RU2641924C1 - Sorption material, method of its production and method of its application - Google Patents

Abstract

FIELD: chemistry.

SUBSTANCE: claimed sorption material contains a porous carrier, the functional groups on the surface of which are covalently bound to a ligand capable of forming strong complexes with bacterial endotoxins. The porous carrier is a pellet with a size of 50 to 900 microns, made of a polymer or a copolymer. The carrier is obtained on the basis of such monomers as acrylic acid, methacrylic acid, acrylamide, metakrilamid, methyl acrylate, methyl methacrylate, glicidil methacrylate, vinyl acetate, allilamin, sodium 2-metilprop-2-en-1-sulfonate, allylglycidyl ether, divinylbenzene, divinylbenzene, ethylene glycol, triethylene glycol dimetakrilat, N,N-bis (metakrilamid). The ligand for binding bacterial endotoxins is an amphiphilic organic compound containing primary and secondary amino groups and hydrophobic substituents. A method for the preparation of a new sorption material and its use for purifying an aqueous solution of protein or an aqueous saline solution or a solution of a blood plasma are proposed.

EFFECT: obtaining new selective sorbents for purification of liquid media from bacterial endotoxins.

3 cl, 2 tbl, 15 ex

Description

Technical field

The invention relates to the field of new sorption materials designed to remove bacterial endotoxins from aqueous solutions, including from aqueous solutions of proteins, and organic compounds containing inorganic salts.

State of the art

Bacterial endotoxins are pyrogenic substances, already in very small doses, greatly increase the body temperature of warm-blooded animals when ingested. If bacterial endotoxins directly enter the bloodstream, for example as a result of an intravenous injection of a drug containing them as an impurity, then this will lead to intense heat and fever. If the similar pyrogenic effect of bacterial endotoxins getting into the blood becomes too strong, the fever will be accompanied by chills, trembling, can go into a shock state and lead to death.

Although substances of various structures have a pyrogenic effect, bacterial endotoxins are the most important and most active pyrogens. It is lipopolysaccharides, fragments of the cell wall of gram-negative bacteria that have the most pronounced pyrogenic effect. Even diluted solutions, such as concentrations of ~ 10 ^-12 g / l, have physiological activity. Lipopolysaccharides are surface-active substances and in aqueous solutions exist in the form of aggregates of variable composition and structure. Such aggregates may include other components of the solution, so if a substance is contaminated with lipopolysaccharides, it is very difficult to clear it.

To date, various methods are used to remove bacterial endotoxins:

(1) adsorption using activated carbon or ion exchange resins,

(2) hydrolytic cleavage of bacterial endotoxins with acids or alkalis,

(3) the oxidative decomposition of bacterial endotoxins using aqueous solutions of hydrogen peroxide, potassium permanganate, sodium hypochlorite and the like,

(4) membrane microfiltration,

other similar methods.

One way or another, the complete removal of bacterial endotoxins using these methods in one operation is difficult or impossible.

In addition, these methods have significant drawbacks. For example, a pharmaceutical substance that is purified from bacterial endotoxin impurities using method (1) is also sorbed, which leads to its loss, or is also destroyed using methods (2) and (3).

A number of patent documents are known related to the sorption removal of pyrogens and endotoxins from liquid media, for example:

DE 4113602 (A1) Highly selective endotoxin adsorber - consists of bead-like water swollen cellulose prod, contg. polyethylene-imine as the functional ligand (IPC B01D 15/00; B01J 20/22; B01J 20/28; B01J 20/32; A61M 1/36; (IPC1-7): A61M 1/36; B01D 15/00; B01J 20/26; B01J 20/28; B01J 20/30; C02F 1/28, publ. 1992-10-29);

US 5,279,821 (A) Pyrogen adsorbent containing amide groups (IPC A61K 31/74; A61K 9/16; (IPC1-7): A61K 31/785; A61K 37/02; A61L 2/16; C08G 69/08; C08G 69 / 48, publ. 1994-01-18);

