KR101820761B1 - Composition for improving sleep disturbance and sleep inducing - Google Patents

Abstract

The present invention is based on the finding that the present invention can provide safety without causing side effects of synthetic drugs including phenobarbital, benzodiazepine, and the like, and has an affinity for GABAA-benzodiazepine receptor, Wherein the composition comprises 10 to 90 parts by weight of casein or casein hydrolyzate and 2 to 10 parts by weight of potassium chloride, wherein the composition comprises at least one member selected from the group consisting of a root extract, a hop extract, a lettuce extract And an extract of any one selected from the group consisting of a mixture of two or more thereof.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a composition for improving sleep disturbance and sleep inducing,

The present invention relates to a composition for improving sleep disturbance and a composition for inducing sleeping, and more particularly, to a composition for preventing sleep disorder, which is composed of constituents derived from a natural product and which does not cause side effects of a synthetic medicine including phenobarbital and benzodiazepine, And has an affinity for the GABAA-benzodiazepine receptor, thereby improving the sleeping disorder, and a composition for inducing sleeping.

Sleep disturbance is one of the most common problems of modern people. A sleeping disorder is a condition in which a person can not take a healthy sleep, is awake during the day despite having sufficient sleep, or has difficulty sleeping or awake when his sleep rhythm is disturbed. Mental problems are also a major cause, but they are caused by excessive stress, anxiety, tension, and fear. Sleep disorders are a very common disorder in which more than 20% of the population have experienced or are suffering. Sleep disturbances can cause a variety of personal and social problems and can be causes of learning disability, inefficiency, traffic accidents, safety accidents, emotional disturbances, social adjustment disorders, dissatisfaction with marriage, and industrial accidents. In addition, if the sleep disorder is not properly treated, the medical, neurological, and psychiatric illnesses that are already suffering may deteriorate or the recovery may be delayed, and serious diseases such as myocardial infarction and stroke may result. Therefore, modern people are increasingly interested in sleep aids.

Benzodiazepines and serotonergic drugs have been used as therapeutic drugs. Many drugs, including benzodiazepines, bind to the GABAA receptor and perform pharmacological actions. When a drug or adjuvant is bound to the benzodiazepine site, the GABAA receptor affinity for GABA is enhanced and the intracellular influx of the chloride ion is increased Anxiety relief, seizure improvement, sedation, and sleep induction and improvement. The GABAA receptor is a pentameric protein that forms a membrane ion channel and is closely related to the regulation of sedation, sleep, anxiety, muscle tension, convulsions, memory loss, etc. through which GABA (gamma-aminobutyric acid) acts. These drugs, when used for a long period of time, are accompanied by side effects that result in resistance and dependence, and may cause serious problems such as muscle relaxation and forgetfulness.

Therefore, the need for natural supplements is increasing as a means of replacing sleeping pills in the case of long-term sleepers and restrictors, and the demand for functional foods for sleep enhancement is increasing.

Korean Patent Registration No. 10-0947353 relates to a health food composition for improvement of sleeping disorder and a pleasant surface. Especially, it is a ring composed of a sanjoin, a tranquilizer, a livestock meat, a lotus root, and magnesium which are known to help the pleasant surface among natural products, and a melatonin content And a health food composition for improvement of sleeping disorder and a pleasant surface showing an excellent sleeping disorder improving effect.

Korean Patent Laid-Open Publication No. 10-2014-0030454 relates to a fermentation material for relieving stress or improving sleeping disturbance containing a natural ga bar by fermenting a mixture of oysters and seaweeds, and a method for producing the fermented material, wherein a mixture of oysters and seaweeds And fermenting the fermented material to improve stress-relieving or sleeping disorder containing a large amount of natural GABA, and a process for producing the same. The fermented material prepared by fermenting a mixture of oysters and sea tangle according to the present invention contains a large amount of taurine and GABA as compared with conventional fermented materials, and has been shown to be excellent in stress suppressing effect and sleeping disorder improving effect.

The present invention is based on the finding that the present invention can provide safety without causing side effects of synthetic drugs including phenobarbital, benzodiazepine, and the like, and has an affinity for GABAA-benzodiazepine receptor, And to provide a composition for improving sleep disorder and a composition for inducing sleeping which can be improved.

The sleep disorder improvement and sleep inducing composition according to the present invention comprises 10 to 90 parts by weight of casein or casein hydrolyzate and 2 to 10 parts by weight of potassium chloride.

The sleep-disorder improvement and sleep-inducing composition may further comprise 1 to 10 parts by weight of the Rhizome grass extract.

