KR102620858B1 - Necleic acid extraction device and cap used for nucleic acid extraction device - Google Patents

Abstract

The present invention includes a slot 200 in which a receiving groove 210 is formed into which a cartridge 20 having one or more wells (W) or an adapter 30 coupled to one or more of the cartridges 20 is inserted; One or more magnetic rods 400; A magnetic rod holder (500) coupled to the magnetic rod (400); A cap holder (600) disposed below the magnetic rod holder (500) and coupled to one or more caps (C); It is connected to the magnetic rod holder 500 and moves the magnetic rod holder 500 up and down so that the magnetic rod 400 enters the inside of the cap (C) coupled to the cap holder 600 or the cap (C) ) a first driving unit 700 that is retracted from the inside of the; It is connected to the cap holder 600 and the first driving part 700, moves the cap holder 600 and the first driving part 700 together up and down, and places the cap C coupled to the cap holder 600 in the receiving groove. Entering the well (W) of the cartridge 20 inserted into (210) or the well (W) of the cartridge 20 combined with the adapter 30 inserted into the receiving groove 210, or entering the well (W) a second driving unit 800 that is retracted from the inside; and a third driving unit 900 that moves the magnetic rod holder 500 and the cap holder 600 in a first direction or moves the slot 200 in the first direction. .
Accordingly, the cap holder 600 and the magnetic rod holder 500 can be controlled simply and stably, and damage to the nucleic acid extraction device due to malfunction can be prevented.

Description

Nucleic acid extraction device and cap used in nucleic acid extraction device {NECLEIC ACID EXTRACTION DEVICE AND CAP USED FOR NUCLEIC ACID EXTRACTION DEVICE}

The present invention relates to a nucleic acid extraction device and a cap used in the nucleic acid extraction device, and more specifically, to a nucleic acid extraction device that is simply and stably controlled and prevents damage due to malfunction, and a cap that significantly improves nucleic acid extraction efficiency. It's about.

A method of extracting nucleic acids or biological substances (proteins, etc.) from samples collected from organisms, viruses, etc. using magnetic beads is widely used.

In order to extract nucleic acids or biological substances using magnetic beads, several steps such as lysis & binding, washing, and elution must be performed, making the extraction process complex and time-consuming. . Accordingly, extraction devices that automatically perform the above various steps have been developed and used. Prior art related to this includes Korean Patent Publication No. 10-2020-0032639.

However, in the conventional nucleic acid extraction device, the magnet rod and the stirring rod are raised and lowered completely independently of each other, so control is complicated and malfunction may result in significant damage to the nucleic acid extraction device. In addition, in the conventional nucleic acid extraction device, when the magnet rod and the stirring rod are raised and lowered, they are tilted relative to each other due to vibration, etc., resulting in various malfunction problems, such as the magnet rod not being inserted into the stirring rod. In addition, when using a conventional nucleic acid extraction device, there is a problem that nucleic acid extraction efficiency is lowered due to nucleic acids becoming tangled or attached to the stirring rod.

KR 10-2020-0032639

The present invention was conceived to solve the above-mentioned problems, and the purpose of embodiments of the present invention is to provide a nucleic acid extraction device that is controlled simply and stably and prevents damage due to malfunction.

Additionally, the purpose of embodiments of the present invention is to provide a nucleic acid extraction device that performs nucleic acid extraction easily and stably.

In addition, embodiments of the present invention are a nucleic acid extraction device that stably fixes a cartridge or adapter to a slot with a simple configuration at low cost and prevents the cartridge 20 or adapter 30 from being incompletely inserted into the slot 200. The purpose is to provide.

Additionally, embodiments of the present invention aim to provide a nucleic acid extraction device in which two or more cartridges or adapters having different widths or cartridges and adapters having different widths are stably fixed by the same ball plunger.

In addition, the purpose of embodiments of the present invention is to provide a nucleic acid extraction device in which the slot is easily fixed at low cost with a simple configuration and the fully retracted state of the slot is stably maintained.

In addition, the purpose of embodiments of the present invention is to provide a nucleic acid extraction device that stably fixes the strip to the cap holder and prevents the strip from being incompletely inserted into the cap holder with a simple structure and low cost.

Additionally, embodiments of the present invention aim to provide a cap that significantly improves nucleic acid extraction efficiency.

In order to solve the above-described problem, the present invention includes a receiving groove 210 into which a cartridge 20 having one or more wells (W) or an adapter 30 coupled to one or more of the cartridges 20 is inserted. A slot 200 is formed; One or more magnetic rods 400; A magnetic rod holder (500) coupled to the magnetic rod (400); A cap holder (600) disposed below the magnetic rod holder (500) and coupled to one or more caps (C); It is connected to the magnetic rod holder 500 and moves the magnetic rod holder 500 up and down so that the magnetic rod 400 enters the inside of the cap (C) coupled to the cap holder 600 or the cap (C) ) a first driving unit 700 that is retracted from the inside of the; It is connected to the cap holder 600 and the first driving part 700, moves the cap holder 600 and the first driving part 700 together up and down, and places the cap C coupled to the cap holder 600 into the receiving groove. Entering the well (W) of the cartridge 20 inserted into (210) or the well (W) of the cartridge 20 combined with the adapter 30 inserted into the receiving groove 210, or entering the well (W) a second driving unit 800 that is retracted from the inside; and a third driving unit 900 that moves the magnetic rod holder 500 and the cap holder 600 in a first direction or moves the slot 200 in the first direction. .

In one embodiment, in the process of extracting nucleic acid using the nucleic acid extraction device 10, when the magnetic rod holder 500 rises from the cap holder 600 to its maximum height, the magnetic rod holder 500 The coupled magnetic rod 400 is inserted into the through hole 610 formed in the cap holder 600 and communicating with the inside of the cap C coupled to the cap holder 600 or the cap holder 500. It is inserted into the cap (C) coupled to .

In one embodiment, the magnetic rod holder 500, the cap holder 600, and the first driving part 700 are slidably coupled to the same first guide part GR1 extending vertically.

In one embodiment, the second driving unit 800 includes a second motor 810 and a motion conversion means (T) for converting the rotational motion of the second motor 810 into a vertical translational motion, and the cap holder. (600) is slidably coupled to the first guide part (GR1) and the second guide part (GR2), which extend up and down and are arranged side by side at a predetermined interval, and the motion conversion means (T) It is disposed between the first guide part (G1) and the second guide part (G2).

In addition, in order to solve the above-described problem, the present invention includes a cartridge 20 having one or more wells (W) or an adapter 30 coupled to one or more of the cartridges 20; A slot 200 in which a receiving groove 210 is formed into which the cartridge 20 or adapter 30 is inserted; One or more magnetic rods 400; A magnetic rod holder (500) coupled to the magnetic rod (400); A cap holder (600) disposed below the magnetic rod holder (500) and coupled to one or more caps (C); and moving the magnetic rod holder 500 up and down to cause the magnetic rod 400 to enter or retreat from the inside of the cap C coupled to the cap holder 600, and move the cap holder 600 up and down. Move the cap (C) coupled to the cap holder 600 to the well (W) of the cartridge 20 inserted into the receiving groove 210 or the adapter 30 inserted into the receiving groove 210. Entering or withdrawing from the inside of the well W of the cartridge 20, moving the magnetic rod holder 500 and the cap holder 600 in a first direction or moving the slot 200 in a first direction. Shiki provides a nucleic acid extraction device including driving units (700, 800, and 900).

One or more grooves are formed on the inner surface of the receiving groove 210 in a direction crossing the direction in which the cartridge 20 or adapter 30 is inserted into the receiving groove 210.

A first ball plunger 230 is installed in each groove, and an end of the first ball plunger 230 protrudes inside the receiving groove 210.

A first ball plunger groove 36 or a protrusion 24 is formed on the outer surface of the cartridge 20 or adapter 30, and the cartridge 20 or adapter 30 is inserted into the receiving groove 210. When inserted, the end of the first ball plunger 230 is inserted into the first ball plunger groove 36 or passes through the protrusion 24 and is located on the upper part of the protrusion 24.

In one embodiment, the receiving groove 210 includes an outer groove 212 formed on the outside and having a larger width, and an inner groove 214 formed on the inside of the outer groove 212 and having a smaller width. , a step (S) is formed on the inner surface of the receiving groove 210 by the outer groove 212 and the inner groove 214, and the first ball plunger 230 is located inside the outer groove 212. It is installed on the side.

In one embodiment, the slot 200 slides to be inserted into and extracted from the nucleic acid extraction device, and when the slot 200 is inserted into the nucleic acid extraction device, it is in contact with the slot 200 or at a predetermined distance. Facing locking protrusions 110 are provided, and the slots 200 and the locking protrusions 110 are each provided with magnets or with magnets and metal at predetermined portions that contact or face each other.

In addition, in order to solve the above-mentioned problem, the present invention provides a receiving groove 210 into which a cartridge 20 having one or more wells (W) or an adapter 30 coupled to one or more of the cartridges 20 is inserted. A slot 200 is formed; One or more magnetic rods 400; A magnetic rod holder (500) coupled to the magnetic rod (400); A strip 40 including one or more caps C into which the magnetic rod 400 is inserted and a connecting portion 42 extending in a direction intersecting the direction in which the caps C extend and connecting the caps C. ); a cap holder 600 disposed below the magnetic rod holder 500 and having one or more insertion grooves 620 into which the strip 40 is inserted; And by moving the magnetic rod holder 500 up and down, the magnetic rod 400 enters or retreats from the inside of the cap C of the strip 40 inserted into the cap holder 600, and the cap holder ( 600) is moved up and down to place the cap C of the strip 40 inserted into the cap holder 600 into the well W of the cartridge 20 inserted into the receiving groove 210 or the receiving groove 210. The cartridge 20 combined with the inserted adapter 30 is entered into or retreated from the inside of the well W, and the magnetic rod holder 500 and the cap holder 600 are moved in the first direction or moved into the slot. A nucleic acid extraction device including a driving unit (700, 800, 900) that moves (200) in a first direction is provided.

