KR20160105716A

KR20160105716A - Disposable container for bioreactor

Info

Publication number: KR20160105716A
Application number: KR1020160021847A
Authority: KR
Inventors: 김두현
Original assignee: 김두현
Priority date: 2015-02-28
Filing date: 2016-02-24
Publication date: 2016-09-07

Abstract

A disposable container of a cell culture system is disclosed. A disposable container of a cell culture system according to an embodiment of the present invention includes a cover having a cylindrical shape with at least one flexible structure having a bottom open and a cylindrical shape coupled with an open bottom side of the cover, And a base provided with at least one connection port projecting outwardly on the side thereof so that the structure of the supporting structure for self-standing can be minimized and a separate supporting structure for supporting the stirrer is not required, Time, effort and cost can be saved.

Description

Disposable Container for Bioreactors < RTI ID = 0.0 >

The present invention relates to a disposable container for use in a bioreactor, and more particularly, to a disposable container of a bioreactor capable of minimizing the installation of a support structure for supporting the bioreactor and supporting the stirrer itself.

Currently, various kinds of bioreactors including cell incubators are widely used in medicine, pharmacy, biotechnology and the like, such as the manufacture of reagents or vaccines, the development of new drugs, and the treatment and research using stem cells.

Bioreactor refers to a system that artificially reproduces biochemical reaction processes such as decomposition, synthesis, and chemical transformation of substances in the body of a living organism, and is also called a bioreactor.

Therefore, various conditions such as nutrients, temperature, humidity, pH, oxygen and carbon dioxide concentration suitable for the mixing of substances or cultivation of cells are actually maintained for a predetermined period according to various work contents performed by the bioreactor .

In case of using bioreactor to pollute the material that is being produced, it is common to dispose of all expensive materials that are in use. In particular, when a cell is cultured with a bioreactor, it is very rare that the cell itself has an immune system. Therefore, the inside of the bioreactor must be able to be kept in a shielded state so as not to be contaminated. Before the cell culture starts, the bioreactor is autoclaved or gamma-sterilized.

In the case of culturing animal cells, all components of the bioreactor that can be contacted with the cells must be made of an animal-derived component free (ADCF) clean material.

Since the components of the bioreactor having all of the above conditions are quite expensive, a large number of components other than consumables such as various connectors and connectors are repeatedly used.

However, once the operation such as cell culture is completed, the bioreactor can be reused after a number of procedures such as completely removing the residual material of the bioreactor and re-executing the above-mentioned sterilization step. Particularly, in the case of containers in which cells are directly accommodated and cultured, more stringent conditions are applied.

Such a process for reusing the bioreactor has a disadvantage in that a considerable time, cost, and effort are required.

In order to overcome these disadvantages, a disposable container which can be discarded once the cell culture is completed has been proposed.

However, since the proposed disposable container is in the same form as a 'plastic bag' made of a film, there is a disadvantage that a support structure supporting the entirety is required to maintain a constant shape of the container in order to cultivate the cells.

For example, Korean Patent Registration No. 10-1228026 discloses a support structure including a rod connected to the top of a container to support a bag-type container.

In addition, an agitator for circulating nutrients and oxygen is generally installed in the container. However, since the disposable container can not support the agitator, there is a disadvantage that a separate support structure for supporting the agitator must be provided.

For example, Korean Patent Laid-Open Publication No. 10-2012-0108054 discloses that a stirrer installed in a bag is fixed by a support housing.

In addition, the conventional disposable container is provided with a through hole formed in a container film for each portion where nutrients are supplied, waste is discharged, oxygen is supplied, and temperature and pH sensors are connected to each other. It takes a considerable amount of time to check the state, and there is also a possibility that structural defects are generated.

Korean Patent Registration No. 10-1228026 (entitled "Cell / tissue culture apparatus, system and method, registered date: January 24, 2013) Korean Patent Laid-Open Publication No. 10-2012-0108054 (Name of invention: Processed bag container with sensors, published on October 4, 2012)

An embodiment of the present invention seeks to provide a disposable container of a bioreactor capable of minimizing the installation of a support structure for supporting a disposable container of a bioreactor and supporting the stirrer by itself.

In the embodiment of the present invention, a connection part for connecting the disposable container of the bioreactor with the sensor for measuring the supply of nutrients, the discharge of waste products, the supply of oxygen, and the temperature and pH is formed in advance, Disposable containers of bioreactors that can save time and effort.

Also, an embodiment of the present invention is to provide a disposable container of a bioreactor in which the efficiency of a biological process can be improved.

Also, an embodiment of the present invention is to provide a disposable container of a bioreactor that can be folded.

According to an aspect of the present invention, there is provided a container comprising: a container-shaped cover made of at least one flexible structural layer and having a bottom open; a container shape coupled to the open lower side of the cover, And a lower end of the cover is coupled to an upper end of an edge of the base. The disposable container of the cell culture system may be provided in the container. At this time, a rounded portion may be formed on the inner lower edge of the base.

The cover may have a shape of a cylinder, an ellipse, a prism, a cone, or a pyramid, and the open top surface of the base may have a shape corresponding to the shape of the open bottom surface of the cover.

The cover and the base may be engaged with each other by fusion of the inner circumferential surface of the lower end of the cover to the outer circumferential surface of the upper end of the base of the cover or the outer circumferential surface of the lower end of the cover may be fused to the inner circumferential surface of the upper end of the base.

In this case, the upper end of the base may be formed with a number of engagement protrusions corresponding to the number of the structural layers along the outer circumference sequentially toward the outward direction or the inward direction of the base. At this time, an engaging protrusion or an engaging groove is formed in a portion of the base which is in contact with one surface or the other surface of the end portion of the structure layer, and one surface or the other surface of the structure layer is provided with the engaging projection or the engaging groove Corresponding engagement grooves or engaging projections can be formed.

Alternatively, a number of insertion grooves corresponding to the number of the structural layers may be sequentially formed in an upper end portion of the base at an edge of the base toward an outward direction or an inward direction of the base, and an end portion of the structure layer Can be inserted. At this time, a supporting rib supporting the end of the inserted structure layer may be protruded from the insertion groove.

The disposable container of the above-described cell culture system may further include the cover and the connection ring interposed in the base.

Here, the outer circumferential surface of the connecting ring is fused to the outer circumferential surface of the lower end portion of the cover, or the inner circumferential surface of the connecting ring is fused to the outer circumferential surface of the lower end portion of the cover, and a fastening protrusion or a fastening groove And a fastening groove corresponding to the fastening protrusion or a fastening protrusion corresponding to the fastening groove may be formed at the upper end of the edge of the base.

The disposable container of the cell culture system may further include a reinforcing ring having an inner circumferential surface welded to an outer circumferential surface of a lower end portion of the cover and a fastening protrusion formed at a lower side thereof, Wherein an engaging rib is protruded upward from an upper end of the base closer to the central portion of the base than the engaging groove, and the engaging rib is formed with a protruding portion, which protrudes upward from the reinforcing ring when the engaging projection is engaged with the engaging groove It can be further projected upward.

In the disposable container of the above-described cell culture system, one or more tensile grooves may be formed in the portion of the upper edge of the base which is fused to the lower end of the cover.

Here, the tensile groove may be a long groove extending in a direction parallel to the center axis of the base.

In the disposable container of the above-described cell culture system, a heat medium circulation channel is formed in the base, and at least one pair of heat medium circulation ports communicating with the heat medium circulation channel may be formed on an outer circumferential surface of the base.

In the disposable container of the above-described cell culture system, the base surrounds the side and bottom of the base and is coupled to the base. A heat medium circulation channel is formed in the inside of the disposable container, and at least one pair of heat medium circulation ports communicating with the heat medium circulation channel A formed base temperature adjusting means may be further included.

In the disposable container of the above-described cell culture system, the cover surrounds the outer circumferential surface of the cover and is supported by the base, and a heat medium circulation channel is formed therein, and at least one pair of heat medium circulation ports communicating with the heat medium circulation channel is formed on the outer circumference thereof The cover temperature adjusting means may be further included.

In the disposable container of the above-described cell culture system, at least one contact wire is formed on the cover, and when a vertical external force is applied to the cover, a portion of the cover where the contact wire is formed is folded, Can be shortened. At this time, the folded portion of the cover can be received inside the base.

In the disposable container of the cell culture system as described above, a fixing protrusion or a fixing groove may be formed in the middle of the inner bottom surface of the base. Here, the seating rib may protrude from the outer circumference of the fixing protrusion, or may have a seating recess.

