KR101730033B1 - Paper pH-sensor using colorimetry and method of manufacturing the same - Google Patents

Abstract

The present invention relates to a paper sheet comprising a fluid passage made of a hollow-paper passage, in which a detection section is formed in which a plurality of detection areas are arranged along a longitudinal direction of the fluid passage; An upper hydrophilic film disposed on the upper surface of the paper layer and having a fluid inlet and / or an outlet; And a lower hydrophilic film disposed on a lower surface of the paper layer; Wherein the paper layer is made of a hydrophobic member except for the inner surface of the fluid passage and the detection portion, and each of the plurality of detection regions is impregnated with each indicator for inducing a color change at pH 0 to pH 14 To a paper pH sensor using a colorimetric method.

Description

TECHNICAL FIELD The present invention relates to a paper pH sensor using colorimetry and a method of manufacturing the same,

The present invention relates to a paper pH sensor using colorimetry and a method for producing the same.

pH (hydrogen ion concentration) is a numerical value indicating the degree of acidity or alkalinity of a substance and is used in various fields such as chemistry, biology, and medicine.

Conventionally, to measure pH, litmus spp. Or indicator was used. The pH value was measured by changing color of litmus spp. Or indicator according to pH. However, such a method using litmus species or indicator has a limitation in accuracy. For example, a roll type paper pH sensor (Advantec, pH Test Paper, 07011030) distinguishes colors by the naked eye and measures the pH value. In order to solve this problem, a strip type pH sensor (SIGMA, pH Test Strips, P-4786) is used in a plurality of regions in an accurate expression color according to a pH value. However, since the above-mentioned strip type pH sensor has to determine the pH value by comparing the color change of several detector parts with the standard color change chart provided by the manufacturer, it is necessary for the user to understand the usage of the product and to learn the usage method , It is difficult to discriminate the color at pH 0 ~ 3, pH 7 ~ 9 and pH 11 ~ 14 in case of an unskilled ordinary person, so it is difficult to measure the pH accurately.

However, since the method using the litmus paper and the indicator has a limitation in accuracy, recently, in order to measure more accurately, the pH in the solution is measured using a direct potential difference method between a measuring electrode using a glass electrode and a reference electrode, Analysis methods are widely used. However, the measurement sensor using such a glass electrode is stable and has a long lifetime and is widely used, but has a disadvantage in that the response speed is slow.

KR 10-0631276 (registration number)

The present invention provides a paper pH sensor using a colorimetric method capable of quickly and accurately measuring a pH value and a method for producing the same.

The present invention relates to a paper sheet comprising a fluid passage made of a hollow-paper passage, in which a detection section is formed in which a plurality of detection areas are arranged along a longitudinal direction of the fluid passage; An upper hydrophilic film disposed on the upper surface of the paper layer and having a fluid inlet and / or an outlet; And a lower hydrophilic film disposed on a lower surface of the paper layer; Characterized in that the paper layer is made of a hydrophobic member except for the inner surface of the fluid passage and the detection portion and each of the plurality of detection regions is impregnated with each reagent for inducing a color change at pH 0 to pH 14 The pH of the paper is measured using a colorimetric method.

The present invention relates to a paper sheet comprising a fluid passage made of a hollow-paper passage, in which a detection section in which a plurality of detection areas are arranged along a longitudinal direction of the fluid passage is formed; An upper adhesive layer disposed on the upper surface of the paper layer and having a fluid inlet and / or an outlet; A lower hydrophilic film disposed on a lower surface of the paper layer; And a lower adhesive layer disposed on a lower surface of the lower hydrophilic film; Characterized in that the paper layer is made of a hydrophobic member except for the inner surface of the fluid passage and the detection portion and each of the plurality of detection regions is impregnated with each reagent for inducing a color change at pH 0 to pH 14 The pH of the paper is measured using a colorimetric method.

(A) producing a paper layer comprising a hollow-paper path; (b) preparing an upper hydrophilic film and a lower hydrophilic film; And (c) disposing the upper hydrophilic film and the lower hydrophilic film on the upper and lower surfaces of the paper layer, and fixing the upper hydrophilic film and the lower hydrophilic film using a pressing process of adhesion or adhesion; The present invention also provides a method of manufacturing a paper pH sensor using a colorimetric method.

(A) producing a paper layer comprising a hollow-paper path; (b) fabricating an upper adhesive layer and a lower adhesive layer; And (c) disposing the upper adhesive layer and the lower adhesive layer on the upper and lower surfaces of the paper layer, and fixing the upper and lower adhesive layers using a pressing process of adhering. In the step (c) Inserting a paper layer and a lower hydrophilic film into the lower hydrophilic film and pressing and fixing the lower adhesive layer using a pressing process; The present invention also provides a method of manufacturing a paper pH sensor using a colorimetric method.

The pH value of the sample to be detected can be measured quickly and accurately using the color pH sensor using the colorimetric method according to the present invention.

1 is a cross-sectional view of a paper pH sensor using the colorimetric method of the present invention.
2 is a cross-sectional view of a paper pH sensor using the colorimetry method of the present invention.
3 is an exploded perspective view of a paper pH sensor using the colorimetric method of the present invention.
4 is a sectional view of the paper pH sensor using the colorimetric method of the present invention.
5 is an exploded perspective view of a paper pH sensor using the colorimetric method of the present invention.
6 is a view illustrating a process of manufacturing a paper pH sensor using the colorimetric method according to the first embodiment of the present invention.
FIG. 7 is a graph showing changes in contact angle with water according to elapsed time after plasma treatment of a PET surface in Example 1 of the present invention. FIG.
8 is a view showing a paper pH sensor using the colorimetric method produced in Example 2 of the present invention.
9 is an exploded perspective view of the paper pH sensor using the colorimetric method produced in Example 3 of the present invention.
10 is an exploded perspective view of the paper pH sensor using the colorimetric method manufactured in Example 4 of the present invention.
11 is an exploded perspective view of the paper pH sensor using the colorimetric method manufactured in Example 5 of the present invention.
12 is a graph showing the fluid movement speed for the fluid flow devices fabricated from the plasma-treated PET film in Experimental Example 1 of the present invention.
13 is a graph showing the fluid movement speed for the fluid flow devices fabricated from the PET film without plasma treatment in Experimental Example 1 of the present invention.
FIG. 14 is a graph showing the results of measurement of the pH of a sample using the color pH sensor using the colorimetric method prepared in Example 1 of the present invention ((A) pH 5, (B) pH 7, 8).
15 is a graph showing the result of measuring the pH of a sample using the paper pH sensor using the colorimetric method prepared in Example 1 of the present invention.
16 is a graph showing the result of measuring the pH of a sample using a pH sensor including a paper passage as a comparative example.
17 is a graph showing the results of measurement of the pH of a sample using a paper pH sensor using the colorimetric method prepared in Example 2 of the present invention.
18 is a diagram showing the result of measuring the pH of a sample using the paper pH sensor using the colorimetric method prepared in Example 3 of the present invention.
19 is a graph showing the result of measurement of the pH of a sample using the paper pH sensor using the colorimetric method prepared in Example 4 of the present invention.
20 is a diagram showing the result of measuring the pH of a sample using the paper pH sensor using the colorimetric method manufactured in Example 5 of the present invention.
21 is a graph showing the result of measuring the pH of a sample using the paper pH sensor using the colorimetric method prepared in Example 1 of the present invention.
22 is a graph showing the result of measuring the pH of a sample using the paper pH sensor using the colorimetric method prepared in Example 1 of the present invention.
23 is a graph showing the results of measurement of the pH of a sample using a paper pH sensor using the colorimetric method prepared in Example 1 of the present invention.

The present invention relates to a paper pH sensor using a colorimetric method capable of quickly and accurately measuring a pH value.

More specifically, the paper pH sensor using the colorimetric method of the present invention includes a paper layer in which a detection section is formed, which includes a fluid passage made of a hollow-paper passage, and in which a plurality of detection areas are arranged along the longitudinal direction of the fluid passage, And a lower hydrophilic film disposed on a lower surface of the paper layer, wherein the paper layer is a hydrophobic member except the inner surface of the fluid passage and the detection portion, And the plurality of detection regions are impregnated with respective reagents which induce a color change at pH 0 to pH 14.

In one embodiment, the upper hydrophilic film and / or lower hydrophilic film can be attached to the upper and / or lower surface of the paper layer by an adhesive layer.

Meanwhile, the upper hydrophilic film may be in close contact with the intermediate adhesive layer, and the intermediate adhesive layer may have a space to accommodate the paper layer and the lower hydrophilic film. In this case, And further comprising:

At least one of the upper hydrophilic film and the lower hydrophilic film may be a transparent substrate, and the paper layer or the upper adhesive layer may include marking means in a region corresponding to the detection region.

In a specific embodiment, the paper pH sensor using the colorimetric method of the present invention includes a paper layer on which a detection section is formed, which includes a fluid passage made of a hollow-paper passage, and a plurality of detection areas are arranged along the longitudinal direction of the fluid passage; An upper adhesive layer disposed on the upper surface of the paper layer and having a fluid inlet and / or an outlet; A lower hydrophilic film disposed on a lower surface of the paper layer; And a lower adhesive layer disposed on a lower surface of the lower hydrophilic film; Characterized in that the paper layer is made of a hydrophobic member except for the inner surface of the fluid passage and the detection portion and each of the plurality of detection regions is impregnated with each reagent for inducing a color change at pH 0 to pH 14 .

At this time, the paper layer and the lower hydrophilic film are in close contact with each other by the upper adhesive layer and the lower adhesive layer, and an intermediate layer (space) is formed between the upper adhesive layer and the lower adhesive layer to accommodate the paper layer and the lower hydrophilic film 190) to uniformly adhere the paper layer, the upper adhesive layer and the lower hydrophilic film to the lower adhesive layer.

Particularly, the upper adhesive layer and the lower adhesive layer are characterized in that an adhesive material is applied on one surface.

