KR101771848B1 - Device for analyzing a sample concentration using an interference of light and method for analyzing a sample concentration - Google Patents

Abstract

The present invention relates to a device to analyze a sample concentration using interference of light, and a method of analyzing sample concentration. According to the present invention, the device to analyze sample concentration using interference of light comprises: a guide indicating portion including at least two index areas which has a quantity of light of a first primary color light differently set by concentration of a material to be analyzed, and a nearby area in which the first primary color light is all absorbed or blocked, and a second and a third primary color lights reflected or emitted; and a bio-sensing channel portion disposed on the guide indicating portion having different absorption amount of the first primary color light emitted from the guide indicating portion by concentration of the material to be analyzed. By using an image visualized by a mixture of the first to third primary color lights emitted through the bio-sensing channel portion; the concentration of the material to be analyzed is determined.

Description

TECHNICAL FIELD [0001] The present invention relates to a sample concentration analyzing apparatus and a concentration analyzing method using a light interference phenomenon. [0002]

The present invention relates to a sample concentration analyzing apparatus and a concentration analyzing method using light interference phenomenon, and more particularly, to a sample concentration analyzing apparatus and a concentration analyzing method using color development phenomenon and light interference phenomenon of a biosensor.

Optical-based sensing systems are widely used in a variety of fields for the detection and analysis of materials because of their high sensitivity and selectivity. In particular, quantitative or qualitative analysis of analytes present in biological samples such as blood is very important in terms of chemistry and clinics, and thus optical-based sensing systems are widely used in the biosensor field.

In general, an optical-based sensing system includes a probe including an enzyme, a chromogen, a metal nanoparticle, a fluorescent substance or a quantum dot, which reacts with a specific substance, and a spectrometer such as a spectroscope for obtaining optical data using a light source As shown in FIG. Such an optically based sensing system is complex and bulky because it is composed of optical components such as lamps, filters, detectors, and prisms. In addition, since the spectroscope is an expensive device in which precise technology such as angle adjustment of the prism according to wavelength, position adjustment between the light source and the filter, and detector is concentrated, it is limited to use as a portable measurement device.

It is an object of the present invention to provide a sample concentration analyzing apparatus that can intuitively analyze the concentration of a sample while maintaining high sensitivity and selectivity, and is highly portable and utilizes a light interference phenomenon.

Another object of the present invention is to provide a concentration analysis method using the sample concentration analyzing apparatus.

The apparatus for analyzing the sample concentration using the interference phenomenon of light for one purpose of the present invention provides the first source color light, the second source color light and the third source color light, wherein the light quantity of the first source color light is different from each other A guide marking part including at least two different surface areas set differently from each other, a peripheral area which is absorbed or blocked by the first primary color light and provides the second primary color light and the third primary color light, And a biosensing channel unit in which the absorption amount of the first primary color light provided by the guide marking unit is changed according to the concentration of the substance to be analyzed, and the first to third primary color lights emitted through the biosensing channel unit are mixed with each other, To determine the concentration of the analyte.

In one embodiment, when the first circular color light provided by any one of the index regions is absorbed by the biosensing channel unit and the corresponding index region and the surrounding region are visually recognized in the same color, May be higher than the concentration of the analyte indicated in the corresponding region of the indicator.

In one embodiment, the indicator regions include first to n < th > indicator regions (where n is a natural number of 2 or more), and the x < th > indicator region (where x is a natural number of 1 or more and less than n) When the x + 1 index region is viewed in a color different from that of the peripheral region, the concentration of the analyte is equal to the concentration of the analyte indicated by the (x + 1) index region .