Or a disposable container of a cell culture system, wherein a cap coupling hole is formed on at least a part of an upper surface of the cover, and a cap coupled to the cap coupling hole is further included, and an edge portion of the cap coupling hole Can be formed. Here, a shaft hole may be formed through the intermediate portion of the cap.

According to the embodiment of the present invention, since the structure of the support structure for self-standing can be minimized and a separate support structure for supporting the stirrer is not required, the time, effort and cost required to construct the bioreactor Can be saved.

In addition, according to the embodiment of the present invention, since the connection part for connecting the various connection pipes is formed in advance, the time and effort required for preparing the work using the bioreactor can be greatly reduced.

In addition, according to the embodiment of the present invention, the formation of dead zones in the bioreactor is minimized, the circulation of the culture liquid and the like is smooth, and the efficiency of the biological process can be improved by minimizing the residual of the final product in which the biological process is completed.

In addition, the disposable container of the bioreactor can be folded so that the space occupied by the disposable container of the bioreactor during transportation or storage can be minimized.

1 is an exploded perspective view of a disposable container of a bioreactor according to a first embodiment of the present invention;
Fig. 2 is a longitudinal sectional view of the disposable container of the bioreactor shown in Fig. 1
3 is an enlarged view of a portion indicated by A in Fig.
Figs. 4 and 5 are views for explaining a modification of the portion shown in Fig. 3
6 is an exploded perspective view of the disposable container of the bioreactor according to the second embodiment of the present invention.
7 is a perspective view of the disposable container of the bioreactor shown in Fig. 6
8 is a longitudinal sectional view taken along line BB in Fig. 7
9 is an exploded perspective view of the disposable container of the bioreactor according to the third embodiment of the present invention.
FIG. 10 is a perspective view of the disposable container of the bioreactor according to the third embodiment of the present invention shown in FIG. 9
11 is a perspective view of a disposable container of a bioreactor according to a fourth embodiment of the present invention
12 is a perspective view of a disposable container of a bioreactor according to a fifth embodiment of the present invention
13 is a longitudinal sectional view along CC line segment of Fig.
14 is an exploded perspective view of the disposable container of the bioreactor according to the sixth embodiment of the present invention
Fig. 15 is a view for explaining the fusing method of the cover and the base shown in Fig. 14
16 is a longitudinal sectional enlarged view of a portion indicated by D in Fig.
17 is a view for explaining a modification of the portion shown in Fig. 16
Fig. 18 is a perspective view of the disposable container of the bioreactor shown in Fig. 14
19 is a view illustrating a state in which the disposable container of the bioreactor shown in Fig. 18 is folded
20 is an exploded perspective view of the disposable container of the bioreactor according to the seventh embodiment of the present invention
Figs. 21 to 24 are views for explaining the fusing method of the cover and the base shown in Fig. 20
25 is an exploded perspective view of the disposable container of the bioreactor according to the eighth embodiment of the present invention
Fig. 26 is a view for explaining a fusing method of the cover and the connecting ring shown in Fig. 25
Fig. 27 is an enlarged longitudinal sectional view of a portion indicated by E in Fig.
28 is an exploded perspective view of the disposable container of the bioreactor according to the ninth embodiment of the present invention
29 is a longitudinal sectional enlarged view of a portion indicated by F in Fig.
30 is a flowchart for explaining a method of manufacturing a disposable container of a bioreactor according to an embodiment of the present invention.

The present invention is capable of various modifications and various embodiments, and specific embodiments are illustrated and described in the drawings. It is to be understood, however, that the invention is not to be limited to the specific embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

In the present specification, as described above, a system for artificially reproducing biochemical reaction processes such as decomposition, synthesis, and chemical transformation of substances in the body of a living organism, as well as a mixer for producing a cell culture medium or a culture solution used for cell culture, Will be collectively referred to as a bioreactor.

For convenience of explanation, the disposable container of the bioreactor will be abbreviated as a disposable container in the detailed description of the present specification, and the description of the disposable container to be described below may be applied to various types of bioreactor The culturing operation will be described as an example.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Fig. 1 is an exploded perspective view of a disposable container 100 according to a first embodiment of the present invention, and Fig. 2 is a longitudinal sectional view of the disposable container 100 shown in Fig.

Referring to FIGS. 1 and 2, the disposable container 100 according to the first embodiment of the present invention includes a cover 110, a base 130, and an agitator 150.

The cover 110 is made of at least one structural layer having a flexible layer and has a cylindrical shape with an open bottom. The cover 110 has flexibility. The cover 110 will be described below with reference to FIG.

The base 130 has a cylindrical shape with an opened upper surface and at least one connection port 131 protruding outward is formed on a side surface. The connection port 131 is formed to have a tubular shape penetrating to the inside of the base 130 along its longitudinal direction.

The connection port 131 is a pipe for supplying oxygen and nutrients to the disposable container 100, a channel for circulating the culture solution, a channel for harvesting the cultured cells, oxygen saturation and temperature of the culture solution Sensor, etc., are connected to each other.

Therefore, although not shown in detail, fastening grooves, fastening protrusions, threads, etc. may be formed on the outer circumferential surface or the outer end of the connection port 131 to facilitate connection of other conduits, devices, and the like. In this case, a rib that can be fixed by a clamp or the like may be formed.

The base 130 should be made of a material that does not affect the operation such as cell culture or contamination of the material because it is in direct contact with the material such as the culture solution and the cultured cells contained in the disposable container 100. Examples of such a material include a polyethylene resin having a purity of 99.99% or more.

A fixing protrusion 132 is formed on an inner bottom surface of the base 130 so that the stirrer 150 can be seated at a predetermined position. Alternatively, a fixing groove may be formed instead of the fixing protrusion 132 (not shown), and a fixing groove or fixing protrusion corresponding to the fixing protrusion 132 or the fixing groove may be formed on the bottom surface of the stirrer 150.

The stirrer 150 is configured to circulate a culture liquid or the like contained in the container 100 when a cell culture or the like is performed to uniformly disperse oxygen and nutrients and to uniformly distribute the temperature within the container 100 Can be used.

The agitator 150 includes a base 155 fixed at a predetermined position by a fixing protrusion 132 and having a rotation axis (not shown), a stirring main body 151 rotatably coupled to the rotation axis, A plurality of stirring vanes 153 coupled to the stirring main body 151 and a stirrer driving means 160 for transmitting rotation force to the stirring main body 151 to rotate in a predetermined direction.

Although not shown in detail, a plurality of permanent magnets are built in the stirring main body 151, and N poles or S poles are disposed so as to face the magnetic force driving means 160. At this time, the plurality of permanent magnets may be arranged such that N poles or S poles of the neighboring magnets are alternately arranged.

A number of electromagnets corresponding to permanent magnets (not shown) are built in the magnetic force driving means 160 so that the stirring main body 151 is rotated or stopped in a desired direction by adjusting the direction of current supplied to the plurality of electromagnets And the rotational speed can also be adjusted.

The stirrer 150 of the type in which the stirring main body 151 is driven by the magnetic force does not cause any contamination inside the container 100 because the inside and outside of the container 100 are blocked by the base 130 Effect can be obtained.

For this, the stirrer 150 is also in direct contact with the material contained in the disposable container 100 like the base 130, so that the stirrer 150 should be made of a material that does not cause contamination upon contact with such material.

For reference, although not shown, when the cells to be cultured are sensitive to magnetic force, the stirring driving means 360 may be disposed outside the cover 310 as shown in FIG.

On the other hand, the base 130 is formed to have sufficient rigidity (stiffness) unlike the cover 110 having flexibility. This facilitates the self-standing of the disposable container 100 by the base 130 and simplifies the structure of the supporting structure (not shown) for supporting the cover 110 to simplify the structure of the bioreactor using the disposable container 100 It is possible to save the cost and time required.

The base 130 may have a sufficient stiffness in consideration of the load applied to the base 130 according to the capacity of the disposable container 100 and the stiffness may be adjusted by adjusting the thickness of the wall of the base 130 Method or the like.

Meanwhile, when the disposable container 100 is stored or transported before being used, the flexible cover 110 is folded and accommodated in the base 130, so that the space occupied by the disposable container 100 can be minimized have.

