KR101990599B1 - Dna processing apparatus - Google Patents

Abstract

A DNA processing apparatus is disclosed, wherein the DNA processing apparatus includes a cassette including a plurality of pipette tips and a pipette tip preparation unit for preparing a target solution; A processing region in which a plurality of processing chambers are divided into a first column and a second column and a PCR processing region located at one end of the processing region, the other end of the processing region facing the PCR processing region is the pipette tip preparation An experimental kit mounted on the cassette so as to be disposed adjacent to the cassette; And a pipette tip transferring unit for coupling the pipette tip containing the object solution from the pipette tip preparation unit and moving the object solution in the pipette tip to perform the DNA treatment process on the experiment kit, A plurality of processes required for DNA processing may be performed on the first and second columns of the substrate.

Description

DNA PROCESSING APPARATUS

The present invention relates to a DNA processing apparatus.

DNA or RNA amplification technology, which is an essential step in the field of biological research, has been extensively used for research and development and diagnosis purposes in life sciences, genetic engineering, and medical fields. In particular, polymerase chain reaction Quot; DNA amplification technology ") is widely used.

However, PCR requires a large amount of purified nucleic acid. As a result, there is a limit to the separation of biological materials or nucleic acids by the researchers 'manuals at universities' laboratories or corporate laboratories. In order to overcome such limitations, automated devices for extracting biological substances or nucleic acids from biological samples have been manufactured . In addition, a variety of DNA processing processes have been performed, including a series of assays and confirmation of results, using DNA extracted and amplified by PCR.

However, conventionally, there are problems in that the process efficiency of the nucleic acid extraction, the PCR process, and the series of process steps using the extracted DNA are separately performed in separate devices, resulting in inconvenience to the user. In addition, since the sample is transferred to the PCR device by human hands after the nucleic acid extraction process is performed, contamination may occur or accuracy may be lowered when a large number of samples are processed.

The background technology of the present application is disclosed in Patent Registration No. 10-1700624.

It is an object of the present invention to provide a DNA processing apparatus capable of performing pre-processing of PCR, post-processing of PCR, and PCR with a simplified structure.

It should be understood, however, that the technical scope of the embodiments of the present invention is not limited to the above-described technical problems, and other technical problems may exist.

As a technical means for achieving the above technical object, a DNA processing apparatus according to the first aspect of the present invention comprises: a cassette including a plurality of pipette tips and a pipette tip preparation unit in which a target object solution is prepared; A processing region in which a plurality of processing chambers are divided into a first column and a second column and a PCR processing region located at one end of the processing region, the other end of the processing region facing the PCR processing region is the pipette tip preparation An experimental kit mounted on the cassette so as to be disposed adjacent to the cassette; And a pipette tip transferring unit for coupling the pipette tip containing the object solution from the pipette tip preparation unit and moving the object solution in the pipette tip to perform the DNA treatment process on the test kit, The first column and the second column of the kit may be subjected to a plurality of processes required for DNA processing.

In the DNA processing apparatus according to the first embodiment of the present invention, the remainder treatment chamber in the first column of the test kit is configured such that the object solution in the first treatment chamber is introduced into the remainder treatment chamber in the second row of the test kit, And the rearmost processing chamber of the second column may be connected to the PCR processing region through a capillary so that solution exchange with the PCR processing region is performed.

In addition, in the DNA processing apparatus according to the second embodiment of the present application, the remaining processing cells other than the rearmost processing chamber in the first row are subjected to pre-PCR processing, and the rearmost processing chamber and the forefront processing chamber The process can be performed after the PCR process.

Further, in the DNA processing apparatus according to the third embodiment of the present application, a DNA extraction process is performed in at least a part of the processing chambers other than the rearmost processing chamber in the first row, and the pipette tip transfer section The pipette tip in which the object solution is received is moved from the front to the rear of the processing container except for the rear processing container of the first row so that the DNA extraction process is performed, The object solution through the DNA extraction process can be accommodated for treatment.

