KR101823673B1 - Sleep inducing composition with nipa fruticans wurmb - Google Patents

Abstract

The present invention relates to a sleep inducing composition using stalks of Nypa fruticans Wurmb, and more specifically, the sleep inducing composition is a pharmaceutical or food composition containing an extract of stalks of Nypa fruticans Wurmb, an Ecklonia cava extract a rhubarb root extract, and Toddypalm Jaggery as effective components. The sleep inducing composition according to the present invention has an excellent sleep inducing function, does not cause side effects as the composition is derived from natural ingredients, and can secure safety.

Description

TECHNICAL FIELD [0001] The present invention relates to a sleep inducing composition using a nipponia palm flower. [0002] SLEEP INDUCING COMPOSITION WITH NIPA FRUTICANS WURMB [0003]

The present invention relates to a composition for inducing sleeping using a nifa palm phalanx, and more particularly to a composition for inducing sleep induction comprising a nifel palm phloem extract, a phlegm extract, a wilted root extract, and a toffee palm.

In modern society, many people suffer from sleep disturbance due to stress, anxiety and irritability. According to statistics, about 15% of adults are known to need medication because of insomnia due to anxiety symptoms. Insomnia is caused by various factors such as stress, tension, and fear.

Therapeutic drugs for insomnia include benzodiapine-based drugs such as quazepam, flurazepam, and triazolam, and zolpidem. The disadvantages of these drugs are that they can cause symptoms similar to anxiety, excitement and withdrawal, which are not only difficulties in daily life but also cause other diseases such as cognitive impairment or depression.

Therefore, there is an urgent need to develop a therapeutic agent for sleep disorders that replaces the above-mentioned drugs and does not show a risk factor.

KR 10-1545843 B1 KR 10-1260696 B1 KR 10-1260697 B1

Accordingly, it is an object of the present invention to provide a pharmaceutical composition for inducing sleep without any side effects such as cognitive impairment or depression.

It is also an object of the present invention to provide a food composition for inducing sleep which is free from adverse effects such as cognitive impairment or depression.

In order to accomplish the above object, the present invention provides a composition for inducing sleep using a nifa palm phlox, which comprises a nifel palm phlox extract, a phlegm extract, a horseradish root extract and a toffee palm as an active ingredient, and is a pharmaceutical composition do.

In addition, it is a food composition, comprising as an active ingredient, an extract of Nephaga palestris, a sensory extract, a horseradish root extract and a toffee palm extract.

In this case, the present invention is characterized in that the distillation product comprises the above-mentioned extracts of Nephagae, Lilium, Liliaceae and Todipam in a weight ratio of 4: 0.5-1.5: 0.5-1.5: 3-5.

The above-mentioned extract is obtained by adding a dry weight of 3 to 20 times by weight of a solvent to the dried powder of a nifa palm phlox and extracting it at an extraction temperature of 20 to 100 ° C for 30 minutes to 5 days. By weight of a dry weight of a solvent in an amount of 3 to 20 times by weight of a solvent and extracting the mixture at an extraction temperature of 20 to 100 ° C for 30 minutes to 5 days. By weight of a solvent in an amount of 3 to 20 times by weight and extraction at an extraction temperature of 20 to 100 DEG C for 30 minutes to 5 days.

The solvent is characterized by being water, ethanol or a mixed solvent thereof.

INDUSTRIAL APPLICABILITY The water-surface-inducing composition according to the present invention is advantageous in that it has excellent water-surface-inducing function and uses a substance obtained from a natural material, so that there is no side effect and safety can be ensured.

Hereinafter, the present invention will be described in detail.

It should be understood, however, that the invention is not intended to be limited to the particular embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

The water-insoluble composition of the present invention is composed of only natural substances and is characterized not only in that it has an excellent water-inducing effect but also has no side effects and can secure stability.

Specifically, the water-surface-inducing composition of the present invention contains the extracts of Nephlophyllum phloxus, Phloem phloem, Phlox root extract and Todipam as an active ingredient.

The above-mentioned NIPA FRUTICANS WURMB is originated in India, Malaysia, Southeast Asia and Australia, and grows in wetlands such as mangroves. The roots are divided into several pieces in the ground, and the leaves are gathered on the ground and have a glossy green color. And the flower is one male and one female, and it comes out from the axilla of the ground. Usually, the flower stands rise from October to June of the following year.