In one embodiment, the biosensing channel portion may include an oxidizing enzyme capable of oxidizing a target target substrate to generate hydrogen peroxide, and a peroxidase enzyme capable of converting a chromogenic dye substrate into a chromogenic product through a produced peroxidase horseradish peroxidase (HRP), and a mixture of chromogenic dye substrates that can be converted to chromogenic products by enzymatic reaction of mustard radish peroxidase in the presence of hydrogen peroxide. The substrate oxidase may be selected from the group consisting of glucose oxidase, galactose oxidase, lactate oxidase, pyruvate oxidase, glutamate oxidase, alcohol oxidase wherein the coloring dye substrate comprises at least one of alcohol oxidase, ascorbate oxidase, cholesterol oxidase and choline oxidase, wherein the coloring dye substrate is tetramethyl-benzidine , 3-diaminobenzidine (DAB) or 4-aminoantipyrine (4-AAP) and N-Ethyl-N- (2-hydroxy-3-sulfopropyl ) Or a mixture of 4-AAP and N-Ethyl-N- (2-hydroxy-3-sulfopropyl) -3-methoxyaniline (ADOS) (3-sulfopropyl) -3-methoxyaniline (ADPS), or a mixture of 4-AAP and N-Ethyl-N- or a mixture of 4-AAP and N-Ethyl-N- (2-hydroxy-3-sulfopropyl) -3,5-dimethoxyaniline (DAOS) A mixture of 4-AAP and N, N-bis (4-sulfobutyl) -3,5-dimethylaniline (MADB), or a mixture of 4-AAP And a mixture of 4-AAP and N-Ethyl-N- (2-hydroxy-3-sulfopropyl) -3-methylylaniline (TOOS) , Or a mixture of 4-AAP and N-Ethyl-N- (3-sulfopropyl) -3-methylaniline (TOPS).

In one embodiment, the biosensing channel may react with the analyte to absorb the first primary color light in the wavelength range of 520 nm to 600 nm or 600 nm to 670 nm.

In one embodiment, when the first circular color light is red light, the second circular color light and the third circular color light are green light and blue light, respectively, and when the first circular color light is blue light, When the first circular color light is green light, the second circular color light and the third circular color light may be red light and blue light, respectively.

In one embodiment, the amount of light of the second primary color light of the peripheral region and the surface regions is equal to each other, and the amount of light of the third primary color light of the peripheral region and the surface regions may be equal to each other.

In one embodiment, the apparatus for analyzing a sample concentration using the interference effect of light may further include an image display unit disposed on the biosensing channel unit and displaying an image represented by the biosensing channel unit on a screen.

In one embodiment, the image display unit may be a smart phone including a digital camera for photographing an image represented by the biosensing channel unit, and a display panel for displaying an image photographed by the digital camera. At this time, the biosensing channel unit may be coupled to the digital camera of the smartphone.

In one embodiment, the color change of the biosensing channel portion can be visually confirmed.

In one embodiment, the guide marking portion is in the form of paper in which the landmark regions and the peripheral region are printed in color, and can utilize the absorption and reflection of light by solar or illumination devices.

In one embodiment, the indicator regions and the peripheral region of the guide marking portion appear through a display panel of a smartphone incorporating illumination, and the biosensing channel portion may be disposed on the smartphone.

According to another aspect of the present invention, there is provided a concentration analyzing method comprising the steps of: providing a biosensor channel unit with an analyte having an unknown concentration that varies in absorption amount of a first primary color light according to a concentration of an analyte; At least two or more indicator regions which are arranged in the first circular color light, the second circular color light and the third circular color light and whose light amounts of the first primary color light are different from each other by the concentration of the substance to be analyzed, A mixture of first, second and third circularly polarized light beams emitted through the colored biosensing channel portion on a guide mark portion including a peripheral region providing both the second circular color light and the third circular color light, , Wherein the index regions are divided into a first index region to an nth index region, Wherein x is a natural number of 1 or more and less than n, is viewed in the same color as the peripheral region, and the (x + 1) -th indicator region is viewed in a different color from the peripheral region When it is confirmed, the concentration of the analyte is determined to be equal to the concentration of the analyte indicated as the (x + 1) th region.

According to the sample concentration analyzing apparatus and the concentration analyzing method using the light interference phenomenon of the present invention, the portability of the sample concentration analyzing apparatus having a simple structure can be improved. The sample concentration analyzer can intuitively visualize the concentration of the sample quantitatively while maintaining the sensitivity and selectivity at the level of the optical-based analyzer.

Particularly, the sample concentration analyzing apparatus is very easy to carry and can be applied to a widely popular smartphone, so that the sample concentration can be discriminated by visual observation of the displayed image or analysis of the photographed image without equipment for analysis There is an advantage to be able to do.

1 is a perspective view for explaining a sample concentration analyzing apparatus according to an embodiment of the present invention.
2 is a view for explaining reflection and absorption of the first to third circularly colored light beams in the guide markers and the biosensing channel portions of FIG.
FIG. 3 is a view for explaining reflection and absorption changes of first to third primary colors due to color development of the biosensing channel in the sample concentration analyzer of FIG. 1; FIG.
FIG. 4 is a perspective view of a sample concentration analyzing apparatus for explaining an image appearing due to color development of the biosensing channel unit in FIG. 3; FIG.
5 is a perspective view for explaining a sample concentration analyzing apparatus according to another embodiment of the present invention.
6 is a graph showing experimental data using a sample concentration analyzer for glucose.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. The present invention is capable of various modifications and various forms, and specific embodiments are illustrated in the drawings and described in detail in the text. It is to be understood, however, that the invention is not intended to be limited to the particular forms disclosed, but on the contrary, is intended to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the invention. Like reference numerals are used for like elements in describing each drawing.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In the present application, the term "comprises" or "having ", etc. is intended to specify that there is a feature, step, operation, element, part or combination thereof described in the specification, , &Quot; an ", " an ", " an "

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.