A groove or through hole is formed on the inner surface of the insertion groove 620 in a direction crossing the direction in which the strip 40 is inserted into the insertion groove 620.

A second ball plunger 630 is disposed in the groove or through hole, an end of the second ball plunger 630 protrudes inside the insertion groove 620, and a second ball plunger is in the connecting portion 42. A groove 44 is formed.

When the strip 40 is inserted into the insertion groove 620, the end of the second ball plunger 630 is inserted into the second ball plunger groove 44.

In addition, in order to solve the above-mentioned problem, the present invention provides a receiving groove 210 into which a cartridge 20 having one or more wells (W) or an adapter 30 coupled to one or more of the cartridges 20 is inserted. A slot 200 is formed; One or more magnetic rods 400; A magnetic rod holder (500) coupled to the magnetic rod (400); A cap holder (600) disposed below the magnetic rod holder (500) and coupled to one or more caps (C); and moving the magnetic rod holder 500 up and down to cause the magnetic rod 400 to enter or retreat from the inside of the cap C coupled to the cap holder 600, and move the cap holder 600 up and down. Move the cap (C) coupled to the cap holder 600 to the well (W) of the cartridge 20 inserted into the receiving groove 210 or the adapter 30 inserted into the receiving groove 210. A cap (C) coupled to the cap holder (600) of a nucleic acid extraction device including driving units (700, 800) for entering or retracting the well (W) of the cartridge (20) is provided.

The cap (C) is formed as a hollow extending up and down and open at the top, and includes a section (R) in which the width decreases toward the bottom and the rate of decrease in width gradually decreases.

According to embodiments of the present invention, the nucleic acid extraction device 10 includes a cartridge 20 having one or more wells W or a receiving groove into which an adapter 30 coupled to one or more cartridges 20 is inserted. Slot 200 in which 210 is formed; One or more magnetic rods 400; A magnetic rod holder (500) coupled to the magnetic rod (400); A cap holder (600) disposed below the magnetic rod holder (500) and coupled to one or more caps (C); It is connected to the magnetic rod holder 500 and moves the magnetic rod holder 500 up and down to allow the magnetic rod 400 to enter the inside of the cap (C) coupled to the cap holder 600 or the inside of the cap (C). A first driving unit 700 that is retracted from; It is connected to the cap holder 600 and the first driving part 700, moves the cap holder 600 and the first driving part 700 up and down together, and moves the cap C coupled to the cap holder 600 into the receiving groove 210. ) into the well W of the cartridge 20 inserted into the well W of the cartridge 20 combined with the adapter 30 inserted into the receiving groove 210, or into the inside of the well W. a second driving unit 800 that is retracted from; and a third driving unit 900 that moves the magnetic rod holder 500 and the cap holder 600 in the first direction or moves the slot 200 in the first direction.

Accordingly, when the second driving part 800 moves the cap holder 600 up and down, the magnetic rod holder 500 coupled to the first driving part 700 can also move up and down together. Accordingly, when the height of the cap holder 600 changes, the height of the magnetic rod holder 500 may also change equally. Accordingly, the cap holder 600 and the magnetic rod holder 500 can be controlled more simply and stably than when they move up and down independently of each other, and malfunctions such as misjudging the positions (heights) of the holders 500 and 600 can be prevented. It can prevent damage to the nucleic acid extraction device.

Specifically, the cap holder 600 rises together with the magnetic rod holder 500, so when the cap holder 600 is raised, the cap holder 600 or the cap (C) moves with the magnetic rod holder 500 or the magnetic rod 400. ) There is no need to control it to avoid colliding with it. In addition, since the cap holder 600 goes up and down together with the magnetic rod holder 500, the magnetic rod holder 500 always moves up and down the cap holder 600 by a predetermined distance (height). It can be located at the top. Accordingly, since the magnetic rod holder 500 can stably descend by the predetermined distance (height), there is no need to consider the position (height) of the cap holder 600 when lowering the magnetic rod holder 500.

According to an embodiment of the present invention, in the process of extracting nucleic acid using the nucleic acid extraction device 10, when the magnetic rod holder 500 rises from the cap holder 600 to the maximum height, the magnetic rod holder 500 The combined magnetic rod 400 is inserted into the third through hole 610 formed in the cap holder 600 and communicated with the inside of the cap C coupled to the cap holder 600, or the cap holder 500 ) may be inserted into the cap (C) coupled to the cap (C).

Accordingly, the cap holder 600 and the magnetic rod holder 500 move up and down together, so the end of the magnetic rod 400 can always remain inserted into the third through hole 610 or the cap C. . Accordingly, it is possible to prevent the magnetic rod 400 from being inserted into the cap C due to vibration or the like. Accordingly, nucleic acid extraction can be performed easily and stably and damage to the nucleic acid extraction device can be prevented.

According to an embodiment of the present invention, the magnetic rod holder 500, the cap holder 600, and the first driving part 700 can be slidably coupled to the same first guide part GR1 extending vertically.

Accordingly, the magnetic rod holder 500, the cap holder 600, and the first driving unit 700 can be moved by being guided by the same first guide unit GR1. Accordingly, the magnetic rod holder 500 and the cap holder 600 are relatively tilted toward each other to prevent the magnetic rod 400 from being inserted into the cap C or from damaging or pressing the cap C. In addition, damage to the nucleic acid extraction device can be prevented by the magnetic rod holder 500 and the first driving unit 700 being relatively tilted toward each other. In particular, the magnetic rod holder 500, cap holder 600, and first driving unit 700 are guided to the same first guide unit GR1, so that even if vibration is generated by the driving units 700, 800, and 900, the nucleic acid extraction device can operate stably.

According to an embodiment of the present invention, the second driving unit 800 includes a second motor 810 and a motion conversion means (T) for converting the rotational motion of the second motor 810 into a vertical translational motion, and a cap. The holder 600 extends up and down and is slidably coupled to the first guide part (GR1) and the second guide part (GR2) arranged side by side at a predetermined interval, and the motion conversion means (T) is the first guide part (T). It may be disposed between the guide part G1 and the second guide part G2.

Accordingly, the vertical force of the second driving unit 800 can be evenly transmitted to the first guide coupling part (M11) and the third guide coupling part (M21) coupled to the cap holder 600, so that the cap holder 600 It can be lifted and lowered stably by being guided by the first guide part (GR1) and the second guide part (GR2).

According to an embodiment of the present invention, a nucleic acid extraction device includes a cartridge 20 having one or more wells (W) or an adapter 30 coupled to one or more cartridges 20; A slot 200 in which a receiving groove 210 is formed into which the cartridge 20 or adapter 30 is inserted; One or more magnetic rods 400; A magnetic rod holder (500) coupled to the magnetic rod (400); A cap holder (600) disposed below the magnetic rod holder (500) and coupled to one or more caps (C); and moving the magnetic rod holder 500 up and down to allow the magnetic rod 400 to enter or retreat from the inside of the cap (C) coupled to the cap holder 600, and to move the cap holder 600 up and down to remove the cap. The cap (C) coupled to the holder 600 is connected to the well (W) of the cartridge 20 inserted into the receiving groove 210 or the cartridge 20 coupled with the adapter 30 inserted into the receiving groove 210. Drive units 700 and 800 that enter or retreat into the well W, move the magnetic rod holder 500 and the cap holder 600 in a first direction, or move the slot 200 in the first direction. 900). One or more grooves are formed on the inner surface of the receiving groove 210 in a direction crossing the direction in which the cartridge 20 or adapter 30 is inserted into the receiving groove 210, and each groove includes a first ball plunger ( 230) is installed, the end of the first ball plunger 230 protrudes inside the receiving groove 210, and the first ball plunger groove 36 or When the protrusion 24 is formed and the cartridge 20 or adapter 30 is inserted into the receiving groove 210, the end of the first ball plunger 230 is inserted into the first ball plunger groove 36 or the protrusion ( 24) and may be located on the upper part of the protrusion 24.

Accordingly, the cartridge 20 or adapter 30 can be stably fixed to the slot 200 easily and at low cost with a simple configuration. In particular, even if a lot of vibration occurs, the cartridge 20 or adapter 30 can be stably maintained completely inserted into the receiving groove 210, thereby preventing damage to the nucleic acid extraction device.

In addition, when the end of the first ball plunger 230 is inserted into the first ball plunger groove 36 of the adapter 30 or passes through the protrusion 24 of the cartridge 20 and is located on the upper part of the protrusion 24, vibration or A sound may be generated and the cartridge 20 or adapter 30 may be more strongly bound to the slot 200. It is possible to easily determine whether the cartridge 20 or adapter 30 is completely inserted into the receiving groove 210 through such vibration, sound, or change in binding force. Accordingly, it is possible to prevent damage to the nucleic acid extraction device due to incomplete insertion of the cartridge 20 or adapter 30 into the slot 200.

According to an embodiment of the present invention, the receiving groove 210 includes an outer groove 212 formed on the outside and having a larger width, and an inner groove 214 formed on the inside and having a smaller width than the outer groove 212. A step (S) is formed on the inner surface of the receiving groove 210 by the outer groove 212 and the inner groove 214, and the first ball plunger 230 is installed on the inner surface of the outer groove 212. It can be.

