KR101584063B1 - Cosmetic composition for improving acne containing desalinized magma seawater and natural complex extract - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising desalinized magma seawater and a natural composite extract. Provided is a cosmetic composition comprising desalinized magma seawater; and a composite extract including a Machilus thunbergii extract, a Camellia japonica L. leaf extract, an Oryza sativa extract, and a Hijikia fusiforme Okamura extract as active components.

Description

Technical Field [0001] The present invention relates to a cosmetic composition for improving acne, which comprises a desalted lava sea water and a natural compound extract,

The present invention relates to a cosmetic composition for improving acne containing desalted lava sea water and a natural complex extract. More particularly, the present invention relates to a cosmetic composition for improving acne containing desalted seawater and a natural complex extract, As an active ingredient, and is excellent in anti-inflammatory and antibacterial effects.

Acne is an inflammatory disease that occurs in the skin of hair follicles. 70-80% of the patients are concentrated in the age group of 11-25 years, and they were previously thought to be specific diseases for adolescent adolescents. However, these days, It is one.

Acne can develop symptoms due to an increase in sebum secretion, hyperkeratosis-induced follicular stenosis, proliferation of acne bacterium, inflammatory reaction, etc., and various mechanisms may be combined and adversely affected .

Although excessive sebum secretion, hair follicle keratinization, and acne bacterium proliferation are the main causes of acne, the hyperplasia of sebaceous gland, hyperkeratosis of hair follicles, and bacterial effects interact to aggravate acne. Acne can be caused.

In addition, genetic factors such as acne-prone constitution, external factors such as season and climate, food effects, fatigue and stress are also related to acne. The cause of acne has not yet been clarified, but it is reported that acne can be directly induced by the above-mentioned main causes, if genetic factors and environmental factors are excluded.

Acne can be treated through excessive suppression of sebum secretion and relief of inflammation. Therefore, the occurrence of acne can be effectively suppressed by eliminating a complex cause that causes acne.

In general, medicines in the form of oral or topical agents may be used to remove the major cause of acne and treat acne. Specifically, the present invention relates to a method for inhibiting sebum production using antihistamines, an antimicrobial agent such as resorcinol, sulfur, salicylic acid, benzoyl peroxide, retinoic acid, and isotretinoin for antiinflammatory action of steroid hormones and nonsteroidal antiinflammatory agents, A method of inhibiting the activity of propionibacterium acnes by an antibiotic substance such as a keratolytic agent, tetracycline, erythromycin, and macrocycline.

However, despite the fact that the acne treatment method has shown some success in treating acne, it shows some problems in terms of effectiveness, side effects, and usability.

Specifically, hormones induce hormone abuse side effects such as inhibition of epidermal growth. Keratolytic agents such as retinoic acid and benzoyl peroxide can cause contact dermatitis due to skin irritation. Antibiotics such as tetracycline may cause resistant bacteria And may be a factor of photosensitizing action.

Meanwhile, in the cosmetics industry, many products using natural products have been developed to reduce skin irritation caused by various chemical substances. In addition to low adverse effects on skin, natural materials have recently become increasingly appreciated as a cosmetic raw material as consumers' response to cosmetics using natural materials has increased. However, the cosmetic product containing the natural product extract obtained by a conventional method is not practically effective because antibacterial and anti-inflammatory functions are not effectively achieved and the activity of the extract is not maintained and controlled continuously.

Therefore, in order to treat and improve acne, it is necessary to develop natural cosmetic products which are not only safe to human body but also have excellent skin improving effect.

However, when a single natural raw material is used, various kinds of effective ingredients for realizing the skin improvement effect may not be included, and thus it is suitable for the development of multi-functional cosmetics, which is gradually becoming a trend in the cosmetics industry Therefore, various studies have been made to improve this.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above-mentioned problems, and it is an object of the present invention to provide a cosmetic composition for improving acne originating from natural raw materials excellent in biosafety and excellent in anti-inflammation and antibacterial activity.

According to an aspect of the present invention, desalted lava water; And a complex extract composed of an extract of Lilac, a camellia leaf, a rice extract, and a top extract as an active ingredient.

According to one embodiment, the desalted lava waters may be included in an amount of 5.0 to 50.0% by weight based on the total weight of the composition.

According to one embodiment, the combined extract may be contained in an amount of 0.5 to 10.0% by weight based on the total weight of the composition.

