NL2029910B9 - Extraction method of seabuckthorn leaves extract - Google Patents

Extraction method of seabuckthorn leaves extract Download PDF

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Publication number
NL2029910B9
NL2029910B9 NL2029910A NL2029910A NL2029910B9 NL 2029910 B9 NL2029910 B9 NL 2029910B9 NL 2029910 A NL2029910 A NL 2029910A NL 2029910 A NL2029910 A NL 2029910A NL 2029910 B9 NL2029910 B9 NL 2029910B9
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Prior art keywords
extraction
sea buckthorn
leaf extract
buckthorn leaf
leaves
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NL2029910A
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Dutch (nl)
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NL2029910B1 (en
Inventor
Feng Lidan
Chai Shouhuan
Li Jie
Mi Lan
Jiang Yumei
Sheng Wenjun
Bi Yang
Li Jixin
Wei Juan
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Univ Gansu Agricultural
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B01D11/0203—Solvent extraction of solids with a supercritical fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B01D11/0288—Applications, solvents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B01D11/0292—Treatment of the solvent
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B9/00—Essential oils; Perfumes
    • C11B9/02—Recovery or refining of essential oils from raw materials
    • C11B9/025—Recovery by solvent extraction

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Extraction Or Liquid Replacement (AREA)

Abstract

The present invention discloses an extraction method of seabuckthorn leaves extract, including: step one, drying seabuckthom leaves, crushing it, and sieving it to obtain seabuckthom leaves powder, step two, performing subcritical extraction of the seabuckthom leaves powder, and extracting it for 3 times to obtain an extracting solution, step three, separating the extracting solution to obtain seabuckthom leaves extract. The extraction method of the present invention has simple process, rapid extraction speed, mild extraction conditions, and high extraction yield, and solves the problems of long extraction time, high extraction temperature, low quality of extract and high cost in prior extraction technologies.

