KR101013456B1 - Composition for the culturing of simplicillium lamellicola bcp and culturing method using the composition - Google Patents

Abstract

PURPOSE: A medium composition for culturing Simplicillium lamellicola BCP and a culture method are provided to enhance spore production and to improve spore stability. CONSTITUTION: A medium composition for culturing Simplicillium lamellicola BCP contains 1.0-5.0 w/v% of carbon source of fructose, dextrin, or sucrose and 0.5-4.0 w/v% of nitrogen source of soybean meal or tryptone. The composition additionally contains an inorganic source of magnesiuim sulfate, potassium chloride, magnesium chloride, calcium chloride, sulfate zinc, potassium nitrate, ammonium nitrate, monobasic potassium phosphate, iodination potassium or sodium molybdate.

Description

Composition for the culturing of Simplicillium lamellicola BCP and culturing method using the composition}

The present invention relates to a medium composition for culturing Simpliclium lamella cola BCP strains and a method of culturing the same.

Mycelial growth or mycelial shape may vary depending on which medium is used for strain culture. When culturing strains, the strains may show changes in mycelial growth or irregular changes in the tip of the flora, which are often caused by changes in the composition or culture conditions of the medium. In some cases, depending on the type of medium, there may be difficulty in mass propagation of the bacteria.

Therefore, it is important to select a medium that can replace the existing PDB (potato dextrose broth) while optimizing the culture of Simpliclium lamelicola BCP strains. PDB has been mainly used for the cultivation of S. simillium lamelicola BCP strains, but PDB is a complex medium has a problem that does not know the exact components.

The present invention provides an optimal medium composition for the cultivation of Simpliclium lamella cola BCP strains and a method of culturing the S. similisium lamellicola BCP strain using the same, and specifically the present invention is a group consisting of sucrose, fructose and dextrin At least one carbon source selected from; And soybean meal (soybean meal) and florisil simple Solarium la mellitic coke BCP (Simplicillium containing one or more nitrogen sources selected from the group consisting of Tryptone (tryptone) with medium components It is an object of the present invention to provide a medium composition for lamellicola BCP) strain culture and a method for culturing Simpliclium lamella cola BCP using the composition.

In order to solve the above problems, the present invention provides a medium composition comprising an optimal carbon source and a nitrogen source, and by setting the optimum incubation temperature, initial culture pH, incubation time, the culture medium composition for simplicity lamella cola BCP strain culture and Culture conditions were optimized.

The present invention can obtain a homogeneous medium by establishing the optimum medium composition and culture conditions of the Simpliclium lamellicola BCP strain, can increase the yield of spores more than twice the Simpliclium lamellicola BCP strain, This has the advantage of greatly improving spore stability .

Figure 1 shows the results of measuring the spores production yield of Simplisium lameticola BCP according to the culture temperature.
Figure 2 shows the results of measuring the spores production yield of Simplisium lame- licola BCP according to the initial pH of the culture.
Figure 3 is a graph of the spore production of the Simplisium lamelicola BCP over time incubation.
Figure 4 is a graph of spore production of Simplisium lamelicola BCP according to the initial inoculation concentration.
Figure 5 shows the results of measuring the spores production yield of Simplisium lamelicola BCP according to fructose concentration.
Figure 6 shows the results of measuring the spores production yield of Simpliclium lamellicola BCP according to the soybean meal concentration.
Figure 7 shows the results of the main effect analysis on the spores production yield of Simpliclium lamelicola BCP strains according to Plackett-Burman Design (PBD).
Figure 8 is a graph showing the change in culture yield of Simplisium lamelicola BCP according to the type of medium.
Figure 9 is a graph showing the changes over time stability of Simplisium lamelicola BCP according to the type of medium.

The present invention relates to an optimal medium composition for the cultivation of Simpliclium lamella cola BCP strains and a method of culturing the S. similisium lamelicola BCP strain using the same.

Hereinafter, the present invention will be described in detail.

The present invention provides at least one carbon source selected from the group consisting of sucrose, fructose and dextrin; And soybean meal (soybean meal) and florisil simple Solarium la mellitic coke BCP (Simplicillium containing one or more nitrogen sources selected from the group consisting of Tryptone (tryptone) with medium components lamellicola BCP) strain culture medium composition.

In the medium composition of the present invention, the carbon source may contain 1.0 to 5.0 w / v%, preferably 2 to 4 w / v%, and more preferably 3 to 3.5 w / v%. Outside the above range, the yield of spore production may be lowered.

