KR20160002093A - Cosmetic composition for skin diseases or disorders - Google Patents

Abstract

The present invention relates to a cosmetic composition for mitigating skin diseases or disorders, comprising a composite extract in which a Prunus mume extract and a natural beta-glucosidase-treated material extract which has antibacterial and anti-inflammatory activities against acne-causing bacteria and atopic dermatitis-causing bacteria are mixed. The natural beta-glucosidase-treated material extract comprises a Scutellaria baicalensis extract, a Coptis chinensis extract, a Morus alba bark extract, a Gardenia jasminoides extract, and a Platycodon glandiflorum extract. The composition comprises 0.1 to 5.0 wt% of the composite extract in which a Prunus mume extract and a beta-glucosidase-treated natural material extract which comprises a Scutellaria baicalensis extract, a Coptis chinensis extract, a Morus alba bark extract, a Gardenia jasminoides extract, and a Platycodon glandiflorum extract, are mixed based on the total weight of the composition.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition for skin diseases,

The present invention relates to a cosmetic composition for alleviating skin diseases, and more particularly, to a cosmetic composition for alleviating skin diseases, which comprises treating a golden extract, a Huanghui extract, a Ganoderma extract, a gardenia extract and a Gakgye extract with a beta-glucosidase enzyme, To a cosmetic composition for alleviating skin diseases in which an acne causative bacterium and an atopy-causing causative microorganism have been combined to enhance antibacterial and inflammation-inhibiting activity.

The cause of atopic dermatitis has not been known to date, and it is known that it has a genetic tendency, and it is now convinced that it is a kind of autoimmune disease. Unlike ordinary eczema or dermatitis, it represents an unusual symptom and course, which accounts for 70 to 80 cases of pediatric eczema. Atopic dermatitis is a disorder of skin barrier and abnormalities of immune function. Dysfunction of skin barrier facilitates skin penetration of pathogens as well as allergens and irritants. Immune dysfunction is caused by the reduction of antimicrobial peptides or barrier proteins by cytokines such as interleukin-4 (IL-4) and interleukin-13 (IL-13) and secondary infections with several microorganisms resulting in atopic dermatitis . In addition, most of the allergic atopic dermatitis is caused by immune mechanism associated with immunoglobulin E (IgE) antibody and mast cell, and delayed immune response due to T cell abnormality is involved rather than immediate type immune response to specific allergen . When the antigen is introduced, monocyte, macrophage, and B cells are appropriately treated with antigen-presenting cells (APC), and then the major histocompatibility complex (HMC) (IL-4), interleukin-5 (IL-5), interleukin-6 (IL-6) and the like, which are activated by T cells, . It is known that more than 90% of Staphylococcus aurreus is found in lesions of atopic dermatitis and deteriorates skin inflammation with toxins secreted by Staphylococcus aureus (Ahn, Gang Mo, Korea Society of Environmental Toxicology, Convention host, 2010.11, 48-49). In the treatment of atopic dermatitis, steroid external preparations, antihistamines, calcineurin inhibitors, antibiotics, and ultraviolet therapy are used. They are aimed at lowering the pruritus and alleviating the lesion by treating irritation. However, these treatment methods cause tolerance to the therapeutic drug and various side effects. Therefore, recently, there is a need for a treatment method with fewer side effects.

