KR101865764B1 - A cosmetic composition for skin desquamation comprising natural complex extract and spicule powder - Google Patents

Abstract

The present invention relates to a cosmetic composition which contains: a complex extract consisting of a Mucor miehei extract, a papaya fruit extract, and a pumpkin extract; betaine salicylate; and spicule powder as effective components. An objective of the present invention is to provide a functional cosmetic composition which is less irritating to the skin and effectively removes dead skin cells of the skin.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for extracting keratin, which comprises a natural combination extract and a fine needle powder as an active ingredient. BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic composition for skin exfoliation,

The present invention relates to a cosmetic composition containing a natural combination extract and a micropowder powder as an active ingredient.

The stratum corneum is composed of 10 to 20 layers of dead cells and intercellular nerves, natural moisturizing factor (NMF) present in the outermost part of the skin, which protects the skin from external pollutants, Lt; RTI ID = 0.0 > of water < / RTI >

In healthy individuals, the differentiated cells from the basal layer mature and reach the granule layer. On the 14th day, the cells reaching the granule layer disappear and disappear, and it takes 14 days until they become keratinocytes and arrive at and disappear from the stratum corneum. The turn-over period of the skin stratum corneum is about 4 weeks. When a predetermined period elapses, the old stratum corneum is naturally peeled off and a new stratum corneum is formed.

However, due to internal factors such as aging and external factors such as ultraviolet rays, the turnover cycle becomes long and unbalanced, and the stratum corneum is old and stratified, resulting in a rough skin, uneven skin tone, and skin incongruity.

One of the signs of aging skin is a reduction in the ability to dislodge dead cells, which causes the skin to lose vitality and elasticity as the cell regeneration cycle increases. In order to improve this, efforts have been actively made to restore skin elasticity by stimulating cell regeneration of the basal layer by removing the skin layer through a skin peeling technique.

There are three kinds of exfoliating cosmetics. The first is a method of finely cutting the skin by physical action such as skin friction or laser. The second is a chemical method in which an AHA (Alpha-Hydroxy Acid), BHA (Beta- Hydroxy Acid), and urea, and the third is a method of peeling the keratin using a proteolytic enzyme having a function of exfoliation.

In physical removal, skin irritation is severe and it is not suitable for home use. In the case of chemical method, it is not effective because it is possible to use only low volume in household products. Protein peeling agents are expensive and ineffective.

Meanwhile, the cosmetics industry is developing a number of products using natural products to reduce skin irritation caused by various chemicals. In addition to low adverse effects on skin, natural materials have recently been increasingly developed as cosmetic raw materials, as consumers have become more receptive to cosmetics using natural materials.

Accordingly, the present inventors have developed an eco-friendly and highly functional cosmetic product for peeling off keratin which contains only natural ingredients corresponding to EWG 1 grade and can safely use sensitive skin.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and an object of the present invention is to provide a functional cosmetic composition which is less irritating to skin and effectively removes keratin.

According to one aspect of the present invention, there is provided a combination extract comprising a mucormiehei extract, a papaya fruit extract, and an amber extract; BETAINE SALICYLATE, and a fine needle powder as an active ingredient.

In one embodiment, the micropowder powder may be obtained from a sponge animal.

In one embodiment, the sponge animal may be a Spongilla lacustris .

In one embodiment, the average particle length of the fine needle-like powder may be 70 to 500 탆.

In one embodiment, the combined extract may be a fermentation extract obtained by inoculating a strain of Lactobacillus sp .

In one embodiment, the combined extract may comprise from 0.5 to 40% by weight of the total weight of the composition, and the micropowder powder may comprise from 0.1 to 5% by weight.

In one embodiment, the combined extract may comprise 10 to 30 parts by weight of the Mucor miehei extract, 10 to 30 parts by weight of the papaya fruit extract, and 10 to 30 parts by weight of the amber extract.

In one embodiment, the combined extract may be extracted with one or more solvents selected from the group consisting of purified water, lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol .

In one embodiment, the cosmetic composition is a composition comprising a softening agent, a convergent lotion, a nutritional lotion, a lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, ≪ / RTI >

The cosmetic composition according to one aspect of the present invention contains a natural extract as an active ingredient, so that skin irritation caused by a chemical preparation can be minimized and the exfoliation effect can be remarkably improved.

In particular, the natural raw material applied to the cosmetic composition of the present invention is a raw material corresponding to the EWG 1 grade and can be applied safely to sensitive skin without harmfulness to human body.

It should be understood that the effects of the present invention are not limited to the effects described above, but include all effects that can be deduced from the description of the invention or the composition of the invention set forth in the claims.

As used herein, the terminology used herein is intended to encompass all commonly used generic terms that may be considered while considering the functionality of the present invention, but this may vary depending upon the intent or circumstance of the skilled artisan, the emergence of new technology, and the like. Also, in certain cases, there may be a term selected arbitrarily by the applicant, in which case the meaning thereof will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term, not on the name of a simple term, but on the entire contents of the present invention.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.

The numerical range includes numerical values defined in the above range. All numerical limitations of all the maximum numerical values given throughout this specification include all lower numerical limitations as the lower numerical limitations are explicitly stated. All the minimum numerical limitations given throughout this specification include all higher numerical limitations as the higher numerical limitations are explicitly stated. All numerical limitations given throughout this specification will include any better numerical range within a broader numerical range, as narrower numerical limitations are explicitly stated.

Hereinafter, embodiments of the present invention will be described in detail, but it should be apparent that the present invention is not limited by the following examples.

One aspect of the present invention relates to a combination extract comprising a mucormiehei extract, a papaya fruit extract, and an amber extract; BETAINE SALICYLATE, and a fine needle powder as an active ingredient.

The cosmetic composition minimizes the chemical preparation and substitutes the chemical agent with an extract of natural origin, thereby achieving superior skin exfoliation and hypoallergenic properties.

As used herein, the phrase " comprising as an active ingredient " means containing an effective amount capable of exhibiting a skin improving effect, such as collagen synthesis associated with a wrinkle-reducing effect, elasticity improvement or skin soothing, exfoliating effect .

In particular, since the cosmetic composition contains only EWG level 1 components, it satisfies both functionality and safety, and is easy to apply to sensitive skin because it is not harmful.

EWG is a cosmetic ingredient harmful grade announced by EWG (Environmental Working Group), a nonprofit environmental NGO in the United States. EWG carefully examines the harmfulness of cosmetic ingredients and classifies the hazard level from 1 to 10, and 1 Class 2 and Class 2 are classified as safety class.

The term " exfoliating the skin " means that the keratin substances accumulated on the stratum corneum of the skin are removed or removed from the stratum corneum of the skin. The exfoliation can be performed not only by removing physical force but also by applying a cosmetic composition And the case where it is separated from the stratum corneum.

The term " hypoallergenic " means that the irritation of the skin such as redness and stinging of the skin is relatively reduced. The cosmetic composition of the present invention can be understood as a cosmetic composition for hypolipidemic detachment which maintains the exfoliation effect while inducing less redness or stinging of the skin as compared with a cosmetic containing only a high content of betaine salicylate.

The above-mentioned " Mucor miehei " is a proteolytic enzyme of a microorganism of Mucor belonging to less fungi. It has excellent proteolytic activity and can be used for aging cheese and the like when it is applied as a food. The mucormihei has excellent proteolytic activity, and therefore, when applied to a cosmetic composition, it excellently exfoliates the skin and can stimulate the skin because it is a microbial-based enzyme.

The above-mentioned " Carica papaya fruit " is a papaya fruit belonging to the throat neck of a dicotyledonous plant, and is originated from the tropical America and distributed in the tropical regions of the world. The papaya fruit has a ball shape, an inverted egg shape, a long egg shape, and contains a large amount of papain, a protein protease that is effective in controlling vitamins A, B and C, carotenoids and exfoliants.

Specifically, the papain component smoothly removes unnecessary skin horny layer, normalizes the keratinization cycle, and is excellent in smooth and transparent skin texture management effect. In addition, nutrition and conditioning can be given to the skin to create a vigorous and healthy skin, control the oil content, and achieve pore-shrinking effect.