The medium composition of the present invention may contain 0.5 to 4.0 w / v%, preferably 2 to 4.0 w / v%, more preferably 2.5 to 3.0 w / v%. Outside the above range, the yield of spore production may be lowered.

The medium composition of the present invention may further include an inorganic source. In one embodiment of the invention, but not limited to, magnesium sulfate, potassium chloride, magnesium chloride, sodium chloride, ferrous sulfate, calcium chloride, dipotassium phosphate, copper sulfate, zinc sulfate, potassium nitrate, ammonium nitrate At least one selected from the group consisting of potassium dihydrogen phosphate, potassium iodide and sodium molybdate is preferred.

In particular, the present invention provides a medium composition for the culture of Simpliclium lamella cola BCP strain comprising fructose, soy flour, and magnesium sulfate as a medium component.

The medium is more preferably 1.0 to 5.0 w / v% fructose; Soy flour of 0.5 to 4.0 w / v%; By including 0.1 to 1 w / v% magnesium sulfate, it is possible to increase the yield of spores of the Simpliclium lamellicola BCP strain.

In addition, the medium may further include an inorganic source such as potassium chloride, potassium diphosphate and iron sulfate, and the medium includes 0.1 to 1 w / v% of potassium chloride; 0.05-0.5 w / v% potassium diphosphate; And 0.0001 to 0.01 w / v% of iron sulfate.

The present invention provides a method of culturing the Simpliclium lamella cola BCP strain in the above-mentioned medium composition.

The medium prepared according to the medium composition is inoculated with a Simlyllium lamelicola BCP strain seed having an initial concentration of 0.1 to 5 w / v%, more specifically 0.5 to 2 w / v%. Outside the concentration range, there is a fear that growth is somewhat lower.

In the culture, the culture temperature may be 15 to 37 ° C, preferably 15 to 33 ° C, more preferably 20 to 25 ° C. If the culture temperature is 15 ℃ or less, the growth rate is increased, but the growth rate is slow and the spore formation yield may be greatly reduced, if it is above 37 ℃ contamination occurs easily and the amount of the final culture product may be reduced.

In culture, the pH is suitably adjusted to 3 to 8, preferably pH 3.5 to 6, more preferably pH 4 to 5. If the pH is less than 3, it is almost impossible to grow, there is a fear that the yield of spore production is low, and if the pH is exceeded 8, the amount of the final culture may decrease. The pH refers to the pH of the initial culture, the initial culture refers to the sterilization of the nutrient medium and immediately after the inoculation of the bacteria in the state before the inoculation of the simple bacilli. The pH adjustment is made before sterilization, but the final pH measurement is made immediately after sterilization, so the pH at the time of inoculation and growth of the first bacteria is taken as the initial pH of the culture.

In the culture, the culture period may be 20 hours or more, specifically 30 hours to 7 days, more specifically 50 hours to 6 days on the basis of 500 to 5000 L incubator. If the incubation period is less than 20 hours, there is a fear that the yield is low when the drying period is more than the spores, and if the incubation period exceeds 7 days, the material produced during the culture process may kill the microorganisms and the yield may be lowered.

In the culture, the stirring speed and the air input rate may be appropriately set according to the growth of the microorganism, and may vary depending on the culture conditions and conditions. In addition, since bubbles generated during the culture may cause problems such as contamination, it is preferable to remove them. For example, defoaming may be performed by using a device such as KONION GE-304. The initial input ratio may be 0.1 to 1 w / v% level, it can be injected into the vesicles every time bubbles are generated at the level of 0.01 to 0.1 w / v% during the culture.

Immediately after the initial culture, the freeze-drying may be performed by freezing at a temperature of -90 to -10 ° C, specifically -80 to -20 ° C, more specifically -70 to -30 ° C, but freezing at a time due to the large amount of the culture solution. If it is difficult to reduce the contamination, the final pH can be adjusted to less than 5 to suppress contamination and propagation of microorganisms.

Hereinafter, the present invention will be described in more detail through examples according to the present invention and comparative examples not according to the present invention, but the scope of the present invention is not limited to the examples given below.

< Example  1>

Growth medium was prepared according to the composition described in Table 1 below:

Badge composition Composition Content (w / v%) Input amount (g / L) Fructose 3.0 30 Soybean meal 3.0 30 MgSO ₄ ㆍ 7H ₂ O 0.5 5 KCl 0.5 5 K ₂ HPO ₄ 0.1 One FeSO ₄ 7H ₂ O 0.001 0.01

< Experimental Example >

1. Selection of strains and basal medium

Simplicium lamelicola BCP strain was used by the Korea Research Institute of Chemical Technology [Accession Number: KCTC0639BP]. Inoculating the strain 1 w / v% in the medium was carried out the following experiment.