Accordingly, the cartridges 20 or adapters 30 having two or more different widths or the cartridges 20 and adapters 30 having different widths can be stably fixed by the same first ball plunger 230. there is. In addition, since the first ball plunger 230 is installed on the inner surface of the outer groove 212 rather than the inner surface of the inner groove 214, the first ball plunger groove 36 or the protrusion 24 is provided on the cartridge 20. There is no need to reduce the size of the well W or change the size of the body 22 of the cartridge 20 to form it.

According to an embodiment of the present invention, the slot 200 slides to be inserted into and extracted from the nucleic acid extraction device, and when the slot 200 is inserted into the nucleic acid extraction device, it contacts the slot 200 or faces it at a predetermined distance. A locking protrusion 110 is provided, and the slot 200 and the locking protrusion 110 may each be provided with a magnet or a magnet and a metal at a predetermined portion that contacts or faces each other.

Accordingly, the slot 200 can be easily fixed at low cost with a simple configuration, and the fully retracted state of the slot 200 can be stably maintained. In particular, even if a lot of vibration occurs, the fully retracted state of the slot 200 can be stably maintained, thereby preventing damage to the nucleic acid extraction device.

According to an embodiment of the present invention, the nucleic acid extraction device has a cartridge 20 having one or more wells (W) or a receiving groove 210 into which an adapter 30 coupled to one or more cartridges 20 is inserted. A slot 200 is formed; One or more magnetic rods 400; A magnetic rod holder (500) coupled to the magnetic rod (400); A strip 40 including one or more caps C into which the magnetic rod 400 is inserted and a connecting portion 42 extending in a direction intersecting the direction in which the caps C extend and connecting the caps C. ; A cap holder 600 disposed below the magnetic rod holder 500 and having one or more insertion grooves 620 into which the strip 40 is inserted. and moves the magnetic rod holder 500 up and down to cause the magnetic rod 400 to enter or retreat from the inside of the cap C of the strip 40 inserted into the cap holder 600, and move the cap holder 600 up and down. Move the cap (C) of the strip (40) inserted into the cap holder (600) to the well (W) of the cartridge (20) inserted into the receiving groove (210) or the adapter (30) inserted into the receiving groove (210). ) enters or retreats into the inside of the well (W) of the cartridge 20 coupled with ), moves the magnetic rod holder 500 and the cap holder 600 in the first direction, or moves the slot 200 in the first direction. It may include driving units 700, 800, and 900 that move it. A groove or through hole is formed on the inner surface of the insertion groove 620 in a direction crossing the direction in which the strip 40 is inserted into the insertion groove 620, and a second ball plunger 630 is disposed in the groove or through hole. , the end of the second ball plunger 630 protrudes inside the insertion groove 620, a second ball plunger groove 44 is formed in the connecting portion 42, and the strip 40 is inserted into the insertion groove 620. When inserted, the end of the second ball plunger 630 may be inserted into the second ball plunger groove 44.

Accordingly, the strip 40 can be stably fixed to the cap holder 600 easily and at low cost with a simple configuration. In particular, even if a lot of vibration occurs, the strip 40 can be stably maintained completely inserted into the insertion groove 620, thereby preventing damage to the nucleic acid extraction device.

In addition, when the end of the second ball plunger 630 is inserted into the second ball plunger groove 44 of the strip 40, vibration or sound may occur and the strip 40 is more strongly bound to the cap holder 600. It can be. It is possible to easily determine whether the strip 40 is completely inserted into the insertion groove 620 through such vibration, sound, or change in binding force. Accordingly, it is possible to prevent damage to the nucleic acid extraction device due to incomplete insertion of the strip 40 into the cap holder 600.

According to an embodiment of the present invention, the cap (C) coupled to the cap holder 600 is formed as a hollow extending up and down and open at the top, and the width decreases toward the bottom, but the rate of decrease in width gradually decreases. (R) may be included.

Accordingly, when the cap C is moved up and down, the sample, magnetic beads, lysis buffer, and wash buffer contained in the lower part of the cap C and the well W are removed. Friction with the buffer or elution buffer may increase. Accordingly, even if nucleic acids are tangled or attached to the cap (C), they are easily released by friction and fall off the cap (C), so nucleic acid extraction efficiency can be significantly improved.

In addition to the above-described effects, specific effects of the present invention are described below while explaining specific details for carrying out the invention.

Figures 1 and 2 are perspective views showing a nucleic acid extraction device according to an embodiment of the present invention, and Figure 3 is a state diagram showing a state in which the door is opened and the slot is pulled out of the nucleic acid extraction device of Figure 1.
Figures 4 and 5 are perspective views showing the inside of a nucleic acid extraction device according to an embodiment of the present invention, and Figure 6 is a state diagram showing a state in which a slot is extracted from the nucleic acid extraction device of Figure 4.
7 to 14 are a perspective view (FIG. 7, 8), top view (FIG. 9), bottom view (FIG. 10), front view (FIG. 11), and rear view (FIG. 12) showing the slot of FIGS. 3 to 6. and cross-sectional views (FIGS. 13 and 14). Figure 15 is a cross-sectional view showing a state in which the slot is inserted into the nucleic acid extraction device 10 and the bottom of the slot faces the chin.
Figures 16 to 19 are a bottom perspective view, a top view, a bottom view, and a front view of a cartridge having multiple (8 x 12) wells according to an embodiment of the present invention.
20 to 24 are a perspective view, top view, bottom view, front view, and rear view of an adapter according to an embodiment of the present invention.
25 to 28 are a perspective view, plan view, front view, and cross-sectional view of a cap holder according to an embodiment of the present invention.
29 to 32 are a bottom perspective view, top view, front view, and side view of a strip according to an embodiment of the present invention.
Figure 33 is a view showing a state in which the strip of Figures 29 to 32 is inserted into the cap holder and the cap of the strip is inserted into the well of the cartridge.
Figures 34 and 35 are a perspective view and a front view showing a magnetic rod holder combined with a magnetic rod according to an embodiment of the present invention.
Figure 36 is a diagram showing a state in which the magnetic rod coupled to the magnetic rod holder is inserted into the cap in the state of Figure 33.
Figures 37 and 38 are perspective views showing main components related to the second driving unit according to an embodiment of the present invention.
Figure 39 is an exploded perspective view of the cap holder in Figure 37 separated.
FIGS. 40 to 42 are exploded perspective views, front views, and rear views of the configuration in FIG. 39 excluding the cap holder.
Figures 43 and 44 are perspective views showing main components related to the first driving unit according to an embodiment of the present invention.
Figure 45 is an exploded perspective view of the magnetic rod holder in Figure 43 separated.
Figures 46 to 48 are an exploded perspective view, front view, and rear view of the configuration in Figure 45 excluding the magnetic rod holder.
Figure 49 is a diagram showing the operating states of the cap holder, magnetic rod, and magnetic rod holder in the nucleic acid extraction device according to an embodiment of the present invention.
Figures 50 and 51 are perspective views showing main components related to the third driving unit according to an embodiment of the present invention.
Figure 52 is an exploded perspective view of Figure 51.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

The present invention is not limited to the embodiments disclosed below, but may be subject to various changes and may be implemented in various different forms. This example is provided solely to ensure that the disclosure of the present invention is complete and to fully inform those skilled in the art of the scope of the invention. Therefore, the present invention is not limited to the embodiments disclosed below, but substitutes or adds to the configuration of one embodiment and the configuration of another embodiment, as well as all changes and equivalents included in the technical spirit and scope of the present invention. It should be understood to include substitutes.

The attached drawings are only for easy understanding of the embodiments disclosed in this specification, and the technical idea disclosed in this specification is not limited by the attached drawings, and all changes, equivalents, and changes included in the spirit and technical scope of the present invention are not limited. It should be understood to include water or substitutes. In the drawings, components may be expressed exaggeratedly large or small in size or thickness for convenience of understanding, etc., but the scope of protection of the present invention should not be construed as limited due to this.

The terms used in this specification are only used to describe specific implementations or examples, and are not intended to limit the invention. And singular expressions include plural expressions, unless the context clearly dictates otherwise. In the specification, terms such as ~include, ~consist of, etc. are intended to indicate the existence of features, numbers, steps, operations, components, parts, or a combination thereof described in the specification. In other words, terms such as ~include, ~consist, etc. in the specification. It should be understood that this does not exclude in advance the presence or addition of one or more other features, numbers, steps, operations, components, parts or combinations thereof.

Terms containing ordinal numbers, such as first, second, etc., may be used to describe various components, but the components are not limited by the terms. The above terms are used only for the purpose of distinguishing one component from another.

When a component is said to be "connected" or "connected" to another component, it is understood that it may be directly connected to or connected to the other component, but that other components may exist in between. It should be. On the other hand, when it is mentioned that a component is “directly connected” or “directly connected” to another component, it should be understood that there are no other components in between.

When a component is referred to as being "on top" or "below" another component, it should be understood that not only is it placed directly on top of the other component, but there may also be other components in between. .

Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by a person of ordinary skill in the technical field to which the present invention pertains. Terms defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related technology, and unless explicitly defined in the present application, should not be interpreted in an ideal or excessively formal sense. No.

The nucleic acid extraction device disclosed in the following examples will be examined in more detail with reference to each drawing.

Figures 1 and 2 are perspective views showing a nucleic acid extraction device according to an embodiment of the present invention, and Figure 3 is a state diagram showing a state in which the door is opened and the slot is pulled out of the nucleic acid extraction device of Figure 1. Figures 4 and 5 are perspective views showing the inside of a nucleic acid extraction device according to an embodiment of the present invention, and Figure 6 is a state diagram showing a state in which a slot is extracted from the nucleic acid extraction device of Figure 4.

