KR101393007B1 - Cosmetic Composition for Skin-aging Protection and Wrinkle Improvement Comprising the Extract of Nephelium lappaceum and Litchi chinensis sonn as Active Ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing skin aging and improving wrinkles of skin, which contains rambutane and a rich extract as an active ingredient. And the cosmetic composition is applied to human skin. The rambutane and rich extract of the present invention exhibited excellent anti-aging effects such as an inhibitory effect on MMP-1 production, an effect of promoting collagen synthesis, and an antioxidant effect. In addition, the cosmetic composition containing the rambutane and the rich extract of the present invention has an excellent effect for improving wrinkles and skin wrinkles caused by skin aging.

Lambutan, rich extract, collagen synthesis, anti-aging, wrinkle improvement, MMP-1

Description

[0001] The present invention relates to a cosmetic composition for preventing skin aging and improving wrinkles of skin, which comprises as an active ingredient, an antibacterial agent, an antibacterial agent,

The present invention relates to a cosmetic composition for preventing skin aging and improving wrinkles of skin, which contains an extract of Nephelium lappaceum and Litchi chinensis sonn as active ingredients.

The skin of the human skin consists largely of the epidermis, the dermis, and the subcutaneous fat layer. The basic role of the skin is to prevent water from evaporating from our body and to prevent harmful substances from invading from the outside. These skin are always exposed to external stimuli, so they have various defenses. In addition, skin is a sign of beauty, and it can be said that skin is healthy and clean is related to human beauty. In recent years, not only women but also men have been interested in various skin troubles such as skin aging, and a lot of related products are being developed.

As a person gets older, skin aging occurs. Wrinkles are a typical symptom. Wrinkles are a phenomenon of age, and the most common cause of wrinkles is the degradation of collagen that forms the matrix in the dermis of the skin. Skin collagen can be reduced by aging, but the activity of collagenase (Matrix Metallo Proteinase, MMP-1) is increased by external stimuli such as ultraviolet rays, and collagen is easily decomposed to increase the generation of wrinkles There are many clinical reports. Therefore, in recent years, many products have been developed which inhibit the inhibition of the production of MMP-1 and the activity thereof, thereby suppressing the decomposition of collagen, which is basically produced.

On the other hand, MMP is a calcium and zinc-dependent endopeptidase secreted from cells such as polymorphonuclear neutrophils, macrophages, fibroblasts, etc., and acts at neutral pH, It uses various cell substrates. MMP-1, MMP-2, and MMP-9 are the most active enzymes in the skin, and the most fundamental enzyme involved in collagen degradation is MMP-1. In general, there are retinoids (RE36068), TGF-beta (Transforming growth factor), betulinic acid (JP8-208424), and the like, which inhibit the production of MMP- Transforming growth factor (β) is known to be known, and various natural products are known to inhibit MMP-1 biosynthesis. Therefore, the method of inhibiting wrinkle formation can prevent aging of the skin by promoting collagen synthesis or inhibiting the production and activity of collagenase, and there is a desperate need to develop cosmetics for more effective, easy and safe wrinkle improvement Is required.

Accordingly, the present inventors have made extensive efforts to develop a cosmetic composition that can exert an excellent effect on the wrinkle-reducing function and can be applied easily, and more safely to the skin. As a result, it has been found that the antioxidative effect, the synthesis of collagen, It is possible to obtain a more effective wrinkle-reducing effect by applying the active ingredient to the extract of the present invention.

Accordingly, it is an object of the present invention to provide a cosmetic composition for preventing skin aging which contains rambutane and a rich extract as an active ingredient.

An object of the present invention is to provide a cosmetic composition for improving skin wrinkles containing rambutane and a rich extract as an active ingredient.

Another object of the present invention is to provide a cosmetic method characterized by coating the cosmetic composition containing the above-mentioned lamambutan and rich extract on human skin.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention and claims.

