KR20030031331A - Antifungal compound and its composition containing Cinnamomum cassia extract. - Google Patents

Abstract

PURPOSE: A antifungal and insecticidal composition containing an extract of Cinnamomi Cortex as a main ingredient is provided which exhibits excellent antifungal activity against Botrytis cinerea and Colletotrichum orbiculare causing major plant diseases. CONSTITUTION: Cinnamomi Cortex is crushed, followed by addition of 5 times of 70% hydrous alcohol, extraction at 4 to 40deg.C for 12hr and filtration. The antifungal and insecticidal composition contains 0.002 to 20% by weight of the Cinnamomi Cortex extract. For an example, 1kg of Cinnamomi Cortex is crushed, followed by addition of 5 times of 70% hydrous alcohol, extraction at 4 to 40deg.C for 12hr, filtration with Whatman No. 42 paper and then drying with a rotary vacuum evaporator to produce 115g of the composition.

Description

Antifungal compound and its composition containing Cinnamomum cassia extract.

The present invention relates to the use of extracts of cinnamon which is a natural product pulverized by drying the epidermis of a plant belonging to the camphor family as a natural biopesticide or freshener for phytopathogenic fungi.

Botrytis cinerea is a fungus belonging to an incomplete fungus that causes many horticultural crops such as grapes, apples, tomatoes, and strawberries, as well as a multiplicity of gray fungal diseases that occur in trees and cereals, and causes various types of enormous damage. And variously called according to the symptoms and the site of the disease. The parasitic power of gray fungal pathogen conidia is weak in the healthy part of foliage and fruit, and is easily invaded directly into tissues without scars or vitality, causing disease. In the case of grapes, the gray mold fungus invades at the end of flowering period, remains latent until the maturity of the fruit, and then develops thereafter.

Colletotrichum , a plant anthrax bacterium, is a disease that affects about 300 kinds of plants, especially apple trees, and is a high risk of developing in high temperature and high humidity environments from late July to August. Anthrax is important as a plant disease because it occurs in apples, tangerines, lambs, peppers, tomatoes, legumes, and many other crops, as well as damage to growing and post-harvest berries. Anthrax mainly occurs on leaves, stems, or fruits, forming dark spots, slightly raised edges, somewhat inward lesions, and persimmon anthracnose, which causes fall and fruit rot by pathogens.

Natural antimicrobial is a substance that suppresses microbial contamination and growth, and is widely used as food additives, pharmaceuticals, and industrial substances. Currently, hinokitiol, a cypress extract, megnonol, a magnolia extract, and grapefruit seed extract, DF -100, etc. have been developed, but only 17% of the synthetic preservatives. Until now, chemicals have been used as antifungal agents, and chemical antifungal agents have been developed and used for pathogenic fungi. However, these chemical substances are causing social problems as environmentally damaging substances, and sometimes have side effects that are harmful to the human body. Thus, a great deal of effort has been devoted to the separation of substances with excellent antifungal effects from natural products. Recently, with the development of genetic engineering technology, much research progress has been made in mass production of some newly discovered natural antifungal substances. In addition, the purification characteristics and antifungal activity of natural antifungal substances have been studied, and the development of antibiotics or natural pesticides using the same has been attempted, but the research on the application of these antifungal substances is still insufficient.

Currently used pesticides can be divided into a chemical synthesis system and natural products using natural enemies and plant extracts. Most of them are chemical synthetic pesticides, and their medicinal effects are recognized as excellent, but they are highly restricted in their use because they cause strong non-selective toxicity to human body and other ecosystems. In addition, natural insecticides and fungicides are not widely used because they are relatively weak in human toxicity but have low efficacy or low efficacy in actual application even if their efficacy is high in vitro.

The present invention searches for a new natural product having a high antifungal activity among natural products, and indicates that it can be applied as a natural biopesticide.

The present invention relates to an antifungal agent comprising cinnamon extract as a main component, and more specifically, an extract made by adding an organic solvent by drying the bark of a cinnamon tree belonging to the camphor tree and finely pulverizing, and having excellent antifungal power against phytopathogens. It relates to natural biopesticides.

In order to search for natural products with excellent antifungal activity, the main plant pathogens such as gray fungal pathogens and anthrax pathogens were used to determine the antifungal activity against various plant extracts by measuring their inhibition rate. As a result, the cinnamon extract was found to have superior antifungal activity compared to other natural extracts.

Example 1

Cinnamon extract production method of the present invention is as follows.