US 5547576 (A) Pathogenic substance removing material and a blood filter containing the material (IPC A61L 2/00; A61L 2/02; A61M 1/36; B01D 39/16; B01D 67/00; (IPC1-7): B01D 63/00; B01D 69/12; B01D 71/60, publ. 1996-08-20);

WO 9104086 (A1) FILTER MEDIA AND USE FOR PYROGEN REMOVAL (IPC B01D 15/00; B01J 20/32; (IPC1-7): B01D 15/08, published 1991-04-04);

WO 0016897 (A1) MICROFILTRATION FILTER LAYER FOR SEPARATING ENDOTOXINS AND THE USE OF SAID MICROFILTRATION FILTER LAYER (IPC C07K 1/34; (IPC1-7): B01D 15/00; B01D 61/00; B01J 20/32, publ. 2000 -03-30);

US 4381239 (A) Method for reducing the pyrogen content of or removing pyrogens from substances contaminated therewith (IPC A61K 9/00; A61K 9/08; A61K 9/18; B01D 15/00; B01D 15/08; B01J 20/26 ; B01J 20/32; C08B 11/14; C08B 37/00; C08B 37/12; (IPC1-7): B01D 15/00, publ. 1983-04-26);

US 6106723 (A) Method for removing pyrogens from dialysate (IPC B01D 61/00; B01D 67/00; B01D 69/14; B01J 20/28; B01J 20/32; C02F 1/44, publ. 2000-08-22 );

RU 2426557, SORPTION-BACTERICIDAL MATERIAL, METHOD FOR ITS PREPARATION, METHOD FOR FILTING LIQUID OR GAS MEDIA, MEDICAL SORBENT (IPC A61L 15/18, A61F 13/00, A61K 9/70, 2011 Vl. there is a PCT application WO 2011071417 (A1) BACTERICIDAL SORBENT MATERIAL AND METHOD FOR PRODUCING SAME (publ. 2011-06-16).

After a thorough analysis of all these inventions, the closest in technical essence and the achieved result to the claimed solution, according to the developers, the invention is recognized as SORPTION-BACTERICIDAL MATERIAL, METHOD FOR ITS PREPARATION, METHOD FOR FILTING LIQUID OR GAS-MEDIA, MEDICAL. The sorption-bactericidal material contains a non-woven polymeric fibrous material with highly porous particles of aluminum oxide hydrate fixed to its fibers, while the inorganic bactericidal component is sorbed on highly porous particles of aluminum oxide hydrate. A method for producing a sorption-bactericidal material includes treating the material with a solution of an inorganic bactericidal component and treating a non-woven polymeric fibrous material on the fibers of which highly porous particles of aluminum oxide hydrate are fixed for a period not exceeding 24 hours. A method for filtering liquid or gaseous media involves passing a liquid or gaseous medium through sorption and bactericidal material. The proposed sorption-bactericidal material based on a fibrous matrix and inorganic particles and ions has bactericidal activity and the ability to remove bacterial endotoxins. The mechanism of action is explained by non-selective adsorption on the pores of the fibers and the formation of bridge coordination structures of oxygen-containing functional groups of the removed organic compounds with metal ions and particles fixed to the sorbent fibers.

The low pore volume and the absence in the composition of this material of structural fragments having a specific affinity for bacterial endotoxins allow us to attribute the low sorption capacity, low activity and lack of selectivity to bacterial endotoxins to the disadvantages of this invention. Thus, it is obvious that there is a need to create fundamentally new materials capable of selectively removing bacterial endotoxins from solutions of organic compounds of complex composition and structure, including physiologically active proteins.

Disclosure of invention

The technical problem to which the present invention is directed was the creation of a new sorption material with improved sorption activity and selectivity with respect to the bound and removed from the solution endotoxins in the presence of excess organic compounds of variable structure and composition in these solutions.