1 is a perspective view for explaining a sample concentration analyzing apparatus according to an embodiment of the present invention.

1, a sample concentration analyzing apparatus 501 includes a guide marking unit 100 and a biosensing channel unit 200. The apparatus includes a biosensing channel unit 200, IMG1) to analyze and determine the concentration of the analyte in the sample.

The guide marking portion 100 includes at least two or more surface regions DAa, DAb, DAc, DAd, and DAe and a peripheral region PA thereof. The guide marking unit 100 includes at least one or more units when defining a group of the land areas DAa, DAb, DAc, DAd, DAe and the surrounding area PA of the land areas as one unit can do. For example, three units as shown in FIG. 1 may constitute the guide marking unit 100. When two or more units constitute the guide marking unit 100, the chroma and brightness of the units may be different from each other. DAb, DAe, DAe) and the peripheral area (PA) in one unit and the relationship between the first area (DAa, DAb, DAc, DAd, DAe) The provision of the primary color light, the second primary color light and the third primary color light to the biosensing channel unit 200 will be described later with reference to Figs. 2 to 4. In the following, "provided with light" Means the amount of light reflected by the biosensing channel unit 200 due to the reflection of external light or the amount of light due to the self light emission.

Each of the surface areas DAa, DAb, DAc, DAd, and DAe is provided with a first circular color light, a second circular color light, and a third circular color light, wherein the light quantity of the first primary color light is set to be different . The three primary colors corresponding to the first through third primary colors may be red (R) green (G) and blue (B) light. The blue light has a wavelength of 430 nm to 470 nm, the green light has a wavelength of 510 nm to 550 nm, and the red light has a wavelength of 600 nm to 670 nm. For example, the first circular color light may be red light, and the second and third circular color light may be blue light and green light, respectively. Alternatively, the first circular color light may be green light, and the second and third circular color light may be red light and blue light, respectively.

The first indicator region DAa among the indicator regions DAa, DAb, DAc, DAd, and DAe corresponds to the indicator when the concentration of the analyte is low, and the second, third, fourth, (DAb, DAc, DAd, and DAe), the concentration of the analyte gradually increases. In this case, in the first indicator region DAa, which is an index indicating the case where the concentration of the analyte is low, among the indicator regions DAa, DAb, DAc, DAd, and DAe, The light amount of light can be set to be lower than the light amount of the first primary color light in the different index areas DAb, DAc, DAd, DAe exhibiting a higher concentration than the first index area DAa. The light amount of the first primary color light in the second land surface area DAb is higher than the light amount of the first primary color light in the first land surface area DAa and the light amount of the second primary color light in the third, fourth and fifth land areas DAc, DAd, DAe) of the first primary color light. That is, the light amount of the first primary color light in each of the land surface areas gradually increases from the first land area DAa to the fifth land area DAe.

In the peripheral area PA, all of the first circular color light is absorbed or blocked, and the second circular color light and the third circular color light are provided to the biosensing channel part 200. For example, in the peripheral region PA, all of the first circularly colored light is absorbed or blocked so that it can not be substantially reflected or emitted to the biosensing channel unit 200, and the second circularly colored light and the third circularly- And is reflected or emitted from the area PA and provided to the biosensing channel unit 200. [ At this time, the amounts of light of the second primary color light provided in the peripheral area PA and the surface areas DAa, DAb, DAc, DAd, and DAe may be substantially equal to each other. At the same time, the amount of light of the third primary color light provided in the peripheral area PA and the surface areas DAa, DAb, DAc, DAd, DAe may also be substantially equal to each other.

In other words, the second circular color light and the third circular color light in the peripheral area PA and the land areas DAa, DAb, DAc, DAd, and DAe have the same conditions, All of the color light is absorbed or blocked and the light amount of the first primary color light in the surface areas DAa, DAb, DAc, DAd, and DAe is reduced in each of the surface areas DAa, DAb, DAc, DAd, It is set differently depending on the concentration.

