KR20120055159A - A composition for reducing sleep induction time and prolonging sleeping time containing chrysanthemum indicum extract, and preparing method for the same - Google Patents

Abstract

PURPOSE: A composition for preventing and treating insomnia is provided to prevent and treat insomnia. CONSTITUTION: A composition for preventing and treating insomnia contains 50mg/kg or more of Chrysanthemum indicum L. extract. The composition can be applied to foods such as biscket, candy, tea, and supplementary health food. The extract is prepared by extracting Chrysanthemum indicum L. with 40-80 wt% of low carbon number alcohol, filtering, concentrating, and drying. The dose of the composition is 10-5,000 mg/day.

Description

A composition for reducing sleep induction time and prolonging sleeping time containing Chrysanthemum indicum extract, and preparing method for the same}

The present invention relates to a composition for the prevention and improvement of insomnia symptoms and a method for preparing the same, wherein the composition is prepared by using the extract as an active ingredient to shorten the sleep induction time and to prolong the sleep time. Cl ^- confirmed that promote the influx of), and reduce the surface inducing the object time when applying them, and to provide a sleep time for the prevention and improvement on that can be useful to extend insomnia a composition and a method of manufacturing the same will be.

In modern society, many people suffer from sleep disorders due to stress, anxiety, and anxiety. However, when the period is short and weak, medication is not required. According to statistics, about 15% of adults are in need of drug treatment due to insomnia caused by anxiety symptoms. Increasing age increases the insomnia patients.

There are various causes of insomnia, such as stress, excessive anxiety, tension, and fear.Therapeutic drugs include benzodiazepines and serotonin agonists. In addition, it is necessary to develop drugs that can treat anxiety and insomnia with fewer side effects.

On the other hand, the fact that the gakguk extract to be proposed in the present invention is effective in the prevention and improvement of insomnia symptoms are not described or implied in any literature.

Accordingly, the inventors of the present invention have been researched to solve the above problems, and found that the gamguk extract can improve the effect of shortening the sleep induction time and extending the sleep time, thereby completing the present invention.

Therefore, an object of the present invention is to provide a composition for preventing and improving insomnia and a method for manufacturing the same, which can be effectively used to shorten the sleep induction time and extend the sleep time.

As an example for achieving the above object, the composition for the prevention and improvement of insomnia symptoms of the present invention is characterized in that it comprises a gamguk extract as an active ingredient.

The persimmon extract is characterized in that it comprises at least 50 mg / kg in an effective amount in the composition.

As another example of the present invention for solving the above problems, a functional food comprising a composition for the prevention and improvement of the insomnia.

As another example of the present invention for solving the above problems, the method for producing a composition for the prevention and improvement of insomnia symptoms, extract, filtration, concentration and the like in the aqueous solution containing 40 to 80% by weight of lower alcohol having 1 to 4 carbon atoms It is characterized in that it comprises a process of obtaining the extract by drying the soup.

Hereinafter, the composition for the prevention and improvement of insomnia symptoms of the present invention and the preparation method thereof will be described in detail for each component and manufacturing steps.

First, insomnia or insomnia used in the present specification and claims mean a sense that is not enough to wake up refreshed, and is reported to affect about 15% of all adults in Korea annually. The term is widely used as meaning any and all types of sleep loss. In addition, the improvement may reduce, prevent (eg, prevent), reverse (eg, alleviate) or reduce insomnia and other symptoms associated with sleep disorders and other conditions that interfere with normal sleep, Means to improve, control or manage well.

Gamguk ( Chrysanthemum indicum L.) is a perennial herb belonging to the Asteraceae family, also known as Huangguk (黃菊). It grows mainly in the mountains, and in October, the flowers are dried and drunk, or young leaves are used as herbs. Gamguk is used to lighten the body, not to grow old, to longevity, to strengthen the musculature, to see the bone marrow, to brighten the eyes, to treat punghyun headache, and to drink and not wake up.