The above-mentioned "Pumpkin ( Cucurbitaceae )" is one year old belonging to the bacillus, and its origin is cultivated in various places in the world in South America. In Korea, the tropical american oriental pumpkin ( Cucurbita moschata , Cucurita maxima from South America, and Cucurbita pepo from northern Mexico and North America. In Korea, pumpkin has traditionally been regarded as a good food for people with weak stomach, recovery patients, and postpartum edema, and is effective for hypertension, diabetes, neuralgia, women's health, postpartum cooking, It is known.

The amber contains carbohydrates, proteins, fats, carbohydrates, ash, fiber, calcium, vitamins and the like, and in particular contains provitamin A and carotene which become vitamin A in the human body. In addition, the unique aroma of the pumpkin fat component of unsaturated fatty acids, not only show excellent effects in preventing adult diseases, but also contains essential amino acid lecithin to improve the brain.

There is a large amount of carotenoid-based compounds which are yellowish in old amber and used as additives of various processed foods. Such carotenoid-based pigments include pigment components such as carotene, lycopene, and lutein. The composition of carotenoids is mainly constituted of β-carotene and α-carotene, and constitutes 67 to 96% of the total carotenoid amount. In particular, β-carotene, which shows the effect of vitamin A, has been reported to have various pharmacological effects. Recently, carotene, which is thought to be a precursor of vitamin A, has been reported to prevent cancer.

BETAINE SALICYLATE is a raw material for synthesizing salicylic acid and betaine. It is superior in moisturizing effect to beta-hydroxy acid used for normal exfoliation purposes, It is significantly lower.

The above-mentioned " Spicule " is a composite material composed of calcium and silicate in the form of various fine needles collected from sponges inhabiting freshwater in Europe and Russia, and can be generally obtained in the form of powder .

The fine needle-like structure is a fine needle-shaped structure and penetrates the skin layer to form a micro channel, so that penetration and keratin separation effect of the applied cosmetic ingredient can be remarkably increased. In addition, the fine needle can promote skin regeneration of collagen which acts on an artificial wound healing, and increase the skin tissue temperature to increase bioactivity.

The fine needle is a harmless substance to the human body and is not particularly limited as long as it is a powdery material capable of stimulating the skin to form fine pores and dissolved or discharged in the body.

In one embodiment, the micropowder powder can be obtained from a spongy animal, wherein the spongy animal is a Spongilla lacustris L. ), Spongilla fragile Leidy , and Ephydatia fluviatilis species.

The sponge animal is a primitive marine creature with no muscle, nerves or organs, and more than 5,000 species are present. The skeleton is a tissue that functions as a needle-like skeleton existing in the body of invertebrate animals, and mainly contains silicic acid and calcium carbonate. The sponge animal may comprise uniaxial, triaxial, tetragonal or multiaxial bone fragments containing calcium carbonate or silicic acid as a major component.

The sponge is sponge of various colors such as orange or cyan, and it lives mainly in the sea, but there is also a freshwater sponge.

The sponge animal is an organism that is in the initial stage of tissue in a state where cells are aggregated, and the body is composed of sponge fibers, spicules forming body wall, and cells bound to bone fragments. The main component of the bone fragments is calcite, silicic acid, silica or the like, and the size is about 100 탆 or so and can be observed by a microscope.

The bone fragments can be used as an effective means of skin regeneration to correct visual facial defects such as hyperpigmentation of various etiologies, fine wrinkles, sun damage, superficial scars, cotton wool, and enlarged pores. It is also being used as a natural alternative approach to microdermabrasion and skin erosion, chemical peeling, CO ₂ laser ablation, and erbium laser ablation, which are physical procedures to improve skin conditions.

When the bone fragments come into contact with and rub against the skin layer, they are physically separated to reduce keratinocyte aggregation, and the keratin layer, sebum plug, and keratinocytes can be desorbed to promote regeneration of the skin.

The average particle length of the fine needle-like powder may be 70 to 500 탆. If the average particle length is less than 70 탆, it can not effectively penetrate into the skin to form pores. If it is 500 탆, the dispersibility may be lowered or excessive stimulation to the skin may be caused.