The reason for using the flower bud of Nipaya is that it has abundant antioxidant components such as polyphenols in the flower bud than any other part of Nipaya, This is because the content of phlorotannin is high. The fluorotannine has affinity for the GABA _A - benzodiazepine receptor and promotes sleep induction. That is, GABA _A Gamma-aminobutyric acid (GABA) acts on calming, sleeping, anxiety, muscle tension, convulsions, memory loss and the like, which is a pterameric protein that forms a membrane ion channel. Many drugs, including benzodiazepines as sleep inducing agents, bind to these receptors and perform pharmacological actions. When a drug or adjuvant is bound to the benzodiazepine site, the affinity of the GABA _A receptor for GABA is enhanced, And the effect of inducing and improving sleep is increased by increasing the infusion rate. The fluorotannine has a high affinity to the GABA _A - benzodiazepine receptor and thus has a great effect on induction and improvement of sleep. Therefore, the nifeliana extract containing fluorotannine is useful as a water-surface-inducing composition.

In the present invention, the extract of the nifa palm phlox is extracted from the dried powder of the nifa palm phalaenopsis.

At this time, the dried powder of the nihon paladin can include not only a nippae flower, but also a flower bud or a flower attached to a flower bed, and the nipponia palm is picked, dried and crushed. Here, the drying may be carried out in a known manner to such an extent that the useful components of the harvested napa are not destroyed. For example, the drying may be carried out by natural drying in the shade. In addition, the crushing is sufficient for crushing the crust to such an extent that the useful components of the nipponophyllum peduncle can be sufficiently extracted in the subsequent extraction process. In addition, the drying and crushing processes may be carried out in reverse order or repeatedly as required.

The extraction may be, for example, hot water extraction, cold extraction, warming extraction, reflux cooling extraction, ultrasonic extraction, or the like.

In addition, the extraction is carried out using a solvent, and water, ethanol, or a mixed solvent thereof may be used as the solvent.

The extraction is carried out by adding 3 to 20 times by weight of the dry weight of the dried powder to the nifa palm phlox, and more preferably at 30 to 5 days at an extraction temperature of 20 to 100 ° C, more preferably 40 to 90 ° C A method of extracting for 1 to 24 hours can be applied.

At this time, the liquid extract may be separated from the dried powder of the nifalia phalaenopsis by a method such as filtration, followed by concentration or drying. For example, the extract of the liquid phase may be a concentrate obtained by concentrating the extract at a reduced pressure at 20 to 100 ° C, preferably 40 to 70 ° C by using a vacuum rotary condenser, and drying the extract to obtain a powdered powdery extract. The extracted or concentrated extract may be used in water, ethanol or a mixed solvent thereof if necessary.

It is a marine algae with seaweeds in the seaweeds of the algae, and contains a large amount of phlorotannin having a water-inducing function as described above.

In the present invention, the extract of the menthol is extracted from the menthol dried powder. Here, the drying of the menthol may be carried out by a known method insofar as the useful components of the menthol are not destroyed, and may be carried out, for example, by natural drying in the shade. Further, the above-mentioned crushing is enough to crush to such an extent that the useful components of the crushing can be sufficiently extracted in the subsequent extraction process. In addition, the drying and crushing processes may be carried out in reverse order or repeatedly as required.

The extraction may be performed by hot water extraction, cold extraction, warm-up extraction, reflux cooling extraction, ultrasonic extraction, or the like, and water, ethanol, or a mixed solvent thereof may be used, .

The extraction is carried out at a temperature of 20 to 100 ° C, more preferably at an extraction temperature of 40 to 90 ° C for 30 minutes to 5 days, more preferably 1 For 24 hours may be applied.

In addition, the liquid extract may be separated from the dried powder by filtration or the like, and then subjected to a concentration or drying process. For example, the extract of the liquid phase may be a concentrate obtained by concentrating the extract at a reduced pressure at 20 to 100 ° C, preferably 40 to 70 ° C by using a vacuum rotary condenser, and drying the extract to obtain a powdered powdery extract. The extracted or concentrated extract may be used in water, ethanol or a mixed solvent thereof if necessary.

In addition, the root of the buttercup is dried by the root of the buttercup, and it has a strong bitter taste. The wooly root is rich in polyphenol, choline, dietary fiber, etc., and is effective for the removal of intestinal toxins, and is effective for bronchial diseases, vascular diseases, headache alleviation, and induces a good sleep.