According to one embodiment, the complex extract may be composed of 10-20 parts by weight of the extract, 10-120 parts by weight of a camellia flower leaf extract, 10-20 parts by weight of a rice extract, and 10-20 parts by weight of a top extract.

According to one embodiment, the combined extract comprises water, a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate. Butyl acetate, and 1,3-butyleneglycolic acid.

According to one embodiment, the alcohol may be ethanol at a concentration of 40 to 70%.

According to one embodiment, the combined extract can be extracted for 12 to 18 hours at a temperature of 50 ° C to 60 ° C using the ethanol as a solvent.

According to one embodiment, the cosmetic composition may be for improving acne.

According to one embodiment, the cosmetic composition may be at least one selected from the group consisting of softening agents, nutritional lotions, nutritional creams, massage creams, essences, eye creams, cleansing creams, cleansing foams, cleansing waters, packs, .

According to the present invention, the cosmetic composition contains an effective ingredient derived from a natural plant, so that it has excellent skin improving effect, particularly anti-inflammation and antibacterial effect.

In addition, the cosmetic composition can maximize the effect of improving the acne due to the synergistic action of the mineral component contained in the desalted lava sea water and the active ingredient contained in the plant extract.

It should be understood that the effects of the present invention are not limited to the above effects and include all effects that can be deduced from the detailed description of the present invention or the configuration of the invention described in the claims.

As used herein, the terminology used herein is intended to encompass all commonly used generic terms that may be considered while considering the functionality of the present invention, but this may vary depending upon the intent or circumstance of the skilled artisan, the emergence of new technology, and the like. Also, in certain cases, there may be a term selected arbitrarily by the applicant, in which case the meaning thereof will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term, not on the name of a simple term, but on the entire contents of the present invention.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.

The numerical range includes numerical values defined in the above range. All numerical limitations of all the maximum numerical values given throughout this specification include all lower numerical limitations as the lower numerical limitations are explicitly stated. All the minimum numerical limitations given throughout this specification include all higher numerical limitations as the higher numerical limitations are explicitly stated. All numerical limitations given throughout this specification will include any better numerical range within a broader numerical range, as narrower numerical limitations are explicitly stated.

Hereinafter, embodiments of the present invention will be described in detail, but it should be apparent that the present invention is not limited by the following examples.

According to an aspect of the present invention, desalted lava water; And a complex extract composed of an extract of Lilac, a camellia leaf, a rice extract, and a top extract as an active ingredient.

Since the cosmetic composition contains the above-mentioned complex extract as an active ingredient, it has excellent antimicrobial and anti-inflammatory activity and is highly effective in improving acne. In particular, the cosmetic composition of the present invention has a synergistic effect of each component contained in the complex extract, so that the skin improvement effect can be remarkably enhanced compared with a cosmetic composition containing the single extract. In addition, since the cosmetic composition contains desalted lava sea water as an active ingredient, the rich mineral contained in the desalted lava sea water and the active ingredient of the complex extract act simultaneously, thereby maximizing the skin improving effect.

The " extract " refers to a solvent in which an effective ingredient contained in an extraction material is transferred by bringing a solvent and an extraction material into contact under specific conditions. If the substance is obtained by extracting a component contained in a raw material from a natural product, Regardless of the type. For example, it may include a component obtained by extracting a component dissolved in a solvent from a natural product using water or an organic solvent, a specific component of a natural product such as a product obtained by extracting only a specific component such as oil, and the like.

The above-mentioned "Lava Seawater" is an underground seawater flowing into the ground under natural filtration of seawater by a permeable volcanic rock layer. It is mainly buried in the eastern part of Jeju. It flows up to 8km from the coast and passes through the volcanic rock layer It is known. The lava seawater is not mixed with each other due to the difference in density with freshwater groundwater, and exists below the freshwater layer.