For example, the guide marking unit 100 may be in the form of paper in which the peripheral area PA and the surface areas DAa, DAb, DAc, DAd, and DAe are printed in color. At this time, the first to third primary color light can be absorbed and reflected by absorption and reflection of light by sunlight or external illumination device.

As another example, the periphery area PA and the land areas DAa, DAb, DAc, DAd, and DAe of the guide marking section 100 appear through a display panel of a smartphone incorporating illumination, The biosensing channel unit 200 may be disposed. At this time, the first to third primary colors may be emitted or interrupted by the emission or blocking of the display panel.

The biosensing channel unit 200 is disposed on the guide marking unit 100 and the absorption amount of the first primary color light provided by the guide marking unit 100 is changed according to the concentration of the analyte. The biosensing channel unit 200 has a characteristic of reacting with the analyte to develop color. Accordingly, the amount of absorption of the first primary color light varies depending on the color of the biosensing channel unit 200. However, the second and third primary colors of light provided by the guide marker unit 100 are substantially reflected by the biosensing channel unit 200 ). Accordingly, the mixture of the first through third circular color lights emitted through the biosensing channel unit 200 is viewed as an image IMG1 to the user.

When the biosensing channel unit 200 does not emit light, the first to third primary color lights of the respective surface regions DAa, DAb, DAc, DAd, and DAe are all transmitted to the biosensing channel unit 200 as shown in FIG. The image IMG1 of the biosensing channel unit 200 can be visually recognized for each of the surface regions DAa, DAb, DAc, DAd, and DAe.

The biosensing channel unit 200 has a characteristic of reacting with a substance to be analyzed to develop color, and the absorption amount of the first primary color light varies depending on the concentration of the substance to be analyzed. For example, when the concentration of the analyte is lower than the concentration of the analyte, the biosensing channel unit 200 absorbs more first color light. According to the characteristics of the biosensing channel unit 200, the concentration of the analyte can be analyzed and determined using the image IMG1 that can be visually recognized on the biosensing channel unit 200.

The biosensing channel unit 200 oxidizes the target substrate to generate oxidized enzyme capable of generating hydrogen peroxide, and a horseradish peroxidase enzyme capable of changing the chromogenic substrate of the chromogenic dye substrate through the generated peroxidase , HRP) and a mixture of chromogenic dye substrates which can be converted to chromogenic products by enzymatic reaction of mustard non-peroxidase in the presence of hydrogen peroxide.

At this time, the substrate oxidase includes glucose oxidase, galactose oxidase, lactate oxidase, pyruvate oxidase, glutamate oxidase, alcohol oxidase alcohol oxidase, ascorbate oxidase, cholesterol oxidase, choline oxidase and the like can be used. Tetramethyl-benzidine (TMB) or 3,3-diaminobenzidine (DAB) may be used as the coloring dye substrate, or 4-aminoantipyrine (4-AAP ) And N-Ethyl-N- (2-hydroxy-3-sulfopropyl) -3,5-dimethylaniline (MAOS) 3-methoxyaniline (ADOS), or a mixture of 4-AAP and N-Ethyl-N- (3-sulfopropyl) -3-methoxyaniline ) or a mixture of 4-AAP and N-Ethyl-N- (2-hydroxy-3-sulfopropyl) -3,5-dimethoxyaniline (DAOS) AAP and a mixture of 4-sulfobutyl) -3,5-dimethylaniline (MADB), or a mixture of 4-AAP and N, N-bis A mixture of 4-AAP and N-Ethyl-N- (2-hydroxy-3-sulfopropyl) -3-methylaniline (TOOS) 4-AAP and N-Ethyl-N- (3 -sulfopropyl) -3-methylaniline (TOPS) and the like can be used.

For example, the biosensing channel section 200 may comprise a mixture of glucose oxidase, mustard radish peroxidase (HRP), 4-aminoantipyrine (4-AAP) and MAOS, It is possible to change to a blue-green color system having a property of absorbing red light. As another example, the biosensing channel section 200 may include tetramethyl-benzidine (TMB), which reacts with mustard non-peroxidase to form a complex. TMB reacts with HRP to form a complex to absorb red light.

Hereinafter, with reference to FIG. 1, FIG. 2, FIG. 3, and FIG. 4, description will be made on the case where the biosensing channel unit 200 is developed and the concentration of the analyte.

