KR101881531B1 - Method for Preparing Stem Cells Having Improved Expression of Cytokines Using Poly-2-hydroxyethyl Methacrylate - Google Patents

Method for Preparing Stem Cells Having Improved Expression of Cytokines Using Poly-2-hydroxyethyl Methacrylate Download PDF

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Publication number
KR101881531B1
KR101881531B1 KR1020160158414A KR20160158414A KR101881531B1 KR 101881531 B1 KR101881531 B1 KR 101881531B1 KR 1020160158414 A KR1020160158414 A KR 1020160158414A KR 20160158414 A KR20160158414 A KR 20160158414A KR 101881531 B1 KR101881531 B1 KR 101881531B1
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KR
South Korea
Prior art keywords
cytokines
poly
stem cells
hydroxyethyl methacrylate
improved expression
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KR1020160158414A
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Korean (ko)
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KR20170104917A (en
Inventor
임도선
김종호
주형준
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고려대학교 산학협력단
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Priority to KR1020160158414A priority Critical patent/KR101881531B1/en
Publication of KR20170104917A publication Critical patent/KR20170104917A/en
Application granted granted Critical
Publication of KR101881531B1 publication Critical patent/KR101881531B1/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • C12N5/0667Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52Cytokines; Lymphokines; Interferons
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/003D culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/30Synthetic polymers

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
KR1020160158414A 2016-11-25 2016-11-25 Method for Preparing Stem Cells Having Improved Expression of Cytokines Using Poly-2-hydroxyethyl Methacrylate KR101881531B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020160158414A KR101881531B1 (en) 2016-11-25 2016-11-25 Method for Preparing Stem Cells Having Improved Expression of Cytokines Using Poly-2-hydroxyethyl Methacrylate

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020160158414A KR101881531B1 (en) 2016-11-25 2016-11-25 Method for Preparing Stem Cells Having Improved Expression of Cytokines Using Poly-2-hydroxyethyl Methacrylate

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020160027472 Division 2016-03-08

Publications (2)

Publication Number Publication Date
KR20170104917A KR20170104917A (en) 2017-09-18
KR101881531B1 true KR101881531B1 (en) 2018-07-25

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Application Number Title Priority Date Filing Date
KR1020160158414A KR101881531B1 (en) 2016-11-25 2016-11-25 Method for Preparing Stem Cells Having Improved Expression of Cytokines Using Poly-2-hydroxyethyl Methacrylate

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KR (1) KR101881531B1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102095346B1 (en) * 2018-11-16 2020-03-31 고려대학교 산학협력단 Adipose-derived Stem Cell Sheet for Transplantion with Enhanced Angiogenesis Potential by Three-dimensional Co-culutre

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003224950B2 (en) * 2002-04-09 2006-09-14 Biogen Ma Inc. Methods for treating tweak-related conditions
DE10362002B4 (en) * 2003-06-23 2006-10-12 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Adult pluripotent stem cells
WO2007095064A2 (en) * 2006-02-09 2007-08-23 Wisconsin Alumni Research Foundation ERYTHROID CELLS PRODUCING ADULT-TYPE β-HEMOGLOBIN GENERATED FROM HUMAN EMBRYONIC STEM CELLS
EP3613847A1 (en) * 2013-03-13 2020-02-26 The University of Queensland A method of isolating cells for therapy and prophylaxis

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