KR102087237B1 - Nanocomposite Hydrogel Adhered to Concrete Material for Culturing of Marine Organism - Google Patents

Abstract

The present invention relates to a nanocomposite hydrogel attached to concrete for propagating marine organisms and a method for propagating marine organisms using the same, and specifically, laponite XLG (Laponite XLG) attached to concrete for propagation of marine plants such as microalgae. N / N'-dimethyl acrylamide (hereinafter referred to as 'DMAAm') based organic / inorganic nanocomposite type hydrogels and the growth method of marine plants such as microalgae using the same) .

Description

Nanocomposite Hydrogel Adhered to Concrete Material for Culturing of Marine Organism

The present invention relates to a nanocomposite hydrogel attached to concrete for propagating marine organisms and a method for propagating marine organisms using the same, and specifically, laponite XLG (Laponite XLG) attached to concrete for propagation of marine plants such as microalgae. N / N'-dimethyl acrylamide (hereinafter referred to as 'DMAAm') based organic / inorganic nanocomposite type hydrogels and the growth method of marine plants such as microalgae using the same) .

Hydrogel is a polymer of three-dimensional network structure containing water as a solvent. Hydrogels also have the advantages of biocompatibility, high hygroscopicity, low friction, and stimulation response, but have the disadvantage of weak mechanical properties due to uneven crosslinking points. In order to improve this point, hydrogels of various concepts such as nanocomposite hydrogel, interpenetrating hydrogel, double network hydrogel, sliding hydrogel, tetra-PEG hydrogel, and ion crosslinked copolymer polymer were prepared. See Patent Document 1).

The nanocomposite hydrogel prepared by mixing with an inorganic substance is a hydrogel prepared using an inorganic clay in the form of a nano-sized disc having a negative charge instead of an organic crosslinking agent (see Non-Patent Document 2). In the nanocomposite hydrogel, the inorganic clay is stripped and uniformly dispersed in the solution state, so that the intermolecular distance between the internal crosslinking length and the surrounding clay-clay is maintained at an equal length. Hydrogels prepared using organic crosslinking agents are easily broken because the crosslinking points are fixed by chemical covalent bonds.However, nanocomposite hydrogels have excellent physical properties such as excellent elongation and elasticity because plate-like clay can move in a fluid manner. (See Non-Patent Document 2).

Nanocomposite hydrogel has a chemical structure similar to bioactive glass and is biodegradable due to neutral non-toxic substances such as Na ⁺ , Si ₄ ⁺ , Mg ₂ ⁺ , Li ⁺ . It may also be used (see Non-Patent Documents 3, 4). In addition, nanocomposite hydrogels prepared by substituting a polymerization initiator with metal oxides increase physical properties and have special advantages such as photodegradability (see Non-Patent Document 5).

Research on nanocomposite hydrogels has been focused on physical properties, stimulus response, etc., so there is not much literature on stickiness. Basically, however, nanocomposite hydrogels can be used as biocompatible adhesive materials, and biomedical applications such as denture adhesives (see Non-Patent Document 6) and wound covers (see Non-Patent Document 7) are also in progress. In the process.

Recently, some papers on the adhesion of nanocomposite hydrogels have been reported (see Non-Patent Documents 8 and 9), but there are not many studies on the attachment of marine organisms. Research on anti-fouling properties of marine organisms of a certain size, such as barnacles and cyprids, has been conducted (see Non-Patent Documents 10 and 11). There is insufficient research on the adhesion of. In particular, research on the adhesion of marine organisms is focused on the prevention of adhesion.

Various studies have also been made on the effect of titanium dioxide (TiO ₂ ) on antifouling properties (see Non-Patent Documents 12 and 13). TiO ₂ is used to prepare nanocomposite hydrogel, and since it has the advantage of increasing physical properties, TiO ₂ is used to prepare nanocomposite hydrogel and checks adhesion to materials and adhesion of microalgae. Basic research may be underway to identify the mechanism of attachment of algae.

Global catches have been stagnant since the 1980s, but global consumption of fish is steadily increasing. The consumption increase of such aquatic products is the largest by coastal growth. Fish and seaweeds account for the largest portion of coastal growth, and seaweed growth has increased the most over the last two decades (see Non-Patent Document 14).