FIG. 2 is a view for explaining the reflection and absorption of the first to third circularly colored light beams in the guide marking unit and the biosensing channel unit of FIG. 1, and FIG. And reflection and absorption changes of the first to third primary colors due to color development.

The graphs of (a), (b), (c), (d) and (e) in each of FIGS. 2 and 3 are graphs of the first, second and third graphs in each of the surface areas DAa, DAb, DAc, DAd, The first circular color light is defined as a red light R, and the second and third circular color light are defined as a blue light B and a green light G, respectively. The graph indicated by "(PA)" in FIG. 2 represents the amounts of light of the first to third circularly polarized light components in the peripheral area PA. The "light amount" at this time means the intensity of light provided to the biosensing channel unit 200 in the guide marking unit 100.

Referring first to FIG. 2 together with FIG. 1, the light amounts of the second primary color lights of the surface areas DAa, DAb, DAc, DAd, and DAe are equal to each other and the light amounts of the third primary color light are equal to each other. The light amount of the second primary color light of the peripheral area PA is the same as that of the surface areas DAa, DAb, DAc, DAd, DAe, DAb, DAc, DAd, DAe).

The light intensity of the first primary color light in the surface areas DAa, DAb, DAc, DAd, and DAe and the peripheral area PA in a state in which the biosensing channel unit 200 does not react with the analyte, Since the biosensing channel unit 200 transmits all of the first to third primary color lights, the user can use the first to third primary color lights passing through the biosensing channel unit 200 to mix the surface areas DAa and DAb , DAc, DAd, and DAe) and the peripheral area (PA), respectively. That is, on the biosensing channel unit 200, the guide markers 100 are recognized as they are, and the user can view the image IMG1 of FIG.

Referring to FIG. 3 together with FIGS. 1 and 2, when the biosensing channel unit 200 reacts with the analyte, the biosensing channel unit 200 absorbs the first color light, The color light is transmitted. Thus, at least one of the land areas DAa, DAb, DAc, DAd, and DAe is not visually recognized. However, the amount of absorption of the first primary color light of the biosensing channel unit 200 varies depending on the concentration of the analyte.

For example, when the concentration of the substance to be analyzed is higher than the index indicated by the third indicator region DAc, the first to third indicator regions DAa, DAb, DAc are provided to the biosensing channel unit 200 1 primary color light is absorbed by all of the biosensing channel unit 200 and the first original color light provided by the fourth and fifth index regions DAd and DAe to the biosensing channel unit 200 is transmitted or absorbed only partially And is discharged to the upper portion of the biosensing channel unit 200. Accordingly, when the user views the biosensing channel unit 200, the first to third land areas DAa, DAb, DAc and the peripheral area PA reflecting only the second and third primary color lights are substantially The user can not recognize the first to third indicator areas DAa, DAb, DAc. However, since the first circular color light provided to the biosensing channel unit 200 by the fourth and fifth land regions DAd and DAe passes or is absorbed only partially and is emitted to the upper portion of the biosensing channel unit 200 The fourth and fifth landmarks DAd and DAe can be distinguished from the surrounding area PA on the biosensing channel unit 200. [

FIG. 4 is a perspective view of a sample concentration analyzing apparatus for explaining an image appearing due to color development of the biosensing channel unit in FIG. 3; FIG.

Referring to FIG. 4 together with FIG. 3, when the biosensing channel unit 200 reacts with the analyte, the user recognizes the image IMG2 shown in FIG. 4 on the biosensing channel unit 200. The first to third indicator regions DAa to DAc appear substantially identical to the peripheral region PA that provides only the second and third primary color lights and the fourth and fifth indicator regions DAd, DAe) can be recognized separately from the peripheral wall area PA.

According to this principle, when the biosensing channel unit 200 reacts with the analyte, the user can not visually recognize the first to third indicator regions DAa, DAb, and DAc, The concentration of the substance to be analyzed can be analyzed and determined as being substantially higher than the concentration represented by the third indicator region DAc. Based on the third indicator region DAc, the concentration of the analyte can be inferred to be higher than the concentration of the analyte indicated in the corresponding indicator region, i.e., the third indicator region DAc. At the same time, Since the region DAd is visually observed, it can be analyzed and determined as the concentration of the analysis target actually indicated by the fourth land surface region DAc.

1 to 4 illustrate the case where five unitary areas DAa, DAb, DAc, DAd, and DAe are included in one unit. However, the first to n-th ground areas , and n is a natural number of 2 or more). In this case, the x-th surface region (where x is a natural number of 2 or more and less than n) among the n number of surface regions is visually observed in the same color as the surrounding region PA, and the x + In the case of being viewed in different colors, the concentration of the analyte can be analyzed and determined to be the same as the concentration of the analyte indicated in the (x + 1) th region.