In the DNA processing apparatus according to the fourth embodiment of the present invention, a DNA assay process is performed in at least a part of the processing chambers except the rearmost processing chamber and the foremost processing chamber among the processing chambers in the second row, Wherein the transfer unit is a pipetting unit for pipetting the object solution after the PCR treatment so that the pipette tip accommodates the object solution after the PCR treatment in the rearmost treatment chamber of the second row and then performs the DNA Assay process for the object solution after the PCR treatment, The tip may be moved from the rear-end processing chamber of the second row to the rear of the remaining processing chamber in the forward direction, and the object solution that has undergone the DNA Assay process may be accommodated in the foremost processing chamber of the second row.

In addition, the DNA processing apparatus according to the fifth embodiment of the present invention may further include: an optical sensor positioned below the cassette for identifying a target solution through the DNA assay process contained in the forefront processing chamber of the second row; And a PCR processing unit for performing a PCR process on the target solution introduced into the PCR processing region in association with the PCR processing region.

In addition, the DNA processing apparatus according to the sixth embodiment of the present invention is capable of moving in the forward and backward directions along the first and second rows on the experimental kit, and includes a plurality of magnetic bodies for magnetic processing during the DNA extraction process The magnetic module may further include a magnetic module.

In addition, in the DNA processing apparatus according to the seventh embodiment of the present invention, a plurality of test kits are mounted on the cassette, and the pipette tip preparation unit includes a first pipette tip and a second pipette tip 2 pipette tips.

Further, in the DNA processing apparatus according to the eighth embodiment of the present application, the processing chamber in the first column and the processing chamber in the second column of the experimental kit are subjected to different processes, and the different processes include an immune reaction process and a molecular diagnosis Process.

In addition, in the DNA processing apparatus according to the ninth embodiment of the present invention, the pipette tip transferring unit may be configured such that the pipette tip is fastened to perform the process in the processing chamber of the first row, After the process, the second pipette tip may be tightened to perform the process in the treatment column of the second row and move on the test kit.

The above-described task solution is merely exemplary and should not be construed as limiting the present disclosure. In addition to the exemplary embodiments described above, there may be additional embodiments in the drawings and the detailed description of the invention.

According to the above-mentioned task solution of the present invention, the pre-PCR treatment can be performed in other treatment chambers except the rearmost treatment chamber of the first row of the experimental kit, so that the object solution subjected to the pre- And the PCR-treated solution can be introduced into the remainder treatment chamber of the second row. Therefore, the solution which has been introduced into the rearmost treatment chamber of the second row The PCR treatment solution can be transferred to the foremost processing chamber of the second row through the other processing chambers sequentially except for the rearmost processing chamber of the second row. Since such a process can be unified and performed in one apparatus, the contamination of the object solution can be prevented, and the efficiency of the PCR treatment can be improved.

1 is a schematic perspective view of a DNA processing apparatus according to an embodiment of the present invention;
2 is a schematic perspective view of a cassette equipped with an experimental kit of a DNA processing apparatus according to an embodiment of the present invention;
3 is a schematic plan view of a cassette equipped with an experimental kit connected to a PCR processing unit of a DNA processing apparatus according to an embodiment of the present invention.
4 is a schematic perspective view of a pipette tip transfer unit of a DNA processing apparatus according to an embodiment of the present invention;

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily carry out the present invention. It should be understood, however, that the present invention may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. In the drawings, the same reference numbers are used throughout the specification to refer to the same or like parts.

Throughout this specification, when a part is referred to as being "connected" to another part, it is not limited to a case where it is "directly connected" but also includes the case where it is "electrically connected" do.

It will be appreciated that throughout the specification it will be understood that when a member is located on another member "top", "top", "under", "bottom" But also the case where there is another member between the two members as well as the case where they are in contact with each other.

Throughout this specification, when an element is referred to as "including " an element, it is understood that the element may include other elements as well, without departing from the other elements unless specifically stated otherwise.

For reference, the terms related to directions and positions (front, rear, etc.) in the description of the embodiments of the present application are set based on the arrangement states of the respective structures shown in the drawings. For example, referring to FIG. 2, the 2 o'clock direction may be generally front, and the 8 o'clock direction generally may be rearward. Conversely, when viewed from Fig. 1, the 8 o'clock direction as a whole can be forward, and the 2 o'clock direction as a whole can be rearward

The present invention relates to a DNA processing apparatus. For reference, the DNA processing apparatus according to one embodiment of the present invention is not limited to DNA processing, but can be used for processing other biological components such as nucleic acid besides DNA processing.