Acne is a skin disease caused by excessive activity of sebaceous glands due to the excessive activity of sebaceous glands, which can not escape smoothly from inside the pores, and can be combined with bacteria in the pores to cause inflammation. 70 to 80% It is concentrated in the age group, and mild acne is one of the characteristics of growing skin rather than skin disease. The causes of acne can be explained by the degree of development of acne symptoms due to the imbalance of hormone secretion, the increase of sebum secretion, the hyperkeratosis of the hair follicle, the proliferation of acne bacteria, and the inflammation reaction , And several mechanisms may also work together. One of the causes of acne is the proliferation of acne bacteria. Hair follicles blocked by sebum and dead skin cells create a good environment for bacteria to grow. Characteristically, acne is caused by a bacterial bacterium called propionibacterium acne, which is the main cause of acne as it grows on sebum. In normal people without acne, the bacterium normally exists on the skin and does not cause any problems, When the sebaceous glands accumulate in the hair follicles, they eat sebum and grow quickly. The various chemical components produced by these bacteria cause inflammation of the hair follicles and surrounding skin. Methods of treating acne include using antibiotics such as erythromycin or controlling sebum using estrogen, a female hormone, but have side effects. Although vitamin A derivatives, benzoyl peroxide, salicylic acid, and triclosan have been used in cosmetics for the treatment of acne, these substances have some antibacterial effects but have problems causing skin eruption, skin irritation, or side effects such as photosensitizers have.

Therefore, in order to prevent atopy disease and acne, it is necessary to develop a product which is safe for skin and human body while improving the suppression of bacterial growth and inflammation in lesions.

In addition, as a method for effectively improving the physiological activity of a natural product, a method of generating an aglycone by digesting sugar chains of glycosides commonly present in natural products with an enzyme has been studied. As a representative example thereof, Bifidobacterium lactis ), and a method for producing an isoflavone aglycosylated form of soybean using beta-glucosidase produced from the microorganism (Son Donghae, Korean Food Science Society, 2012).

The present invention has been made to solve the above problems of the prior art, and as a result, it has been found that a natural material extract, especially a gold extract, a Huanghui extract, a manganese berry extract, a gardenia extract, a Ginkgo extract, a beta-glucosidase- It is possible to provide a method for treating a medicinal enzyme which can effectively alleviate antimicrobial and inflammation-inhibiting activity caused by acne causative bacteria and atopy-causing causative bacteria, including a complex extract obtained by mixing the extract with plum extract. will be.

The present invention aims at providing a cosmetic composition for alleviating skin diseases, and more specifically, it relates to a cosmetic composition for alleviating skin diseases, which comprises at least one of a golden extract, an antioxidant extract, a Huanghui extract, a Ganoderma extract, a gardenia extract, It is intended to provide a cosmetic composition for alleviating skin diseases comprising a complex extract obtained by mixing an extract of a natural material with an enzyme-treated beta-glucosidase and a plum extract.

In order to achieve the above object, the present invention provides a cosmetic composition useful for alleviating atopic dermatitis and acne skin diseases, including a natural material extract obtained by treating with beta-glucosidase enzyme, wherein the beta-glucosidase enzyme treatment One natural medicinal herb extract is a complex extract obtained by mixing natural extracts of gold, chrysanthemum, mulberry, gardenia, and germicide with beta-glucosidase enzyme.

According to an embodiment of the present invention, the present invention provides a cosmetic composition for alleviating atopic dermatitis and acne skin disease, which comprises a complex extract of natural medicinal herb extract treated with the beta-glucosidase enzyme as an active ingredient.

According to an embodiment of the present invention, the present invention provides a cosmetic composition for alleviating atopic dermatitis and acne skin disease, which comprises an extract of natural medicinal herbs treated with the beta-glucosidase enzyme as an active ingredient.

The cosmetic composition for alleviating skin diseases according to the present invention can be effectively used for alleviating atopic dermatitis and allergic dermatitis by antimicrobial, inflammation inhibiting activity and antioxidative effect against acne causative bacteria and atopy causative bacteria.

Accordingly, the cosmetic composition for relieving skin diseases according to the present invention can be used as a functional cosmetic composition and the like as an active ingredient of a natural medicinal herb extract prepared by the beta-glucosidase enzyme treatment, and can be used for the treatment of atopic dermatitis and allergic dermatitis, In addition to the improvement and / or inhibition and / or inhibition and / or therapeutic effect, the preventive effect such as atopic dermatitis can be obtained by continuous use even before the onset.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing the effect of inhibiting inflammatory-related gene expression of interleukin 1 and the like in one embodiment of the cosmetic composition of the present invention compared with the control group and the comparative example.
FIG. 2 is a graph showing the effect of inhibiting gene expression of interleukin 2, which is an atopic related index of one embodiment of the cosmetic composition of the present invention, with the control group and the comparative examples.
Fig. 3 is a graph showing the antioxidative effect of DPPH in one embodiment of the cosmetic composition of the present invention compared with the control group and the comparative examples. Fig.
4 is a graph showing the inhibitory rate of NO production of one embodiment of the cosmetic composition of the present invention compared with the control group and the comparative example.

