KR101675258B1 - Multi luminescence spectroscopic method using multi band dichroic mirror and luminescence spectrophotometer using the same - Google Patents
Multi luminescence spectroscopic method using multi band dichroic mirror and luminescence spectrophotometer using the same Download PDFInfo
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- KR101675258B1 KR101675258B1 KR1020130018027A KR20130018027A KR101675258B1 KR 101675258 B1 KR101675258 B1 KR 101675258B1 KR 1020130018027 A KR1020130018027 A KR 1020130018027A KR 20130018027 A KR20130018027 A KR 20130018027A KR 101675258 B1 KR101675258 B1 KR 101675258B1
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- 238000004611 spectroscopical analysis Methods 0.000 title description 18
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/10—Arrangements of light sources specially adapted for spectrometry or colorimetry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/42—Absorption spectrometry; Double beam spectrometry; Flicker spectrometry; Reflection spectrometry
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- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
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- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/12—Generating the spectrum; Monochromators
- G01J2003/1213—Filters in general, e.g. dichroic, band
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The present invention relates to a multi-emission spectral analysis method using a multi-band type color selection mirror and a multi-emission spectral analysis apparatus using the same. The transmission type emissive spectrometer according to the present invention can use monochromatic light in various areas required for analyzing samples by using a multi-wavelength monochromatic light source and a multi-wavelength color selective mirror without mechanical replacement of the monochromator, In addition to being extremely easy to analyze, the structure is very simple and the production process is simple. In addition, since the monochromatic light reaching the target and the luminescence emitted from the excited target are selected by passing through the color selection mirror before and after the irradiated light reaches the target, Biotechnology and genetic engineering, such as DNA sequencing, the environmental field such as the analysis of organic or inorganic content in water, the analytical chemistry such as chemical reaction and quantum efficiency calculation, and the detection of luminescent substances in food But it can be usefully used throughout industry such as the quantification of polymer compounds, film coating field, and the like.
Description
The present invention relates to a multi-emission spectral analysis method using a multi-band type color selection mirror and a multi-emission spectral analysis apparatus using the same.
Fluorescence refers to light generated when light is irradiated on a sample, when the energy state inside the sample transitions from a ground state to an excited state and returns to the ground state within a few nanoseconds within a short time. Phosphorescence is similar to fluorescence but when it returns to the ground state, it emits light in a delayed time rather than fluorescence because it passes through the triplet state, which is an intermediate energy level.
The emission spectrometer is a device used for analyzing the optical and photochemical properties of a sample by measuring fluorescence or phosphorescence generated from a sample irradiated with light by wavelength and clarifying the microstructure of the material. Such an emission spectrometer can be applied to various fields such as biotechnology and genetic engineering such as DNA structure analysis and DNA sequencing, environmental field such as analysis of the content of organic matters in water, analytical chemistry such as calculation of chemical reaction and quantum efficiency, Etc., as well as in the field of polymeric compound quantification, film coating, and the like.
1 is a schematic view schematically showing a configuration of a conventional general emission spectrometer.
1, in a conventional emission spectrometer, monochromatic light coinciding with an absorption wavelength of a phosphor attached to a
However, since the conventional emission spectroscopic analyzer has a structure for obtaining a single light emission image according to the light irradiated to the
In order to solve such a problem, a multi-emission spectrometer has been developed which includes a plurality of light sources so as to irradiate various light to the
That is, since separate electronic and mechanical equipments are required to replace the monochromator, the filter, and the single-wavelength color discriminating mirror included in the light source unit of the conventional multi-emission spectrometer, Mechanical vibrations are generated to cause malfunction of the device, and a separate time is consumed to replace the light source unit and the filter, so that a desired luminescent image can not be obtained quickly and easily. Therefore, it is required to develop a multi-emission spectrometer capable of easily and rapidly obtaining a large number of light emission images even without mechanical movement.
Researches for multi-emission spectroscopic analysis have been actively conducted so far and include a plurality of image acquisition units corresponding to the number of irradiated light sources to thereby easily and rapidly analyze a plurality of light emission images without mechanical movement. (Patent Document 1). However, since the light emission image observing apparatus includes a plurality of image acquisition units in a limited space, the structure is complicated, requiring a high degree of proficiency in the production process, and there is a problem that the optical arrangement can be easily turned on even a small impact.
Thus, the present inventors confirmed that a multi-emission spectrometer using a multi-wavelength monochromatic light source and a multi-wavelength color selective mirror can measure a plurality of emission spectral data at various monochromatic wavelengths without mechanical movement for replacing the light source and the filter Thus completing the present invention.