In the present invention, the extract of the wooly root is extracted from the dried powder of the wooly root. Here, the drying of the wooly roots may be carried out by a known method to such an extent that the useful components of the rosacea are not destroyed, and may be carried out, for example, by natural drying in the shade. Further, the above-mentioned crushing is enough to crush to such an extent that the useful components of the crushing can be sufficiently extracted in the subsequent extraction process. In addition, the drying and crushing processes may be carried out in reverse order or repeatedly as required.

The extraction may be performed by hot water extraction, cold extraction, warm-up extraction, reflux cooling extraction, ultrasonic extraction, or the like, and water, ethanol, or a mixed solvent thereof may be used, .

The extraction is carried out at a temperature of 20 to 100 ° C, more preferably at an extraction temperature of 40 to 90 ° C for 30 minutes to 5 days, A method of extracting for 1 to 24 hours can be applied.

In addition, the liquid extract may be separated from the dried powder of the hull root by a method such as filtration, and then subjected to a concentration or drying process. For example, the extract of the liquid phase may be a concentrate obtained by concentrating the extract at a reduced pressure at 20 to 100 ° C, preferably 40 to 70 ° C by using a vacuum rotary condenser, and drying the extract to obtain a powdered powdery extract. The extracted or concentrated extract may be used in water, ethanol or a mixed solvent thereof if necessary.

In addition, the jaggery of toddy palm has taken the sap of palm tree tofu (palm palm tree) and heated it to make a solid lump. Then, when water is added again and dissolved, it becomes a concentrated liquid. These toffee palms are excellent in flavor, but contain a large amount of vitamin E and selenium. Moreover, the distance of toddy palm also acts as a digestive agent, which helps induce sleep. In the present invention, the toffee palm distance can be obtained by purchasing commercially available products, and can be used in the form of a solid or a concentrate.

In the present invention, it is preferable that the distance of the niftha palm phloxus extract, moxibustion extract, horseradish root extract and toffee palm is in the range of 4: 0.5 ~ 1.5: 0.5 ~ 1.5: 3 ~ 5 weight ratio, This is because it has the best function.

The water-surface-inducing composition of the present invention can be used as a pharmaceutical composition.

In addition, such a pharmaceutical composition may further comprise additional components, for example, at least one of a carrier, an excipient, and a diluent, and more specifically, lactose, dextrose, sucrose, But are not limited to, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, Hydroxybenzoate, hydroxybenzoate, talc, magnesium stearate, and mineral oil.

The water-surface-inducing composition may have various formulations and may be formulated in the form of external preparations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups and aerosols according to a conventional method. In the case of formulation, it can be prepared using diluents or excipients such as fillers, extenders, binders, humectants, disintegrants, surfactants and the like which are usually used.

The preferred dosage of the water-insoluble composition can be appropriately selected depending on the condition and the weight of the patient, the degree of disease, the type of the drug, the administration route and the duration. However, for the desired sleep-inducing effect, the extract or the compound of the present invention can be administered in an amount of 50 to 200 mg / kg per day divided once to several times per day.

In addition, the water-insoluble composition according to the present invention can be used as a food composition, and includes an effective ingredient of the extract of Nephi palm phlox, phloem extract, horseradish extract, and toffee palm. Or as a food composition in the form of a food additive.

Food compositions of this type can be prepared in a variety of forms according to conventional methods. For example, the health food may be prepared by liquefying, granulating, encapsulating and pulverizing the extract in the form of tea, juice and drink so that it can be consumed. In addition, the composition can be prepared in the form of a composition by mixing together the above-mentioned Nihonbara phalaenopsis extract, phytate extract, horseradish extract and toffee palm as well as known active ingredients known to have a sleep inducing effect. Functional foods also include, but are not limited to, beverages (including alcoholic beverages), fruits and processed foods such as canned fruits, jars, jam, maare marlade, fish, meat and processed foods (Eg butter, cheeses, etc.), edible vegetable oils (eg, sourdoughs, etc.), breads and noodles (eg udon, buckwheat noodles, ramen noodles, spaghetti, macaroni, , Margarine, vegetable protein, retort food, frozen food, various kinds of seasonings (for example, soybean paste, soy sauce, sauce, etc.). The preferable content of the above-mentioned nifedium phalaenopsis extract in the food composition is not particularly limited, but is preferably 0.01 to 50% by weight in the finally prepared food.

Hereinafter, the present invention will be described in detail with reference to Examples, Experimental Examples and Preparation Examples. EXAMPLES The following Examples, Experimental Examples and Preparation Examples are merely illustrative of the present invention, and the contents of the present invention are not limited by the following Examples, Experimental Examples and Preparation Examples.