As described above, the sample concentration analyzing apparatus 501 can intuitively visually analyze the concentration of the sample quantitatively while maintaining high sensitivity and selectivity at the level of an expensive optical-based analyzer.

5 is a perspective view for explaining a sample concentration analyzing apparatus according to another embodiment of the present invention.

5, the sample concentration analyzing apparatus 502 includes a guide marking unit 100, a biosensing channel unit 200, and an image display unit 300. The sample concentration analyzing apparatus 502 of FIG. 5 is substantially the same as the sample concentration analyzing apparatus 501 described in FIGS. 1 to 4 except that it further includes the image displaying unit 300. Therefore, redundant detailed description will be omitted.

The image display unit 300 is disposed on the biochemical sensing channel unit 200 in a state where the guide marking unit 100 and the biosensing channel unit 200 are sequentially stacked. The image display unit 300 displays an image (IMG1 in FIG. 1 or IMG2 in FIG. 4) represented by the biosensing channel unit 200 on the screen.

The image display unit 300 may be a smart phone including a digital camera that captures an image represented by the biosensing channel unit 200 and a display panel that displays an image captured by the digital camera. The biosensing channel unit 200 can be coupled to the digital camera of the image display unit 300.

As shown in FIG. 5, the sample concentration analyzing apparatus 502 is very easy to carry and can be applied to a smartphone widely publicized, so that it is possible to perform visual observation of an image displayed without equipment for analysis, There is an advantage that the sample concentration can be determined by the analysis alone. Accordingly, the portability and versatility of the sample concentration analyzer 502 can be improved.

Hereinafter, a sample concentration analyzing apparatus for glucose capable of analyzing the concentration of glucose is constructed substantially the same as the sample concentration analyzing apparatus 502 shown in FIG. 5, and the results of the experiment are described with reference to FIG.

The concentration of glucose indicated by each of the first to fifth indicator regions DAa, DAb, DAc, DAd and DAe in FIG. 5 was sequentially 0 mM, 5 mM, 6 mM, 7 mM, 8 mM and 9 mM , The biosensing channel unit 200 used glucose oxidase (GOx), HRP, 4-AAP and MAOS. In this sample concentration analyzer for glucose, changes in absorbance of each of the wavelengths of the biosensing channel unit 200 were measured at a glucose concentration of 0 mM, and the concentration of glucose was 5 mM, 6 mM, 7 mM, 8 mM mM and 9 mM, respectively. Also, the change in transmittance was measured at each wavelength. The results are shown in Fig.

6 is a graph showing experimental data using a sample concentration analyzer for glucose.

6 is a photograph showing the color change of the biosensing channel unit 200 according to the glucose concentration, FIG. 2 is a photograph of the image observed on the biosensing channel unit 200 according to the glucose concentration, And FIG. 4 is a graph showing a change in transmittance according to wavelengths. FIG. 4 is a graph showing the change in absorbance of each wavelength of the biosensing channel unit 200. FIG. 6 is a graph showing the results of measuring the intensity of light emitted from the surface regions and the peripheral region with time, and Fig. 6 is a graph showing the intensity of light emitted from the surface regions and the peripheral region, And the results are shown in FIG.

Referring to FIG. 6, the biosensing channel unit 200 emits blue light and green light and absorbs red light, as shown in FIG. 6 (a). As the concentration of glucose increases, Able to know. 6, when the glucose concentration is 0 mM, the first to fifth indicator regions DAa, DAb, DAc, DAd, and DAD are displayed in the image according to the change of the biosensing channel unit 200, Only the second to fourth indicator regions DAb, DAc, DAd, and DAe are visible when the color of the first indicator region DAa is equal to the surrounding region PA. It can be seen that only the fourth and fifth land areas DAd and DAe are visible as the colors of the first to third land areas DAa, DAb and DAc become equal to the surrounding area PA. It can be confirmed that the first to fifth index regions DAa, DAb, DAc, DAd, and DAe are all the same color as the peripheral region PA and are not distinguished when the concentration of glucose is 9 mM.

Referring to FIG. 6 (3), it can be seen that the absorption amount of red light having a wavelength of 600 nm to 650 nm actually increases as the concentration of glucose increases. That is, the degree of absorbing the red light reflected by the first to fifth index regions DAa, DAb, DAc, DAd, and DAe of the guide marking portion varies depending on the absorption amount of the red light of the biosensing channel portion, And can be viewed with the same image.