Description

EXTRACTION METHOD OF SEABUCKTHORN LEAVES EXTRACT
TECHNICAL FIELD
The present invention relates to the field of the extraction of extract. More particularly, the present invention relates to an extraction method of seabuckthorn leaves extract.
BACKGROUND
Seabuckthorn leaves are extremely enriched with nutrients and physiologically active substances, mainly including proteins, polysaccharides, organic acids, alkaloids, flavonoids, amino acids, carotenoids, chlorophyll, trace elements, etc., which can regulate blood lipids, inhibit bacteria, scavenge free radicals, remove sputum, relieve cough and relieve asthma, etc., and have very significant medicinal value. Extract refers to perfumery products obtained by extracting perfume plant tissues (such as flowers, leaves, branches, stems, barks, roots, fruits, etc.) that do not contain exudates with organic solvents, which is enriched with nutrients and active substances, and 1s an ideal base material for the separation of active ingredients.
Currently, the extraction methods of extract include traditional extraction and supercritical extraction. However, the traditional extraction has complicated process, long extraction time, high extraction temperature, low extraction efficiency, and low quality of extract, and the supercritical extraction has high production cost and cannot meet the requirements of markets.
SUMMARY
An object of the present invention is to solve at least above problems, and to provide, at least, the advantages that will be described later.
An another object of the present invention is to provide an extraction method of seabuckthorn leaves extract, which adopts subcritical extraction with 1,1,1,2-tetrafluoroethane, solves the problems of long extraction time, high extraction temperature, low quality of extract and high cost in prior extraction technologies, and provides high-quality samples for the subsequent separation and purification of active 1 components.
In order to achieve the above object, the present invention provides an extraction method of seabuckthorn leaves extract, including the following steps of: step one, drying seabuckthorn leaves, crushing it, and sieving it to obtain seabuckthorn leaves powder; step two, performing subcritical extraction of the seabuckthorn leaves powder, and extracting it for 3 times to obtain an extracting solution; step three, separating the extracting solution to obtain seabuckthorn leaves extract.
Preferably, in the step two, wherein an extraction pressure of the subcritical extraction is 0.7 MPa, and an extraction temperature is 50-55°C.
Preferably, the step two specifically includes: placing the seabuckthorn leaves powder packed with an extraction bag in an extraction kettle, closing a feeding valve, opening an air exhaust valve, closing the air exhaust valve after exhausting air of the extraction kettle, opening a liquid inlet valve, adding an extraction solvent into a solvent tank, heating it to reach a subcritical state, introducing it into the extraction kettle, adjusting an extraction pressure of the extraction kettle to a preset pressure, and performing extraction.
Preferably, the extraction solvent is 1,1,1,2-tetrafluoroethane, which is delivered by a high-pressure plunger pump until the seabuckthorn leaves powder is immersed.
Preferably, the extraction kettle is subjected to exhausting air until a pressure in the extraction kettle is reduced to -0.5 MPa.
Preferably, in the step two, the subcritical extraction is completed for 3 times within 60-90 min.
Preferably, in the step one, drying seabuckthorn leaves includes drying the seabuckthorn leaves at room temperature until the moisture content is below 5%.
Preferably, in the step one, crushed seabuckthorn leaves are subjected to sieving with a 20-40 mesh sieve to obtain the seabuckthorn leaves powder.
Preferably, the step three specifically includes: introducing the extracting solution into a separation kettle, increasing temperature under reduced pressure for vaporizing and separating the solvent, and mixing separated products of three subcritical extractions to obtain the seabuckthom leaves extract. 2 wherein the vaporized solvent can be pressurized, condensed, liquefied and re-entered into the solvent tank for recycling.
The present invention includes at least the following advantages:
The extraction method of seabuckthorn leaves extract of the present invention has simple process, rapid extraction speed, mild extraction conditions, and high extraction yield more than 2.5%. The extraction solvent can be recycled, and the extraction solvent has high recycling rate in a production process, which can effectively protect the ecological environment and reduce production costs.
