KR100894528B1 - Skin care agent - Google Patents

Abstract

The present invention relates to a skin care accelerator which can improve skin moisturization by oral ingestion and obtain smooth skin, and contains an enzyme decomposition product of lead as an active ingredient. The enzymatic digest is preferably a digest by protease, and the molecular weight of the enzymatic digest is preferably 2,000 to 70,000. The skin care promoter preferably contains collagen and / or enzymatic digests thereof. Furthermore, it is preferable to contain at least 1 sort (s) chosen from a glucosamine, a nucleic acid, a SOD type substance, a SOD activity inducing substance, and lactoferrin. Furthermore, it is preferable to contain at least 1 sort (s) of culture obtained by culturing lactic acid bacteria, enterococci, yeast or fungi.

Skincare, Yeonwa, Enzyme Degradation, Collagen, Nucleic Acid, Glucosamine, Lactoferrin, SOD

Description

Skin Care Accelerator {SKIN CARE AGENT}

1 is a test subject no. The figure which shows the micrograph of the skin replica of 1.

2 is a test subject no. The figure which shows the micrograph of the skin replica of 2.

3 is a test subject no. The figure which shows the micrograph of the skin replica of 3.

4 is a test subject no. The figure which shows the micrograph of the skin replica of 4.

5 is a test subject no. The figure which shows the micrograph of the skin replica of 5.

6 is a test subject no. The figure which shows the micrograph of the skin replica of 6.

7 is a test subject no. The figure which shows the micrograph of the skin replica of 7.

8 is a test subject no. The figure which shows the micrograph of the skin replica of 8.

9 is a test subject no. The figure which shows the micrograph of the skin replica of 9.

The present invention relates to a skin care promoter that can improve skin moistness by oral ingestion and obtain a smooth skin.

Skin problems such as rough skin or fine lines are a serious problem, especially for women. Acidic mucopolysaccharides and collagen, such as hyaluronic acid and chondroitin sulfate, are known as components that are highly involved in skin's water retention and elasticity in skin tissue.

The acidic mucopolysaccharide has a high water retention and binds to collagen, which acts as a support for the intercellular matrix, and is widely distributed in connective tissue, cartilage tissue or skin tissue to help maintain the function or shape of the cells. It is becoming.

In addition, as their age decreases, the conservative properties and elasticity of the skin are lost, resulting in rough skin or fine lines.

Therefore, in order to prevent and improve rough skin or fine wrinkles, it is important to maintain the moisture and elasticity of the skin.

Background Art Conventionally, cosmetics and beauty health foods incorporating various components having a skin moisturizing property and elasticity retention effect are commercially available. Such components include, for example, mucopolysaccharides such as hyaluronic acid and chondroitin sulfate, proteins such as collagen, low molecular sugars such as trehalose and sorbitol, vitamins, amino acid derivatives, ceramides and α-olizanols. And fats and oils such as refined camellia oil, and in particular, there is a tendency to respect components of natural origin with high safety recently.

Specifically, a beauty health food (Japanese Patent Laid-Open No. 10-165138), an active oxygen scavenging factor, an antiallergic factor, skin, etc., containing mucopolysaccharides containing hyaluronic acid and chondroitin sulfate, and collagen and nucleic acid Food consisting mainly of processed foods (Japanese Patent Laid-Open No. 10-70), conchiolin, or a processed product thereof, the main ingredient of which is a mixture of two or more kinds of food ingredients containing improving factors and antioxidant factors (Japanese Patent Application Laid-Open No. 8-70). 173091), health foods having complex mucopolysaccharides in which mucopolysaccharides and peptides are bound (Japanese Patent Laid-Open No. Hei 9-98739), health foods containing ceramide (Japanese Patent Laid-Open No. Hei 11-13530), and the like.

However, cosmetics containing the above ingredients can increase the water retention of the skin surface, but the effect is only temporary, and did not fundamentally improve the condition of the skin.

In addition, the effect was not necessarily satisfactory even if the ingested beauty health food.

Accordingly, it is an object of the present invention to provide a skin care promoter which can increase the water retention of the skin by oral ingestion, thereby obtaining moist and smooth skin.