CDB (Czapek-Dox Broth: sucrose 30 g / L, sodium nitrate 3.0 g / L, dipotassium phosphate 1.0 g / L, magnesium sulfuate 0.5 g / L, potassium chloride 0.5 g / L, ferrous sulfate 0.01 g / L , pH 7.3).

2. Review physical and chemical culture conditions

The effects of physical and chemical culture conditions such as culture temperature, initial pH and culture time on CDB medium (50ml, 250ml-triangular flasks) on the spore production of S. aureum B. sp.

Five 5 × 5 mm sections of the flora grown on PDA for 7-10 days were inoculated. To examine the yield of spore production, the number of spores in the culture was counted using a homocytometer under a 400x microscope. The physical properties of the cultures in the culture were generally homogeneous, but a large number of small cell masses were formed as a whole, and microscopic observations showed that many spores were collected in the mass. Thus, after incubation, the homogenizer was crushed for 10 seconds to homogenize the spores, and then suspended in a vortex mixer, and the number of spores was counted. The test was repeated three times.

(1) measuring the effect of incubation temperature

Initial pH was 7.3 when the medium was prepared, the incubation temperature was changed to 20, 22, 25, 27, 30 ℃ for 5 days (120 rpm), and then the number of spores in the culture was counted according to the method described above.

Simplisium lamelicola BCP showed a slight drop in sporulation yield at a low temperature of 20 ° C. or lower, 22 ° C. at an optimum temperature, and a decrease in sporulation yield since 25 ° C. [FIG. 1].

(2) initial culture pH The impact of

The culture temperature was fixed at 22 ℃, the pH of the medium was adjusted to 4, 5, 6, 7, 8 using HCl and NaOH for 5 days incubation (120 rpm), and the number of spores in the culture was counted.

As a result of examining the effect of the initial pH of the medium on the spore production yield of the Simlyillium lamelicola BCP, the spore production yield was low at the basic pH of 7.0 or higher, excellent at pH 4.0 ~ 5.0, and lower than the 6.0. The optimum pH was determined to be 4.5 [FIG. 2].

(3) Effect of incubation time

Samples were taken at 5 ml intervals at 24 hour intervals during a 9-day incubation (150 rpm) for 3 days in a 1 L flask (working volume 200 ml) at an optimum temperature of 22 ° C. and an optimum initial pH of 4.5 determined in the two experiments. Spore count was counted.

As a result of investigating the trend of spore production according to the incubation time, almost no spores were formed until the 3rd day and rapidly formed after the 3rd day and reached almost the maximum level on the 5th day, and remained constant until the 8th day (FIG. 3). .

3. Experiment of medium optimization condition

(One) On carbon  Impact measurement

In order to investigate the effect of spore production yield on the carbon source, sucrose, which is used as the carbon source of CDB medium, was prepared by substituting 1.5% (w / v) of different carbon sources. Carbon sources tested in this experiment were soluble starch, dextrin, maltose, lactose, mannitol, galactose, fructose, glucose, and CMC. It was. Culture conditions were fixed at a temperature of 22 ℃, initial pH 4.5, 120 rpm and incubated for 5 days. The number of spores in the final culture was counted and the physical properties of the culture were also investigated. The test was repeated three times.

As a result of investigating the effect of carbon source on the spore production yield, the carbon source showed better effect than sucrose of CDB, the basal medium, and fructose was lower than other sucrose. 2). Soluble starch had a very large mycelial mass, and maltose, lactose and galactose also had a relatively large mycelial mass, while the spore count was low. Glucose had a relatively low mycelial mass but low spore count. Dextrin was very homogenous with little lumps in culture, but had a relatively low spore count. Fructose was less homogeneous than basal medium CDB, but spore count increased about 47% and little mycelial mass was seen. Mold growth did not occur in mannitol and CMC. This was thought to be due to the fact that the carbon source of this type was not available to the Simlyillium lamelicola BCP.