The gamguk extract can be obtained by extracting, filtering, concentrating and drying the gamguk in an aqueous solution containing 40 to 80% by weight of lower alcohol having 1 to 4 carbon atoms. It is characterized by comprising the process of obtaining.

More specifically, after mixing and immersing the aqueous solution containing 40-80% by weight of lower alcohol with 1 to 4 carbon atoms in a ratio of 1: 5 to 20% by weight, ultrasonic extraction, reflux extraction, heating extraction (40 to 80 ℃), etc. Extraction is carried out in various ways, wherein the extraction step is repeated 2 to 5 times in terms of yield of the extraction of the effective ingredient in the country. The resultant extracted by the aqueous alcohol solution is sterilized and filtered. High-pressure sterilization in consideration of the maintenance and sterilization of nutrients can minimize the denaturation of other nutrients, and can achieve sterilization effects. The filtrate can be concentrated and dried in a variety of ways and used, and tends to prefer reduced pressure concentration or lyophilization.

Filtration, sterilization, concentration and drying in the preparation of the gamgukchi extract can be applied by appropriately adopted methods commonly accepted in the art and the present invention is not limited by their selection.

Such a gamguk extract is preferred in terms of economics and expression of the improvement and prevention of insomnia symptoms when in an effective amount of 50 mg / kg or more, specifically 50 to 500 mg / kg included in the composition. If the content of persimmon extract is high, the flavor of the persimmon soup may be uncomfortable.

Improvement of insomnia symptoms can be measured both quantitatively and qualitatively. For example, in qualitative cases, improving insomnia may result in faster sleep at bedtime, reduced frequency of waking in the night, waking up at the right time, and a good night's sleep that allows the individual to feel refreshed the next day. It can be measured by any number of factors, including but not limited to taking.

Factors that can be used to measure the quantitative improvement of insomnia symptoms include sleep polymorphism tests that indicate increased sleep efficiency, more time to sleep at deeper levels of sleep, such as steps 2 to 4 opposite to step 1, It includes, but is not limited to, a reduction in the number of periodic cognitive movements.

The present inventors confirmed that the extract of Gingerbread has an effect of shortening the sleep induction time or extending the sleep time. Also within the chloride ion (Cl ^-) nerve to investigate the mechanism of action thereof, the cell ^was confirmed that the amount of chloride ions increase results of measuring the amount (Cl). In addition, the expression of glutamic acid decarboxylase (GAD) was measured, and it was confirmed that the amount of GAD protein was increased.

The composition for the prevention and improvement of insomnia according to the present invention may include a pharmaceutically acceptable carrier in addition to the gamguk extract, such carriers, excipients, binders, lubricants, disintegrants, coatings, emulsifiers, suspensions, And at least one carrier selected from the group consisting of solvents, stabilizers, absorption aids, water for injection, isotonic agents and the like.

The composition for the prevention and improvement of insomnia according to the present invention may be formulated in oral or injectable preparations, preferably in oral preparations. Specific examples of such oral preparations include granules, tablets, capsules, and liquids.

The frequency and dosage of the composition for preventing and improving insomnia according to the present invention may vary depending on the age, sex, and weight of the patient, but based on the adult male (70 kg body weight), In view of the above, it is preferred that it is 10 to 5,000 mg / day, more preferably 50 to 2,000 mg / day.

The gamgukg extract forming the composition for the prevention and improvement of insomnia symptoms of the present invention has been proven to be toxic and stable as a raw material has been eaten as a food or medicine for a long time, and is used for the prevention or treatment of existing insomnia symptoms Unlike the old composition, there is another advantage that does not cause poisoning or dependency due to toxicity.

The composition for preventing and improving insomnia symptoms of the present invention can be prepared and ingested as an oral preparation for preventing and improving insomnia symptoms, and can be prepared and consumed in various forms of functional foods in a range that does not impair the intrinsic taste of food. . Non-limiting examples of such functional foods include biscuits, candy, tea, dietary supplements and the like.

