KR102208785B1 - Microfluidic droplet generating device - Google Patents

Abstract

The present invention relates to a microfluidic droplet generating device, which can easily adjust the size of microdroplets through a combination of bolts and nuts and rotation of the bolts. The microfluidic droplet generating device according to an embodiment of the present invention includes: a bolt; a nut screwed to the bolt through an upper hole; and a body integrally formed with the nut and having two channels formed therein through which a fluid can be injected from the outside and flow, wherein a portion of a lower hole of the nut and the two channels may be connected, and the two channels may include a first channel and a second channel.

Description

Microfluidic droplet generating device}

The present invention relates to an apparatus for generating microdroplets, and more particularly, to an apparatus for generating microdroplets capable of adjusting the size of microdroplets by coupling a bolt and a nut and rotating the bolt.

Microdroplet technology is a technology field that has recently been in the spotlight due to a relatively simple preparation process, uniformity of droplets, possibility of controlling droplet size and volume, and high productivity. (Non-patent documents 1 to 3)

Microdroplets can be particularly used as a reactor in various fields such as nanoparticle synthesis, polymer synthesis, cell biology, and nucleic acid analysis. (Non-Patent Documents 4 to 11)

Techniques for manufacturing devices for generating microdroplets mostly rely on lithography or soft lithography methods. However, manufacturing a microdroplet generating device using a lithography method has disadvantages of having to go through a high cost, a long manufacturing process, and a layer-by-layer bonding process.

As an alternative to this problem, in a method of manufacturing a device capable of generating microdroplets, a method of manufacturing a microdroplet generating device using a 3D printer technology, which can be manufactured at a faster and lower cost than a lithography method, has emerged. (Non-Patent Documents 12 and 13)

S. Mashaghi, A. Abbaspourrad, D.A. Weitz, A.M. van Oijen, Droplet microfluidics: A tool for biology, chemistry and nanotechnology, Trends Analyt. Chem., 82 (2016) 118-25. T.S. Kaminski, P. Garstecki, Controlled droplet microfluidic systems for multistep chemical and biological assays, Chem. Soc. Rev., 46 (2017) 6210-26. Y. Zhang, B. Zhu, Y. Liu, G. Wittstock, Hydrodynamic dispensing and electrical manipulation of attolitre droplets, Nat. Commun., 7 (2016) 12424. Q. Ji, J.M. Zhang, Y. Liu, X. Li, P. Lv, D. Jin, H. Duan, A Modular Microfluidic Device via Multimaterial 3D Printing for Emulsion Generation, Sci. Rep., 8 (2018) 4791. A. Toor, S. Lamb, B.A. Helms, T.P. Russell, Reconfigurable Microfluidic Droplets Stabilized by Nanoparticle Surfactants, ACS Nano, 12 (2018) 2365-72. D.M. Headen, J.R. Garcia, A.J. Garcia, Parallel droplet microfluidics for high throughput cell encapsulation and synthetic microgel generation, Microsyst. Nanoeng., 4 (2018) 17076. S. Yadavali, H.H. Jeong, D. Lee, D. Issadore, Silicon and glass very large scale microfluidic droplet integration for terascale generation of polymer microparticles, Nat. Commun., 9 (2018) 1222. B.L. Wang, A. Ghaderi, H. Zhou, J. Agresti, D.A. Weitz, G.R. Fink, G. Stephanopoulos, Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption, Nat. Biotechnol., 32 (2014) 473. X. Zhao, S. Liu, L. Yildirimer, H. Zhao, R. Ding, H. Wang, W. Cui, D. Weitz, Injectable Stem Cell-Laden Photocrosslinkable Microspheres Fabricated Using Microfluidics for Rapid Generation of Osteogenic Tissue Constructs, Adv. Funct. Mater., 26 (2016) 2809-19. W. Zhang, N. Li, D. Koga, Y. Zhang, H. Zeng, H. Nakajima, J.M. Lin, K. Uchiyama, Inkjet Printing Based Droplet Generation for Integrated Online Digital Polymerase Chain Reaction, Anal. Chem., 90 (2018) 5329-34. M. Nie, M. Zheng, C. Li, F. Shen, M. Liu, H. Luo, X. Song, Y. Lan, J.Z. Pan, W. Du, Assembled Step Emulsification Device for Multiplex Droplet Digital Polymerase Chain Reaction, Anal. Chem., 91 (2019), 1779-1784. W. Li, L. Zhang, X. Ge, B. Xu, W. Zhang, L. Qu, C.H. Choi, J. Xu, A. Zhang, H. Lee, D.A. Weitz, Microfluidic fabrication of microparticles for biomedical applications, Chem. Soc. Rev., 47 (2018) 5646-83. C.M. Ho, S.H. Ng, K.H.H. Li, Y.J. Yoon, 3D printed microfluidics for biological applications, Lab Chip, 15 (2015) 3627-37.

