613B2 (en) 2016-09-09 2022-09-20 3M Innovative Properties Company Functionalized copolymers and use thereof
EP3538241A1 (en) * 2016-11-10 2019-09-18 Sandoz AG Process for desalting of a protein solution
WO2019169040A1 (en) 2018-02-27 2019-09-06 Life Technologies Corporation Flocculant functionalized separation media
WO2019173731A1 (en) * 2018-03-08 2019-09-12 Bio-Rad Laboratories, Inc. Anionic exchange-hydrophobic mixed mode chromatography resin
EP3546475A1 (en) * 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
WO2019192877A1 (en) 2018-04-03 2019-10-10 Merck Patent Gmbh Cex chromatography media and low salt elution of target proteins from biopharmaceutical feeds
US11045773B2 (en) * 2018-08-31 2021-06-29 Pall Corporation Salt tolerant porous medium
DE102018009597A1 (en) * 2018-12-07 2020-06-10 Sartorius Stedim Biotech Gmbh Device and method for multiple changes in the composition of a fluid
CN113302197A (en) * 2019-01-23 2021-08-24 第一三共株式会社 Method for purifying antibody including process using activated carbon material
US10981950B2 (en) 2019-01-31 2021-04-20 Joanna Pezzini Therapeutic protein flow kit for a continuous purification system
CN114040815A (en) 2019-09-05 2022-02-11 生物辐射实验室股份有限公司 Anion exchange-hydrophobic mixed mode chromatography resin
CN114729003A (en) 2019-11-22 2022-07-08 莫佛塞斯公司 Method for increasing antibody yield in ion exchange chromatography process
CN115397834A (en) * 2020-04-13 2022-11-25 3M创新有限公司 Flow-through method and apparatus for purifying target molecules
WO2021232009A1 (en) * 2020-05-15 2021-11-18 Stevenson Gary L Multistage oil reclamation system
CN113416235B (en) * 2021-06-24 2023-04-25 苏州赛分科技股份有限公司 Liquid chromatography for purifying and separating virus antigens
CN113522256B (en) * 2021-07-19 2022-06-21 中国科学院兰州化学物理研究所 Preparation and application of hydrogel @ silicon dioxide liquid chromatography filler
KR20240046733A (en) 2021-08-23 2024-04-09 제넨테크, 인크. Perfusion Cation Exchange Chromatography Purification Process for Antibody Drug Conjugates
US20230129803A1 (en) * 2021-10-27 2023-04-27 Plasma Technologies, Llc Compositions and methods for isolating proteins

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4618533A (en) 1984-11-30 1986-10-21 Millipore Corporation Porous membrane having hydrophilic surface and process
US4944879A (en) 1989-07-27 1990-07-31 Millipore Corporation Membrane having hydrophilic surface
US6451260B1 (en) * 1997-08-26 2002-09-17 Dyax Corp. Method for producing microporous elements, the microporous elements thus produced and uses thereof
ATE274522T1 (en) 1998-06-01 2004-09-15 Genentech Inc SEPARATION OF ANTIBODY MONOMERS FROM THEIR MULTIMERS USING ION EXCHANGE CHROMATOGRAPHY
US6783937B1 (en) * 1999-02-25 2004-08-31 Pall Corporation Negatively charged membrane
US6783713B2 (en) * 1999-05-03 2004-08-31 The Procter & Gamble Co. Filters for removal of pathogens from liquids
AUPQ389599A0 (en) * 1999-11-05 1999-12-02 Ilion Technology Corporation Polyelectrolyte gel
JP4918952B2 (en) * 2001-03-28 2012-04-18 Ｄｉｃ株式会社 Water-absorbing material
US7318972B2 (en) * 2001-09-07 2008-01-15 Itm Power Ltd. Hydrophilic polymers and their use in electrochemical cells
AU2003212912A1 (en) 2002-02-04 2003-09-02 Millipore Corporation Process for removing protein aggregates and virus from a protein solution
AU2004212641B2 (en) * 2003-02-19 2009-08-06 Merck Millipore Ltd. Composite materials comprising supported porous gels
JPWO2004087761A1 (en) * 2003-03-31 2006-07-27 麒麟麦酒株式会社 Purification of human monoclonal antibodies and human polyclonal antibodies
AU2004285928B2 (en) 2003-10-24 2012-02-02 Amgen, Inc. Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction
EP2336172B1 (en) * 2003-10-27 2014-12-10 Wyeth LLC Removal of high molecular weight aggregates using hydroxyapatite chromatography
SE0302911D0 (en) * 2003-10-31 2003-10-31 Amersham Biosciences Ab Novel separation matrix
US7867784B2 (en) * 2004-10-21 2011-01-11 Ge Healthcare Bio-Science Ab Chromatography ligand
US20070012345A1 (en) * 2005-01-24 2007-01-18 Owens Tony L Lower limb-support ambulatory device
KR101482791B1 (en) 2005-03-11 2015-01-21 와이어쓰 엘엘씨 A method of weak partitioning chromatography
ATE534444T1 (en) * 2005-07-28 2011-12-15 Bio Rad Laboratories SEPARATION OF PROTEINS ON THE BASIS OF ISOELECTRIC POINT USING SOLID PHASE BUFFERS
US7674836B2 (en) * 2006-07-28 2010-03-09 3M Innovative Properties Company Method of making macroporous cation exchange resins
DK2061803T4 (en) * 2006-08-28 2022-12-05 Ares Trading Sa PROCEDURE FOR PURIFICATION OF FC-CONTAINING PROTEINS
DE102006061327A1 (en) * 2006-12-22 2008-06-26 Basf Construction Polymers Gmbh Grafting copolymer, useful e.g. as additives in construction chemical applications, water retention agents and fluid loss additives, comprises silica, which is reacted with an unsaturated silane and a polymer containing sulfonic acid
WO2008085116A1 (en) * 2007-01-10 2008-07-17 Ge Healthcare Bio-Sciences Ab Mult i -modal ion exchange chromotography resins
DE102007012786A1 (en) * 2007-03-16 2009-01-08 Construction Research & Technology Gmbh Preparation of sulfo-containing copolymers
US20080257814A1 (en) 2007-04-23 2008-10-23 Millipore Corporation Filtration Cartridge
EP2152405B2 (en) * 2007-05-25 2017-04-05 Merck Patent GmbH Graft copolymer for cation-exchange chromatography
JP5064558B2 (en) * 2007-06-01 2012-10-31 エフ．ホフマン−ラ ロシュ アーゲー Immunoglobulin purification
CN101868293B (en) 2007-10-03 2013-07-03 Emd密理博公司 Stacked plates filtration cartridge
JP5132278B2 (en) * 2007-11-28 2013-01-30 一般財団法人川村理化学研究所 Method for producing organic-inorganic composite hydrogel
WO2009105191A2 (en) 2008-02-19 2009-08-27 Bloom Energy Corporation Fuel cell system containing anode tail gas oxidizer and hybrid heat exchanger/reformer
EP2153877A1 (en) * 2008-07-30 2010-02-17 MERCK PATENT GmbH Mixed graft polymers for ion exchange chromatography
AU2008221604B2 (en) * 2008-09-22 2010-04-22 Commonwealth Scientific And Industrial Research Organisation Temperature-responsive polymer particles in protein separation applications
EP2401065B1 (en) * 2009-02-27 2017-12-13 EMD Millipore Corporation Membrane with sulfonic groups for removing protein aggregates
KR101729797B1 (en) * 2009-05-20 2017-04-24 바스프 에스이 Hydrophobically associating copolymers
CN107188960A (en) * 2009-08-07 2017-09-22 Emd密理博公司 The method of target protein from one or more impurity of sample
WO2011156073A1 (en) * 2010-06-08 2011-12-15 Millipore Corporation Removal of protein aggregates from biopharmaceutical preparations using calcium phosphate salts
GB201012603D0 (en) * 2010-07-27 2010-09-08 Ucb Pharma Sa Protein purification
KR20130028939A (en) * 2010-07-29 2013-03-20 이엠디 밀리포어 코포레이션 Grafting method to improve chromatography media performance
KR101997543B1 (en) * 2010-07-30 2019-07-09 이엠디 밀리포어 코포레이션 Chromatogrphy media and method