Recently, in the coastal ecosystem, the deforestation of coastal marine forests due to the decrease of algae is rapidly progressing, which is a very big problem. Decreased marine forests lead to a disruption of coastal ecosystems, resulting in a sharp decline in coastal fisheries productivity. Accordingly, restoration of coastal ecosystems and increased productivity have become global issues.

Many countries, including Korea, are putting in artificial reefs to restore marine ecosystems and increase productivity in the near sea. However, the speed and extent to which marine plants adhere to concrete used as the material of artificial reefs is still insufficient. In order to solve these problems, attempts have been made to add inorganic components such as iron and calcium to concrete, but there are still many issues that need to be improved in marine marine propagation environment composition such as attachment and persistence.

Charles W. Peak et al., A review on tough and sticky hydrogels, Colloid Polymer Science, 291, 2031-2047, (2013). K. Haraguchi et al., Nanocomposite Hydrogels: A Unique Organic-Inorganic Network Structure with Extraordinary Mechanical, Optical, and Swelling / De-swelling Properties, Advanced Materials, 14, 1120-1123, (2002). Y. Liu et al., Injectable Dopamine-Modified Poly (ethylene glycol) Nanocomposite Hydrogel with Enhanced Adhesive Property and Bioactivity, ACS APPLIED Materials & Interfaces, 6, 16982-16992, (2014). J. I. Dawson et al., Clay: New Opportunities for Tissue Regeneration and Biomaterial Design, Advanced Materials, 25, 4069-4086, (2013). D. Zhang et al., Semiconductor nanoparticle-based hydrogels prepared via self-initiated polymerization under sunlight even visible light, Scientific Reports, 3, srep01399, (2013). X. Guo et al., Synthesis of Biocompatible Polymeric Hydrogels with Tunable Adhesion to both Hydrophobic and Hydrophilic Surfaces, Biomacromolecules, 9, 1637-1642, (2008). J. M. Rosiak et al., Medical Applications of Radiation formed Hydrogels, Radial. Phys. Chem., 42, 903-906, (1993). M. Wang et al., Polymer Nanocomposite Hydrogels Exhibiting Both Dynamic Restructuring and Unusual Adhesive Properties, Langmuir, 29, 7087-7095, (2013). S. Skelton et al., Biomimetic adhesive containing nanocomposite hydrogels with enhanced materials properties, Soft Matter, 9, 3825, (2013). T. Murosaki et al., Antifouling activity of synthetic polymer gels against cyprids of the barnacle (Balanus amphitrite) in vitro, Biofouling, 25, 313-320, (2009). T. Murosaki et al., Antifouling properties of tough gels against barnacles in a long-term marine environment experiment, Biofouling, 25, 919-1123, (2009). B. Xia et al., Interaction of TiO2 nanoparticles with the marine microalga Nitzschia closterium: Growth inhibition, oxidative stress and internalization, Science of the Total Environment, 508, 525-533, (2015). S. B. Teli et al., Fouling Resistant Polysulfone-PANI / TiO2 Ultrafiltration Nanocomposite Membranes, Industrial & Engineering Chemistry Research, 52, 9470-9479, (2013). FAO, The State of World Fisheries and Aquaculture 2016, Contributing to food security and nutrition for all. Rome. 200 pp. (2016)

The present invention is to solve the above problems, by improving the properties of the nanocomposite hydrogel nanocomposite hydrogel to increase the adhesion of concrete (artificial reef structure) for marine life growth and growth method of marine life using the same The purpose is to provide.

The present invention for solving the above problems relates to a nanocomposite hydrogel and a growth method of marine life using the same, specifically, laponite XLG (Laponite XLG) attached to concrete for the growth of marine plants such as microalgae The present invention relates to an organic / inorganic nanocomposite type hydrogel based on N, N'-dimethyl acrylamide (hereinafter referred to as 'DMAAm') and a method of propagating marine plants such as microalgae using the same. .

The first aspect of the present invention for solving the above problems is a nanocomposite hydrogel to increase the adhesion of the artificial reef structure for the growth of marine life, the monomer of the nanocomposite hydrogel is N, N'-dimethylacryl Amide (N, N'-diemthylacrylamide; DMAAm), N-isopropylacrylamide, acrylamide, vinylpyridine, vinylpyrrolidone, glycerol methacrylate, methoxy polyethylene glycol methacrylate, 2-hydroxyethyl acrylate , But may be selected from the group consisting of vinyl alcohol, methacrylic acid and acrylic acid, but is not limited thereto. It is preferred that the monomer according to the invention is a hydrophilic monomer.