In addition, the composition for the prevention and improvement of insomnia according to the present invention can be applied to the warm-blooded animals such as rats, dogs, rabbits, cats, chickens, cattle, pigs as well as humans can be applied for the improvement of insomnia symptoms.

As described above, according to the composition for preventing and improving insomnia symptoms of the present invention, it can be seen that the human body is safe and less toxic and has a tendency to clearly promote the influx of chloride ions (Cl ⁻ ) into neurons.

Accordingly, the composition for the prevention and improvement of insomnia symptoms of the present invention can shorten the sleep induction time and prolong the sleep time can be expected to the effect of preventing and improving insomnia symptoms.

1 is a graph showing the effect of the extract of gamguk on pentobarbital induced sleep (* P <0.05, *** P <0.005) [(A) shows the effect on sleep time, (B) Effect on induction time].
Figure 2 is a graph showing the effect of the extract (ECF) on chloride influx (PENT: pentobarbital. * P <0.05, *** P <0.005, compared to the control).
Figure 3 is a graph showing the effect of the extract of gamguk on glutamic acid decarboxylase (GAD) (PENT: pentobarbital. * P <0.05, compared to the control)

Hereinafter, the present invention will be described in detail with reference to Examples and the like, but the present invention is not limited to the following Examples and the like.

Example  One. Country  Preparation of Extract

After purchasing and drying the persimmon soup, the dried persimmon soup and 70% ethanol (40-80 ° C.) were mixed at a ratio of 1: 4 by weight, and extracted twice by heating. .

Example  2. Pentobarbital  Measurement of Sleep Induction Time and Sleep Time in Mice Induced

Mice (males with an average body weight of 20 g, purchased from Korean experimental animals) were starved for 24 hours prior to pentobarbital administration, followed by intraperitoneal administration of pentobarbital 42 mg / kg. Sleep was the point at which clove reflections disappeared, animals not sleeping within 15 minutes were excluded from the test, and waking time was defined as the time for the animals to return to cloves.

When the content of the gakguk extract obtained in Example 1 was administered in different amounts, the effects on pentobarbital-induced sleep induction time and sleep time according to the amount of use were shown in FIG. 1.

As shown in FIG. 1, the administration time of 20, 50 and 100 mg / kg of the extract of Gamguk was significantly increased compared to the control group when 50 and 100 mg / kg was administered (A), and the sleep induction time was the control group. Compared to the control group when administered 50 mg / kg compared to the significantly increased (B).

In addition, the effect of the gamguk extract on the sleep of the pentobarbital minimum sleeping dose (28 mg / kg) is shown in Table 1 below.

group Dose (mg / kg) Number of Animals Sleeping / Number of Experiment Animals Sleep time (minutes) Control group 0 6/15    23.3 ± 1.8 Muscimol ^{1 )} 0.2 13/14 ^** 41.2 ± 4.2 ^*** ECF ^{2 )} 20 7/14    28.0 ± 4.0 50 7/14    29.6 ± 3.2 100 11/13 ^* 35.9 ± 3.6 ^{* *} P <0.05, ^** P <0.01, ^*** P <0.005 vs. Control
1) Muscimol: reference drug
2) ECF: Gammon Extract

As shown in Table 1 above, as a result of measuring the sleep increase effect induced by the minimum concentration of pentobarbital, the gamguk extract was administered in combination with the minimum dose of pentobarbital to induce sleep. Increased. This shows that the extract of Gam-guk shows an enhancement effect when used with sleeping pills.

On the other hand, pentobarbital 28 mg / kg of 6 animals sleep in 15, and when combined with the gamguk extract, the number of sleeping animals increases dose-dependently, 11 out of 13 at 100 mg / kg of gamguk extract Sleep.