The present invention relates to a cosmetic composition for preventing skin aging and improving wrinkles of skin, which contains rambutane and a rich extract as an active ingredient. And the cosmetic composition is applied to human skin. The rambutane and rich extract of the present invention exhibited excellent anti-aging effects such as an inhibitory effect on MMP-1 production, an effect of promoting collagen synthesis, and an antioxidant effect. In addition, the cosmetic composition containing the rambutane and the rich extract of the present invention has an excellent effect for improving wrinkles and skin wrinkles caused by skin aging.

The present invention relates to a cosmetic composition for preventing skin aging and improving wrinkles of skin, which contains rambutane and a rich extract as an active ingredient.

Nephelium lappaceum, which is an active ingredient in the cosmetic composition of the present invention, is the origin of the dicotyledonous rootstock of the Japanese persimmon tree. Generally, it grows about 10 ~ 15m in height in the tropical region. The rambutan fruit looks like a chestnut before it gets ripe and has a red color when ripe. Leaves are alternate phyllotaxis of 5 to 7 pairs, with hairs on the back when young. Flowers are yellowish green in April to June, with 5 petals.

Fruits are elliptical, barky, and have protuberances. When ripened in July ~ August, the taste is sweet and sour. In fruit, seeds are 2 ~ 3cm yellow oval shape. The bark and roots are used as toothpaste in the tropics.

The Litchi chinensis sonn used as an active ingredient of the cosmetic composition of the present invention is an evergreen arborescent tree of the perennial plant of the dicotyledonous plant, the southern part of China, and the distribution area is distributed widely in China. The size of the trees grows up to 10 ~ 15m in height. Rich is also called open-ended.

It is often grown in China. Flowers are yellowish green and calyx is composed of 4 ~ 10 pieces. The fruit is round, about 3cm in diameter, and looks like a turtle's back with a protuberance on the outside. The fruit has a bark, and the seeds are present in the shape of a reddish-brown round one to three centimeters in the fruit.

As used herein, the term " rambutane and rich extract " means obtained from various organs or parts such as leaves, flowers, roots, stems, branches, bark and fruits, seeds, Fruit, seeds, branches or roots, and more preferably means extracts derived from fruit husks, fruits and seeds.

Preferably, the rambutane and rich extracts of the present invention comprise (a) water, (b) anhydrous or lower alcohol having 1 to 4 carbon atoms such as methanol, ethanol, propanol and butanol, Ethyl acetate, (e) chloroform, (f) butyl acetate and (g) 1,3-butylene glycol as the extraction solvent. The extraction solvent is preferably a functional lower alcohol, more preferably 50-95% alcohol, most preferably 70% alcohol. A suitable amount of the extraction solvent is 1-20 times, and more preferably 5-10 times, the dry weight of the fruit of rambutane and rich.

In addition, "rambutane and rich extract" can be obtained by mixing rambutane and rich, or by mixing individual extracts of respective raw materials, and there is little difference in the effective ingredient depending on the extraction method of mixed extraction or individual extraction and mixing. In this case, the content ratio (weight ratio) of rambutane to rich is 1: 3 to 3: 1, preferably 2: 3 to 3: 2, and most preferably 1: 1.

  According to a specific embodiment of the present invention, the extraction is carried out as follows. First, lambutan and rich fruit are washed with purified water, dried and crushed into small pieces, and then the above extraction solvent is added in an amount of 1-10 times the dry weight. Then, it is extracted by heating at 40-100 ° C for 3 to 20 hours or at 4-40 ° C for 1 to 15 days in a state where a cooling condenser is installed to prevent evaporation of the active ingredient, and the resultant is thoroughly dried by a rotary evaporator, . In the case of 1,3-butylene glycol, it is difficult to dry using 1,3-butylene glycol using a rotary evaporator. Therefore, it is prepared by directly adjusting the amount of drying to 1% (w / v).

It is obvious to those skilled in the art that the extract of the present invention can be obtained not only with the above-mentioned extraction solvent but also with another extraction solvent, that exhibits substantially the same effect.