At this time, a plurality of connecting wires having appropriate radiuses of curvature may be previously formed on the cover 110 so that the cover 110 is not damaged during irregular folding, or the side surface of the cover 110 may have a round- have. This will be described below with reference to Figs. 14 to 19.

The base 130 is coupled to the open lower side of the cover 100 as shown in FIG. That is, the lower end of the cover 110 and the upper end of the edge of the base 130 are engaged.

The cover 110 and the base 130 may be joined by various methods such as fusion bonding, adhesion, mechanical coupling by a coupling means such as a clamp, or the like. However, as described above, the material accommodated in the disposable container 100 should not affect the contact between the cover 110 and the base 130.

Therefore, as for the method of bonding the cover 110 and the base 130, fusion bonding or mechanical bonding may be advantageous in chemical or biological aspects. At this time, there is a disadvantage that the weight of the disposable container 100 is increased and the number of parts is increased because the coupling means described above must be additionally used in the method of mechanical coupling.

Therefore, in this specification, it is assumed that the coupling of the cover 110 and the base 130 is achieved by welding.

The lower end of the structural layer constituting the cover 110 is fused to the upper end of the edge of the base 130 so that the cover 110 and the base 130 are completely closed. The coupling will be described with reference to FIG.

Fig. 3 is an enlarged view of a portion indicated by A in Fig.

Referring to FIG. 3, the cover 110 includes a first structure layer 111, a second structure layer 113, and a third structure layer 115. Coupling grooves 112, 114, and 116 are formed in the first structure layer 111, the second structure layer 113, and the third structure layer 115, respectively.

Here, the first structural layer 111 is made of a material which does not cause contamination or the like upon contact with the material contained in the disposable container 100, as in the case of the base 130.

The second structure layer 113 assumes a case where a cell sensitive to concentration such as oxygen and carbon dioxide is cultured with a bioreactor (not shown) provided with a disposable container 100, And to prevent gas such as oxygen or carbon dioxide from permeating through the material when the material has fine pores.

For this purpose, the second structure layer 113 may be made of a material having a low gas permeability and act as a barrier.

The third structure layer 115 assumes a case where the capacity of the disposable container 100 is large and when the force due to the pressure applied to the cover 110 by the material accommodated in the cover 110 is large, May be made of a material having a high tensile strength such as nylon (polyamide) in order to prevent rupture of the material.

Accordingly, the cover 110 necessarily includes the first structural layer 111, while the second structural layer 113 and the third structural layer 115 can be selectively applied as needed. In addition, the arrangement order of the second structure layer 113 and the third structure layer 115 as described above can be changed as needed.

Although not shown, when the material accommodated in the disposable container 100 is sensitive to ultraviolet rays or visible light, it is necessary to use a material that is shielded from ultraviolet rays or visible rays in addition to the above-described structural layers 111, 113, and 115 More structure layers can be added. The plurality of structural layers 111, 113, and 115 may be fused to each other or may not be fused, but may be used in a state in which they are in close contact with each other or a state in which they are bonded to each other by a pressure-sensitive adhesive or an adhesive.

In this embodiment, it is assumed that the cover 110 has three structure layers 111, 113, and 115 as shown in the following description.

As shown in FIG. 3, the upper end of the base 130 has three engagement protrusions corresponding to the number of the structural layers 111, 113, and 115 along the outer circumference. And may be sequentially formed toward the outward direction so as to be arranged in the downward direction. Alternatively, although not shown, the latching jaws may be formed so as to be sequentially disposed toward the inner side of the base 130 as needed.

Engagement projections 135, 136, and 137, respectively corresponding to the engaging grooves 112, 114, and 116, respectively, are formed in the portions of the structural layer 111, 113, and 115 where the engaging jaws are formed, . Although not shown, a coupling protrusion may be formed on the structure layers 111, 113, and 115 as needed, and a coupling groove may be formed on the coupling protrusion.

After the coupling grooves 112 of the first structure layer 111 are coupled to the coupling protrusions 135, the ends of the first structure layer 111 are heated by the heating means or the ultrasonic wave generating means, So that the fused portion W is formed.

At this time, the fused portion W is made to have a sufficient width so as to have sufficient strength so that even if a substance such as a culture liquid is contained in the disposable container 100, the fusion-bonded portion W is not ruptured.

Hereinafter, the heating means or the ultrasonic wave generating means for forming the fused portion W will be referred to as fusing means as described above.

The engagement of the coupling groove 112 and the coupling projection 135 may be achieved by maintaining the temporarily fixed state of the first structure layer 111 and the coupling groove 112 to be fused by using a heating means or an ultrasonic wave generating means And to prevent the fused portion W from being unevenly formed by wrinkling of the first structural layer 111 or the like.

The adhesive grooves 112 and the engaging protrusions 135 can be replaced with adhesives or adhesives. The adhesive or the adhesive can be applied to the lower side of the fused portion W, that is, the end portion of the first structural layer 111 After the fused portion W is formed, the contents of the disposable container 100 such as a culture liquid can be prevented from contacting the adhered or adhered portion.

Although not shown in the drawings, the fused portions are formed in the second structure layer 113 and the third structure layer 115 as in the case where the fused portion W is formed in the first structure layer 111, Thereby making it possible to firmly engage the protrusions 130.

Accordingly, after the cover 110 and the base 130 are coupled as described above, the inside of the disposable container 100 can be sealed off from the outside and completely shut off. Therefore, the disposable container 100 can be used for cell culture The operation can be performed safely.

Referring again to FIG. 2, a rounded portion 134 is formed at an inner lower edge of the base 130.

The rounding part 134 is provided to prevent a dead zone, which is not partially circulated in the process of circulating the material contained in the disposable container 100 by the operation of the stirrer 150, will be. Here, the above-mentioned dead zone refers to a portion where a vortex or a vortex is formed locally to circulate the material only in a specific region of the disposable container 100.

In the conventional disposable container mentioned above, the outer wall of the disposable container is wrinkled or wrinkled at the edge portion of the bottom surface, and such a portion frequently acts as a dead zone.

When the above-described dead zone is formed in the disposable container 100, there is a high possibility that waste products, dead cells, foreign matter, and the like generated during work such as cell culture accumulate in the dead zone. In this case, as time elapses, accumulated substances may be oxidized, deteriorated, and / or decayed, which may result in the deterioration or contamination of the entire material contained in the disposable container 100.

In addition, when the final product is discharged to the outside of the disposable container 100 after the biological process is completed, the accumulated substances in the dead zone are not discharged smoothly and are likely to remain in the disposable container 100.

Particularly, when corners are formed between the inner bottom surface and the side surface of the disposable container, there is a problem that the cells being cultured are destroyed by colliding with the culture medium while flowing along with the culture liquid, thereby lowering the yield.

Therefore, by forming the rounding portion 134 in the base 130 to prevent formation of a dead zone, stability and yield of operations such as cell culture using the disposable container 100 can be improved.

The rounding portion 134 may be formed at a lower edge portion of the base 130 as shown in the figure so that the material contained in the disposable container 100 can be shaped to flow smoothly along the rounding portion 134 .

In general, the rounding part 134 may have a curved shape connecting the bottom surface and the side surface inside the base 130. Physical characteristics such as the radius of curvature and the surface roughness of the rounding part 134 may be determined through experiments and simulations Can be derived.

For reference, although not shown, the rounding portion 134 may be formed on the entire bottom surface of the base 130 except the portion where the stirrer 150 is seated, or may be formed such that the radius of curvature is continuously changed .

In addition, although not shown, the rounding part 134 includes a plurality of connection ports 131, which are connected to a drain line for discharging wastes generated in a biological process to the outside of the disposable container 100, Or the harvest line for collecting the final product after the biological process is completed is inclined downward toward the port to which it is connected so that the contents in the disposable container 100 can be readily transferred through all the drain lines or collection lines And may be formed to be discharged.

Fig. 4 and Fig. 5 are views for explaining a modification of the portion shown in Fig. Here, it is also assumed that the cover 110 is made up of three structure layers 111a, 113a, and 115a.

4 and 5, a number of insertion grooves 135a, 136a, and 137a corresponding to the number of the structural layers 111a, 113a, and 115a is formed along the outer circumference at the upper end of the edge of the base 130a .

The first structure layer 111a, the second structure layer 113a, and the third structure layer 115a are inserted into the insertion grooves 135a, 136a, and 137a, respectively, as shown in FIG. Support ribs 135b, 136b, and 137b are protruded from the insertion grooves 135a, 136a, and 137a to prevent the inserted structure layers 111a, 113a, and 115a from being separated from the structure layers 111a, As shown in Fig.

