KR101707987B1 - Automatic quantitative dispensing device, continuous type gene extraction-amplification apparatus including the device and operating method of the apparatus - Google Patents

Automatic quantitative dispensing device, continuous type gene extraction-amplification apparatus including the device and operating method of the apparatus Download PDF

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Publication number
KR101707987B1
KR101707987B1 KR1020150084880A KR20150084880A KR101707987B1 KR 101707987 B1 KR101707987 B1 KR 101707987B1 KR 1020150084880 A KR1020150084880 A KR 1020150084880A KR 20150084880 A KR20150084880 A KR 20150084880A KR 101707987 B1 KR101707987 B1 KR 101707987B1
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South Korea
Prior art keywords
gene
sample
bar magnet
amplification
extraction
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KR1020150084880A
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Korean (ko)
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KR20160148201A (en
Inventor
유문조
송강희
신은심
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휴스텝스 주식회사
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1002—Reagent dispensers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346—Heating or cooling arrangements
    • G01N2035/00356—Holding samples at elevated temperature (incubation)
    • G01N2035/00366—Several different temperatures used
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/103—General features of the devices using disposable tips
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid
    • G01N2035/1039—Micropipettes, e.g. microcapillary tubes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid
    • G01N2035/1041—Ink-jet like dispensers

Abstract

The automatic dosing dispenser according to the present invention is characterized in that the automatic dosing dispenser according to the present invention includes a volumetrically variable portion in which a connecting end to which a bar magnet is engageable is formed at one end portion thereof and a volumetrically variable portion in which the other end A pipette section connected to the pipette section, and a latching protrusion formed on an outer circumferential surface of the pipette section.
In addition, the continuous gene extraction-amplification apparatus according to the present invention comprises a sample kit mounting part for mounting a sample kit capable of performing gene extraction through an automated series of steps, a PCR amplifier for performing gene amplification, A scavenging portion disposed between the seating portion and the PCR amplifier and a bar magnet moving rail for transferring the bar magnet from the sample kit seating portion toward the PCR amplifier in accordance with a given processing recipe, A magnetic bead storage unit in which a plurality of magnetic beads are stored, a sample sample storage unit capable of injecting a liquid sample, a wash buffer storage unit, a solution buffer storage unit, And the automatic disc dispenser having a built-in automatic dosing dispenser is disposed at an interval. The notch, which enables the removal of separated contamination prevention tips sandwiched portion consists of a structure formed on a side.

Description

[0001] The present invention relates to an automatic quantitative dispensing device, a continuous quantitative dispensing device, and a continuous type gene extraction-amplification device including the automatic quantitative dispenser and the dispenser,

The present invention relates to a one-step automatic test (or diagnostic) apparatus capable of continuously extracting and amplifying a gene, more specifically, a method of inhaling a gene material extracted in a gene extraction step, An automatic quantitative dispenser configured to transfer (distribute) a predetermined amount of genetic material into the dispensing container by being transferred to an amplification stage, and a continuous gene extraction-amplifying apparatus including the automatic quantitative dispenser, And extracting and amplifying the same.

As molecular biology and genetic engineering develop, genetic tests targeting animals and humans are introduced and applied.

Examples of the substance to be subjected to genetic testing include messenger RNA encoding DNA or protein, and proteins and specific metabolites associated therewith.

In addition, for the purpose of genetic testing, the prediction of the risk of genetic disease and the identification of the donor are included.

Genetic testing requires gene extraction and gene amplification, among which gene extraction begins at the sample collection stage.

As an example, in the sample collection step, a cotton swab is rubbed on the skin of a chicken, a specimen containing a gene component of a contaminant such as bacteria is put on a swab and the swab is put into a test tube containing a specific solution. In this way, various foreign substances are dispersed in a specific solution together with specimens on the swab. In this process, the protein or phospholipid surrounding the cell nucleus is destroyed by the specific solution, and the gene in the nucleus is released into a specific solution .

Next, the collection step of collecting the gene dispersed in the specific solution in the test tube using magnetic beads is carried out.