As a specific aspect, the paper layer or the upper adhesive layer of the present invention may be provided with marking means in a region corresponding to the detection region.

In addition, the reagent is made up of a pH indicator, which can be a malachite, brilliant green, methyl green, methyl violet, crystal violet, eosin B bluish, ethyl violet, m-cresol purple, thymol blue, p-xylenol blue, 2,2 ', 2', 4 , 4'-pentamethoxy-triphenylcarbinol, quinaldine red, 2,4-dinitrophenol (2,4- dinitrophenol, methyl yellow, bromochlorophenol, bromophenol blue, tetrabromophenol blue, congo red, methyl orange, Bromocresol green, 2,5-dinitrophenol, methyl red, chlorophenol red, bromo, Bromocresol purple, bromophenol red, nitrazine yellow, bromoxylenol blue, bromothymol blue, neutral red, phenol red, phenol red, 3-nitrophenol, 1-naphtholphthalein, phenolphthalein, thymolphthalein, alizarin yellow GG, tripolein O at least one selected from the group consisting of tropaeolin O, indigo carmine, epsilon blue, alkali blue, and titan yellow.

On the other hand, the reagent may comprise at least one pH indicator and an auxiliary compound which stabilizes the indicator in an aqueous solution. More specifically, the reagent comprises an indicator aqueous solution comprising at least one of the pH indicator and an auxiliary compound having a high interaction with the pH indicator, wherein the indicator aqueous solution has an acid dissociation constant (pKa) of the pH indicator Can be changed.

More specifically, the auxiliary compound may be composed of an organic compound which causes an ion-ion or ion-dipole interaction with a pH indicator, and the auxiliary compound may be cetyltrimethyl ammonium sulfate, dodecyl pyridinium bromide pyridinium bromide, sodium dodecyl sulfate, sodium dodecyl sulfonate, sodium hexadecanoate, 4- (1,1,3,3-tetramethylbutyl) Phenyl- polyethylene glycol, 4- (1,1,3,3-tetramethylbutyl) phenyl-polyethylene glycol, dodecyl penta (ethylene oxide), trihexyl- (tetradecyl) phosphonium chloride triethyl- (tetradecyl) phosphonium chloride, trimethylpyrazolium methylsulfate, 1-butyl-3-methylimidazoli um hydrogen sulfate, and 1-ethyl-3-methylimidazolium chloride.

In addition, the present invention relates to a method of producing a paper pH sensor using a colorimetric method, comprising the steps of: (a) producing a paper layer comprising a hollow-paper path; (b) preparing an upper hydrophilic film and a lower hydrophilic film; And (c) disposing the upper hydrophilic film and the lower hydrophilic film on the upper and lower surfaces of the paper layer, and fixing the upper hydrophilic film and the lower hydrophilic film using a pressing process of adhesion or adhesion; . ≪ / RTI >

In this case, the step (a) may include forming the outer wall of the fluid passage and the detection area hydrophobic in the paper layer; Forming a hollow-paper passage by using a CO ₂ cutter at a portion spaced apart from the outer wall by a predetermined distance to the inside of the fluid passage; And impregnating the detection area with a reagent.

On the other hand, the step of hydrophobicly forming the outer wall of the fluid passage and the detection area in the paper layer described above may be performed by photolithography, ink-jet, wax printing, impregnation & hardening ), Imprinting, and screen printing. ≪ IMAGE >

In addition, the step (b) may include a step of cutting the upper hydrophilic film to form a fluid inlet and a detection area, wherein the step (c) includes the step of bonding the paper layer to the upper hydrophilic film Adhering between the lower hydrophilic film; And pressing the upper hydrophilic film, the lower hydrophilic film and the paper layer attached by the adhesive layer.

At this time, the step of pressing the upper hydrophilic film, the lower hydrophilic film and the paper layer may be performed by thermocompression at a temperature of 45 to 95 ° C.

Meanwhile, as a specific aspect, the step (c) may include inserting a paper layer and a lower hydrophilic film into an intermediate adhesive layer having a space therein, and adhering and fixing the lower adhesive layer under the lower hydrophilic film, The lower adhesive layer may be thermocompression-bonded under the condition of 70 to 130 DEG C by using a lamination film and placing the lower adhesive layer under the lower hydrophilic film.

In another aspect, a method for producing a paper pH sensor using the colorimetric method of the present invention comprises the steps of: (a) preparing a paper layer comprising a hollow-paper path; (b) fabricating an upper adhesive layer and a lower adhesive layer; And (c) disposing the upper adhesive layer and the lower adhesive layer on the upper and lower surfaces of the paper layer, and fixing the upper and lower adhesive layers using a pressing process of adhering. In the step (c) Inserting the paper layer and the lower hydrophilic film into the lower hydrophilic film and pressing and fixing the lower adhesive layer to the lower hydrophilic film using a pressing process.

At this time, step (a) includes forming the outer wall of the fluid passage and the detection area hydrophobic in the paper layer, as described above; Forming a hollow-paper passage by using a CO ₂ cutter at a portion spaced apart from the outer wall by a predetermined distance to the inside of the fluid passage; And impregnating the detection area with a reagent; . ≪ / RTI >

Particularly, the step of hydrophobicly forming the outer wall of the fluid passage and the detection region in the paper layer may be performed by photolithography, ink-jet, wax printing, impregnation & hardening, , Imprinting, and screen printing. [0033] The present invention is not limited to the above-described embodiments.

In addition, the step (b) may include cutting the upper adhesive layer to form a fluid inlet and a detection area.

The upper adhesive layer and the lower adhesive layer are made of a lamination film and can be thermocompression bonded at 70 to 130 ° C.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. Prior to this, terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms, and the inventor should appropriately interpret the concepts of the terms appropriately It should be construed in accordance with the meaning and concept consistent with the technical idea of the present invention based on the principle that it can be defined.

Therefore, the embodiments described in this specification and the configurations shown in the drawings are merely the most preferred embodiments of the present invention and do not represent all the technical ideas of the present invention. Therefore, It is to be understood that equivalents and modifications are possible.

FIG. 1 is a cross-sectional view of a paper pH sensor using the colorimetric method of the present invention, FIG. 2 is a cross-sectional view of the paper pH sensor using the colorimetric method of the present invention, FIG. 5 is an exploded perspective view of the paper pH sensor using the colorimetric method of the present invention, FIG. 6 is a diagram illustrating a process of manufacturing the paper pH sensor using the colorimetric method of the first embodiment of the present invention, FIG. 7 is a graph showing the contact angle change with respect to water according to the elapsed time after plasma treatment of the PET surface in Example 1 of the present invention. FIG. 8 is a graph showing the change of the contact angle with water according to the colorimetric method of the present invention FIG. 9 is an exploded perspective view of a paper pH sensor using the colorimetric method produced in Example 3 of the present invention, and FIG. 10 is a view showing an example of a paper color sensor using the colorimetric method prepared in Example 4 of the present invention. FIG. 11 is an exploded perspective view of the pH sensor using the colorimetric method manufactured in Example 5 of the present invention, and FIG. 12 is a view showing an exploded perspective view of the pH sensor obtained in Example 5 of the present invention. FIG. 13 is a graph showing the fluid movement speed for the fluid flow devices fabricated from the PET film without plasma treatment in Experimental Example 1 of the present invention, and FIG. 14 ((A) pH 5, (B) pH 7, (C) pH 8) showing the result of measuring the pH of a sample using the color pH sensor using the colorimetric method prepared in Example 1 of the present invention, Fig. 15 is a graph showing the results of measurement of the pH of a sample using the paper pH sensor using the colorimetric method prepared in Example 1 of the present invention, and Fig. 16 is a graph The pH of the side FIG. 17 is a graph showing the results of measurement of the pH of a sample using the paper pH sensor using the colorimetric method prepared in Example 2 of the present invention, and FIG. 18 is a graph showing the results of measurement FIG. 19 is a graph showing a result of measuring the pH of a sample using a color pH sensor using a colorimetric method, FIG. 19 is a graph showing the pH of a sample measured using a paper pH sensor using the colorimetric method prepared in Example 4 of the present invention FIG. 20 is a graph showing the results of measurement of the pH of a sample using a paper pH sensor using the colorimetric method manufactured in Example 5 of the present invention, and FIG. 21 is a graph showing the results of measurement FIG. 22 is a graph showing the result of measuring the pH of a sample using a paper pH sensor using a colorimetric method, and FIG. 22 is a graph showing the pH of a sample measured using a paper pH sensor using the colorimetric method prepared in Example 1 of the present invention. A view showing a result, 23 is a view showing a result of using a paper pH sensor using a colorimetric method of the present invention prepared in Example 1, measuring the pH of the sample.

Hereinafter, the paper pH sensor using the colorimetric method of the present invention will be described in detail with reference to FIGS. 1 to 23 and Examples.

The present invention utilizes a paper-based lab-on-a-chip to voluntarily and quickly move an aqueous solution sample to be tested for pH value, thereby quickly and accurately measuring a pH value to be measured To a paper pH sensor 100 using a colorimetric method.

1, the paper pH sensor 100 using the colorimetric method according to the present invention comprises a paper layer 110, an upper hydrophilic film 130 disposed on the upper and lower surfaces of the paper layer 110 and a lower hydrophilic film 140 ).

More specifically, the lower hydrophilic film 140, the paper layer 110 and the upper hydrophilic film 130 may be laminated in order to form a paper pH sensor 100, which is made of a hydrophobic member 111 And the inner side surface includes the fluid passage 112 made of the porous member 113, so that the aqueous solution sample can be spontaneously and quickly moved.

Here, the upper hydrophilic film 130 is disposed on the upper surface of the paper layer 110 and may include a fluid inlet 131 and / or a fluid outlet 132. In addition, the lower hydrophilic film 140 may be a hydrophilic material different from the paper layer 110.