In addition, the extract of the present invention includes not only the extract obtained by the above-mentioned extraction solvent, but also an extract obtained through a conventional purification process. For example, by separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies (made for separation by size, charge, hydrophobicity or affinity), and the like, The fraction is also included in the rambutane and rich extract of the present invention. It is included in the form of extract by supercritical extraction and ultrasonic extraction which are usual device extraction methods.

The rambutane and rich extract of the present invention can be prepared in powder form by an additional process such as vacuum distillation and freeze drying or spray drying.

The cosmetic composition of the present invention may contain other skin wrinkle improving ingredients other than the above-mentioned rambutane and rich extract as effective ingredients. However, the cosmetic composition of the present invention exhibits an excellent skin wrinkle-improving effect even as an active ingredient composed of only a lamba-botan and a rich extract.

According to a preferred embodiment of the present invention, the content of the lampoban and the rich extract is 0.00001-30% by weight, preferably 0.0001-20% by weight, more preferably 0.01-10.0% by weight, Preferably 0.1-5.0 wt%.

At this time, when the content of the lambutan and the rich extract contained in the cosmetic composition is less than the minimum content, the expected effect is hard to appear, and when the content exceeds the maximum content, the effect is not further increased and the skin is likely to be stimulated. It also has a great influence on stabilization.

The ingredients contained in the cosmetic composition of the present invention include, in addition to the above-mentioned effective ingredients, the ingredients conventionally used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, vitamins, And a carrier.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

The present invention also provides a makeup method characterized by applying a cosmetic composition containing a lampoban and a rich extract of the present invention to human skin.

The cosmetic process of the present invention refers to all the cosmetic processes for applying the cosmetic composition of the present invention to human skin. That is, all methods known in the art for applying the cosmetic composition to the skin belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in combination, or may be used by overlapping with other cosmetic compositions other than the present invention. In addition, the cosmetic composition having excellent skin protection effect according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied depending on the skin condition or taste of the user.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

The inventive cosmetic composition comprising a humectant and a rich extract of the present invention is applied to a skin of a human, and thus it is possible to prevent skin aging and improve skin wrinkles due to excellent MMP-1 production inhibiting effect, collagen synthesis enhancing effect and antioxidant effect Can be obtained.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Production Example 1: Preparation of rambutane and rich extract I

Washed with purified water and dried, and 50.0 g of each of the dried and unrefined lamobutan and rich fruits were placed in 1.2 L of water and heated at 70-90 ° C. for 5 hours in an extractor equipped with a cooling condenser, After aging at -10 ° C for 7-10 days, the mixture was filtered through Wattman filter paper No. 5. The filtrate was dried at 65 ° C with a rotary attenuator to obtain dry weights of 15.1g (Preparation 1-1) and 13.5g (Preparation 1-2, Rich extract), respectively. The dried powder was dissolved in 1% (w / v) of 50% 1,3-butylene glycol solution and used in the present invention.

Preparation Example 2: Preparation of a lambutan extract II

50.0 g of each of the lambutan and rich fruit washed with purified water was added to 1.2 L of water, and the active ingredient was extracted at 15-35 ° C for 5 days. Then, the active ingredient was filtered through a 300-mesh filter cloth, And concentrated twice with a rotary evaporator. To this, 0.6 L of 100% ethanol was added, and the mixture was allowed to stand for 5-10 days at 5-10 ° C., followed by aging, followed by filtration through Wattman filter paper No. 5. The filtrate was dried with a rotary attenuator at 65 ° C to obtain 15.5 g (Preparation Example 2-1, Rambutane) and 14.5 g (Preparation Example 2-2, Rich) of the dry weight, respectively. The dried powder was dissolved in 1% (w / v) of 50% 1,3-butylene glycol solution and used in the present invention.

Production Example 3-21. Preparation of Lambutan and Rich Extract III

50.0 g of each of lambutan and rich fruit washed and washed with purified water was added to 1.2 L of the extraction solvent shown in Table 1, and the active ingredient was extracted at 4-40 ° C for 5 days. Then, the resultant was filtered with 300 mesh filter plugs, Left to stand for 10 days, and then aged, followed by filtration through Wattman # 5 filter paper. The filtrate was dried at 65 DEG C with a rotary attenuator to obtain a dry weight as shown in Table 1 below. The dried powder of each sample was dissolved in 1% (w / v) of 50% 1,3-butylene glycol solution and used in the present invention.