According to a particular embodiment of the invention the monomer is N, N'-dimethylacrylamide (N, N-diemthylacrylamide; DMAAm).

The crosslinking agent of the nanocomposite hydrogel is N, N'-methylenebisacrylamide (MBAA), polyethylene glycol dimethacrylate, polyethylene glycol diacrylate, ethylene glycol dimethacrylate, Be selected from the group consisting of ethylene glycol diacrylate, diethylene glycol dimethacrylate, diethylene glycol diacrylate, triethylene glycol dimethacrylate and triethylene glycol diacrylate, laponite However, depending on the type of monomer, any crosslinking agent suitable for polymerization may be used without limitation, and is not limited to the crosslinking agents.

According to a particular embodiment of the invention, the crosslinker is N, N'-methylenebisacrylamide (N, N'-methylenebisacrylamide; MBAA).

The polymerization initiator of the nanocomposite hydrogel may be a radical polymerization initiator. The radical polymerization initiator means a substance capable of forming a radical by an external stimulus to initiate a polymer polymerization reaction of a monomer, and may be classified into a photo (ultraviolet) polymerization initiator, a thermal polymerization initiator, a redox initiator, and the like. In order to achieve the object of the present invention, the radical polymerization initiator is a radical polymerization initiator soluble in water, and can be produced with any radical polymerization initiator used in the art.

The polymerization initiator is, for example, peroxodisulfate calcium, titanium dioxide, benzoin, benzoin methyl ether, benzoin ethyl ether, benzoin isopropyl ether, benzoin-n-butyl ether, benzoin isobutyl ether, Acetophenone, hydroxydimethylacetophenone, dimethylaminoacetophenone, dimethoxy-2-phenylacetophenone, 3-methylacetophenone, 2,2-dimethoxy-2-phenylacetophenone, 2,2-diethoxy-2 -Phenylacetophenone, 4-chronolocetophenone, 4,4-dimethoxyacetophenone, 2-hydroxy-2-methyl-1-phenylpropan-1-one, 4-hydroxycyclophenylketone, 1-hydride Hydroxycyclohexylphenyl ketone, 2-methyl-1- [4- (methylthio) phenyl] -2-morpholino-propane-1-one, 4- (2-hydroxyethoxy) phenyl-2- (hydroxy Oxy-2-propyl) ketone, benzophenone, p-phenylbenzophenone, 4,4-diaminobenzophenone, 4,4'-diethylaminobenzophenone, dichlorobenzophenone, anthraquinone, 2-methylanthraquinone, 2-ethyl Anthraquinone, 2-t-butylanthraquinone, 2-aminoanthraquinone, 2-methylthioxanthone, 2-ethylthioxanthone, 2-chlorothioxanthone, 2,4-dimethylthioxanthone, 2,4 Diethyl thioxanthone, benzyl dimethyl ketal, diphenyl ketone benzyl dimethyl ketal, acetophenone dimethyl ketal, p-dimethylaminobenzoic acid ester, 2,4,6-trimethylbenzoyl-diphenyl-phosphine oxide, fluorene, tri Phenylamine, carbazole and the like. These can be used individually or in mixture of 2 or more types.

According to certain embodiments of the invention, the radical polymerization initiator is 1-hydroxy cyclohexyl phenyl ketone (HCPK).

The polymerization initiator according to an embodiment of the present invention is peroxodisulfate calcium or titanium dioxide, and when peroxodisulfate calcium is used as the polymerization initiator, quaternary ammonium ions or N, N, N, N-tetramethyl as a catalyst Ethylene diamine can be used.

Since the monomer, the crosslinking agent and the radical polymerization initiator according to the present invention are for forming a hydrogel, so long as it is possible to form a crosslinkable hydrophilic polymer material, the kind of the monomer, the crosslinking agent and the polymerization initiator is not limited. It is preferred to dissolve in a monophasic.

The laponite is specifically Laponite XLG.

