KR101019085B1 - Filter container for extracting cells - Google Patents

Filter container for extracting cells Download PDF

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Publication number
KR101019085B1
KR101019085B1 KR1020100101642A KR20100101642A KR101019085B1 KR 101019085 B1 KR101019085 B1 KR 101019085B1 KR 1020100101642 A KR1020100101642 A KR 1020100101642A KR 20100101642 A KR20100101642 A KR 20100101642A KR 101019085 B1 KR101019085 B1 KR 101019085B1
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KR
South Korea
Prior art keywords
filter
container body
cells
lower container
cell
Prior art date
Application number
KR1020100101642A
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Korean (ko)
Inventor
김현창
Original Assignee
(주)패스텍
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Publication date
Application filed by (주)패스텍 filed Critical (주)패스텍
Priority to KR1020100101642A priority Critical patent/KR101019085B1/en
Application granted granted Critical
Publication of KR101019085B1 publication Critical patent/KR101019085B1/en
Priority to CN2011800118633A priority patent/CN102782493A/en
Priority to PCT/KR2011/003381 priority patent/WO2012053712A1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE: A filter container for collecting cells is provided to simply separate foreign materials from the cells and to easily collect the cells into a slide. CONSTITUTION: A filter container for collecting cells comprises: a container(110) having an open upper portion to contain a solution containing cells; a upper container body(100) having an insertion part and membrane member; a filter unit(210) which is detachably coupled into the insertion part and which is used for settling cells; and a lower container body(200) having a water discharge part. The filter unit comprises a settling groove having plural drain holes; a filter member(215) for collecting cell; and a support member for elastically support the filter member. The upper and lower container body further comprises a plurality of protrusions(140).

Description

Filter container for cell collection {FILTER CONTAINER FOR EXTRACTING CELLS}

The present invention relates to a filter container for cell collection, and in particular, the upper and lower containers are detachably coupled to each other, and having a membrane member and a filter member having different mesh sizes facilitate separation of cells contained in a preservation solution. The present invention relates to a filter container for cell collection, the structure of which is improved.

In general, to check for abnormalities in the body, cells corresponding to the physical examination site are collected, and the collected cells are preserved in a solution container containing a cell preservation solution, and the cells are attached to a slide and then examined under a microscope. Doing.

Conventionally, there is a separate solution container for dispersing the cells attached to the brush in the liquid, shake the solution container and pour the liquid into another container while the cells are sufficiently spread in the liquid and inhale the liquid by closely attaching the suction device under the filter. In addition, the filter is filtered by rubbing the cells filtered on the slide.

When the container and the body are separated to bring the sampling membrane to the slide, the mixed liquid left in the container is poured down by gravity and disappeared.

However, the process of moving the cells from the solution container to the slides is time-consuming because it must be done manually by the user, and contaminants or impurities can be transferred from the user's hand to the slides, thereby reducing the accuracy of the cell test. there was.

In addition, foreign matter may be contained in addition to the cells collected in the solution container, in which case it is not easy to separate the foreign matter and cells, a separate tool for separation is required.

SUMMARY OF THE INVENTION An object of the present invention is to provide a filter container for cell harvesting, in which the preservation solution can be easily separated from the collected cells, and the structure of the cell collection can be improved to easily collect the separated cells.

The present invention for achieving the above object is to distinguish between the receiving portion and the receiving portion, the upper portion is opened to accommodate the solution containing the cells, the insertion portion formed to be opened to the lower side and the lower portion of the receiving portion, An upper container body composed of a membrane member disposed so as to be disposed therein;

The filter unit is detachably coupled so that an upper portion is accommodated in the fitting portion, and the cell portion having passed through the membrane member is seated on an upper surface thereof, and a space disposed below the filter portion to drain a solution passing through the filter portion. And a lower container body composed of a drain portion.

The filter unit is formed in the upper surface of the lower container body and the recessed groove of the groove-type is formed with a plurality of drain holes,

A filter member disposed above the seating groove and separating the solution and the cells passing through the membrane member to collect the cells;

A support member is disposed between the filter member and the seating recess to elastically support the filter member upward.

The upper and lower container body further includes a stopper means for preventing slip rotation of the upper container body when engaged.

The stopper means is formed on the outer circumferential surface of the lower container body and the engaging projection of the form protruding outward,

And a locking groove formed on an inner circumferential surface of the fitting portion of the upper container body so as to fit with the locking protrusion.

The filter member and the membrane member are configured in a mesh structure, more preferably, the filter member is formed with a larger number of meshes than the membrane member.

The upper and lower container bodies further include a plurality of protrusions formed on each outer circumferential surface to be spaced apart from each other.