(Example 1)

The flower buds of Nipaya were picked, dried naturally on a well-ventilated shade, and then shredded to a size of 20 to 100 mesh to obtain a dried Nipana palm flower. Then, 1 L of water was added to 100 g of the above-mentioned dried niacin, and the mixture was extracted at 70 캜 for 10 hours, and then filtered with filter paper to obtain an extract. The solvent was then concentrated under reduced pressure to 70 Brix.

Dried persimmon was purchased and crushed to a size of 20-100 mesh. Then, 1 L of water was added to 100 g of the gentle-dried powder, and the mixture was extracted at 70 캜 for 10 hours, and then filtered with a filter paper to obtain an extract. The solvent was then concentrated under reduced pressure to 70 Brix.

Dried habitation roots were purchased and shredded to a size of 20-100 mesh. Then, 1 L of water was added to 100 g of the dried wort-ground powder, and the mixture was extracted at 70 캜 for 10 hours, and then filtered with filter paper to obtain an extract. The solvent was then concentrated under reduced pressure to 70 Brix.

100 g of the solid toffee palm distance was purchased, and 500 ml of water was added to dissolve it, and then concentrated to 70 Brix.

Then, the above-mentioned extracts of Nepharahia lobata, Rhizoma extract, wilted root extract, and toffee palm were mixed at a weight ratio of 4: 1: 1: 4.

(Test Example 1)

As test animals, 50 ICR male mice weighing 17 ± 1 g were divided into 5 groups. Breeding conditions were maintained at 22 ℃, 55% relative humidity, 11 times per hour of ventilation, 12 hours of light cycle, and all experimental animals were adapted for one week in the laboratory animal room. The animals were fed free of diet and water for 5 days at the same time once per day. Pentobarbital (30 mg / kg, ip) was injected 45 minutes after the oral administration of the final sample. sleeping time and sleep latency were measured. The elevation time was regarded as the elapsed time until the righting reflex was lost by 1 minute or more after peritoneal injection of pentobarbital, and the sleeping time was set to the time until the specular recovery was restored again.

Diazepam (DZP) was used as a positive control, and a 0.5% CMC-saline solution was treated at a concentration of 10 mg / kg as a general control group.

The results are shown in Tables 1 and 2 below.

The sleeping time measurement result of Test Example 1 division Administration sample Dosage Concentration (mg / kg) Sleep time (minutes) Growth rate (%) Test group Example 1 5 35 ± 5 112 Example 1 50 45 ± 6 145 Example 1 200 72 ± 7 205 Control group
Saline solution 10 31 ± 3 - Diazepam 2 75 ± 8 214

The elevation time measurement result of Test Example 1 division Administration sample Dosage Concentration (mg / kg) Elevation time (minutes) Decrease (%) Test group Example 1 5 7 ± 0.5 14 Example 1 50 6 ± 0.4 25 Example 1 200 5 ± 0.8 38 Control group
Saline solution 10 8 ± 1.0 - Diazepam 2 5 ± 0.6 38

As shown in Tables 1 and 2, in the mice to which Example 1 was administered, the effect of increasing the sleeping time and the effect of decreasing the surface time were shown in a concentration-dependent manner. In addition, at a concentration of 200 mmg / kg, it was confirmed that sleeping inducing effect was similar to that of the diazepam, which is a positive control group. Therefore, it was determined that the composition according to the present invention had a very excellent sleep inducing effect at a concentration of 50 to 200 mg / kg.

(Test Example 2)

The activity of inhibiting the binding reaction of ³ H-flumazenil to the receptor of the benzodiazepine receptor of Example 1, a selective antagonist, was specified.

The GABA _A - benzodiazepine receptor binding activity was measured as follows. The cerebral cortex of the mouse was immediately removed and homogenized in 20 ml of 30 mM Tris-HCl buffer (pH 7.4, 4 ° C) and sonicated for 10 seconds. After centrifugation at 27,000 × g and 4 ° C. for 15 minutes, the supernatant was discarded and centrifuged again with 20 mL of buffer. To remove GABA in the brain tissue, the cells were allowed to stand for 30 minutes in a 37 ° C water bath and centrifuged. The pellets were collected and stored at -80 ° C for freezing.