The second aspect of the present invention relates to a method of propagating marine life using a nanocomposite hydrogel,

Preparing a monomer;

Preparing an aqueous monomer solution using the monomer;

Advancing the reaction by adding a polymerization initiator as a crosslinking agent to the aqueous monomer solution;

Attaching marine organisms to the prepared hydrogel after completion of the reaction;

Attaching the hydrogel to which the marine organism is attached to an artificial reef structure;

It provides a method of propagating marine life, including.

The marine organisms are specifically marine plants, more specifically diatoms.

The present invention prepared a nanocomposite hydrogel. Representative examples of the basic monomers of the hydrogel according to the present invention are N, N'-dimethyl acrylamide, and an example of a crosslinking agent for crosslinking thereof is N, N'-methylenebisacrylamide or laponite.

Hydrogel according to the present invention showed a swelling degree similar to the swelling degree of the conventional hydrogel, it can be seen that in the adhesive strength with the concrete structure, the adhesive strength is about 20 times, the adhesive energy is increased by about 300 times or more. In addition, as a result of measuring the adhesion of algae, the hydrogel according to the present invention showed an effect of greatly increasing the adhesion of diatoms.

1 is an enlarged photograph of Nitzchia spp., Which is a diatom (Diatom) used to determine the adhesion characteristics of marine life according to the present invention.
Figure 2 is an illustration of the results of measuring the adhesive force on the concrete structure of the hydrogel according to the present invention.
Figure 3 is an example result showing the degree of swelling degree according to the amount of crosslinking agent.
4 is a result showing the peel force according to the deformation according to the type of hydrogel according to the present invention.
5 is a result showing the peel force according to the deformation according to the concentration of laponite of the hydrogel according to the present invention.
6 and 7 are experimental results of the attachment of algae according to the present invention.

<Substances used>

Monomers for preparing the hydrogel according to the present invention is N, N'-dimethyl acrylamide (N, N'-dimethyl acrylamide, manufactured by TCI), N, N'-methylenebisacrylamide (hereinafter 'MBAA') as a crosslinking agent. ', TCI) or Laponite XLG, the polymerization initiator was used peroxodisulfate calcium (potassium peroxodisulfate (hereinafter' KPS ', Sigma-Aldrich)).

All references to laponite, laponite XLG, laponite and the like as crosslinking agents throughout the specification of the invention all refer to the same laponite XLG.

N, N, N, N-tetramethylethylene diamine (N, N, N, N-tetramethylethylene diamine, hereinafter, 'TEMED', manufactured by Sigma-Aldrich) was used as a catalyst when using the KPS polymerization initiator. In addition, in order to compare the adhesion and adhesion characteristics of the initial reaction using the polymerization initiator of inorganic material instead of the organic initiator KPS, titanium (IV) (TiO ₂ , Sigma-) which is a mixture of rutile and anatase Aldrich) was used to prepare a hydrogel (DMAAm / Laponite / TiO ₂ hydrogel, hereinafter 'DLTi hydrogel').

To confirm the bio-fouling of marine microalgae on the surface of the hydrogel, diatom (Diatom) was used as Nitzchia spp. (See FIG. 1). Nitzschia species are diatoms that frequently appear in coastal or sea areas. The chloroplasts are divided into the upper and lower parts of the body and have a size of 30 to 50 μm, which is one of the algae that grow by dichotomy when attached to the surface of the material. The adhesion of was confirmed. Algae were cultured using F / 2 medium (CCAP), a seawater culture.

<Production of Hydrogel>

Example 1 Preparation of DMAAm-MBAA Hydrogel

Preparation of DMAAm-MBAA hydrogel (hereinafter 'DM hydrogel') was carried out by the following method.

The monomer DMAAm 10wt% (w / v), the organic crosslinking agent MBAA 1mol%, the oxidation-reduction polymerization initiator KPS 0.5wt% (w / v) was added to the distilled water and stirred for about 5 minutes. After degassing using a vacuum pump, 0.01 wt% (w / v) of TEMED, which is a catalyst, is completely dispersed, and then poured into a mold prepared by using a 2 mm thick silicone mold between 100 mm x 100 mm glass plates and room temperature. Polymerization was carried out for 5 hours.

Throughout the specification of the present invention, wt% (w / v) is the solute of 100 g of the corresponding solution, and mol% is the solute of the molar of 1000 ml of the solution.