An object of the present invention is to solve the above-described problem, and an object of the present invention is to provide an apparatus for generating microscopic droplets that can be manufactured by a 3D printer.

In addition, it is an object of the present invention to provide a microdroplet generating apparatus capable of relatively accurately adjusting the size of microdroplets by a simple method of coupling a bolt and a nut and rotating the bolt.

The objects of the present invention are not limited to the above-described objects, and other objects that are not mentioned will be clearly understood from the following description.

The microdrop generating apparatus according to an embodiment of the present invention includes a bolt; A nut screwed to the bolt through an upper hole; And a body integrally formed with the nut and having two channels formed therein through which fluid is injected from the outside to flow, and a lower hole of the nut and the two channels are partially connected, and the two channels May include a first channel and a second channel.

The first channel may include an inlet and an outlet, and the second channel may include an inlet provided below the inlet of the first channel and an outlet connected to the first channel.

The first channel and the second channel may extend parallel to each other from each inlet, and an outlet of the second channel may be vertically connected to a portion of the first channel.

The first channel is connected to the lower hole of the nut, and the outlet of the second channel is provided inside the virtually extended cross section when the cross section of the lower hole is virtually extended in the axial direction of the nut. Can be.

The first channel may be for flow of an oil phase fluid, and the second channel may be for flow of a water phase fluid.

At a junction between the first channel and the outlet of the second channel, the oily fluid flowing in the first channel and the aqueous fluid output from the outlet of the second channel may meet to generate microdroplets.

The rotation angle of the bolt coupled with the nut and the distance between the lower surface of the bolt coupled with the nut and the outlet of the second channel may have a relationship as shown in the following equation.

Here, hg is the distance between the lower surface of the bolt and the outlet of the second channel, p is the pitch, which is the distance between the thread and the thread, and α is the rotation angle of the bolt.

The size of the generated microdroplet may be dependent on a distance between the lower surface of the bolt coupled with the nut and the outlet of the second channel.

When the flow rate of the fluid is higher than a predetermined speed, the size of the generated microdroplets and the distance between the lower surface of the bolt coupled to the nut and the outlet of the second channel may have a correlation.

The microdroplet generating apparatus includes flow paths respectively coupled to inlets of the first channel and the second channel, the flow paths are each coupled to different fluid sources, and the fluid sources are the same syringe pump (syringe pump). pump).

The flow rate of the fluid injected into the first channel and the second channel may be varied according to the pumping speed of the syringe pump.

The microdroplet generating apparatus may be manufactured by a 3D printer.

Since the microdroplet generating device according to an embodiment of the present invention is manufactured through a 3D printer, the cost is reduced compared to the production of the microdroplet generating device using a method such as lithography, and the manufacturing time is drastically reduced. There is an effect that you can.

In addition, since the microdroplet generating apparatus according to an embodiment of the present invention can adjust the size of the microdroplet through rotation of a bolt, there is an effect that the size of the microdroplet can be accurately controlled by a simple method.