In particular, in the paper pH sensor 100 of the present invention, the inner surface of the fluid passage 112 is made of the porous member 113, and the hollow-paper passage 115 can be formed. At this time, the hollow-paper passage 115 generates a capillary force by the porous member 113 formed on the paper layer 110 to generate a spontaneous fluid flow, and the resistance to flow due to the hollow 114 Can be minimized. A more detailed description will be given later.

FIG. 2 is a sectional view of a five-layered paper pH sensor 100 according to an embodiment of the present invention. As shown in FIG. 2, the colorimetric paper pH sensor 100 of the present invention has a top hydrophilic The film 130 and the lower hydrophilic film 140 are attached to the upper and lower surfaces of the paper layer 110 by an adhesive layer 150. At this time, at least one attachment may be made between the upper hydrophilic film 130 and the paper layer 110 or between the lower hydrophilic film 140 and the paper layer 110.

3 is an exploded perspective view of the paper pH sensor 100 of FIG. 2. As shown in FIG. 3, a fluid passage 112 is formed so that the adhesive layer 150 corresponds to the paper layer 110 .

At this time, the adhesive layer 150 may be a parafilm or an adhesive paste.

Particularly, the parafilm is excellent in durability against chemicals and can prevent the inflow of impurities into the channel, and the adhesive paste can be formed of acrylic resin or epoxy.

3, the paper layer 110 includes a fluid passage 112, and a plurality of detection areas 121 are formed along the fluid passage 112 And a detection unit 120 that is disposed. The hollow portion 114 may be formed of a hydrophobic member 111 except for the inner surface of the fluid passage 112 and the detection portion 120. The hollow portion 114 may be formed in the fluid passage 112 formed of the porous member 113, ), It is possible to move the aqueous solution sample voluntarily and quickly.

In the present invention, the detection unit 120 includes a plurality of detection areas 121, and the detection area 121 may be impregnated with each reagent that induces a color change at pH 0 to pH 14. [

For reference, the paper pH sensor 100 of the present invention may include the marking means 122 on the paper layer 110 or the upper hydrophilic film 130. The marking means 122 may be a scale capable of displaying the pH value of the analyte in the form of a scale similar to an alcohol thermometer or a check region 124 indicating the presence or absence of discoloration of the detection region 121.

Referring to FIG. 3, the paper pH sensor 100 of the present invention may include scales in the paper layer 110 with marking means 122.

4, in the paper pH sensor 100 of the present invention, the upper hydrophilic film 130 and the paper layer 110 are in close contact with each other by the intermediate adhesive layer 160, and the intermediate adhesive layer 160 may be formed with a space 161 to accommodate the paper layer 110 and the lower hydrophilic film 140. In addition, the lower hydrophilic film 140 may further include a lower adhesive layer 170 on the lower surface thereof. The intermediate adhesive layer 160 may include PDMS (polydimethylsiloxane), parafilm, and wax molecular sieve . ≪ / RTI > In such a case, a paper pH sensor 100 having a three-layer structure can be formed.

FIG. 5 is an exploded perspective view of the paper pH sensor 100 of FIG. 4, showing a laminated structure of a paper pH sensor 100 having a three-layer structure. The paper pH sensor 100 of the three-layer structure includes a paper layer 110 (see FIG. 1) including a hollow-paper path 115 and a hydrophobic member 111, and a plurality of detection areas 121 connected to the hollow- The upper hydrophilic film 130 and the lower hydrophilic film 140 can be closely fixed to the upper and lower portions of the paper layer 110 in a sandwich form. The intermediate adhesive layer 160 and the lower hydrophilic film 140 for supporting the paper layer 110 and the lower hydrophilic film 140 from the outside in order to closely contact the surfaces of the upper hydrophilic film 130 and the paper layer 110, And a lower adhesive layer 170 adhering the paper layer 110 and the lower hydrophilic film 140 while being adhered to the intermediate adhesive layer 160.

Particularly, the thickness of the paper layer 110 and the lower hydrophilic film 140 is greater than the thickness of the intermediate adhesive layer 160, so that the lower adhesive layer 170 and the lower hydrophilic film 140 can be tightly fixed.

In another aspect, the paper pH sensor 100 of the present invention includes a fluid passage 112 formed of a hollow-paper passage 115, and a plurality of detection areas 121 are formed in the longitudinal direction of the fluid passage 112 An upper adhesive layer 180 disposed on the upper surface of the paper layer 110 and having a fluid injection port 131 and / or a discharge port 132 formed thereon, And a lower adhesive layer 170 disposed on the lower surface of the lower hydrophilic film 140. The lower adhesive layer 170 may be disposed on the upper surface of the lower hydrophilic film 140,

At this time, the paper layer 110 and the lower hydrophilic film 140 are closely contacted with the upper adhesive layer 180, the paper layer 110 and the lower hydrophilic film 140 and the lower adhesive layer 170, The upper adhesive layer 180 and the lower adhesive layer 180, which are coated with an adhesive material on one side, are formed by inserting an intermediate layer 190 having an inner space 161 formed therein so as to receive the lower hydrophilic film 110 and the lower hydrophilic film 140, 170 are pressed to uniformly adhere the paper layer 110 and the lower hydrophilic film 140 to each other.

The upper adhesive layer 180 and the lower adhesive layer 170 may be a laminated film. For example, one side of the polyester film may be coated with an adhesive and thermally adhered at a temperature of 70 to 130 ° C. The inner paper layer can be firmly and easily attached by roll pressing at a temperature of about < RTI ID = 0.0 > < / RTI >

The reagent impregnated in the detection region 121 of the present invention may be a malachite, brilliant green, methyl green, methyl violet, crystal violet, Eosin bluish, ethyl violet, m-cresol purple, thymol blue, p-xylenol blue, 2,2 ' , 2 ", 4,4'-pentamethoxy-triphenylcarbinol, quinaldine red, 2,4-dinitrophenol (2,4-dinitrophenol), methyl yellow, bromochlorophenol, bromophenol blue, tetrabromophenol blue, congo red, methyl orange methyl orange, bromocresol green, 2,5-dinitrophenol, methyl red, chlorophenol red, Bromocresol purple, bromophenol red, nitrazine yellow, bromoxylenol blue, bromothymol blue (BTB), neutral red, phenol Phenol red, 3-nitrophenol, 1-naphtholphthalein, phenolphthalein, thymolphthalein, alizarin yellow GG, May be at least one selected from the group consisting of tropaeolin O, indigo carmine, epsilon blue, alkali blue and titan yellow.

3 and 5, the paper pH sensor 100 of the present invention may include a plurality of detection areas 121 along the longitudinal direction of the hollow-paper path 115, From one side to the other, each indicator that induces a color change from a lower pH value to a higher pH value can be impregnated in order.

For example, in the detection region 121, 10 kinds of pH indicators capable of detecting a sample having a pH value of 4 to 13 are added to the tetrabromophenol blue, , Bromocresol green, chlorophenol red, bromothymol blue, phenol red, phenolphthalein, thymolphthalein, phenolphthalein, And a solution of Titan yellow, a solution of tropaeolin O and a solution of Titan yellow. When a sample having a pH of 8 is injected into the injection port, the tetrabromo Detection with impregnated indicator of tetrabromophenol blue, bromocresol green, chlorophenol red, bromothymol blue, phenol red. The color of the station 121 is changed, it is possible to measure the pH of the sample.

In another embodiment, the reagent comprises an auxiliary compound capable of interacting with the at least one pH indicator described above and the indicator to change the value of the acid dissociation constant (pKa) of the indicator in an aqueous solution.

Here, the acid dissociation constant (pKa) means the equilibrium constant of the ionization equilibrium of the acid and the scale of the acid strength. The larger the value, the greater the ionization tendency.

More specifically, the introduction of the above-described auxiliary compound in the present invention is carried out by using one kind of indicator at a specific pH (pH) to minimize cognitive deterioration due to a variety of color fading occurring by using different pH indicators in the detection region 121 In order to induce the same color change in the section.

On the other hand, in order to induce a change in the discoloration pH value of the pH indicator, the auxiliary compound may be added to the indicator to cause the shift of the discoloration point. Since the same indicator is the same color, the discoloration pH is changed in a narrow range 1 to 2).

In general, pH indicators use ions that form ions through a proton dissociation reaction at certain pH values and that they exhibit a different color. Therefore, the auxiliary compounds capable of effectively changing the pKa value of the proton dissociation reaction are molecules capable of ion-ion or ion-dipole interaction.

Representative examples of the material include ionic surfactants such as cetyltrimethyl ammonium sulfate (CTAB), dodecyl pyridinium bromide, sodium dodecyl sulfate (SDS), sodium dodecylsulfonate (1,1,3,3-tetramethylbutyl) phenyl-polyethylene glycol (4- (1,1,3,3-tetramethylbutyl) phenyl) -polyethyleneglycol, which is a nonionic surfactant, and sodium dodecylsulfonate, sodium hexadecanoate, 3,3-Tetramethylbutyl) phenyl-polyethylene glycol (Trion X-100), and dodecyl penta (ethylene oxide).

In addition, ionic liquid compounds can also act as an auxiliary material, including trihexyl- (tetradecyl) phosphonium chloride, trimethylpyrazolium methylsulfate 1-ethyl-3-methylimidazolium chloride, trimethylpyrazolium methylsulfate, 1-butyl-3-methylimidazolium hydrogensulfate, .

On the other hand, the upper hydrophilic film 130 and / or the lower hydrophilic film 140 are formed of a transparent substrate, so that the user can easily observe the changed color of the detection region 121.

In addition, the upper hydrophilic film 130 and the lower hydrophilic film 140 may be formed of a polymer film such as polyethylene terephthalate (PET), polystyrene (PS), polymethylmethacrylate (PMMA), polyester (PES, Polyester), polyethylene (PE, Polyethylen) and cyclic olefin copolymers (COC).

In one embodiment, the polymer film may be a polymer film surface-treated with plasma. That is, the hydrophobic polymer film may be plasma-treated to have hydrophilicity.

