KR890701628A - Preparation of Protein (Sex) Sweeteners - Google Patents

Preparation of Protein (Sex) Sweeteners

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Publication number
KR890701628A
KR890701628A KR1019890700292A KR890700292A KR890701628A KR 890701628 A KR890701628 A KR 890701628A KR 1019890700292 A KR1019890700292 A KR 1019890700292A KR 890700292 A KR890700292 A KR 890700292A KR 890701628 A KR890701628 A KR 890701628A
Authority
KR
South Korea
Prior art keywords
amino acids
polypeptide
arrangement
substitution
amino acid
Prior art date
Application number
KR1019890700292A
Other languages
Korean (ko)
Inventor
김성호
조중명
Original Assignee
원본미기재
럭키 바이오 테크 코포레이션
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 원본미기재, 럭키 바이오 테크 코포레이션 filed Critical 원본미기재
Publication of KR890701628A publication Critical patent/KR890701628A/en

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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/43Sweetening agents, e.g. thaumatin, monellin

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

내용 없음No content

Description

단백질(성) 감미제의 제조Preparation of Protein (Sex) Sweeteners

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (14)

펩티드결합을 통해 함께 공유결합된 모넬린 서브유니트의 아미노산배열을 적어도 80% 가지고 있는 아미노산배열로 이루어져 있는 폴리펩티드를 감미제로써 포함하는 단백질성 감미제의 성분.A component of a proteinaceous sweetener comprising a polypeptide consisting of an amino acid sequence having at least 80% amino acid sequence of a moneline subunit covalently bonded together via peptide bonds. 제1항에 있어서, 감미제가 N-말단으로써 서브유니트 Ⅱ의 배열 및 C-말단으로써 서브유니트 Ⅰ의 배열을 갖음을 특징으로 하는 성분.2. A component according to claim 1, wherein the sweetener has an arrangement of subunits II at the N-terminus and a subunit I arrangement at the C-terminus. 제2항에 있어서, 오넬린의 다양한 배열중에 ile(46) 내지 gly(6) 사이에 낀 배열이 많아야 전부 10개의 아미노산으로 이루어지는 치환, 삭제 또는 삽입에 의해 변경됨을 특징으로 하는 성분.3. A component according to claim 2, characterized in that, among the various arrangements of onelin, the arrangement between ile (46) and gly (6) is at most altered by substitution, deletion or insertion consisting of all ten amino acids. 제3항에 있어서, 변경이 1개 내지 5개 아미노산의 삭제로 이루어짐을 특징으로 하는 성분.4. A component according to claim 3, wherein the alteration consists of deletion of 1 to 5 amino acids. 제3항에 있어서, 변경이 적어도 1개 아미노산의 적어도 한 치환으로 이루어짐을 특징으로 하는 성분.4. A component according to claim 3, wherein the alteration consists of at least one substitution of at least one amino acid. 제5항에 있어서, 치환에 속하는 아미노산의 적어도 50%가 극성 아미노산임을 특징으로 하는 성분.The component of claim 5, wherein at least 50% of the amino acids belonging to the substitution are polar amino acids. 폴리펩티드 결합을 통해 함께 공유결합된 모넬린 서브유니트의 아미노산배열을 적어도 80% 가지고 있는 아미노산 배열로 구성되는 폴리펩티드.A polypeptide consisting of an amino acid sequence having at least 80% amino acid sequence of a monelin subunit covalently bonded together via a polypeptide bond. 제7항에 있어서, N-말단으로써 서브유니트 Ⅱ의 배열 및 C-말단으로써 서브유니트 Ⅰ의 배열을 갖음을 특징으로 하는 폴리펩티드.8. A polypeptide according to claim 7, having an arrangement of subunits II at the N-terminus and an arrangement of subunits I at the C-terminus. 제8항에 있어서, 모넬린의 다양한 배열중에 Tyr(41) 내지 leu(3)의 사이에 낀 배열이 많아야 전부 10개의 아미노산으로 이루어지는 치환, 삭제 또는 삽입에 의해 변경됨을 특징으로 하는 폴리펩티드.9. A polypeptide according to claim 8, wherein the arrangement between Tyr (41) and leu (3) at most among the various arrangements of monelin is altered by substitution, deletion or insertion consisting of all ten amino acids. 제9항에 있어서, 변경이 1개 내지 5개 아미노산의 삭제로 이루어짐을 특징으로 하는 폴리펩티드.The polypeptide of claim 9, wherein the alteration consists of deletion of 1 to 5 amino acids. 제9항에 있어서, 변경이 적어도 2개의 아미노산의 적어도 한 치환으로 이루어짐을 특징으로 하는 폴리펩티드.The polypeptide of claim 9, wherein the alteration consists of at least one substitution of at least two amino acids. 제11항에 있어서, 치환에 속하는 아미노산의 적어도 50%가 극성 아미노산임을 특징으로 하는 폴리펩티드.The polypeptide of claim 11, wherein at least 50% of the amino acids belonging to the substitution are polar amino acids. 제9항에 있어서, 사이에 낀 배열이 다음의 식으로 주어짐을 특징으로 하는 폴리펩티드.10. A polypeptide according to claim 9 wherein the array in between is given by the following formula. 상기식에서In the above formula 상기에서 x는 0 또는 1이고, 적어도 하나의 x는 1이다.Wherein x is 0 or 1 and at least one x is 1. 제13항에 있어서, 사이에 낀 배열이 Y-E-N-E-R-E-I-K이고; Y-E-N-R-E-D-I-K이고; Y-K-T-R-E-D-I-K이고; Y-E-R-E-I-K이고; Y-E-N-I-K이고; Y-E-I-K이고; Y-Y-A-S-D-K-L-K이고; Y-A-S-D-K-L이고; Y-A-S-D-K이고; Y-S-D-K이고; E-D-Y-K-T-R-G-R이고; 또는 E-D-Y-T-R임을 특징으로 하는 폴리펩티드.14. The arrangement of claim 13, wherein the arrangement in between is Y-E-N-E-R-E-I-K; Y-E-N-R-E-D-I-K; Y-K-T-R-E-D-I-K; Y-E-R-E-I-K; Y-E-N-I-K; Y-E-I-K; Y-Y-A-S-D-K-L-K; Y-A-S-D-K-L; Y-A-S-D-K; Y-S-D-K; E-D-Y-K-T-R-G-R; Or E-D-Y-T-R. ※ 참고사항: 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the original application.
KR1019890700292A 1987-06-19 1988-06-17 Preparation of Protein (Sex) Sweeteners KR890701628A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US6434387A 1987-06-19 1987-06-19
US064,343 1987-06-19
PCT/US1988/002114 WO1988010271A1 (en) 1987-06-19 1988-06-17 Preparation of novel protein sweeteners