1 kg of Cinnamomum cassia was pulverized, and 5% of 70% hydrous alcohol was added, followed by extraction at 4-40 ° C. for 12 hours, followed by filtration using Whatman 42 filter paper, and the filtrate was dried at 65 ° C. using a rotary evaporator. To give 115 g of dry weight.

Experimental Example 1: Antifungal activity of cinnamon extract against gray fungus and anthrax

Potato dextrose agar medium containing spore suspensions of phytopathogens gray fungus and anthrax botrytis cinerea and Colletotrichum orbiculare was added to a 24weel plate, and the extract of Example 1 was dissolved in ethanol and diluted sequentially at a concentration of 10 mg / ml. After direct addition to the surface of the culture medium was incubated for 5 days at 20 ℃ to observe the growth of mycelia compared to the untreated group visually. The antifungal effects of chemical fungicides and cinnamon extracts used in combination with gray mold and anthrax were compared. The experimental results are shown in Table 1.

Table 1

Inhibitory Effects of Cinnamon Extracts on Gray Fungus and Anthrax Bacteria

Experimental results showed that the antifungal effect according to the concentration of cinnamon extract was active at the concentration of 100㎍ / ㎖ for gray fungus, and at 300㎍ / mL for anthrax. Confirmed. Antifungal activity was confirmed at a concentration similar to benomyl, the most commonly used chemical disinfectant, indicating that it could be used as a natural biopesticide.

Example 2

The compositional composition of natural biopesticides containing cinnamon extract is shown in Table 2.

[Table 2]

Experimental Example 2: Antifungal Activity Effect of Cinnamon Extract

Example 2 was used in strawberry seedlings with progressive growth of gray mold and anthrax, respectively, at concentrations of 1000 μg / ml, 800 μg / ml, 500 μg / ml, 300 μg / ml, 100 μg / ml and 50 μg / ml. After the treatment with foliar fertilization, the actual antifungal activity from 1 to 10 days was compared with the non-treated group, and the following results were obtained.

[Table 2]

Antifungal Effect of Cinnamon Extracts on Direct Treatment with Strawberry Seedlings

: Active. -: No activity.

As a result of the experiment, it was confirmed that the growth of gray fungus bacteria was inhibited at the concentration of 300 µg / ml from the second day after the cinnamon extract treatment, and the concentration of 50 µg / ml was treated from the third day after the treatment. It was confirmed that the ash fungus of strawberry seedlings can no longer grow.

Experimental Example 3 Comparison of Fruit Yields after Cinnamon Extract Treatment in Strawberry Fields

About 10 days after the treatment of Example 2, the fruit harvested at the harvester was randomly harvested, and then the decay rate was compared by comparing the untreated and treated groups.

Table 3

Comparative experiment with the ratio of cinnamon extract in the strawberry field

As a result, when the cinnamon extract was treated, the fruit was harvested significantly more than the untreated group, and the product value of the fruit was also significantly higher than the untreated group.

Example 3.

Antifungal Effect of Cinnamon Extracts on Bacterial Fungi on Strawberry Storage

Example 2 directly into the strawberry fruit at a concentration of 1000 ㎍ / ㎖, 800 ㎍ / ㎖, 500 ㎍ / ㎖, 300 ㎍ / ㎖, 100 ㎍ / ㎖, 50 ㎍ / ㎖ and stored for 1 week at room temperature In comparison, the results were observed.

Table 4

Growth Inhibitory Activities of Gray Fungus Roots by Concentration of Cinnamon Extracts during Strawberry Storage

From the second day after the treatment of cinnamon extract, antifungal activity was observed at the concentration of 500 µg / ml and from the concentration of 100 µg / ml from the third day.

Cinnamon extract of the present invention was the strongest antifungal activity in natural products, and the result of treating the cinnamon extract in real fields and seedlings, the antifungal activity against gray mold and anthrax, which is known as the causative agent of major plant diseases, was very good.

Claims (4)

Cinnamomum cassia was pulverized, and 70% hydrous alcohol, which is 5 times the weight, was added, followed by extraction at 4-40 ° C. for 12 hours, followed by filtration. Pesticide and freshener containing this filtrate According to claim 1, Pesticides and fresheners, characterized in that the cinnamon is a dried herb of the plant belonging to the camphor family The pesticide and freshening preservative according to claim 1, wherein the concentration of cinnamon extract is contained in an amount of 0.002 to 20% by weight. The additive for crops according to claim 1, wherein the concentration of the cinnamon extract is contained in an amount of 0.002 to 20% by weight.