Hereinafter, specific embodiments of the present invention will be described in detail with reference to the accompanying drawings.

The cosmetic composition for alleviating skin diseases according to the present invention is characterized in that a mixture of a gold extract, a Huanghui extract, a Huanghui extract, a gardenia extract, and a Gakgye extract is treated with a beta-glucosidase enzyme-treated natural herbal extract and a plum extract And 0.1 to 5.0% by weight of the combined complex extract.

In the present invention, the "alleviation" should be understood as including the prevention and / or amelioration and / or inhibition and / or inhibition and / or treatment of skin diseases.

The golden extract, Huangliao extract, manganese berry extract, ginger extract, gakgye extract and plum extract, which are natural materials extracts used in the production of the natural medicinal herb extract treated with the beta-glucosidase enzyme used in the present invention, Phosphorus extraction method may be used, and a hot water extraction method or ethanol or a mixture obtained by mixing water and ethanol may be used.

The beta used in the enzyme treatment-glucosidase is Aspergillus you this (Aspergillus niger .

The golden ( Scutellaria baicalensis ) is a perennial herbaceous perennial plant of the dicotyledonous plant, monocotyledonous plant, and is distributed mainly in Korea, China, and Siberia. It is said to be a decaying grass, a scavenger, and a fragrance. In the oriental medicine, the roots are used as fever, diuretic, branch, ejaculatory and anti-inflammatory drugs. It is known that baicalin and wogonoside, which are flavonoid-based glycosides, and baicalein and wogonin, which are non-glycosides, are present as major active ingredients of gold It is known that the non-glycoside form is more bioactive than the glycoside. In recent years, studies on the efficacy of the active ingredients such as hepatocyte protection, inhibition of liver cirrhosis, inhibition of stomach damage due to alcohol, suppression of gastric acid, suppression of Helicobacter pylori, and pain relief such as joints are underway.

The Coptis chinensis is the root of the mulberry tree. Yellow roots are said to be one after another. Nowadays, larvae of buttercups are mainly communicated, and berberine is known to be similar. Anti-inflammatory, sedative, nervous anxiety, hyperemia and inflammation. It is also used for bad digestion and gastritis due to bitter taste. In addition, eye inflammation is washed in water with antler.

Morus alba , the deciduous tree belonging to Moraceae , and the root of the same relative plant are dried, and in the clinical herbal medicine (Yeonglimsa 2000) It is said that there is the efficacy of Isuzu.

The above-mentioned gardenia is an evergreen shrub belonging to the family Rubiaceae ( Gardenia jasminoides ) and mature fruit of the relative plants were dried. In the clinical herbal medicine (Yeonglimsa, 2000), it was found that the effects of sulphate extermination, It is said to have efficacy.

