NZ711538A - Benzylisoquinoline alkaloids (bia) producing microbes, and methods of making and using the same - Google Patents

Benzylisoquinoline alkaloids (bia) producing microbes, and methods of making and using the same

Info

Publication number
NZ711538A
NZ711538A NZ711538A NZ71153814A NZ711538A NZ 711538 A NZ711538 A NZ 711538A NZ 711538 A NZ711538 A NZ 711538A NZ 71153814 A NZ71153814 A NZ 71153814A NZ 711538 A NZ711538 A NZ 711538A
Authority
NZ
New Zealand
Prior art keywords
host cells
methods
bias
bia
making
Prior art date
Application number
NZ711538A
Inventor
Christina D Smolke
Catherine Thodey
Isis Trenchard
Stephanie Galanie
Original Assignee
Univ Leland Stanford Junior
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=50928241&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=NZ711538(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ Leland Stanford Junior filed Critical Univ Leland Stanford Junior
Publication of NZ711538A publication Critical patent/NZ711538A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/182—Heterocyclic compounds containing nitrogen atoms as the only ring heteroatoms in the condensed system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y121/00—Oxidoreductases acting on X-H and Y-H to form an X-Y bond (1.21)
    • C12Y121/03—Oxidoreductases acting on X-H and Y-H to form an X-Y bond (1.21) with oxygen as acceptor (1.21.3)
    • C12Y121/03003—Reticuline oxidase (1.21.3.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/01—Methyltransferases (2.1.1)
    • C12Y201/01116—3'-Hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase (2.1.1.116)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/01—Methyltransferases (2.1.1)
    • C12Y201/01128—(RS)-Norcoclaurine 6-O-methyltransferase (2.1.1.128)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/01—Methyltransferases (2.1.1)
    • C12Y201/0114—(S)-Coclaurine-N-methyltransferase (2.1.1.140)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Mycology (AREA)

Abstract

Aspects of the invention include host cells that are engineered to produce benzylisoquinoline alkaloids (BIAs). The host cells include heterologous coding sequences for a variety of enzymes involved in synthetic pathways from starting compounds to BIAs of the host cell. Also provided are methods of producing the BIAs of interest by culturing the host cells under culture conditions that promote expression of enzymes encoded by the heterologous coding sequences of the host cells. Aspects of the invention further include compositions, e.g., host cells, starting compounds and kits, etc., that find use in methods of the invention.
NZ711538A 2013-03-15 2014-03-14 Benzylisoquinoline alkaloids (bia) producing microbes, and methods of making and using the same NZ711538A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361788560P 2013-03-15 2013-03-15
PCT/US2014/027833 WO2014143744A2 (en) 2013-03-15 2014-03-14 Benzylisoquinoline alkaloids (bia) producing microbes, and methods of making and using the same

Publications (1)

Publication Number Publication Date
NZ711538A true NZ711538A (en) 2017-03-31

Family

ID=50928241

Family Applications (1)

Application Number Title Priority Date Filing Date
NZ711538A NZ711538A (en) 2013-03-15 2014-03-14 Benzylisoquinoline alkaloids (bia) producing microbes, and methods of making and using the same

Country Status (14)

Country Link
US (6) US9534241B2 (en)
EP (3) EP3865580A1 (en)
JP (4) JP2016512046A (en)
CN (2) CN113980826A (en)
AU (2) AU2014228241B2 (en)
BR (1) BR112015023036A2 (en)
CA (1) CA2905112A1 (en)
DK (1) DK3553177T3 (en)
GB (1) GB2527254B (en)
HK (1) HK1220222A1 (en)
IL (1) IL240850B (en)
NZ (1) NZ711538A (en)
SI (1) SI3553177T1 (en)
WO (1) WO2014143744A2 (en)

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CN113980826A (en) 2022-01-28
US20180334695A1 (en) 2018-11-22
CN105247038A (en) 2016-01-13
US11214819B2 (en) 2022-01-04
GB2527254A (en) 2015-12-16
GB201518138D0 (en) 2015-11-25
AU2014228241B2 (en) 2017-09-21
EP3553177A1 (en) 2019-10-16
CA2905112A1 (en) 2014-09-18
GB2527254B (en) 2020-07-15
US10988787B2 (en) 2021-04-27
JP2024009200A (en) 2024-01-19
WO2014143744A2 (en) 2014-09-18
CN105247038B (en) 2021-08-27
JP2019162124A (en) 2019-09-26
BR112015023036A2 (en) 2017-11-14
US20210189445A1 (en) 2021-06-24
US20190071701A1 (en) 2019-03-07
IL240850B (en) 2020-05-31
JP2016512046A (en) 2016-04-25
WO2014143744A3 (en) 2015-11-05
JP2022023240A (en) 2022-02-07
EP2970999A2 (en) 2016-01-20
EP3865580A1 (en) 2021-08-18
DK3553177T3 (en) 2021-02-15
EP3553177B1 (en) 2020-12-09
IL240850A0 (en) 2015-10-29
AU2014228241A1 (en) 2015-09-17
US20170145454A1 (en) 2017-05-25
US20140273109A1 (en) 2014-09-18
US20220154232A1 (en) 2022-05-19
US9534241B2 (en) 2017-01-03
US10017799B2 (en) 2018-07-10
SI3553177T1 (en) 2021-03-31
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