Another technical challenge facing the developers was the development of a new method for producing amphiphilic organic molecules containing primary and secondary amino groups and hydrophobic substituents, which are used as immobilized ligands for binding bacterial endotoxins.

Another technical challenge facing the developers was the development of a new, less long and laborious method for producing porous polymer granules containing reactive functional groups on the surface.

And finally, the last technical task of the invention was to develop a method of filtering (purification) of liquid media, including ensuring contact of liquid media with the proposed sorption material.

Due to these circumstances, selective sorbents that selectively bind bacterial endotoxins have been studied and proposed. Such sorbents are water-insoluble porous polymers, on the surface of which amphiphilic organic compounds are covalently immobilized, having a high affinity for bacterial endotoxins, containing primary and secondary amino groups and hydrophobic substituents. Methods are proposed for using such sorbents to reduce or completely remove bacterial endotoxins from solutions containing proteins and small molecules, inorganic salts, without significantly changing the composition of such solutions.

The present invention relates to a sorbent, which is a porous polymeric material consisting of such monomers as acrylic acid, methacrylic acid, acrylamide, methacrylamide, methyl acrylate, methyl methacrylate, glycidyl methacrylate, vinyl acetate, allylamine, sodium 2-methylprop-2- en-1-sulfonate, allyl glycidyl ether, divinylbenzene, ethylene glycol dimethacrylate, triethylene glycol dimethacrylate, N, N-bis (methacrylamide), and a ligand of the general formula

which is an amphiphilic organic molecule, which is connected by several covalent bonds with functional groups on the surface of a water-insoluble porous polymer material. The sorbent has a high capacity and selectivity, acceptable mechanical properties, is simple and cheap to manufacture, can withstand sterilization.

The sorption material is a porous polymer carrier, the functional groups on the surface of which are covalently linked to the ligand in an amount of 1% to 25% by weight, capable of forming strong complexes with bacterial endotoxins. A porous polymeric carrier in which the base material is granules from 50 to 900 microns in size of a polymer or copolymer consisting of monomers such as acrylic acid, methacrylic acid, acrylamide, methacrylamide, methyl acrylate, methyl methacrylate, glycidyl methacrylate, vinyl acetate, allylamine , sodium 2-methylprop-2-en-1-sulfonate, allyl glycidyl ether, divinylbenzene, ethylene glycol dimethacrylate, triethylene glycol dimethacrylate, N, N-bis (methacrylamide). The bacterial endotoxin binding ligand is an amphiphilic organic compound containing primary and secondary amino groups and hydrophobic substituents, and is indicated by the following formula

where R is an aliphatic unbranched radical C _n H _{2n + 1} , where n is in the range from 1 to 18; benzyl; 4-chlorobenzyl; 4-phenylbenzyl; 4-methylbenzyl;

the ratio of x + y lies in the range from 1 to 4;

the ratio of y to x lies in the range from 0 to 1.

A method of producing sorption material consists in polymerizing the following components or mixtures thereof: acrylic acid, methacrylic acid, acrylamide, methacrylamide, methyl acrylate, methyl methacrylate, glycidyl methacrylate, vinyl acetate, allylamine, sodium 2-methylprop-2-en-1-sulfonate, allyl glycidyl ether, divinylbenzene, ethylene glycol dimethacrylate, triethylene glycol dimethacrylate, N, N-bis (methacrylamide). The polymerization is carried out in the presence of cyclohexanol, toluene, polyvinyl alcohol, polyvinylpyrrolidone or mixtures thereof at a temperature of 70-80 ° C. The ligand synthesis is carried out separately using one of the following components: ethylene diamine, diethylene triamine, triethylenetetramine or tetraethylene pentamine, which are mixed with one of the following components: octadecyl bromide, dodecyl bromide, octyl bromide, butyl bromide, methyl iodide, benzyl chloride, 4-chlorobenz 4-methylbenzyl chloride, 4-phenylbenzyl chloride. The reaction is carried out in ethanol as a solvent by boiling. Then the ligand is immobilized on a polymeric carrier at room temperature or boiling in ethanol.