Hereinafter, a DNA processing apparatus (hereinafter referred to as a " present processing apparatus ") according to an embodiment of the present invention will be described.

FIG. 1 is a schematic perspective view of a DNA processing apparatus according to an embodiment of the present invention, FIG. 2 is a schematic perspective view of a cassette equipped with an experimental kit of a DNA processing apparatus according to an embodiment of the present invention, and FIG. FIG. 4 is a schematic perspective view of a pipette tip transfer unit of a DNA processing apparatus according to an embodiment of the present invention. FIG. 4 is a schematic plan view of a cassette equipped with an experimental kit connected to a PCR processing unit of a DNA processing apparatus according to an embodiment.

Referring to Fig. 1, the present processing apparatus includes a cassette 1. Fig. Referring to Fig. 1, the processing apparatus may include a plurality of cassettes 1. Fig. Illustratively, referring to FIG. 1, the present processing apparatus may include four cassettes 1. The cassette 1 is detachable from the tray of the apparatus main body and can move in the back and forth direction.

1 and 2, a cassette 1 includes a plurality of pipette tips 111 and a pipette tip preparation unit 11 in which a target solution is prepared. The object solution can be received in the test tube 112 and prepared in the pipette tip preparation section 11. [ Illustratively, the subject solution may be a serum formed by centrifugation. Also, for example, the pipette tip 111 may be a 1 ml tip. 2, a plurality of pipette tips 111 may be provided for each experimental kit 2, and one test tube 112 may be provided for each of the pipette tips 111. As shown in FIG. 2, . Two pipette tips 111 may be provided corresponding to the number of the first row 21 and the second row 22, which are two processing lines provided in the experimental kit 2. [

The positions of the plurality of pipette tips 111 and the test tubes 112 are determined in accordance with the order of the pipette tip transferring unit 3 to be described later, the clamping of the pipette tip 111, the acceptance of the target object solution, . Two pipette tips 111 and a test tube 112 corresponding to each test kit 2 on the cassette 1 can be sequentially arranged in the direction toward the test kit 2 as shown in Fig. . The collision between the pipette tip 111 and the test tube 112 during the process of moving the pipette tip transfer unit 3 in the vertical direction after the pipette tip transfer unit 3 is tightened by the pipette tip transfer unit 3 is minimized The height of the arrangement positions of the two pipette tips 111, the test tube 112, and the test kit 2 on the cassette 1 may be different. For example, in consideration of the moving direction of the pipette tip transfer part, the position of the test tube 112 may be relatively lower than that of the pipette tip 111 and the placement position of the test kit 2 may be located at the lower end. For example, the test tube 112 may be located at an inclined portion of the cassette 1 (of the pipette tip preparation portion 1).

Further, referring to Fig. 1, the present treatment apparatus includes an experimental kit (2). 1 and 2, the experimental kit 2 includes processing areas 21 and 22 in which a plurality of processing chambers are divided into a first column 21 and a second column 22, And a PCR processing region 23 located at one end of the end of the PCR. 2, the experimental kit 2 is designed such that the other end 23 of the processing region 21, 22 facing the PCR processing region 23 is arranged adjacent to the pipette tip preparation portion 1 And is mounted on the cassette 1. In other words, the experimental kit 2 can be mounted on the cassette 1 such that one end of the processing areas 21 and 22 is directed to the pipette tip preparation part 11 and the other end is connected to a PCR processing part not shown . Further, a plurality of test kits 2 may be mounted on the cassette 1. [ Illustratively, four experimental kits 2 can be mounted. Accordingly, according to the DNA processing apparatus according to one embodiment of the present invention, a total of 16 DNAs can be simultaneously treated. A sensor for detecting at least one of the mounting state and the mounting state of each of the plurality of test kits 2 may be provided in the mounting portion on which the plurality of test kits 2 of the cassette 1 are mounted.