The above-mentioned Porphyra grass extract is obtained by immersing a dried pterygium outpost in a 10 to 70% ethanol aqueous solution of 15 to 25 times the total amount of the building, extracting it at a temperature within a range of 60 to 90 ° C for 2 to 8 hours, And the resulting extract may be concentrated and dried to be powdered.

The sleep disorder improvement and sleep inducing composition may further comprise 1 to 10 parts by weight of the hop extract or 1 to 30 parts by weight of the lettuce extract.

The hop extract is prepared by dipping the dried hopper in a 10 to 70% ethanol aqueous solution of 15 to 25 times the total amount of the building, extracting the mixture at a temperature within the range of 60 to 90 ° C for 2 to 8 hours, It may be concentrated and dried to be powdered.

The extract of lettuce is prepared by immersing the dried lettuce plant in a 10 to 70% aqueous solution of ethanol in an amount of 15 to 25 times the total amount of the building, extracting it at a temperature within the range of 60 to 90 ° C for 2 to 8 hours, May be concentrated and dried to be powdered.

According to the present invention, it is possible to secure safety without causing side effects of synthetic drugs including phenobarbital and benzodiazepine, which are composed of components derived from natural products, and have affinity to GABAA-benzodiazepine receptor, The present invention provides a composition for improving sleep disturbance and a composition for inducing a sleeping which can improve a sleep disorder, thereby providing a high affinity to the GABAA-benzodiazepine receptor, thereby improving sleep disorder, maintaining sleep maintenance time after taking, Can be reduced. In addition, by improving the quality of sleep, the satisfaction of daily life can be improved.

Hereinafter, the present invention will be described in detail with reference to specific examples.

The sleep disorder improvement and sleep inducing composition according to the present invention is characterized by comprising 10 to 90 parts by weight of casein or casein hydrolyzate and 2 to 10 parts by weight of potassium chloride.

In the present invention, it is possible to improve the sleeping disorder by using casein or casein hydrolyzate derived from milk as a raw material of food or a mixture of potassium chloride and milk, and it is also possible to provide a composition for preventing sleep disturbance, And the present invention has been accomplished. That is, based on a mixture of milk-derived casein and potassium chloride, which is effective for increasing blood pressure due to stress, decreasing the secretion of stress hormone, and emotional stability, the mixture is prepared by mixing the extracts of Porphyra vulgaris and / Thereby producing a natural product which has no side effects and improves the sleeping disorder and induces a sleep inducing effect.

Casein is a major protein of milk and is a protein of phosphorus and consists of αs-, β-, κ-casein and other trace amounts of casein. Milk-derived casein is known to help increase blood pressure, reduce stress hormone secretion, and maintain emotional stability. Tryptophan, which is contained in milk protein casein, is used as a source of 'serotonin' to control mood. Serotonin is converted into melatonin that regulates sleep in the body, stabilizing the nerves and inducing sleep. Thus, a child who is breastfeeding can sleep comfortably. According to one study in the United States, feeding newborns with formula containing tryptophan has a shorter time to sleep than the control newborn. Casein as a foodstuff is used in the production of mock oil, coffee creamer, processed cereal food, sausage, ice cream mix, casein plastic topping, etc. Industrial adhesives made of casein are used for woodworking, and casein plastic buttons are used for dyeing They are used for these purposes because of the convenience of gloss. Food additives include rennet casein and sodium caseinate (soda casein). Casein has emulsifying, thickening, and moisturizing functions, and is used in food processing using these functions. Its main uses are imitation cheese, coffee creamer and powder cream, powdered milk for children, bakery products, processed fish products, processed meat products, spaghetti and mayonnaise.

The casein hydrolyzate is made by heat-treating casein, a milk protein, and enzymatically digesting it with pancreatic trypsin. Phe-Phe-Ala-Pro-Glu-Val-Phe-Gly-Lys] peptides composed of 12 amino acids and standardized as 5-6% content can be commercially purchased and used. The casein hydrolyzate is proposed to lower the blood pressure by lowering the activity of angiotensin I converting enzyme in the body. When the casein hydrolyzate was ingested in hypertensive animals, blood pressure was significantly reduced. In a human application study comparing the supplementation effects of casein hydrolysates for adults who are worried about high blood pressure (systolic blood pressure lower than 200 and diastolic blood pressure lower than 109), supplementation of casein hydrolyzate may help lower blood pressure . The results of the ensured data are consistent, but the functional grade is equivalent to "Other Function II" due to insufficient number of studies in the base and human applications.