An object of the present invention is to provide a transmission type emission spectroscopic analysis method.
It is another object of the present invention to provide a transmission type emission spectroscopy apparatus using a transmission type emission spectroscopic analysis method.
It is still another object of the present invention to provide a reflection type emission spectroscopic analysis method.
It is another object of the present invention to provide a reflection type emission spectroscopy apparatus using a reflection type emission spectroscopic analysis method.
In order to achieve the above object,
The present invention relates to a method of manufacturing a multi-wavelength monochromatic light source, comprising the steps of: (1) reflecting monochromatic light generated by a multi-wavelength monochromatic light source through a first color selection mirror;
The step of reaching the monochromatic light reflected in the step 1 to excite the target and obtain the transmissive light emission from the excited target (step 2);
Removing the monochromatic light other than the luminescent light by using the second color discriminating mirror and separating only the luminescent light (step 3); And
And a step of detecting and analyzing the light emitted by the detector in the step (3) by a detector (step 4).
The present invention also provides a light source device comprising: a light source section for generating monochromatic light;
A first color discriminating mirror for reflecting the monochromatic light generated from the light source unit;
A sample portion including the target, wherein the monochromatic light reflected from the first color selecting mirror reaches the target to excite the target and the transmissive light emitted from the excited target emits light;
A second color discriminating mirror for removing monochromatic light other than the luminescent light from the mixed light of the light emitted from the sample and the monochromatic light other than the luminescent light and separating only the luminescent light; And
And a detector for detecting and analyzing the light emitted by the second color-separating mirror.
Further, the present invention provides a method of manufacturing a color filter, comprising the steps of: (1) reflecting monochromatic light generated by a multi-wavelength monochromatic light source through a color selection mirror;
A step (step 2) of obtaining the reflective light emission emitted from the excited target by causing the monochromatic light reflected at the step 1 to reach the target to excite the target;
Removing the monochromatic light other than the luminescent light by using the color selection mirror of step 1 and separating only the luminescent light (step 3); And
And a step of detecting and analyzing the luminescence separated in the step 3 by a detector (step 4).
The present invention also provides a light source device comprising: a light source section for generating monochromatic light;
A color discriminating mirror for simultaneously performing a function of reflecting the monochromatic light generated from the light source unit and a function of selecting only reflection type light emitted from the sample unit;
A sample portion including the target, wherein the monochromatic light reflected from the color selection mirror reaches a target to excite a target and emit a transmissive light emission from the excited target; And
And a detection unit for detecting and analyzing the light emitted by the color selection mirror.
The transmission type emissive spectrometer according to the present invention can use monochromatic light in various areas required for analyzing samples by using a multi-wavelength monochromatic light source and a multi-wavelength color selective mirror without mechanical replacement of the monochromator, In addition to being extremely easy to analyze, the structure is very simple and the production process is simple. In addition, since the monochromatic light reaching the target and the luminescence emitted from the excited target are selected by passing through the color selection mirror before and after the irradiated light reaches the target, Biotechnology and genetic engineering, such as DNA sequencing, the environmental field such as the analysis of organic or inorganic content in water, the analytical chemistry such as chemical reaction and quantum efficiency calculation, and the detection of luminescent substances in food But it can be usefully used throughout industry such as the quantification of polymer compounds, film coating field, and the like.
1 is a schematic diagram schematically showing a configuration of a conventional general emission spectrometer.
2 is a schematic diagram schematically showing a configuration of a transmission type emission spectrometer according to the present invention.
FIG. 3 is a graph showing transmission and reflection wavelengths of a general multi-band type color-separating mirror when a multi-wavelength light is irradiated.
FIG. 4 is a graph showing a wavelength of light passing through each section of a transmission type emission spectroscopic analyzer in a non-sample state according to Example 1. FIG.
FIG. 5 is a graph in which a wavelength graph of light passing through each section of a transmission type emission spectroscopic analyzer in a non-sample state according to the first embodiment is overlapped and compared.
FIG. 6 is a graph illustrating a wavelength of light passing through each section of a transmission type emission spectrometer according to the first embodiment.
FIG. 7 is a graph in which a wavelength graph of light passing through each section of the transmission type emission spectrometer according to the first embodiment is overlapped and compared.
FIG. 8 is a schematic diagram showing a configuration of a reflection type emission spectrometer according to the present invention.
Hereinafter, the present invention will be described in detail.