1, the present processing apparatus includes a pipette tip transferring section 3. As shown in Fig. The pipette tip transferring unit 3 has a pipette tip 111 for receiving a target object solution from the pipette tip preparing unit 11 and a target object solution is accommodated in the pipette tip 111 to perform a DNA processing process (2). Referring to FIG. 1, the initial position of the pipette tip transfer part 3 may be the rear of the test kit 2. The pipette tip transferring part 3 located at the rear of the test kit 2 can be moved forward toward the pipette tip preparing part 11 and moves to the upper part of the pipette tip to be fastened and descends to the pipette tip preparing part 11 The pipette tip 111 can be gripped (fastened). In addition, at least a portion of the gripped pipette tip 111 can be drawn into the test tube 112 containing the object solution to accommodate the object solution in the test tube 112 into the pipette tip 111. 4, the pipette tip transferring unit 3 may further include a syringe for receiving and discharging the object solution into the pipette tip 111, and the like. The pipette tip 111 containing the object solution is moved on the experimental kit 2 so that the object solution can be processed in each treatment chamber of the experimental kit 2 when the object solution is contained in the pipette tip 111 . The movement of the pipette tip 111 on the experimental kit 2 means that the pipette tip 111 is driven to inject at least a part of the solution therein into the at least one processing chamber, , A drive for sucking at least a part of the solution in at least one processing chamber, etc., and moving in the forward and backward direction through the plurality of processing chambers.

More specifically, the treatment process of the object solution for the experimental kit (2) may be as follows.

Referring to FIG. 2, in the first column 21 and the second column 22 of the experimental kit 2, a plurality of processes required for DNA processing can be performed.

Illustratively, the pre-PCR processing can be performed in the remaining processing chambers except the rearmost processing chamber 211 of the first column 21. [ Illustratively, a DNA extraction process may be performed in at least some of the remaining treatment chambers except for the remainder treatment chamber 211 of the first column. To this end, a solution for the DNA extraction process can be accommodated in the processing chamber where the DNA extraction process is performed among the remaining processing chambers except the rearmost processing chamber 211 of the first column 21. [ Illustratively, at least some of the remaining treatment chambers except the rearmost treatment chamber 211 of the first row 21 may be subjected to a washing process. The washing process may include a step of breaking bacteria and viruses in the object solution to separate them from DNA. To this end, the washer fluid may be accommodated in a space where the washing process is performed among the remaining process chambers except the rearmost treatment chamber 211 of the first row 21. Also, for example, the number of cells in which the washing process is performed may be three. In addition, in at least some of the remaining processing chambers except the rearmost processing chamber 211 of the first column 21, a DNA extraction process using beads can be performed.

The pipette tip transferring unit 3 transfers the pipette tip 111 in which the object solution is received to the remaining processing chambers except the rearmost processing chamber 211 of the first row 21 so that the DNA extraction process for the object solution is performed. It can be moved from the front direction to the rear direction.

That is, the pipette tip transferring part 3 is configured such that the pipette tip 111 can transfer at least a part of the solution contained therein to the remainder treatment chamber 211 of the first row 21 , And a drive for sucking at least a portion of the solution in the remaining treatment chambers, respectively, to the respective processing chambers, and the second processing chamber is moved from the foremost processing chamber of the first column (21) (Left arrow direction in Fig. 2)

Accordingly, a DNA extraction process, a washing process, and the like can be performed on the object solution contained in the pipette tip 111.

For reference, referring to FIG. 1, the present DNA processing apparatus may include a plurality of pipette tip transferring units 3. In addition, each of the plurality of pipette tip transferring portions 3 may be driven corresponding to each of the plurality of cassettes 1 in a one-to-one correspondence. Illustratively, as described above, when four cassettes 1 are provided, each of the four pipette tip feeders 3 may be provided for each of the four cassettes 1. [ In addition, the pipette tip transferring unit 3 is equipped with a plurality of pipette tips 11, and operations for each of the pipette tips 11 can be performed. Illustratively, referring to FIG. 4, four pipette tips 11 are mountable in the pipette tip transfer section 3.