The present inventors earnestly researched to achieve the above object, and found that by ingesting the enzyme decomposition product of the yeonwa, the skin feels moist and there is little dropping. Reached.

That is, the skin care promoter of the present invention is characterized by containing an enzymatic degradation product of a kite as an active ingredient.

In this invention, it is preferable that the said enzyme degradation product is a degradation product by a protease.

Moreover, it is preferable that the molecular weights of the said enzyme degradation products are 2,000-70,000.                     

According to the present invention, it is possible to provide a skin care promoter which can increase the water retention of the skin by oral ingestion to obtain a moist and smooth skin.

The skin care promoter of the present invention preferably further contains collagen and / or enzymatic degradation products thereof. According to this embodiment, the synergistic effect of collagen and / or its enzymatic degradation product with the enzymatic degradation product of the lotus can further enhance the skin care promoting effect.

The skin care promoter of the present invention preferably further contains at least one selected from glucosamine, nucleic acid, superoxide dismutase (SOD) type substance, SOD activity inducer, and lactoferrin. According to this embodiment, the synergistic effect of the enzyme decomposition products of the above-mentioned each component and lead can further enhance the skin care promoting effect.

The skin care promoter of the present invention preferably further contains at least one culture obtained by culturing lactic acid bacteria, enterococci, yeast or fungi. According to this embodiment, the skin care by synergistic effect with various physiologically active substances produced by the various microorganisms, for example, amino acids, organic acids, vitamin B group, oligosaccharides, peptidoglycan, nucleic acid, etc. The promotion effect can be further enhanced.

Embodiment of the Invention

Yeonwa is a nest that cave swallows make their saliva into a thread, and contains a lot of glycoprotein and little lipid.

There are many kinds of commercially available kites, including the removal of dirt, such as hairs and dung, and the like, as well as those formed by collecting bleach and repeatedly bleaching and washing. It is preferable to use a soft wire which has not been subjected to excessive washing or bleaching in the pretreatment.

Enzymatic digestion of the edible which is an active ingredient of the skin care promoter of the present invention can be obtained by decomposing the edible or processed product of the edible (eg, water extract of the edible) using a protease or a complex enzyme containing a protease.

Preparation of the enzymatic digestion of smoke can be carried out as follows, for example. That is, after swelling by adding 10-50 times the mass of water to the pulverized lead, it heat-processes for 5 second-30 minutes at 60-130 degreeC. Thereafter, an appropriate amount of enzyme is added to carry out the enzymatic reaction at the optimum pH and the optimum temperature of the enzyme for 0.5 to 48 hours. After the enzymatic reaction is completed, the enzyme is deactivated by heat treatment or the like, and the reaction solution is filtered to remove the insolubles. In addition, this enzyme decomposition product can be dried and powdered as needed. Moreover, at each said process, operation, such as pH adjustment, decolorization, and deodorization, can also be performed suitably.

On the other hand, when enzymatically decomposing the water extract of the edible fruit, 10-100 times of its mass is added to the pulverized edible fruit having a particle diameter of 2 mm or less, preferably 150 μm or less, and then 1 to 60 ° C. for 0.5 to 48 hours. What is necessary is just to carry out the extraction by standing still or stirring, heat-processing 60-130 degreeC for 5 second-30 minutes, and enzyme-processing by the method similar to the above using the water extract obtained by filtering as needed.

500-200,000 are preferable and, as for the molecular weight of the enzyme degradation product of the lead obtained as mentioned above, 2,000-70,000 are more preferable.

The enzyme decomposed product of the lead obtained as described above can be used as the skin care promoter of the present invention as it is. Further, excipients, sweeteners, flavorings, colorings and the like may be added to make granules, tablets, capsules and the like.

The effective intake of the skin care promoter of the present invention is 10 to 5,000 mg per person per day as enzyme degradation product (in terms of dry matter). Therefore, the enzyme degradation product content of the yeonwa in the skin care promoter of the present invention preferably contains 10 to 5,000 mg of the enzyme degradation product in terms of dry matter, and 50 to 500 mg to be ingested. More preferred.