In addition, the microdroplet generating apparatus according to an embodiment of the present invention has an effect that, unlike the conventional microdroplet generating apparatus, the size of the microdroplets can be adjusted using only one syringe pump.

The effects of the present invention are not limited to the effects mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the following description.

1 is a diagram showing an internal configuration of a microdroplet generating apparatus.
2 is a cross-sectional view of an apparatus for generating microdroplets.
3 is a diagram showing a fluid supply source and a syringe pump for injecting a fluid into a microdroplet generating device.
FIG. 4 is a view showing an image of a microdroplet generating device that adjusts the size of a microdroplet through rotation of a bolt and a microscope image of the microdroplet generated according to the width of the drop generator.
5 is a view viewed from above that the bolt is coupled to the nut, and it shows that teeth are formed in the bolt so that the angle can be accurately adjusted.
6 is a microscope image showing the size of a microdroplet that varies according to the flow rate of a fluid by maintaining a constant width of the droplet generating unit and controlling the pumping speed of a syringe pump, and a graph showing the relationship between the width of the droplet generating unit and the size of the microdroplet. .
7 is an image and graph showing the size of poly(ethylene glycol) diacrylate (PEGDA) particles according to the width of a droplet generating portion in a state where a flow rate of 150 μL/min is kept constant.

Specific structural or functional descriptions of the embodiments of the present invention disclosed in this specification or application are exemplified only for the purpose of describing the embodiments according to the present invention, and the embodiments according to the present invention may be implemented in various forms. And should not be construed as limited to the embodiments described in this specification or application.

Since the embodiments according to the present invention can be modified in various ways and have various forms, specific embodiments will be illustrated in the drawings and described in detail in the present specification or application. However, this is not intended to limit the embodiments according to the concept of the present invention to a specific form of disclosure, and it should be understood that all changes, equivalents, and substitutes included in the spirit and scope of the present invention are included.

In the present specification, terms such as first and/or second are used only for the purpose of distinguishing one component from another component. That is, it is not intended to limit the components by the terms.

Components, features, and steps referred to as'include' in this specification mean the existence of the corresponding components, features, and steps, and are intended to exclude one or more other components, features, steps, and equivalents thereof. This is not.

Unless otherwise specified and stated in the singular form in the specification, plural forms are included. That is, the components and the like mentioned in the present specification may mean the presence or addition of one or more other components.

Unless otherwise defined, all terms used in this specification, including technical or scientific terms, have the same meaning as commonly understood by a person of ordinary skill in the art to which the present invention belongs. to be.

That is, terms such as those defined in commonly used dictionaries should be interpreted as having meanings consistent with the meaning of the context of the related technology, and should be interpreted as ideal or excessively formal meanings unless explicitly defined in this specification. It doesn't work.

Hereinafter, the present invention will be described in detail by describing a preferred embodiment of the present invention with reference to the accompanying drawings.

1 is a diagram showing an internal configuration of a microdroplet generating apparatus.

Referring to FIG. 1, a microdrop generating apparatus according to an embodiment of the present invention includes a bolt 20, a nut 110 screwed to the bolt 20, and a body 10 integrally formed with the nut 110. It may include. The body 10 may include a first channel 120 and a second channel 130 which are two channels through which a fluid is injected and flowed from the outside while being integral with the nut 110.

The bolt 20 is a component of the microdroplet generating apparatus according to an embodiment of the present invention, and the nut 110 and the screw through the upper hole 111 of the nut 110 integral with the body 10 Are combined. The degree of protrusion of the lower portion of the bolt is adjusted through the rotation of the bolt 20, and the size of the microdroplet to be generated is adjusted as the distance between the lower portion of the bolt and the outlet of the second channel 130 is adjusted. A mechanism by which the size of the microdroplet is adjusted through the rotation of the bolt 20 will be described later.