In this modified example, the fused portions Wa, Wb and Wc are sequentially formed on the contact surfaces of the base 130a and the structural layers 111a, 113a and 115a, as described with reference to Fig. 3, , 113a, and 115a are coupled to the base 130a.

This modification is for easily forming the fused portions Wa, Wb, Wc by temporarily fixing the structural layers 111a, 113a, 115a to the insertion grooves 135a, 136a, 137a.

1 and 2, the cover 110 has a cylindrical shape, that is, a cylindrical shape with a bottom surface opened as described above.

Although not shown, the cover 110 can be manufactured in various shapes as needed. For example, it may be formed to have a shape such as an elliptical pole, a prism, a cone, a cone, or a pyramid in addition to a cylindrical shape. Accordingly, the shape of the open bottom surface of the cover 110 may have an ellipse, polygon, or the like other than a circle. That is, the cover 110 is formed in the shape of a container having an open bottom and various shapes.

Accordingly, the outer shape of the open upper surface of the base 130 may have a shape such as a circle, an ellipse, or a polygon corresponding thereto. That is, the upper surface of the base 130 also has the shape of a container having various shapes.

FIG. 6 is an exploded perspective view of the disposable container according to the second embodiment of the present invention, FIG. 7 is a perspective view of the disposable container shown in FIG. 6, and FIG. Respectively.

6 and 7, the disposable container 200 according to the second embodiment of the present invention includes a cover 210, a base 230, a cover temperature adjusting unit 240, a stirrer 250, Means 280 are included.

Since the cover 210, the base 230 and the stirrer 250 are the same in structure and function as the cover 110, the base 130 and the stirrer 250 described above, a repetitive description will be omitted.

A support protrusion 238 protrudes from the outer circumferential surface of the base 230 on the upper side of the connection port 231. The support protrusion 238 is formed on the outer surface of the base 230 so that the cover temperature adjusting means 240, The cover temperature regulating means 240 is supported by the base 230. [0050]

The inner surface of the hollow portion of the cover temperature adjusting means 240 has a shape corresponding to the outer circumferential surface of the cover 210 so that the cover 210 As shown in FIG.

8, a heat medium circulation channel 241 is formed inside the cover temperature regulating means 240 and heat medium circulation ports 242 and 243 are formed on the outer circumferential surface thereof so as to be in communication with the heat medium circulation channel 241 .

Although not shown, heat medium such as hot water or cold water flows into or out of the heat medium circulation ports 242 and 243 so that the heat medium circulates the heat medium circulation flow path 241 and heat of the heat medium is transmitted through the outer peripheral surface of the cover 210 The temperature of the material contained in the disposable container 200 can be adjusted or kept constant.

The base temperature adjusting means 280 is formed to be coupled to the base 230 to surround the side surface and the bottom surface of the base 230 as shown in FIGS.

At this time, a driving means inserting port 285 is formed at an intermediate portion of the base temperature regulating means 280 so that the stirring driving means 260 is inserted into the stirrer 250 so as to be as close as possible to the stirrer 250 do.

6 and 7, a port groove 284 is formed at an edge portion of the base temperature adjusting means 280. This is because the base temperature adjusting means 280 is formed at the edge of the base temperature adjusting means 280, So that the edge portion is not interfered with the connection port 231.

8, the heat medium circulation flow path 281 is formed in the base temperature adjusting means 280 and the heat medium circulation ports 282 and 283 are formed on the outer circumferential surface thereof so as to be in communication with the heat medium circulation flow path 281 .

The heat medium is introduced into or discharged from the heat medium circulation ports 282 and 283 so that the heat medium circulates through the heat medium circulation flow path 281 to transfer heat of the heat medium through the bottom surface and the inner surface of the base 230, The temperature of the material contained in the container 200 can be adjusted or kept constant.

For reference, the number of the heating medium circulation ports 242, 243, 282, and 283 may be increased or decreased as necessary in consideration of the capacity of the disposable container 200 and the precision of temperature control.

FIG. 9 is an exploded perspective view of a disposable container 300 according to a third embodiment of the present invention, and FIG. 10 is a perspective view of the disposable container 300 according to the third embodiment of the present invention shown in FIG. Respectively.

Referring to FIGS. 9 and 10, the disposable container 300 according to the third embodiment of the present invention includes a cover 310, a base 330, a stirrer 350, and a cap 370.

The base 330 has a connection port 331, which is the same in structure and function as the base 130 and the connection port 131 described above, so that duplicate descriptions are omitted.

The cover 310 is formed of one or more structural layers as described above, and a cap coupling hole 317 is formed in a part of the upper surface of the cover 310.

The agitator 350 includes a stirring main body 351, a stirring vane 353, a stirring driving means 360 and a stirring shaft 361. The stirring driving means 360 is a device for generating a rotating force like an electric motor and rotates the stirring shaft 361 connected to the stirring driving means 360 at one side. The agitating main body 351 is provided with a stirring vane 353 radially and an intermediate portion of the agitating main body 351 is formed with a shaft coupling hole 352 to which the other end of the agitating shaft 361 is coupled.

The cap 370 is provided with a shaft hole 371, an inlet port 372, a plurality of fastening ports 373, and a coupling portion 374.

The shaft hole 371 rotatably supports the stirring shaft 361 while the shaft 371 is rotatably supported by the disposable container 300, And a sealing means for blocking entrance to and exit from the inside and outside is provided.

The inlet port 372 is a portion used when a large amount of material is put into the culture liquid etc. during the operation of cell culture or the like to the disposable container 300 and the fastening port 373 is a portion for introducing the gas generated in the disposable container 300 And to connect a vent pipe or a water level sensor to be discharged to the outside.

The joining portion 374 may be formed so that the edge portion of the cap coupling hole 317 and the cap 370 are fusion-bonded to each other so as to be hermetically sealed by the method described with reference to Figs. 3 and 4 to form the fused portion Wd (See 135, 136 and 137 in FIG. 3) or insertion grooves 135a, 136a, and 137a may also be formed in the engaging portion 374. As shown in FIG.

In the disposable container 300 according to the third embodiment of the present invention, a so-called 'top drive system' in which the stirring driving means 360 is disposed on the upper side is applied. As described above, when the stirrer 350 is driven It can be applied to the case where it is difficult to perform. For example, the present embodiment can be applied when the material or the cells contained in the disposable container 300 have a low yield when magnetic force is applied thereto.

For reference, the cap 370 may also be formed to have sufficient stiffness as the base 330.

FIG. 11 is a perspective view of a disposable container 400 according to a fourth embodiment of the present invention.

Referring to FIG. 11, a disposable container 400 according to a fourth embodiment of the present invention includes a cover 410, a base 430, a stirrer 450, and a cap 470.

The connection port 331 is formed in the base 430. Since the structure and operation of the connection port 331 are the same as those of the base 130 and the connection port 131 described above, a duplicate description will be omitted.

The cover 410 is formed of one or more structural layers as described above, and the cover 410 has an entire upper surface opened so that the cover 410 has a cylindrical shape in which both the upper surface and the lower surface are opened. That is, the cover 410 has a structure in which the cap coupling hole 317 is extended to the entire upper surface of the cover 410 in the cover 310 described above.

The stirrer 450 includes the stirrer driving means 460 and the stirring shaft 461. Since the structure and operation of the stirrer 450 are the same as those of the stirrer 350 described above, a duplicate description will be omitted.

The cap 470 may have a cylindrical shape with an opened lower surface. The cap 470 is coupled to the open upper side of the cover 410. The upper end of the cover 410 and the lower end of the edge of the cap 470 are joined together by the fusion welding method described above. That is, the cover 410 and the cap 470 are coupled in the same manner as the cover 410 and the base 430 are coupled together, and thus the description thereof will be omitted.

The shaft hole 471 and the inlet port 472 and the plurality of fastening ports 473 formed in the cap 470 have the same structure and functions as those of the shaft hole 371, the inlet port 372 and the fastening port 373 described above. .

The disposable container 400 according to the fourth embodiment of the present invention assumes the case where the capacity is considerably large in order to produce a large amount of product so that the cover 410 is more firmly supported by the cap 470 And the structural strength of the disposable container 400 is increased.

12 is a perspective view of a disposable container according to a fifth embodiment of the present invention, and FIG. 13 is a longitudinal sectional view taken along line C-C of FIG.