1, the present treatment apparatus is capable of moving in the forward and backward directions along the first row 21 and the second row 22 on the experimental kit 2, and has a plurality of And a magnetic module 6 including a magnetic body. The magnetic module 6 approaches the pipette tip 111 gripped by the pipette tip transfer part 3 within a distance that the magnetic force of the magnetic module 6 can act on the pipette tip 111 and moves the pipette tip 111 gripped by the pipette tip transfer part 3, The magnetic force can be applied. Further, by moving away from the pipette tip 111 gripped by the pipette tip transferring unit 3, the magnetic force on the pipette tip 111 gripped by the pipette tip transferring unit 3 can be released. Further, illustratively, magnetic beads may be included in some treatment cells of the first row 21 and the second row 22 of the experimental kit 2. Accordingly, the magnetic module can maintain and extract the DNA binding of the magnetic bead-bound object solution by applying a magnetic force (magnetism) to the pipette tip 111 gripped by the pipette tip transferring part 3. When the magnetic beads and DNA are combined, the DNA-bound magnetic beads can be separated from the other components. For example, after the magnetic module 6 fixes the magnetic beads and removes the other solution, the magnetic beads to which the DNA is bound can be separated from the other components. In addition, the magnetic module releases the position fixation of the magnetic beads to which the DNA is bound, so that the magnetic beads to which the DNA is bonded are accommodated in the washing solution accommodated in at least one of the remaining processing chambers except for the rearmost processing chamber of the first row , The contaminants bound to the magnetic beads to which the DNA is bound can be washed. By such a process, the above-described DNA extraction process, washing process, and the like can be performed.

In addition to these processes, according to the present treatment apparatus, the step of eluting DNA from the magnetic beads to which the DNA is bound, the step of molecular enrichment of the eluted DNA, and the like can be performed.

For reference, the magnetic module 6 may include a magnetic material. For example, the magnetic material may be a magnet, and more specifically, an electromagnet. If the magnetic material is an electromagnet, the magnetism of the magnetic material may be lost or regenerated by turning on / off the current flowing through the electromagnet.

Referring to FIG. 3, the pipette tip transferring unit 3 can accommodate the object solution subjected to the DNA extraction process for the PCR treatment in the remainder treatment chamber of the first row 21.

The remainder treatment chamber 211 of the first row 21 of the test kit 2 is configured such that the target object solution in the first column 21 is placed in the remainder treatment chamber 221 of the second row 22 of the test kit 2, Processing chamber 221 of the second row 22 so as to be introduced into the rear- Although not shown, the remainder treatment chamber 221 of the second row 22 may be connected to the PCR processing region 23 through a capillary so that solution exchange with the PCR processing region 23 is performed.

That is, after the object solution is received in the pipette tip 111, the pipette tip transferring part 3 is moved backward from the foremost processing chamber of the first row 21, Or injecting the solution in the treatment chamber into the pipette tip 111, or the like. The pipette tip 111 may be brought into the rearmost treatment chamber 221 of the first row 21 and the second row of the second row 21 of the first row 21, An operation is performed in the processing chamber so that the solution contained in the pipette tip 111 can be injected into the remainder treatment chamber 211 of the first row 21. [ The solution injected into the remainder treatment chamber 221 of the first column 21 can be introduced into the remainder treatment chamber 211 of the second column 22 and the solution injected into the remainder treatment chamber 211 of the second column 22 The solution introduced into the PCR processing region 23 can be introduced into the PCR processing region 23 through the capillary. The solution introduced into the PCR treatment zone 23 can be introduced into the remainder treatment chamber 221 of the second row 22. [

Although not shown, the present processing apparatus may include a PCR processing unit for performing PCR processing on the object solution introduced into the PCR processing region 23 in cooperation with the PCR processing region 23. [ For example, the PCR processing section can be connected or connected to the PCR processing region 23. [ As described above, the solution introduced into the PCR treatment zone 23 from the remainder treatment chamber 221 of the second column 22 can be subjected to PCR treatment, and the PCR-treated solution is supplied to the second column 22 To the rear-end processing chamber 221 of the rear- For reference, the PCR processor can perform the synthesis and amplification of DNA by repeating the process of uniformly heating the target solution after the DNA extraction process to a specific temperature several times. 3, the present processing apparatus may further include a PCR connection unit 4 for connection with a PCR processing unit, and the PCR connection unit 4 may include a heating unit, a cooling unit, A cooling water inlet and outlet passage 41 for discharging the cooling water, and the like.

In addition, according to one embodiment of the present invention, a process after the PCR process can be performed in the remaining processing chambers except the rearmost processing chamber 221 and the forefront processing chamber 222 of the second row 22. [ Illustratively, at least some of the processing chambers of the second column 22, except for the last processing chamber 221 and the foremost processing chamber 222, use a series of DNA Assay processes using extracted and amplified DNA Lt; / RTI > Thus, the plurality of treatment chambers of the second row 22 can accommodate various reagents required for a series of DNA assays.