Effects of Carbon Sources on Spore Production Yield and Culture Characteristics of Simpliclium Lamelicola BCP Carbon source
(1.5 w / v%) Spore Density
(conidia / ml) Characteristics of culture Soluble starch 3.2E + 6 Mycelial mass formation (+++) Dextrin 4.2E + 7 Homogeneous (+++) Maltose 6.3E + 6 Mycelial mass formation (++) Lactose 1.9E + 7 Mycelial mass formation (++) Manitol Not growing - Galactose 7.5E + 6 Mycelial mass formation (++) Fructose 2.7E + 8 Homogeneous (+) Glucose 3.4E + 6 Mycelial mass formation (+) CMC Not growing - Sucrose (Czapek-Dox broth, contrast) 1.7E + 8 Homogeneous (++)

(2) fructose ( Fructose ) Effect of concentration

In the carbon source experiment, the yield of spore production according to the concentration was investigated for fructose, which had the highest yield of spore production and excellent physical properties of the culture solution. The medium was prepared by removing the sucrose used as a carbon source from the CDB basal medium and replacing the fructose with an increase in the fructose content from 1.0 w / v% to 5.0 w / v% in units of 1.0 w / v%. Culture conditions were fixed at a temperature of 22 ℃, initial pH 4.5, 120 rpm and incubated for 5 days. Spore count was counted in the final culture. The test was repeated three times.

The optimum concentration of fructose was investigated according to the above carbon source test results. Testing from 1.0 w / v% to 5.0 w / v% at intervals of 1.0 w / v% showed high yield of spore production from 3.0 to 5.0 w / v% and decreased before that (Figure 5). . Considering the variation in carbon source concentration of fructose, the optimum condition was set to 3.0%.

(3) measuring the effect of nitrogen source

In order to investigate the effect of nitrogen source spore production yield, NaNO ₃ used as a nitrogen source of CDB medium was removed by removing 3 w / v% and adding 1.0 w / v% of each other nitrogen source. The carbon source replaced 3 w / v% sucrose with 3 w / v% fructose determined in the previous experiment. The nitrogen sources tested in this experiment were soybean meal, yeast extract, tryptone, peptone, beep extract, (NH ₄ ) ₂ SO ₄ , NH ₄ Cl, NH ₂ CO ₂ , NH ₃ NO ₃ . Culture conditions were fixed at a temperature of 22 ℃, initial pH 4.5, 120 rpm and incubated for 5 days. Spore count was counted in the final culture.

As a result of investigating the effect of nitrogen source on spore production yield, soybean meal and tryptone were shown to be superior to sodium nitrate in basal medium CDB (Table 3). Overall, the nitrogen source in the form of ammonia was found to be poorly used by the Simlyillium lamelicola BCP.

Influence of Nitrogen Sources on Spore Production Yield and Culture Characteristics of Simplisium Lamelicola BCP Nitrogen source
(1.0 w / v%) Spore Density
(conidia / ml) Soybean meal 5.0E + 8 Yeast extract 2.8E + 8 Tryptone 1.4E + 8 Peptone 7.5E + 7 Beef extract 1.2E + 6 (NH ₄ ) ₂ SO ₄ 1.3E + 4 NH ₄ Cl <1.0E + 4 (below detection limit) NH ₂ CO ₂ <1.0E + 4 (below detection limit) NH ₃ NO ₃ 1.2E + 7 NaNO ₃ (Czapek-Dox broth, control) 1.2E + 8

(4) soybean meal ( Soybean meal ) Effect of concentration

In the nitrogen source experiment, the yield of spore production according to the concentration was investigated for the soybean meal having the highest yield of spores and excellent physical properties of the culture solution. In the basal medium CDB, the carbon source was 3 w / v% of fructose and the nitrogen source was prepared by increasing the soy flour by 1.0 w / v% from 0.5 w / v% to 4.0 w / v%. Culture conditions were fixed at a temperature of 22 ℃, initial pH 4.5, 120 rpm and incubated for 5 days. Spore count was counted in the final culture. The test was repeated three times.

In order to determine the optimal concentration of soy flour selected as an excellent nitrogen source in the above nitrogen source experiment, the concentration test was performed from 0.5 w / v% to 4.0 w / v% in units of 1.0 w / v% from 3.0 to 4.0 w / v%. Showed the maximum spore production yield (Fig. 6). However, below 2.0 w / v%, the yield of spores was rapidly decreased. Considering the variation of nitrogen source concentration of soy flour and raw material cost, the optimum condition was set to 3.0 w / v%.