Referring to FIGS. 12 and 13, the disposable container 500 according to the fifth embodiment of the present invention includes a cover 510 and a base 530.

The cover 510 is composed of one or more structural layers similarly to the cover 110 described above.

A plurality of connection ports 531 and a plurality of heat medium circulation ports 537 and 538 are formed on the outer circumferential surface of the base 530. 13, a heat medium circulation flow path 536 is formed in the base 530. [

That is, the base 530 circulates through the heat medium circulation flow path 536 through the heat medium circulation ports 537 and 538, and the heat medium is circulated through the heat medium circulation path 536 through the inner side surface of the base 530 The temperature can be kept constant or adjusted by being transferred to the material.

A fixing protrusion 532 is formed on the inner bottom surface of the base 530, which is the same in structure and function as the fixing protrusion 132 of FIG.

The driving means insertion port 535 formed on the outer bottom surface of the base 530 is connected to the fixing projection 532 by stirring driving means (see 260 in FIG. 8) using magnetic force like the above-described driving means insertion port (285 of FIG. 8) (Refer to 150 in Fig. 1) that is placed on the top of the agitator.

Meanwhile, in the disposable container 500 according to the present embodiment, the height HB of the base 530 is formed to be significantly higher than the height HC of the cover 510. This allows the base 530 having a high rigidity to support most of the load caused by the material contained in the disposable container 500 so that a high structural strength can be obtained when the size of the disposable container 500 is large.

Although not shown, the connection port 531 is formed so as to pass through from the side to the inside of the side of the base 530, as described above, but is not formed to be in a state of being not in communication with the heat medium circulation channel 536.

14, an exploded perspective view 600 of a disposable container according to a sixth embodiment of the present invention is shown.

Referring to FIG. 14, the disposable container 600 according to the sixth embodiment of the present invention includes a cover 610, a base 630, a stirrer 650, and a cap 670.

The cover 610, like the cover 410 described above, is formed of at least one structure layer and has a cylindrical shape with both the top and bottom surfaces opened. At least one connecting wire 617 is formed on the side surface of the cover 610.

When the cover 610 is deformed by applying an external force in a specific direction to the cover 610, the portion of the connecting wire 617 is folded first so that the cover 610 deforms to a predictable shape .

For example, the connecting wire 617 may be formed by applying a mechanical force to a predetermined portion of the cover 610 to form a wrinkle.

A connection port 631 and a fixing protrusion 632 are formed on the base 630.

Since the structure and operation of the connection port 631 are the same as those of the connection port 131 of the base 130 described above, redundant description will be omitted.

The fixing protrusion 632 allows the stirrer 650 to be seated at a predetermined position on the inner bottom surface of the base 630 like the fixing protrusion 132 described above.

The agitator 650 includes a stirring main body 651 and a stirring blade 653. Here, the stirring main body 651 and the stirring vane 653 are the same in structure and function as the stirring main body 151 and the stirring vane 153 described above, and therefore, duplicated description will be omitted.

However, a shaft hole 652 is formed in the stirring main body 651, and the shaft hole 652 is formed so that the fixing protrusion 632 can be inserted. That is, the stirring main body 651 is rotatably coupled to the inner bottom surface of the base 630 with the fixing protrusion 632 as a rotation axis. Therefore, the fixing protrusion 632 is formed to have a shape capable of rotatably supporting the stirring main body 651.

On the other hand, after the stirring main body 651 formed on the fixing protrusion 632 is engaged with the fixing protrusion 632, it should be prevented from being arbitrarily disengaged. For this purpose, a seating rib 633 is formed to protrude from the outer periphery of the fixing protrusion 632, so that the stirring main body 651 can be rotatably supported. In the shaft hole 652, a locking step (not shown) corresponding to the seating rib 633 may be formed.

Although not shown, the outer circumferential surface of the fixing protrusion 632 may have a seating groove instead of the seating rib 633. The inner surface of the shaft hole 652 may have a seating rib corresponding to the shape and position of the seating groove . Although not shown, a bearing may be interposed between the outer circumferential surface of the fixing protrusion 632 and the inner circumferential surface of the shaft hole 652.

The rotation of the agitating main body 651 can be adjusted when the agitator driving means 160 is disposed below the base 630 by the above-described structure.

The cap 670 is provided with a charging port 672 and a plurality of fastening ports 673

The cap 670, the charging port 672 and the plurality of fastening ports 673 are the same in structure and function as the cap 470, the charging port 472 and the fastening port 473 described above.

Fig. 15 is a view for explaining the welding method of the cover 610 and the base 630 shown in Fig. 14, and Fig. 16 is an enlarged view of the longitudinal section of the portion indicated by D in Fig.

15 and 16, the lower end portion of the cover 610 is fused to the upper end portion of the base 630 by the fusing means 10.

The fusing unit 10 is exemplified by simplifying the means for causing the fusing head 11 to move to a desired position and applying heat or ultrasonic waves to fuse the object. The fusing unit 10 includes a fusing head 11, a fusing arm 13, and an actuator (not shown).

Although not shown in detail, the fusing head 11 has a built-in heater or an ultrasonic vibrator so that heat or ultrasonic waves can be emitted from the fusing head 11.

A fusing head 11 is coupled to one end of the fusing arm 13 and the other end of the fusing arm 13 is coupled to an actuator (not shown). Accordingly, the fusing head 11 can be moved to the desired position in accordance with the operation of the actuator (not shown).

Therefore, the fusing unit 10 can be made such that the outer peripheral surface of the lower end of the cover 610 is fused to the inner circumferential surface of the upper edge of the base 630, as shown in Fig.

That is, the cover 610 and the base 630 are relatively moved so that the fusion between the cover 610 and the base 630 is partially overlapped as shown in Fig. 16 so that the necking can be performed at a desired position Next, the fusing head 11 presses the overlapped portion with a predetermined force P and applies heat or ultrasonic waves.

As a result, the pressure of the cover 610 and the base 630 and the temperature of the portion where the heat or the ultrasonic wave is applied are increased, so that fusion is performed.

At this time, in order to prevent the cover 610 and the base 630 from being moved by the predetermined force P applied to the cover 610 and the base 630 to change their relative positions, A jig (not shown) for fixing the base 610 and the base 630 may be used.

In order for the fused portion between the cover 610 and the base 630 to have a constant strength, the overlapping width must be maintained constant as described above. For this purpose, the base 630 may be provided with a latching protrusion 638 on which the lower end of the cover 610 is seated.

Fig. 17 is a view for explaining a modification of the portion shown in Fig.

Referring to FIG. 17, the base 630a is formed with a latching protrusion 638a on which the lower end of the cover 610 is seated, and a protrusion 639a is formed in the latching protrusion 638a. The fusing head 11a is also formed with a corrugated portion 12 having a shape corresponding to the corrugated portion 639a of the engaging tab 638a.

The corrugated portion 12 of the fusing head 11a and the corrugated portion 639a of the base 630a are formed by a plurality of concave-convex portions as shown in the figure, and the cover 610 and the base 630a are fusion- So that the area of the fused portion per unit length is increased. As a result, the bonding strength of the fused portion is increased.

For reference, the above-described latching protrusions 638 and 638a may be formed on the inner circumferential surface of the bases 630 and 630a as shown or may be formed on the outer circumferential surfaces of the bases 630 and 630a, as shown in the figure. This can be selected in accordance with conditions such as ease of fusion welding of the cover 610 and the bases 630 and 630a and strength required for the fused portion.

The disposable container 600 is formed in such a manner that the stoppers 638 and 638a are formed on the inner peripheral surfaces of the bases 630 and 630a and the outer peripheral surface of the lower end of the cover 610 is fused to the inner peripheral surface of the bases 630 and 630a, A compressive force and a shearing force are exerted on the fused portion when a force is applied in a direction in which the cover 610 is expanded. Here, the shearing force refers to the force directed toward the cover 670 by the pressure in the disposable container 600 to the cover 610.

On the other hand, when the engaging protrusions 638 and 638a are formed on the outer peripheral surfaces of the bases 630 and 630a and the inner peripheral surface of the lower end of the cover 610 is fused to the outer peripheral surfaces of the bases 630 and 630a, When the pressure in the fused portion 600 is increased, a tensile force and a shearing force are applied to the fused portion.