The invention also relates to the field of application of the sorbent for the purification of organic compounds, both protein and small molecules, in particular, physiologically active substances and mixtures thereof. Specifically, a method of using the sorption material is to purify an aqueous protein solution, or an aqueous saline solution, or a blood plasma solution, according to which 50-100 ml of a contaminated solution is placed in a vessel containing 10-40 ml of sorbent at a temperature of 20-37 ° C time from one minute to three hours, after which the solution is separated by filtration.

Examples of carrying out the invention

Example 1. The synthesis of ligands

100 ml of ethylenediamine, 128 ml of octadecyl bromide and 1 l of ethanol are placed in a 2 L round-bottom flask. The reaction is carried out by boiling for 24 hours. The resulting compound (ligand A01-C18-50) is used without isolation and purification.

Similarly received:

ligand A01-C 12-50, based on 100 ml of ethylenediamine and 90 ml of dodecyl bromide;

ligand A01-C8-50, based on 100 ml of ethylenediamine and 65 ml of octyl bromide;

ligand A01-C4-50, starting from 100 ml of ethylenediamine and 40 ml of butyl bromide;

ligand A01-C1-50, starting from 100 ml of ethylenediamine and 23 ml of methyl iodide;

ligand A02-C8-50, based on 100 ml of diethylene triamine and 40 ml of octyl bromide;

ligand A02-C8-100, based on 100 ml of diethylene triamine and 80 ml of octyl bromide;

ligand A03-C8-50, based on 100 ml of triethylenetetramine and 29 ml of octyl bromide;

ligand A04-C8-50, based on 100 ml of tetraethylene pentamine and 23 ml of octyl bromide;

ligand A02-B-25, based on 100 ml of diethylene triamine and 13 ml of benzyl chloride;

ligand A02-X-25, based on 100 ml of diethylene triamine and 19 g of 4-chlorobenzyl chloride;

ligand A02-M-25, starting from 100 ml of diethylene triamine and 15 ml of 4-methylbenzyl chloride;

ligand A03-DF-25, starting from 100 ml of diethylene triamine and 23 g of 4-phenylbenzyl chloride.

Example 2. The synthesis of microspheres

Glycidyl methacrylate (102 ml), triethylene glycol dimethacrylate (22 ml), toluene (150 ml), polyvinyl alcohol (9.5 g) and distilled water (900 ml) are charged into a 5 L reactor equipped with a frame stirrer. Stirred at room temperature at a speed of 500 rpm for 15 minutes Then, azobisisobutyronitrile (1.3 g) was added. The reaction is carried out at a stirring speed of 300 rpm and a temperature of 70 ° C for 2 hours, then at a temperature of 80 ° C for 4 hours. The contents are discharged from the reactor onto a glass filter with a porosity of 3, washed with water (10 times 1 l), then methanol (3 times 350 ml). Dried in the air. Then sieving is carried out on a vibrating screen with the separation of a fraction of particles with a diameter of 100-500 microns (P01).

Similarly, microgranules were obtained:

P02, starting from allyl glycidyl ether (31 ml), ethylene glycol dimethacrylate (70 ml), cyclohexanol (100 ml), polyvinylpyrrolidone-8000 (12 g) and azobisisobutyronitrile (1.0 g);

P03, starting from sodium 2-methylprop-2-en-1-sulfonate (1.0 g), methyl methacrylate (110 ml), divinylbenzene (3.0 ml, 80%), toluene (30 ml), polyvinylpyrrolidone-8000 (9.0 g) and azobisisobutyronitrile (1.1 g);

P04, based on sodium 2-methylprop-2-en-1-sulfonate (14 g), methyl methacrylate (100 ml), allylamine (6.0 ml), divinylbenzene (6.0 ml, 80%), toluene (60 ml), polyvinylpyrrolidone-8000 (9.0 g) and azobisisobutyronitrile (1.2 g);