Extraction solvent Production Example No. /
Lambutan dry weight (unit: g) Manufacturing Example No.
/ Rich Dry Weight (Unit: g) water Production Example 3-1 / 12.5 Production Example 3-2 / 14.2 10% ethanol Production Example 4-1 / 15.9 Production Example 4-2 / 14.2 20% ethanol Production Example 5-1 / 16.8 Production Example 5-2 / 15.3 30% ethanol Production Example 6-1 / 13.7 Production Example 6-2 / 15.2 40% ethanol Production Example 7-1 / 15.7 Production Example 7-2 / 15.2 50% ethanol Production Example 8-1 / 16.7 Production Example 8-2 / 16.0 60% ethanol Production Example 9-1 / 16.5 Production Example 9-2 / 15.9 70% ethanol Production Example 10-1 / 16.8 Production Example 10-2 / 15.8 80% ethanol Production Example 11-1 / 14.3 Production Example 11-2 / 14.3 90% ethanol Production Example 12-1 / 15.1 Production Example 12-2 / 13.7 100% ethanol Production Example 13-1 / 15.5 Production Example 13-2 / 10.8 Methanol Production Example 14-1 / 15.4 Production Example 14-2 / 10.3 n-propanol Production Example 15-1 / 11.5 Production Example 15-2 / 9.8 Isopropanol Production Example 16-1 / 13.6 Production Example 16-2 / 12.6 2-butanol Production Example 17-1 / 11.3 Production Example 17-2 / 11.2 Acetone Production Example 18-1 / 12.5 Production Example 18-2 / 10.9 chloroform Production Example 19-1 / 12.4 Production Example 19-2 / 11.6 Ethyl acetate Production Example 20-1 / 11.5 Production Example 20-2 / 11.5 Butyl acetate Production Example 21-1 / 11.6 Production Example 21-2 / 11.5

Preparation Example 22. Preparation of lambutan and rich extract IV

Butylenglycol was dissolved in 1.2 L of 1,3-butylene glycol, extracted with active ingredient for 48 hours, filtered with 300 mesh filter cloth, left at 5-10 ℃ for 7-10 days After aging, the mixture was filtered through filter paper No. 5 of Wattsman. The extract was again dried to prepare a final concentration of 1% (w / v). The prepared rambutane extract and the rich extract were designated as Preparation Example 22-1 and Preparation Example 22-2, respectively.

Experimental Example 1. Inhibitory Effect of Lambutan and Rich Extract on MMP-1 Production

Fibroblasts (Korean Cell Line Bank, Korea), human normal skin cells, were inoculated into 48-well microplates (Nunc, Denmark) at a concentration of 1 10 ⁶ cells per well and cultured in DMEM medium (Sigma, For 24 hours, and then incubated with serum-free DMEM medium supplemented with the rambutane and rich extract prepared in Preparation Examples 1 to 22 to a final concentration of 100 占 퐂 / ml, and serum-free DMEM medium containing no rambutane and rich extract Followed by further incubation for 48 hours. After the incubation, the supernatant of each well was collected and the amount (ng / ml) of the newly synthesized MMP-1 was measured using an MMP-1 assay kit (Amersham, USA) The inhibition rate was calculated and the results are shown in Table 2. At this time, TGF-beta (10 ng / ml, Roche, USA) was used as a positive control for inhibition of MMP-1 production.