Potassium chloride is a compound consisting of potassium and chlorine ionic bonds. Potassium in potassium chloride is known to lower blood pressure and induce sedation and sleep. Potassium is an ion (K ⁺ ) in our body that exists in large amounts in the inner fluid of cells and is involved in the regulation of muscle and nerve function. Recent studies have shown that potassium channels in nerve cells play an important role in generating sleep in the brain during deep sleep in mammals. Chloride ion in potassium chloride is known to play an important role in sleeping. When chlorine ions enter through the ion channel, the potential inside the cell becomes more negative and becomes sedentary. The sodium content of common salt of edible salt is 88%, which can cause hypertension, heart disease, stroke, etc. In countries where salt is consumed as much as in our country these days, potassium chloride is added while maintaining salty taste of salt, Sodium salt, which is reduced by 28 to 60%, is gaining popularity. Sodium (Na) attracts water and increases blood pressure, but potassium (K) has the effect of lowering blood pressure by discharging sodium (Na) in urine to the body. However, excessive intake of potassium can lead to increased potassium levels in the blood, resulting in difficulty in breathing, chest pain, heart attack, etc., which can be very dangerous. In particular, medicines taken by patients with kidney disease are prescribed potassium-rich medicines, which can lead to fatal risks, so you should consult your doctor and use low sodium salt.

The specific mixing ratio of "10 to 90 parts by weight of casein or casein hydrolyzate and 2 to 10 parts by weight of potassium chloride" was selected as the optimum mixing ratio for improvement of sleeping disorder and induction of sleep through concrete experiments of the present inventors.

The sleep-disorder improvement and sleep-inducing composition may further comprise 1 to 10 parts by weight of the Rhizome grass extract.

It is a perennial plant of the perennial plant of the dicotyledonous plant. The scientific name is Valeriana fauriei . It grows in a somewhat humid place of mountain. The roots growing in the ground stretch as they grow to the side, and their roots are the roots of beard and have a unique smell similar to the smell of rat piss. She eats young seeds as herbs. Root leavening in the juvenile grass has effects on the condition, headache and pain. One study found that patients with mood swings had a sedative effect and were also effective in relieving fatigue by stimulating. It is an excellent treatment for relieving anxiety, nervousness and insomnia. It also has a radiant effect that calms the throbbing. Several studies have shown that it has the effect of lowering blood pressure. Barreport Liam has an anticonvulsant action, so it has an excellent effect on neurogenic dyspepsia, stomach cramps and irritable gastritis. Use tea as a skin cleanser to soothe skin inflammation. Essential oils are also used as cosmetics and fragrances for tobacco. In Oriental medicine, roots are used as medicines, which are effective in mental anxiety, nervous breakdown, myocarditis, postpartum heart disease, heart failure, menstrual irregularity, stomach cramps, arthritis and bruises. Gwangneung that hairy fruit valerian (var. Dasycarpa), and on the edge of the cracked pieces of leaves as ginip valerian (var. Integra) that does not have teeth. In Europe, the roots of var. Officinalis have been used as diuretics, analgesics, and tongue economics since BC, and are now used in hysteria and neurosis. The administration of large doses of the barley grass paralyzes the central nervous system and strengthens the cerebral cortex inhibition process, weakening reflexes and excitability, and releasing the smooth muscle spasm. Pharmacological actions that exert blood pressure in association with parasympathetic stimulation and inhibition of the central nervous system; And pharmacological actions that block antiviral and viruses that cause immune deficiency and have some antidiuretic action.

The above-mentioned Porphyra grass extract is obtained by immersing a dried pterygium outpost in a 10 to 70% ethanol aqueous solution of 15 to 25 times the total amount of the building, extracting it at a temperature within a range of 60 to 90 ° C for 2 to 8 hours, And the resulting extract may be concentrated and dried to be powdered.

The sleep disorder improvement and sleep inducing composition may further comprise 1 to 10 parts by weight of the hop extract or 1 to 30 parts by weight of the lettuce extract.

Hope is a multi-year-old chronic herbaceous Humulus lupulus of European origin belonging to the genus Phalaenopsis . The male predominantly uses unmodified female flowers of beech. Hope has a nerve sedative effect and sleep inducing effect, which helps to treat anxiety and insomnia. It acts like melatonin to lower body temperature and promote sleep.

The hop extract is prepared by dipping the dried hopper in a 10 to 70% ethanol aqueous solution of 15 to 25 times the total amount of the building, extracting the mixture at a temperature within the range of 60 to 90 ° C for 2 to 8 hours, It may be concentrated and dried to be powdered.