6, in contrast to ③ in FIG. 6, red light having a wavelength of 600 nm to 650 nm in transmittance has a lower transmittance as the concentration of glucose increases and light having a wavelength other than red light .

In addition, referring to (5) in FIG. 6, it can be seen that the light emitted from the peripheral region and the emitted light substantially coincide gradually with time (from 1 minute to 5 minutes). 6, it can be seen that as the concentration of glucose increases (from 0 mM to 9 mM), the light components in the surface region and the peripheral region substantially coincide with each other. This is a result of proving that the surface region is assimilated to the surrounding region due to the optical interference phenomenon and the shape of the surface region disappears without being recognized.

Based on the results of the experiment and the resultant data of FIG. 6, the concentration of the analyte, such as glucose, is actually analyzed using the sample concentration analyzing apparatuses 501 and 502 according to the present invention described in FIGS. 1 to 5 And discrimination can be easily performed.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention as defined by the following claims. It can be understood that it is possible.

501, 502: sample concentration analyzer
100: Guide mark part
200: Biosensing channel section
300: image display unit
DAa, DAb, DAc, DAd, DAe: first, second, third, fourth, fifth land regions
PA: peripheral area

Claims (15)

Wherein each of the at least two surface regions includes at least two surface regions and a peripheral region of the at least one surface region, wherein each of the at least one surface region provides a first primary color light, a second primary color light and a third primary color light, Wherein the guide markers provide only the second primary color light and the third primary color light, and the light quantity of the first primary color light provided by the surface areas is different for each of the surface areas; And
The first marker light is absorbed by the first marker light, and the first primary color light is absorbed by the guide marker. And a biosensing channel portion having a different absorption amount of light,
A first circular color light provided by the biosensing channel part after the first circular color light provided to the biosensing channel part is absorbed by the biosensing channel part in the guide mark part and the first circular color light provided by the guide cover part, Wherein the concentration of the analyte injected into the biosensor channel portion is discriminated through the color of the biosensing channel portion by mixing the second and third primary color lights passing through the biosensing channel portion.
A sample concentration analyzer using light interference effect.
The method according to claim 1,
The first primary color light provided by any one of the surface regions of the guide markers is absorbed by the biosensing channel unit and is detected by the biosensing channel unit corresponding to the corresponding surface region and the surrounding region, If the color of the area appears the same,
Wherein the concentration of the analyte injected into the biosensor channel portion is higher than the concentration of the analyte indicated by the indicator region.
A sample concentration analyzer using light interference effect.
The method according to claim 1,
Wherein the indicator regions include a first indicator region to an nth indicator region, where n is a natural number greater than or equal to 2, and wherein the x < th > indicator region (where x is a natural number greater than or equal to 1 and less than n) And a color appearing in the biosensing channel part corresponding to the (x + 1) -th indicator area corresponds to a color appearing in the biosensing channel part corresponding to the peripheral area, If it differs from the color that appears in,
And the concentration of the analyte is equal to the concentration of the analyte indicated as the (x + 1) -th indicator region.
A sample concentration analyzer using light interference effect.
The method according to claim 1,
The biosensing channel unit
The presence of hydrogen peroxide (HRP) and horseradish peroxidase (HRP), which can convert the chromogenic dye substrate into a chromogenic product through oxidative enzymes capable of generating hydrogen peroxide by oxidizing the target substrate and generated peroxidase, Characterized in that it comprises a mixture of chromogenic dye substrates which can be converted into chromogenic products by an enzymatic reaction of mustard radish peroxidase under conditions of < RTI ID = 0.0 >
A sample concentration analyzer using light interference effect.
5. The method of claim 4,
The substrate oxidase
It is known that glucose oxidase, galactose oxidase, lactate oxidase, pyruvate oxidase, glutamate oxidase, alcohol oxidase, ascorbic acid Wherein the enzyme comprises at least one of an oxidase, an oxidase, a cholesterol oxidase, and a choline oxidase,
The coloring dye substrate is
Tetramethyl-benzidine (TMB) or 3,3-Diaminobenzidine (DAB) may be used independently, or 4-aminoantipyrine (4-AAP) and N-Ethyl- (2-hydroxy-3-sulfopropyl) -3,5-dimethylaniline (MAOS) or a mixture of 4-AAP and N-Ethyl-N- , Or a mixture of 4-AAP and N-Ethyl-N- (3-sulfopropyl) -3-methoxyaniline (ADPS) or a mixture of 4-AAP and N-Ethyl-N- (3-sulfopropyl) aniline Or a mixture of 4-AAP and N-Ethyl-N- (2-hydroxy-3-sulfopropyl) -3,5-dimethoxyaniline (DAOS) , A mixture of 4-AAP and N, N-bis (4-sulfobutyl) -3,5-dimethylaniline (MADB), or a mixture of 4-AAP and 5-dimethoxyaniline AAP and N-Ethyl-N- (2-hydroxy-3-sulfopropyl) -3-methylaniline (TOOS), or a mixture of 4-AAP and N- -N- (3-sulfopropyl) -3-methyllaniline (TO PS) is used.