The body 10 is a component of the microdrop generating apparatus according to an embodiment of the present invention, and is integrally formed with the nut 110, which is another component, and a first channel 120 and a second channel formed therein. It may include a channel 130.

The nut 110 is a place where a female thread is formed on its inner surface and is screwed with the bolt 20. The nut 110 may be integrally formed with the body 10 of the microdrop generating device and may be located above the body 10.

The body 10 may include a first channel 120 through which an oil phase fluid flows and a second channel 130 through which a water phase fluid flows.

The lower hole 112 of the nut 110 formed integrally with the body 10, and a portion of the first channel 120 and the second channel 130 are connected to each other, and microdroplets may be generated at this junction. .

Specifically, the first channel 120 includes an inlet and an outlet, and may extend from the inlet in a horizontal direction to form an outlet. The inlet of the second channel 130 is provided below the inlet of the first channel 120, and the outlet of the second channel 130 may be connected to the first channel 120. The second channel 130 may extend parallel to the first channel 120 from the inlet, and the outlet of the second channel 130 may be connected vertically to a portion of the first channel 120. That is, the second channel 130 is formed to extend in parallel with the first channel 120 in a horizontal direction, and the lower hole 112 is connected to the first channel 120 and the second channel 130. From the side, it is bent vertically in the direction of the first channel 120 so that the fluid outflow direction of the outlet of the second channel 130 is orthogonal to the direction in which the first channel 120 extends.

When the lower hole 112 of the nut 110 formed integrally with the body 10 is connected to the first channel 120 and the cross section of the lower hole 112 is virtually extended in the axial direction of the nut 110 , It may have a connection structure in which an outlet of the second channel 130 is provided inside the virtually extended virtual cross section.

That is, the inside of the nut 110 is formed in a vertical direction, and the lower hole 112 of the nut 110 is connected to the first channel 120. The cross section of the lower hole 112 may be formed to be spaced apart from the upper portion of the outlet of the second channel 130.

Referring to FIG. 2, the connection structure of the nut 110, the first channel 120 and the second channel 130 and the principle of adjusting the size of the microdroplet through the bolt 20 can be confirmed.

An oily fluid may be injected into the first channel 120 to flow in a horizontal direction, and the aqueous fluid may flow through the second channel 130 to be injected into the first channel 120 in a vertical direction. At the junction of the outlet of the first channel 120 and the second channel 130, an interface between the two phases is created due to the encounter of two fluids having different phases, and the fluid is continuously injected, and a microfluid of uniform size Enemies can be formed.

As described above, the lower hole 112 of the nut 110 is connected to the first channel 120, and when the cross section of the lower hole 112 is virtually extended in the axial direction of the nut 110, the inside of the virtually extended cross-section is The outlet of the second channel 130 is included. That is, the lower hole 112 of the nut 110 is formed above the portion where the first channel 120 and the second channel 130 are vertically connected.

Accordingly, when the bolt 20 is screwed to the nut 110, microdroplets may be generated in a region between the lower surface of the bolt 20 and the outlet of the second channel 130. Hereinafter, the area between the lower surface of the bolt 20 and the outlet of the second channel 130 will be referred to as the droplet generating unit 140.

According to the connection structure between the channels as described above, the aqueous fluid is injected in the vertical direction into the first channel 120 and the width of the droplet generating unit 140 can be adjusted by the position of the lower surface of the nut 110, The size of the microdroplet may be adjusted through the rotation of the bolt 20.

3 is an image showing the injection of fluid through one syringe pump into the microdrop generating apparatus according to an embodiment of the present invention.

Referring to FIG. 3, a flow path is coupled to the inlet of the first channel 120 and the second channel 130, and the flow paths are respectively coupled to different fluid supply sources including oily fluid and aqueous fluid, Different fluid sources are installed on the same syringe pump. A fluid may be injected into the first channel 120 and the second channel 130 through pumping of a syringe pump. An oily fluid may be injected into the first channel 120, and an aqueous fluid may be injected into the second channel 130.