1 to 6, the nucleic acid extraction device 10 according to one embodiment includes a base 100, a slot 200, a main frame 300, a magnetic rod 400, a magnetic rod holder 500, Cap holder 600, first driving unit 700, second driving unit 800, third driving unit 900, power supply unit 1000, control unit 1100, housing 1200, door 1300, display unit It may include (1400), an input unit (1500), and a communication unit (1600).

[Base]

The base 100 may have a plate shape and may be fixed to the floor (FIGS. 4 to 6).

All of the above-described configurations can be installed on the base 100.

Additionally, a locking protrusion 110 may be formed on the base 100 (FIGS. 5 and 6).

The locking protrusion 110 may be in contact with the slot 200 when the slot 200 is inserted, or may face it at a predetermined distance. The stopping protrusion 110 may restrict entry into the slot 200.

The locking protrusion 110 may be provided with a magnet (M2) or iron at a predetermined portion that contacts or faces the slot 200. Additionally, a sensor 112 that detects entry into the slot 200 may be installed on the stopping protrusion 110. In this regard, it will be described later in FIG. 15.

A third guide part GR3, a fourth guide part GR4, a fifth guide part GR5, and a sixth guide part GR6 may be installed in the base 100. The third/fourth/fifth/sixth guide parts (GR3, GR4, GR5, GR6) may be guide rails.

The third guide part GR3 and the fourth guide part GR4 may extend in a first direction (eg, left and right direction). The third guide part GR3 and the fourth guide part GR4 may guide the main frame 300 to move in the first direction. In this regard, it will be described later.

The fifth guide part GR5 and the sixth guide part GR6 may guide the slot 200 to be withdrawn and retracted in a second direction (eg, forward and backward).

[slot]

7 to 14 are a perspective view (FIG. 7, 8), top view (FIG. 9), bottom view (FIG. 10), front view (FIG. 11), and rear view (FIG. 12) showing the slot of FIGS. 3 to 6. and cross-sectional views (FIGS. 13 and 14). Figure 15 is a cross-sectional view showing a state in which the slot is inserted into the nucleic acid extraction device 10 and the bottom of the slot faces the chin.

The slot 200 may be installed on the base 100 to be retractable and withdrawn in a second direction (eg, front-to-back direction). Specifically, for example, the slot 200 may be slidably coupled with the fifth guide part GR5 and the sixth guide part GR6 installed to extend in the second direction on the base 100 (Figure 3 to Figure 6).

7 to 14, the slot 200 may be configured to include a receiving groove 210, a heater 220, a first ball plunger 230, and a stopper 240.

A cartridge 20 or an adapter 30 may be inserted into the receiving groove 210. Here, the cartridge 20 and adapter 30 will be described later.

The receiving groove 210 is formed on the outside and has a large width (e.g., the width in the second direction), and the outer groove 212 is formed on the inside of the outer groove 212 and has a width (e.g., the width in the second direction). It may be configured to include a small inner groove 214.

A step S may be formed on the inner surface of the receiving groove 210 by the outer groove 212 and the inner groove 214.

Accordingly, the cartridge 20 or adapter 30 with a large width can be fixed in contact with the inner surface of the outer groove 212, and the cartridge 20 or adapter 30 with a small width can be fixed to the inside of the inner groove 214. Since it can be fixed in contact with the side, two or more cartridges 20 or adapters 30 with different widths, or cartridges 20 and adapters 30 with different widths, can be inserted into the same slot 200 to stably It can be fixed. In particular, when extracting nucleic acids, instead of using the cartridge 20 with a large number of wells (W) for the convenience, efficiency, or cost reduction of nucleic acid extraction, the cartridge 20 with a small number of wells (W) is used. It is necessary to use it by combining it with the adapter 30. However, in general, the width of the adapter 30 is larger than the width of the cartridge 20 equipped with a plurality of wells W, so the slot 200 must be changed depending on the sizes of the adapter 30 and the cartridge 20. There was. In the present invention, the receiving groove 210 includes an outer groove 212 and an inner groove 214, and a step S is formed on the inner surface of the receiving groove 210, so that a plurality of slots 200 can be accommodated in the same slot 200. Since both the cartridge 20 with wells (W) and the adapter 30 combined with the cartridge 20 with a small number of wells (W) can be inserted and stably supported, convenience and efficiency are remarkable when extracting nucleic acids. improvements can be made and costs can be reduced.

The heater 220 may be provided on the inner bottom surface of the receiving groove 210. The heater 220 may be provided only in the rows (e.g., rows 1 and 4, 5 and 8, and 9 and 12) to which the well W, where a step requiring heating is performed among the nucleic acid extraction steps, belongs.

The heater 220 may be equipped with a temperature sensor (not shown) for each row provided with the heater 220, and the temperature may be individually controlled for each row provided with the heater 220.

The first ball plunger 230 may be installed on the inner surface of the receiving groove 210. Specifically, for example, the inner surface of the receiving groove 210 has a direction (e.g., a second direction) that intersects the direction in which the cartridge 20 or adapter 30 is inserted into the receiving groove 210 (e.g., downward). One or more grooves may be formed, and a first ball plunger 230 may be installed in each groove. The end of the first ball plunger 230 may protrude inside the receiving groove 210 (FIGS. 7 to 9, 13, and 14).

When the cartridge 20 or adapter 30 is completely inserted into the receiving groove 210, the end of the first ball plunger 230 is inserted into the first ball plunger groove 36 or passes through the protrusion 24 to form a protrusion ( It can be located at the top of 24). The first ball plunger groove 36 and the protrusion 24 will be described later.

Accordingly, the cartridge 20 or adapter 30 can be stably fixed to the slot 200 easily and at low cost with a simple configuration. In particular, since the nucleic acid extraction device generates a lot of vibration due to the driving units 700, 800, and 900, it is difficult to maintain the cartridge 20 or adapter 30 fully inserted into the receiving groove 210. If the cartridge 20 or adapter 30 is not fully inserted into the receiving groove 210, the nucleic acid extraction device may be damaged. In the present invention, by providing the first ball plunger 230 in the slot 200, the cartridge 20 or adapter 30 can be stably maintained fully inserted into the receiving groove 210 even if a lot of vibration occurs. It can prevent damage to the nucleic acid extraction device.

In addition, when the end of the first ball plunger 230 is inserted into the first ball plunger groove 36 of the adapter 30 or passes through the protrusion 24 of the cartridge 20 and is located on the upper part of the protrusion 24, vibration or A sound may be generated and the cartridge 20 or adapter 30 may be more strongly bound to the slot 200. It is possible to easily determine whether the cartridge 20 or adapter 30 is completely inserted into the receiving groove 210 through such vibration, sound, or change in binding force. Accordingly, it is possible to prevent damage to the nucleic acid extraction device due to incomplete insertion of the cartridge 20 or adapter 30 into the slot 200.

The first ball plunger 230 may be installed on the inner surface of the outer groove 212.

Accordingly, the cartridges 20 or adapters 30 having two or more different widths or the cartridges 20 and adapters 30 having different widths can be stably fixed by the same first ball plunger 230. there is. In addition, since the first ball plunger 230 is installed on the inner surface of the outer groove 212 rather than the inner surface of the inner groove 214, the first ball plunger groove 36 or the protrusion 24 is provided on the cartridge 20. There is no need to reduce the size of the well W or change the size of the body 22 of the cartridge 20 to form it.

The stopper 240 may be formed to protrude downward from the lower surface of the slot 200. The stopper 240 may be provided with one or more magnets (M1). For example, one or more holes may be formed in a predetermined area on the rear of the stopper 240, and a magnet M1 may be installed in each hole (FIGS. 8, 10 to 12, and 14). .

Referring to FIG. 15, the stopper 240 may be in contact with the stopping protrusion 110 when the slot 200 is completely retracted or may face it at a predetermined distance. The slot 200 and the locking protrusion 110 may each be provided with magnets M1 and M2 at predetermined portions that contact or face each other, as shown in FIG. 15, or, unlike FIG. 15, magnets and metal may be provided, respectively.

Accordingly, the slot 200 can be easily fixed at low cost with a simple configuration, and the fully retracted state of the slot 200 can be stably maintained. In particular, since the nucleic acid extraction device generates a lot of vibration due to the driving units 700, 800, and 900, it is difficult to maintain the slot 200 in a fully retracted state. If the slot 200 leaves the fully retracted state, the nucleic acid extraction device may be damaged. In the present invention, by providing the slot 200 with a magnet (M1) or iron, the fully retracted state of the slot 200 can be stably maintained even if a lot of vibration occurs, thereby preventing damage to the nucleic acid extraction device.

Meanwhile, a sensor 112 that detects entry into the slot 200 may be installed on the stopping protrusion 110. For example, a distance sensor or a magnetic sensor may be installed on the locking protrusion 110 and detect whether the slot 200 is completely retracted.

The sensor 112 that detects entry into the slot 200 may be connected to the control unit 1100. The control unit 1100 receives information from the sensor 112 and can prevent the nucleic acid extraction device 10 from operating before the slot 200 is completely retracted. Accordingly, damage to the nucleic acid extraction device 10 can be prevented.

[cartridge]

Figures 16 to 19 are a bottom perspective view, a top view, a bottom view, and a front view of a cartridge having multiple (8 x 12) wells according to an embodiment of the present invention.

16 to 19, the cartridge 20 may include a body portion 22 and a protrusion 24.