Since the fused portion is a portion formed by pressing and cooling the cover 610 and portions of the surfaces of the bases 630 and 630a after melting, the durability against the compressive force is generally much higher than the durability against the tensile force. Therefore, the withstand pressure strength of the disposable container 600 is higher when the outer circumferential surface of the lower end of the cover 610 is fused to the inner circumferential surface of the bases 630 and 630a, and vice versa.

16 and 17, when the outer peripheral surface of the cover 610 is fused to the inner peripheral surfaces of the bases 630 and 630a, the fusing heads 11 and 11a of the fusing means 10 are fixed to the cover 610 In the direction toward the outside.

Therefore, the fusing arm 13 should be constructed to be able to move the fusing heads 11, 11a inside the cover 610, and a jig for supporting the force P to be pressed by the fusing heads 11, 11a There are disadvantages.

On the other hand, when the inner peripheral surface of the lower end portion of the cover 610 is fused to the outer peripheral surfaces of the bases 630 and 630a, the pressing force by the fusing heads 11 and 11a is supported by the bases 630 and 630a, And the fusing heads 13 and 11a are movable from the outside of the cover 610 and the bases 630 and 630a so that the structure of the fusing arm 13 becomes relatively simple.

Therefore, the fusion of the cover 610 and the bases 630 and 630a can be selected and applied in consideration of the pressure resistance performance required for the disposable container 600, the manufacturing cost, and the like.

Fig. 18 is a perspective view of the disposable container 600 shown in Fig. 14, and Fig. 19 is a view showing the disposable container shown in Fig. 18 being folded.

18, the cover 610, the base 630 and the cap 670 are coupled to each other, and the stirrer 650 is rotatably coupled to the base 630 to complete the disposable container 600.

The coupling of the cover 610 and the cap 670 may be achieved by coupling the upper end of the cover 610 and the lower end of the cap 670 by a method such as fusion described above. That is, the cover 610 and the cap 670 may be coupled to each other in such a manner that the cover 110 and the base 130 are coupled to each other or the cover 610 and the base 630 are coupled to each other. .

In order to perform various biological processes using the disposable container 600, sensors and pipelines necessary for the connection port 631 and the fastening port 673 may be connected to complete the bioreactor system and operate the same.

If the disposable container 600 is to be transported and stored, an external force may be applied in the direction indicated by an arrow in FIG. 19 to reduce the disposable container 600 occupied by the disposable container 600.

This is a modification to the external force applied in the direction of the arrow shown in Fig. 19, that is, in the vertical direction of the disposable container 600. [ That is, the portion of the cover 610 on which the connecting wire 617 is formed is folded by the above-described external force, and the cover 610 is deformed into a shape in which the length in the vertical direction, that is, the height is shortened.

At this time, when the folded state of the cover 610 is accommodated in the base 630 according to conditions such as the relative sizes of the base 630 and the cover 610 and the magnitude of the external force, the volume of the folded disposable container 600 Can be minimized.

Accordingly, when a plurality of disposable containers 600 are to be carried or stored, the cover 610 is folded as described above, thereby improving the space utilization efficiency and transportability.

20 is an exploded perspective view of the disposable container 700 according to the seventh embodiment of the present invention.

Referring to FIG. 20, the disposable container 700 according to the seventh embodiment of the present invention includes a cover 710 and a base 730.

The cover 710, like the cover 110 described above, is formed of at least one structural layer and has a cylindrical shape with an open bottom.

A connection port 731, a fixing protrusion 732, a locking protrusion 738 and a tension groove 739 are formed in the base 730.

Since the connection port 731 is the same in structure and function as the connection port 131 of the base 130 described above, a duplicate description will be omitted.

The fixing protrusion 732 allows the stirrer (not shown) (not shown) of the fixing protrusion 632 to be rotatably coupled to a predetermined position on the inner bottom surface of the base 730, similarly to the fixing protrusion 632 described above.

The latching jaw 738 is formed so that the lower end of the cover 710 can be seated like the latching jaw 638 described with reference to Fig. The engaging jaw 738 may be formed on the inner circumferential surface of the base 730 as required, such as the engaging jaw 638 described above.

The tension groove 739 is formed in a shape embedded in the outer peripheral surface between the engagement protrusion 738 and the upper end of the base 730, that is, the upper end of the edge of the base 730 and the lower end of the cover 710.

The tension grooves 739 have grooves extending in the direction parallel to the central axis of the base 730 or the longitudinal axis of the fixing protrusions 732, that is, long grooves. The number of tensile grooves 739 can be increased or decreased as needed from one to more than one. Further, when there are a plurality of tension grooves 739, it may be arranged along the outer periphery of the upper end of the base 730 as shown in the figure.

The tension grooves 739 prevent wrinkles that may form in the cover 710 in the process of engaging the cover 710 and the base 730 so that the cover 710 and the base 730 are firmly coupled So as to improve the hermeticity.

The function of the tensile groove 739 will be described with reference to FIGS. 21 to 24. FIG.

Figs. 21 to 24 are views for explaining the fusing method of the cover 710 and the base 730 shown in Fig.

Referring to Fig. 21, a top view of base 730 is shown. At this time, a plurality of tension grooves 739 are disposed along the outer periphery of the upper end of the base 730 as shown.

Referring to FIG. 22, a cover 710 is disposed outside the base 730. At this time, the lower end of the cover 710 and the upper end of the base 730 are disposed so as to partially overlap with each other by the portion to be formed with the fused portion, as described with reference to Fig.

The fusing unit 20 includes a plurality of fusing heads 21 and 25, a plurality of fusing arms 23 and 27 and a plurality of actuators (not shown) connected to the fusing arms 23 and 27, respectively. Therefore, the fusing unit 20 can cause the plurality of fusing heads 21 and 25 to be operated respectively.

At this time, among the plurality of fusing heads 21 and 25, the tension projections 22 corresponding to the number, shape and position of the tension grooves 739 are arranged corresponding to the portions where the tension grooves 739 are disposed .

Although not shown, a heater or an ultrasonic vibrator or the like is incorporated in the plurality of fusing heads 21 and 25 in the same manner as the fusing head 11 described above.

When the inner circumference of the lower end portion of the cover 710 and the outer circumference of the upper end of the base 730 are fused to each other, the inner diameter of the cover 710, As shown in FIG.

This is because the clearance is formed between the lower end of the cover 710 and the upper end of the base 730 so that they can be easily overlapped.

On the other hand, unlike the illustrated case, when the outer peripheral surface of the lower end portion of the cover 710 and the inner peripheral surface of the upper end portion of the base 730 are fused, the outer diameter of the cover 710 is smaller than that of the engagement protrusion 738 The inner diameter of the formed portion may be shorter than the inner diameter of the formed portion by a predetermined length.

This is also for the purpose of forming a clearance between the lower end of the cover 710 and the upper end of the base 730.

However, when the outer peripheral surface of the lower end of the cover 710 and the inner peripheral surface of the upper end of the base 730 are fused, the tension grooves 739 are formed in the inner peripheral surface of the upper end of the base 730.

23, the inner peripheral surface of the lower end of the cover 710 is pressed against the outer peripheral surface of the upper end of the base 730 by the fusing head 21 as the fusing head 21 is moved in the direction indicated by the dotted arrow.

At this time, the fusing head 21 applies a predetermined force (see P in FIG. 16) so as to press the lower end portion of the cover 710 and the upper end portion of the base 730 and simultaneously applies heat or ultrasonic waves to the cover 710 and the base 730, so that they are welded together.

In this process, the tension projection 22 of the fusing head 21 is inserted into the tension groove 739 of the base 730. As a result, the lower end of the cover 710 is press-fitted into the tension groove 739 as shown in FIG. Therefore, the entire inner peripheral surface of the lower end of the cover 710 is brought into close contact with the outer peripheral surface of the upper end of the base 730.

At this time, the degree of clearance can be adjusted according to the depth and the number of the tension grooves 739. That is, as the depth of the tensile grooves 739 is large and the number of the tensile grooves 739 is large, the clearance is removed as well as a little tensile stress is applied to the lower end of the cover 710. In this way, it is possible to prevent the cover 710 from being wrinkled during the fusion process of the cover 710 and the base 730.

However, when the tensile stress is excessively generated at the lower end of the cover 710, the thickness of the cover 710 may be reduced, and the tensile stress may remain after the fusion is completed to adversely affect the strength of the fused portion. The depth and the number of the tension grooves 739 are appropriately adjusted.