The complex extract can exhibit an excellent exfoliation effect due to interaction between active ingredients derived from each raw material. The combined extract is applied to the skin together with various components contained in each extract, thereby increasing the absorption rate and maximizing the exfoliating effect.

The above-mentioned "extract" refers to a solvent in which an effective ingredient contained in an extraction raw material has been transferred by contacting a solvent and an extraction raw material under specific conditions. If a substance is separated from a natural product, the extraction method, You can include them all regardless. For example, it may include extracts of components dissolved in a solvent from natural products by using water or an organic solvent, and specific components of natural products, such as oils obtained by extracting only specific components such as oil.

The complex extract may be extracted with at least one solvent selected from the group consisting of purified water, a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol, It may be ethanol at 90% concentration (v / v).

The extraction ratio of the active ingredient contained in the raw material may be different depending on the polarity of the solvent. Since the ethanol is excellent in selectivity for extracting a physiologically active substance from a natural raw material, the ethanol extract can realize an optimal skin improving effect .

Particularly, the polarity of water and ethanol is different, so that the effective ingredient to be extracted according to each polarity can be changed, and the concentration of ethanol can be appropriately controlled so that an optimal skin improving effect can be realized. At this time, if the concentration of the ethanol exceeds 90%, an adequate yield may not be realized. If the concentration is less than 60%, the effective ingredient showing skin improving effect may not be extracted sufficiently.

The combined extract is washed with water, dried and pulverized, and then subjected to a conventional method such as circulation extraction, pressure extraction, and ultrasonic extraction for about 1 to 24 hours with a volume of 8 to 12 times the weight of the raw material Extraction and filtration. In addition, the extract can be obtained in powder form by an additional process such as vacuum distillation or freeze-drying.

The extract may also contain an extract which has been subjected to a conventional purification process. For example, the extract may be subjected to various purification methods such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity) Lt; RTI ID = 0.0 > fractions. ≪ / RTI >

The cosmetic composition may contain 0.5 to 40% by weight of the complex extract and 0.1 to 5% by weight of the micropowder powder based on the total weight of the composition. If the complex extract is less than 0.5% by weight, the keratin detachment effect by the complex extract may not be realized properly. If it is more than 40% by weight, the specific gravity of the other additives required for commercialization may decrease, have. If the fine needle powder is less than 0.1% by weight, the exfoliating effect of the keratin may be deteriorated. If the amount of the fine needle powder is more than 5% by weight, excessive skin irritation may be caused.

Meanwhile, the complex extract may contain 10 to 30 parts by weight of the Mucor miehei extract, 10 to 30 parts by weight of the papaya fruit extract, and 10 to 30 parts by weight of the pumpkin extract.

When the content difference between the active ingredients constituting the complex extract is out of the above range, the synergistic effect due to the interaction between the active ingredients may not be properly realized. Therefore, The ratio can be appropriately controlled.

On the other hand, the combined extracts may be fermented extract obtained by inoculating the Lactobacillus genus (Lactobacillus sp.) Strains.

The term " fermentation " refers to a process in which a microorganism decomposes an organic substance using an enzyme contained therein. It is known that a raw material that has undergone fermentation by the microorganisms has an effect of at least 2 times to a maximum of several tens of times greater than that before fermentation. Fermented metabolites contain various amino acids, organic acids and antioxidants that are good for the skin, promoting skin metabolism and making skin texture resilient and smooth. In addition, it has been reported that as the fermentation process proceeds, the particles become smaller and the toxicity such as heavy metals is decomposed and the absorption rate is improved, and the skin troubles and allergic side effects are alleviated.

The above-mentioned "fermentation extract" is obtained by drying the extract naturally or by using an apparatus, specifically a rotary vacuum concentrator and a freeze drier, diluting the extract or the resin extract to a certain concentration in a solvent, preferably purified water, For example, by inoculating and fermenting a microorganism, that is, a Lactobacillus sp . Strain.