(5) In mineral garden  Effect

Nine minerals (magnesium sulfate, sodium chloride, potassium chloride, potassium chloride, potassium sulfate, potassium sulfate, ferrous sulfate) except potassium diphosphassium (phosphate), magnesium sulfate (potassium chloride), and ferrous sulfate in the CDB Ferrous sulfate, calcium chloride, cupric sulfate, magnesium chloride, Zinc sulfate, barium chloride) were added to the yield of spores by adding 0.1 w / v%. A carbon source of 3.0 w / v% of fructose and a nitrogen source of 3.0 w / v% were added. Culture conditions were incubated for 5 days at 25 ℃, initial pH 4.5, 120 rpm. Spore count in the final culture was counted and the test was repeated three times.

As a result of investigating the effect of various mineral sources on the yield of spores, there was no effect of increasing the yield of spores from a single mineral (Table 4). This may mean that several minerals need a certain amount at the same time, or that neither is absolutely necessary.

Influence of Mineral Sources on Spore Production Yield and Culture Characteristics of Simplisium Lamelicola BCP Mineral garden
(0.1 w / v%) Spore Density
(conidia / ml) Magnesium Sulfate 1.0E + 8 Sodium chloride 2.3E + 7 Potassium chloride 1.8E + 8 Ferrous sulfate 1.6E + 7 Calcium chloride 8.2E + 7 Cupric sulfate 5.6E + 7 Magnesium chloride 1.2E + 8 Zinc sulfate 5.6E + 7 Barium chloride <1.0E + 4

Because it is difficult to determine the mineral source required by this experiment, we tried to select the mineral source that is needed mainly by applying the PBD technology that finds the main effect centering on the mineral source that is mainly used in CDB.

(6) PBD ( Plackett - Burman Design )

In the above experiment, the yield of spores was greatly increased in the yield of four minerals (magnesium sulfate, dipotassium phosphate, potassium chloride, ferrous sulfate) of the existing nitrogen source sodium nitrate and the basic medium CDB including fructose selected as a carbon source and soy flour selected as a nitrogen source. The test was conducted according to the PBD (Plackett-Burman Design) to find out the factors influencing. PBD is a statistical method of knowing how the media component used affects the growth, and it can find out the components of the media component that exhibit the main effect.

PBD uses 12 test groups and 3 reference groups as shown in Table 5 below, and 12 run groups were used for 12 run groups with + run for 200% of medium components and -run for 20% of medium components. Make three badges (reference group) consisting of six randomized and -6 randomized badges and a median of zero. Numbers 1-12 are due to the randomized media composition of + and-, and 1315 is the media composition by the median. With respect to the spore density values obtained for each of these runs, the standard deviation shows how the increase in the run value affects the values. The main effect is indicated by subtracting the -run value from the standard deviation value of + run for each component.The higher the standard deviation value is, the more important the media component is.The increase in this component is absolutely helpful for cell growth. It means.

Plackett-Burman Design (PBD) applied to this study Run Fructose Soybean
meal Magnesium
sulfate Sodium
nitrate Dipotassium phosphate Potassium chloride Ferrous
sulfate One + (30) -(3.0) + (5.0) -(0.3) -(0.1) -(0.5) + (0.01) 2 + (30) + (30) -(0.5) + (3.0) -(0.1) -(0.5) -(0.001) 3 -(3.0) + (30) + (5.0) -(0.3) + (1.0) -(0.5) -(0.001) 4 + (30) -(3.0) + (5.0) + (3.0) -(0.1) + (5.0) -(0.001) 5 + (30) + (30) -(0.5) + (3.0) + (1.0) -(0.5) + (0.01) 6 + (30) + (30) + (5.0) -(0.3) + (1.0) + (5.0) -(0.001) 7 -(3.0) + (30) + (5.0) + (3.0) -(0.1) + (5.0) + (0.01) 8 -(3.0) -(3.0) + (5.0) + (3.0) + (1.0) -(0.5) + (0.01) 9 -(3.0) -(3.0) -(0.5) + (3.0) + (1.0) + (5.0) -(0.001) 10 + (30) -(3.0) -(0.5) -(0.3) + (1.0) + (5.0) + (0.01) 11 -(3.0) + (30) -(0.5) -(0.3) -(0.1) + (5.0) + (0.01) 12 -(3.0) -(3.0) -(0.5) -(0.3) -(0.1) -(0.5) -(0.001) 13 0 (16.5) 0 (16.5) 0 (2.75) 0 (1.75) 0 (0.55) 0 (2.75) 0 (0.0055) 14 0 (16.5) 0 (16.5) 0 (2.75) 0 (1.75) 0 (0.55) 0 (2.75) 0 (0.0055) 15 0 (16.5) 0 (16.5) 0 (2.75) 0 (1.75) 0 (0.55) 0 (2.75) 0 (0.0055)