The ginseng extract is an extract of Gakgyeong, or bellflower. The bellflower is a native material throughout Korea, and it is a common material to be recognized as one of the three tricolor herbs that are widely used as bellflower seeds, bellflower herbs, and so on. Old bellflower is said to be better than wild ginseng. In fact, the excellent alkaline food, bellflower, dietary fiber as well as minerals such as calcium, iron, potassium, vitamin B1, B2, vitamin C and tyrosine, tryptophan and rich beneficial amino acids. Because of these nutrients, the Dongbu feelings are said to have 278 records of the effectiveness of the bellflower. Bellflower is a perennial plant belonging to lanterns. It is also called Gakgyeong, Bungdo, Hwacheon, Gyeongcho, Gogyeong, Lee. It is mainly used as a medicine for roots. It is picked in autumn, peeled and dried in the sun, then peeled off the faucet. Platycodin, a kind of saponin, and platycodigenin are contained in roots. Balloons are commonly known to be used for coughs and phlegm. Especially, in bellflower, saponin having a brittle taste, which is known as a major component of ginseng, is contained about 2 g per 100 g. In the case of triterpenoid saponin, it shows efficacy against respiratory diseases. It is known to increase the secretion of mucus from the respiratory mucosa, and to reduce sputum. Bellflower juice, which is a blend of bellflower and pear, is widely used as a preventative for winter colds. The main ingredient of Yonggak Mountain, which is famous for cough and phlegm, is bellflower. Especially in oriental medicine, it is known that it is effective for drainage, genomes, tonsillitis, Choi yu, Jinhae, purulent boils, expectorant of asthma and pulmonary tuberculosis, and pleurisy. Bellflower has the ability to lower blood pressure and blood sugar. It is said that it is beneficial for people with high blood pressure and diabetes who are overweight. Platycodon is known to help people with hyperlipidemia because it enhances secretion of steroids and galic acid in the body and leads to a decrease in body cholesterol. In recent years, tumor suppressing substances have been isolated from platycodon, and antimutagenic effects have also been confirmed. It has also been found that when a substance extracted from ethyl acetate of bellflower is reacted with liver cancer cells, it has a strong antioxidative effect and protects cells against cancer due to oxidative stress.

The plum is plum ( Prunus mume Sieb . meat Zucc . ) Is a deciduous tree belonging to the Rosaceae and its origin is China. Plums are green with a ball shaped core. This fruit ripens yellow in July, has a diameter of 2 to 3, is dense and has a sour taste. This fruit is called plum. Plum has been used as a dietary supplement and health supplement for over 3,000 years. It is believed to have been used as a medicinal plant in Korea since it was handed down as a garden water in the Three Kingdoms period in Korea. Fruit of the fruit is about 80%, of which about 85% is composed of water and about 10% of sugar. It is rich in minerals, vitamins, organic acids (citric acid, malic acid, succinic acid, tartaric acid), and contains minerals such as calcium, phosphorus and potassium and carotene. Among them, citric acid promotes the metabolism of saccharides and relieves fatigue, and the organic acid acts to stimulate the action of the stomach and increase the appetite. Alkaline food is good for fatigue recovery, especially detoxification, it helps to treat stomach and food poisoning, sour taste secretes gastric juice and normalizes digestive organs to eliminate digestive and gastrointestinal disorders. Good for constipation and skin beauty, high acidity and has a powerful sterilizing action.

The cosmetic composition for alleviating skin diseases according to the present invention is characterized in that a natural medicinal herb extract obtained by treating a mixture of a gold extract, a Huanghui extract, a Huanghui extract, a gardenia extract, and a ginseng extract with a beta-glucosidase enzyme, And 0.1 to 5.0% by weight of the combined complex extract. Within this range, there is an advantage that the agent for improving skin diseases and the skin inflammation-suppressing effect are particularly excellent.

The cosmetic composition for alleviating skin diseases is preferably used in an amount within a range of 3 to 8, preferably in an amount of 4 to 5, which is slightly acidic.

The formulation of the cosmetic composition for alleviating skin diseases according to the present invention is not particularly limited and may be appropriately selected according to the purpose. For example, the cosmetic composition of the present invention can be manufactured in formulations such as ointment for skin, softening longevity, nutrition lotion, nutritional cream, massage cream, essence, pack, emulsion, oil gel and the like, The present invention is not intended to be limited thereto.