Since the 1980s, Jeju has been used as a breeding ground for flounder farms by utilizing characteristics such as low temperature and cleanliness of lava seawater, and it has been widely spotlighted as being used as water for sauna and the like in terms of health and beauty. Lava seawater, which is distributed only in the eastern part of Jeju Island (Gucheong-eup, Seongsan-eup and Seokseon-myeon), is clean and has a constant water temperature throughout the year. It contains a large amount of various minerals not found in ordinary salt groundwater or seawater. It is not described in detail in this document, but has already been known in the existing documents (Patent No. 10-1402025 et al.). In particular, vanadium contained in trace amounts in lava seawater has been known to stabilize insulin secretion, and germanium has an effect on blood circulation promotion, immunity enhancement, anti-cancer effect, etc. Selenium inhibits oxidation of fat, Anti-cancer, infertility, suppression of aging, and improvement of cholesterol levels.

The lava seawater not only possesses abundant minerals but also has excellent activity suppressing activity and acne improvement activity of 5α-reductase and antihistamine activity due to degranulation inhibitory activity (β-hexosaminidase free inhibitory activity) And exhibits activity of promoting the production of type I collagen when treated with fibroblasts.

In addition, since the lava water is naturally purified in the process of passing through the basalt layer, it is very clean and unlike the deep sea water which is taken from the deep sea, the lava water is excellent in cost efficiency because it can be taken from the land.

The " desalted lava water " is a result obtained by desalting lava seawater, and may be a partially dehydrated (with some water removed) concentrated liquid phase or a completely dehydrated powder phase. The phase of the desalted lava waters is not particularly limited, and a composition containing minerals may suffice.

The desalted lava waters may be obtained by applying any technique known in the art and may include but are not limited to flash evaporation method, seawater freezing method, reverse osmosis method, ion exchange resin method, electrodialysis method, Can be appropriately selected in consideration of the characteristics. Specifically, the reverse osmosis method removes salt using a reverse osmosis membrane. Electrodialysis refers to a method of selectively lowering sodium concentration without changing the concentration of necessary minerals.

On the other hand, the complex extract may be composed of a Chinese cabbage extract, a Camellia sinica leaf extract, a rice extract, and a top extract. The complex extract has excellent skin improving effect due to the synergistic action of the active ingredients derived from each plant raw material and is applied to the skin together with the abundant minerals contained in the desalted lava sea water, Can be maximized.

The above-mentioned " Machilusthunbergii " is an evergreen broad-leaved arboreous tree with a height of 20m. The bark is brown with a bark eye, and a young branch is green. It is characterized by the bark falling like a piece of scales, the alternate leaves appear to have been turned at the end of the branch, are obovate or oblong, and the edges are flat. The surface is green, the vagina is thick, there are no hairs on both sides, the petiole is 2 ~ 3㎝ long and thick, and the flower is bisexual and the flower of yellowish green is on the cone flower from the axil of the leaf from May to June. Fruits are rounded into berries and ripened in black color from July to September of the following year. South Korea is native to Ulleungdo and southern coast, growing at the foot of the coast, generally weak in the cold but strong in my own good growth in fertile coastal regions. 4-O-methylhonokiol was first isolated from the bean curd and 4-O-methylhonokiol was reported to have antibacterial and anti-inflammatory activity together with Magnolol. Preferably the extract is obtained by drying dried bark of Magnolia officinalis Rehder et Wilson and M. obovata Thumb. Belonging to Magnoliaceae as an extraction raw material. The cookies include all the various organs of natural, hybrid, and variant, and include, for example, shells, embryos, and leaves, as well as plant tissue culture, and may include preferably shell capsules.

The above-mentioned " Oryzasativa L. " is an annual herb and is classified into rice, paddy field, and rice paddy depending on the viscosity of the rice paddy. Plant Classification Rice is one of the seed plants (an organ which becomes a seed after being formed in a fox) among the pizza plants surrounded by the fox. It also belongs to monocotyledonous cotyledons in the seed. Rice roots consist of seeds, midgut, and rootstocks, which absorb water and nutrients through the root hair. Rice grows and divides in the main stem and the primary tiller branch (the branches come out from the node in the ground of the plant). Stems consist of nodes and nodes. The leaves are divided into leaf sheath and leaf sheath, with leaf veins and leaf ears in between. Rice contains about 75% starch, about 8% gray matter, 0.5-1% fat and a small amount of vitamin B. Wherein said fat is selected from the group consisting of ester cholesterol and free cholesterol, brassicasterol, stigmasterol, sitosterol, monoglyceride, diglyceride, triglyceride, phospholipid, Contains free fatty acids.