Example 2 Preparation of DMAAm-Laponite Hydrogel

DMAAm-laponite hydrogel (hereinafter referred to as 'DL hydrogel') uses the same amount of monomer and polymerization initiator as in Example 1, and adds 5 wt% (w / v) of laponite XLG (w / v) instead of the organic crosslinking agent. Prepared in the same manner.

Example 3 DMAAm-Laponite-TiO ₂  Preparation of Hydrogel

DMAAm-laponite-TiO ₂ hydrogel (DLTi hydrogel) was used in the same manner as in Example 2 except that TiO ₂ was used to form a radical by light energy instead of KPS to cause photoinitiation reaction.

Post-treatment of hydrogels

Each prepared hydrogel was swelled in secondary distilled water for 6 days and distilled water was replaced every day to remove unreacted residue. Hydrogels for the algae attachment test was then swelled for three more days in seawater, cut into wells of six well plates, and divided into six for each sample.

<Swelling degree measurement>

Swelling is one of the methods for measuring how much water the hydrogel contains. The swelling degree of the hydrogel is measured by comparing the volume before and after the swelling and the method of comparing the weight. In this study, the swelling degree was measured using the weight comparison method.

<Physical property measurement>

Physical properties were measured and compared to compare the properties of DM hydrogel, DL hydrogel, and DLTi hydrogel. In order to measure the physical properties, a compression test, a tensile test, and a peel test were performed, and each hydrogel was performed five times (n = 5). The compression test was performed using Universal Testing Machine (AGX-plus, Simadzu, Japan), the load cell was 500N and the compression rate was 5mm / min to measure the Young's modulus of the hydrogel. In the tensile test, the fracture strain (mm / mm) and fracture stress (㎪) of the hydrogel were measured using the same apparatus, and the tensile velocity was 100 mm / min.

Peel test for confirming the adhesion of the hydrogel to the surface of the other material was carried out 90 ° peel test in accordance with ASTM D3330 to measure the adhesive strength. Hydrogel was prepared on the concrete structure in size of 40mm x 70mm and then tested at 60mm / min peeling rate. Through this, the jig moved to the length (mm) and the force (N) measured at this time. Peeling force (N) and peeling energy (peeling energy) (J) were measured to confirm adhesion to the material (see FIG. 2). In addition, to determine the mechanical properties of the hydrogel concentration of laponite XLG and the physical properties were measured by changing the laponite XLG concentration in the DLTi hydrogel.

<Fouling Test>

Diatoms are marine autotrophic protists, and when attached to a material, they are asexually reproduced by dichotomy. Therefore, the fouling test was performed using Nitzschia (see FIG. 1), which is a kind of diatoms, because it was determined that adhesion and propagation of the diatoms could be confirmed. Nitzschia was cultured in a mixed culture of seawater (98 wt%) and F / 2 medium (2 wt%).

The fouling test was conducted by comparing DM hydrogel, DL hydrogel and DLTi hydrogel to each well size of 6 well plates in order to compare the adhesion of the algae to the material. polystyrene). Experimental conditions were adjusted to 20 ℃, pH 8, sunlight (4000 ~ 6000lux, 12: 12hr), unmixed, the initial population was seeded by about 5 individuals per well by adjusting the concentration of algae 16 The daily change in the population of algae was measured (n = 6). After 3 days, the change in the population of algae was determined by averaging the number of Nitzschia attached per unit volume (mm 2) by specifying four random points on the sample per well. The microscope used Model CKX41SF (OLYMPUS CORPORATION, JAPAN).

<Result>

<1. Swelling Measurement>

Figure 3 shows one embodiment measuring the swelling degree of the hydrogel prepared using laponite XLG instead of the organic crosslinking agent. As the amount of crosslinking agent increased, the degree of swelling decreased. If the amount of cross-linking agent is increased, the network structure of the hydrogel is formed more densely, and the amount of water contained therein is analyzed to be smaller.

Subsequent comparative experiments showed similar swelling values for DM hydrogels, DL hydrogels, and DLTi hydrogels to compare physical properties, adhesion to materials, and algae adhesion of each hydrogel at similar water content (18, 21, 23) was prepared and compared.

<2. Comparison of Initial Elastic Modulus>

The Young's modulus of the materials was measured by compression tests of DM, DL, and DLTi hydrogels under similar water contents. The modulus of elasticity is a measure of the hardness of a material and represents the force per unit area required to deform the material. The modulus of elasticity of DM hydrogel was 149 ± 15.3㎪, DL hydrogel was 11.9 ± 1.3㎪, and DLTi hydrogel was 1.68 ± 0.2㎪.