The magnetic beads used in the collecting step are well known in the art and include a ball-shaped magnetic material (for example, ferrous metal), a coating layer of plastic or glass surrounding the magnetic material, and a silica coating layer This silica coating layer has a property of attracting a gene and adsorbing it.

Therefore, when a large number of magnetic beads are put into a test tube, the gene dispersed in a specific solution sticks to the surface of the magnetic bead (that is, the silica coating layer).

In this state, when the rod magnet (more specifically, the electromagnet) is put into the test tube, the magnetic body in the magnetic bead is attracted by the magnetic force of the rod magnet, so that the magnetic bead collects around the rod magnet.

Then, a washing step for removing impurities (foreign substances) other than the gene adsorbed to the magnetic beads is carried out.

The washing step is usually carried out twice, but there is no limitation on the number of times of washing. Further, the washing step is carried out in a container (for example, a test tube) containing a washing buffer (washing liquid or washing liquid).

More specifically, in the first cleaning step, a bar magnet to which a plurality of magnetic beads are adhered is transferred from the previous collection step to the cleaning step, and then the bar magnet is inserted into a test tube containing a washing buffer, Thereby removing the magnetic force of the electromagnet.

This means that a number of magnetic beads that stick to the bar magnet will fall off the bar magnet and disperse into the wash buffer in the test tube.

Thereafter, a current is supplied to the rod magnet from which the magnetic force is removed to re-magnetize the rod magnet, so that a plurality of magnetic beads to which the gene (dielectric material) is adsorbed are stuck to the rod magnet again.

Subsequently, the rod magnet having a plurality of magnetic beads stuck thereto is transferred to the second washing step, the magnetic force of the rod magnet is removed as described in the first washing step, and the magnetic force of the rod magnet is again The second washing is carried out.

Next, a gene elution step is carried out.

This gene elution step is carried out using a solution buffer (eluent) which prevents the gene from sticking to the magnetic beads.

More specifically, the rod magnet that has been transferred to the gene elution step in the previous washing step is inserted into a test tube containing a solution buffer and the current is blocked so that the magnetic beads attached to the rod magnet are dispersed in the solution buffer.

After a certain period of time has elapsed in this state, if the current is again flowed through the rod magnet, only the magnetic bead that has fallen off the gene sticks to the rod magnet.

For example, Patent Document 2 (gene extraction method by one step) and Patent Document 4 (automatic purification apparatus, multi-well plate kit and method of extracting nucleic acid from biological sample) ) Are known.

The eluate containing only the gene through each of the above steps is quantitated using a pipette or the like, and then gene amplification (amplification) is performed by injecting into a PCR (Polymerase Chain Reaction) amplifier capable of temperature control.

Various gene amplification methods are known from Patent Document 1 (gene amplification apparatus and its manufacturing method) and Patent Document 3 (non-contact heating gene amplification system).

However, existing gene testing equipment is not composed of a gene (nucleic acid) extracting device and a gene amplifying device, so that the process from gene extraction to gene amplification can not be automated.

This is because there is no quantitative frequency divider capable of automatically supplying an eluting solution containing a gene extracted by a gene extracting apparatus (a genetic buffer) to a gene amplifying apparatus.

Patent Document 1: Publication No. 10-2015-0024050 Patent Document 2: Publication No. 2002-0029477 Patent Document 3: Registration No. 10-1302748 Patent Document 4: Registration No. 10-1025135

Disclosure of the Invention The present invention has been conceived to solve the problems of such conventional gene inspection equipment, and it is an object of the present invention to provide an automatic quantitative dispenser capable of transplanting a certain amount of a nucleic acid- There is a purpose in doing.

It is another object of the present invention to provide a continuous gene extraction-amplification apparatus including such an automatic quantitative dispenser. Particularly, since the continuous gene extraction-amplification apparatus according to the preferred embodiment of the present invention employs permanent magnets as components for attracting magnetic beads used for gene collection, as compared with the case where electromagnets are used, And it is an object of the present invention to provide a device that is compact and portable.

It is another object of the present invention to provide a method in which extraction and amplification of a gene can be carried out continuously through a series of processes.

