NZ304538A - Process for producing a modified recombinant protein in a host cell comprising preparing a single vector encoding both the exogenous protein and an enzyme capable of modifying the exogenous protein - Google Patents

Process for producing a modified recombinant protein in a host cell comprising preparing a single vector encoding both the exogenous protein and an enzyme capable of modifying the exogenous protein

Info

Publication number
NZ304538A
NZ304538A NZ304538A NZ30453896A NZ304538A NZ 304538 A NZ304538 A NZ 304538A NZ 304538 A NZ304538 A NZ 304538A NZ 30453896 A NZ30453896 A NZ 30453896A NZ 304538 A NZ304538 A NZ 304538A
Authority
NZ
New Zealand
Prior art keywords
protein
exogenous protein
modifying
preparing
producing
Prior art date
Application number
NZ304538A
Inventor
Pradip Mukerji
Jennifer Marie Thurmond
Stig Lennart Hansson
Original Assignee
Abbott Lab
Symbicom Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Lab, Symbicom Ab filed Critical Abbott Lab
Publication of NZ304538A publication Critical patent/NZ304538A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4732—Casein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
NZ304538A 1995-02-27 1996-02-27 Process for producing a modified recombinant protein in a host cell comprising preparing a single vector encoding both the exogenous protein and an enzyme capable of modifying the exogenous protein NZ304538A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39499995A 1995-02-27 1995-02-27
PCT/US1996/002623 WO1996027017A1 (en) 1995-02-27 1996-02-27 A method for expressing modified recombinant proteins in a bacterial system

Publications (1)

Publication Number Publication Date
NZ304538A true NZ304538A (en) 1999-08-30

Family

ID=23561282

Family Applications (1)

Application Number Title Priority Date Filing Date
NZ304538A NZ304538A (en) 1995-02-27 1996-02-27 Process for producing a modified recombinant protein in a host cell comprising preparing a single vector encoding both the exogenous protein and an enzyme capable of modifying the exogenous protein

Country Status (5)

Country Link
EP (1) EP0812361A1 (en)
JP (1) JPH11500918A (en)
CA (1) CA2213857A1 (en)
NZ (1) NZ304538A (en)
WO (1) WO1996027017A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5807702A (en) * 1995-02-27 1998-09-15 Abbott Laboratories Method for expressing phosphorylated recombinant human β-casein in a bacterial system
US6071718A (en) * 1996-11-27 2000-06-06 Abbott Laboratories Methods of producing a recombinant protein
US6287866B1 (en) 1996-11-27 2001-09-11 Abbott Laboratories β-casein expressing constructs
US6121421A (en) * 1998-08-21 2000-09-19 Abbott Laboratories Methods for isolating recombinant β-casein

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0178863A1 (en) * 1984-10-15 1986-04-23 Schering Corporation Novel expression systems utilizing bacteriophage T7 promoters and gene sequences
JPH06245775A (en) * 1990-02-26 1994-09-06 Washington Univ Method for myristoylation of protein n
EP0574402B2 (en) * 1990-11-26 2012-08-01 Genetics Institute, LLC Expression of pace in host cells and methods of use thereof
EP0548012B1 (en) * 1991-12-16 1997-09-03 Novartis AG Endoplastic reticulum-located recombinant dibasic endoprotease and uses thereof
US5512434A (en) * 1992-12-14 1996-04-30 The United States Of America As Represented By The Department Of Health And Human Services Expression cloning of a human phosphatase

Also Published As

Publication number Publication date
JPH11500918A (en) 1999-01-26
WO1996027017A1 (en) 1996-09-06
CA2213857A1 (en) 1996-09-06
EP0812361A1 (en) 1997-12-17

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