The present invention can easily separate the cells and foreign matter contained in the cell preservation solution and its structure is improved so that the separated cells can be easily collected, according to the present invention, the upper and lower parts are separated from each other and assembled The detachable coupling structure is possible, and the membrane member and the filter member can be used to easily separate the cells and foreign substances contained in the cell preservation solution, and have a useful effect of easily collecting the separated cells on the slide.

1 is an exploded perspective view of a filter container for cell collection according to the present invention.
Figure 2 is a perspective view showing a combined state of the present invention.
3 is an exploded perspective view showing the filter unit of the present invention.
Figure 4 is a state diagram showing the cell filter process of the present invention.
Figure 5 is a use state showing the state before collecting the separated cells of the present invention on the slide.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

When the filter container for cell collection according to the present invention will be described with reference to Figs. 1 to 5, its configuration is largely the upper container body 100 and the lower container detachably coupled to the lower portion of the upper container body 100. It is roughly divided into the main body 200.

In more detail, the upper container body 100 has a receiving portion 110 having an upper portion opened to accommodate a solution containing the cells C, and a fitting formed to be opened below the receiving portion 110 and opened downward. It is arranged to distinguish between the portion 120, the receiving portion 110 and the fitting portion 120 is composed of a membrane member 150 of the mesh structure for filtering the foreign matter contained in the solution.

That is, the upper container body 100 is formed in a shape in which the upper and lower receiving portions 110 and the fitting portion 120 are opened up and down, respectively, based on the membrane member 150 disposed horizontally in the middle. have.

The lower container body 200 is coupled to be accommodated in the fitting portion 120, the filter portion 210 and the filter portion 210 is seated on the upper surface of the cell (C) passing through the membrane member 150, Is disposed on the lower side is composed of a drain 220 having a space for draining the solution passing through the filter (210).

The filter unit 210 is formed on the upper surface of the lower container body 200 and is disposed in the groove-shaped seating groove 212 and the seating groove 212 formed with a plurality of drain holes 212a, the membrane member 150 The filter member 215 of the mesh structure for separating the solution (C) and the cell (C) passing through the) and the cell (C) to be collected, and disposed between the filter member 215 and the seating groove 212, the filter member (215) It is composed of a support member 216 for elastically supporting upward.

The support member 216 may support the filter member 215 upwards, and may employ a compressed sponge material to enable drainage of the cell preservation solution, but is not limited thereto.

The membrane member 150 and the filter member 215 each have a mesh structure having a plurality of holes, and preferably, the number of meshes of the filter member 215 is greater than the number of meshes of the membrane member 150. .

This is for the membrane member 150 to pass through the cells (C) contained in the cell preservation solution and to filter out foreign substances by blocking the passage of foreign matter (larger than the cells) contained in the cell preservation solution. In the filter member 215, the cells C passing through the membrane member 150 are seated on the upper side of the filter member 215, and only the solution is discharged to the outside through the lower drain hole 212a.

For example, the number of meshes of the membrane member 150 may be 150 to 300 mesh, and the number of meshes of the filter member 215 may be 500 mesh or more.

In addition, the upper and lower container body 100, 200 is provided with a stopper means for preventing slip rotation of the upper container body 100 at the time of coupling.

The stopper means is formed on the outer circumferential surface of the lower container body 200 and is provided on the inner circumferential surface of the fitting portion 120 of the upper container body 100 so as to be engaged with the locking protrusion 230 and the locking protrusion 230. It is provided with a locking groove 130 is formed.

In addition, the upper and lower container bodies 100 and 200 are formed with a plurality of protrusions 140 and 240 spaced apart from each other on each outer circumferential surface.

This is to facilitate gripping during the clamping operation with the clamping mechanism of the inspection device (not shown) which clamps the upper and lower container bodies 100 and 200 and collects the cells C, respectively.

That is, the clamping mechanism is for suppressing flow when the lifting operation is performed after clamping the outer peripheral surfaces of the upper and lower container bodies 100 and 200.

The operation of the present invention having such a configuration will now be described.

In the filter container for collecting cells according to the present invention, first, the assembling process of the upper container body 100 and the lower container body 200, the lower container body 200 in the fitting portion 120 of the upper container body 100; When the upper portion of the) is inserted, the filter unit 210 of the lower container body 200 is disposed below the membrane member 150 of the upper container body 100.

At this time, the locking protrusion 230 of the lower container body 200 is fitted into the locking groove 130 formed on the inner circumferential surface of the upper container body 100 to prevent slip rotation of the upper container body 100.