The above " rice extract " refers to an extract prepared from the leaves and stems of rice, and leaves and stems of rice can contain an active ingredient exhibiting anti-inflammatory and antibacterial activity, And plant tissues of rice including both roots as an extraction raw material.

The " Camellia japonica " is an evergreen tall tree belonging to the genus Camellia and is called camellia or camellia. Camellia leaves are alternate phyllotaxis, elliptical or elongated elliptical, with both ends pointed, leaf surface dark green, glossy, and yellowish green on the back. The camellia has been known to have anti-inflammatory and antimicrobial activity, and its extracts of seed and peel have been reported to have anti-inflammatory and anti-inflammatory activity and stem extracts have antioxidant effects. Camellia japonica contains proline and glutamine as its main amino acids and other trace amounts of free amino acids. In particular, camellia leaves have antimicrobial activity against malassezia causing scalp disease and have been known to be effective in improving scalp disease. In addition, Camellia sinensis leaf extract showed strong antibacterial activity and excellent antimicrobial activity against food poisoning bacteria and Staphylococcus aureus was confirmed.

The " Hizikia " fusiformis "is a natural resource plant inhabiting the western coast of Korea, the southern coast of Korea, and Jeju Island. It contains a large amount of laminaran, a neutral polysaccharide, which has abundant dietary fiber and functions such as blood coagulation and immunity, and a sulfated polysaccharide, fucoidan. It contains various physiologically active substances and is known to have excellent antioxidative and antimicrobial activity and high content of minerals such as calcium, and thus has excellent efficacy in the prevention and treatment of various adult diseases and chronic diseases.

On the other hand, the desalted lava water may be contained in an amount of 5.0 to 50.0% by weight based on the total weight of the composition, and the combined extract may be included in an amount of 0.5 to 10.0% by weight based on the total weight of the composition.

If the content of the desalted lava waters is less than 5% by weight, the content of the lava water is insufficient and the anti-inflammatory activity due to minerals may not be sufficiently exhibited. If the content is more than 50% by weight, Is relatively small, the synergistic effect due to the interaction may not be properly realized.

If the content of the complex extract is less than 0.5% by weight, the effect of improving the skin due to the complex extract may not be fully realized. If the content is more than 10.0% by weight, The extract may be contained in the cosmetic composition so that the original quality of the cosmetic composition may not be realized.

According to one embodiment, the complex extract may be composed of 10-20 parts by weight of the extract, 10-120 parts by weight of a camellia flower leaf extract, 10-20 parts by weight of a rice extract, and 10-20 parts by weight of a top extract.

When the deviation of the mixing ratio of the complex extract is large, the antimicrobial activity and the acne improvement effect derived from the active ingredient of each extract may not be suitably realized. Therefore, the mixing ratio is suitably controlled in consideration of the working environment and the quality of the final product can do. Particularly, the types of the active ingredients contained in the complex extract are very various, and the antimicrobial and anti-inflammatory activities may be different according to the mixing ratio of the complex extract, so that the additive effect due to simultaneous application of each extract may be maximized It is preferable to maintain an appropriate mixing ratio.

On the other hand, in the extraction step of each raw material, the kind of the solvent is not particularly limited, and various solvents may be used as needed. Further, the extraction step may be carried out by a conventional method in the art, such as cold-rolling, warming, heating, and the like using the solvent.

According to one embodiment, the combined extract may be extracted with one or more solvents selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol.

According to one embodiment, the combined extract is washed with water, dried, pulverized, and then subjected to circulation extraction with circulation for about 1 hour to 24 hours with a volume of 8 to 12 times the weight of each raw material, , Extraction with an ordinary method such as ultrasonic extraction, and filtration. In addition, the extract may be obtained in powder form by an additional process such as vacuum distillation or lyophilization.

On the other hand, the extract may also include an extract obtained through a conventional purification process. For example, the extract may be subjected to various purification methods such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity) Lt; RTI ID = 0.0 > fractions. ≪ / RTI >

According to one embodiment, the combined extract may be extracted at a temperature of 50 ° C to 60 ° C for 12 to 18 hours using ethanol as a solvent at a concentration of 40 to 70%.

That is, the extraction ratio may be different depending on the polarity of the solvent, and the ethanol is excellent in the selectivity in the extraction of the physiologically active substance of the natural raw material. Therefore, Can be implemented.