The present invention relates to a method of manufacturing a multi-wavelength monochromatic light source, comprising the steps of: (1) reflecting monochromatic light generated by a multi-wavelength monochromatic light source through a first color selection mirror;
The step of reaching the monochromatic light reflected in the step 1 to excite the target and obtain the transmissive light emission from the excited target (step 2);
Removing the monochromatic light other than the luminescent light by using the second color discriminating mirror and separating only the luminescent light (step 3); And
And a step of detecting and analyzing the light emitted by the detector in the step (3) by a detector (step 4).
Hereinafter, the transmission type emission spectroscopic analysis method will be described in detail for each step.
First, step 1 according to the present invention is a step of reflecting monochromatic light generated by a multi-wavelength monochromatic light source through a first color-separating mirror, and more particularly, a step of reflecting monochromatic light generated by a multi-wavelength monochromatic light source of blue, Is irradiated with the first color selecting mirror and then the monochromatic light irradiated to the first color selecting mirror is reflected to the target of the sample portion.
At this time, the color selecting mirror in the step 1 is a reflector in which a flat glass is coated with a non-metallic material and the interference is utilized. It is preferable to use a multiband type color discriminating mirror capable of selecting two or more single wavelength light (monochromatic light) by appropriately selecting and reflecting a part of the visible light by controlling the material, film thickness, number of layers, Do.
(Step 1 ') of condensing the reflected monochromatic light into an objective lens after performing step 1 according to the present invention.
At this time, the objective lens collects the monochromatic light reflected in the step 1 and plays a role of increasing the dose of the monochromatic light irradiated to the target in the sample part without dispersing the monochromatic light.
Next, the step 2 according to the present invention is a step of reaching the monochromatic light reflected in the step 1 to excite the target and to obtain the transmissive light emitted from the excited target, more specifically, the step 1 of the step 1 The monochromatic light reflected from the color selection mirror is irradiated to the target to excite the target and the excited target is stabilized by emitting light.
At this time, the emitted light is transmitted to the other side of the target surface, that is, the other side of the target, from which the irradiated monochromatic light reaches the target, and is emitted.
In addition, some of the monochromatic light irradiated to the target penetrates the target without exciting the target, and travels in the same traveling direction as the emitted light emitted from the target.
Next, the step 3 according to the present invention is a step of removing monochromatic light other than light emission by using a second color discriminating mirror and separating light emitted only from the mixed light of the monochromatic light other than the light emission and the light emission obtained in the step 2, The mixed light consisting of the light emitted from the target of step 2 and the monochromatic light transmitted without being able to excite the target is irradiated to the second color discriminating mirror so as to transmit and separate the emitted light so as to excite the target, It is a step of reflecting and removing as in the one-color selection mirror.
Next, step 4 according to the present invention is a step of detecting and analyzing the luminescence separated in step 3 with a detector.
In a transmission type emission spectroscopic analysis method according to the present invention, when a multi-emission spectroscopic analysis is performed by using a monochromatic light and a multi-wavelength color selective mirror by a multi-wavelength monochromatic light source, a monochromatic filter or monochromatic The luminescence measurements can be made without mechanical replacement of the single-wavelength color-separating mirrors to measure changes in the luminescence wavelengths emitted from the sample by the device and a range of monochromatic light.
Therefore, the transmission type emission spectroscopic analysis method of the present invention can be applied to the field of biotechnology and genetic engineering such as DNA structure analysis and DNA sequencing, the environmental field such as the content of organic matter or inorganic matter in water, The present invention can be useful not only in food and agriculture such as analytical chemistry, detection of luminescent material in food, but also in industry such as quantification of polymer compounds, film coating field and the like.
In addition,
A light source unit (1) generating monochromatic light;
A first
The
The second
And a detection unit (208) for detecting and analyzing the light emission (207) separated by the second color discriminating mirror (206).
Hereinafter, each configuration, function, and operation of a transmission type emission spectroscopic analyzer according to the present invention will be described in detail.