Conventional microdroplet generating apparatus required two or more syringe pumps to control the size of microdroplets, but the microdroplet generating apparatus according to an embodiment of the present invention prevents a bolt-nut combination and rotation of the bolt. By using the bar, the size of the microdroplet can be adjusted, and the size of the microdroplet can be adjusted with only one syringe pump.

The flow rate of the fluid injected into the first channel 120 and the second channel 130 may be adjusted through the syringe pump. Since different fluid supply sources are installed in one syringe pump, the flow rates of the fluid entering the first channel 120 and the second channel 130 may be set equally.

By adjusting the pumping speed of the syringe pump to control the flow rate of the fluid, the size of the microdroplet can be controlled. That is, the flow rate of the fluid injected into the first channel 120 and the second channel 130 is variable according to the pumping speed of the syringe pump. The principle of controlling the size of the microdroplet according to the flow rate of the fluid will be described later.

FIG. 4 is a view showing an image of a microdroplet generating device for adjusting the size of a microdroplet through rotation of a bolt and a microscopic image of microdroplets according to the width of the drop generator.

Referring to FIG. 4, it can be seen that the size of the microdroplet is adjusted by adjusting the width of the droplet generating unit 140 through rotation of the bolt 20.

The bolt 20 is coupled to the nut 110 forming a part of the body 10, and the rotational motion of the bolt 20 is converted into a vertical motion, so that the width of the droplet generating unit 140 is adjusted and through this The size of the microdroplets can be adjusted. That is, the size of the generated microdroplet may depend on the distance between the lower surface of the bolt 20 coupled with the nut 110 and the outlet of the second channel 130.

The width of the droplet generating unit 140 may be accurately calculated through the rotation angle of the bolt 20 as shown in Equation 1 below.

hg is the width of the droplet generating unit 140, p is the pitch, which is the distance between the thread and the thread, and α is the rotation angle of the bolt 20. For example, if the distance between the thread and the thread is 0.75 mm, and the bolt 20 is rotated by 4.8 degrees, hg becomes 10 μm.

When the bolt 20 is completely tightened to the nut 110 forming a part of the body 10, the lower surface of the bolt 20 may be designed to contact the outlet of the second channel 130. In this case, hg = 0 and the outlet of the second channel 130 is blocked by the lower surface of the bolt 20, so that the aqueous fluid cannot be injected into the droplet generating unit 140, so that micro droplets can be generated. none.

It is possible to adjust the size of the microdroplet by calculating the rotation angle of the bolt 20 by holding the point at hg=0 as a reference. If the flow rate of the fluid is kept constant, the size of the microdroplets increases as hg increases.

Accurately adjusting the width of the droplet generating unit 140 is a very important factor in controlling the size of the microdroplets, so it should be possible to accurately control the rotation angle of the bolt 20. To this end, it is possible to control the rotation angle by forming a tooth (teeth) on the upper end of the bolt (20).

5 is a view as viewed from above that the bolt is coupled to a nut forming a part of the body.

Referring to FIG. 5, it can be seen that the bolt 20 has teeth formed therein, and the rotation angle of the bolt 20 can be accurately controlled.

For example, when 75 teeth are generated in a bolt, the rotation angle when rotating one tooth is 360/75=4.8 degrees. Therefore, since the rotation angle can be calculated through the number of rotated teeth, the size of the microdroplets can be easily adjusted manually.

The size of the microdroplets may be adjusted not only by the width of the droplet generating unit 140 but also by the flow rates of the aqueous fluid and the oily fluid. Therefore, by controlling the rotation of the bolt 20 and the flow rate of the solution together, microdroplets of various sizes can be manufactured.