Inhibition rate of MMP-1 production by rambutane and rich extract (%) sample Inhibition rate of MMP-1 production (%) sample Inhibition rate of MMP-1 production (%) The positive control (TGF-β) 68.5 - - Production Example 1-1 62.5 Production Example 1-2 63.2 Production Example 2-1 68.3 Production example 2-2 62.5 Production example 3-1 64.3 Production example 3-2 63.3 Production Example 4-1 68.2 Production example 4-2 62.6 Production Example 5-1 65.5 Production Example 5-2 65.2 Production example 6-1 62.5 Production Example 6-2 62.3 Production Example 7-1 62.6 Production Example 7-2 65.6 Production Example 8-1 64.9 Production Example 8-2 66.5 Production Example 9-1 63.3 Production Example 9-2 64.3 Production Example 10-1 60.5 Production example 10-2 65.5 Production Example 11-1 64.8 Production example 11-2 64.2 Production Example 12-1 65.8 Production Example 12-2 66.3 Production Example 13-1 64.6 Production Example 13-2 62.5 Production Example 14-1 62.2 Production Example 14-2 63.8 Production Example 15-1 63.1 Production Example 15-2 66.5 Production Example 16-1 62.3 Production Example 16-2 65.5 Production Example 17-1 63.2 Production Example 17-2 62.5 Production example 18-1 62.5 Production Example 18-2 68.3 Production Example 19-1 62.6 Production Example 19-2 64.3 Production example 20-1 65.2 Production Example 20-2 68.2 Production Example 21-1 61.3 Production Example 21-2 65.5 Production Example 22-1 65.3 Production Example 22-2 62.5

As shown in the results of Table 2 above, it was confirmed that the inhibitory effect of the rambutane and the rich extract of the present invention on MMP-1 production was almost similar when compared with the positive control group TGF-β.

Experimental Example 2 Inhibitory Effect of Lambutane and Rich Extract on UV-induced MMP-1 Production

In general, it is known that the amount of MMP-1 is increased by ultraviolet light. Therefore, the present inventors conducted experiments to determine whether the level of MMP-1, which is produced by UV light, is inhibited by the rambutane and rich extract of the present invention. The experiment was carried out in the same manner as in Experimental Example 1, except that a certain amount of UVA was treated prior to the sample treatment. The percent inhibition of MMP-1 production was calculated according to Equation (1) above, and the results are shown in Table 3 below.

Inhibition rate of MMP-1 production by rambutane and rich extract after UV induction (%) sample Inhibition rate of MMP-1 production (%) sample Inhibition rate of MMP-1 production (%) The positive control (TGF-β) 78.5 - - Production Example 1-1 70.3 Production Example 1-2 71.3 Production Example 2-1 71.2 Production example 2-2 72.2 Production example 3-1 70.3 Production example 3-2 73.3 Production Example 4-1 75.1 Production example 4-2 72.1 Production Example 5-1 70.0 Production Example 5-2 71.0 Production example 6-1 70.6 Production Example 6-2 69.6 Production Example 7-1 69.8 Production Example 7-2 66.8 Production Example 8-1 74.5 Production Example 8-2 68.5 Production Example 9-1 71.2 Production Example 9-2 65.2 Production Example 10-1 72.3 Production example 10-2 70.3 Production Example 11-1 70.6 Production example 11-2 66.6 Production Example 12-1 69.8 Production Example 12-2 67.8 Production Example 13-1 66.8 Production Example 13-2 67.8 Production Example 14-1 67.8 Production Example 14-2 66.8 Production Example 15-1 70.9 Production Example 15-2 71.9 Production Example 16-1 69.8 Production Example 16-2 69.8 Production Example 17-1 70.2 Production Example 17-2 69.2 Production example 18-1 71.3 Production Example 18-2 68.3 Production Example 19-1 68.9 Production Example 19-2 69.9 Production example 20-1 66.8 Production Example 20-2 68.8 Production Example 21-1 67.8 Production Example 21-2 66.8 Production Example 22-1 70.3 Production Example 22-2 67.3

As shown in the results of Table 3, it was confirmed that the inhibitory effect of the rambutane and the rich extract of the present invention on MMP-1 production after UV irradiation was almost similar to that of the positive control group TGF-β.