Lettuce is an annual plant of dicotyledonous plants. The scientific name is Lactuca It is sativa . It is native to Europe and West Asia and is widely grown as a vegetable. The trunk is divided into many branches, the height is 90 to 120 cm, and there are no hairs in the whole. There are many varieties, and the leaves are edible before the stem of the flower emerges. Bitter sesquiterpene lactones (BSL), a bitter component of lettuce, has been shown to have sedative and sleep effects. In one room, whole plants and seeds are used as medicines except for roots. Plants are effective when there is insufficient blood in urine and mother's milk. Seeds are watered and dalyeose when hypertension and mother's milk are insufficient. Lettuce has a very long history of cultivation and was recorded as a crop on ancient Egyptian pyramid murals dating back to about 4500 BCE. It was also recorded as a table of Persian kings in 550 BC, and grew as a significant vegetable in Greek and Roman times. In China, it first appeared in the Tang Dynasty, the 713th year of the year. In Korea, the date is not certain, but it was introduced through China. Currently, there is grown in large areas, such as South Korea, China, Japan, USA, UK, grown lettuce greatly envoys are varieties differentiation lot of lettuce (var. Capitata), ipsangchu (var. Crispa), cabbage lettuce (var. Longifolia) , And stem lettuce ( var. Asparagina ). Lettuce is mainly used for wrapping salads and wrappers, and it also eats roots. It is rich in vitamins and minerals, and is good for anemic patients. Lactucerin and lactic acid are contained in the juvenile juice from the stalk. It has analgesic and hypnotic effects.

The extract of lettuce is prepared by immersing the dried lettuce plant in a 10 to 70% aqueous solution of ethanol in an amount of 15 to 25 times the total amount of the building, extracting it at a temperature within the range of 60 to 90 ° C for 2 to 8 hours, May be concentrated and dried to be powdered.

The composition according to the present invention as described above may contain, as a main component, the above-mentioned components and a combination of a pharmaceutically and pharmaceutically acceptable carrier such as vitamins as an additional ingredient.

The above-described composition according to the present invention may further contain one or more active ingredients showing the same or similar functions in addition to the above components.

Hereinafter, preferred embodiments and comparative examples of the present invention will be described.

The following examples are intended to illustrate the invention and should not be construed as limiting the scope of the invention.

Manufacturing example  1 to 6

Each of these plant buildings was harvested from the building using the dried persimmon grass, hops and lettuce as shown in Table 1 below. Was immersed in 20% ethanol (alcohol alcohol) and 60% ethanol (alcohol alcohol) in an amount of 20 times the total amount and extracted at 80 DEG C for 5 hours. After filtration through Watman filter paper, the extract was concentrated under reduced pressure at 60 ° C and dried to give a powder.

Plant to be extracted Extraction solvent Production Example 1 Spiny grass 20% alcohol Production Example 2 Hope Production Example 3 Lettuce Production Example 4 Spiny grass 60% alcohol Production Example 5 Hope Production Example 6 Lettuce

Example  1 to 4 and Comparative Example  1 to 3

Casein, potassium chloride and the extracts obtained in the above Preparation Examples 1 to 6 were used to prepare a composition for improving sleep disturbance and a sleeping composition by mixing (parts by weight) in the proportions shown in the following Table 2 and used in the following experiments.

Kinds Comparative Example Example One 2 3 One 2 3 4 5 6 7 Casein 40 - - 40 40 40 40 40 40 40 Potassium chloride - 5 - 5 5 5 5 5 5 5 Swallow grass extract
(Production Example 1) - - 4 - 4 3 4 5 4 4 Hop extract
(Production Example 2) - - One - - One One One One - Lettuce extract
(Production Example 3) - - 3 - - 3 3 3 - 3

Experimental Example  One: GABAA - Benzodiazepine  Detection of receptor binding activity