For reference, the polymer films can also induce spontaneous fluid flow effectively by introducing an oxidizing functional group into a hydrophilic surface by plasma treatment, and can improve the hydrophilicity of the polymer film through other chemical and physical methods.

In addition, the present invention relates to a method for producing a paper pH sensor 100 using a colorimetric method.

(B) producing an upper hydrophilic film 130 and a lower hydrophilic film 140; and (c) forming a lower hydrophilic film 140. The method further comprises the steps of: (a) ) The upper and lower hydrophilic films and the lower hydrophilic film 140 are disposed on the upper and lower surfaces of the paper layer, and are fixed using adhesion or adhesion pressing processes.

The step (a) may include forming the outer wall of the fluid passage 112 and the detection area 121 in the paper layer 110 in a hydrophobic manner, separating the inner wall of the fluid passage 112 from the outer wall at a predetermined interval Forming a hollow-paper passage 115 by using a CO ₂ cutter in the exposed area and impregnating the indicator area 121 with an indicator.

Particularly, the step of forming the outer wall of the fluid passage 112 and the detection area 121 in the paper layer 110 by hydrophobic may be performed by photolithography using a SU-8 photosensitive polymer material and a semiconductor process, Wax printing to eject the hydrophobic solid wax, impregnation & hardening to penetrate and harden the hydrophobic material into the paper and cure in a selective area, Various methods such as imprinting, plasma processing, screen printing, and the like for transferring a hydrophobic substance to a pattern tension can be applied.

In the following embodiments, a pattern is formed using a wax printer, but the present invention is not limited thereto, and it is natural that a wax printing technique according to the prior art can be used. That is, without using a wax printer, a mask having a pattern corresponding to a pattern can be covered on the paper, and the wax can be treated to wax-print the pattern. At this time, the wax can be printed on the mask in a solid state as it is, or it may be heated by a temperature above the melting point and applied with a brush or the like.

In one embodiment, the width of the fluid passage may vary depending on the amount of fluid used, the size of the element, and the like, and may have a width of about 1 to 10 mm. In addition, it is preferable that the fluid passage 112 symmetrically leaves a predetermined partial paper on both outer walls. The hollow-aeration passage may serve to connect a plurality of detection regions 121 present in the paper layer 110 to an introduction portion of the aqueous solution sample. Meanwhile, the detection area 121 may include at least one connection part 123 to be connected to the paper layer 110. More specifically, when the hollow-paper path 115 is cut by using a CO ₂ laser cutter, cutting is performed except for the connecting portion 123, the detecting portion 120 is cut off, To the paper layer 110.

The step (b) includes a step of cutting the upper hydrophilic film 130 to form a fluid inlet 131 and a detection area 121, and the three-layer structure paper shown in FIGS. 4 and 5 the step (c) of attaching the paper layer 110 between the upper hydrophilic film 130 and the lower hydrophilic film 140 using the adhesive layer 150 and the step of bonding the adhesive layer 150 to the lower hydrophilic film 140, 150, the lower hydrophilic film 140, and the paper layer 110, which are adhered by the upper hydrophilic film 130, the lower hydrophilic film 140, and the paper layer 110, respectively.

Particularly, in the case of the parafilm, when the pressure is applied at room temperature, it may be possible to adhere while modifying if a certain pressure is applied even if heat is not applied. The pressure at this time may be ² kgf / cm ² to 100 kgf / cm ² . For example, a parafilm can be used as the adhesive layer 150 of the present invention, and such a parafilm may undergo denaturation by pressurization even at room temperature. On the other hand, when thermocompression proceeds, it may occur in the range of 45 to 95 ° C. For reference, the heat of the parafilm is thermally deformed from a temperature near 45 ° C, and the heat distortion occurs too much at a high temperature, resulting in a collapse of the pattern shape. In addition, the parafilm can be easily processed in the same pattern as the channel pattern by using a laser cutter, and is excellent in durability against chemicals, so that the impurity can be prevented from flowing into the fluid passage 112. For example, Lt; RTI ID = 0.0 > 60 C < / RTI >

On the other hand, the paper pH sensor 100 including the hollow-paper passage 115 is less likely to leak than a polydimethylsiloxane (PDMS) element that generates a relatively high pressure due to a very low pressure applied to the fluid, It does not have to be high. As another alternative, there is a method of screen-bonding the adhesive paste to directly form the patterned adhesive layer 150 on the hydrophilic film, followed by adhering to the paper layer 110.

4 and 5, the step (c) includes the steps of forming a paper sheet in the intermediate adhesive layer 160 in which the space 161 is formed, Inserting the layer 110 and the lower hydrophilic film 140 and pressing the lower adhesive layer 170 under the lower hydrophilic film 140 by a pressing process; . ≪ / RTI >

At this time, the lower adhesive layer 170 uses a lamination film and can be thermocompression bonded at 70 to 130 ° C, specifically 90 to 110 ° C.

In another aspect, a method of manufacturing a paper pH sensor using the colorimetric method of the present invention includes the steps of (a) preparing a paper layer including a hollow-paper path, (b) preparing a top adhesive layer 180 and a bottom adhesive layer (C) disposing the upper adhesive layer (180) and the lower adhesive layer on the upper and lower surfaces of the paper layer, and fixing the upper adhesive layer (180) and the lower adhesive layer using a pressing process of adhering; Inserting the paper layer and the lower hydrophilic film into the formed intermediate layer 190, and fixing the lower adhesive layer 170 to the lower hydrophilic film using a pressing process.

At this time, the step of manufacturing the paper layer is as described above, so a detailed description thereof will be omitted.

Meanwhile, the upper adhesive layer 180 may be cut to form a fluid inlet and an outlet. In particular, the outlet may correspond to the position of the detection area of the paper layer.

At this time, the lower adhesive layer 170 uses a lamination film and can be thermocompression bonded at 70 to 130 ° C, specifically 90 to 110 ° C.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail with reference to the following examples. However, the following examples are intended to illustrate the contents of the present invention, but the scope of the present invention is not limited to the following examples. Embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art.

≪ Example 1 > Preparation of Paper pH Sensor Using Adhesion Method

Example 1-1. The reagent used in the detection area

The pH indicators which can be used in a plurality of detection regions included in the detection unit are shown in Table 1 according to the pH value of one unit.

In order to induce effective discoloration at an initial indicator color and a given pH value, a buffer solution in which an indicator according to each pH value was dissolved was impregnated in the detection region 121 and used. The specific pH buffer solution preparation method is summarized in Table 2 below. For reference, the reagents used in the preparation of the buffer solution samples listed in Table 2 were purchased from Sigma Aldrich.

pH Available pH indicator reagent One Malachite green oxalate, Brilliant green, Methyl green, Methyl violet, Crystal violet 2 Eosin bluish, Ethyl violet, m-Cresol purple, Thymol blue, p-Xylenol blue, 2,2 ', 2 ", 4,4'-pentamethoxytriphenylcarbinol 3 Quinaldine red, 2,4-Dinitrophenol, Methyl yellow 4 Bromochlorophenol, Bromophenol blue, Tetrabromophenol blue, Congo red, Methyl orange 5 Bromocresol green, 2,5-Dinitrophenol, Methyl red 6 Chlorophenol red, Bromocresol purple, Bromophenol red, Natrazine yellow 7 Bromoxylenol blue, Bromthymol blue, Neutral red 8 Phenol red, 3-Nitrophenol, 1-Naphthophthalein, m-Cresol purple 9 Thymol blue, p-Xylenol blue, Phenolphthalein 10 Thymolphthalein 11 Alizarin yellow GG 12 Tropaeolin O, Indigo carmine, Epsilon blue, Alkali blue 13 Titan yellow

pH Chemical composition Manufacturing method 1.0 HCl / KCl 0.2 M KCl 25 mL + 0.2 M HCl 67 mL 2.0 HCl / KCl 0.2 M KCl 25 mL + 0.2 M HCl 6.5 mL 3.0 Acetate buffer 0.1 M acetic acid 19.65 mL + 0.1 M sodium acetate 0.35 mL 4.0 Acetate buffer 0.1 M acetic acid 16.94 mL + 0.1 M sodium acetate 3.06 mL 5.0 Acetate buffer 0.1 M acetic acid 7.14 mL + 0.1 M sodium acetate 12.86 mL 6.0 Phosphate buffer 1.0 MK ₂ HPO ₄ 2.64 mL + 1.0 MK ₂ H ₂ PO ₄ 17.36 mL 7.0 Phosphate buffer 1.0 MK ₂ HPO ₄ 12.3 mL + 1.0 M KH ₂ PO ₄ 7.7 mL 8.0 Phosphate buffer 1.0 MK ₂ HPO ₄ 18.8 mL + 1.0 M KH ₂ PO ₄ 1.2 mL 9.0 Phosphate buffer 1.0 MK ₂ HPO ₄ 5.0 mL + 1.0 M KH ₂ PO ₄ 0.2 mL + 1.0 M K ₃ PO ₄ 0.5 mL 10.0 NaHCO ₃ + NaOH 0.05 M NaHCO ₃ 50 mL + 0.1 M NaOH 10.7 mL 11.0 NaHCO ₃ + NaOH 0.05 M NaHCO ₃ 50 mL + 0.1 M NaOH 22.7 mL 12.0 KCl + NaOH 0.2 M KCl 50 mL + 0.2 M NaOH 12.0 mL 13.0 KCl + NaOH 0.2 M KCl 25 mL + 0.2 M NaOH 66.0 mL

Example  1-2. Hollow-paper path formed Paper layer  Produce

In this embodiment, a paper layer 110 comprising a fluid passage 112 comprised of a hollow 114 and a porous member 113 was prepared.

6, a plurality of fluid passages 112 extend radially from the fluid inlet 131 at the central portion in this embodiment, and the ends of the fluid passages 112 extend radially from the center of the paper layer (110).