Experimental Example 3. Enhancement of Collagen Synthesis Effect of Lambutan and Rich Extracts

Human normal epithelial cells, fibroblasts, were inoculated on a 48-well microplate at a concentration of 1 x 10 ⁶ cells per well and cultured in DMEM medium for 24 hours. The lambutan and rich extracts prepared in Preparative Examples 1 to 22 were further cultured in serum-free DMEM medium supplemented with 100 μg / ml of final concentration and serum-free DMEM medium containing no rambutane and rich extract for 48 hours . After the incubation, the supernatant of each well was collected and the amount of procollagen type I C-peptide (PICP) was measured by using a collagen kit (Takara, Japan) and converted into ng / ml, and the amount of synthesized collagen was measured Respectively. As a positive control for this experiment, 10 ng / ml of TGF-β was used. The increase rate of collagen biosynthesis was calculated according to the following equation (2), and the results are shown in Table 4.

Enhancement of Collagen Synthesis Effect of Lambutan and Rich Extract sample Collagen biosynthesis rate (%) sample Collagen biosynthesis rate (%) The positive control (TGF-β) 78.5 - - Production Example 1-1 68.6 Production Example 1-2 67.1 Production Example 2-1 66.5 Production example 2-2 67.4 Production example 3-1 68.9 Production example 3-2 68.5 Production Example 4-1 70.3 Production example 4-2 67.9 Production Example 5-1 77.3 Production Example 5-2 71.4 Production example 6-1 75.8 Production Example 6-2 67.6 Production Example 7-1 76.8 Production Example 7-2 77.8 Production Example 8-1 77.2 Production Example 8-2 76.8 Production Example 9-1 73.5 Production Example 9-2 77.2 Production Example 10-1 71.5 Production example 10-2 73.5 Production Example 11-1 72.6 Production example 11-2 71.7 Production Example 12-1 69.8 Production Example 12-2 62.6 Production Example 13-1 70.6 Production Example 13-2 69.8 Production Example 14-1 73.3 Production Example 14-2 68.7 Production Example 15-1 72.4 Production Example 15-2 63.3 Production Example 16-1 71.2 Production Example 16-2 62.4 Production Example 17-1 72.3 Production Example 17-2 62.2 Production example 18-1 70.0 Production Example 18-2 62.5 Production Example 19-1 69.0 Production Example 19-2 60.0 Production example 20-1 70.7 Production Example 20-2 69.2 Production Example 21-1 71.2 Production Example 21-2 60.2 Production Example 22-1 70.3 Production Example 22-2 61.2

As shown in the results of Table 3, it was confirmed that the collagen synthesis enhancing effect of the rambutane and the rich extract of the present invention was almost similar when compared with the positive control group TGF-β.

Experimental Example 4: Free radical scavenging action

The free radical scavenging activity of the rambutane extract (Preparation Example 10-1) and the rich extract (Preparation Example 10-2) prepared in Preparation Example 10 was measured as follows. That is, each of the plant extracts was added to 2 ml of a solution of 100 μM 1,1-diphenyl-2-picryl hydrazyl (DPPH) ethanol in 1000, 500, 250 , 100 ug / ml, and reacted at room temperature for 30 minutes. Then, absorbance was measured at 520 nm. Vitamin C was used as a comparative group. The free radical scavenging activity was calculated by the following formula. The results are shown in Table 5 below.

Free Radical Scavenging Effect of Lambutan and Rich Extracts Concentration (ug / ml) Lambutan extract Rich extract Vitamin C 1000 90.2% 88.2% 91.2% 500 89.8% 87.8% 90.8% 250 88.8% 86.5% 90.1% 100 80.2% 84.2% 89.8%

Formulations Examples of formulations of cosmetic compositions containing a lambutan and a rich extract are specifically shown, but the composition of the present invention is not limited to these formulations.

The extract of Preparation Example 10-1 was used as the rambutane extract used in the following formulation examples, and the extract of Preparation Example 10-2 was used as the rich extract. However, the extracts of other preparations may be used.