The Lactobacillus genus strains Lactobacillus pento suspension (Lactobacillus pentosus), Lactobacillus brevis (Lactobacillus brevis), Lactobacillus Planta room (Lactobacillus plantarum), Lactobacillus casei (Lactobacillus casei) or Lactobacillus know also required Ruth (Lactobacillus acidophilus , but are not limited thereto.

The cosmetic composition may be at least one selected from the group consisting of softening lotion, astringent lotion, nutritional lotion, lotion, nutritional cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, .

The cosmetic composition may be formulated by a conventional method. The contents disclosed in Remington ' s Pharmaceutical Sciences, Mack Publishing Company, Easton PA in the formulation of the external preparation for skin can be referred to, and in the formulation of the cosmetic composition, International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association , Inc., Washington, 1995).

Specifically, the cosmetic composition may be prepared into a general emulsified formulation and a solubilized formulation. For example, lotion such as soft lotion or nutrition lotion; Lotion such as facial lotion, body lotion; Nourishing cream, moisture cream, cream such as eye cream; essence; Makeup ointment; spray; Gel; pack; Sunscreen; Makeup base; A foundation such as a liquid type, a solid type or a spray type; powder; Make-up removers such as cleansing creams, cleansing lotions and cleansing oils; Or a cleansing agent such as a cleansing foam, a soap, a body wash, and the like, but is not limited thereto. The external preparation for skin may be formulated into ointments, patches, gels, creams or sprays, but is not limited thereto.

The cosmetic composition of the present invention may be appropriately blended with other ingredients within the range of not compromising the object of the present invention depending on the kind of the formulation or the purpose of use, in addition to the essential ingredients in each formulation.

The cosmetic composition may contain a conventional acceptable carrier and may appropriately contain, for example, oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a colorant, no.

The acceptable carrier may vary according to the formulation. For example, when formulated into ointments, pastes, creams or gels, there may be used an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide, Mixture can be used

When the cosmetic composition is formulated into a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as a carrier component. In the case of a spray, Propellants such as carbon, propane, butane or dimethyl ether.

When the cosmetic composition is formulated into a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, 1,3-butyl glycol oil may be used, and in particular, fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan may be used.

When formulated into a suspension, the cosmetic composition may contain, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the cosmetic composition is formulated into soap, it is preferable to use, as a carrier component, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, Can be used.

The cosmetic composition may be a cosmetic or dermatological composition which is generally used in the art depending on the quality or function of the final product, such as a lipid, an organic solvent, a solubilizing agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, Surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, And may further contain adjuvants conventionally used in the field of cosmetics or dermatology, such as any other ingredient.

However, the adjuvant and the mixing ratio thereof can be appropriately selected so as not to affect the preferable properties of the cosmetic composition according to the present invention.

The present invention will be further described with reference to the following examples, but it should be apparent that the present invention is not limited by the following examples.

Manufacturing example  One: Mukor Miehei  Preparation of extract

To 10 g of Mucor fungus cultured on a medium, 0.1 ml of 75% ethanol and purified water were added and extracted three times repeatedly for 48 hours using ultrasonic waves. Thereafter, it was filtered using Watman Paper, and then dried at room temperature to obtain a solid component.

Manufacturing example  2: Preparation of papaya fruit and amber extract

Papaya fruit and pumpkin were washed with water, completely dried at room temperature and pulverized to obtain 100 g of pulverized product.

100 g of each pulverized product was immersed and extracted with a 10-fold volume of an aqueous ethanol solution (70% mass concentration) as a solvent at a temperature of 70 ° C for 12 hours.

The extract was filtered with 250 mesh and 0.5 mu m filters and then extracted three times in the same manner for the remaining raw materials. The extract was concentrated under reduced pressure at 30 DEG C and lyophilized to obtain a solid fraction.

Manufacturing example  3: Fine needle  Powder manufacturing

Dried sponges ( Spongilla 800 g of a 0.5 N sodium hydroxide solution was added to 200 g of the natural sponge obtained in lacustris and mixed and pulverized by an ultrasonic process. After stirring, impurities were removed by centrifugation and 700 ml of purified water was added to remove soluble impurities and sodium hydroxide solution.