The ointment for external use on skin may be prepared so as to contain 50 to 97% by weight of vaseline and 0.1 to 5% by weight of polyoxyethylene oleyl-ether phosphate in addition to the natural drug extract obtained by treating with the beta-glucosidase enzyme, 1 to 10% by weight of polyhydric alcohols (propylene glycol, glycerin, etc.) and 0.05 to 2% by weight of a surfactant (polyethylene oleyl ether, polyoxyethylene hydrogenated castor oil, etc.) in addition to the natural drug extract obtained by treating with the above-mentioned beta-glucosidase enzyme %. ≪ / RTI >

The nutritional lotion and nutritional cream may contain 5 to 20% by weight of oils (squalane, vaseline, octyldodecanol, etc.) and wax components (such as cetanol, stearyl alcohol, Beeswax, etc.), and the essence is prepared by including 5 to 30% by weight of polyhydric alcohols such as glycerin and propylene glycol in addition to the natural drug extract obtained by the above-mentioned beta-glucosidase enzyme treatment .

The massage cream is prepared by adding 30 to 70% by weight of an oil such as liquid paraffin, petrolatum, isononylisononanoate and the like in addition to the natural medicinal plant extract treated with the beta-glucosidase enzyme treatment. The pack contains the beta-glucoside In addition to the natural medicinal plant extract treated with the beta-glucosidase enzyme, peel off packs containing 5 to 20% by weight of polyvinyl alcohol or common emulsifying cosmetics, , Zinc oxide, titanium dioxide and the like in an amount of 5 to 30% by weight. In addition, the cosmetic composition for alleviating skin diseases of the present invention may contain, in addition to the above-described ingredients, the ingredients, water, surfactant, moisturizer, lower alcohol, thickener, chelating agent, coloring agent, preservative, And the like can be appropriately used in combination as needed.

Hereinafter, preferred embodiments and comparative examples of the present invention will be described.

The following examples are intended to illustrate the invention and should not be construed as limiting the scope of the invention.

Example 1 shown in the following Table 1 is a cosmetic composition for alleviating skin diseases, including a golden extract, a Huangyan extract, a Manganese bark extract, a gardenia extract, a Ginkgo extract and a plum extract according to the present invention.

Example  One

100 g of the pulverized pulverized product was put into 3 liters of a mixed solvent of 70% by weight of ethanol and 30% by weight of water, and the mixture was added to a reflux extractor equipped with a cooling condenser It was boiled and extracted for 3 hours. After filtration through a 100-mesh filter cloth, the residue was extracted one more time by the same method. Each of the extracts was combined and filtered through a filter paper of Whatman No. 2 at room temperature to remove insoluble matter. The extract was concentrated under reduced pressure to a solid content of 70% at 60 ° C in a distillation apparatus equipped with a cooling condenser to obtain a first extract. As a plum extract, a commercially available 70% solids concentrate was purchased and used. Enzyme treatment of the extract is Aspergillus you this (Aspergillus niger , A. niger ) -derived beta-glucosidase (Sigma, USA) was used. Beta-glucosidase was dissolved in an acetic acid buffer solution (pH 4.6) and mixed at a ratio of 10 units / ml (1.33 mg / ml) to a natural material composite and reacted at 45 ° C for 24 hours. The composition of the above-mentioned natural material extracts, that is, the combination of the gold extract, Huangliao extract, mulberry extract, ginger extract, Ginkgo extract, and the plum concentrate as a plum extract of the beta-glucosidase enzyme-treated natural material extract, Respectively.

sample Comparative Example 1 Example 1 First extract 2g - Plum concentrate 8g - Enzyme treated natural materials complex
(First extract: plum concentrate = 2: 8) 10g

<Experimental Example 1> Test of antibacterial activity against atopic dermatitis and acne-causing bacteria

1 g of each of the compositions of Comparative Example 1 and Example 1 was diluted and suspended in 9 g of sterilized phosphate buffer, and the experiment was conducted as follows.