The body portion 22 may have one or more wells (W). A hollow with an open top may be formed in the well W. For example, a sample, magnetic bead, lysis buffer, wash buffer, elution buffer, etc. can be accommodated in the hollow.

Each nucleic acid extraction step may be performed in each well (W), and the same nucleic acid extraction step may be performed in wells (W) belonging to the same row.

In addition, in order to perform nucleic acid extraction, for example, four steps, including a lysis step, a first washing step, a second washing step, and an elution step, must be performed, so only four rows of wells (W) may be needed. Accordingly, in the cartridge 20 provided with 12 rows of wells W as shown in the drawing, the same nucleic acid extraction step can be performed on the wells W belonging to the 4n+1 (n=0, 1, 2)-th row. For example, the same nucleic acid extraction step (lysis step) may be performed in wells (W) belonging to rows 1, 5, and 9.

When the cartridge 20 is inserted into the slot 200, wells W in which steps requiring heating among the nucleic acid extraction steps are performed, such as wells W in rows 1 and 4, rows 5 and 8, rows 9 and 12, ) can only be placed on top of the heater 220. Here, steps requiring heating may be, for example, the lysis step (step 1) and the illusion step (step 4).

The body portion 22 has a smaller width in the second direction than the adapter 30, so that it does not contact the inner surface of the outer groove 212 among the receiving grooves 210 of the slot 200, but rather touches the inner surface of the inner groove 214. You can only access it. However, it is not limited to this configuration.

The protrusion 24 may be formed on the outer surface of the cartridge 20. While inserting the cartridge 20 into the receiving groove 210 of the slot 200, the protrusion 24 may press the end of the first ball plunger 230. After the cartridge 20 is completely inserted into the receiving groove 210 of the slot 200, the protrusion 24 passes through the end of the first ball plunger 230 and is located below the end of the first ball plunger 230. can do.

Accordingly, even if the width of the body portion 22 of the cartridge 20 having a plurality of wells (W) (8 can be stably fixed by interfering with the first ball plunger 230, and the cartridge 20 can be maintained fully inserted into the slot 200.

However, it is not limited to this configuration. That is, unlike the drawing, a first ball plunger groove 36 may be formed on the outer surface of the cartridge 20 instead of the protrusion 24.

Additionally, ribs L may be formed on the outer surface of the cartridge 20. The rib (L) may be inserted into the rib groove (LH) formed in the slot (200). Accordingly, the cartridge 20 can be stably fixed to the slot 200.

The ribs L and the rib grooves LH may be formed at positions that do not correspond to each other in the first or second direction. Accordingly, incorrectly inserting the cartridge 20 into the slot 200 can be prevented.

[adapter]

20 to 24 are a perspective view, top view, bottom view, front view, and rear view of an adapter according to an embodiment of the present invention.

20 to 24, the adapter 30 may include a first through hole 32, a second through hole 34, and a first ball plunger groove 36.

The first through hole 32 may be a hollow hole penetrating upward and downward and may be formed of one or more. A cartridge 20 having a small number (eg, four) wells W may be installed through the first through hole 32 . Accordingly, the adapter 30 may be combined with one or more cartridges 20 having a small number (eg, four) of wells W.

The first through hole 32 may not be formed adjacent to another first through hole 32, and a second through hole 34 may be disposed between the first through holes 32. Accordingly, a plurality of cartridges (e.g., 8) having a small number (e.g., 4) of wells W can be installed sufficiently spaced apart in the adapter 30 to prevent samples, etc. from being mixed due to vibration, etc. It can be prevented.

The second through hole 34 may be a hollow hole penetrating upward and downward and may be formed in one or more. A cap C and a magnetic rod 400, which will be described later, may penetrate the second through hole 34 without the cartridge 20 being inserted. That is, nucleic acid extraction may not be performed in the space where the second through hole 34 is formed, and the cap C and magnetic rod 400 may only move up and down.

Accordingly, in order to extract nucleic acids from a plurality of wells (W) (e.g., 8 Even if the provided nucleic acid extraction device is used as is, nucleic acids can be extracted from a small number of wells (e.g., 4 x 8) (W). At this time, among the total (e.g., 24 caps C) and magnetic rods 400, only a small number (e.g., 4 After being used, the rest can only move up and down through the second through hole (34).

Meanwhile, the first through hole 32 in which the cartridge 20 is not installed may have the same function and effect as the second through hole 34.

The first ball plunger groove 36 may be formed on the outer surface of the adapter 30. While inserting the adapter 30 into the receiving groove 210 of the slot 200, the outer surface of the adapter 30 may press the end of the first ball plunger 230. After the adapter 30 is completely inserted into the receiving groove 210 of the slot 200, the end of the first ball plunger 230 may be inserted into the first ball plunger groove 36.

Accordingly, the adapter 30 can be stably fixed by interfering with the first ball plunger 230 easily and at low cost with a simple configuration, and the adapter 30 can be maintained fully inserted into the slot 200. .

However, it is not limited to this configuration. That is, unlike the drawing, a protrusion 24 may be formed on the outer surface of the adapter 30 instead of the first ball plunger groove 36.

The outer surface of the adapter 30 may be in contact with the inner surface of the outer groove 212 of the receiving groove 210 of the slot 200. Additionally, the outer surface of the adapter 30 may also contact the inner surface of the inner groove 214.

[mainframe]

The main frame 300 may include a bottom frame 310, a central frame 320, and side frames 330 and 340, as shown in FIGS. 4 to 6.

The bottom frame 310 may be connected to the third driving unit 900 and may be slidably coupled to the third/fourth guide units GR3 and GR4. The floor frame 310 can move in the first direction by the third driving unit 900. This will be described later.

The central frame 320 may be combined with the bottom frame 310 and may extend up and down. A magnetic rod holder 500, a cap holder 600, a first driving part 700, a second driving part 800, and first/second guide parts GR1 and GR2 may be installed in the central frame 320. .

The first guide part GR1 and the second guide part GR2 may extend in the vertical direction and may be arranged side by side at a predetermined interval. The first guide part GR1 or the second guide part GR2 may guide the magnetic rod holder 500, the cap holder 600, and the first driving part 700 to move in the vertical direction. In this regard, it will be described later.

The first/second guide parts GR1 and GR2 may be guide rails.

Hereinafter, the cap holder 600, the strip 40, the magnetic rod 400, the magnetic rod holder 500, the second driving part 800, the first driving part 700, and the third driving part 900 will be looked at in order. see.

[Cap holder]

25 to 28 are a perspective view, plan view, front view, and cross-sectional view of a cap holder according to an embodiment of the present invention.

The cap holder 600 is disposed below the magnetic rod holder 500 (FIGS. 3 to 6) and may be combined with one or more caps (C). For example, the cap holder 600 may be combined with a strip 40 having one or more caps C. Here, the cap C and the strip 40 will be described later.

The cap holder 600 can move up and down by the second driving unit 800. Additionally, the cap holder 600 can move in the first direction by the third driving unit 900. The driving unit will be described later.

The cap holder 600 may include a third through hole 610, an insertion groove 620, and a second ball plunger 630.

The third through hole 610 may be a hollow hole penetrating upward and downward and may be formed in one or more. The third through hole 610 may communicate with the interior of the cap C coupled to the cap holder 600.

The magnetic rod 400 can enter or retreat from the interior of the cap C coupled to the cap holder 600 through the third through hole 610. Specifically, for example, the magnetic rod 400 may enter or retreat from the inside of the cap C of the strip 40 inserted into the cap holder 600 through the third through hole 610.

The insertion groove 620 may be formed in a second direction, for example, and may be hollow with one end (eg, front end) in the second direction open. The insertion groove 620 may communicate with the third through-hole 610, and at least a portion of the insertion groove 620 may overlap the third through-hole 610.

Additionally, one or more insertion grooves 620 may be formed and may be formed side by side in the first direction. A strip 40 may be inserted into each insertion groove 620.

The second ball plunger 630 may be provided on the inner surface of the insertion groove 620. Specifically, for example, the inner surface of the insertion groove 620 is grooved or penetrated in a direction (e.g., up and down) that intersects the direction in which the strip 40 is inserted into the insertion groove 620 (e.g., the second direction). A hole may be formed, and a second ball plunger 630 may be placed in the groove or through hole. The end of the second ball plunger 630 may protrude inside the insertion groove 620 (FIGS. 27 and 28).

When the strip 40 is completely inserted into the insertion groove 620, the end of the second ball plunger 630 may be inserted into the second ball plunger groove 44 of the strip 40. The second ball plunger groove 44 will be described later.

Accordingly, the strip 40 can be stably fixed to the cap holder 600 easily and at low cost with a simple configuration. In particular, since the nucleic acid extraction device generates a lot of vibration due to the driving units 700, 800, and 900, it is difficult to maintain the strip 40 completely inserted into the insertion groove 620. If the strip 40 is not fully inserted into the insertion groove 620, the nucleic acid extraction device may be damaged. In the present invention, by providing the second ball plunger 630 in the cap holder 600, the strip 40 can be stably maintained completely inserted into the insertion groove 620 even if a lot of vibration occurs, so that the nucleic acid extraction device It can prevent damage.

In addition, when the end of the second ball plunger 630 is inserted into the second ball plunger groove 44 of the strip 40, vibration or sound may occur and the strip 40 is more strongly bound to the cap holder 600. It can be. It is possible to easily determine whether the strip 40 is completely inserted into the insertion groove 620 through such vibration, sound, or change in binding force. Accordingly, it is possible to prevent damage to the nucleic acid extraction device due to incomplete insertion of the strip 40 into the cap holder 600.