In addition, the skin care promoter of the present invention preferably further contains collagen and / or enzymatic degradation products thereof. Collagen can be used without particular limitation as long as it is of a quality usable for food. From the viewpoint of digestion and absorption, enzymatic digest of collagen is more preferred. Enzyme digest of collagen can be prepared by decomposing collagen with an enzyme such as protease. There is no restriction | limiting in particular in the conditions of enzyme decomposition, What is necessary is just to perform an enzyme reaction at the most suitable pH and the most suitable temperature of the enzyme to be used. In addition, a commercially available enzymatic digest of collagen may be used, and for example, "water-soluble collagen peptide PA" (trade name, manufactured by Suwa Kogyo Co., Ltd.) may be used.

The content of collagen (in terms of dry matter) in the skin care accelerator of the present invention is preferably contained so as to be ingested in a range of 0.1 to 10 g, more preferably 1 to 6 g per day.                     

Collagen has been conventionally used in cosmetic health foods, and synergistic skin care effects can be expected by using in combination with the enzyme decomposition products of the yeonwa.

In addition, the skin care promoter of the present invention may contain at least one selected from glucosamine, nucleic acid, SOD type substance, SOD activity inducing substance, and lactoferrin.

Glucosamine is a natural amino sugar, widely distributed as a constituent of mucopolysaccharides such as cartilage and connective tissue in the animal body, and can be expected to have a synergistic skin care effect by being used in combination with an enzyme decomposition product of a kite.

It is preferable to contain content of glucosamine (dry matter conversion) in the skin care promoter of this invention so that it may ingest | ingest 0.5 g or more per day, More preferably, 1.0-2.0 g.

Nucleic acid can be used without particular limitation as long as it is generally used in foods, for example, acid, alkali decomposition or enzymatic treatment of white protein derived from white porcelain such as salmon (for example, trade name "DNA na", The product made by Nippon Chemical Co., Ltd., the brewer's yeast acid, and the alkali decomposition (for example, brand name "yeast ribonucleic acid", the product made by Kirin Viru company), etc. are mentioned.

Nucleic acid is known to have an anti-aging effect on the skin, and synergistic skin care effects can be expected by using it in combination with enzyme degrading products.

It is preferable to contain content of a nucleic acid (dry matter conversion) in the skin care promoter of this invention so that it can ingest 0.5-2.0g, More preferably, 1.0-1.5g per day.                     

The SOD-type substance refers to an antioxidant having the same activity as a superoxide dismutase, and examples thereof include β-carotene, vitamin E, vitamin C and the like.

In addition, SOD activity inducing substance means the substance which has the effect | action which raises the SOD type activity in a living body by ingesting, for example, "oxycaine" (brand name, a sales company: Combi Kaisha) etc. which used melon extract concentrate as a raw material. Can be mentioned.

By ingesting SOD-type substances or SOD activity inducing substances, the free radicals generated in vivo can be eliminated. Therefore, synergistic skincare effects can be expected by using them with enzyme degradants.

The content of the SOD-type substance in the skin care promoter of the present invention is preferably contained such that β-carotene can be ingested at least 10 mg, more preferably 15 to 30 mg per day. In the case of vitamin E, it is preferable to contain 100 to 1,000 mg, more preferably 300 to 700 mg per day, and for vitamin C, 50 to 3,000 mg per day, more preferably 100 to It is desirable to contain 2,000 mg for consumption.

The content of the SOD activity inducing substance in the skin care promoter of the present invention is preferably contained so as to ingest 5 to 1,000 mg, more preferably 100 to 500 mg per day.

Lactoferrin is an iron-binding glycoprotein having a molecular weight of about 80,000 contained in mammalian milk and the like. Lactoferrin has the effect of moisturizing the skin by oral ingestion, and synergistic skin care effect can be expected by using in combination with the enzyme decomposition products of the lotus.

The content of lactoferrin (in terms of dry matter) in the skin care accelerator of the present invention is preferably contained so as to be ingested at least 0.5 g, more preferably 1 to 1.5 g per day.

In addition, the skin care promoter of the present invention may contain at least one culture obtained by culturing lactic acid bacteria, enterococci, yeast or fungi.