The diluted suspension was serially diluted 2-fold with BHI (Brain heart infusion) medium and injected into a 48-well plate in an amount of 1 ml each. In agar medium, Staphylococcus aurreus , ATCC 25923) was suspended in 20 mL of BHI medium, and then added to the diluted 48-well plate. After incubation at 37 ° C for 24 hours, the antibacterial activity was measured by MIC (minimal inhibition concentration) Respectively. The results are shown in Table 2 below. Propionibacterium acne , an anaerobic microorganism, was diluted and suspended in 9 g of sterilized phosphate buffer solution as in Experimental Example 1, and 1 g of the mixed composition of Comparative Examples and Examples was diluted and suspended in the following manner. The MIC method was performed in the same manner as in Experimental Example 1. However, since the anaerobic microorganisms do not grow in the presence of oxygen, they were cultured in an anaerobic incubator (Sheldon Manufactoring Bac 2-2E) fed with 5% hydrogen (H ₂ ) and 95% nitrogen (N ₂ ) The results are shown in Table 3 below.

Strain (%) Comparative Example 1 Example 1 Staphylococcus aureus
ATCC 25923 2.5 1.25 Propionibacterium acne
KCTC 3314 10 5

As a result, according to the present invention, when the golden extract, Huangryu extract, mulberry extract, germanium extract, and Gakgye extract were enzymatically treated and mixed with mulch extract to make a complex extract, Staphylococcus aurreus (ATCC 25923) and Propionibacterium acne (KCTC 3314), which is an acne-causing microorganism, are superior to the antimicrobial activity against atopic dermatitis and acne.

Example  2

Using the complex extract obtained by mixing beta-glucosidase enzyme-treated natural material extract with plum extract, which is a form of the gold extract, Huangliao extract, manganese extract, germanium extract and Gilbang extract mixture of Example 1, Skin external ointment was prepared.

Example 2 Comparative Example 2 The natural material extract of Example 1 0.5 - Diethyl sebacate 8 8 Nonsense 5 5 Polyoxyethylene oleyl ether phosphate 6 6 Sodium benzoate Suitable amount Suitable amount vaseline Up to 100 Up to 100

Example  3

Using the complex extract obtained by mixing beta-glucosidase enzyme-treated natural material extract with plum extract, which is a form of the gold extract, Huangliao extract, manganese extract, ginger extract, and Gilbang extract mixture of Example 1 described above, Cream.

Example 3 Comparative Example 3 The natural material extract of Example 1 0.5 - Stearic acid 15.0 15.0 ethanol 1.0 1.0 Potassium hydroxide 0.7 0.7 glycerin 5.0 5.0 Propylene glycol 3.0 3.0 antiseptic Suitable amount Suitable amount incense Suitable amount Suitable amount Purified water Up to 100 Up to 100

Example  4

Using the complex extract obtained by mixing Beta-glucosidase enzyme-treated natural material extract, which is a form of the gold extract, Huangliao extract, Mulberry bark extract, ginger extract and Gil Gyeongguk extract mixture of Example 1, with plum extract, The softening longevity was produced.

Example 4 Comparative Example 4 The natural material extract of Example 1 0.3 - ethanol 10.0 10.0 Polyoxyethylene sorbitan polylauric acid 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 glycerin 5.0 5.0 1,3-butylene glycol 6.0 6.0 incense Suitable amount Suitable amount Pigment Suitable amount Suitable amount Purified water Up to 100 Up to 100

Example  5

Using the complex extract obtained by mixing beta-glucosidase enzyme-treated natural material extract with plum extract in the form of the gold extract, Huangliao extract, manganese extract, ginger extract, and Gilligan extract mixture of Example 1 described above, Nutritional lotion was prepared.

Example 5 Comparative Example 5 The natural material extract of Example 1 0.1 - Vaseline 2.0 2.0 Sesquioleic acid sorbitan 0.8 0.8 Polyoxyethylene oleylethyl 1.2 1.2 Methyl paraoxybenzoate Suitable amount Suitable amount Propylene glycol 5.0 5.0 ethanol 3.2 3.2 Carboxyvinyl polymer 18.0 18.0 Potassium hydroxide 0.4 0.4 incense Suitable amount Suitable amount Pigment Suitable amount Suitable amount Purified water Up to 100 Up to 100

Example  6

Using the complex extract obtained by mixing beta-glucosidase enzyme-treated natural material extract with plum extract, which is a form of the gold extract, Huangliao extract, mulberry extract, germanium extract and Gilbang extract mixture of Example 1, Pack.