In order to achieve the above object, the present invention has the following features.

[1] A liquid ejecting apparatus comprising: a volume varying section formed at one end of a connecting end to which an external actuator can be coupled; and a volume varying section for varying the volume of the volume varying section generated when the external actuator is coupled to the connecting end of the volume varying section A pipette portion connected to the other end of the volume variable portion so as to be sucked or discharged; and a latching jaw formed on an outer circumferential surface of the pipette portion.

[2] A kit for a sample kit in which a sample kit capable of performing gene extraction is settled through an automated series of steps, a PCR amplifier for performing gene amplification, a waste part disposed between the sample kit mounting part and the PCR amplifier, And a bar magnet transfer rail for transferring the bar magnet from the sample kit seating portion to the PCR amplifier in accordance with a given processing recipe, wherein the sample kit comprises: A contamination prevention tip storage portion in which a contamination prevention tip is stored; A magnetic bead storage part for storing a plurality of magnetic beads having a magnetic substance capable of sticking to the rod magnet and a silica coating layer on which the gene can be adsorbed; A sample sample storage unit capable of injecting a liquid sample in which a gene and unavoidable impurities are mixed; A wash buffer reservoir that relatively increases the concentration of the gene and relatively lowers the impurity concentration; A solution buffer storing a solution buffer for containing only a gene; And the automatic dosing dispenser with the automatic dosing dispenser according to the above [1] are disposed at an interval from each other. Further, the waste part is constituted by the entrance of the stick magnet and the contamination prevention tip Wherein a notch is provided on one side to enable separation (removal) of the gene extraction / amplification device.

[3] The continuous gene extraction-amplification apparatus according to the above [2], wherein two or more wash buffer storage units are arranged adjacent to each other with an interval therebetween.

[4] A continuous gene extraction-amplification method characterized by automating all processes from gene extraction to gene amplification using the continuous gene extraction-amplification apparatus described in [2] or [3] above.

According to the present invention, extraction and amplification of genes can be automatically performed through a series of sequential processes by using an automatic quantitative dispenser of simple configuration.

In order to overcome the difficulty of carrying by the components such as the coil when the conventional electromagnet is used, the size of the permanent magnet is reduced to a portable size so that a series of extraction and amplification The process can be performed efficiently.

FIG. 1 is a time chart showing a schematic configuration and an operating state of an automatic dosing dispenser according to an embodiment of the present invention. FIG. 1 (a) is a view showing a state in which an automatic quantitative dispenser (B) shows the compression state in which compression by the external actuator is performed in the state of (a), (c) in the state of (b), the external actuator moves in the opposite direction (D) shows that the automatic dosing dispenser, which sucks a certain amount of liquid, is separated from the test tube and can be transferred to a subsequent step or a subsequent step Indicates a departure state.
BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to an automatic quantitative dispensing apparatus,
3 is a plan view showing an example of the sample kit in Fig.
FIG. 4 is a diagram for explaining a process of successively extracting and amplifying genes using the continuous gene extraction-amplification apparatus shown in FIG. 2; FIG.
FIG. 5 is a diagram for explaining a series of processes for successively extracting and amplifying genes using the continuous gene extraction-amplification apparatus shown in FIG. 2, corresponding to steps 3 to 6 of FIG.

In order to fully understand the present invention, a preferred embodiment of the present invention will be specifically described below based on the accompanying drawings.

Here, the embodiments of the present invention can be modified into various forms, and thus the scope of the present invention should not be interpreted as being limited to the embodiments described below. In other words, the embodiments described below are provided only for those of ordinary skill in the art to more fully understand the present invention.

Further, in each drawing, the shapes of main components and the like may be exaggerated in order to better understand the description of the embodiments.

Further, detailed descriptions of well-known functions and configurations, which are considered to be unnecessarily disturbing the technical gist of the present invention, are omitted.

[Configuration of Automatic Quantitative Dissipation Cycle]

1 shows a schematic configuration and an operating state of the automatic dosing dispenser 100 according to an embodiment of the present invention in a time sequence. FIG. 1 (a) shows a state in which the automatic dosing dispenser 100 is placed in a test tube 300 (B) shows a compression state in which compression by the external actuator is performed in the state of (a). Fig. In the state (b) of FIG. 3 (c), when the external actuator operates in a direction opposite to the previous state, the liquid 400 in the test tube 300 is sucked into the pipette portion 120 of the automatic dosing dispenser 100 (D) shows a state in which the automatic dosing dispenser 100 in which a certain amount of the liquid 400 is sucked is separated from the test tube 300 and is capable of being transferred to a subsequent step or a subsequent step.

1, the automatic dosing dispenser 100 according to one embodiment of the present invention includes a volume variable portion 110 having a connection end 111 to which an external actuator can be coupled, (110) so as to be able to suck or discharge a predetermined amount of liquid in response to a volume change amount of the volume varying portion (110) which is generated when the liquid is coupled to the connection end (111) of the volume varying portion A pipette part 120 connected to the other end of the pipette part 120 and an engagement protrusion 130 formed on an outer circumferential surface of the pipette part 120.

When the external actuator moves toward the pipette part 120, the volume (internal volume) decreases. When the external actuator moves toward the pipette part 120, the volume of the volume variable part 110 increases and the volume of the pipette part 120 Or a predetermined amount of the liquid is discharged from the pipette portion 120. The pipette portion 120 is provided with a plurality of liquids.

The engaging step 130 formed on the outer circumferential surface of the pipette part 120 is preferably an annular ring, but a part of the ring may be cut.

The automatic dosing dispenser 100 can be integrally manufactured by, for example, plastic injection molding.

The automatic quantitative dispenser 100 according to the embodiment of the present invention is developed for automatically transferring a certain amount of a solution buffer containing a gene from a gene extracting apparatus to a gene amplifying apparatus, It may be used for the quantitative transfer of another substance, for example, a liquid chemical substance.

In other words, the automatic dosing dispenser 100 according to the present invention can be used wherever the quantitative transfer of the liquid material is required.

In order to emphasize that there is no limitation in the use range of the automatic dosing dispenser 100 according to the present invention, the automatic dosing dispenser 100 according to the present invention has an external actuator (not shown) for the driving element causing the volume change of the volume varying section 110, , And this external actuator corresponds to the bar magnet 200 when it is limited to the genetic testing equipment field.

The bar magnet 200 is preferably composed of a permanent magnet.

[Continuous gene extraction - Construction of amplification equipment]

FIG. 2 is a block diagram showing a schematic configuration of a continuous gene extraction-amplification apparatus according to an embodiment of the present invention, and FIG. 3 is a plan view showing an example of the sample kit in FIG.

2 and 3, the continuous gene extraction-amplification apparatus according to an embodiment of the present invention includes a sample kit A1 capable of performing gene extraction through an automated series of processes, A PCR amplifying device D for performing gene amplification; a waste part C disposed between the sample kit mounting part A and the PCR amplifying device D; and a given processing recipe And a bar magnet moving rail (B) for transferring the bar magnet (200) from the sample kit mounting portion (A) to the PCR amplifier (D)

More specifically, the bar magnet moving rail (B) is provided above the space between the sample kit mounting portion (A) and the PCR amplifier (D).

2, the bar magnet 200 is movable in the horizontal direction along the bar magnet moving rail B, and the bar magnet 200 is moved in the horizontal direction along the bar magnet moving rail B as well as the sample kit seating portion A, the waste portion C, D in the vertical direction.

As shown in Fig. 3, the sample kit A1 has a structure of 7 rows x 10 rows, and the number of rows may be different.

In the sample kit A1, a total of seven storage units are formed in one row at a distance from each other. More specifically, the sample kit A1 includes a contamination prevention tip 500, which stores a contamination prevention tip 500 for fitting the end of the bar magnet 200, a magnetic bead storage part a2 in which a plurality of magnetic beads having a silica coating layer capable of sticking to the rod magnet 200 and a gene can be adsorbed and a magnetic bead storage part a2 in which a gene and unavoidable impurities are mixed, A wash buffer storage unit (a4, a5) for relatively increasing the concentration of the gene and relatively lowering the impurity concentration, and a solution buffer for containing only the gene A stored aliquot buffer storage unit a6, and an automatic volume dividing unit a7 having the above-described automatic quantitative dispenser 100 incorporated therein.

4, a notch for enabling the entry of the bar magnet 200 and the separation and removal of the anti-fouling tip 500 fitted to the end of the bar magnet 200 is provided at one side As shown in Fig.

The PCR amplifier D includes an amplification section D1, a lower heater D2 fixed to the lower portion of the amplification section D1, and a lower heater D2 fixed on the amplification section D1, A movable upper heater D3, and an upper heater moving rail D4 to which the upper heater D3 is transportably installed. Since this configuration is well known in the art, a detailed description thereof will be omitted.

[Extracting and amplifying genes in series]

FIG. 4 is a diagram for explaining a process of successively extracting and amplifying genes using a continuous gene extraction-amplification apparatus according to the present invention, and FIG. 5 is a diagram corresponding to steps 3 to 6 of FIG.

First, the sample kit A1 illustrated in FIG. 3 is placed in the sample kit mounting portion A.

In this state, the bar magnet 200 is moved to just above the contamination prevention tip storage section a1 of the sample kit A1.

Next, the rod magnet 200 is lowered to insert the dirt-preventing tip 500 made of rubber contained in the dirt-preventing tip storage portion a1 into the end of the rod magnet 200 (step (1)).

When the end of the bar magnet 200 is covered with the anti-dirt tip 500, the bar magnet 200 is raised as it is, and then the bar magnet 200 is moved just above the magnetic bead storage part a2.

5, the magnetic bead 600 contained in the magnetic bead storage part a2 includes a ball-shaped magnetic body 610, a coating layer 620 made of plastic or glass surrounding the magnetic body 610, And the silica coating layer 630 surrounding the coating layer 620. When the end portion of the bar magnet 200 is introduced into the magnetic bead storage portion a2, a large number of the magnetic beads stored in the magnetic bead storage portion a2 The magnetic bead 600 sticks to the bar magnet 200 (step (2)).

On the other hand, in the sample sample storage part (a3), a predetermined amount of liquid (i.e., sample sample) in which the protein or phospholipid surrounding the cell nucleus is broken by the specific solution and the gene in the cell nucleus is loosened is injected in advance.

In this state, when the rod magnet 200 with the magnetic beads 600 attached thereto is introduced into the sample storage section a3, impurities are adsorbed to the silica coating layer 630 of the magnetic bead 600 together with the gene ③ step).

Then, after the bar magnet 200 is raised, the bar magnet 200 is moved to a position just above the wash buffer storage unit a4.

When the bar magnet 200 is positioned just above the wash buffer storage unit a4, the bar magnet 200 is lowered to lock the end of the bar magnet 200 with the magnetic bead 600 in the wash buffer (Shaking or stirring) in the magnetic bead 600, and the impurities attached to the magnetic bead 600 are separated to some extent (step 4).

Subsequently, the bar magnet 200 to which the magnetic beads 600 are attached is moved up to the second wash buffer storage unit a5, and the same process as in the step (4) is repeated (step (5)). .

The bar magnet 200 having the magnetic bead 600 adhered thereto is moved just above the solution buffer storage part a6 and then lowered so as to enter the solution buffer storage part a6, The magnetic bead 600 itself vibrates in the bar magnet 200 while the bar magnet 200 is attached to the bar magnet 200 in a state in which the bar magnet 200 is immersed in the illusion buffer so that the gene is separated from the surface of the magnetic bead 600, (⑥ step).

Then, after the bar magnet 200 is moved to the position where the waste portion C is located, the bar magnet is raised or lowered, or the waste portion C is raised or lowered, The tip 500 is peeled off.

In this state, the bar magnet 200 is moved to a position immediately above the automatic quantitative atomic number storage section a7 of the sample kit A1 and then lowered to move the end of the bar magnet 200 to the automatic quantitative dispenser 100 To the connection end 111 (step 8).