Formulation Example 1. Softening Time

Formulation examples of the softening longevity containing the rambutane extract and the rich extract obtained by the method of Preparation Example 10 were prepared as shown in Table 6 according to a conventional method.

ingredient Content (unit:% by weight) Lambutan extract
Rich extract
glycerin
1,3-butylene glycol
PEG 1500
Allantoin
DL-Panthenol
Lee D. TEE-2NA
Benzophenone-9
Sodium hyaruronate
ethanol
Octidodeces-16
Polysorbate 20
Preservative, fragrance, pigment
Distilled water 2.0
2.0
5.0
3.0
1.0
0.1
0.3
0.02
0.04
5.0
10.0
0.2
0.2
a very small amount
Balance Sum 100

Formulation Example 2. Convergent lotion

The formulation examples of the convergent lotion containing the rambutane and the rich extract obtained by the method of Production Example 10 were prepared as shown in Table 7 according to a conventional method.

ingredient Content (unit:% by weight) Lambutan extract
Rich extract
glycerin
1,3-butylene glycol
Allantoin
DL-Panthenol
Lee D. TEE-2NA
Benzophenone-9
Sodium hyaruronate
ethanol
Polysorbate 20
Location of Hazel extract
Citric acid
Preservative, fragrance, pigment
Distilled water 1.0
1.0
2.0
2.0
0.2
0.2
0.02
0.04
3.0
15.0
0.3
2.0
a very small amount
a very small amount
Balance Sum 100

Formulation Example 3. Nourishing Lotion

Formulation examples of the nutritional lotion containing rambutane and rich extract obtained by the method of Preparation Example 10 were prepared as shown in Table 8 according to a conventional method.

ingredient Content (unit:% by weight) Lambutan extract
Rich extract
Glyceryl stearate SE
Stearyl alcohol
lanolin
Polysorbate 60
Sorbitan stearate
Hardened vegetable oil
Mineral oil
Squalane
Trioctanoin
Dimethicone
Tocopheryl acetate
Carboxyvinyl polymer
glycerin
1,3-butylene glycol
Sodium hyaruronate
Triethanolamine
Preservative, fragrance, pigment
Distilled water 1.5
1.5
1.5
1.5
1.5
1.3
0.5
1.0
5.0
3.0
2.0
0.8
0.5
0.12
5.0
3.0
5.0
0.12
a very small amount
Balance Sum 100

Formulation Example 4. Nourishing Cream

Formulation examples of the nutritional cream containing the rambutane and the rich extract obtained by the method of Preparation Example 10 were prepared as shown in Table 9 according to a conventional method.

ingredient Content (unit:% by weight) Lambutan extract
Rich extract
Pro-type glycerin monostearate
Cetearyl alcohol
Stearic acid
Wax
Polysorbate 60
Sorbitan stearate
Hardened vegetable oil
Squalane
Mineral oil
Trioctanoin
Dimethicone
Sodium magnesium silicate
glycerin
Betaine
Triethanolamine
Sodium hyaruronate
Preservative, fragrance, pigment
Distilled water 2.0
2.0
2.0
2.2
1.5
1.0
1.5
0.6
1.0
3.0
5.0
5.0
1.0
0.1
5.0
3.0
1.0
4.0
a very small amount
Balance Sum 100

Formulation Example 5. Massage Cream

The formulation examples of the massage cream containing the rambutane and the rich extract obtained by the method of Preparation Example 10 were prepared as shown in Table 10 according to a conventional method.

ingredient Content (unit:% by weight) Lambutan extract
Rich extract
Chin type glycerin monosuccinic acid
Stearyl alcohol
Stearic acid
Polysorbate 60
Sorbitan stearate
Isostearyl isoserate
Squalane
Mineral oil
Dimethicone
Hydroxyethyl cellulose
glycerin
Triethanolamine
Preservative, fragrance, pigment
Distilled water 2.0
2.0
1.5
1.5
1.0
1.5
0.6
5.0
5.0
35.0
0.5
0.12
6.0
0.7
a very small amount
Balance Sum 100

Formulation Example 6. Essence

The formulation examples of the lambutan and the extract containing the rich extract obtained by the method of Production Example 10 were prepared as shown in Table 11 according to a conventional method.