[strip]

29 to 32 are a bottom perspective view, top view, front view, and side view of a strip according to an embodiment of the present invention. Figure 33 is a view showing a state in which the strip of Figures 29 to 32 is inserted into the cap holder and the cap of the strip is inserted into the well of the cartridge.

29 to 33, the strip 40 may include a cap C, a connecting portion 42, and a second ball plunger groove 44.

One or more caps C (eg, 8) may be provided. The cap (C) may extend up and down. A hollow top may be formed in the cap (C).

A magnetic rod 400 may be inserted into the cap (C). The cap C can pass the magnetic flux of the magnetic rod 400 entered inside and may be made of a non-magnetized material.

The cap (C) is inserted into the well (W) of the cartridge 20 inserted into the receiving groove 210 or the inside of the well (W) of the cartridge 20 combined with the adapter 30 inserted into the receiving groove 210. It can be entered or retreated (Figure 33).

The cap C may repeatedly enter and withdraw from the interior of the well W. Accordingly, the cap (C) may be a stirring rod.

The cap (C) may include a section (R) in which the width decreases toward the bottom and the rate of decrease in width gradually decreases. For example, the lower end of the cap C may have a shape other than a streamlined shape.

Accordingly, when the cap C is moved up and down, the sample, magnetic beads, lysis buffer, and wash buffer contained in the lower part of the cap C and the well W are removed. Friction with the buffer or elution buffer may increase. Accordingly, even if nucleic acids are tangled or attached to the cap (C), they are easily released by friction and fall off the cap (C), so nucleic acid extraction efficiency can be significantly improved.

Meanwhile, unlike the drawing, each cap C may be directly coupled to the cap holder 600 without the strip 40, or may be integrally coupled with the cap holder 600.

The connection portion 42 may extend in a direction intersecting the direction in which the cap C extends. Additionally, the connection portion 42 can connect one or more caps (C). The connection portion 42 may constitute the upper end of the strip 40. The connection portion 42 may be inserted into the insertion groove 620 of the cap holder 600.

The second ball plunger groove 44 may be formed in the connection portion 42. For example, the second ball plunger groove 44 may be formed on the upper surface of the front end of the connection portion 42.

While inserting the strip 40 into the insertion groove 620 of the cap holder 600, the connecting portion 42 of the strip 40 may press the end of the second ball plunger 630. After the strip 40 is completely inserted into the insertion groove 620 of the cap holder 600, the end of the second ball plunger 630 may be inserted into the second ball plunger groove 44.

Accordingly, the strip 40 can be stably fixed by interfering with the second ball plunger 630 easily and at low cost with a simple configuration, and the strip 40 can be completely inserted into the insertion groove 620 of the cap holder 600. The status quo can be maintained.

[Magnetic rod, magnetic rod holder]

Figures 34 and 35 are a perspective view and a front view showing a magnetic rod holder combined with a magnetic rod according to an embodiment of the present invention. Figure 36 is a diagram showing a state in which the magnetic rod coupled to the magnetic rod holder is inserted into the cap in the state of Figure 33.

34 and 36, the magnetic rod 400 may be coupled to the magnetic rod holder 500.

The magnetic rod 400 may enter the interior of the cap C coupled to the cap holder 600 or may be withdrawn from the interior of the cap C. At this time, in order for the magnetic rod 400 to enter the inside of the cap C, the magnetic rod 400 may penetrate the third through hole 610 of the cap holder 600.

When the magnetic rod 400 enters the inside of the cap (C), the magnetic bead contained in the well (W) may stick to the cap (C), and when the magnetic rod 400 retreats from the inside of the cap (C), the cap The magnetic bead stuck to (C) can be separated from the cap (C). Accordingly, the magnetic rod 400 can be used to move the magnetic beads to another well (W). The nucleic acid extraction step can be performed sequentially by using the magnetic rod 400 to move the magnetic beads to the wells (W) of the neighboring row.

The magnetic rod holder 500 may be combined with one or more magnetic rods 400. The magnetic rod holder 500 may be placed on the cap holder 600.

The magnetic rod holder 500 can move up and down by the first driving part 700 and the second driving part 800. Additionally, the magnetic rod holder 500 can move in the first direction by the third driving unit 900. The driving unit will be described later.

[Second District East]

Figures 37 and 38 are perspective views showing main components related to the second driving unit according to an embodiment of the present invention. Figure 39 is an exploded perspective view of the cap holder in Figure 37 separated. FIGS. 40 to 42 are exploded perspective views, front views, and rear views of the configuration in FIG. 39 excluding the cap holder.

The second driving unit 800 may be installed on the main frame 300.

The second driving unit 800 may be connected to the cap holder 600 and the first driving unit 700 and may move the cap holder 600 and the first driving unit 700 up and down together.

Accordingly, the second driving unit 800 connects the cap C coupled to the cap holder 600 to the well W of the cartridge 20 inserted into the receiving groove 210 of the slot 200 or the slot 200. The cartridge 20 coupled with the adapter 30 inserted into the receiving groove 210 may enter the inside of the well W or be withdrawn from the well W.

Specifically, for example, the second driving unit 800 includes a second motor 810, a first driving pulley 820a, a first driven pulley 820b, a first belt 830, and a second coupler 840a, 840b. , 840c).

The second motor 810 may be installed on the floor frame 310 or the central frame 320. The rotation axis of the second motor 810 may be coupled to the first driving pulley 820a.

The first driving pulley 820a and the first driven pulley 820b may be installed vertically on the central frame 320 and may be connected to each other through the first belt 830.

The first belt 830 may have irregularities formed on one surface facing the pulleys 820a and 820b and may be coupled to the second coupler 840.

When the first driving pulley (820a) is rotated by the second motor (810), the unevenness of the first belt (830) between the first driving pulley (820a) and the first driven pulley (820b) can be raised and lowered.

In this way, the first driving pulley 820a, the first driven pulley 820b, and the first belt 830 may be a motion conversion means (T) that converts the rotational motion of the second driving part 600 into a vertical translational motion. .

The second couplers 840a, 840b, and 840c may be combined with the first belt 830, the cap holder 600, and the first motor 710.

Specifically, for example, the second couplers (840a, 840b, 840c) are in contact with one surface of the first belt 830 on which irregularities are formed, and the first coupler on which irregularities are formed that engage or interfere with the irregularities of the first belt 830 Member 840a, a second coupling member 840b in contact with the other surface of the first belt 830, coupled with the first coupling member 840a, and coupled with the cap holder 600 and the third coupling member 840c, It may include a second coupling member 840b and a third coupling member 840c coupled to the first motor 710.

Accordingly, the second couplers 840a, 840b, and 840c can move the cap holder 600 and the first motor 710 up and down. When the first motor 710 moves up and down, the entire first driving unit 700 can move up and down. In this regard, it will be described later.

The second couplers 840a, 840b, and 840c may be coupled to the first guide coupling portion M11 and the second guide coupling portion M12 that are slidably coupled to the first guide portion GR1. Specifically, the first guide coupling portion (M11) may be coupled to the portion of the second coupling member (840b) where the second coupling member (840b) and the cap holder 600 are coupled, and the second guide coupling portion (M12) ) may be coupled to a portion of the second coupling member 840b where the second coupling member 840b and the third coupling member 840c are coupled.

In this way, since the second couplers (840a, 840b, 840c) are coupled to the two guide coupling parts (M11, M12) sliding on the same guide part (GR1), the cap holder 600 and the first driving part 700 It can move up and down stably. In addition, since the two guide coupling parts (M11, M12) coupled to the second coupler (840a, 840b, 840c) are located close to the cap holder 600 and the first driving part 700, the cap holder 600 and the first driving part 700 The driving unit 700 can move up and down more stably. In particular, since the cap holder 600 can quickly and stably vibrate up and down, stirring work can be performed effectively and stably.

Meanwhile, the cap holder 600 coupled to the second couplers 840a, 840b, and 840c may be slidably coupled not only to the first guide part GR1 but also to the second guide part GR2. For example, the cap holder 600 may be coupled to the third guide coupling portion M21 that is slidably coupled to the second guide portion GR2.

Accordingly, since the cap holder 600 is guided by the first and second guide parts GR1 and GR2 arranged side by side at a predetermined interval, the cap holder 600 can be stably raised and lowered without tilting to one side. there is. In particular, since the cap holder 600 can quickly and stably vibrate up and down, stirring work can be performed effectively and stably. Additionally, even if the size or width of the cap holder 600 is large, the cap holder 600 can be stably raised and lowered without tilting to one side.

At this time, the motion conversion means (T) of the above-described second driving unit (800) may be disposed between the first guide unit (G1) and the second guide unit (G2). For example, the first driving pulley 820a, the first driven pulley 820b, and the first belt 830 may be disposed between the first guide part G1 and the second guide part G2.

Accordingly, the vertical force of the second driving unit 800 can be evenly transmitted to the first guide coupling part (M11) and the third guide coupling part (M21) coupled to the cap holder 600, so that the cap holder 600 It can be lifted and lowered stably by being guided by the first guide part (GR1) and the second guide part (GR2).

Meanwhile, a first sensor (S1) may be placed in the main frame 300, the cap holder 600, or the second driving part 800 to set the reference height of the cap holder 600 and the second driving part 800. there is. The first sensor S1 may be connected to the control unit 1100.

Specifically, for example, as shown in FIGS. 38 and 42, the protrusion P1 may be formed on the third coupling member 840c, and the first sensor S1 that detects the protrusion P1 may be connected to the central frame 320. It may be placed at a predetermined height (for example, the highest height of the cap holder and the second driving unit). When the third coupling member 840c moves up and down by the second motor 810 and the protrusion P1 is positioned at the predetermined height, the first sensor S1 detects the protrusion P1 and the control unit 1100 ), and the control unit 1100 can set a reference position (reference height) for controlling the second motor 810.