In the initial generation stage of the microdroplet, as the aqueous fluid is injected into the oily fluid flowing in the horizontal direction, the droplet is generated in the form of a plug, and the droplet at the initial stage is more than hg (the width of the droplet generating unit 140). It has a large size. After the droplets are generated in the initial stage, when the viscous force becomes greater than the interfacial tension, the stopper-shaped droplet is differentiated into droplet-shaped micro droplets.

However, when the flow velocity of the fluid is not sufficient, sufficient shear stress is not applied to the droplet, and the interfacial tension acts as a factor that causes the droplet to grow regardless of hg (width of the droplet generating unit 140). Therefore, regardless of the size of hg (the width of the droplet generating unit 140), microdroplets having a similar size are generated.

On the other hand, when the flow velocity of the fluid is sufficiently fast, it can be seen that the size of the microdroplet is linearly proportional to hg (the width of the droplet generating unit 140), although there is a slight error. That is, the size of the microdroplet and hg may have a correlation.

With reference to the drawings, the relationship between hg (width of the droplet generating unit 140), the flow velocity of the fluid, and the size of the generated microdroplets will be described in more detail through the following experimental examples.

Experimental example One

An experiment was conducted to confirm the relationship between the flow velocity of the fluid and the width (hg) of the droplet generating portion and the size of the generated microdroplets. The width (hg) of the droplet generating section is set to 20 μm, 60 μm, 100 μm, and 200 μm, and the flow velocity of the fluid is set to 50 μL/min, 100 μL/min, 150 μL/min, 200 μL/min, and the respective flow velocity and the width of the droplet generating section (hg ) The size of the microdroplets generated under the condition was measured.

6 is a microscope image showing the size of the microdroplets generated under the above experimental conditions and a graph showing the relationship between the width (hg) of the droplet generation unit and the size of the microdroplets under a constant flow rate condition.

Referring to the micrograph of FIG. 6, when the flow velocity of the fluid is 100, 150, 200 μL/min, that is, when the flow velocity has a sufficient velocity, the larger the width (hg) of the droplet generating portion, the larger the size of the micro droplet. I can confirm. For example, when the flow rate of the fluid is set to 150 μL/min, when the width (hg) of the droplet generating portion is adjusted to 20, 60, 100 and 200 μm, the diameters of the microdroplets were found to be 238, 292, 547 and 952 μm, respectively.

However, when the flow rate is 50 μL/min, that is, when the flow rate of the fluid is insufficient, the size of the microdroplet is formed in the vicinity of the diameter of 1350 μm regardless of the width (hg) of the droplet generating part, and the difference is not large. I can confirm.

Referring to the graph of FIG. 6, when the flow velocity has a sufficient velocity, it can be seen that the width (hg) of the droplet generating unit and the size of the micro droplet have a slight error, but are linearly proportional.

Accordingly, microdroplets of various sizes ranging from several tens of μm to several thousands of μm can be manufactured by adjusting the width (hg) of the droplet generating unit and the flow rate of the fluid using a syringe pump together.

Experimental example 2

In a state where the flow rate of the fluid was kept constant at 150 μL/min, PEGDA (poly(ethylene glycol) diacrylate) particles were produced while adjusting the width of the droplet generating portion to 20, 60, 100 and 200 μm.

FIG. 7 is an image showing the PEGDA particles generated according to the width of the droplet generating unit while the fluid flow rate is kept constant at 150 μL/min, and a graph showing the relationship between the width of the droplet generating unit and the generated PEGDA particle size.

In this experiment, it can be confirmed through a graph that the relationship between the width of the droplet generating unit and the size of the microdroplet has a slight error but is linearly proportional.

The coefficient of variation (CV%, coefficient of variation %), which is the value obtained by dividing the standard deviation of the microdroplet size by the average value of the microdroplet size, is measured as 2.5~4.1%, and the size of the produced PEGDA particles has excellent uniformity. Was confirmed.

Through the experiment, it is possible to confirm the accuracy and uniformity of the size of the microdroplets generated by the microdroplet generating apparatus according to an embodiment of the present invention.