The GABAA-benzodiazepine receptor binding activity was measured by homogenizing the mouse cerebral cortex and immediately adding it to 20 ml of 30 mM Tris-HCl buffer (pH 7.4, 4 ° C) and sonication for 10 seconds Respectively. After centrifugation at 27,000 × g and 4 ° C. for 15 minutes, the supernatant was discarded and centrifuged again with 20 ml of buffer. This procedure was repeated three times. To remove GABA in the brain tissue, the cells were allowed to stand in a 37 ° C water bath for 30 minutes and centrifuged. The pellets were collected and stored at -80 ° C for freezing. The frozen membranes were thawed and centrifuged at 27,000 × g and 4 ° C. for 10 min. The supernatant was discarded and 20 ml of 90 mM Tris-citrate buffer (binding buffer, pH 7.1, 4 ° C.) After the centrifugation procedure was repeated three times, the membrane was suspended in binding buffer at a concentration of 900 ml buffer / original tissue (g) and used for the binding force assay. To a 96-well plate were added 180 μl of the membrane suspension, 10 μl each of the extract samples of Preparation Examples 1 to 6, Examples 1 to 7 and Comparative Examples 1 and 2 and Control Example 1, and ³ μl-flumazenil (1 nM, final concentration ) Were added and reacted in an ice-bath for 40 minutes. Then, they were collected using a glass fiber filter (GF / C, Whatman). The radioactivity of the samples was measured with Tri-Carb Liquid Scintillation Analyzers (Perkin-Elmer, Shelton, CT, USA). The nonspecific binding (NSB) was determined using clonazepam (1 μM, final concentration) and the binding displacement value (%) was calculated as follows.

Bond displacement (%) = [1 ((DPM NSBDPM of the sample) / (TB DPM NSB DPM)]] 100

Here, DPM is decomposition per minute, TB is total bond, and NSB is non-specific binding.

[ ³ H] -fluomazenyl bond displacement (%) in GABAA-BZD receptor Production Example 1 54 ± 2.1 Production Example 2 46 ± 3.2 Production Example 3 35 ± 1.9 Production Example 4 53 ± 0.8 Production Example 5 47 ± 1.1 Production Example 6 38 ± 0.5 Example 1 46 ± 0.8 Example 2 65 ± 1.3 Example 3 71 ± 2.4 Example 4 85 ± 0.2 Example 5 84 ± 0.8 Example 6 67 ± 0.6 Example 7 69 ± 0.9 Comparative Example 1 29 ± 0.8 Comparative Example 2 31 ± 0.8 Control Example 1 59 ± 0.8

The activity of inhibiting the binding reaction of ³ H-flumazenil to the receptor, which is a selective antagonist of the benzodiazepine receptor, was measured. As shown in the above Table 3, the crude extract of Preparation 1 (20% alcohol extract ) Was superior to the crude extract (60% alcohol extract) of Production Example 4, and the hop extract of Production Example 2 (20% alcohol extraction) was superior to the hop extract of Production Example 5 (60% 3 extract of lettuce (20% alcohol extract) was superior to the extract of lettuce (60% alcohol extract) of Preparation Example 6. In the following Examples, (20% alcohol extract) of Preparation Example 2 and a lettuce extract (20% alcohol extract) of Preparation Example 3 were used.

In addition, it was confirmed that the mixture of casein and potassium chloride of Example 1 was superior to casein alone of Comparative Example 1 and potassium chloride alone of Comparative Example 2. In Example 1, it was confirmed that the mixture of hop extract, lettuce extract or hop extract and lettuce extract (Example 4) containing 4 parts by weight of Rhizome vulgaris extract was found to be the most excellent.

Experimental Example  2: Example  4 and Comparative Example  Sleep-inducing effects of 1 to 3 Animal experiments

An ICR male mouse weighing 15 to 20 g was used as the sleep inducing test animal. Breeding conditions were maintained at 22 ± 2 ℃, 55 ± 5% relative humidity, 10-12 times per hour of ventilation, 12 hours of light cycle, and all experimental animals were adapted for one week in the laboratory animal room. Samples were administered once a day for 5 days. After 30 minutes of oral administration of the final sample, pentobarbital (30 mg / kg) was injected into mouse abdominal cavity to induce sleeping time (Table 4) and sleep latency Table 5) were measured. The control group was not administered.

sample Dose (mg / kg) Sleep time (minutes) Control group - 31 ± 3 Example 1 25 45 ± 6 90 51 ± 5 100 55 ± 2 Comparative Example 1 25 32 ± 3 90 35 ± 4 100 41 ± 5 Comparative Example 2 25 31 ± 3 90 34 ± 4 100 37 ± 5 Comparative Example 3 25 48 ± 1 90 51 ± 2 100 57 ± 3 Example 4 25 46 ± 4 90 59 ± 3 100 72 ± 2 Diazepam 2 77 ± 7

sample Dose (mg / kg) Elevation time (minutes) Control group - 8 ± 0.3 Example 1 25 7 ± 0.4 90 6 ± 0.3 100 6 ± 0.2 Comparative Example 1 25 8 ± 0.4 90 7 ± 0.3 100 7 ± 0.2 Comparative Example 2 25 8 ± 0.4 90 8 ± 0.2 100 7 ± 0.6 Comparative Example 3 25 7 ± 0.4 90 6 ± 0.3 100 5 ± 0.2 Example 4 25 6 ± 0.2 90 5 ± 0.3 100 4 ± 0.3 Diazepam 2 3 ± 0.3