As the lactic acid bacteria Lactobacillus Bulgaria kusu (Lactobacillus bulgaricus), Lactobacillus Kasei (Lactobacillus casei), Lactobacillus liquid filler's attempt (Lactobacillus acidophilus), Lactobacillus lactis (Lactobacillus lactis ), Streptococcus thermophilus , and the like are preferable. As the enterococci, enterococcus faecalis and the like are preferable. As said yeast, Saccharomyces serevisiae etc. are preferable. As the fungus, Aspergillus oryzae , Aspergillus sojae , etc. are preferable.

Moreover, as said microorganism culture medium, the following medium is mentioned, for example.

As a medium for lactic acid bacteria or enterococci culture, the medium containing 0.5 mass% of yeast extract, 1 mass% of glucose, and 1 mass% of polypeptone is mentioned as a preferable thing, for example.

As a yeast culture medium, the medium containing 20 mass% of rice leachate and 0.5 mass% of yeast extract is mentioned, for example.

As a fungal culture medium, the medium containing 20 mass% of potato leachate, 2 mass% of glucose, and 0.5 mass% of yeast extract is mentioned as a preferable thing, for example.

In addition, the potato leaching solution is to remove 20 to 100 g of peeled potatoes, add water to it, boil for 1 hour, filter with a cloth, and then add water to make 1,000 ml. In addition, the rice leachate was made to boil for 1 hour by adding water to 20 g of rice, filtered with a cloth, and then to 1,000 ml by adding water.

In the present invention, after sterilization and filtration of the culture medium in which each microorganism is cultured using the medium as described above, it can be appropriately concentrated, or dried and powdered. Cultures of such microorganisms include various physiologically active substances such as amino acids, organic acids, vitamin B groups, oligosaccharides (galacto oligosaccharides, fructo oligosaccharides, etc.), peptidoglycans, nucleic acids, and the like. By using in combination with the enzyme decomposed product of, and can be expected synergistic skin care promoting effect. In addition, what is necessary is just to set culture conditions suitably according to the microorganism to culture.

The content of the culture (in terms of dry matter) in the skin care promoter of the present invention is preferably 10 g or more, more preferably 20 to 40 g per day.

The skin care promoter of the present invention may be taken as it is as a medicine, nutritional supplement, or the like, or may be added to various foods such as beverages, jelly, gummi, candy, gum, snack confectionery, baked confectionery, retort food, and instant food. You may.

The preferred amount of the skin care promoter of the present invention to the food is 10 to 5,000 mg, more preferably 50 to 500 mg, in terms of dry matter of the enzyme decomposition product of lead per meal.

If the amount of enzymatic digestion of lead to food is out of the above range, sufficient skin care effect may not be obtained or the cost may be too high.

EXAMPLE

Hereinafter, the present invention will be described in detail with reference to Examples.

Example

A commercially available unbleached wort was pulverized by a grinder to obtain a wort and powder of 100 mesh passages (150 µm or less in particle diameter). About 50 times (mass) of water was added to the wand and the powder was swollen at 5 ° C. for 3 to 24 hours. After that, heat treatment was performed at 121 ° C. for 15 minutes.

After cooling this process liquid, pH was adjusted and the protease containing enzyme (brand name "pancreatin F", product made from Tenno Corporation) of 2 mass% with respect to lead and powder was added, and it was made to react at 45 degreeC for 3 hours. .

The reaction solution was adjusted to pH 7.0, heated at 90 ° C. for 5 minutes to deactivate the enzyme, and then filtered to recover the filtrate. The filtrate was concentrated, and then freeze-dried to obtain an enzymatic digest of lead.

Test Example

Sample: ① Granule formulation (1.5 g / 1 sachet) containing 24 mass% of enzyme digest of lead and other excipients obtained in Example

② Granular preparation (1.5 g / 1 sachet) containing 24 mass% of enzymatic digest of lead and 42.94 mass% of enzymatic digest of collagen, and other excipients.

③ Granule preparation (1.5 g / 1 sachet) containing 42.94 mass% of enzymatic digest of collagen and other excipients

As the enzymatic digest of collagen, "water-soluble collagen peptide PA" (trade name, manufactured by Suwa Kogyo Co., Ltd.) was used. In addition, each sample contained reduced maltose syrup, dextrin, stevia, calcium phosphate as an excipient etc. in the same ratio, and erythritol was used for adjustment of mass.