The apparatus for generating microdroplets according to an embodiment of the present invention may be manufactured by a 3D printer. There is no particular limitation on the types of 3D printers that can be used, but it is desirable to make a microdroplet generator with a DLP (Digital Light Processing) 3D printer in that it can shorten the manufacturing time and smooth the surface of the microdroplet generator. .

The device for generating microdroplets according to an embodiment of the present invention is a device capable of generating microdroplets by adjusting the size of microdroplets with a relatively simple structure of bolt-nut coupling, and thus, manufacturing through a 3D printer is possible without going through a complicated process. It is possible.

Through the 3D printer, the diameter of the channel, the connection structure between the channels, the pitch, and the number of teeth at the top of the bolt 20 can be variously designed according to the user's desire, and the design conditions can be entered into the 3D printer to Can manufacture enemy spawning devices.

The above description is merely illustrative of the technical idea of the present invention, and those of ordinary skill in the art to which the present invention pertains will be able to make various modifications and variations without departing from the essential characteristics of the present invention. Accordingly, the embodiments disclosed in the present invention are not intended to limit the technical idea of the present invention, but to explain the technical idea, and the scope of the technical idea of the present invention is not limited by these embodiments. The scope of protection of the present invention should be interpreted by the following claims, and all technical ideas within the scope equivalent thereto should be interpreted as being included in the scope of the present invention.

10: body
20: bolt
110: nut
111: upper hole
112: lower hole
120: first channel
130: second channel
140: droplet generation unit

Claims (12)

volt;
A nut screwed to the bolt through an upper hole; And
It comprises a body integrally formed with the nut and formed therein with two channels through which a fluid is injected and flowed from the outside,
The lower hole of the nut and the two channels are partially connected,
The two channels include a first channel and a second channel,
The first channel includes an inlet and an outlet,
The second channel includes an inlet provided below the inlet of the first channel and an outlet connected to the first channel,
The rotation angle of the bolt coupled with the nut and the distance between the lower surface of the bolt coupled with the nut and the outlet of the second channel have a relationship as shown in the following equation.

Here, hg is the distance between the lower surface of the bolt and the outlet of the second channel, p is the pitch, which is the distance between the thread and the thread, and α is the rotation angle of the bolt.
delete The method of claim 1,
The first channel and the second channel extend parallel to each other from each inlet,
The outlet of the second channel is vertically connected to a portion of the first channel,
Microdroplet generating device. The method of claim 1,
The first channel is connected to the lower hole of the nut,
The outlet of the second channel,
When the cross-section of the lower hole is virtually extended in the axial direction of the nut, it is provided inside the virtually extended virtual cross-section,
Microdroplet generating device. The method of claim 1,
The first channel is for flow of an oil phase fluid,
The second channel is for flow of a water phase fluid,
Microdroplet generating device. The method of claim 1,
At a junction between the first channel and the outlet of the second channel, the oily fluid flowing in the first channel and the aqueous fluid output from the outlet of the second channel meet to generate microdroplets,
Microdroplet generating device. delete The method of claim 6,
The size of the generated microdroplets is dependent on the distance between the lower surface of the bolt coupled with the nut and the outlet of the second channel,
Microdroplet generating device. The method of claim 6,
When the flow rate of the fluid is above a certain speed,
The size of the generated microdroplets and the distance between the lower surface of the bolt coupled with the nut and the outlet of the second channel have a correlation,
Microdroplet generating device. The method of claim 1,
The microdroplet generating device,
And flow paths respectively coupled to inlets of the first channel and the second channel,
The flow paths are each coupled to different fluid sources,
The fluid sources are installed in the same syringe pump,
Microdroplet generating device. The method of claim 10,
The flow rate of the fluid injected into the first channel and the second channel is varied according to the pumping speed of the syringe pump,
Microdroplet generating device. The method of claim 1,
The microdroplet generating device,
Manufactured by a 3D printer,
Microdroplet generating device.

Images