P05, based on acrylic acid (20 ml), methyl acrylate (50 ml), methyl methacrylate (50 ml), vinyl acetate (6.0 ml), N, N-bis (methacrylamide) (12 g), cyclohexanol (100 ml), polyvinyl alcohol (9.0 g) and azobisisobutyronitrile (1.3 g);

P06, based on methacrylic acid (14 ml), acrylamide (7.0 ml), methacrylamide (7.0 ml), methyl methacrylate (80 ml), N, N-bis (methacrylamide) (11 g), cyclohexanol (50 ml), toluene (50 ml), polyvinyl alcohol (10 g) and azobisisobutyronitrile (1.2 g).

Example 3. Synthesis of sorbents M01-14

A portion of P01 microbeads (50 g) is placed in a 2 L flask; A01-C18-50 ligand solution (500 ml) and ethanol (500 ml) are added. The reaction is carried out under boiling for 24 hours. Then the sorbent (M01) is transferred to a glass filter with a porosity of 3 and washed successively with ethanol (3 times 500 ml), distilled water (3 times 500 ml) and ethanol (3 times 150 ml) . Store under a layer of ethanol (100 ml per 10 ml of sorbent).

Similarly, sorbents were obtained:

M02, based on P01 microgranules (50 g) and A01-C12-50 ligand (500 ml);

M03, based on P01 microspheres (50 g) and A01-C8-50 ligand (500 ml);

M04, based on P01 microgranules (50 g) and A01-C4-50 ligand (500 ml);

M05, based on P01 microgranules (50 g) and A01-C1-50 ligand (500 ml);

M06, based on P01 microspheres (50 g) and A02-C8-50 ligand (500 ml);

M07, based on P01 microgranules (50 g) and A02-C8-100 ligand (500 ml);

M08, based on P01 microgranules (50 g) and A03-C8-50 ligand (500 ml);

M09, based on P01 microgranules (50 g) and A04-C8-50 ligand (500 ml);

M10, based on P01 microgranules (50 g) and A02-B-25 ligand (500 ml);

M11, based on P01 microgranules (50 g) and A02-X-25 ligand (500 ml);

M12, starting from P01 microspheres (50 g) and A02-M-25 ligand (500 ml);

M13, based on P01 microgranules (50 g) and ligand A03-DF-25 (500 ml);

M14, based on P02 microgranules (50 g) and A02-C8-50 ligand (500 ml).

Example 4. The synthesis of sorbents M15-20

A portion of P03 microspheres (50 g) is placed in a 2 L flask, a solution of A02-C8-50 ligand (300 ml) and ethanol (700 ml) are added. The reaction is carried out at room temperature for 12 hours. Then the sorbent (M15) is transferred to a glass filter with a porosity of 3 and washed successively with ethanol (3 times 500 ml), distilled water (3 times 500 ml) and ethanol (3 times 150 ml) ) Store under a layer of ethanol (100 ml per 10 ml of sorbent).

Similarly, sorbents were obtained:

M16, based on P03 microspheres (50 g) and A02-C8-100 ligand (500 ml);

M17, based on P03 microspheres (50 g) and A02-B-25 ligand (500 ml);

M18, based on microspheres P04 (50 g) and ligand A02-C8-50 (500 ml);

M19, based on microspheres P05 (50 g) and ligand A02-C8-50 (500 ml);

M20, based on P06 microspheres (50 g) and A02-C8-50 ligand (500 ml).

Example 5. Depyrogenation of an aqueous protein solution

Sorbent preparation. Sorbent (50 ml) was transferred to a glass filter, separated from ethanol, washed with pyrogen-free water (10 times 50 ml), then 0.5 M NaOH (1 time, 50 ml), saline (50 ml each to pH <9) and pyrogen-free water (1 time, 50 ml).