Particularly, the water and ethanol have different polarities, and the effective components extracted according to the respective polarities may be different from each other, so that the concentration of the ethanol can be appropriately controlled so that an optimum skin improving effect can be realized. However, when the concentration of the ethanol is more than 70%, an adequate yield may not be realized. If the concentration is less than 40%, the effective ingredient showing skin improving effect may not be extracted sufficiently. Therefore, Can be adjusted appropriately.

On the other hand, if the extraction temperature is higher than 60 ° C., the active ingredient contained in the natural raw material may be deformed or disappear at high temperature to lower the skin improving effect. If the extraction temperature is lower than 50 ° C., Therefore, it is preferable to keep the extraction temperature within an appropriate range. If the extraction time is less than 12 hours, the active ingredient contained in the natural raw material may not be completely extracted. If the extraction time exceeds 18 hours, the content of the remaining active ingredient contained in the natural raw material is inadequate, It is preferable to control the extraction time appropriately.

According to one embodiment, the cosmetic composition may be for improving acne.

The cosmetic composition is remarkably excellent in the effect of improving the acne due to the synergistic action of the mineral component contained in the desalted lava water and the active ingredient contained in the plant extract.

In particular, since the cosmetic composition contains both of the desalted seawater and the complex extract, the anti-inflammatory activity, antimicrobial activity, and skin safety are remarkably increased as compared with the case where the active ingredient is contained individually or partially.

According to one embodiment, the cosmetic composition may be at least one selected from the group consisting of softening agents, nutritional lotions, nutritional creams, massage creams, essences, eye creams, cleansing creams, cleansing foams, cleansing waters, packs, .

The cosmetic composition of the present invention may be appropriately blended with other ingredients within the range of not compromising the object of the present invention depending on the kind of the formulation or the purpose of use, in addition to the essential ingredients in each formulation.

In addition, the cosmetic composition may be a lipid, an organic solvent, a solubilizing agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, Surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, Any other ingredient used may also additionally contain adjuvants commonly used in the cosmetics or dermatological sciences.

However, it is preferable that the adjuvant and the mixing ratio thereof are appropriately selected so as not to affect the preferable properties of the cosmetic composition according to the present invention.

The present invention will be further described with reference to the following examples, but it should be apparent that the present invention is not limited by the following examples.

Manufacturing example  1: desalination Lava  Produce

Lava seawater taken from a specific area was filtered through a filter of 0.2 μm to remove monovalent ions from the ion exchange membrane of an electrodialyzer (ASTOM CO. LTD., Japan) to desalinate the desalted lava water Respectively.

Manufacturing example  2: Preparation of complex extract

Dried, and then pulverized to obtain 100 g of the pulverized product, respectively.

(50% mass concentration) of 10-fold volume of each of 100 g of each of 100 g of each of the crushed, ground, leaf, stem, and crumbs of the leaves were subjected to immersion extraction at a temperature of 50 ° C for 12 hours. Each of the extracts was filtered by a filter, and the extracts obtained by repeatedly extracting the remaining raw materials three times in the same manner were mixed to complete a complex extract. The combined extracts were concentrated under reduced pressure at 30 DEG C and then lyophilized to give a solid.

The obtained complex extract was put into a cosmetic composition, and the skin improving effect on the cosmetic composition was verified.

Example : Complex extract and desalting Lava seawater  Mixed sample

The lyophilized lava waters prepared in Preparation Example 1 obtained by lyophilization and the combined extracts obtained in Preparation Example 2 were mixed with each other in the same ratios as the extracts of Capsicum annuum extract, Camellia sinensis leaf extract, Rice extract and Top extract.

Comparative Example  1: desalination Lava seawater  sample

A part of the desalted lava waters of Production Example 1 obtained by freeze-drying was separated to obtain a sample of Comparative Example 1.

Comparative Example  2: Compound extract sample

A part of the combined extract obtained by freeze-drying and obtained in Preparation Example 2, the extract of Camellia sinensis leaf, the extract of rice, and the extract of top extract was fractionated and used as a sample of Comparative Example 2.

Comparative Example  3: Flavor extract sample

A part of the crude extract of Preparation Example 2 obtained by freeze-drying was separated and used as a sample of Comparative Example 3.

Comparative Example  4: Camellia sinensis leaf extract sample

A part of the camellia leaf extract of Preparation Example 2 obtained by lyophilization was isolated and used as a sample of Comparative Example 4.