A small amount of sodium hydroxide was removed by chemical reaction with 800 ml of a 2% -L-ascorbic acid solution, and then the charged L-ascorbic acid solution was removed, followed by drying to obtain fine needle powder from which purified water was removed.

Manufacturing example  4: Preparation of fermented extract

Each of the extracts obtained in Preparation Examples 1 and 2 was diluted to 5% by weight with purified water and then inoculated with a fermentation strain, Lactobacillus plantarum, and fermented for 12 hours.

The fermented extract was filtered through 500 mesh and 0.2 mu m filters to obtain a mucormierhe fermentation extract, a papaya fruit fermentation extract and an amber fermentation extract

Example  And Comparative Example

To examine the skin improving effect of the obtained extract and the fermented extract, examples and comparative examples were set by mixing samples as shown in Table 1 below.

Betaine salicylate was commercially available.

As shown in Table 1, the raw materials were mixed at a predetermined ratio or the peel-off effect of the micropowder powder according to the average particle length was examined.

Examples 1 and 2 and Comparative Examples 1 and 2 contained 100 탆, Examples 3 and 4 and Comparative Examples 3 and 4 were 400 탆, Comparative Example 5 was 20 탆, and Comparative Example 6 was 800 탆. .

[Content (parts by weight)] division Example
One Example
2 Example
3 Example
4 Comparative Example
One Comparative Example
2 Comparative Example
3 Comparative Example
4 Comparative Example
5 Comparative Example
6 Betaine salicylate 60 60 60 60 300 150 - - 60 60 Fine needle powder 60 60 60 60 - 150 - - 60 60 Mucormihei extract 60 - 60 - - - 100 - 60 - Papaya fruit extract 60 - 60 - - - 100 - 60 - Amber extract 60 - 60 - - - 100 - 60 - Mukommehei
Fermented extract - 60 - 60 - - - 100 - 60 Papaya fruit fermented extract - 60 - 60 - - - 100 - 60 pumpkin
Fermented extract - 60 - 60 - - - 100 - 60

Experimental Example  1: Skin safety test

MTT assay experiments were performed on fibroblasts (HDF), melanocytes (B16F10), and keratinocytes (HaCaT) to verify skin safety for the samples of the Examples and Comparative Examples.

The samples of Examples and Comparative Examples were each suspended in purified water at a concentration (50 mg / L), and cell viability was measured.

Cytotoxicity was performed by modifying the method of Mosmann to measure cell viability using MTT {3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide} reagent.

(HDF), melanocyte-forming cells (B16F10), and keratinocytes (HaCaT) were dispensed in 96-well plates at a concentration of 1 × 10 ⁴ cells / well and cultured at 37 ° C. and 5% CO ₂ for 48 hours Respectively.

After the culture medium was removed and the sample was cultured for 48 hours in a concentration-treated medium, the medium was removed and repeatedly washed with PBS (phosphate buffered saline). MTT was dissolved in PBS at 5 mg / mL and 50 L was added. The cells were cultured at 37 ° C and 5% CO ₂ for 24 hours. 100 L of DMSO (dimethyl sulfoxide) was added to each well, stirred for 10 minutes, and absorbance was measured at 540 nm.

As a result of measurement, cytotoxicity was not confirmed at all concentrations of each sample. The above results suggest that the sample of the composition is not only harmless to human body but also has excellent human safety.

Experimental Example  2: Promoting effect of keratinocyte differentiation

Human keratinocytes were cultured in a culture flask and grown to about 80%. The samples of the examples and comparative examples were added at the concentrations of 200 ppm and treated for 4 days as shown in Table 2 below.

SDS (sodium dodecyl sulfate) as a denaturant and β-mercaptoethanol as a reducing agent were mixed to remove dissolved proteins.