Other advantages, objects and features of the present invention will be partially reflected by the following description, and will be partially understood by those skilled in the art through researching and practicing the present invention.
BRIEF DESCRIPTION OF THE DRAWINGS
Fig.l is a flow diagram according to an extraction method of seabuckthorn leaves extract of the present invention.
DETAILED DESCRIPTION
The present invention will now be described in further detail with reference to the accompanying drawings in order to enable person skilled in the art to practice with reference tothe summary.
It should be noted that terms such as "having", "including" and "comprising" as used herein do not exclude presence or addition of one or more other elements or combinations thereof.
Embodiment 1
An extraction method of seabuckthorn leaves extract, including the following steps of: drying seabuckthorn leaves at room temperature until a moisture content is below 5%, crushing it with a crusher, sieving it with a 20 mesh sieve to obtain seabuckthorn leaves powder, weighing 500 g, packing it into an extraction bag, placing it in an extraction kettle with a capacity of 3 L, closing a feeding valve, opening an air exhaust valve, exhausting air in the extraction kettle through a vacuum pump until a pressure in the extraction kettle is 3 reduced to -0.5 MPa, closing the air exhaust valve, opening a liquid inlet valve, delivering
R134a (1,1,1,2-tetrafluoroethane) into a solvent tank through a high-pressure plunger pump, heating it with a plate heat exchanger to 50°C until R134a reaches a subcritical state, then injecting it into the extraction kettle through the high-pressure plunger pump until the seabuckthorn leaves powder is immersed by observing a liquid level of the solvent through a sight glass, adjusting an extraction pressure of the extraction kettle to 0.7 MPa, and performing extraction for 3 times within 60 min to obtain an extracting solution. After the extraction, the extracting solution is transferred to a separation kettle, and is subjected to increasing temperature under reduced pressure for vaporizing and separating the solvent, wherein the solvent after being pressurized, condensed, liquefied can be re-entered into the solvent tank for recycling. Separated products of the subcritical extraction for 3 times are mixed to obtain the seabuckthom leaves extract with a yield of 2.5%.
Embodiment 2
An extraction method of seabuckthorn leaves extract are the same as that in the
Embodiment 1, and the difference is: performing extraction for 3 times within 90 min, and obtained seabuckthorn leaves extract has a yield of 3.1%. Other processes and parameters are exactly the same as that in the Embodiment 1.
Embodiment 3
An extraction method of seabuckthorn leaves extract are the same as that in the
Embodiment 2, and the difference is: sieving it with a 40 mesh, and obtained seabuckthorn leaves extract has a yield of 3.5%. Other processes and parameters are exactly the same as that in the Embodiment 2.
Embodiment 4
An extraction method of seabuckthorn leaves extract are the same as that in the
Embodiment 3, and the difference is: heating it with a plate heat exchanger to 55°C, and obtained seabuckthorn leaves extract has a yield of 3.9%. Other processes and parameters are exactly the same as that in the Embodiment 3.
Comparative example 1
An extraction method of seabuckthorn leaves extract are the same as that in the
Embodiment 1, and the difference is: sieving it with a 10 mesh, heating it with a plate heat 4 exchanger to 40°C, and performing extraction for 3 times within 30 min. Obtained seabuckthom leaves extract has a yield of 1.8%. Other processes and parameters are exactly the same as that in the Embodiment 1.
Comparative example 2
An extraction method of seabuckthorn leaves extract are the same as that in the
Embodiment 1, and the difference is: sieving it with a 100 mesh, heating it with a plate heat exchanger to 60°C, and performing extraction for 3 times within 100 min. Obtained seabuckthorn leaves extract has a yield of 2.2%. Other processes and parameters are exactly the same as that in the Embodiment 1.
Although the embodiments of the present invention have been disclosed above, they are not limited to the applications previously mentioned in the specification and embodiments, and can be applied in various fields suitable for the present invention. For ordinary skilled person in the field, other various changed model, formula and parameter may be easily achieved without creative work according to instruction of the present invention, changed, modified and replaced embodiments without departing the general concept defined by the claims and their equivalent are still included in the present invention. The present invention is not limited to particular details and illustrations shown and described herein. 5