ingredient Content (unit:% by weight) Lambutan extract
Rich extract
glycerin
Betaine
PIZZY 1500
Allantoin
DL-Panthenol
Lee D. T.-2Na
Benzophenone-9
Hydroxyethyl cellulose
Sodium hyaruronate
Carboxyvinyl polymer
Triethanolamine
Octyldodecanol
Octyldodec-16
ethanol
Preservative, fragrance, pigment
Distilled water 2.5
2.5
10.0
5.0
2.0
0.1
0.3
0.02
0.04
0.1
8.0
0.2
0.18
0.3
0.4
6.0
a very small amount
Balance Sum 100

Formulation Example 7. Pack

The formulation examples of the pack containing the rambutane and the rich extract obtained by the method of Production Example 10 were prepared as shown in Table 12 according to a conventional method.

ingredient Content (unit:% by weight) Lambutan extract
Rich extract
Polyvinyl alcohol
Cellulose sword
glycerin
PIZZY 1500
Cyclostristin
DL - Panthenol
Allantoin
Monoammonium glycyrrhizin acid
Nicotinamide
ethanol
FYGEN 40 Hardened castor oil
Fragrance, coloring, preservative
Distilled water 2.0
2.0
15.0
0.15
3.0
2.0
0.15
0.4
0.1
0.3
0.5
6.0
0.3
a very small amount
Balance Sum 100

EXPERIMENTAL EXAMPLE 4. Evaluation of Wrinkle-improving Effect of Cosmetics Containing Lambutan and Rich Extract

The wrinkle-improving effect of the cosmetic of the present invention was evaluated through practical use tests. The nutritional cream of Formulation Example 4 containing 2% (v / v) of the lamubook and the rich extract of the present invention, and the cream obtained by replacing the lamba-botan and the rich extract with purified water in Formulation Example 4 were evaluated. 30 out of 30 women aged 30-40 years were randomly divided into two groups, and the creams of Formulation Example 4 and Comparative Example were washed twice each morning and evening, and then the appropriate amount was continuously applied for 2 months around the eye area. The wrinkle improvement effect of each subject was evaluated by visual observation of a skilled physician. The experimental results are shown in Table 13 below.

Effect of wrinkle improvement (visual evaluation) Wrinkle improvement effect Effective rate (%) Great slightly none Cream of Formulation Example 4 24 4 2 85.0 Comparative cream 3 4 23 25.0

According to the experimental results, the inventive product containing the rambutane and the rich extract showed a higher wrinkle-reducing effect than the comparative example. In addition, skin irritation could not be observed on the skin of the subjects who applied the cosmetic composition of the present invention to the skin.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (8)

A cosmetic composition for preventing skin aging which contains as an active ingredient rambutane and a rich extract. A cosmetic composition for improving the wrinkles of skin, comprising as an active ingredient rambutane and a rich extract. 3. The method of claim 1 or claim 2, wherein the rambutane and rich extract are selected from the group consisting of water, anhydrous or lower alcohols having 1-4 carbons, acetone, ethyl acetate, chloroform, butyl acetate and 1,3-butylene glycol Wherein the extract is an extract obtained by using a solvent to be selected. The cosmetic composition according to any one of claims 1 to 3, wherein the rambutane and the rich extract are contained in an amount of 0.00001-30.0% (w / w) based on the total weight of the composition of the cosmetic composition. 3. The cosmetic composition according to claim 1 or 2, wherein the ratio of the content of the lampobut and the rich extract contained in the cosmetic composition is 1: 3 to 3: 1. The method of claim 1 or 2, wherein the rambutane and rich extract are selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, Wax foundation and spray. ≪ RTI ID = 0.0 > 11. < / RTI > A cosmetic method comprising applying the cosmetic composition according to any one of claims 1 to 2 to human skin to prevent skin aging and to improve skin wrinkles. A cosmetic method characterized by applying the cosmetic composition of claim 6 to human skin to prevent skin aging and to improve skin wrinkles.