24, after the welding of the cover 710 and the part of the base 730 by the fusing head 21 is completed, the fusing head 21 is moved to be detached from the cover 710 and the base 730 Next, the fusing head 25 is operated so that the remaining portions are fused so that the portions of the cover 710 and the base 730 that are not fused are fused as shown in the figure.

Therefore, the fusing heads 21 and 25 are formed so as to fuse the entire overlapped portion of the cover 710 and the base 730 as shown in the figure, and the fusing heads 21 and 25 are formed so as not to form the non- 25 may be formed so as to overlap each other.

When the depth and the number of the tensile grooves 739 are increased as described above, the length of the portion where the cover 710 and the base 730 are fused, that is, the total area of the fused portion is proportionally increased. As the area of the fused portion is increased, the bond strength between the cover 710 and the base 730 is increased. Therefore, the depth and the number of the tensile grooves 739 can be appropriately increased or decreased according to the withstand pressure performance required for the disposable container 700.

The cover 710 and the base 730 shown in FIG. 20 can be coupled to each other in the manner described with reference to FIGS. 21 to 24. FIG.

25 is an exploded perspective view of a disposable container 800 according to an eighth embodiment of the present invention.

Referring to FIG. 25, the disposable container 800 according to the eighth embodiment of the present invention includes a cover 810, a connecting ring 820, and a base 830.

The cover 810, like the cover 110 described above, is formed of at least one structural layer and has a cylindrical shape with an open bottom.

A connection port 831 and a fixing protrusion 832 are formed in the base 830.

Since the connection port 831 has the same structure and function as the connection port 131 of the base 130 described above, a duplicate description will be omitted. The fixing protrusion 832 allows the stirrer (see 650 in FIG. 14), not shown, to be rotatably coupled to a predetermined position on the inner bottom surface of the base 830, similarly to the fixing protrusion 632 described above.

The connection ring 820 is disposed between the cover 810 and the base 830. That is, the connecting ring 820 is interposed in the cover 810 and the base 830.

Fig. 26 is a view for explaining a fusing method of the cover 810 and the connecting ring 820 shown in Fig. 25, and Fig. 27 is an enlarged view of the longitudinal section of the portion indicated by E in Fig.

26, the fusing unit 30 includes a fusing head 31, a fusing arm 33, and an actuator (not shown), which are attached to the fusing head 11, the fusing arm 13, (Not shown), the description thereof is omitted.

The outer peripheral surface of the lower end portion of the cover 810 and the inner peripheral surface of the connection ring 820 are fused by the fusing means 30. [ That is, after the outer peripheral surface of the lower end portion of the cover 810 and the inner peripheral surface of the connection ring 820 are partially overlapped with each other, the fusing means 30 is operated to press them with a predetermined force P, Or ultrasonic waves are applied so that they are welded to each other.

At this time, a locking protrusion 829 may be formed on the inner circumferential surface of the connection ring 820 so that the lower end of the cover 810 is easily seated.

26, the cover 810 and the connecting ring 820 can be joined by fusing as described above.

For reference, unlike the illustrated case, if necessary, the inner circumferential surface of the lower end of the cover 810 may be fused to the outer circumferential surface of the connecting ring 820.

A fastening protrusion 828 is formed on the lower side of the connection ring 820 and a fastening groove 838 corresponding to the fastening protrusion 828 is formed on the upper end of the base 830, And the base 830 can be fastened.

At this time, the fastening protrusion 828 and the fastening groove 838 may be fastened to each other by an interference fit tight fit to improve the sealing between the connecting ring 820 and the base 830, A sealing member (not shown) may be further interposed between the protrusion 828 and the fastening groove 838.

Although not shown, a coupling groove may be formed on the lower side of the coupling ring 820, and a corresponding coupling protrusion may be formed on the base 830, if necessary.

In the case where the connecting ring 820 is interposed between the cover 810 and the base 830 as described above, the path through which the fusing head 31 is to be moved for fusing can be simplified, .

Accordingly, the cost and time required for the fusing unit 30 can be saved, and the time and effort required for the fusing operation can be saved.

For reference, the shape of the lower end of the cover 810 and the upper end of the base 830 can be changed into various shapes as described above. Therefore, the shapes of the connecting ring 820 and the fusing head 31 can also be changed to corresponding shapes.

28 is an exploded perspective view of a disposable container of a bioreactor according to a ninth embodiment of the present invention, and FIG. 29 is an enlarged view of a longitudinal section of a portion indicated by F in FIG. 28

Referring to Figs. 28 and 29, a disposable container 900 according to a ninth embodiment of the present invention includes a cover 910, a reinforcing ring 920, and a base 930. Fig.

The cover 910, like the cover 110 described above, is formed of at least one structural layer and has a cylindrical shape with an open bottom.

A connection port 931 and a fixing protrusion 932 are formed in the base 930.

Since the connection port 931 is the same in structure and function as the connection port 131 of the base 130 described above, a duplicate description will be omitted. The fixing protrusion 932 allows the stirrer (see 650 in FIG. 14), not shown, to be rotatably coupled to a predetermined position of the inner bottom surface of the base 930, similarly to the fixing protrusion 632 described above.

The reinforcing ring 920 is formed to have a ring shape as shown in the figure and the inner peripheral surface of the reinforcing ring 920 is fused to the outer peripheral surface of the lower end of the cover 910 to form a fused portion W1. At this time, the fusing means 30 described above can be used for fusion between the cover 910 and the reinforcing ring 920.

A fastening protrusion 928 protrudes downward from the bottom of the reinforcing ring 920 and a fastening groove 938 corresponding to the fastening protrusion 928 is formed at an upper end of the base of the base 930. Although not shown, if necessary, a locking groove may be formed in the reinforcing ring 920, and a fastening protrusion corresponding to the locking groove may be formed at the upper end of the edge of the base 930.

The lower end of the cover 910 is brought into contact with the upper side of the base 930 when the fastening protrusion 928 and the fastening groove 938 are engaged with each other, that is, when the reinforcing ring 920 is seated on the upper side of the base 930. At this time, a coupling rib 939 is protruded from the base 930 as shown in the figure, so that the lower side of the cover 910 can be interposed between the inner peripheral surface of the reinforcing ring and the outer peripheral surface of the coupling rib.

At this time, the engaging rib 939 is formed so as to protrude further upward than the reinforcing ring 920, and a portion of the outer circumferential surface of the engaging rib 939 disposed above the reinforcing ring 920 and an inner circumferential surface of the cover 910 When the fused portion W2 is formed, the cover 910 and the base 930 are engaged.

Fusing between the cover 910 and the base 930 may be performed using the fusing unit 20 described above and provided with the fusing head 25 and the fusing arm 27.

Since the lower end of the cover 910 having flexibility is primarily fixed by the reinforcing ring 920, the disposable container 900 having such a configuration can be easily fixed to the upper end of the base 930 .

When the fused portion W2 is formed by fusing between the cover 910 and the base 930, the lower end of the cover 910 is fixed by the reinforcing ring 920, and fusing means, not shown, And the base 930, the fusion bonding work for forming the fusion bonding portion W2 can be facilitated and the inspection of the fusion bonding portion W2 can be easily performed.

The disposable container 900 is supported by the engaging rib 939 and the reinforcing ring 920 by a force applied to the side surface of the cover 910 by the pressure generated in the disposable container 900, It is possible to obtain an effect of improving the withstand pressure performance of the fuel cell.

30 is a flowchart illustrating a method of manufacturing disposable containers 100 to 800 according to an embodiment of the present invention.

30, a method of manufacturing a disposable container 100 to 800 according to an embodiment of the present invention includes a rigid body manufacturing step S10, a cover making step S20, a combining step S30, and a sterilizing step S40, .

The rigid body fabrication step S10 may be performed by a method such as injection or the like by using components having high stiffness such as the bases 130 to 830, the stirrers 150 to 650, the caps 370, 470 and 670 and the connecting ring 820 &Lt; / RTI >

The cover making step S20 refers to a step of manufacturing the cover 110 to 810 having flexibility.

The combining step S30 is performed by combining the components manufactured in the above-described rigid body part manufacturing step S10 and the cover manufacturing step S20 using the fusing means 10, 20 and 30 described above, 800 < / RTI >

The sterilizing step S40 refers to the step of sterilizing the disposable containers 100 to 800 formed through the combining step S30 by irradiating gamma rays or the like so that no foreign matter or bacteria remain.