Comparative Example  5: Rice extract sample

A part of the rice extract of Preparation Example 2 obtained by freeze-drying was separated and used as a sample of Comparative Example 5.

Comparative Example  6: Sample of extract

The lyophilized extract of Preparation Example 2 obtained by lyophilization was separated and used as a sample of Comparative Example 6.

Experimental Example  1: Assessment of cytotoxicity

The toxicity of the samples obtained in Example 1 and Comparative Examples 1 to 6 to cells was measured. After the samples obtained in Example 1 and Comparative Examples 1 to 6 were suspended in purified water and adjusted to concentration (100, 50, 25 μg / mL), cell viability was measured by the following method.

Cytotoxicity was performed by modifying the method of Mosmann to measure cell viability using MTT {3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide} reagent.

HDF was dispensed into 96-well plates at a concentration of 1 x 10 ⁴ cells / well and cultured at 37 ° C and 5% CO ₂ for 24 hours. The culture medium was removed and the sample was cultured for 24 hours in a concentration-treated medium. The medium was removed and washed twice with PBS (phosphate buffered saline). MTT was dissolved in PBS at 5 mg / mL and 50 L was added. The cells were cultured at 37 ° C and 5% CO ₂ for 2 hours. 100 L of DMSO (dimethyl sulfoxide) was added per well, and the mixture was stirred for 10 minutes and absorbance was measured at 540 nm.

As a result of the measurement, no cytotoxicity was observed at all concentrations of each sample. From this, it was confirmed that the desalted lava sea water and the complex extract were harmless to the human body and had excellent safety.

Experimental Example  2: Evaluation of anti-inflammatory activity (NO production Inhibition  analysis)

In order to determine the anti-inflammatory activity of the samples obtained in Example 1 and Comparative Examples 1 to 6, inhibition of NO production by EAME was analyzed in mouse macrophage RAW 264.7 cells stimulated with Lipopolysaccharide (LPS).

Raw 264.7 cells, a Murine macrophage cell line, were purchased from Korean Cell Line Bank and cultured in DMEM medium containing 100 units / mL penicillin-streptomycin and 10% fetal bovine serum (FBS) at 37 ° C in a 5% CO ₂ incubator And subculture was performed once every 2 to 3 days.

Raw 264.7 cells (3 × 10 ⁵ cells / mL) were cultured 18 hours before and the extract samples (100, 50, 25 μg / mL) and LPS (1 μg / mL) according to Examples and Comparative Examples 1 to 6 The cells were cultured for 24 h at the same time, and the produced NO was measured in the form of NO ₂ ^- present in the cell culture using a Griess reagent. The negative control group was not treated with LPS, and the positive control group was treated with LPS alone to measure the amount of NO produced.

100 μL of cell culture supernatant was mixed with the same amount of Griess reagent (1% (w / v) sulfanilamide, 0.1% (w / v) naphtylehtylenediamine in 2.5% (v / v) phosphoric acid) Absorbance was measured at 540 nm using a reader. Standard concentration curves were obtained by serial dilution of sodium nitrite (NaNO ₂ ) (10-100 μM).

As shown in Table 1, the combined extract and desalted sea water mixed samples according to the examples showed higher NO (nitric oxide) production inhibitory activity at all concentrations, and the antioxidative and anti-inflammatory activities were remarkably excellent.

[NO production amount (μM)] division 25 μg / mL 50 μg / mL 100 μg / mL Control group LPS (-) LPS (+) Example 15.74 12.79 10.21 5.17 35.95 Comparative Example 1 25.34 22.67 21.18 Comparative Example 2 24.57 23.23 22.19 Comparative Example 3 28.65 27.27 27.10 Comparative Example 4 29.31 29.54 28.84 Comparative Example 5 30.31 28.88 26.23 Comparative Example 6 28.23 27.51 25.39

Experimental Example  3: Antimicrobial activity evaluation

The antimicrobial activity of the samples obtained in Example 1 and Comparative Examples 1 to 6 against acne bacteria was evaluated. Propionibacterium acnes ATCC 6919 was used as a test strain and triclosan, a chemical synthetic antibiotic, was used as a positive control.