The initial modulus of elasticity was decreased when the hydrogel was prepared using laponite as an inorganic material instead of the organic crosslinking agent (Example 2). This means that the shape of the material is more easily deformed when the material is subjected to the same force. In addition, it was confirmed that the initial modulus of elasticity was further reduced when the polymerization initiator used TiO ₂ as an inorganic compound instead of KPS as the organic polymerization initiator (Example 3). It was observed that the initial modulus increased with increasing the concentration of laponite at the same monomer and polymerization initiator ratios. It can be seen that Example 1 and Example 2 according to the present invention has a higher initial modulus value compared to Example 3.

<3. Comparison of Maximum Elongation and Breaking Strength>

Tensile strength and tensile strength of the material were measured by the tensile test. The maximum elongation was 0.71 ± 0.1 mm / mm for DM hydrogel (Example 1), 3.7 ± 0.5 mm / mm for DL hydrogel (Example 2), and 10.6 ± 1.1 mm / mm for DLTi hydrogel (Example 3). Appeared.

It was confirmed that the nanocomposite hydrogel according to the present invention was modified up to about 10 times the initial length. The breaking strength of DM hydrogel was 20.9 ± 1.4㎪, DL hydrogel 18.8 ± 3.9㎪, DLTi hydrogel 86.8 ± 21㎪, and the polymerization initiator was increased 4 times when TiO ₂ was used. Similarly, when the maximum elongation was measured according to the concentration of laponite (3-10 wt%), the elongation increased with increasing laponite concentration up to 5wt%, but decreased with subsequent concentration, and the breaking strength value increased in proportion to the concentration. Confirmed.

<4. Adhesion Comparison>

Adhesion (peeling force) and adhesion energy of the hydrogel to concrete structures As shown in FIG. 4, the adhesion strengths of 0.2 ± 0.1, 3.3 ± 0.3, 3.0 ± 0.2 N and 0.85, 309.5, respectively in DM, DL, and DLTi hydrogels, respectively. 541.9 mJ adhesion energy was measured. Comparing with this, it can be seen that the adhesive strength of DL hydrogel is increased by about 20 times and the adhesion energy is increased by about 300 times compared to DM hydrogel. In addition, as a result of measuring the adhesion strength and the adhesion energy according to the concentration change in the range of laponite 3-10 wt% as shown in FIG. 5, it was confirmed that the adhesion strength was reduced at a predetermined amount or more at 5 wt%.

<5. Fouling Properties>

The fouling properties of attached diatoms, which are the most important characteristics of the present invention, were investigated. Nitzschia has a growth phase, retardation phase, and death phase after inoculation. Here, the growth phase refers to the time when algae are rapidly increasing through the dichotomy, the retardation refers to the time when the population is maintained by the growth and killing of the algae, and the killing time refers to the speed at which the algae dies faster.

6 and 7 are the results of observing the adhesion of algae on the hydrogel and PS surface according to the present invention. (A-d) Polystyrene (e-h) DM hydrogel (i-l) DL hydrogel (m-p) DLTi hydrogel in each designation. Surfaces on Day 1, Day 3, Day 7, Day 16, respectively, on the longitudinal axis.

Sedimentation diatoms do not attach to the surface of the material, so the population cannot increase, resulting in faster retardation and killing. As a result of the measurement of the adhesion period of algae attached to the surface of the material over time, the growth period appeared in about 3 days in the material except the DM hydrogel, the number of the attached population rapidly increased. DM hydrogels showed a growth phase at about 5 days. The growth period was also shortest in DM hydrogels (about 1 day) and longest in DL hydrogels (about 12 days). As a result of measuring the population of algae attached to each hydrogel, the number of populations on the surface of DL hydrogel and DLTi hydrogel in which laponite is present was attached to and grown at least three times the surface of PS. On the other hand, algal populations attached to the DM hydrogel surface with MBAA were reduced by less than half compared to the PS surface (see FIG. 7). As a result, it was confirmed that the algae population was further increased on the surface of the hydrogel in which laponite was present.