A sample concentration analyzer using light interference effect.
5. The method of claim 4,
The biosensing channel unit
Characterized in that it reacts with the substance to be analyzed to absorb the first primary color light in the wavelength range of 520 nm to 600 nm or 600 nm to 670 nm.
A sample concentration analyzer using light interference effect.
The method according to claim 1,
The first circular color light is red light, the second circular color light and the third circular color light are green light and blue light, respectively,
When the first circular color light is blue light, the second circular color light and the third circular color light are red light and green light, respectively,
Wherein when the first circular color light is green light, the second circular color light and the third circular color light are red light and blue light, respectively,
A sample concentration analyzer using light interference effect.
The method according to claim 1,
The amount of light of the second primary color light of the peripheral region and the surface regions is equal to each other,
And the amount of light of the third primary color light of the peripheral region and the surface regions is equal to each other.
A sample concentration analyzer using light interference effect.
The method according to claim 1,
And an image display unit disposed on the biosensing channel unit for displaying an image represented by the biosensing channel unit on a screen.
A sample concentration analyzer using light interference effect.
10. The method of claim 9,
The image display unit
A digital camera for photographing an image represented by the biosensing channel unit; and a display panel for displaying an image taken by the digital camera.
A sample concentration analyzer using light interference effect.
11. The method of claim 10,
Wherein the biosensing channel unit is coupled to the digital camera of the smartphone.
A sample concentration analyzer using light interference effect.
The method according to claim 1,
Characterized in that the color represented by the biosensor channel is visible to an observer.
A sample concentration analyzer using light interference effect.
The method according to claim 1,
Wherein the guide marking portion is in the form of paper on which the index regions and the peripheral region are printed in color,
Characterized in that absorption and reflection of light by sunlight or lighting devices is utilized,
A sample concentration analyzer using light interference effect.
The method according to claim 1,
Wherein the indicator regions and the surrounding region of the guide marking portion are displayed through a display panel of a smartphone incorporating illumination, and the biosensing channel portion is disposed on the smartphone.
A sample concentration analyzer using light interference effect.
Providing a biosensing channel unit with an analyte having an unknown concentration in which an absorption amount of the first primary color light varies according to a concentration of the analyte; And
Wherein the at least one of the at least two surface regions and the at least one of the at least one surface regions is provided with a first circular color light, a second circular color light, and a third circular color light, Wherein the peripheral region provides only the second primary color light and the third primary color light without providing the first primary color light and the light quantity of the first primary color light provided by the index regions is different for each of the index regions, The first circular color light provided by the biosensing channel unit after the first circular color light provided to the biosensing channel unit is absorbed by the biosensing channel unit and the first circular color light provided by the guide cover unit and passing through the biosensing channel unit, The concentration of the analyte injected into the biosensor channel portion through the color represented by the biosensor channel portion by the mixing of the first and second primary color light components Includes; step of each
At least two or more of the first and second circularly polarized light beams are provided such that the first circularly polarized light, the second circularly polarized light, and the third circularly polarized light are arranged such that the light amount of the first primary color light is different from that of the analyte, Second and third circularly polarized light emitted through the colored biosensing channel portion on the guide marking portion including the peripheral region providing the second circularly polarized light and the third circularly polarized light, And determining the concentration of the analyte through the analyzer,
Wherein the color of the biosensing channel corresponding to the first to n < th > surface regions (where n is a natural number of 2 or more) of the surface regions is equal to the color of the biosensing channel portion corresponding to the peripheral region, When the color of the biosensing channel corresponding to the +1 index region is different from the color of the biosensing channel corresponding to the peripheral region, the concentration of the analyte is the concentration of the analyte indicated by the (x + 1) Is determined to be the same as "
Method of analyzing sample concentration using interference effect of light.

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