[First District East]

Figures 43 and 44 are perspective views showing main components related to the first driving unit according to an embodiment of the present invention. Figure 45 is an exploded perspective view of the magnetic rod holder in Figure 43 separated. Figures 46 to 48 are an exploded perspective view, front view, and rear view of the configuration in Figure 45 excluding the magnetic rod holder.

The first driving unit 700 may be installed on the central frame 320 to be able to move up and down.

The first driving unit 700 may be connected to the magnetic rod holder 500 and may move the magnetic rod holder 500 up and down.

Accordingly, the first driving unit 700 causes the magnetic rod 400 coupled to the magnetic rod holder 500 to enter the interior of the cap C coupled to the cap holder 600 or to retreat from the interior of the cap C. You can do it.

For example, the first driving unit 700 may include a first motor 710, a ball screw bolt 720, a ball screw nut 730, and first couplers 740a and 740b.

The first motor 710 may be installed in the third coupling member 840c of the second couplers 840a, 840b, and 840c of the second driving unit 800. Accordingly, the height of the first motor 710 may be changed by the second driving unit 800.

The rotation axis of the first motor 710 may be coupled to the ball screw bolt 720.

The ball screw bolt 720 may be combined with the ball screw nut 730.

The ball screw nut 730 may be supported by being coupled to the ball screw bolt 720 and may be coupled to the first couplers 740a and 740b.

When the ball screw bolt 720 rotates by the first motor 710, the ball screw nut 730 can be raised and lowered.

The first couplers 740a and 740b may be combined with the ball screw nut 730 and the magnetic rod holder 500.

Specifically, for example, the first couplers (740a, 740b) are a first coupling member (740a) coupled to the ball screw nut 730, a first coupling member (740a), and a first coupling member (740a) coupled to the magnetic rod holder 500. It may include two coupling members (740b).

Accordingly, the first couplers 740a and 740b can move the magnetic rod holder 500 up and down.

Meanwhile, the ball screw bolt 720, ball screw nut 730, and first coupler (740a, 740b) are directly or indirectly coupled to the first motor 710, so that the first motor 710 is connected to the second driving unit 800. When moved up and down, the entire first driving unit 700 can move up and down.

Additionally, when the first motor 710 moves up and down by the second driving part 800, the magnetic rod holder 500 coupled to the first driving part 700 can also move up and down. Accordingly, the height of the magnetic rod holder 500 is the first height determined by the second driving part 800 (e.g., the height of the cap holder 600, the first motor 710, or the first driving part 700) and the first height determined by the second driving part 800. It can be calculated by adding up the second height (relative height to the first height) determined by the first driving unit 700 based on one height.

The first couplers (740a, 740b) may be coupled to the fourth guide coupling portion (M13) that is slidably coupled to the first guide portion (GR1). Specifically, the second coupling member 740b of the first couplers 740a and 740b may be coupled to the fourth guide coupling portion M13.

Meanwhile, the magnetic rod holder 500 coupled to the first coupler (740a, 740b) can be slidably coupled not only to the first guide portion (GR1) but also to the second guide portion (GR2). For example, the magnetic rod holder 500 may be coupled to the fifth guide coupling portion M22 that is slidably coupled to the second guide portion GR2.

Accordingly, since the magnetic rod holder 500 is guided by the first and second guide parts (GR1, GR2) arranged side by side at a predetermined interval, the magnetic rod holder 500 is stably lifted and lowered without being tilted to one side. can do. In particular, when the cap holder 600 quickly vibrates up and down to perform a stirring operation, the magnetic rod holder 500 can also quickly and stably vibrate up and down together with the cap holder 600. In addition, even if the size or width of the magnetic rod holder 500 is large, the magnetic rod holder 500 can be stably raised and lowered without tilting to one side.

Meanwhile, a second sensor (S2) for setting the reference height of the magnetic rod holder 500 may be disposed in the magnetic rod holder 500, the first driving unit 700, or the second driving unit 800. The second sensor S2 may be connected to the control unit 1100.

Specifically, for example, as shown in FIGS. 44 and 48, a protrusion (P2) may be formed on the first coupling member (740a) of the first coupler (740), and a second sensor (S2) that detects the protrusion (P2) ) may be disposed on the third coupling member 840c of the second coupler 840. The first coupling member 740a of the first coupler 740 moves up and down by the first motor 710 so that the protrusion P2 is moved at a predetermined distance from the third coupling member 840c of the second coupler 840. When located at (height), the second sensor (S2) can detect the protrusion (P2) and transmit the detected information to the control unit 1100, and the control unit 1100 sets the reference position for controlling the first motor 710. (Reference height) can be set.

In this way, the second driving unit 800 can move the cap holder 600 and the first driving unit 700 up and down together.

Accordingly, when the second driving part 800 moves the cap holder 600 up and down, the magnetic rod holder 500 coupled to the first driving part 700 can also move up and down together. Accordingly, when the height of the cap holder 600 changes, the height of the magnetic rod holder 500 may also change equally. Accordingly, the cap holder 600 and the magnetic rod holder 500 can be controlled more simply and stably than when they move up and down independently of each other, and malfunctions such as misjudging the positions (heights) of the holders 500 and 600 can be prevented. It can prevent damage to the nucleic acid extraction device.

Specifically, the cap holder 600 rises together with the magnetic rod holder 500, so when the cap holder 600 is raised, the cap holder 600 or the cap (C) moves with the magnetic rod holder 500 or the magnetic rod 400. ) There is no need to control it to avoid colliding with it. In addition, since the cap holder 600 goes up and down together with the magnetic rod holder 500, the magnetic rod holder 500 always moves up and down the cap holder 600 by a predetermined distance (height). It can be located at the top. Accordingly, since the magnetic rod holder 500 can stably descend by the predetermined distance (height), there is no need to consider the position (height) of the cap holder 600 when lowering the magnetic rod holder 500.

On the other hand, in the related art, in order to prevent damage or malfunction of the nucleic acid extraction device due to collision between the cap holder 600 or cap C and the magnetic rod holder 500 or magnetic rod 400, the holders 500 and 600 The current position (height) was determined/detected and the holders (500, 600) were moved based on this. Accordingly, control of the nucleic acid extraction device becomes complicated, unexpected errors may occur, and if the current position (height) of the holders 500, 600 is incorrectly determined due to a malfunction of the sensor, etc., there is a problem of damage to the nucleic acid extraction device.

Additionally, the magnetic rod holder 500, the cap holder 600, and the first driving unit 700 may be slidably coupled to the same first guide unit GR1 extending up and down.

Accordingly, the magnetic rod holder 500, the cap holder 600, and the first driving unit 700 can be moved by being guided by the same first guide unit GR1. Accordingly, the magnetic rod holder 500 and the cap holder 600 are relatively tilted toward each other to prevent the magnetic rod 400 from being inserted into the cap C or from damaging or pressing the cap C. In addition, damage to the nucleic acid extraction device can be prevented by the magnetic rod holder 500 and the first driving unit 700 being relatively tilted toward each other. In particular, the magnetic rod holder 500, cap holder 600, and first driving unit 700 are guided to the same first guide unit GR1, so that even if vibration is generated by the driving units 700, 800, and 900, the nucleic acid extraction device can operate stably.

Figure 49 is a diagram showing the operating states of the cap holder, magnetic rod, and magnetic rod holder in the nucleic acid extraction device according to an embodiment of the present invention.

Referring to FIG. 49, in the process of extracting nucleic acid using the nucleic acid extraction device 10, when the magnetic rod holder 500 rises from the cap holder 600 to its maximum height, the magnetic rod holder 500 coupled to the magnetic rod holder 500 The magnetic rod 400 may be inserted into the third through hole 610 formed in the cap holder 600 and communicating with the inside of the cap C coupled to the cap holder 600.

However, it is not limited to this configuration. For example, unlike FIG. 49, in the process of extracting nucleic acid using the nucleic acid extraction device 10, when the magnetic rod holder 500 rises from the cap holder 600 to its maximum height, the magnetic rod holder 500 The magnetic rod 400 coupled to may be inserted into the cap C coupled to the cap holder 500.

Since the cap holder 600 and the magnetic rod holder 500 move up and down together, the end of the magnetic rod 400 can always remain inserted into the third through hole 610 or the cap C. Accordingly, it is possible to prevent the magnetic rod 400 from being inserted into the cap C due to vibration or the like. Accordingly, nucleic acid extraction can be performed easily and stably and damage to the nucleic acid extraction device can be prevented.

[3rd District East]

Figures 50 and 51 are perspective views showing main components related to the third driving unit according to an embodiment of the present invention. Figure 52 is an exploded perspective view of Figure 51.

The third driving unit 900 may be installed on the base 100.

The third driving unit 900 may move the magnetic rod holder 500 and the cap holder 600 in the first direction or the slot 200 in the first direction.

Accordingly, the third driving unit 900 moves the cap C coupled to the cap holder 600 and the magnetic rod 400 coupled to the magnetic rod holder 500 to another well W in the first direction, for example, It can be placed at the top of the well (W) of the row.

Specifically, for example, the third driving unit 900 includes a third motor 910, a second driving pulley (920a), a second driven pulley (920b), a second belt 930, and a third coupler (940a, 940b). ) may include.

The third motor 910 may be installed in the base 100. The rotation axis of the third motor 910 may be coupled to the second driving pulley 920a.