The concentration of keratinocytes was measured by measuring the absorbance of the remaining stratum corneum at 310 ㎚. The amount of keratinocyte was compared with the negative control (0%). At this time, TGF-beta (10 nM), a cell signaling substance, was treated as a positive control.

division Promoting effect of keratinocyte differentiation (%) Example 1 130.5 Example 2 142.9 Example 3 145.7 Example 4 153.7 Comparative Example 1 19.4 Comparative Example 2 22.7 Comparative Example 3 67.4 Comparative Example 4 72.6 Comparative Example 5 109.6 Comparative Example 6 102.7 Positive control group 142.8

The results are shown in Table 2. Referring to Table 2, the samples of Examples 1 to 4 were excellent in promoting keratinocyte differentiation, and the samples of Examples 2 and 4 containing fermented extracts showed remarkably improved keratinocyte differentiation promoting effect. In addition, the sample containing fine needle powder having a large particle average length within the effective range was excellent in promoting keratinocyte differentiation promotion.

In particular, the sample of Example 4 exhibited remarkably improved keratinocyte differentiation promoting effect as compared with TGF-beta, a cell signaling substance.

Experimental Example  3: skin moisturizing effect test

The skin moisturizing power improving effect of the cosmetic compositions containing the samples of Examples and Comparative Examples was evaluated. A cream form cosmetic composition of the same composition was prepared using the samples of Examples and Comparative Examples. Each sample was formulated into a cream of the same composition, except that the samples of Examples 1 to 4 and Comparative Examples 1 to 6 were different.

In order to measure the skin moisturizing effect of the cream form cosmetic composition, 100 subjects (20 to 40 year old female) were divided into 10 groups of 10 persons, and samples of the examples and comparative examples were included in the entire face for each group Was applied twice a day for 1 month continuously.

The skin conductivity was measured in advance using a corneometer (CM820 courage Khazaka electronic GmbH, Germany) under the constant temperature and humidity conditions (24 ° C, 40% humidity) before the start of application and the values were measured at 1 week, 2 weeks, The skin conductivity increase rate (%) was measured and evaluated. The results are shown in Table 3 below.

division 1 week 2 weeks 4 weeks Example 1 32 41 55 Example 2 39 51 62 Example 3 38 54 63 Example 4 49 65 72 Comparative Example 1 7 15 17 Comparative Example 2 11 13 19 Comparative Example 3 16 18 21 Comparative Example 4 19 20 26 Comparative Example 5 22 26 29 Comparative Example 6 25 27 31

Referring to Table 3, the experimental group to which the cosmetic composition of Examples 1 to 4 was applied exhibited an excellent skin conductivity increase rate as compared with Comparative Examples 1 to 4. In particular, the cosmetic compositions of Examples 2 and 4 containing the fermentation extract had an excellent effect of increasing the skin conductivity, and the effect of increasing the skin conductivity of the cosmetic composition containing the fine needle powder having an average particle size within the effective range Very good.

From the above results, it was confirmed that the cosmetic compositions containing the samples of Examples 1 to 4 kept the skin moisture content higher than the cosmetic compositions containing the samples of the comparative examples, because the skin conductivity was in proportion to the skin moisture content.

Experimental Example  4: Skin elasticity improvement effect test

A cream form cosmetic composition of the same composition was prepared using the samples of Examples and Comparative Examples. Each of the above samples was formulated into cream of the same composition, but the samples of Examples 1 to 4 and Comparative Examples 1 to 6 were different.

In order to measure the skin elasticity improvement effect of the cream form cosmetic composition, 100 subjects (20 to 40 years old) were divided into 10 groups of 10 persons, and cream containing a sample of each group and a comparative sample was divided into 10 groups For 2 consecutive months.

In order to confirm the improvement of skin elasticity, skin elasticity was measured before use and 2 months after using the skin elasticity meter (SEM 575, C + K Electronic Co., Germany). The experimental results are reported as the R7 value of Cutometer SEM 575, and the average value of each group is shown in Table 4 below. The R7 value indicates the nature of the viscoelasticity of the skin (n = 10, p < 0.05).

division R7 Example 1 0.32 Example 2 0.41 Example 3 0.43 Example 4 0.57 Comparative Example 1 0.10 Comparative Example 2 0.13 Comparative Example 3 0.21 Comparative Example 4 0.25 Comparative Example 5 0.26 Comparative Example 6 0.25

Referring to Table 4, the samples of Examples 1 to 4 effectively improved skin elasticity in the facial area.