As a result of the experiment, it was confirmed that the mixture of casein and potassium chloride of Example 1 alone was longer than that of casein alone of Comparative Example 1 and potassium chloride alone of Comparative Example 2, It was found that the included embodiment (Example 4) had the longest sleeping time and the shortest rise time.

Experimental Example  3: Example  4 Sleep-Induced Efficacy Simplified Clinical Trials

The questionnaire was administered to 30 adult men and women who were suffering from sleep disturbances. The subjects were divided into 15 groups according to the intake of the study group and the placebo group for 4 weeks. The contents of the questionnaire are shown in Table 6, and the score table is as shown in Table 7. The scores of each question are added together and the results are shown in Table 8.

Questionnaire question none slightly middle Severe Very severe 1. It's hard to fall asleep. 0 One 2 3 4 2. It is difficult to keep sleep. 0 One 2 3 4 3. It is difficult to fall asleep after waking up 0 One 2 3 4 4. Sleep satisfaction 0 One 2 3 4 5. Sleep quality and social life 0 One 2 3 4 6. Insomnia 0 One 2 3 4 7. Intervention of daytime activity 0 One 2 3 4

score result 0-7 There is no significant insomnia. 8-14 There is a slight insomnia tendency. 15-21 There is moderate insomnia. 22-28 There is severe insomnia.

Before ingestion Example 4
After ingestion Example 4
Improvement rate after ingestion (%) Placebo
Before ingestion Placebo
After ingestion Placebo
Improvement rate after ingestion (%) One 24 16 33.33 22 20 9.09 2 15 15 0 16 17 -6.25 3 11 6 45.45 10 8 20 4 24 12 90 23 22 4.34 5 12 5 58.33 11 10 9.09 6 13 10 23.08 15 14 6.67 7 15 One 93.33 13 12 7.69 8 22 2 90.91 21 18 14.29 9 16 9 33.33 17 15 11.76 10 22 10 43.75 20 20 0 11 13 7 54.55 15 15 0 12 23 6 46.15 21 20 4.76 13 18 3 83.33 17 15 11.76 14 16 One 93.75 20 19 5 15 17 4 76.47 18 16 11.11

As a result of the simple clinical results, it was confirmed that the sleeping disorder was improved by 90% or more on average in the group ingesting the Example 4, and that the Example 4 clearly improved the sleeping disorder and the sleep inducing effect when compared with the placebo control group there was.

While the invention has been shown and described with reference to certain exemplary embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (6)

10 to 90 parts by weight of casein or casein hydrolyzate and 2 to 10 parts by weight of potassium chloride. The method according to claim 1,
A composition for improving sleep disturbance and a composition for sleep inducing sleep characterized in that the composition for improving sleep disturbance and the composition for sleep inducing further comprise 1 to 10 parts by weight of a crude extract. 3. The method of claim 2,
The above-mentioned Porphyra grass extract is obtained by immersing a dried pterygium outpost in a 10 to 70% ethanol aqueous solution of 15 to 25 times the total amount of the building, extracting it at a temperature within a range of 60 to 90 ° C for 2 to 8 hours, Wherein the extract is concentrated and dried to be powdered. The method according to claim 1,
Wherein the sleep disorder improvement and sleep inducing composition further comprises 1 to 10 parts by weight of the hop extract or 1 to 30 parts by weight of the extract of lettuce. 5. The method of claim 4,
The hop extract is prepared by dipping the dried hopper in a 10 to 70% ethanol aqueous solution of 15 to 25 times the total amount of the building, extracting the mixture at a temperature within the range of 60 to 90 ° C for 2 to 8 hours, And the composition is pulverized, concentrated and dried to be powdered. 5. The method of claim 4,
The extract of lettuce is prepared by immersing the dried lettuce plant in a 10 to 70% aqueous solution of ethanol in an amount of 15 to 25 times the total amount of the building, extracting it at a temperature within the range of 60 to 90 ° C for 2 to 8 hours, Is concentrated and dried and powdered. ≪ RTI ID = 0.0 > 11. < / RTI >