First, the
Next, the first
The color selection mirror according to the present invention preferably uses a multi-band type color selection mirror capable of selecting two or more single-wavelength range lights (monochromatic lights). For example, as shown in FIG. 3, when a multi-wavelength color discriminating mirror is irradiated with multi-wavelength light such as white light, the multi-band color discriminating mirror selectively reflects a plurality of specific wavelength range light (monochromatic light) And the light in the other range is transmitted. Due to this property, even when the wavelength of the
And an objective lens for collecting and transmitting the monochromatic light 202 'reflected by the first
At this time, the objective lens collects the monochromatic light 202 'reflected in the step 1 and increases the irradiation amount of the monochromatic light 202' irradiated to the target in the
Next, the
Next, the second
Next, the detecting
The transmission type emissive spectroscopic analyzer according to the present invention can be applied to a monochromatic filter for separating monochromatic light from a light source or a monochromatic filter for separating monochromatic light from a light source when monochromatic light and multi- Luminescence can be measured without mechanical replacement of a single-wavelength color-selective mirror in order to measure changes in the emission wavelength generated from the sample by the device and a range of monochromatic light.
Therefore, the transmission type emissive spectrometer of the present invention can be applied to the field of biotechnology and genetic engineering such as DNA structure analysis and DNA sequencing, the environmental field such as the content of organic matter or inorganic matter in the water, The present invention can be useful not only in food and agriculture such as analytical chemistry, detection of luminescent material in food, but also in industry such as quantification of polymer compounds, film coating field and the like.
Further, the present invention provides a method of manufacturing a color filter, comprising the steps of: (1) reflecting monochromatic light generated by a multi-wavelength monochromatic light source through a color selection mirror;
A step (step 2) of obtaining the reflective light emission emitted from the excited target by causing the monochromatic light reflected at the step 1 to reach the target to excite the target;
Removing the monochromatic light other than the luminescent light by using the color selection mirror of step 1 and separating only the luminescent light (step 3); And
And a step of detecting and analyzing the luminescence separated in the step 3 by a detector (step 4).
Hereinafter, the reflection type emission spectroscopic analysis method will be described in detail for each step.
The step 1 according to the present invention is a step of reflecting monochromatic light generated by a multi-wavelength monochromatic light source through a color selection mirror, and more particularly, a step of reflecting a monochromatic light emitted from a multi-wavelength monochromatic light source of blue, And a step of reflecting the monochromatic light irradiated on the color discriminating mirror to the target of the sample portion.
At this time, the color selecting mirror in the step 1 is a reflector in which a flat glass is coated with a non-metallic material and the interference is utilized. It is preferable to use a multiband type color discriminating mirror capable of selecting two or more single wavelength light (monochromatic light) by appropriately selecting and reflecting a part of the visible light by controlling the thickness of the material, film thickness, Do.
(Step 1 ') of condensing the reflected monochromatic light into an objective lens after performing step 1 according to the present invention.
At this time, the objective lens collects the monochromatic light reflected in the step 1 and plays a role of increasing the dose of the monochromatic light irradiated to the target in the sample part without dispersing the monochromatic light.
Next, the step 2 according to the present invention is a step of reaching the monochromatic light reflected in the step 1 to excite the target, and obtaining the reflection type light emitted from the excited target, more specifically, the step The monochromatic light reflected from the selection mirror is irradiated to the target to excite the target and the excited target is stabilized by emitting light.
At this time, the emitted light is reflecited by a path spatially coinciding with the path of the monochromatic light irradiated to the target.
In addition, some of the monochromatic light irradiated to the target penetrates the target without exciting the target, and travels in the same traveling direction as the emitted light emitted from the target.
Next, the step 3 according to the present invention is a step of removing monochromatic light other than light emission by using the color selection mirror of step 1, and separating only light emission, from mixed light of monochromatic light other than the light emission and light emission obtained in step 2, More specifically, mixed light composed of the light emitted from the target of step 2 and the monochromatic light reflected without being able to excite the target is irradiated to the color selection mirror of step 1 to transmit and separate the emitted light, The monochromatic light is reflected and removed as in step 1 above.
Next, step 4 according to the present invention is a step of detecting and analyzing the luminescence separated in step 3 with a detector.
The reflection type emission spectroscopic analysis method according to the present invention is a monochromatic filter for separating monochromatic light from a light source when multi-emission spectral analysis is performed by using a monochromatic light and a multi-wavelength color selective mirror by a multi-wavelength monochromatic light source, The luminescence can be measured without mechanical replacement of a single-wavelength color-selective mirror in order to measure the change in the luminescence wavelength generated from the sample by a monochromator and a wide range of monochromatic light.
Therefore, the reflection type emission spectroscopic analysis method of the present invention can be applied to the field of biotechnology and genetic engineering such as DNA structure analysis and DNA sequencing, the environmental field such as the content of organic matter or inorganic matter in water, Such as analytical chemistry such as food and agricultural fields such as detection of luminescent materials in foods, as well as quantitative determination of polymer compounds, film coatings, and the like.