In particular, when treating the cosmetic composition of Example 4 containing a fermented extract and a fine needle powder having an average particle size within a range, the elasticity of the facial region was remarkably improved.

Experimental Example  5: Evaluation of skin irritation

A skin patch test was conducted to examine the skin irritation test for each of the cream form cosmetic compositions. The subjects were selected as persons without skin disease at the test site of 15 to 30 years old.

200 subjects were divided into 10 groups of 20 persons each. The upper part of the pellet was wiped with 70% ethanol and dried. Then, 0.1 g of the product was applied for 24 hours, the skin reaction was checked within 1 hour after removal of the patch, and the next day (48 hours later). Skin response determinations were made according to the following test criteria. The skin patch test results are shown in Table 5.

division Judgment result - ± + ++ +++ Example 1 14 2 One 2 One Example 2 16 2 - One One Example 3 17 - 2 One - Example 4 18 2 - - - Comparative Example 1 2 5 5 4 4 Comparative Example 2 4 5 6 4 One Comparative Example 3 5 4 6 2 3 Comparative Example 4 5 6 4 4 One Comparative Example 5 7 4 2 3 4 Comparative Example 6 9 4 5 2 -

* Skin response test criteria / -: No reaction / ±: weak positive (erythema) / +: positive (erythema) / ++: strong positive (erythema, edema) / +++: severe positive , Swelling, blistering)

Referring to Table 5, the cream type cosmetics formulated with the samples of Examples 1 to 4 hardly induced skin irritation as compared with Comparative Examples 1 to 6. [

Experimental Example  6: Sensuality  evaluation

The cream form cosmetic composition was subjected to sensory evaluation.

The cream was applied to the right hand and the left hand of a total of 100 persons, 10 persons for each ages from 20s to 40s. The cream was applied to each of the hands and massaged to adjust the gel cohesion, skin irritation, exfoliation degree, .

 The highest score is 10 points, the average score is 5 points, and the score is 0 points. The score below is the average of 100 responses.

The results of sensory evaluation according to the above Test Example are shown in Table 6 below.

Evaluation items Gel cohesiveness Degree of exfoliation Skin irritation Skin moisturizing power Overall likelihood Example 1 7 7 8 6 7 Example 2 8 8 9 8 8 Example 3 9 8 8 9 8 Example 4 9 9 9 9 9 Comparative Example 1 4 3 6 5 2 Comparative Example 2 5 5 6 6 3 Comparative Example 3 4 6 5 5 6 Comparative Example 4 4 5 5 5 5 Comparative Example 5 5 6 2 6 5 Comparative Example 6 6 7 3 6 6

Referring to Table 6, it was evaluated that the cream type cosmetics formulated with the samples of Examples 1 to 4 were significantly superior in overall acceptability as compared with Comparative Examples 1 to 6.

In particular, all of the evaluated items, such as gel cohesion, exfoliation effect, skin irritation, and moisturizing ability, were evaluated to be high.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (9)

10 to 30 parts by weight of an extract of Mucor miehei, 10 to 30 parts by weight of a papaya fruit extract, and 10 to 30 parts by weight of an amber extract;
BETAINE SALICYLATE, and fine needle powder as an active ingredient. The method according to claim 1,
The fine needle powder is obtained from a sponge animal. 3. The method of claim 2,
The sponge animal is a Spongilla &lt; RTI ID = 0.0 &gt; lacustris . The method according to claim 1,
Wherein an average particle length of the fine needle-like powder is 70 to 500 占 퐉. The method according to claim 1,
The combined extract was fermented extract obtained by inoculating the Lactobacillus genus (Lactobacillus sp.) Strains of the cosmetic composition. The method according to claim 1,
Wherein the composite extract is contained in an amount of 0.5 to 40% by weight based on the total weight of the composition, and the fine needle powder is contained in an amount of 0.1 to 5% by weight. delete The method according to claim 1,
Wherein the complex extract is extracted with at least one solvent selected from the group consisting of purified water, a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol. The method according to any one of claims 1 to 6 and 8,
A cosmetic composition formulated with at least one selected from the group consisting of softening lotion, astringent lotion, nutritional lotion, lotion, nutritional cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, .