Claims (10)

delete delete delete delete 1) preparing a monomer of the nanocomposite hydrogel;
2) preparing an aqueous monomer solution of the nanocomposite hydrogel using the monomer of the nanocomposite hydrogel;
3) advancing the reaction by adding a crosslinking agent and a polymerization initiator to the aqueous monomer solution of the nanocomposite hydrogel;
4) attaching marine organisms to the nanocomposite hydrogel prepared after completion of the reaction;
5) attaching the nanocomposite hydrogel to which the marine organism is attached to an artificial reef structure;
As the present invention relates to a method for proliferating marine life using a nanocomposite hydrogel that has improved the adhesion of the artificial reef structure for the growth of marine life, including,
Monomers of the nanocomposite hydrogel are N, N'-dimethylacrylamide (N, N'-diemthylacrylamide; DMAAm), N-isopropylacrylamide, acrylamide, vinylpyridine, vinylpyrrolidone, glycerol methacrylate, At least one of methoxy polyethylene glycol methacrylate, 2-hydroxyethyl acrylate, vinyl alcohol, methacrylic acid and acrylic acid,
The crosslinking agent of the nanocomposite hydrogel is N, N'-methylenebisacrylamide (MBAA), polyethylene glycol dimethacrylate, polyethylene glycol diacrylate, ethylene glycol dimethacrylate, Marine life of at least one of ethylene glycol diacrylate, diethylene glycol dimethacrylate, diethylene glycol diacrylate, triethylene glycol dimethacrylate and triethylene glycol diacrylate, laponite A method for propagation of marine organisms using nanocomposite hydrogels with improved adhesion of artificial reef structures for propagation. The method of claim 5,
The polymerization initiator is calcium peroxodisulfate, titanium dioxide, benzoin, benzoin methyl ether, benzoin ethyl ether, benzoin isopropyl ether, benzoin-n-butyl ether, benzoin isobutyl ether, acetophenone, hydroxide Roxydimethylacetophenone, dimethylaminoacetophenone, dimethoxy-2-phenylacetophenone, 3-methylacetophenone, 2,2-dimethoxy-2-phenylacetophenone, 2,2-diethoxy-2-phenylacetophenone , 4-Chronolocetophenone, 4,4-dimethoxyacetophenone, 2-hydroxy-2-methyl-1-phenylpropan-1-one, 4-hydroxycyclophenyl ketone, 1-hydroxycyclohexylphenyl Ketone, 2-methyl-1- [4- (methylthio) phenyl] -2-morpholino-propane-1-one, 4- (2-hydroxyethoxy) phenyl-2- (hydroxy-2- Propyl) ketone, benzophenone, p-phenylbenzophenone, 4,4-diaminobenzophenone, 4,4'-diethylaminobenzophenone, dichlorobenzophenone, anthraquinone, 2-methylanthraquinone, 2-ethylanthra Quinone , 2-t-butyl anthraquinone, 2-aminoanthraquinone, 2-methyl thioxanthone, 2-ethyl thioxanthone, 2-chlorothioxanthone, 2,4-dimethyl thioxanthone, 2,4-di Ethyl thioxanthone, benzyl dimethyl ketal, diphenyl ketone benzyl dimethyl ketal, acetophenone dimethyl ketal, p-dimethylaminobenzoic acid ester, 2,4,6-trimethylbenzoyl-diphenyl-phosphine oxide, fluorene, triphenylamine And propagation of marine organisms using nanocomposite hydrogels with improved adhesion of artificial reef structures for propagation of marine organisms of at least one of carbazoles. The method of claim 5,
Nanocomposites with improved adhesion of artificial reef structures for the proliferation of marine organisms when quaternary ammonium ions or N, N, N, N-tetramethylethylene diamine are used as catalysts when peroxodisulfate calcium is used as polymerization initiator Propagation method of marine life using hydrogel. The method of claim 5,
The laponite is a growth method of marine life using a nanocomposite hydrogel to increase the adhesion of the artificial reef structure for the growth of marine life that is laponite XLG. The method of claim 5,
The marine life is a method of propagating marine life using a nanocomposite hydrogel to increase the adhesion of the artificial reef structure for the growth of marine life as a marine plant. The method of claim 9,
The marine plant is a growth method of marine life using a nanocomposite hydrogel to increase the adhesion of the artificial reef structure for the growth of marine life of diatoms.

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