Claims (9)

CONCLUSIESCONCLUSIONS 1. Extractiewerkwijze van duindoornbladextract, omvattende de volgende stappen: - stap één: het drogen van duindoornbladeren, het pletten ervan en het zeven ervan om een duindoornbladpoeder te verkrijgen; - stap twee: het uitvoeren van subkritische extractie van het duindoornbladpoeder en het 3 keer extraheren om een extractieoplossing te verkrijgen;; - stap drie: het scheiden van de extractieoplossing om een duindoornbladextract te verkrijgen.1. Extraction method of sea buckthorn leaf extract, comprising the following steps: - step one: drying sea buckthorn leaves, crushing them and sieving them to obtain a sea buckthorn leaf powder; - step two: performing subcritical extraction of the sea buckthorn leaf powder and extracting it 3 times to obtain an extraction solution;; - step three: separating the extraction solution to obtain a sea buckthorn leaf extract. 2. Extractiewerkwijze van duindoornbladextract volgens conclusie 1, met het kenmerk, dat in stap twee een extractiedruk van de subkritische extractie 0,7 MPa is, en een extractietemperatuur 50 tot 55 °C is.Sea buckthorn leaf extract extraction method according to claim 1, characterized in that in step two an extraction pressure of the subcritical extraction is 0.7 MPa, and an extraction temperature is 50 to 55 °C. 3. Extractiewerkwijze van duindoornbladextract volgens conclusie 1, met het kenmerk, dat stap twee specifiek omvat: het plaatsen van het duindoornbladpoeder verpakt met een extractiezak in een extractieketel, het sluiten van een voedingsklep, het openen van een luchtafvoerklep, het sluiten van de luchtafvoerklep na het afvoeren van lucht uit de extractieketel, het openen van een vloeistofinlaatklep, het toevoegen van een extractieoplosmiddel in een oplosmiddeltank, het verwarmen ervan om een subkritische toestand te bereiken, het inbrengen ervan in de extractieketel, het instellen van een extractiedruk van de extractieketel op een vooraf ingestelde druk, en het uitvoeren van extractie.3. Sea buckthorn leaf extract extraction method according to claim 1, characterized in that step two specifically includes: placing the sea buckthorn leaf powder packed with an extraction bag into an extraction vessel, closing a feeding valve, opening an air exhaust valve, closing the air exhaust valve after removing air from the extraction boiler, opening a liquid inlet valve, adding an extraction solvent into a solvent tank, heating it to reach a subcritical condition, introducing it into the extraction boiler, setting an extraction pressure of the extraction boiler to a preset pressure, and performing extraction. 4. Extractewerkwijze van duindoornbladextract volgens conclusie 3, met het kenmerk, dat het extractieoplosmiddel 1,1,1,2-tetrafluorethaan is, dat wordt geleverd door een hogedrukplunjerpomp totdat het duindoornbladpoeder is ondergedompeld.Sea buckthorn leaf extract extraction method according to claim 3, characterized in that the extraction solvent is 1,1,1,2-tetrafluoroethane, which is supplied by a high-pressure plunger pump until the sea buckthorn leaf powder is immersed. 5. Extractiewerkwijze van duindoornbladextract volgens conclusie 3, met het kenmerk, dat de extractieketel wordt onderworpen aan luchtafvoer totdat een druk in de extractieketel is verminderd tot -0,5 MPa.Extraction method of sea buckthorn leaf extract according to claim 3, characterized in that the extraction kettle is subjected to air exhaust until a pressure in the extraction kettle is reduced to -0.5 MPa. 6. Extractiewerkwijze van duindoornbladextract volgens conclusie 1, met het kenmerk, dat de subkritische extractie 3 keer binnen 60 tot 90 minuten wordt voltooid. 6Sea buckthorn leaf extract extraction method according to claim 1, characterized in that the subcritical extraction is completed 3 times within 60 to 90 minutes. 6 7. Extractiewerkwijze van duindoornbladextract volgens conclusie 1, met het kenmerk, dat in stap één het drogen van duindoornbladeren het drogen van de duindoornbladeren bij kamertemperatuur omvat totdat een vochtgehalte van de duindoornbladeren lager is dan 5 %.The extraction method of sea buckthorn leaf extract according to claim 1, characterized in that in step one the drying of sea buckthorn leaves comprises drying the sea buckthorn leaves at room temperature until a moisture content of the sea buckthorn leaves is lower than 5%. 8. Extractiewerkwijze van duindoornbladextract volgens conclusie 1, met het kenmerk, dat in stap één de geplette duindoornbladeren worden gezeefd met een zeef van 20 tot 40 mesh om het duindoornbladpoeder te verkrijgen.The extraction method of sea buckthorn leaf extract according to claim 1, characterized in that in step one the crushed sea buckthorn leaves are sieved with a 20 to 40 mesh sieve to obtain the sea buckthorn leaf powder. 9. Extractiewerkwijze van duindoornbladextract volgens conclusie 1, met het kenmerk, dat stap drie specifiek omvat: het inbrengen van de extractieoplossing in een scheidingsketel, het verhogen van de temperatuur onder verlaagde druk voor het verdampen en scheiden van het oplosmiddel, en het mengen van gescheiden producten van drie subkritische extracties om het duindoornbladextract te verkrijgen, waarbij het verdampte oplosmiddel onder druk kan worden gebracht, gecondenseerd, vloeibaar gemaakt en opnieuw in de oplosmiddeltank kan worden gebracht voor recycling. 7The extraction method of sea buckthorn leaf extract according to claim 1, characterized in that step three specifically comprises: introducing the extraction solution into a separation vessel, raising the temperature under reduced pressure to evaporate and separate the solvent, and mixing separated products of three subcritical extractions to obtain the sea buckthorn leaf extract, where the evaporated solvent can be pressurized, condensed, liquefied and returned to the solvent tank for recycling. 7
NL2029910A 2021-11-25 2021-11-25 Extraction method of seabuckthorn leaves extract NL2029910B9 (en)

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CN101225080A (en) * 2008-01-25 2008-07-23 东北农业大学 Method for extracting flavone from sea-buckthorn leaves and feed stuff containing the same
CN106753772A (en) * 2017-02-23 2017-05-31 甘肃农业大学 A kind of method and compound product of low-temperature extraction Seabuckthorm Seed Oil
CN108272837A (en) * 2018-03-16 2018-07-13 忻州师范学院 The extracting method of flavone compound in a kind of Leaves of Hippophae L
CN108771068A (en) * 2018-05-24 2018-11-09 小金县四姑娘山天然沙棘食品有限公司 A kind of sugar-free seabuckthorn leaf beverage and preparation method thereof
CN109852481B (en) * 2019-04-19 2022-02-18 上海为止生物科技有限公司 Herbal low-temperature extraction process and toothpaste prepared by same and used for treating periodontitis
CN110477197A (en) * 2019-09-02 2019-11-22 邳州市东方养殖有限公司 A kind of preparation method and purposes of laying hen compound formulation of astragalus root composition
CN113058401B (en) * 2021-03-17 2022-11-29 毕晖 Composite plant active negative oxygen ion liquid and preparation method thereof

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