The pipette tip transferring unit 3 is configured such that the pipette tip 111 receives the object solution after the PCR treatment in the remainder treatment chamber 221 of the second row 22 and then performs DNA assays for the object solution after the PCR treatment The pipette tip 111 in which the object solution after the PCR treatment is accommodated is moved from the rear treatment chamber 221 of the second row 22 to the other treatment chamber in the forward direction (the pipette tip preparation section 11) And the object solution that has undergone the DNA assay process can be accommodated in the foremost processing chamber 222 of the second row 22.

The present processing apparatus can also include an optical sensor 5 located below the cassette 1 for identifying a solution of the object subjected to the DNA assay process contained in the foremost processing chamber 222 of the second row 22 have. For example, the optical sensor 5 may comprise a camera module.

In addition, illustratively, the fluorescent substance can be contained in at least a part of the processing chambers of the second row 22 except the rearmost processing chamber 221 and the foremost processing chamber 222, The object solution can be subjected to fluorescence treatment by the fluorescent substance through the remaining treatment cell. This may be to allow the object solution after the PCR treatment to be more easily sensed by the optical sensor 5 when it is sensed by the optical sensor 5 in the foremost processing chamber 222.

In addition, the pipette tip preparing section 11 can accommodate a first pipette tip and a second pipette tip for performing a plurality of DNA processing steps for each experimental kit 2. The pipette tip can be determined according to the kind of process performed in the experimental kit 2 corresponding to each experimental kit 2. According to one embodiment of the invention, the first pipette tip and the second pipette tip may be used in performing different processes. For example, a first pipette tip may be used to perform the process for the first row 21, and a second pipette tip may be used to perform the process for the second row 22. [ Alternatively, one pipette tip may be used to perform the process for the first row 21 and the second row 22. The pipette tip transferring part 3 thus has a first pipette tip fastened to the processing column of the first row 21 and a second pipette tip after the processing in the processing chamber of the first row 21 The second pipette tip may be tightened and moved on the test kit 2 for performing the process in the processing chamber of FIG. For example, the relatively forward positioned pipette tip may be the first pipette tip and the relatively later positioned pipette tip may be the second pipette tip.

Further, as described above, different processes may be performed on the processing chamber of the first column 21 and the processing chamber of the second column 22 of the experimental kit 2. The different processes may be the pre-PCR process and the post-PCR process, as described above.

Further, according to another embodiment of the present application, the different processes may include an immune reaction process and a molecular diagnostic process. For example, one of the first column 21 and the second column 22 may be subjected to an immunoreaction process and the other may be subjected to a molecular diagnostic process. In this case, the pipette tip transferring part 3 is connected to the pipette tip transferring part 3 after the first pipette tip is fastened to perform the process in the processing chamber of the first row 21 and after the process in the processing chamber of the first processing chamber 21, The pipette tip can be moved on the test kit 2 by tightening the second pipette tip to perform the process in the heat treatment chamber 22. [ More specifically, the pipette tip transferring unit 3 is configured such that the pipette tip is fastened to perform the process in the processing chamber of the first row 21, and the pipette tip transferring unit 3 is connected to the forefront processing chamber 211 of the first row 21 And returns to the pipette tip preparation unit 11 to perform the process in the processing chamber of the first column 21 and the process in the processing chamber 22 of the second row after the PCR process after the process in the processing chamber 21, 2 pipette tip is moved to the upper part of the remainder treatment chamber 211 of the second treatment row to accommodate the object solution and is then moved along the second treatment row 22 to the forefront treatment chamber 222 of the second treatment row 22 . ≪ / RTI >

As described above, according to the DNA processing apparatus according to one embodiment of the present invention, DNA processing for a plurality of objects can be simultaneously performed, and DNA extraction, amplification, PCR processing, assay processing, Can be performed in one processing apparatus. In addition, since a large amount of processing can be automatically performed without user intervention, it is possible to prevent unnecessary contamination, infection, and the like, which may occur during processing, and to improve the accuracy of processing.