A solution of bovine serum albumin (1 mg / ml) in phosphate buffered saline (100 ml, 0.1 M, pH 7.0) contaminated with bacterial endotoxin (30 U / ml, E. coli O113: H10) was placed in a depyrogenated flask volume of 250 ml. Then, a sorbent in an amount of 40 ml was added to the solution. The mixture was incubated at 37 ± 2 ° C for 3 hours. Sampling was carried out after 1, 5, 30, 60 and 180 minutes.

The determination of bacterial endotoxin was carried out by the endogenous chromogenic LAL test. A biuret method was used to evaluate protein content.

Example 6. Depyrogenation of an aqueous protein solution

Preparation of the sorbent and purification of the aqueous protein solution was carried out analogously to example 5, with the only difference being that 10 ml of sorbent was used for purification.

Example 7. Depyrogenation of an aqueous protein solution

Preparation of the sorbent and purification of the aqueous protein solution was carried out analogously to example 5, with the only difference that the incubation was carried out at 20 ± 2 ° C.

Example 8. Depyrogenation of an aqueous protein solution

The sorbent was prepared analogously to Example 5. For purification, a solution of cytochrome C (1 mg / ml) in Tris-borate buffer solution (100 ml, 0.1 M, pH 8.3) contaminated with bacterial endotoxin (30 U / ml, E coli O113: H10). The purification was carried out by analogy with example 5 at 37 ± 2 ° C. Protein content was evaluated spectrophotometrically.

Example 9. Depyrogenation of an aqueous protein solution

The preparation of the sorbent was carried out analogously to example 5. A solution of hemoglobin (1 mg / ml) in a phosphate buffer solution (100 ml, 0.1 M, pH 7.0) contaminated with bacterial endotoxin (30 EU / ml, E. coli O113 was used for purification) : H10). The purification was carried out by analogy with example 5 at 37 ± 2 ° C. Protein content was evaluated spectrophotometrically.

Example 10. Depyrogenation of an aqueous solution of an inorganic salt

The sorbent was prepared analogously to Example 5. For purification, physiological NaCl solution (0.9%) was used, contaminated with bacterial endotoxin (KSE, 30 U / ml, E. coli). The purification was carried out by analogy with example 5 at 37 ± 2 ° C.

Example 11. Depyrogenation of an aqueous solution of an inorganic salt

Preparation of the sorbent and purification of an aqueous solution of inorganic salt was carried out analogously to example 10, with the only difference being that 10 ml of sorbent was used for purification.

Example 12. Depyrogenation of an aqueous solution of an inorganic salt

Preparation of the sorbent and purification of the aqueous protein solution was carried out analogously to example 10, with the only difference being that the incubation was carried out at 20 ± 2 ° C.

Example 13. Depyrogenation of blood plasma

Sorbent preparation was carried out analogously to Example 5. For purification, bovine blood plasma contaminated with bacterial endotoxin (5 U / ml, E. coli O113: H10) was used. The purification was carried out by analogy with example 5 at 37 ± 2 ° C.

Example 14. Depyrogenation of blood plasma

Preparation of the sorbent and purification of blood plasma was carried out analogously to example 13, with the only difference being that 10 ml of sorbent was used for purification.

Example 15. Depyrogenation of blood plasma

The preparation of the sorbent and purification of blood plasma was carried out analogously to example 13, with the only difference that the incubation was carried out at 20 ± 2 ° C.

The invented sorption material forms both ionic and hydrophobic bonds with the molecules of bacterial endotoxins, which provides a synergistic effect upon their binding and capture, which ensures high adsorption activity of the material in a wide range of external conditions (solution concentration, pH, ionic strength).

In conclusion, it is again important to mention that the removal of bacterial endotoxins is important in solving two problems:

1) treatment of sepsis and a number of other diseases by filtering the patient’s blood through a sorbent (hemosorption);

2) purification of pharmaceutical substances and injection solutions.