Publications (1)

Publication Number Publication Date
KR890701628A true KR890701628A (en) 1989-12-21

Family

ID=22055288

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019890700292A KR890701628A (en) 1987-06-19 1988-06-17 Preparation of Protein (Sex) Sweeteners

Country Status (5)

Country Link
EP (1) EP0318580A4 (en)
JP (1) JPH02500641A (en)
KR (1) KR890701628A (en)
AU (1) AU618011B2 (en)
WO (1) WO1988010271A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5234834A (en) * 1987-06-19 1993-08-10 The Regents Of The University Of California Constructs for expression of monellin in plant cells
DE3850574T2 (en) * 1987-06-19 1994-10-27 Univ California NEW CLASS OF LOW-CALORINE PROTEIN INGREDIENTS.
US5739409A (en) * 1987-06-19 1998-04-14 The Regents Of The University Of California Endogenously sweetened transgenic plant products
AU619193B2 (en) * 1987-06-19 1992-01-23 Lucky Limited Preparation of novel protein sweeteners - monellin type

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1337086A (en) * 1971-05-25 1973-11-14 Tate & Lyle Ltd Sweet substance
US3998798A (en) * 1972-12-22 1976-12-21 The Trustees Of The University Of Pennsylvania Monellin, a sweet polypeptide derived from fruit of dioscoreophyllum cumminsii
CA1310923C (en) * 1985-11-13 1992-12-01 Joachim Ludwig Weickmann Dna encoding [asp --] and [lys -, asp --] thaumatin i
DE3850574T2 (en) * 1987-06-19 1994-10-27 Univ California NEW CLASS OF LOW-CALORINE PROTEIN INGREDIENTS.

Also Published As

Publication number Publication date
EP0318580A4 (en) 1990-09-26
EP0318580A1 (en) 1989-06-07
JPH02500641A (en) 1990-03-08
AU1994088A (en) 1989-01-19
WO1988010271A1 (en) 1988-12-29
AU618011B2 (en) 1991-12-12

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