Also Published As

Publication number Publication date
ES2812648T3 (en) 2021-03-17
JP6580650B2 (en) 2019-09-25
SI2639239T1 (en) 2017-12-29
CN103382215A (en) 2013-11-06
KR101520753B1 (en) 2015-05-15
JP2015110786A (en) 2015-06-18
ES2644744T3 (en) 2017-11-30
JP2018048162A (en) 2018-03-29
CN104817611A (en) 2015-08-05
JP6231027B2 (en) 2017-11-15
KR20130105340A (en) 2013-09-25
CN103382215B (en) 2016-08-24
EP3312190A1 (en) 2018-04-25
KR101601812B1 (en) 2016-03-10
KR101966692B1 (en) 2019-04-09
EP2639239A3 (en) 2013-11-20
US20130245139A1 (en) 2013-09-19
KR20150093642A (en) 2015-08-18
EP2639239B1 (en) 2017-09-06
EP3312190B1 (en) 2020-05-27
JP2013189427A (en) 2013-09-26
CN110256628A (en) 2019-09-20
JP5768070B2 (en) 2015-08-26
KR20150043271A (en) 2015-04-22
KR20140141562A (en) 2014-12-10
EP2639239A2 (en) 2013-09-18
EP3730510A1 (en) 2020-10-28
WO2013138098A1 (en) 2013-09-19
PL2639239T3 (en) 2018-03-30
US20180215786A1 (en) 2018-08-02
CN104817611B (en) 2019-06-18

Similar Documents

Publication Publication Date Title
SG10201701224UA (en) Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode
IL269565A (en) Plasma kallikrein binding proteins
IL276534A (en) Methods for purifying serpin containing fusion proteins
HRP20181357T1 (en) Novel protein purification methods
IL230918A0 (en) Modified proteins and peptides
HK1206370A1 (en) Charged nutritive proteins and methods
EP2809811A4 (en) Modification of dna on magnetic beads
EP2883882A4 (en) Protein purification method
EP2900697A4 (en) Peptide directed protein knockdown
IL239285A0 (en) High protein yogurts
ZA201400562B (en) Axmi205 variant proteins and methods for their use
PT2858490T (en) Recombinant dna elements for the expression of recombinant proteins in insects
SG11201505202SA (en) Materials and methods for removing endotoxins from protein preparations
EP2828975A4 (en) Configurable multi-mode media independent interface
EP2683742A4 (en) Npp1 fusion proteins
EP2744820A4 (en) Methods for extracting keratin proteins
HK1212995A1 (en) A method of purifying proteins
EP2701735A4 (en) Liquid vaccine preparations
HK1203404A1 (en) Serum amyloid p-antibody fusion proteins p-
EP2829603A4 (en) Protein alignment method
EP2771689A4 (en) Usp2a peptides and antibodies
GB201213672D0 (en) Protein
GB201121236D0 (en) Proteins and peptides
GB201215901D0 (en) Protein modification
GB201217405D0 (en) Protein removal agent