Example 6 Comparative Example 6 The natural material extract of Example 1 0.4 - glycerin 5.0 5.0 Propylene glycol 4.0 4.0 Polyvinyl alcohol 15.0 15.0 ethanol 8.0 8.0 Polyoxyethylene oleylethyl 1.0 1.0 Methyl paraoxybenzoate 0.2 0.2 incense Suitable amount Suitable amount Pigment Suitable amount Suitable amount Purified water Up to 100 Up to 100

Example  7

Using the complex extract obtained by mixing Beta-glucosidase enzyme-treated natural material extract with plum extract, which is a form of the gold extract, Huangliao extract, mulberry extract, germanium extract, and Gilligan extract mixture of Example 1, Essence was prepared.

Example 7 Comparative Example 7 The natural material extract of Example 1 5.0 - glycerin 10.0 10.0 Sodium hyruronate 0.05 0.05 Propylene glycol 10.0 10.0 ethanol 5.0 5.0 Polyoxyethylene hardened castor oil 1.0 1.0 Methyl paraoxybenzoate 0.1 0.1 incense Suitable amount Suitable amount Pigment Suitable amount Suitable amount Purified water Top 100 Top 100

Experimental Example 2 Evaluation of inflammation inhibition

Macrophage-derived cells (RAW 264.7 cell line) were cultured in Raw264.7 cells with Tumor necrosis factor-alpha, TNF- alpha, cyclooxygenase-2, COX-2, interleukin IL- Beta (IL-1) and interleukin IL-6 (IL-6) gene expression were evaluated as follows.

First, RAW 264.7 cells were seeded on a 60 mm dish at 4.0 × 10 ⁶ cells and cultured for 18 hours. Tumor necrosis factor-alpha, TNF-alpha, cyclooxygenase-2 and COX-2 were pretreated at a concentration of 5 mg / ml in Comparative Example 1 and Example 1, IL-2 (IL-2), interleukin (IL-1) and IL-6 were treated with IL-1 and IL- IL-5 (IL-5) and interleukin IL-6 (IL-6) were treated with 25 nM PMA (phorbol 12-myristate) and 125 nM A23187 (Calcium Ionophore) for 6 hours. The cells were harvested, centrifuged at 4,000 rpm for 5 minutes at 2,000 rpm, and total RNA (total RNA) was isolated using Easy Blue Reagent (iNtRON Biotechnology). CDNA was synthesized using the QuantiTect Reverse Transcription kit (Qiagen, Hilden, Germany) as a separate RNA. To perform PCR on the synthesized cDNA, 1 g of cDNA was added with a primer primer) was mixed with 1 L and 10 buffer solutions (10 mM Tris-HCl, pH 8.3, 50 mM KCl, 0.1% Triton X-100), 250 M dNTP and 1 U Taq polymerase) The primers were amplified 24 times at a temperature of 50 ° C / 0.5 min, 58 ° C / 1 min and 72 ° C / 1 min, respectively.

gene sequence IL-1 sense 5'-atgaagttcctctctgcaag -3 ' Antisense 5'-cactaggtttgccgagtaga -3 ' IL-6 sense 5'-atgaagttcctctctgcaag -3 ' Antisense 5'-cactaggtttgccgagtaga -3 ' TNF- sense 5'-cggactccgcaaagtctaag -3 ' Antisense 5'-acggcatggatctcaaagac -3 ' COX-2 sense 5'-cggactccgcaaagtctaag -3 ' Antisense 5'-acggcatggatctcaaagac -3 ' IL-2 sense 5'-tgctccttgtcaacagcg-3 ' Antisense 5'-tcatcatcgaattggcactc-3 ' IL-4 sense 5'-gaagaccttggcactgctttc-3 ' Antisense 5'-atctttggctgcaacaaacc-3 ' IL-5 sense Antisense