According to the present invention after the assembly process, the cell preservation solution of the solution container is poured on the side of the receiving part 110 having the upper opening, and the cells C contained in the cell preservation solution and the cell preservation solution pass through the membrane member 150. By entering the fitting portion 120, foreign matter contained in the cell preservation solution does not pass through the membrane member 150 is filtered.

Subsequently, the solution introduced into the fitting portion 120 passes through the filter member 215 and the supporting member 216 and flows into the draining portion 220 through the drain hole 212a and into the fitting portion 120. The introduced cells C are filtered to be seated on the upper side of the filter member 215.

Thereafter, the solution introduced into the drain 220 is discharged to the outside through a separate hose member connected to the drain 220.

After the slide S of the glass material is slid and moved to the upper side of the filter member 215, when the lower container body 200 is lifted up through the clamping mechanism, the cells attached to the upper side of the filter member 215 are removed. (C) is taken while being attached to the lower side of the slide (S).

At this time, the support member 216 elastically supports the filter member 215 upwards, thereby protecting the slide (S) when the filter member 215 and the slide (S) in contact with the adhesion of the cells (C). Perform the function to improve.

The upper vessel body 100 is lifted up by a separate clamping mechanism before the transfer operation of the slide S and is separated from the lower vessel body 200.

Thereafter, the cells (C) attached to the lower side of the slide (S) can be observed under a microscope to check the abnormality of the collected cells (C).

Therefore, the present invention has a removable coupling structure that can be separated and assembled to each other, the upper and lower parts, and can easily separate the cells and foreign matter contained in the cell preservation solution using the membrane member 150 and the filter member 215, The separated cells have an advantage that can be easily collected on the slide (S).

Explanation of symbols on the main parts of the drawings
100: upper container body 110: receiving portion
120: fitting portion 130: locking groove
140: projection part (upper container body)
150 membrane member 200 lower container body
210: filter part 212: mounting groove
212a: drain hole 215: filter member
216: support member 220: drain
230: locking projection 240: projection (of the lower container body)
C: Cell S: Slide

Claims (6)

An upper container body consisting of a receiving portion having an upper portion opened to accommodate a solution containing cells, a fitting portion disposed below the receiving portion and formed to open downward, and a membrane member disposed to distinguish between the receiving portion and the fitting portion. Wow;
The filter unit is detachably coupled so that an upper portion is accommodated in the fitting portion, and the cell portion having passed through the membrane member is seated on an upper surface thereof, and a space disposed below the filter portion to drain a solution passing through the filter portion. And a lower container body composed of a drainage portion. The method according to claim 1,
The filter unit is formed in the upper surface of the lower container body and the recessed groove of the groove-shaped recess formed with a plurality of drain holes,
A filter member disposed above the seating groove and separating the solution and the cells passing through the membrane member to collect the cells;
And a support member disposed between the filter member and the seating groove to elastically support the filter member upwardly. The method according to claim 1,
The upper and lower container body further comprises a stopper means for preventing slip rotation of the upper container body when coupled to the filter container for cell collection. The method according to claim 3,
The stopper means is formed on the outer circumferential surface of the lower container body and the engaging projection of the form protruding outward,
Cell collecting filter container characterized in that it has a locking groove formed on the inner peripheral surface of the fitting portion of the upper container body to be fitted with the locking projection. The method according to claim 2,
The filter member and the membrane member is composed of a mesh structure,
The filter member is a cell collection filter container, characterized in that the number of mesh more than the membrane member. The method according to any one of claims 1 to 5,
The upper and lower container body is a filter container for cell collection, characterized in that it further comprises a plurality of protrusions formed on each outer peripheral surface spaced apart from each other.
KR1020100101642A 2010-10-19 2010-10-19 Filter container for extracting cells KR101019085B1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
KR1020100101642A KR101019085B1 (en) 2010-10-19 2010-10-19 Filter container for extracting cells
CN2011800118633A CN102782493A (en) 2010-10-19 2011-05-06 Filter container for collecting cells
PCT/KR2011/003381 WO2012053712A1 (en) 2010-10-19 2011-05-06 Filter container for collecting cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020100101642A KR101019085B1 (en) 2010-10-19 2010-10-19 Filter container for extracting cells

Publications (1)

Publication Number Publication Date
KR101019085B1 true KR101019085B1 (en) 2011-03-07

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KR1020100101642A KR101019085B1 (en) 2010-10-19 2010-10-19 Filter container for extracting cells

Country Status (3)

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KR (1) KR101019085B1 (en)
CN (1) CN102782493A (en)
WO (1) WO2012053712A1 (en)