The frozen postsynaptic membrane was thawed and centrifuged at 27,000 × g and 4 ° C. for 10 min. The supernatant was discarded and centrifugation was repeated 3 times with 20 mL of 50 mM Tris-citrate buffer (pH 7.1, 4 ° C., binding buffer) The membrane was then suspended in 500 mL buffer / g original tissue in binding buffer and used for binding assays. 180 μL of the membrane suspension, 10 μL of each sample, and 10 μL of 1 nM ³ H-flumazenil were placed in a 96-well plate and allowed to react for 40 minutes in an ice bath. And then collected using a glass fiber filter (GF / C, Whatman). The radioactivity of the samples was measured with Tri-Carb Liquid Scintillation Analyzers (Perkin-Elmer, Shelton, CT, USA). The nonspecific binding (NSB) was determined using clonazepam (1 μM) and the binding displacement (%) was calculated using the following equation.

(1)

(DPM - NSB DPM) / (TB DPM - NSB DPM))] × 100 (binding displacement,%)

(DPM: disintegrations per minute, TB: total binding, NSB: nonspecific binding)

The results are shown in Table 3 below.

The results of Test Example 2 division Administration sample Dosage Concentration (mg / kg) Combined Movement (%) Test group Example 1 5 35 Example 1 50 51 Example 1 200 62

As shown in Table 3, when the composition of Example 1 was administered, it was confirmed that the binding activity of GABAA-benzodiazepine receptors replacing ³ H-flumazenil binding was increased.

(Preparation Example 1): A pharmaceutical preparation

(Production Example 1-1)

After the composition of Example 1 was dried and powdered, 2 g of the powder prepared in Example 1 and 1 g of lactose were mixed and filled in an airtight container to prepare a powder.

(Preparation Example 1-2)

After the composition of Example 1 was dried and pulverized, 100 mg of the powder prepared in Example 1, 100 mg of corn starch, 100 mg of lactose and 2 mg of magnesium stearate were mixed and then tableted according to a conventional preparation method. .

(Preparation Example 1-3)

After the composition of Example 1 was dried and powdered, 1 g of the powder prepared in Example 1, 1.5 g of lactose, 1 g of glycerin and 0.5 g of xylitol were mixed, and the mixture was prepared to be 4 g per one ring according to a conventional method Respectively.

(Production Example 2): Production of food

(Preparation Example 2-1)

0.5 g of sodium chloride and 200 ml of water were uniformly blended into 20 ml of the composition of Example 1 and sterilized instantaneously and then packaged in a glass bottle or PET bottle to prepare a health drink.

(Preparation Example 2-2)

100 ml of the composition of Example 1 was added to 1,000 ml of apple juice, and fruit juice was prepared by a conventional method.

Although the composition ratio is comparatively mixed with a component suitable for food as a preferred embodiment, the composition ratio may be arbitrarily modified according to the preference of the consumer.

Claims (6)

Wherein the composition is a pharmaceutical composition comprising, as an active ingredient, an extract of Nepharahia phalaenopsis, a phytase extract, a horseradish root extract and a toffee palm extract.
The method according to claim 1,
The composition of claim 1, wherein the composition comprises 4: 0.5 ~ 1.5: 0.5 ~ 1.5: 3 ~ 5 weight ratio of the extracts of Nephagosaccharomyces sp.
3. The method of claim 2,
The above-mentioned extract is obtained by adding a dry weight of 3 to 20 times by weight of a dry powder to a dried powder of a nifa coconut palm and extracting it at an extraction temperature of 20 to 100 DEG C for 30 minutes to 5 days.
The guttate extract is obtained by adding 3 to 20 times by weight of a dry weight of a dry powder to the ginger powder and extracting the ginger powder at an extraction temperature of 20 to 100 DEG C for 30 minutes to 5 days,
Wherein the wooly root extract is obtained by adding a dry weight of 3 to 20 times by weight of a solvent to the dried powder of wooly root and extracting at an extraction temperature of 20 to 100 ° C for 30 minutes to 5 days. A sleep inducing composition.
The method of claim 3,
Wherein the solvent is water, ethanol or a mixed solvent thereof.
Wherein the composition is a food composition, comprising the extract of Nephaga palmarifolia, a sensory extract, a horseradish root extract and a toffee palm as an active ingredient.
6. The method of claim 5,
The composition of claim 1, wherein the composition comprises 4: 0.5 ~ 1.5: 0.5 ~ 1.5: 3 ~ 5 weight ratio of the extracts of Nephagosaccharomyces sp.