In this embodiment, cellulose paper (Whatman, Chromatography Paper grade # 1) having a thickness of about 160 탆, which is commercially available for cellulose chromatography, is used as the paper layer 110, which is 50 × 50 × 0.5 mm ³ (width × length × thickness). The cellulose paper used here is composed of cellulose molecular sieve and has a porosity of about 60 to 75%.

6 (A), the fluid passage 112 is designed so that the fluid flow distance is 10 mm, and the fluid passage 112 and the predetermined portion used as the detection region 121 on the paper layer 110 The solid wax was transferred. At this time, the width of each of the fluid passages 112 was 3 mm, and the fluid movement distance from the fluid injection port to the detection region 121 was 10 mm. The detection area 121 is connected to the fluid passage 112 and formed into a circle having a diameter of 8 mm. For reference, in this embodiment, six fluid passages 112 and a detection region 121 are provided.

Next, a wax existing on the surface was infiltrated into the paper layer 110 using a subsequent heat treatment process to form a hydrophobic fluid flow outer wall in the direction of penetrating the paper. Typical subsequent heat treatment process conditions were 1 minute on a 130 ° C hotplate.

The hollow-paper path 115 was formed in the paper layer 110 formed by the wax printing / heat treatment process using an additional cutting process. More specifically, it was subjected to the cutting step by using a CO ₂ cutters, as shown in Figure 6 (B), and the width of the fluid passage (112) 3mm, paper fulfill this width by removing a 1mm 1mm in its center The layer 110 was cut to form a hollow-paper path 115.

In addition, the outer wall of the detection area 121 was cut using a CO ₂ cutter like the hollow-paper path 115, except that the connection part 123 of 1 mm x 1 mm was cut, The detection area 121 and the paper layer 110 can be connected to each other using the light guide 123.

Examples 1-3. Detection region formation

In this embodiment, the detection area 121 of the paper layer 110 produced in Example 1-2 was impregnated with an indicator.

Six detection areas 121 are formed in the embodiment 1-2. In this embodiment, the six detection areas 121 are referred to as a first detection area 1211, a second detection area 1212 ), A third detection area 1213, a fourth detection area 1214, a fifth detection area 1215, and a sixth detection area 1216, respectively.

First, a bromocresol green ethanol solution (1 g / 100 mL) is applied to the second detection region 1212 and a methyl red (Sigma Aldrich) ethanol solution Phenol red (Sigma Aldrich) was added to the fourth detection region 1214, bromothymol blue ethanol solution (1 g / 100 mL) was added to the fourth detection region 1214, and phenol red (Sigma Aldrich) 2 μl of phenolphthalein ethanol solution (1 g / 100 ml) was impregnated into the ethanol solution (0.1 g / 100 ml) and the sixth detection area 1216, followed by drying at room temperature.

Examples 1-4. Production of upper and lower hydrophilic films

In this embodiment, polyethylene terephthalate (PET) having a thickness of 100 탆 was used as the upper hydrophilic film 130 and the lower hydrophilic film 140. More specifically, the polyethylene terephthalate used as the upper hydrophilic film 130 was cut to an appropriate size, and then a circular fluid inlet 131 and an outlet 132 were formed to inject and discharge the solution. At this time, the injection port was formed at a position corresponding to the injection port formed in the paper layer 110, and the discharge port 132 was formed at a position corresponding to the detection area 121, respectively. At this time, the injection port and the discharge port 132 were performed through a laser cutting process.

Then, the surface was modified with oxygen plasma to make the polyethylene terephthalate used as the upper hydrophilic film 130 and the lower hydrophilic film 140 hydrophilic.

FIG. 7 is a graph showing the change in contact angle with water with elapsed time after plasma treatment of the surface of polyethylene terephthalate. FIG. Polyethylene terephthalate with an initial contact angle of around 70 degrees had a value close to 0 immediately after the plasma treatment and a sharp increase in the contact angle for the next 12 hours. And then stabilized at around 50 ° C. The polyethylene terephthalate film was used in the manufacture of the fluid flow device of the present invention after being stored in the atmospheric condition for 12 hours after the plasma treatment since the contact angle was severely changed and the reproducibility of the fluid flow was deteriorated.

Examples 1-5. Thermocompression process

In this embodiment, parafilm M is used as the adhesive layer 150 in order to easily adhere the upper and lower hydrophilic films 130 and 140 to the upper and lower surfaces of the paper layer 110. At this time, the parafilm for adhering the upper hydrophilic film 130 and the paper layer 110 was processed into the same pattern as the pattern of the fluid passage 112 by using a laser cutter.

The lower hydrophilic film 140, the adhesive layer 150, the paper layer 110, the adhesive layer 150, and the upper hydrophilic film 130 were laminated in this order, and the thermocompression bonding process was performed.

More specifically, the thermocompression bonding process was performed on a hot plate, and thermocompression was performed at a temperature of 75 ° C. at a pressure of 1.0 kgf / cm ² or less to manufacture the paper pH sensor 100 using the colorimetric method of the present invention Respectively.

≪ Example 2 > Preparation of Paper pH Sensor Using Adhesion Method

In this embodiment, the paper pH sensor 100 shown in Fig. 8 was manufactured.

More specifically, the paper layer 110 was made of cellulose paper (Whatman, Chromatography paper grade # 1) having a 160 μm thickness commercially available for chromatography, which was 25 × 75 × 2 mm ³ (width × length × thickness ), And the fluid flow distance was designed to be approximately 50 mm.

First, the distance of the fluid flow was designed to be 50 mm. On the paper layer 110, the hollow-paper path 115 was formed by using the same method as in Example 1-2, namely, wax printing and laser cutting to form a hydrophobic barrier And a cutting process was performed.

More specifically, referring to Fig. 8, wax printing is performed except for the fluid passage 112 extending in the longitudinal direction, the plurality of detection areas 121, the connection part 123, and the control area 124. [ More specifically, the fluid passage 112 was 4 mm wide, the distance was 50 mm, and the detection area 121 and the check area 124 were wax-printed with a diameter of 3 mm. The connection portion 123 connecting the fluid passage 112 and the detection region 121 has a size of 2 mm x 1 mm. Thereafter, the fluid inlet 131 and the fluid passage 112 were cut using a CO ₂ cutter to form the hollow-paper path 115. The hollow-paper passage 115 at this time has a width of 2 mm and a length of 50 mm.

Then, 10 kinds of pH indicators capable of detecting a sample having a pH value ranging from 4 to 13 in the detection region 121 and the check region 124 were placed in the order of lower pH value in the order of lower Tetrabromophenol It is also possible to use blue (tetrabromophenol blue, Sigma Aldrich), bromocresol green (bromide), chlorophenol red (Sigma Aldrich), bromothymol blue red, JUNSEI chemical, phenolphthalein, thymolphthalein (Sigma Aldrich), a mixed solution of phenolphthalein and titan yellow (TCI), tropaeolin O (TCI) The titanium-yellow solution was dispensed and dried.

For reference, the pH-indicating reagent can be replaced with another pH-indicating reagent having a similar color change range shown in [Table 1], and the initial pH can be adjusted with a buffer solution.

The upper hydrophilic film 130 is formed on the paper layer 110 with a laser cutter and the lower hydrophilic film 140 and the adhesive layer 150 are made of a commercially available box tape A fluid injection port 131 having the same size as that of one fluid injection port 131 was formed.

Thereafter, the lower hydrophilic film 140, the adhesive layer 150, the paper layer 110, the adhesive layer 150, and the upper hydrophilic film 130 are laminated in this order, and the thermocompression bonding process is performed at a temperature of 60 ° C, The paper pH sensor 100 shown in Fig.

≪ Example 3 > Preparation of Paper pH Sensor Using Adhesion Method

In this embodiment, the paper pH sensor 100 shown in Fig. 9 was manufactured.

More specifically, the paper layer 110 was made of cellulose paper (Whatman, Chromatography paper grade # 1) having a thickness of 160 탆, which is commercially available for chromatography, which is 25 × 75 × 2 mm ³ (width × length × thickness ).

First, the distance of the fluid flow was designed to be 50 mm. On the paper layer 110, the hollow-paper path 115 was formed by forming a hydrophobic barrier using wax printing, which is the same method as in Example 1-2, The cutting process was performed using CO ₂ laser cutting.

More specifically, referring to Fig. 9, wax printing is performed except for the fluid passage 112, the plurality of detection areas 121, and the connection part 123 extending in the longitudinal direction. More specifically, the fluid passage 112 has a width of 3 mm, a distance of 50 mm, a detection area 121 of 3 mm x 2 mm, and a connection part 123 of 3 mm x 1 mm, 121, and the connecting portion 123 were removed. Thereafter, the fluid inlet 131 and the fluid passage 112 were cut using a CO ₂ cutter to form the hollow-paper path 115. The hollow-paper passage 115 at this time has a width of 1 mm and a length of 50 mm.

At this time, the detection area 121 has 14 pieces. In the detection area 121 corresponding to the range of pH 1 to 13, 14 kinds of pH indicators capable of detecting the sample having the pH value are arranged in the order of low pH value (TCI), ethyl violet (TCI), quinaldine red (TCI), tetrabromophenol blue (Sigma Aldrich), bromocresol green bromocresol green, and purified water), chlorophenol red (Sigma Aldrich), bromothymol blue (phenol red), phenol red (JUNSEI chemical), phenolphthalein, (TCI), Thymolphthalein (Sigma Aldrich), a mixed solution of Phenolphthalein and Titan yellow (TCI), tropaeolin O (TCI), and Titan yellow When the reagent is dispensed and dried The.

A marking means 122 such as a scale is provided on each of the detection regions 121 adjacent to the detection region 121. Each detection region 121 has a detection pH value ranging from pH 1 to pH 13 Is indicated by marking means (122). The marking means 122 may be provided on the upper hydrophilic film 130.

For reference, the pH-indicating reagent can be replaced with another pH-indicating reagent having a similar color change range shown in [Table 1], and the initial pH can be adjusted with a buffer solution.