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KR101348779B1 (en) 2012-03-27 2014-01-07 설규남 Membrane filter structure and cytology smear method using the same
KR20150145744A (en) * 2014-06-19 2015-12-31 (주)아모레퍼시픽 Container and method for measuring for strength of bead
KR20160061645A (en) 2014-11-24 2016-06-01 (주)패스텍 Membrane filter container for cytology smear device
KR20160118093A (en) * 2015-04-01 2016-10-11 (주)한국티씨엠 A filter for preparing liquid-based cytology specimen slide
WO2016167515A1 (en) * 2015-04-14 2016-10-20 (주)셀트라존 Liquid specimen smear processing device
KR101694422B1 (en) 2016-06-28 2017-01-10 (주) 굿모닝 바이오 Filtration vessel for adipose stem cells
WO2017099357A1 (en) * 2015-12-10 2017-06-15 주식회사 인셀바이오 Sample extraction container assembly including pipette
KR101758352B1 (en) * 2014-11-24 2017-07-31 (주)패스텍 Smear device of cytology for making microscope slides and the smearing method thereof
KR101838220B1 (en) * 2017-05-22 2018-03-13 (주) 굿모닝 바이오 Container and method for extracting adipose stem cells
WO2018225884A1 (en) * 2017-06-08 2018-12-13 (주)알엠바이오 Sample collection container assembly comprising pipette
WO2020050504A1 (en) * 2018-09-08 2020-03-12 (주)바이오다인 Vial device for storing and smearing exfoliative cell
WO2020082418A1 (en) * 2018-10-24 2020-04-30 清华大学 Plankton settling device for preventing air bubble interference
KR20200125387A (en) * 2019-04-26 2020-11-04 가부시끼가이샤 옵토니쿠스 세이미쯔 Filter device, and manufacturing method for slide glass specimen
KR20220063425A (en) * 2020-11-10 2022-05-17 한국지질자원연구원 Portable water filtration device

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KR20150145744A (en) * 2014-06-19 2015-12-31 (주)아모레퍼시픽 Container and method for measuring for strength of bead
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KR20160061645A (en) 2014-11-24 2016-06-01 (주)패스텍 Membrane filter container for cytology smear device
KR101758352B1 (en) * 2014-11-24 2017-07-31 (주)패스텍 Smear device of cytology for making microscope slides and the smearing method thereof
KR20160118093A (en) * 2015-04-01 2016-10-11 (주)한국티씨엠 A filter for preparing liquid-based cytology specimen slide
KR101693972B1 (en) * 2015-04-01 2017-01-06 (주)한국티씨엠 A filter for preparing liquid-based cytology specimen slide
WO2016167515A1 (en) * 2015-04-14 2016-10-20 (주)셀트라존 Liquid specimen smear processing device
WO2017099357A1 (en) * 2015-12-10 2017-06-15 주식회사 인셀바이오 Sample extraction container assembly including pipette
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WO2018225884A1 (en) * 2017-06-08 2018-12-13 (주)알엠바이오 Sample collection container assembly comprising pipette
JP7099773B2 (en) 2018-09-08 2022-07-12 バイオダイン カンパニー リミテッド Vial device for storage and smearing of exfoliated cells
WO2020050504A1 (en) * 2018-09-08 2020-03-12 (주)바이오다인 Vial device for storing and smearing exfoliative cell
KR20200029111A (en) * 2018-09-08 2020-03-18 (주)바이오다인 vial device of preparing storing and smearing of exfoliative cells
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US11951473B2 (en) 2018-09-08 2024-04-09 Biodyne Co., Ltd. Vial device for storing and smearing exfoliative cells
JP2021536009A (en) * 2018-09-08 2021-12-23 バイオダイン カンパニー リミテッドBiodyne Co., Ltd. Vial device for storage and smearing of exfoliated cells
WO2020082418A1 (en) * 2018-10-24 2020-04-30 清华大学 Plankton settling device for preventing air bubble interference
KR20200125387A (en) * 2019-04-26 2020-11-04 가부시끼가이샤 옵토니쿠스 세이미쯔 Filter device, and manufacturing method for slide glass specimen
KR102255785B1 (en) * 2019-04-26 2021-05-24 가부시끼가이샤 옵토니쿠스 세이미쯔 Filter device, and manufacturing method for slide glass specimen
KR102399274B1 (en) * 2020-11-10 2022-05-18 한국지질자원연구원 Portable water filtration device
KR20220063425A (en) * 2020-11-10 2022-05-17 한국지질자원연구원 Portable water filtration device

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Publication number Publication date
CN102782493A (en) 2012-11-14
WO2012053712A1 (en) 2012-04-26

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