It will be understood by those of ordinary skill in the art that the foregoing description of the embodiments is for illustrative purposes and that those skilled in the art can easily modify the invention without departing from the spirit or essential characteristics thereof. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims rather than the detailed description, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

1: Cassette
11: Preparing the pipette tip
111: Pipette tip
112: Experimenters
2: Experiment kit
21: First column
211: the last processing chamber in the first column
22: 2nd column
221: the second-row processing chamber
222: the frontmost processing chamber of the second row
23: PCR processing region
3: Pipette tip feeder
4: PCR connection
41: Cooling water inlet /
5: Optical sensor
6: Magnetic module

Claims (10)

In the DNA processing apparatus,
A cassette including a plurality of pipette tips and a pipette tip preparation part in which a target object solution is prepared;
A processing region in which a plurality of processing chambers are divided into a first column and a second column and a PCR processing region located at one end of the processing region, the other end of the processing region facing the PCR processing region is the pipette tip preparation An experimental kit mounted on the cassette so as to be disposed adjacent to the cassette; And
A pipette tip transferring unit for coupling the pipette tip, which receives the object solution from the pipette tip preparation unit, into the pipette tip and moving the object solution on the experiment kit to perform a DNA processing process,
, ≪ / RTI &
The rearmost treatment chamber of the first row of the experimental kit is connected to the rearmost treatment chamber of the second row so that the target object solution therein flows into the rearmost treatment chamber of the second row of the test kit,
The rearmost processing chamber of the second row is connected to the PCR processing region through a capillary so that solution exchange with the PCR processing region is performed,
The remaining processing chambers except the rearmost processing chamber in the first column are subjected to a plurality of processes for the pre-PCR processing,
The remaining processing chambers except the rearmost processing chamber and the foremost processing chamber in the second row are subjected to a plurality of processes for the post-PCR processing,
Wherein the pipette tip transferring portion sequentially processes a plurality of processes for the pre-PCR process in the remaining treatment chambers except for the remainder treatment chamber of the first row with respect to the object solution contained in the pipette tip, Wherein the pipette tip is moved so that a plurality of processes for the post-PCR processing are successively processed in the remaining processing chambers except the rearmost processing chamber and the forefront processing chamber of the column. delete delete The method according to claim 1,
A DNA extraction process is performed in at least a part of the processing chambers other than the rearmost processing chamber in the first row,
The pipette tip transferring portion moves the pipette tip in which the object solution is received from the front to the rear of the processing chamber excluding the rearmost processing chamber of the first row so that the DNA extraction process for the object solution is performed, Wherein the object solution passed through the DNA extraction step for the PCR treatment is contained in the rearmost processing chamber of the first column. 5. The method of claim 4,
A DNA assay process is performed in at least a part of the processing chambers of the second row except for the rearmost processing chamber and the foremost processing chamber,
Wherein the pipette tip transferring portion is configured to transfer the pipette tip to the object solution after the PCR treatment so that the pipette tip receives the object solution after the PCR treatment in the rearmost treatment chamber of the second row and then performs the DNA Assay process on the object solution after the PCR treatment. And the accommodated pipette tip is moved from the rearmost processing chamber of the second row to the rear of the remaining processing chamber in the forward direction and the object solution that has undergone the DNA Assay process is received in the foremost processing chamber of the second row, Processing device. 6. The method of claim 5,
An optical sensor located at a lower portion of the cassette for identifying a solution of the object which has been subjected to the DNA Assay process contained in the foremost processing chamber of the second row; And
A PCR processing unit for performing a PCR process on a target solution introduced into the PCR processing region in association with the PCR processing region,
Further comprising a DNA polymerase. 6. The method of claim 5,
A magnetic module including a plurality of magnetic bodies for magnetic processing during the DNA extraction process, the magnetic module being movable in the forward and backward directions along the first and second columns on the experiment kit,
Further comprising a DNA polymerase. The method according to claim 1,
A plurality of test kits are mounted on the cassette,
Wherein the pipette tip preparation portion accommodates a first pipette tip and a second pipette tip for performing a DNA treatment process for each experimental kit. 9. The method of claim 8,
The treatment kits of the first column and the treatment column of the second column of the experimental kit are subjected to different processes,
Wherein the different process comprises an immune reaction process and a molecular diagnostic process. 10. The method of claim 9,
Wherein the pipette tip transferring portion is configured to fasten the first pipette tip for performing a process in the processing chamber of the first row and to transfer the pipette tip to the processing chamber of the first row after the process in the processing chamber of the first row, 2 < / RTI > pipette tips are tightened and moved on the experimental kit.

Images