KR20010077869A - bioreactor - Google Patents

Abstract

PURPOSE: An air-lift reactor useful for culturing microorganisms, plants and animals is provided, which is cheap and convenient to use in culturing microorganisms, plants and animals. CONSTITUTION: The air-lift reactor consists of a basis of a culturing vessel(7), the culturing vessel(5), a cover of the culturing vessel(1) which is made of transparent polymers and contains a hook pole for locking the cover(3), in the center, a bolt type of handle for tightening the cover(2), and a door of the culturing vessel(4) connected with the handle; the basis of the culturing vessel which is made of organic synthesized materials or light metals, and contains a bubble producing apparatus(6) in the center, an air inlet(6) and a medium outlet(8); and the culturing vessel made of glass.

Description

Bioreactor {bioreactor}

Fermenters used in laboratories in biotechnology and bioindustry are very complex and expensive and have limited use. This is thought to be inevitable due to the diversification of functions, but it is often not enough to use for the price, and the lack of popularity is a big disadvantage.

The main functions of the bioreactor are to control the temperature of the culture medium, to supply air, and to control the concentration of hydrogen ions. The first temperature control is the heat conduction from the base where the culture chamber is placed and the distribution of water in the double wall around the culture chamber. The temperature of the medium is controlled. Secondly, the air supply function has an inlet at the top and a base. When the medium is stirred by the force of air, an air lift type is obtained. This is more advanced than stirring the medium by the impeller. Third, hydrogen ion concentration control is the most complex function and the most expensive part of the installation.

In addition to these basic main functions, bioreactors are very expensive to deal with, but additional reactors that can be purchased at low cost will help the development of the field using them.

In the present invention, the following points are improved for the purpose of lowering the production cost by simplifying the existing ones while being easy to use.

First is the simplification of the structure.

The laboratory culture chamber must be transparent, so it is made of glass. In conventional bioreactors, if there is a lid on the top, it is made of metal, and in order to fix it to the culture chamber, a connecting rod of at least 5 mm in diameter is vertically connected to the base. This connecting rod serves to fix and protect the culture chamber but, together with the lid, blocks the rays of the culture and adversely affects the culture. On the other hand, in order not to use the lid and connecting rod, a ball-shaped or oval culture chamber may be used, but in this form, the capacity cannot be larger than 10L, and the manufacturing cost is more expensive than the tubular shape.

Therefore, a bioreactor with a culture chamber that is cylindrical, transparent at the top, and without a hinge on the side is required. In the present invention, while using a cylindrical culture chamber, it is possible to remove the hinge connecting the upper surface and the base.

Second, there is nothing caught in the culture chamber.

When the air inlet is in the top-down mode, there must be an air inlet pipe in the medium, and a separate air spreader must be installed inside to disperse the air even when the bottom-up air flows up from the base. In order to freely flow the culture, it is better not to be caught inside the culture chamber, but the existing ones inevitably stir the medium and disperse the air.

Third, the thermostat is separate.

In many cases, there is a separate environmental control room in the building, so bioreactors without temperature control can be placed here, but existing bioreactors should be attached even if they are not needed because the temperature control function is already installed in the device. . By separating the culture chamber and the temperature control device to purchase according to the user's needs, it is possible to lower the volume and manufacturing cost of the device.

1 is a front view of the present invention and the name of each part

Figure 2 is a cross section of the top cover of the bioreactor

Figure 3 is a cross section of the base of the bioreactor culture chamber

Figure 1 shows the overall configuration of the bioreactor, the center is a glass culture chamber (5), the upper surface is a transparent heat-resistant plastics (polymer composite material), the culture chamber base is opaque heat-resistant plastics or aluminum .

Figure 2 shows the structure of the door used to put the culture material into the culture chamber through the culture chamber lid (1). The center of the lid has a door (4) and the door is moved up and down by the door opening and closing handle (2). The handle is connected to the door by a spiral shaft passing through the center of the locking stand (3).

One end of the locking claw is fixed by the rotary shaft 12 and the other end is locked to the locking claw latching jaw (13). On one side of the lid there are 3-5 entrances and exits for the material in culture.

In FIG. 3, the upper surface of the culture chamber base 7 has an air dispersing plate 6 having a diameter of 5-20 cm, and an air inlet 10 and a medium discharge port 8 at the bottom thereof, and a solenoid valve 9 in front of the air inlet. Stick.

Using this device by supplying a low cost and easy to use bioreactor

Helps research and production in cell and tissue culture in microorganisms, plants and animals.

Claims (4)

In the apparatus for culturing cells and tissues of microorganisms, plants and animals, an air-lift type bioreactor comprising a combination of a culture chamber base (7), a culture chamber (5) and a culture chamber lid (1). In the center of the culture chamber lid, there is a culture chamber door (4) connected to the locking hanger (3) and the bolt-type handle (2) for fastening the door, and the bubble chamber is formed at the center of the culture chamber base (6), and the air inlet (10) and Bioreactor characterized in that there is a medium outlet (8) The bioreactor according to claim 1, wherein the culture chamber base (7) is a polymer-based organic composite or a light metal, and the culture chamber lid (1) is a transparent polymer-based organic composite and the culture chamber wall is made of glass. In a device for culturing cells and tissues of microorganisms, plants and animals, a bioreactor characterized in that a solenoid valve (9) is used to prevent the liquid in the culture chamber from leaking into the air inlet (10). In the apparatus for culturing cells and tissues of microorganisms, plants and animals, the culture chamber door (4) is in close contact by a bolt fastening knob (2), characterized in that it is rotated on the locking hanger (3) Bioreactor