The second driving pulley 920a and the second driven pulley 920b may be installed on the base 100 in a first direction and may be connected to each other through a second belt 930.

The second belt 930 may have irregularities formed on one surface facing the pulleys 920a and 920b and may be coupled to the third coupler 940.

When the second driving pulley (920a) is rotated by the third motor (910), the unevenness of the second belt (930) between the second driving pulley (920a) and the second driven pulley (920b) can move in the first direction. there is.

The third couplers 940a and 940b may be combined with the second belt 930 and the main frame 300.

Specifically, for example, the third couplers (940a, 940b) are in contact with one surface of the second belt 930 on which irregularities are formed, and the first coupling member ( 940a) and a second coupling member 940b that is in contact with the other surface of the second belt 930, is coupled to the first coupling member 940a, and is coupled to the main frame 300.

Accordingly, the third couplers 940a and 940b can move the main frame 300 in the first direction.

Meanwhile, the bottom frame 310 may be coupled to the sixth guide coupling portion M3 that is slidably coupled to the third and fourth guide portions GR3 and GR4.

A third sensor S3 may be disposed on the base 100, the main frame 300, or the third driving unit 900 to set the reference position of the main frame 300 in the first direction. The third sensor S3 may be connected to the control unit 1100.

Specifically, for example, as shown in FIGS. 51 and 52, a protrusion (P3) may be formed on the bottom frame 310 of the main frame 300, and a third sensor (S3) that detects the protrusion (P3) may be formed on the base. It can be placed at a predetermined location (100). When the floor frame 310 moves in the first direction by the third motor 910 and the protrusion P3 is located at the predetermined position, the third sensor S3 detects the protrusion P3 and the control unit 1100 ), and the control unit 1100 can set a reference position for controlling the third motor 910.

[Power supply]

The power supply unit 1000 may supply power to the first, second, and third driving units 700, 800, and 900, the control unit 1100, and the display unit 1400.

[Control unit]

The control unit 1100 can control the first/second/third driving units 700, 800, and 900 and can control the temperature of the heater 220 of the slot 200.

In addition, the control unit 800 operates the first/second/third driving units 700, 800, and 900 for each of a plurality of stages in a given order (e.g., lysis stage, first cleaning stage, second cleaning stage, and illusion stage). Control information or control information of the temperature of the heater 220 can be stored.

The control unit 800 may control the first/second/third driving units 700, 800, and 900 or control the temperature of the heater 220 based on the control information.

[housing]

The housing 1200 can have the appearance of a nucleic acid extraction device and can accommodate the first/second/third driving parts (700, 800, 900), etc. inside, and the first/second/third driving parts (700) , 800, 900) can be protected.

[door]

The door 1300 can be formed in the housing 1200 and can be opened and closed. When the door 1300 is opened, the slot 200 can be pulled out and the adapter 30 can be inserted into the cartridge 20.

[Display part]

The display unit 1400 may display the currently performed step among the plurality of steps.

[Input section]

The input unit 1500 may be a touch screen device provided together with the display unit 1400.

The input unit 1500 may receive control information of the first, second, and third driving units 700, 800, and 900 or control information of the temperature of the heater 220 from the user.

[Ministry of Communications]

The communication unit 1600 (FIG. 1) may provide an input/output interface to the user. For example, the communication unit 1600 may include a port for serial communication (eg, RS-232).

Through the communication unit 1600, the user can send control information of the driving unit 200 or control information of the temperature of the heating element 500 to the control unit for each of a plurality of steps in a given order (e.g., binding step, cleaning step, drying step, illusion step). It can be saved at (800).

As described above, the present invention has been described with reference to the illustrative drawings, but the present invention is not limited to the embodiments and drawings disclosed herein, and various modifications may be made by those skilled in the art within the scope of the technical idea of the present invention. It is obvious that transformation can occur. In addition, although the operational effects according to the configuration of the present invention were not explicitly described and explained while explaining the embodiments of the present invention above, it is natural that the predictable effects due to the configuration should also be recognized.

10: Nucleic acid extraction device
20: Cartridge 30: Adapter
40: strip
100: Base 110: Stopper
200: slot 210: receiving groove
220: Heater 230: First ball plunger
240: stopper
300: main frame 310: floor frame
320: Central frame
400: magnetic rod 500: magnetic rod holder
600: Cap holder 610: Third through hole
620: Insertion groove 630: Second ball plunger
700: first driving unit 710: first motor
720: Ballscrew bolt 730: Ballscrew nut
740: First coupler
800: second driving unit 810: second motor
820: Pulley 830: First belt
840: Second coupler
900: Third driving unit 910: Third motor
920: Pulley 930: Second belt
940: Third coupler
1000: Power supply unit 1100: Control unit
1200: Housing 1300: Door
1400: display unit 1500: input unit
1600: Ministry of Communications

Claims (9)

A slot 200 in which a receiving groove 210 is formed into which a cartridge 20 having one or more wells (W) or an adapter 30 coupled to one or more of the cartridges 20 is inserted;
One or more magnetic rods 400;
A magnetic rod holder (500) coupled to the magnetic rod (400);
A cap holder (600) disposed below the magnetic rod holder (500) and coupled to one or more caps (C);
It is connected to the magnetic rod holder 500 and moves the magnetic rod holder 500 up and down so that the magnetic rod 400 enters the inside of the cap (C) coupled to the cap holder 600 or the cap (C) ) a first driving unit 700 that is retracted from the inside of the;
It is connected to the cap holder 600 and the first driving part 700, moves the cap holder 600 and the first driving part 700 together up and down, and places the cap C coupled to the cap holder 600 in the receiving groove. Entering the well (W) of the cartridge 20 inserted into (210) or the well (W) of the cartridge 20 combined with the adapter 30 inserted into the receiving groove 210, or entering the well (W) a second driving unit 800 that is retracted from the inside; and
It includes a third driving unit 900 that moves the magnetic rod holder 500 and the cap holder 600 in a first direction or moves the slot 200 in a first direction,
The height of the magnetic rod holder 500 is the sum of the first height determined by the second driving unit 800 and the second height, which is a relative height determined by the first driving unit 700 based on the first height. Calculated by
Nucleic acid extraction device.
In claim 1,
In the process of extracting nucleic acids using the nucleic acid extraction device 10, when the magnetic rod holder 500 rises from the cap holder 600 to its maximum height, the magnetic rod coupled to the magnetic rod holder 500 (400) is inserted into the through hole 610 formed in the cap holder 600 and communicating with the inside of the cap C coupled to the cap holder 600, or the cap coupled to the cap holder 500 ( C) Nucleic acid extraction device inserted into C).
In claim 1,
A nucleic acid extraction device in which the magnetic rod holder 500, cap holder 600, and first driving unit 700 are slidably coupled to the same first guide unit GR1 extending up and down.
In claim 1,
The second driving unit 800 includes a second motor 810 and a motion conversion means (T) that converts the rotational motion of the second motor 810 into a vertical translational motion,
The cap holder 600 extends up and down and is slidably coupled to the first guide part GR1 and the second guide part GR2 arranged side by side at a predetermined interval, respectively,
The nucleic acid extraction device wherein the motion conversion means (T) is disposed between the first guide part (GR1) and the second guide part (GR2).
In claim 1,
One or more grooves are formed on the inner surface of the receiving groove 210 in a direction crossing the direction in which the cartridge 20 or adapter 30 is inserted into the receiving groove 210,
A first ball plunger 230 is installed in each of the grooves,
The end of the first ball plunger 230 protrudes inside the receiving groove 210,
A first ball plunger groove 36 or a protrusion 24 is formed on the outer surface of the cartridge 20 or adapter 30,
When the cartridge 20 or adapter 30 is inserted into the receiving groove 210, the end of the first ball plunger 230 is inserted into the first ball plunger groove 36 or the protrusion 24 is inserted into the first ball plunger groove 36. Nucleic acid extraction device located on top of the protrusion 24.
In claim 5,
The receiving groove 210 includes an outer groove 212 that is formed on the outside and has a larger width, and an inner groove 214 that is formed on the inside of the outer groove 212 and has a smaller width,
A step S is formed on the inner surface of the receiving groove 210 by the outer groove 212 and the inner groove 214,
The first ball plunger (230) is a nucleic acid extraction device installed on the inner surface of the outer groove (212).
In claim 1,
The slot 200 slides into and out of the nucleic acid extraction device,
The nucleic acid extraction device is provided with a locking protrusion 110 that contacts the slot 200 or faces it at a predetermined distance when the slot 200 is inserted,
A nucleic acid extraction device in which the slot 200 and the locking protrusion 110 are each provided with a magnet or a magnet and a metal at a predetermined portion that contacts or faces each other.
In claim 1,
A strip 40 including one or more caps C into which the magnetic rod 400 is inserted and a connecting portion 42 extending in a direction intersecting the direction in which the caps C extend and connecting the caps C. ) and further includes,
The cap holder 600 is formed with one or more insertion grooves 620 into which the strip 40 is inserted,
A groove or through hole is formed on the inner surface of the insertion groove 620 in a direction crossing the direction in which the strip 40 is inserted into the insertion groove 620,
A second ball plunger 630 is disposed in the groove or through hole,
The end of the second ball plunger 630 protrudes inside the insertion groove 620,
A second ball plunger groove 44 is formed in the connection portion 42,
When the strip (40) is inserted into the insertion groove (620), the end of the second ball plunger (630) is inserted into the second ball plunger groove (44).
In claim 1,
The cap (C) is,
A hollow body is formed that extends up and down and is open at the top,
A nucleic acid extraction device comprising a section (R) in which the width decreases toward the bottom, but the rate of decrease in width gradually decreases.