Test Method: Nine healthy women in their 20s to 50s were treated with Test Group 1 (enzyme digest group fed with kites), Test Group 2 (Enzyme digest group with collagen + enzymatic digesters administered with collagen), and Comparative group (enzyme digest administered groups with collagen). Each sample was taken continuously for 4 days at 4 bags / day (6 g / day).

Evaluation method: The replica of the skin surface of the subject was sampled before the test start (TO) and at the end of the test (T2), respectively. In addition, replica collection was carried out by each subject using the dermotest "DIAGNOSKIN" (brand name, the BIO-EC Laboratories company) which is a replica collection kit. Specifically, the dermotest flake having a circular adhesive portion was lightly pressed against the skin surface of the forearm inner portion for about 30 seconds, and a replica of the skin surface was collected on the adhesive portion.

Each replica was inspected and photographed by an optical microscope, and these replicas were evaluated according to the evaluation criteria shown in Table 1. The evaluation criteria were scored based on the database of BIO-EC Laboratories.                     

TABLE 1

 score  Condition of the skin  replica  0  Very dry  Many large snowfalls have been identified.                                               There is a very thick and large mass of cells.  3  dry  Clearly visible snowfall is identified.                                               There are few cells isolated. No small cells There are a large number of slightly thicker, larger cells.  6  normal  Small snowfall is confirmed.                                               There are several cells isolated. There are clumps of thin, different sized cells.  9  Moisturizing  No surprises                                               There are many isolated cells, and there are several thin and large cell masses.  12  Moisturizing very good Small cell clumps ^* There are multiple. There are many isolated cells. No thickness

*; Refers to a mass consisting of at least 20-30 cells.

The results are shown in Table 2. Further, the score in the table means that the higher the score, the higher the moisture level of the skin, and the better the condition of the skin. The lower the score, the higher the dryness of the skin and the worse the condition of the skin.

TABLE 2

 Subject  Score  evaluation (T0) (T2) (Comparison of TO and T2)  Test group 1 No. 1 (22 years old) 7 11 Improvement medium No. 2 (age 33) 6 8 Less improvement No. 3 (age 51) 7 10 Less improvement  Test group 2 No. 4 (age 22) 9 11 Less improvement No. 5 (age 37) 5 9 Improvement medium No. 6 (56 years old) 5 11 Improvement large  Comparison No. 7 (age 20) 6 8 Less improvement No. 8 (age 29) 9 7 Less improvement No. 9 (37 years old) 6 6 No change

From the result of Table 2, it turns out that the test group 1 and the test group 2 tend to improve the condition of skin compared with a comparative group. Especially in the test group 2 which ingested the enzyme degradation product of Yeonwa and the enzyme degradation product of collagen, it turns out that the improvement of skin condition is large.

Moreover, the optical micrograph of the skin replica of each subject is shown to FIGS. 1 to 3 are for test group 1, 4 to 6 for test group 2, and 7 to 9 for comparative groups. (A) is a replica before the test (T0), and (b) is a replica at the end of the test (T2).

In Figs. 1 to 6 (test groups 1 and 2), it can be seen that the drop-off in the state at the end of the test is reduced and the skin condition is improved compared to the state before the test. On the other hand, in FIGS. 7-9 (comparative group), it turns out that a change hardly appears in the state before a test and the state at the end of a test.

As described above, according to the present invention, it is possible to provide a skin care promoter capable of improving moisturized skin and obtaining moist, smooth skin by containing an enzyme decomposition product of a kite as an active ingredient.

Claims (6)

A skin care accelerator, comprising an active ingredient containing a degradation product of pancreatin of Yeonwa. The method of claim 1, A skin care promoter having a molecular weight of 2,000 to 70,000 of the decomposition product. The method according to claim 1 or 2, Collagen, its enzymatic digest, or skin care promoter, which further contains collagen and its enzymatic digest. The method according to claim 1 or 2, A skin care promoter further comprising at least one member selected from the group consisting of glucosamine, nucleic acids, superoxide dismutase (SOD) type substances, and lactoferrin. The method according to claim 1 or 2, A skin care promoter containing at least one further culture obtained by culturing lactic acid bacteria, enterococci, yeast or fungi. delete

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