Example  3. Neuronal cell culture and Intracellular  Chloride ions ( Cl ^- )

The cerebellum was removed from the brain of the fetus from 8 days of pregnancy (SD rats purchased from Korean experimental animals, with an average body weight of 200 g), the cerebrospinal fluid was removed, and the neurons were separated. Incubate for 18 to 24 hours in a% carbon dioxide gas incubator, and then add cytosine arabinofuranoside to prevent growth of non-neuronal cells and incubate for 6 to 12 days to measure chloride ions (Cl ⁻ ) It was.

Chloride ion (Cl ^-) of the measurement is this (MQAE) one was treated with a solution mixed with a chloride ion buffer was allowed to stand overnight incubation for 5 minutes, a chloride ion as a fluorescent measuring instrument (Cl ^-) in emkyu the neurons was measured The results are shown in FIG.

Also, in a chloride ion (Cl ^-) cells compared to control when, hayeoteul 4μg / ml when processed hayeoteul gamguk extracts 1, 2, and 4μg / ml treatment as shown in Fig. 2, the amount was increased.

Example  4. Glutamic acid Decarboxylase  ( glutamic acid decarboxylase , GAD ) 65/67 Expression amount  Measure

Mice were dosed orally with 100 mg / kg of gamguk extract and 40 mg / kg of pentobarbital intraperitoneally for 3 days. The hippocampus of the mouse brain was separated and crushed with an ultrasonic tissue grinder with buffer. After centrifugation of each of the pulverized 20 minutes at a rate of 20,000 X g, the amount of protein was western blotting to measure the expression of glutamic acid decarboxylase (GAD). The results are shown in Fig.

As shown in FIG. 3, the expression level of glutamic acid decarboxylase (GAD) was increased in the gakg extract administration group compared to the control group.

From the above results, it was inferred that the administration of gamguk extract increased the expression of glutamate decarboxylase and promoted the influx of chloride ions (Cl ⁻ ) in neurons, resulting in a shorter sleep induction time and longer sleep time. can do.

Example  5. Preparation of Pharmaceutical Composition

Tablets, capsules, and powders were prepared according to the following compositions, respectively.

1) tablets

Persimmon Extract 250.0mg

Lactose 500.0mg

Talc 5.0mg

Magnesium Stearate 1.0mg

2) Capsule

After sieving the mixture with the excipient and sieved, the capsule was prepared by filling the gelatin capsule according to the following composition.

Persimmon Extract 250.0mg

Starch 1500 10.0mg

Magnesium Stearate 1.0mg

3) powder

The following components were mixed by a conventional powder preparation method, put into a bag, sealed, and powder was prepared.

Persimmon Extract 250.0mg

Lactose 100.0 mg

Talc 5.0mg

Example  6. Manufacturing of dietary supplements

The following ingredients were mixed with water to prepare a beverage in a conventional manner, and adjusted to 100 ml in total, and then sterilized and cooled at 95 ° C. for 15 seconds to prepare a drink.

500.0mg of Korean soup extract

Citric Acid 1000.0mg

Gum arabic 1000.0mg

Sugar 5000.0mg

The present invention described above is not limited to the above-described embodiment and the accompanying drawings, and various substitutions, modifications, and changes are possible within the scope without departing from the technical spirit of the present invention. It will be evident to those who have knowledge of.

In Figure 1 (A) depicts the effect on the sleeping time, (B) will showing the effect on sleep induction time, in Figure 2 in a chloride ion (Cl ^-) neurons will showing the effect on the amount of , Figure 3 shows the effect on the expression level of glutamic acid decarboxylase (GAD).

Claims (4)

A composition for the prevention and improvement of insomnia, characterized in that it comprises a extract of gamgu.
The method according to claim 1,
The gamguk extract composition for the prevention and improvement of insomnia symptoms characterized in that it comprises at least 50 mg / kg in an effective amount in the composition.
Food comprising a composition for preventing and improving insomnia symptoms of claim 1.
Method of producing a composition for the prevention and improvement of insomnia, characterized in that it comprises the step of extracting, filtering, concentrating and drying the persimmon soup with an aqueous solution containing 40 to 80% by weight of lower alcohol having 1 to 4 carbon atoms.

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