In the new proposed solution, sorption material (sorbent) is used to solve the second problem - purification (filtering) of aqueous solutions of pharmaceuticals, including protein nature, which may contain bacterial endotoxins. Therefore, inventions where sorbents are used to treat sepsis have not been considered as direct analogues.

The method that is used to purify pharmaceutical substances is one or another variation of affinity chromatography. In this case, a somewhat simplified cleaning procedure is proposed for patenting, associated with immersion of the sorbent in a vessel with the solution to be purified, in contrast to classical chromatography, when the solution to be purified is passed through the sorbent.

It should be noted the novelty of ligands that are immobilized on a carrier. In general, amines have been used to remove endotoxins for a long time, but the proposed solution uses specific synthetic amines that have all the signs of novelty to solve this problem.

All sorption processes take place outside the body and are classic examples of adsorbate-adsorbent interaction: the essence of the sorbent is that it firmly binds (removes from the solution) unwanted molecules (bacterial endotoxins), while it is not able to bind useful molecules (drugs and biological components liquids).

The proposed technical solution was obtained as part of the work under Agreement No. 14.577.21.0165 between the Ministry of Education and Science of the Russian Federation (State customer) and MSTU. N.E. Bauman.

Claims (9)

1. Sorption material, which is a porous polymer carrier, the functional groups on the surface of which are covalently linked to the ligand in an amount of from 1% to 25% by weight, capable of forming strong complexes with bacterial endotoxins, while the porous polymer carrier is made in the form of granules size from 50 to 900 microns from a polymer or copolymer obtained from monomers selected from the series: acrylic acid, methacrylic acid, acrylamide, methacrylamide, methyl acrylate, methyl methacrylate, glycidyl methacrylate, in nyl acetate, allyl amine, sodium 2-methylprop-2-en-1-sulfonate, allyl glycidyl ether, divinylbenzene, ethylene glycol dimethacrylate, triethylene glycol dimethacrylate, N, N-bis (methacrylamide), as a ligand for the binding of bacterial endotoxins, it contains organic endotoxins, a compound containing primary and secondary amino groups and hydrophobic substituents represented by the following formula:

where R is an aliphatic unbranched radical C _n H _{2n + 1} , n ranges from 1 to 18; benzyl; 4-chlorobenzyl; 4-phenylbenzyl; 4-methylbenzyl; the ratio of x + y lies in the range from 1 to 4; the ratio of y to x ranges from 0 to 1. 2. The method of obtaining the sorption material described in paragraph 1, which consists in polymerizing components or mixtures of components selected from the series: acrylic acid, methacrylic acid, acrylamide, methacrylamide, methyl acrylate, methyl methacrylate, glycidyl methacrylate, vinyl acetate, allylamine, sodium 2-methylprop-2-en-1-sulfonate, allyl glycidyl ether, divinylbenzene, ethylene glycol dimethacrylate, triethylene glycol dimethacrylate, N, N-bis (methacrylamide), the polymerization being carried out in the presence of compounds selected from cyclohexanol, toluene, polyvinyl alcohol, polyvinylpyrrolidone or mixtures thereof, at a temperature of 70-80 ° C; ligand synthesis is carried out separately using one of the following components: ethylene diamine, diethylene triamine, triethylenetetramine or tetraethylene pentamine, which are mixed with one of the following components: octadecyl bromide, dodecyl bromide, octyl bromide, butyl bromide, methyl iodide, benzyl chloride, 4-chlorobenzyl 4-methylbenzyl chloride, 4-phenylbenzyl chloride, the reaction is carried out in ethanol as a solvent during boiling, then the synthesized ligand is immobilized on a polymer carrier at room temperature or boiling in ethane ole. 3. The use of the sorption material described in paragraph 1, for the method of purification of an aqueous protein solution, or an aqueous saline solution, or a blood plasma solution, according to which 50-100 ml of contaminated solution is placed in a vessel containing 10-40 ml of sorption material, a temperature of 20-37 ° C for a period of from one minute to three hours, after which the solution is separated by filtration.