A polyethyleneterephthalate film (100 탆 thick, SKC) was used as the upper hydrophilic film 130 and the lower hydrophilic film 140, and a parafilm M was used as the adhesive layer 150.

At this time, the upper hydrophilic film 130 formed a fluid injection port 131 having the same size as the fluid injection port 131 formed in the paper layer 110 with a laser cutter.

Thereafter, the lower hydrophilic film 140, the adhesive layer 150, the paper layer 110, the adhesive layer 150, and the upper hydrophilic film 130 are laminated in this order, and the thermocompression bonding process is performed at a temperature of 60 ° C, The paper pH sensor 100 shown in Fig.

<Example 4> Manufacture of paper pH sensor using tightly fixing method

In this embodiment, the three-layered paper pH sensor 100 shown in Fig. 10 was manufactured.

Here, the three-layered paper pH sensor 100 refers to a paper sensor in which the upper hydrophilic film and the paper layer 110 are in close contact with each other by an intermediate adhesive layer, and the intermediate adhesive layer includes a paper layer 110 and a lower hydrophilic film 140 And a space 161 is formed so as to be able to receive the pH sensor.

In the present embodiment, the paper layer 110 is made of cellulose paper (Whatman, Chromatography paper grade # 1) having a thickness of 160 탆, and the overall paper pH sensor 100 is approximately 25 × 75 × 2 mm ³ And a fluid passage 112 is formed in the central portion so that the two fluid flows at a distance of 25 mm on one side and the other side of the fluid passage injection port.

Next, a hydrophobic barrier was formed by using the same method as that performed in the above Example 1-2, using wax printing, and a hollow-paper path 115 was formed by laser cutting.

Referring to FIG. 10, the pH sensor of the present embodiment is wax-printed except for the fluid passage 112 extending in the longitudinal direction, the plurality of detection areas 121, and the connection part 123. More specifically, the fluid passage 112 has a width of 3 mm, a distance of 50 mm, a detection area 121 of 3 mm x 2 mm, and a connection part 123 of 3 mm x 1 mm, 121, and the connecting portion 123 were removed. Thereafter, the fluid inlet 131 and the fluid passage 112 were cut using a CO ₂ cutter to form the hollow-paper path 115. The hollow-paper path 115 at this time has a width of 1 mm and a length of 25 mm on both sides of the fluid injection port 131, respectively.

In the detection region 121 corresponding to the range from pH 1 to pH 13, 14 pH indicators capable of detecting a sample having a pH value are arranged in the order of low pH value (TCI), ethyl violet (TCI), quinaldine red (TCI), tetrabromophenol blue (Sigma Aldrich), bromocresol green bromocresol green, and purified water), chlorophenol red (Sigma Aldrich), bromothymol blue (phenol red), phenol red (JUNSEI chemical), phenolphthalein, , Tymolphthalein (Sigma Aldrich), a mixed solution of Phenolphthalein and Titan yellow (TCI), tropaeolin O (TCI), Titan yellow (TCI) ) PH indicator was used All.

A marking means 122 such as a scale is provided on each of the detection regions 121 adjacent to the detection region 121. Each detection region 121 has a detection pH value ranging from pH 1 to pH 13 Is indicated by marking means (122). The marking means 122 may be provided on the upper hydrophilic film 130.

For reference, the pH indicator may be replaced with another pH indicator reagent having a similar hue change range shown in Table 1, and the initial pH can be adjusted with a buffer solution.

In order to make the upper hydrophilic film and the paper layer 110 come into direct contact with each other, the upper hydrophilic film has the same size as the upper hydrophilic film and has the same size as the paper layer 110 161 were formed. In the present embodiment, the intermediate adhesive layer 160 may be a composite adhesive sheet prepared by infiltrating the adhesive molecular sieve into a porous member 113 such as paper, or a commercial double-sided tape having adhesive components on both sides. The paper layer 110 and the lower hydrophilic film 140 are inserted into the intermediate adhesive layer 160 after the intermediate adhesive layer 160 is fixed to the upper hydrophilic film 130 by thermocompression at 75 ° C., The lower adhesive layer 170 may be aligned and fixed by a pressing process. Specifically, a lamination film was used as the lower adhesive layer 170, and the laminated film was adhered to the intermediate adhesive layer 160 and the upper hydrophilic film 130 by a thermal pressing (lamination) process at 100 ° C.

The paper pH sensor 100 of the present invention was manufactured by using the adhesion fixing method.

In order to firmly adhere the hydrophilic film and the paper layer 110 in this way, it is preferable that the sum of the thicknesses of the middle layer and the lower hydrophilic film 140 is larger than the middle adhesive layer 160 by several tens of micrometers.

Example 5: Preparation of paper pH sensor

In this embodiment, a paper pH sensor having the three-layer structure shown in Fig. 11 was manufactured.

The paper pH sensor of the present embodiment has a three-layer structure composed of an upper adhesive layer 180, an intermediate layer 190 containing a paper layer and a lower hydrophilic film, and a lower adhesive layer 170, Means a pH sensor to be formed.

In the present embodiment, the paper layer was made of cellulose paper (Whatman, Chromatography paper grade # 1) having a thickness of 160 μm, and the overall paper pH sensor had a size of about 25 × 75 × 2 mm ³ (width × length × thickness) And a fluid passage is formed in the central portion so that the two fluid flows at a distance of 25 mm on one side and the other side of the fluid passage injection port.

Next, a hydrophobic barrier was formed using wax printing, which was the same method as in Example 1-2, and a hollow-paper path was formed by laser cutting.

Referring to FIG. 11, the pH sensor of this embodiment is wax-printed except for a fluid passage extending in the longitudinal direction, a plurality of detection areas, and a connection portion 123. More specifically, the fluid passage was wax-printed except for a fluid passage, a detection area, and a connection portion so as to have a width of 3 mm, a distance of 50 mm, a detection area of 3 mm x 2 mm, and a connection area of 3 m x 1 m. The fluid inlet and fluid passages were then cut using a CO ₂ cutter to form a hollow-paper path. At this time, the hollow-paper passage has a width of 1 mm and a length of 25 mm on both sides of the fluid injection port.

In the detection range corresponding to pH 1 to pH 13, 14 pH indicators capable of detecting a sample having a pH value were arranged in the order of lower pH value from the lower side to the upper side in the order of crystal violet (TCI), ethyl violet (TCI), quinaldine red (TCI), tetrabromophenol blue (Sigma Aldrich), bromocresol green ), Mixed solution of bromocresol purple (Sigma Aldrich), cetyltriammonium bromide (Sigma Aldrich), bromothymol blue (Samseon Pure Chemical Industries) and cetyltri ammonium bromide, thymol blue thymol blue, thymolphthalein (Sigma Aldrich), a mixed solution of thymol blue and titan yellow (TCI), a mixture of thymol blue and tetramethylammonium bromide, , Tropez De O (tropaeolin O, TCI), was used as a titanium yellow pH indicator.

Marking means such as scales were prepared by using a wax printing method on each of the detection regions, and the detection pH values of the respective detection regions from pH 1 to pH 13 were indicated by markers. Such marking means may be provided in the upper adhesive layer 180.

For reference, the pH indicator may be replaced with another pH indicator reagent having a similar hue change range as shown in Table 1, the initial pH can be adjusted with the buffer solution, and the pH can be adjusted The pH can be measured more precisely by adding an auxiliary compound.

Meanwhile, in this example, a cationic surfactant, cetyltriammonium bromide, was added to bromocresol purple, bromothymol blue and thymol blue to prepare a pH indicator reagent, So that color change can occur at a pH lower than the pH causing the change.

An intermediate layer 190 having the same size as the upper adhesive layer 180 and having the same size as the paper layer so as to accommodate the paper layer is formed in order to contact the upper adhesive layer 180 and the paper layer in direct contact with each other. Were prepared.

In this embodiment, the upper adhesive layer 180 and the lower adhesive layer 170 are laminated films coated with an adhesive. In order to complete the pH sensor of the present invention, the intermediate layer 190 is aligned on the upper adhesive film, and after the lower adhesive layer 170 (lamination film) is aligned after inserting the intermediate layer 190 in the paper layer- The layers were bonded together by a 100 占 폚 hot pressing (lamination) process. In this case, in order to firmly adhere the hydrophilic film to the paper layer, it is preferable that the thickness of the paper layer-lower hydrophilic film is thicker than that of the intermediate layer 190 by several tens of micrometers.

<Experimental Example 1>

Experimental Example 1-1. Measuring Fluid Movement Speed - 1

In order to investigate the influence of the pH paper sensor including the hollow-paper path 115 on the flow of fluids in this experimental example, three kinds of fluid flow devices were prepared.

More specifically, using a fluid flow device comprising a hydrophilic paper layer with a 2 mm hollow, a paper layer with 2 mm hollow and 2 mm porous material, or a paper layer with 1 mm hollow and 1 mm porous material formed, The moving distance was measured.

For reference, a hydrophobic paper layer having a hollow of 2 mm means a paper layer not containing a porous material, and a paper layer having a hollow of 2 mm and a porous material of 2 mm is formed on both sides of a hollow 2 mm Means a paper layer containing a porous material having a width of 1 mm. In addition, a paper layer having a hollow of 1 mm and a porous material of 1 mm means a paper containing porous material of 0.5 mm width on both side walls of 1 mm hollow.

In this experiment, 300 쨉 g of ink was dropped on the fluid injection port of each of the three kinds of fluid flow devices, and the moving distance of the ink obtained during the movement of 100 mm was measured. The results are shown in Fig.

Referring to Fig. 12, when the ink moves 100 mm, the fluid flow element including the hydrophobic paper layer with the 2 mm hollow is formed in the fluid flow element 2 having the paper layer formed with the porous material of 2 mm hollow and 2 mm for 40 seconds, Lt; RTI ID = 0.0 &gt; 25 sec, &lt; / RTI &gt; a fluid flow element comprising a layer of paper formed with 1 mm hollow and 1 mm porous material took 15 seconds.

In this experiment, it was found that the fluid flow element including the hollow-paper passage was faster than the fluid flow element including only the hollow, and the width of the hollow-paper passage was larger than that of the hollow- It was confirmed that the flow velocity of the fluid flow device including the narrow paper layer shows a higher flow velocity.

This is because in the experiment in which the initial fluid pressure was controlled to zero, the fluid flow element including only the hollow does not generate spontaneous flow, but the fluid flow element including the hollow-paper path shows that the fluid can move along the path by itself .

In other words, by applying the fluid flow device including the hollow-paper passage in the present experiment to the pH paper sensor of the present invention, it is possible to speed up the flow of the fluid and quickly observe the color change of the indicator impregnated in the pH paper sensor .

Experimental Example 1-2. Measuring Fluid Movement Speed - 2

13 is a graph showing a fluid movement distance with time using three types of fluid flow devices made of a non-plasma treated polyethylene terephthalate film.

The same tendency as in Experimental Example 1-1 was observed when polyethylene terephthalate without surface modification using plasma was used.

However, in the fluid flow device manufactured using the polyethylene terephthalate film without plasma treatment, since the film surface has more hydrophobic property, it is possible to prevent the later fluid flow Speed. This indicates that the upper and lower film surfaces must have high hydrophilicity to induce spontaneous flow.

< Experimental Example  2> pH measurement using paper pH sensor

In the present experimental example, the pH of the sample was measured using the paper pH sensor 100 manufactured in Example 1

More specifically, a buffer solution sample having pHs of 5, 7 and 8, respectively, is injected into the fluid injection port 131 located at the center of the paper pH sensor 100 prepared in Example 1, and the color The difference in change was observed, and the results are shown in Fig. For reference, as the buffer solutions having the respective pHs 5, 7 and 8, the solutions described in Table 2 of Example 1-1 were used and the buffer solutions corresponding to the corresponding pH values were used.

14, when a buffer solution sample having a pH of 5 was injected into the fluid injection port 131, only the color of the second detection means impregnated with the bromocresol green solution changed, A second detection means in which a solution of bromocresol green, methyl red and bromothymol blue is respectively impregnated when the buffer solution sample having the first buffer solution is injected into the fluid injection port 131, 3 detection means and the fourth detection means are changed in color. When a buffer solution sample having pH 9 is injected into the fluid injection port 131, bromocresol green, methyl red, bromothymol blue, and phenol red (See FIGS. 14 (A), (B) and (C)). The color of the second detecting means, the third detecting means, and the fourth detecting means, respectively,

&Lt; Comparative Example 1 > Hollow-paper with paper path formed pH sensor and paper with paper path formed pH measurement using pH sensor

In this comparative example, pH measurement was performed using a paper pH sensor 100 in which a hollow-paper path 115 was formed and a paper pH sensor 100 in which a paper path was formed, and the results were compared.

Here, the paper pH sensor 100 in which the hollow-paper path 115 is formed refers to the paper pH sensor 100 manufactured in Example 1, and the paper pH sensor 100 in which the paper path is formed is referred to as a By using a CO ₂ laser cutter in Example 1, it means a form before the hollow-paper path 115 is formed. That is, it means a pH sensor in which no hollow-paper passage 115 is formed in the fluid passage 112.

First, a buffer solution sample having a pH of 9 was injected into a fluid inlet 131 of two pH sensors, and bromocresol green, methyl red, bromothymol blue, phenol The color of the second to sixth detecting means impregnated with red (phenol red) and phenolphthalein dl changed.

15 and 16, the detection means of the paper pH sensor 100 'in which the color of the detection means is uniformly changed, but the paper pH sensor 100' in which the paper path is formed, The color did not change uniformly.

In addition, the paper pH sensor 100 in which the hollow-paper path 115 is formed takes one second to inject the sample and change the color of the detection means. However, the paper pH sensor having the paper path formed therein injects the sample, It took 96 seconds to change color.

That is, since the color of the detection area 121 is uniformly changed by forming the hollow-paper path 115, the paper pH sensor 100 of the present invention can more easily judge the color change with accurate color, Since the flow velocity of the fluid was faster than the pH at which the paper passage was formed, the pH of the sample could be measured quickly.

< Experimental Example  3> pH measurement using paper pH sensor

In this experiment, the pH of the sample was measured using the paper pH sensor prepared in Example 2

More specifically, a buffer solution sample having pH 13 was injected into the fluid injection port 131 of the paper pH sensor 100 prepared in Example 2, and the difference in color change occurring in each detection means was observed, Is shown in Fig. As a reference, the buffer solution having the pH 13 described above was used as the buffer solution of the corresponding pH shown in Table 2 in Example 1-1. That is, a buffer solution of KCl + NaOH having a pH of 13 was used as the buffer solution used in the experimental example of the present invention.

Referring to FIG. 17, the pH indicators tetrabromophenol blue, bromocresol green, chlorophenol red, bromothymol blue, phenol red, , A mixed solution of phenolphthalein, thymolphthalein, phenolphthalein and titanium yellow, a detection zone impregnated with tropaeolin O, a titanium yellow solution ( 121) were changed in color.

In particular, the pH paper sensor of FIG. 17 has a check area 124 in which the hue does not change so as to correspond to the detection area 121, and it is possible to more easily confirm whether or not the hue is changed.

< Experimental Example  4> pH measurement using paper pH sensor

In the present experimental example, the pH of the sample was measured using the paper pH sensor 100 prepared in Example 3

More specifically, a buffer solution sample having a pH of 13 was injected into the fluid injection port 131 of the paper pH sensor 100 produced in Example 3, and the difference in color change occurring in each detection means was observed, The results are shown in Fig. As a reference, the buffer solution having the pH 13 described above was used as the buffer solution of the corresponding pH shown in Table 2 in Example 1-1. That is, a buffer solution of KCl + NaOH having a pH of 13 was used as the buffer solution used in the experimental example of the present invention.

Referring to FIG. 18, the pH indicator may be selected from the group consisting of crystal violet, ethyl violet, quinaldine Red, tetrabromophenol blue, bromocresol green, A mixed solution of phenol red, chlorophenol red, bromothymol blue, phenol red, phenolphthalein, thymolphthalein, phenolphthalein and titanium yellow, The color of the detection region 121 impregnated with tropaeolin O and titan yellow was changed.

In particular, in the pH paper sensor of FIG. 18, the scale and the pH of the indicator impregnated in the detection area 121 are displayed in the vicinity of the detection area 121, so that the pH of the sample to be injected can be more easily confirmed.

< Experimental Example  5> pH measurement using paper pH sensor

In this experimental example, the pH of the sample was measured using the paper pH sensor 100 prepared in Example 4

More specifically, a buffer solution sample having a pH of 13 was injected into the fluid injection port 131 of the paper pH sensor 100 produced in Example 3, and the difference in color change occurring in each detection means was observed, The results are shown in Fig. As a reference, the buffer solution having the pH 13 described above was used as the buffer solution of the corresponding pH shown in Table 2 in Example 1-1. That is, a buffer solution of KCl + NaOH having a pH of 13 was used as the buffer solution used in the experimental example of the present invention.

Referring to FIG. 19, the pH indicator may be selected from the group consisting of crystal violet, ethyl violet, quinaldine Red, tetrabromophenol blue, bromocresol green, A mixed solution of Chlorophenol Red, bromothymol blue, phenol red, phenolphthalein, Thymolphthalein, phenolphthalein and titanium yellow, The color of the detection region 121 impregnated with tropaeolin O and titanium yellow was changed.

In particular, the pH paper sensor of FIG. 19 displays the scale and the pH of the indicator impregnated in the detection region 121 at a portion adjacent to the detection region 121, so that the pH of the sample to be injected can be more easily confirmed.

< Experimental Example  6> pH measurement using paper pH sensor

In this Experimental Example, the pH of the sample was measured using the paper pH sensor manufactured in Example 5

More specifically, a buffer solution sample having a pH of 13 was injected into the fluid injection port of the paper pH sensor prepared in Example 5, and the difference in color change occurring in each detection means was observed. The results are shown in FIG. 20 . As a reference, the buffer solution having the pH 13 described above was used as the buffer solution of the corresponding pH shown in Table 2 in Example 1-1. That is, a buffer solution of KCl + NaOH having a pH of 13 was used as the buffer solution used in the experimental example of the present invention.

Referring to FIG. 20, a crystal violet (TCI), ethyl violet (TCI), quinaldine Red (TCI), tetrabromophenol blue (Sigma Aldrich), bromocresol (Bromocresol Green), bromocresol purple (Sigma Aldrich), cetyltriammonium bromide (Sigma Aldrich), bromothymol blue (Samseon Pure Chemical Industries) Thymol blue, thymolphthalein (Sigma Aldrich), thymol blue and titanium yellow (TCI), and a mixture of thymol blue and thiamolium bromide. , The color of the detection area impregnated with the tropaeolin O (TCI), Titan yellow (TCI) pH indicator changed.

In particular, the pH paper sensor of FIG. 20 can display the pH of the indicator immersed in the scale and the detection area in the vicinity of the detection area to more easily confirm the pH of the sample to be injected.

<Experimental Example 7>

Experimental Example  7-1. PH measurement using paper pH sensor

On the other hand, the equilibrium of the pH detection reaction of HIn -> H ⁺ + In ^- can be shifted by adding a cationic substance and a nonionic surfactant to the pH indicator reagent solution used for the pH sensor.

When the cationic substance is added to the pH indicator reagent solution, the equilibrium of the pH detection reaction shifts to the right. As a result, the pH indicator reagent before adding the cationic surfactant changes color at a pH lower than the pH value .

On the other hand, when the nonionic surfactant is added to the pH indicator reagent solution, the equilibrium of the pH detection reaction shifts to the left, and as a result, the color change occurs at a pH higher than the pH value at which the existing pH indicator reagent shows color change in the pH sensor.

In this experiment, a solution of bromothymol blue, which is one of the pH indicating reagents used in the paper pH sensor, was used.

On the other hand, the bromothymol blue is an acid base indicator, and the color change range is pH 6.0 to 7.6. The acid color is yellow, the basic color is blue, and the alkaline solution is light dichroism.

21 is a graph showing the result of measuring the pH of a sample using the paper pH sensor using the colorimetric method prepared in Example 1 of the present invention.

FIG. 21 shows the color change according to the pH change of the bromothymol blue solution according to the pH value of the surface of the paper coated with the indicator solution and the RGB value change graph of the color changed at the color change .

Experimental Example  7-2. PH measurement using paper pH sensor

When cetyltri ammonium bromide, which is a cationic substance, is added to the bromothymol blue solution, the color change occurs at a pH value lower than the pH at which the existing bromothymol blue solution causes color change. The color change according to the pH value of the paper surface and the RGB value change graph of the color changing at the color change are shown.

Experimental Example  7-3. PH measurement using paper pH sensor

When the nonionic surfactant Triton X-100 is added to the bromothymol blue solution, the color change occurs at a pH value higher than the pH at which the conventional bromothymol blue solution causes color change. The color change according to the pH value of the surface and the RGB value change color changing at the color change are shown in the graph.

While the present invention has been particularly shown and described with reference to the particular embodiments thereof, those skilled in the art will appreciate that such specific embodiments are merely exemplary and that the scope of the invention is not limited thereby. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

100: Paper pH sensor
110: paper layer
111: hydrophobic member 112: fluid passage
113: Porous member 114: hollow
115: hollow-paper passage
120:
121: detection area
1211: first detection area 1212: second detection area
1213: third detection area 1214: fourth detection area
1215: fifth detection area 1216: sixth detection area
122: marking means 123:
124:
130: upper hydrophilic film
131: inlet 132: outlet
140: Lower hydrophilic film
150: adhesive layer
160: intermediate adhesive layer 161: space
170: Lower adhesive layer
180: upper adhesive layer 190: middle layer

Claims (23)

A paper layer including a fluid passage made of a hollow-paper passage, wherein a detection portion in which a plurality of detection regions are disposed along the longitudinal direction of the fluid passage is formed;
An upper hydrophilic film disposed on the upper surface of the paper layer and having a fluid inlet and an outlet; And
A lower hydrophilic film disposed on a lower surface of the paper layer; / RTI &gt;
Characterized in that the paper layer is made of a hydrophobic member except for the inner surface of the fluid passage and the detection portion and the plurality of detection regions are impregnated with respective reagents which induce a color change at pH 0 to pH 14. [ Paper pH sensor used.
The method according to claim 1,
The upper and lower hydrophilic films
Wherein the paper layer is adhered to upper and lower surfaces of the paper layer by an adhesive layer, respectively.
The method according to claim 1,
The upper hydrophilic film is in close contact with the intermediate adhesive layer,
Wherein the intermediate adhesive layer has a space formed therein to accommodate the paper layer and the lower hydrophilic film.
The method of claim 3,
Wherein the lower hydrophilic film further comprises a lower adhesive layer on the lower surface.
The method according to claim 1,
Wherein the upper hydrophilic film and the lower hydrophilic film are transparent substrates.
The method according to claim 1,
The upper hydrophilic film or paper layer
And a marking means is formed in a region corresponding to the detection region.
A paper layer including a fluid passage made of a hollow-paper passage, and having a detection portion in which a plurality of detection regions are disposed along a longitudinal direction of the fluid passage;
An upper adhesive layer disposed on the upper surface of the paper layer and having a fluid inlet and an outlet;
A lower hydrophilic film disposed on a lower surface of the paper layer; And
A lower adhesive layer disposed on the lower surface of the lower hydrophilic film; / RTI &gt;
Characterized in that the paper layer is made of a hydrophobic member except for the inner surface of the fluid passage and the detection portion and the plurality of detection regions are impregnated with respective reagents which induce a color change at pH 0 to pH 14. [ Paper pH sensor used.
8. The method of claim 7,
Wherein the paper layer and the lower hydrophilic film are in close contact with each other by the upper adhesive layer and the lower adhesive layer,
And an intermediate layer formed between the upper adhesive layer and the lower adhesive layer and having a space therein to receive the paper layer and the lower hydrophilic film so that the paper layer and the upper adhesive layer and the lower hydrophilic film and the lower adhesive layer are uniformly adhered Wherein the pH sensor is a pH sensor using a colorimetric method.
8. The method of claim 7,
The upper and lower adhesive layers
Characterized in that an adhesive material is applied on one surface of the paper.
8. The method of claim 7,
The paper layer or top adhesive layer
And a marking means is formed in a region corresponding to the detection region.
8. The method of claim 1 or 7,
The reagent comprises a pH indicator,
The pH indicator may be selected from the group consisting of malachite, brilliant green, methyl green, methyl violet, crystal violet, eosin bluish, ethyl violet, m-cresol purple, thymol blue, p-xylenol blue, 2,2 ', 2', 4,4'-pentamethoxytriphenyl Triphenylcarbinol, quinaldine red, 2,4-dinitrophenol, methyl yellow, and the like. , Bromochlorophenol, bromophenol blue, tetrabromophenol blue, congo red, methyl orange, bromocresol green, 2,5-dinitrophenol, methyl red, chlorophenol red, bromocresol purple, bromophenol, Bromophenol red, nitrazine yellow, bromoxylenol blue, bromothymol blue (BTB), neutral red, phenol red, 3-nitrophenol (3) natrophenol, 1-naphtholphthalein, phenolphthalein, thymolphthalein, alizarin yellow GG, tropaeolin O, indigo carmine (indigo wherein the pH sensor is at least one selected from the group consisting of carmine, epsilon blue, alkali blue and titanium yellow.
12. The method of claim 11,
The reagent
At least one said pH indicator; And
And an auxiliary compound having a high interaction with the pH indicator,
Wherein the indicator aqueous solution is capable of changing the acid dissociation constant (pKa) of the pH indicator.
13. The method of claim 12,
The auxiliary compound consists of a pH indicator and an organic compound that causes ion-ion or ion-dipole interaction,
The auxiliary compound may be selected from the group consisting of cetyltrimethyl ammonium sulfate, dodecyl pyridinium bromide, sodium dodecyl sulfate, sodium dodecyl sulfonate, sodium hexadecano Sodium hexadecanoate, 4- (1,1,3,3-tetramethylbutyl) phenyl-polyethylene glycol (4- (1,1,3,3-Tetramethylbutyl) phenyl-polyethylene glycol, dodecylpenta but are not limited to, dodecyl penta (ethylene oxide), trihexyl- (tetradecyl) phosphonium chloride, trimethylpyrazolium methylsulfate, At least one selected from the group consisting of 1-butyl-3-methylimidazolium hydrogen sulfate and 1-ethyl-3-methylimidazolium chloride Paper pH sensor using a colorimetric method, characterized in that.
(a) fabricating a paper layer comprising a hollow-paper path;
(b) preparing an upper hydrophilic film and a lower hydrophilic film; And
(c) disposing the upper hydrophilic film and the lower hydrophilic film on the upper and lower surfaces of the paper layer, and fixing the upper and lower hydrophilic films using a pressing process of adhesion or adhesion; Wherein the pH of the paper is measured by a colorimetric method.
(a) fabricating a paper layer comprising a hollow-paper path;
(b) fabricating an upper adhesive layer and a lower adhesive layer; And
(c) disposing the upper adhesive layer and the lower adhesive layer on the upper and lower surfaces of the paper layer, and fixing the upper and lower adhesive layers using a pressing process of the adhesive,
The step (c) includes the steps of inserting a paper layer and a lower hydrophilic film into the intermediate layer 190 having a space formed therein, and fixing the lower adhesive layer to the lower part of the lower hydrophilic film using a pressing process; Wherein the pH of the paper is measured by a colorimetric method.
16. The method according to claim 14 or 15,
The step (a)
Forming a fluid passage and an outer wall of the detection region in the paper layer in a hydrophobic manner;
Forming a hollow-paper passage by using a CO ₂ cutter at a portion spaced apart from the outer wall by a predetermined distance to the inside of the fluid passage; And
Impregnating the detection area with a reagent; Wherein the pH of the paper is measured by a colorimetric method.
17. The method of claim 16,
The step of forming the outer wall of the fluid passage and the detection area hydrophobic in the paper layer
At least one selected from the group of photolithography, ink-jet, wax printing, impregnation & hardening, imprinting and screen printing. Wherein the color filter is formed by using a colorimetric method.
15. The method of claim 14,
The step (b)
And forming a fluid injection port and a detection region by cutting the upper hydrophilic film to form a paper hydrophilic film.
16. The method of claim 15,
The step (b)
And cutting the upper adhesive layer to form a fluid inlet and an outlet. &Lt; RTI ID = 0.0 &gt; 8. &lt; / RTI &gt;
15. The method of claim 14,
The step (c)
Attaching the paper layer between the upper hydrophilic film and the lower hydrophilic film using an adhesive layer; And
Pressing the upper hydrophilic film, the lower hydrophilic film and the paper layer attached by the adhesive layer; Wherein the pH of the paper is measured by a colorimetric method.
21. The method of claim 20,
The step of pressing the upper hydrophilic film, the lower hydrophilic film and the paper layer
And thermo-compression bonding is carried out at a temperature of 45 to 95 ° C.
15. The method of claim 14,
The step (c)
Inserting a paper layer and a lower hydrophilic film into an intermediate adhesive layer having a space therein; And
Adhering the lower adhesive layer to the lower portion of the lower hydrophilic film; Wherein the pH of the paper is measured by a colorimetric method.
16. The method of claim 15,
The upper and lower adhesive layers
A lamination film,
And thermocompression bonding is performed at 70 to 130 ° C.

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