Further,
A
A
A sample portion (304) comprising the target, wherein the monochromatic light (302) reflected from the color selection mirror (303) reaches a target and excites a target and the transmissive light (305) emits light from the excited target; And
And a
Hereinafter, each configuration, function and action of the reflection type emission spectrometer according to the present invention will be described in detail.
First, the
Next, the color-separating
The color selection mirror according to the present invention preferably uses a multi-band type color selection mirror capable of selecting two or more single-wavelength range lights (monochromatic lights). For example, as shown in FIG. 3, when a multi-wavelength color discriminating mirror is irradiated with multi-wavelength light such as white light, the multi-band color discriminating mirror selectively reflects a plurality of specific wavelength range light (monochromatic light) And the light in the other area is transmitted. Due to this property, even when the wavelength of the
And an objective lens for collecting and transmitting the
At this time, the objective lens converges the
Next, the
The
The reflection type emission spectroscopic analyzer according to the present invention can be used as a monochromatic filter for separating monochromatic light from a light source when multi-emission spectroscopic analysis is performed by using monochromatic light and multi-wavelength color selective mirror by a multi-wavelength monochromatic light source, The luminescence can be measured without mechanical replacement of a single-wavelength color-selective mirror in order to measure the change in the luminescence wavelength generated from the sample by a monochromator and a wide range of monochromatic light.
Therefore, the reflection type emission spectrometer of the present invention can be applied to the field of biotechnology and genetic engineering such as DNA structure analysis and DNA sequencing, the environmental field such as the content analysis of organic matter or inorganic matter in water quality, Such as analytical chemistry such as food and agricultural fields such as detection of luminescent materials in foods, as well as quantitative determination of polymer compounds, film coatings, and the like.
Hereinafter, the present invention will be described in detail with reference to examples.
However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.
Principle of sample analysis of transmission type emission spectrometer
The process of analyzing a sample using the transmission electron emission spectrometer according to the present invention can be understood by the following analysis example.
A spectrophotometer (SM240, Korea Spectral Products Co., Ltd.) for measuring the wavelength of light passing through each component of the transmission type emission spectrochemical analysis apparatus according to the present invention is provided in each section of the analyzing apparatus (for example, The positions of 202, 202 ', 202', 204, 207 and 209 of the transmission type luminescence analyzer are checked. The wavelength of light passing through each section is measured to confirm the operation principle of the transmission type emission spectrometer. Thereafter, the transmission type emission spectroscopic analysis (Rhodamin 6G) is fixed to the sample part and the light source part is operated to measure the sample (Rhodamin 6G) at about 505 nm to 560 nm suitable for analyzing the sample (Rhodamin 6G) only In order to confirm the effect of the second
Referring to FIG. 4, when the light 204 having an undesired wavelength exists in the
In addition, the second color-separating mirror can not excite the target by separating the monochromatic light 202 'reflected from the first color-separating mirror from the emitted light 207 emitted from the target, so that the transmitted monochromatic light 202' It is possible to prevent the
Referring to FIG. 6, the transmissive emission spectrometer according to the present invention uses a multi-wavelength monochromatic light source and a multi-wavelength color selective mirror to selectively irradiate monochromatic light with monochromatic light and monochromatic light having different wavelengths, It was confirmed that it can be easily carried out without mechanical movement of the apparatus (refer to the
In addition, the color selection mirror according to the present invention may be provided with
Further, as can be seen from the wavelength of the light reaching the
101: white light
102: first optical filter
103: monochromatic light
104: Plate
105: sample
106: objective lens
107: Light generated by the light emitting body of the
108: single band dichroi mirror
109: second optical filter
110:
201: Multi-wavelength monochromatic light source
202: monochromatic light
202 ': monochromatic light reflected from the first color selecting mirror
202 ": monochromatic light reflected from the second color selecting mirror
203: 1st color selection mirror
204: First color selection Monochromatic light transmitted from winter
205:
206: 2nd color selection mirror
207: Light generated by the light emitting body of the
208:
209: Light transmitted through the second color selecting mirror
301: Multi-wavelength monochromatic light source
302: monochromatic light
303: Color selection mirror
304:
305: Light generated by the light emitting body of the
306:
Claims (12)
The step of reaching the monochromatic light reflected in the step 1 to excite the target and obtain the transmissive light emission from the excited target (step 2);
Removing the monochromatic light other than the luminescent light by using the second color discriminating mirror and separating only the luminescent light (step 3); And
(Step 4) of detecting and analyzing the separated luminescence from the step 3 with a detector,
Wherein the color selection mirror of step 1 is a multi-band type color selection mirror capable of selecting at least two lights in a single wavelength range (monochromatic light).