* The values in parentheses indicate the content of each component (g / L)

In addition to the optimal carbon source fructose and the optimum nitrogen source soy flour, PBD technology was applied to find out which mineral source is needed to improve the yield of spore production of the Simlyillium lamelicola BCP. As a result, it was found that increasing the content of fructose, soy flour, magnesium sulfate, and potassium chloride is advantageous for the yield of spores, and conversely, it is advantageous to reduce the content of sodium nitrate (FIG. 7). Potassium diphosphate and iron sulfate did not appear to have a significant effect on the yield of spore production.

Composition of liquid culture medium of S. simillium lamelicola BCP strain ingredient Concentration (g / L) Content (w / v%) fruit sugar 30.0 3 Soy flour 30.0 3 MgSO ₄ ㆍ 7H ₂ O 5.0 0.5 KCl 5.0 0.5 K ₂ HPO ₄ 1.0 0.1 FeSO _{4 7} H ₂ O 0.01 0.001

By combining the results of the experiment to determine the optimum medium composition as shown in Table 7. The amount of magnesium sulfate and potassium chloride, which was considered to be advantageous in the PBD results, was set to 0.5 w / v% higher than 0.05 w / v% based on the CDB content. Instead sodium nitrate (sodium nitrate), which was considered advantageous in reducing the content, was removed from the medium composition. Potassium diphosphate (dipotassium phosphate) and ferrous sulfate (ferrous sulfate), which did not show a major effect in PBD, were set to the same content as CDB. According to the PBD results, it was found that increasing the content of fructose and soy flour was advantageous to increase the yield of spore production, but according to the results of concentration, there was a risk of inhibitory effect when the concentration was increased. .

Optimal medium composition ingredient Content (g / L) Content (w / v%) fruit sugar 30.0 3 Soy flour 30.0 3 Magnesium Sulfate 5.0 0.5 Potassium chloride 5.0 0.5 Dipotassium phosphate 1.0 0.1 Ferrous sulfate 0.01 0.001

8 and 9 show the results of culturing the Simlyillium lamelicola BCP with the optimum medium composition and PDB (potato dextrose medium) shown in Table 7 above.

Claims (10)

At least one carbon source selected from the group consisting of fructose, dextrin and sucrose; And
One or more nitrogen sources selected from the group consisting of soybean meal and tryptone
Simplicillium lamelilla BCP ( Simiplicillium lamellicola BCP) strain culture medium composition comprising a as a medium component.
The method of claim 1,
At least one mineral selected from the group consisting of magnesium sulfate, potassium chloride, magnesium chloride, sodium chloride, iron sulfate, calcium chloride, potassium diphosphate, copper sulfate, zinc sulfate, potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, potassium iodide and sodium molybdate A culture medium composition for the culture of simplicity lamella cola BCP strain further comprising a circle.
The method of claim 1,
The culture medium composition for simplicity lamella cola BCP strains, characterized in that the medium contains 1.0 to 5.0 w / v% carbon source and 0.5 to 4.0 w / v% nitrogen source.
The method of claim 1,
The badge is 1.0 To 5.0 w / v% fructose; Soy flour of 0.5 to 4.0 w / v%; A culture medium composition for simplicity lamella cola BCP strains comprising 0.1 to 1 w / v% of magnesium sulfate as an essential medium component.
The method of claim 4, wherein
0.1 to 1 w / v% potassium chloride; 0.05-0.5 w / v% potassium diphosphate; And 0.0001 to 0.01 w / v% of iron sulfate.
A method of culturing the Simpliclium lamellicola BCP strain in the medium composition of any one of claims 1 to 5.
The method according to claim 6,
Method for culturing the simplicity lamellicola BCP strain, characterized in that the medium is inoculated with 0.1 to 5 w / v% of the simplicity lamellicola BCP.
The method according to claim 6,
Method for culturing the Simpliclium lamella cola BCP strain, characterized in that the culture temperature is 15 to 37 ℃.
The method according to claim 6,
Method for culturing the Simpliclium lamella cola BCP strain, characterized in that the culture pH is 3 to 8.
The method according to claim 6,
A method of culturing the Simlyillium lamella cola BCP strain, characterized in that the culture period is 20 hours or more.

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