Staphylococcus aureus was striking in tryptic soy agar medium containing 5% sheep blood and cultured for 2 ~ 3 days. The cells were cultured in McFaland nephleometer standards 0.5 (1% Barum chloride (0.05 ml) + 1% Sulfuric acid (9.95 ml)), the bacterial concentration was 1 × ^{10 8} cells / ml.

Each sample was diluted in 2 ml of liquid BHI medium at intervals of 100 ppm from 0 to 2000 ppm, followed by inoculation with 20 μl of 1 × ^{10 8} cells / ml of the bacterial solution, followed by incubation for 48 hours. The minimum inhibitory concentration (MIC) was defined as the lowest growth inhibitory concentration based on turbidity.

division Minimum growth inhibitory concentration MIC (PPM) Example 500 Comparative Example 1 1600 Comparative Example 2 1000 Comparative Example 3 1800 Comparative Example 4 3100 Comparative Example 5 2500 Comparative Example 6 1400 Triclosan 400

As shown in Table 2, the antibacterial effect of the combined extract and desalted sea water mixed sample according to the examples was slightly weaker than that of the positive control triclosan, but the antimicrobial effect was remarkably superior to those of Comparative Examples 1 to 6 .

Experimental Example  3: Evaluation of acne improvement effect

To evaluate the effect of the cosmetic composition containing the samples obtained in Example 1 and Comparative Examples 1 to 6 on acne improvement, the following experiment was conducted.

First, each of the samples was formulated in the same manner as shown in Table 3 below, and samples of Example 1 and Comparative Examples 1 to 6 were different.

[Content (% by weight)] division Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Desalted lava sea water (Preparation Example 1) 10.0 15.0 - - - - - The complex extract (Preparation Example 2) 5.0 - 15.0 - - The crude extract (Preparation Example 2) - - - 15.0 - - - Camelliaceae leaf extract (Preparation Example 2) - - - - 15.0 - - Rice extract (Preparation Example 2) - - - - - 15.0 - Top extract (Preparation Example 2) - - - - - - 15.0 1,3-butylene glycol 6.0 6.0 6.0 6.0 6.0 6.0 6.0 glycerin 4.0 4.0 4.0 4.0 4.0 4.0 4.0 Oleyl alcohol 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Polysorbate 20 0.5 0.5 0.5 0.5 0.5 0.5 0.5 ethanol 15.0 15.0 15.0 15.0 15.0 15.0 15.0 Benzophenone-9 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Incense, preservative a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount Purified water Balance Balance Balance Balance Balance Balance Balance

Of the women aged 19 to 26 years old, 50 persons with acne were allowed to use the formicized lotion of Example 1 and Comparative Examples 1 to 6 for 4 weeks in the same manner as the conventional lotion, .

division Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 1 week + ± ± ± ± ± ± 2 weeks ++ + ± ± ± ± ± 3 weeks +++ ++ + ± + ± ± 4 weeks +++ ++ + ++ + + +

<Evaluation Criteria>

+++: very good improvement effect / ++: considerable improvement effect / +: slight improvement effect / ±: not improvement effect but not deteriorating / -: improvement effect but rather weakness

As shown in Table 4, it was confirmed that the lotion (Example) including the combined extract and the desalted seawater mixed sample was significantly superior to the lotion containing the samples of Comparative Examples 1 to 6 in improving acne.

Experimental Example  4: Skin hazard assessment

The human health hazards of the cosmetic composition containing the samples obtained in Example 1 and Comparative Examples 1 to 6 were evaluated. Each sample was formulated according to Table 3, and a human patch test was performed on the formulated cosmetic composition. The test was conducted according to the CTFA guidelines (The Cosmetic, Toiletry and Fragrance Association, Inc. Washington, D. D., 20036, 1991) for 30 healthy women aged 20-30 years.

For this test, 20 μl of a test substance (10% in patch base) prepared in a Finn chamber was dripped, washed with 70% ethanol, dried and fixed with a micropore tape . After 24 hours, the fan chamber was removed, and skin changes were read visually. The results are shown in Table 6 below.

In Table 5, the average degree of reactivity was calculated according to the following formula (1), and the criterion was grade I (unstimulated range) was less than 0.1, class II (light stimulus range) was less than 0.3, grade III , Grade Ⅳ (range of strong stimulus) was at least 0.55.