Thereafter, the rod magnet 200 coupled with the automatic dosing dispenser 100 is moved to the upper side of the constriction buffer storage section a6 and then lowered so that a predetermined amount Into the pipette part 120 of the automatic dosing dispenser 100. [

Thereafter, the rod magnet 200 coupled with the automatic dosing dispenser 100 is moved toward the PCR amplifier D while the upper heater D3 of the PCR amplifier D is moved to either side of the amplification unit D1 The pipette 120 is inserted into the amplification part D1 and the rod magnet 200 is moved to the volume variable part (not shown) so that the solution buffer containing the predetermined amount of gene is discharged from the pipette part 120 110).

In this way, everything from gene extraction to gene amplification is automatically processed.

100 ... Automatic dosing dispenser
110 ... Volume variable portion
111 ... Connection stage
120 ... Pipette portion
130 ... Jaw
200 ... Bar magnet (permanent magnet)
300 ... examiner
400 ... Liquid
500 ... Pollution prevention tips
600 ... Magnetic bead
610 ... Magnetic body
620 ... Coating layer
630 ... Silica coating layer
A ... The sample kit mounting portion
A1 ... Sample kit
a1 ... The pollution prevention tip storage portion
a2 ... Magnetic bead storage
a3 ... Sample sample storage section
a4 ... Wash buffer store
a5 ... Wash buffer store
a6 ... [0035]
a7 ... Automatic quantitative atomic frequency dispenser
B ... Bar magnet moving rail
C ... The waste part
D ... PCR amplifiers
D1 ... The amplifying unit
D2 ... Bottom heater
D3 ... Upper heater
D4 ... Upper heater moving rail

Claims (4)

A volume variable portion formed at one end of a connection end to which an external actuator can be coupled,
A pipette part connected to the other end of the volume variable part so as to suck or discharge a predetermined amount of liquid corresponding to a volume change amount of the volume variable part generated when the external actuator is driven in a state of being coupled to the connection end of the volume variable part;
And an engagement protrusion formed on an outer circumferential surface of the pipette section,
Wherein when the external actuator is lowered to the test tube containing the liquid, the latching jaw formed on the outer surface of the pipette portion is caught by the edge of the test tube and the liquid is sucked up when the volume of the volume varying portion is reduced and the external actuator is lifted. Quantitative dispenser. A sample kit mounting part for mounting a sample kit capable of performing gene extraction through an automated series of processes, a PCR amplifier for performing gene amplification, a waste part disposed between the sample kit mounting part and the PCR amplifier, A continuous gene extraction-amplification device comprising a bar magnet transfer rail for transferring a bar magnet from a sample kit mounting part to a PCR amplifier according to a recipe,
The sample kit includes:
A contamination prevention tip storage portion in which a contamination prevention tip for fitting the end portion of the bar magnet is stored;
A magnetic bead storage part for storing a plurality of magnetic beads having a magnetic substance capable of sticking to the rod magnet and a silica coating layer on which the gene can be adsorbed;
A sample sample storage unit capable of injecting a liquid sample in which a gene and unavoidable impurities are mixed;
A wash buffer reservoir that relatively increases the concentration of the gene and relatively lowers the impurity concentration;
A solution buffer storing a solution buffer for containing only a gene; And
Wherein the automatic dosing dispenser with the automatic dosing dispenser according to claim 1 is disposed at an interval from each other,
The discarding unit
Wherein a notch is formed at one side to enable the entrance of the bar magnet and the removal of the contamination preventing tip fitted to the end of the bar magnet. The method of claim 2,
Wherein at least two wash buffer storage units are disposed adjacent to each other with an interval therebetween. A continuous gene extraction-amplification method characterized in that all the steps from gene extraction to gene amplification are automated using the continuous gene extraction-amplification apparatus described in claim 2 or 3.
KR1020150084880A 2015-06-16 2015-06-16 Automatic quantitative dispensing device, continuous type gene extraction-amplification apparatus including the device and operating method of the apparatus KR101707987B1 (en)

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