As shown in FIG. 1 and FIG. 2, Example 1 shows the expression of IL-1 and IL-1 in IL-1 and IL-2 in inflammatory cytokines relative to control and Comparative Example 1 It is considered that it would be effective for atopic inflammation and acne inflammation. In addition, the composition of Example 1 according to the present invention effectively inhibits interleukin-2 (IL-2), interleukin-5 (IL-5) and interleukin-6 (IL-6), which are related to the immune response of atopic dermatitis .

As seen from the above results, it was found that the present invention is sufficient for use as a cosmetic product in view of the excellent effect of inflammation and anti-atopy in Example 1.

&Lt; Experimental Example 3 >

Nitric oxide (NO) production was evaluated using a GRIESS reagent using Raw 264.7 cell line (ATCC CRL-2278). Raw 264.7 cells previously cultured in DMEM supplemented with 10% fetal bovine serum (FBS) were inoculated into a 96-well plate at a concentration of 1 × 10 ⁵ cells / And cultured in a carbon dioxide incubator (CO ₂ incubator) for 24 hours. After the medium was removed, the cell culture medium was replaced with DMEM without serum, and the diluted sample was added to the medium for 15 minutes. Lipopolysaccharide was added at a concentration of 0.5 占 퐂 / ml for 16 hours, 100 占 퐇 of the supernatant was transferred to a 96-well plate, 100 占 퐇 of a green reagent was added thereto, and the mixture was reacted at room temperature for 15 minutes. And the absorbance at 540 nm was measured with an ELISA reader.

The antioxidant activity was evaluated by DPPH (1,1-diphenyl-2-picryl-hydrazyl) method. 1 ml of the 0.1 mM DPPH solution was added to 1 ml of the extract of natural materials diluted to 0.01% and 0.001% with anhydrous ethanol and reacted at 37 ° C for 30 minutes. The absorbance of the reaction product was measured at 516 nm using a spectrophotometer Respectively. Butylated hydroxytoluene (BHT) was used as a positive control and anhydrous ethanol was used as a negative control.

As shown in FIG. 3 and FIG. 4, it was confirmed that the composition containing the natural material extract according to the present invention is superior in antioxidant activity and inhibition of nitric oxide production. It is known that inhibition of the generation of nitric oxide and antioxidant activity are important for inhibiting and treating inflammation. These results support the anti-inflammatory effect.

The present invention can be used in the industry for producing cosmetic compositions.

While the invention has been shown and described with reference to certain exemplary embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims.

(Without reference numerals)

Claims (7)

A mixture of a gold extract, a Huanghui extract, a Ganoderma lucidum extract, a gardenia extract, and a Gakyung extract as a natural material extract on the basis of the total weight of the composition is mixed with a combination extract of Beta-glucosidase enzyme-treated natural material and a plum extract, By weight based on the total weight of the cosmetic composition. The method according to claim 1,
The above-mentioned natural substance extract of Beta-glucosidase enzyme is a mixture of a mixture of a golden extract, a Rhodiola extract, a Ganoderma lucidum extract, a gardenia extract, a Ginkgo extract and a mixture obtained by treating with Beta-glucosidase and the above plum extract in a weight ratio of 1: 3 to 5 By weight based on the total weight of the cosmetic composition. The method according to claim 1,
Wherein the cosmetic composition has a pH within a range of 4 to 8. The method of claim 3,
Wherein the cosmetic composition for relieving skin diseases is weakly acidic. The method according to claim 1,
A cosmetic composition for alleviating skin diseases, wherein the cosmetic composition for relieving skin diseases is a paste. The method according to claim 1,
Wherein the skin disease cosmetic composition is in a liquid phase. The method according to claim 1,
Wherein the skin disease cosmetic composition is manufactured by a formulation such as ointment for skin, softening longevity, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion, oil gel and the like.

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