Wherein the second color discriminating mirror is usable without replacement even when the emission wavelength generated from the target is changed.
Further comprising the step of condensing the reflected monochromatic light into an objective lens after the step 1 is performed (step 1 ').
A first color discriminating mirror for reflecting the monochromatic light generated from the light source unit;
A sample portion including the target, wherein the monochromatic light reflected from the first color selecting mirror reaches the target to excite the target and the transmissive light emitted from the excited target emits light;
A second color discriminating mirror for removing monochromatic light other than the luminescent light from the mixed light of the light emitted from the sample and the monochromatic light other than the luminescent light and separating only the luminescent light; And
And a detector for detecting and analyzing the light emission separated by the second color discriminating mirror,
Wherein the first color discriminating mirror is a multiband type color discriminating mirror capable of selecting at least two single wavelength range lights (monochromatic lights).
Further comprising an objective lens for collecting and transmitting the monochromatic light reflected from the first color-separating mirror to a sample unit.
A step (step 2) of obtaining the reflective light emission emitted from the excited target by causing the monochromatic light reflected at the step 1 to reach the target to excite the target;
Removing the monochromatic light other than the luminescent light by using the color selection mirror of step 1 and separating only the luminescent light (step 3); And
(Step 4) of detecting and analyzing the separated luminescence from the step 3 with a detector,
Wherein the color selection mirror of step 1 is a multi-band type color selection mirror capable of selecting at least two lights in a single wavelength range (monochromatic light).
Wherein the color selection mirror is usable without replacement even when the emission wavelength generated from the target is changed.
The method of claim 1, further comprising the step of condensing the reflected monochromatic light into an objective lens (step 1 ') after performing step 1).
A color discriminating mirror for simultaneously performing the function of reflecting the monochromatic light generated from the light source unit and the function of selecting only the reflection type light emitted from the sample unit;
A sample portion including the target, wherein the monochromatic light reflected from the color selection mirror reaches a target to excite the target and the transmissive luminescence is emitted from the excited target; And
And a detector for detecting and analyzing luminescence separated by the color selection mirror,
Wherein the color discriminating mirror is a multi-band color discriminating mirror capable of discriminating at least two lights in a single wavelength range (monochromatic light).
Further comprising an objective lens for condensing the monochromatic light reflected from the color discriminating mirror and transmitting the collected monochromatic light to a sample unit.
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| Application Number | Priority Date | Filing Date | Title |
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| KR1020130018027A KR101675258B1 (en) | 2013-02-20 | 2013-02-20 | Multi luminescence spectroscopic method using multi band dichroic mirror and luminescence spectrophotometer using the same |
| PCT/KR2014/001266 WO2014129774A1 (en) | 2013-02-20 | 2014-02-17 | Multi-emission spectrochemical analysis method using dichroic mirror and emission spectrometer using same |
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| KR1020130018027A KR101675258B1 (en) | 2013-02-20 | 2013-02-20 | Multi luminescence spectroscopic method using multi band dichroic mirror and luminescence spectrophotometer using the same |
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| JP2012058105A (en) | 2010-09-09 | 2012-03-22 | Dkk Toa Corp | Optical analyzer |
| WO2012104496A1 (en) | 2011-02-04 | 2012-08-09 | Horiba Abx Sas | Device and method for multiparametric measurements of microparticles in a fluid |
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| JP3102938B2 (en) * | 1991-12-30 | 2000-10-23 | シスメックス株式会社 | Particle image analyzer |
| US5907401A (en) * | 1997-09-03 | 1999-05-25 | The United States Of America As Represented By The Secretary Of The Army | Device and method for performing an optical hall test |
| JP3820806B2 (en) * | 1999-07-21 | 2006-09-13 | 三菱電機株式会社 | Laser inspection equipment |
| WO2012023816A2 (en) | 2010-08-18 | 2012-02-23 | 주식회사 나노엔텍 | Fluorescence microscope for multi-fluorescence image observation, fluorescence image observation method using the same, and multi-fluorescence image observation system |
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| JP2012058105A (en) | 2010-09-09 | 2012-03-22 | Dkk Toa Corp | Optical analyzer |
| WO2012104496A1 (en) | 2011-02-04 | 2012-08-09 | Horiba Abx Sas | Device and method for multiparametric measurements of microparticles in a fluid |
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