Although not shown, the disposable containers 100 to 800 that have undergone the sterilization step S40 are transported or stored in a place where the disposable containers 100 to 800 are to be used, through a packaging step of packaging them in a sterile environment.

For reference, the embodiments described above may be installed so as to be inclined or inverted upside down as necessary. That is, the covers 110 to 910 may be disposed on one side of the bases 130 to 930 or on the upper side.

This facilitates the installation of the support structures that support the unshown covers 110 to 910 and the bases 130 to 930, the convenience of connection of various lines (not shown) connected to the connection ports 131 to 931, (See reference numeral 150, 650, etc.), and the arrangement convenience of the stirring drive means 160 (refer to FIG.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the scope of the present invention is not limited to the disclosed exemplary embodiments, Other embodiments may easily be proposed by adding, changing, deleting, adding, or the like of a component, but this is also within the scope of the present invention.

10: Fusing means 11, 11a: Fusing head
12: corrugated part 13: fusion welding arm
20: Fusing means 21, 25: Fusing head
22: tensile projections 23, 27: welding arm
30: Fusing means 31: Fusing head
33: fusion welding arm
100: Disposable container of bioreactor
110: cover 111: first structural layer
112: coupling groove 113: second structural layer
114: coupling groove 115: third structure layer
116: coupling groove 130: base
131: connection port 132: fixing projection
134: Rounding parts 135, 136, 137:
135a, 136a, 137a: insertion grooves 135b, 136b, 137b:
150: stirrer 151: stirring body
153: stirring blade 155:
160: stirring driving means
200: Disposable container of bioreactor
210: cover 230: base
231: connection port 238: support rib
240: Temperature adjusting means 241: Heat medium circulation flow path
242, 243: heat medium circulation port 250: stirrer
260: stirring driving means 280: temperature adjusting means
281: Heat medium circulation flow path 282, 283: Heat medium circulation port
284: port groove 285: driving means insertion port
300: Disposable container of bioreactor
310: Cover 317: Cap coupling ball
330: Base 331: Port
350: stirrer 351: stirring main body
352: shaft coupling hole 353: stirring blade
360: stirring driving means 361: stirring shaft
370: Cap 371:
372: Inlet port 373: Fastening port
374:
400: Disposable container of bioreactor
410: cover 430: base
431: connection port 450: stirrer
460: stirring driving means 461: stirring shaft
470: Cap 471:
472: Inlet port 473: Fastening port
500: disposable container of bioreactor
510: Cover 530: Base
531: Connection port 532: Fixing projection
535: drive means insertion port 536: heat medium circulation flow path
537, 538: Heat medium circulation port
600: disposable container of bioreactor
610: cover 617:
630, 630a: base 631: connection port
632: Fixing projection 633:
638, 638a: latching jaw 639a:
650: stirrer 651: stirring body
652: shaft coupling hole 653: stirring blade
670: cap 672: inlet
673: Fastening port
700: Disposable container of bioreactor
710: Cover 730: Base
731: Connection port 732: Fixing projection
738: Retaining jaw 739: Tension groove
800: Disposable container of bioreactor
810: cover 820: connecting ring
838: fastening protrusion 829: fastening jaw
830: Base 831: Connection port
832: Fixing projection 838:
900: Disposable container of bioreactor
910: cover 920: reinforcing ring
928: fastening protrusion 930: base
931: Connection port 932: Fixing projection
938: fastening groove 939: engaging rib

Claims

A container-shaped cover made of at least one structural layer having flexibility and having a bottom open; And
And a base coupled to the open lower side of the cover and having a container shape with an opened upper surface and one or more connection ports protruding outward on the side surface,
The lower end of the cover is coupled to the upper end of the base
Disposable container of cell culture system.

The method according to claim 1,
A rounded portion is formed on an inner lower edge of the base
Disposable container of cell culture system.

The method according to claim 1,
Wherein the cover has a shape of a cylinder, an ellipse column, a prism, a cone or a pyramid, and the open top surface of the base has a shape corresponding to the shape of the open bottom surface of the cover
Disposable container of cell culture system.

The method according to claim 1,
The coupling of the cover and the base,
The inner peripheral surface of the lower end of the cover is fused to the outer peripheral surface of the upper end of the edge of the base or the outer peripheral surface of the lower end of the cover is fused to the inner peripheral surface of the upper end of the edge of the base
Disposable container of cell culture system.

5. The method of claim 4,
A number of latching jaws corresponding to the number of the structural layers along the outer periphery are sequentially formed on the upper end of the edge of the base toward the outward direction or the inward direction of the base
Disposable container of cell culture system.

6. The method of claim 5,
A coupling protrusion or an engaging groove is formed in a portion of the base which is in contact with one surface or the other surface of the end of the structure layer,
A coupling groove or a coupling protrusion corresponding to the coupling protrusion or the coupling groove is formed on one surface or the other surface of the end of the structure layer
Disposable container of cell culture system.

5. The method of claim 4,
A number of insertion grooves corresponding to the number of the structural layers is sequentially formed in the upper end portion of the base along the outer circumference toward the outward direction or the inward direction of the base,
The end of the structural layer is inserted into the insertion groove
Disposable container of cell culture system.

The method according to claim 1,
And a connecting ring interposed between the cover and the base
Disposable container of cell culture system.

9. The method of claim 8,
The outer circumferential surface of the connecting ring is fused to the outer circumferential surface of the lower end portion of the cover, or the inner circumferential surface of the connecting ring is fused to the outer circumferential surface of the lower end portion of the cover,
A fastening protrusion is formed on the lower side of the connection ring, and a fastening groove corresponding to the fastening protrusion is formed on the upper end of the base
Disposable container of cell culture system.

The method according to claim 1,
And a reinforcing ring having an inner circumferential surface welded to an outer circumferential surface of a lower end portion of the cover and a fastening protrusion formed on a lower side thereof,
A coupling groove corresponding to the coupling protrusion is formed at the upper end of the edge of the base,
And an engaging rib is protruded upward from an upper end of the base at a position closer to the center of the base than the engaging groove and the engaging rib is engaged with the engaging groove in the upper direction of the reinforcing ring when the engaging projection is engaged with the engaging groove Protruding
Disposable container of cell culture system.

5. The method of claim 4,
And at least one tensile groove is formed in a portion of the upper edge of the base which is welded to the lower end of the cover
Disposable container of cell culture system.

12. The method of claim 11,
Wherein the tensile grooves are elongated grooves extending in a direction parallel to the central axis of the base
Disposable container of cell culture system.

The method according to claim 1,
A heat medium circulation channel is formed in the base,
Wherein at least one pair of heat medium circulation ports communicating with the heat medium circulation flow path is formed on an outer circumferential surface of the base
Disposable container of cell culture system.

The method according to claim 1,
And a base temperature adjusting means coupled to the base to surround side and bottom surfaces of the base and having a heat medium circulating flow path formed therein and having at least one pair of heat medium circulating ports communicating with the heat medium circulating flow path formed on an outer circumferential surface thereof,
Disposable container of cell culture system.

The method according to claim 1,
And a cover temperature adjusting means which surrounds the outer circumferential surface of the cover and is supported by the base, a heat medium circulating flow path is formed in the inside thereof, and at least one pair of heat medium circulation ports communicating with the heat medium circulating flow path is formed on the outer circumferential surface thereof.
Disposable container of cell culture system.

The method according to claim 1,
The cover is formed with at least one ground wire,
When a vertical external force is applied to the cover, the portion of the cover where the contact wire is formed is folded and the length of the cover in the vertical direction is shortened
Disposable container of cell culture system.

17. The method of claim 16,
The folded portion of the cover is received within the base
Disposable container of cell culture system.

18. The method according to any one of claims 1 to 17,
A fixing protrusion or a fixing groove is formed in an intermediate portion of an inner bottom surface of the base
Disposable container of cell culture system.

19. The method of claim 18,
The fixing rib may be formed on the outer periphery of the fixing protrusion such that the mounting rib is protruded or the mounting groove is formed
Disposable container of cell culture system.

18. The method according to any one of claims 1 to 17,
And a cap coupled to the cap coupling hole, wherein the cap coupling hole is formed on at least a portion of the upper surface of the cover,
An edge portion of the cap is formed with an engaging portion that fuses with an edge portion of the cap engaging hole
Disposable container of cell culture system.

21. The method of claim 20,
A shaft hole is formed at an intermediate portion of the cap
Disposable container of cell culture system.