[Equation 1]

Average reactivity = [(±) number x 1 + (+) number x 2 + (++) number x 3] / number of subjects

Test substance Judgment result Average reactivity Judgment grade - ± + ++ Example 29 One 0 - 0.07 Ⅰ Comparative Example 1 27 2 One - 0.13 Ⅱ Comparative Example 2 27 One 2 - 0.13 Ⅱ Comparative Example 3 27 2 One - 0.13 Ⅱ Comparative Example 4 26 2 2 - 0.20 Ⅱ Comparative Example 5 26 3 One - 0.17 Ⅱ Comparative Example 6 26 3 One - 0.17 Ⅱ

<Evaluation Criteria>

-: No irritation or unusual phenomenon / ±: Slightly reddened from surrounding area / +: Significantly reddened from surrounding area / ±: No improvement effect but no deterioration / ++:

As shown in Table 5, the cosmetic compositions formulated in the samples of Comparative Examples 1 to 6 exhibited a stimulus value in a safe range, but the stimulus was relatively high. On the other hand, the cosmetic composition formulated in the examples showed a grade I irritation, which was lower than the safety level of grade II (stimulation value less than 0.3).

That is, the lotion (Examples) including the complex extract and the desalted sea water mixed sample according to one embodiment of the present invention has little skin irritation and can be commercialized into various formulations, and the functionality, cost and other It is possible to control the content at a proper ratio in consideration of the conditions.

According to one embodiment, a cosmetic containing the desalted lava sea water and complex extract of the present invention can be produced according to the following formulation examples.

Formulation Example  1: Lotion Cosmetics

The ingredients shown in Table 6 including the oil component and the water component were heated and mixed at 75 캜 for emulsification and then cooled to room temperature to prepare a lotion cosmetic containing the desalted lava water and the combined extract of the present invention.

ingredient weight% The complex extract of the present invention 5.00 Desalted lava sea water 10.00 glycerin 3.00 antiseptic 0.50 Desalted lava sea water 10.00 Allantoin 0.05 DL-Panthenol 0.05 Sheer butter 2.00 Jozova Esther 0.50 Beads wax 0.50 Behenic Acid 0.50 Cetyl ethyl hexanoate 4.50 Cetearyl olivate 2.00 Jojoba oil 4.00 Purified water Balance incense 0.20 Sum 100

Formulation Example  2: Cream Cosmetics

The ingredients of Table 7 including the oil component and the water component were heated and mixed at 75 캜 to emulsify and then cooled to room temperature to prepare a cream cosmetic containing the desalted lava sea water and complex extract of the present invention.

ingredient weight% Complex extract 3.00 Desalted lava sea water 7.00 Cyclopentasiloxane 4.00 Desalted lava sea water 10.00 Dimethicone 5.00 glycerin 5.00 Stearic acid 4.00 Sheer butter 1.20 10-dimethicone crosspolymer 3.00 Betaine 0.50 Hydrogenated starch hydrolyzate 0.50 1,2-hexanediol 2.00 Olive oil 0.20 Jojoba seed oil 0.20 Allantoin 0.10 Xanthan gum 0.10 Tocopheryl acetate 0.05 Sodium hyaluronate 0.30 incense 0.20 Purified water Balance Sum 100

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (9)

Desalted lava sea water; And
10 to 20 parts by weight of the extract, 10 to 20 parts by weight of a camelliaceae leaf extract, 10 to 20 parts by weight of a rice extract, and 10 to 20 parts by weight of a top extract.
The method according to claim 1,
Wherein the desalted lava water is contained in an amount of 5.0 to 50.0% by weight based on the total weight of the composition.
The method according to claim 1,
Wherein the complex extract is contained in an amount of 0.5 to 10.0% by weight based on the total weight of the composition.
delete The method according to claim 1,
Wherein the combined extract is extracted with at least one solvent selected from the group consisting of water, a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol.
6. The method of claim 5,
Wherein the alcohol is ethanol at a concentration of 40 to 70%.
The method according to claim 6,
Wherein the complex extract is extracted for 12 to 18 hours at a temperature of 50 캜 to 60 캜 using the ethanol as a solvent.
The method according to any one of claims 1 to 3 and 5 to 7,
A cosmetic composition for improving acne.
The method according to any one of claims 1 to 3 and 5 to 7,
Wherein the cosmetic composition is formulated with at least one selected from the group consisting of softening agents, nutritional lotion, nutritional cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder.