KR100849660B1 - Ginseng preparations containing a high concentration of ginseng sapogenin using a Bacillus natto and method thereof - Google Patents

Abstract

The present invention relates to a ginseng preparation containing high concentration of ginseng sapogenin using Bacillus natto bacteria and a method for preparing the same. 24 ~ 96 hours and more particularly to the ginseng or ginseng X boiled bean mixture to contain 10 to 30% by weight before a modification of the culture of Bacillus NATO (Bacillus natto) seed culture 1-5% after inoculation, 42 ~ 48 ℃ The present invention relates to a method for preparing a ginseng preparation containing high concentration of ginseng sapogenin by fermentation for a while. According to the present invention, since 0.1 to 10.5% by weight of protopanaxadiol, which is a functional substance, and 0.01 to 1.2% by weight of protopanaxatriol, the functional material is superior to conventional ginseng. The ginseng preparation which has is provided.

Ginseng Sapogenin, Bacillus natto, Protopanaxadiol, Protopanaxatriol

Description

Ginseng preparations containing a high concentration of ginseng sapogenin using a Bacillus natto and method according to Bacillus natto

Field of technology

The present invention relates to a ginseng sapogenin high concentration formulation containing ginseng and a method using a NATO Bacillus (Bacillus natto) strains.

Prior art

Korean ginseng ( Panax ginseng ) is the main specialty product of Korea, which is collected in the products of Shin Nongshim Co., Ltd., the best herbal book of the East. Korean ginseng contains about 30 kinds of ginseng saponins, polyacetylene with anticancer activity, phenolic compounds with antioxidant action, ginseng protein with radiation defense action, acidic polysaccharides with immunomodulatory action, and the like. In particular, ginseng saponin, known as the main bioactive component of Korean ginseng, is called ginsenoside, and its chemical structure has been confirmed by the Shibata group of the University of Tokyo. Ginsenosides are classified into protopanaxadiol and protopanaxatriol based saponins according to the number of alcoholic OHs of aglycone, C-3 and C-20 or C-6 and Sugars such as glucose, rhamnose, xylose and arabinose are ether-bonded to form the ginseng saponin glycosides. The main ginseng saponin glycoside component of the ProtoPanaxadiol group is ginsenoside Rb ₁ , which indicates central nervous system suppression, and the main ginseng saponin glycoside component of the ProtoPanax triol group is ginsenoside Rg _{1, which} produces central nervous system excitability. It is known to contribute to the adaptogen activity of Korean ginseng.

In Korean ginseng, the content ratio (diol / triol) of the ProtoPanacodiol group and ProtoPanacotri triol group and the content ratio (Rb ₁ / Rg ₁ ) of Ginsenoside Rb ₁ and Ginsenoside Rg ₁ were 1.96 and 3.14, which is more balanced than the western ginseng's 2.48 and 25.96, which exhibits excessive central nervous system suppression, but exhibits a true viewing rather than excitement. Therefore, Korean ginseng is a herbal pharmacological effect, which is a balanced and stable dexterity of the ginsenoside Rb ₁ and the ginsenoside Rg _{1, which} are both stable and stable. It is considered to be the best tonic of modern people in the sense.

The pharmacological activities of Korean ginseng so far identified include reinforcement of heart and blood vessels, recovery of blood function, improvement of blood flow, prevention of arteriosclerosis and hypertension, enhancement of gastrointestinal control, promotion of liver function and hangover, anti-fatigue and anti Stress action, anti-aging action, brain activity promoting action, anti-inflammatory activity, allergic disease treatment, gynecological disease, diabetes mellitus, anti-radiation action, energy enhancement, anti-tumor action, improvement of learning ability, inhibition of production of lipid oxide, promotion of wound treatment, Improving immune capacity, inhibiting AIDS virus growth, and promoting protein synthesis have been reported.

In addition, red ginseng extracted from fresh ginseng from the field is removed, and red ginseng is dried naturally in daylight and white ginseng is dried. In particular, red ginseng is produced by heat, and the small amount of ginsenosides such as Rg ₂ , Rg _3, Rh _1, Rh _{2, and} Rh ₄ has the effect of preventing cancer, inhibiting cancer metastasis, lowering blood pressure, and antioxidant activity. It is attracting attention as a feature and advantage of red ginseng.

As mentioned above, Korean ginseng has been recognized as the best health product for a long time, but currently occupies 3% market share in the global ginseng market. Therefore, in order to innovate the domestic ginseng industry, it is necessary to develop high value-added ginseng products. Nevertheless, ginseng preparations and red ginseng preparations up to now have been developed at the level of tonics, with a simple extract level preparation. However, in order to develop more professional and functional ginseng preparations, the development of ginseng preparations containing high concentrations of functional materials with strong physiological activities and ensuring safety will be essential.

For this reason, a method for making a ginseng preparation containing a large amount of ginseng saponins unique to red ginseng has recently been tried. According to the Shibata paper published in 1966, hydrolysis of saponins with weak acids such as acetic acid only hydrolyzed the glycoside bonds of g-20 (ginsenosides Rb ₁ , Rb ₂ , Rc, Rd), and prosapogenin ( It was announced that 20 (R & S) -ginsenoside Rg ₃ ) was obtained, but this was only to make a standard. In addition, other researchers have used a method of ginsenoside Rg ₃ containing a large amount of enzyme treatment the physical reaction of high-temperature treatment or biochemical reaction to make a ginseng preparation.

The method of high temperature treatment relates to processed ginseng, for example, which is enhanced by heating ginseng at a high temperature. Ginsenoside (Rg ₃ + Rg ₅ ) is heated by heating ginseng at a temperature of 120 to 180 ° C. for 0.5 to 20 hours. Patents on processed ginseng products with increased active ingredient so that the ratio of / (Rc + Rd + Rb ₁ + Rb ₂ ) is 1 or more (Korean Patent No. 192678) and saponin glycoside degrading enzyme There is a patent (Korean Patent No. 329259) on the preparation of rare anticancer saponins (ginsenosides Rh ₁ , Rh ₂ ) by hydrolysis of ginseng saponins, and currently commercially available as sun ginseng and new ginseng. It is becoming.

These ginseng-specific ingredients (ginsenosides Rg ₂ , Rg _3, Rh _1, Rh _2, Rh ₄ ) are ginseng saponin glycosides (ginsenosides Rb ₁ , Rb ₂ , Rc, Rd) present in white ginseng and fresh ginseng. , Re, Rf, Rg ₁ ) The part of the sugar (dam) bound to the triterpenoid structure of dammaran can be said to be a free ginseng prosapogenin component.

In other words, when ginseng saponin is hydrolyzed with weak acid such as heat or acetic acid, only G-20 glycosidic bond is hydrolyzed and prosapogenin is obtained. Thus, ginsenoside-Rb ₁ , -Rb ₂ , -Rc, -Rd and the like yield 20 (R & S) -ginsenoside Rg ₃ which is the same prosapogenin. Ginsenosides Re, 20-ginsenosides Rf, and ginsenosides Rg ₁ , such as the protoparanaxatriol-based saponins, also have different types of prosapogenin obtained by thermal or weak acid hydrolysis of C-20 glycoside bonds. That is, in the case of ginsenoside Re, 20 (R & S) -ginsenoside Rg ₂ is produced.

However, the development of a preparation containing a high concentration of ginseng saponenin (protopanaxadiol, protofanaxatriol) in which sugar is not bound to the ginseng saponin dammaran triterpenoid base nucleus has not been made yet.

Therefore, the inventors of the present invention have been conducting continuous research to prepare a ginseng preparation containing a high concentration of functional substances such as ginseng sapogenin (protopanaxanadiol, protopanaxanatriol) and a processing method for treating ginseng with a weak acid; Unlike, inoculated 1 ~ 5% of Bacillus natto seed culture medium cultured with boiled soybeans containing 10 to 30% by weight of ginseng, and then fermented at 42 to 48 ℃ for 24 to 96 hours to freeze-dried. Korean ginseng sandpaper The present invention has been completed by developing a ginseng preparation containing high concentration of genes.

As described above, the present invention has a significantly higher cell affinity than the existing ginseng component, and the drug efficacy is increased from 0.1 to 10.5% by weight based on the weight of the lyophilized ginseng preparation, protopanaxatriol (protopanaxatriol). It is an object of the present invention to provide a ginseng preparation containing a high concentration of 0.01% to 1.2% by weight relative to the weight of the lyophilized product and a method for economically manufacturing the same.

The present invention is characterized by maximizing the content of the active ingredients in ginseng and the content of rare ginseng components by using natural soybean fermentation, and at the same time, the active ingredients in soybeans remain in the ginseng preparation to enhance and supplement the effect.

Referring to the production method of the ginseng preparation according to the present invention in more detail as follows.

For life by mixing a soya or Ginseng X around the cultured Bacillus NATO (Bacillus natto) seed culture 42 ~ 48 ℃, for 24 to 96 hours, more preferably 24 ~ 48 hours after the inoculation of 1 to 5%, It is fermented and then lyophilized to obtain a ginseng preparation. NATO Bacillus (Bacillus natto) 1-5% seed culture is prepared by inoculating a Bacillus strain to contain 1-5% by volume of the main culture medium. When the fermentation temperature is out of the fermentation there is a problem that the fermentation reaction is inhibited. In addition, when the fermentation time exceeds 96 hours in the fermentation, the desired active ingredient in the final obtained product is obtained in a very small amount, when the treatment is less than 24 hours, the content of the active ingredient is also lowered and there are many difficulties in operating the manufacturing process. At this time, the ratio of mixing ginseng to soybean is preferably 10 to 30% by weight, more preferably 10 to 15% by weight based on the weight of the bean. At this time, if the content is less than 10% by weight, there is a problem that the yield of ginseng active target component is lowered, and if it exceeds 30% by weight, the fermentation reaction is inhibited.

The ginseng preparation prepared by the method of the present invention is 0.1 ~ 10.5% by weight based on the weight of the freeze-dried ginseng preparation, and 0.01 ~ 1.2% by weight based on the weight of the freeze-dried ginseng preparation. It has been confirmed that it can be useful for improving blood circulation, erectile dysfunction, fatigue recovery, hypertension, arteriosclerosis, antithrombosis, stroke treatment and prevention, and brain function improvement.

In the present invention, which part of the ginseng is used cannot be an important factor in determining the effect of the present invention. Thus, the term ginseng in the present invention, Ginseng (Panax ginseng) for including seoyangsam (Panax quinquefolium), samchil sam (Panax notoginseng) and Panax (Panax) in the ground or below-ground herbal plants as, for example, misam, white ginseng , Red ginseng, ginseng, taegeuk ginseng, ginseng leaf, ginseng fruit and the case of processing them are included, based on the facts confirmed from repeated experiments related to ginseng.

In addition, the soybean (soybean, Glycine max ) that is characteristically used in the present invention may use seeds or pods of plants belonging to the legume.

The ginseng preparation of the present invention may be powdered through a conventional powdering process or manufactured in the form of a pill or capsule to extend the shelf life, and may be prepared in the form of a liquid ginseng in the form of a beverage.

Hereinafter, the present invention will be described in more detail based on the following examples, but the present invention is not limited thereto.

Preparation Example 1 Preparation of Ginseng Extract

50 g of white ginseng and 250 ml of 95% ethanol (alcohol) were added to an airtight container, and the ginseng extract obtained by extracting and filtering four times for 4 hours at 76 ° C. was dried under reduced pressure to prepare ginseng extract.

Preparation Example 2 Preparation of White Ginseng Powder

White ginseng was put into a grinder to grind into fine powder to prepare white ginseng powder.

Preparation Example 3 Preparation of Red Ginseng Powder

Red ginseng was put into a grinder and ground to a fine powder to prepare red ginseng powder.

Preparation Example 4: Preparation of Fine Ginseng Powder

The fine ginseng powder was prepared by grinding the fine ginseng into a fine powder.

Preparation Example 5 Preparation of Western Gummy Powder

The ginseng was ground in a grinder to crush into fine powder to prepare a ginseng powder.

Preparation Example 6 Preparation of Ginseng Leaf Powder

Dry ginseng leaf was put into a grinder to grind to fine powder to prepare a ginseng leaf powder.

Preparation Example 7 Preparation of Ginseng Extract

50 g of white ginseng and 250 ml of 95% ethanol (weekly) were put into an airtight container, and extracted with four times of 4 hours in a 76 ° C. water bath, filtered, and concentrated to prepare ginseng extract.

Example 1

200 g of boiled soybeans were mixed well with 30 g of white ginseng powder, inoculated with 1-5% by volume of Bacillus natto seed culture solution, and fermented at 42 ° C. for 24 hours. The obtained ginseng preparation was freeze-dried to obtain a brown amorphous mass, which was analyzed by HPLC method.

Example 2

200 g of boiled soybeans were mixed well with 30 g of white ginseng powder, inoculated with 1-5% of Bacillus natto seed culture solution, and fermented at 42 ° C. for 48 hours. The obtained ginseng preparation was freeze-dried to obtain a brown amorphous mass, which was analyzed by HPLC method.

Example 3

200 g of boiled soybeans were mixed well with 30 g of white ginseng powder, inoculated with 1-5% of Bacillus natto culture medium, and fermented at 42 ° C. for 72 hours. The obtained ginseng preparation was freeze-dried to obtain a brown amorphous mass, which was analyzed by HPLC method.

Example 4

200 g of boiled soybeans were mixed well with 30 g of white ginseng powder, inoculated with 1-5% of Bacillus natto culture medium, and fermented at 42 ° C. for 96 hours. The obtained ginseng preparation was freeze-dried to obtain a brown amorphous mass, which was analyzed by HPLC method.

Example 5

200 g of boiled soybeans were mixed well with 30 g of red ginseng powder, inoculated with 1-5% of Bacillus natto culture medium, and fermented at 42 ° C. for 24 hours. The obtained ginseng preparation was freeze-dried to obtain a brown amorphous mass, which was analyzed by HPLC method.

Example 6

200 g of boiled soybeans were mixed well with 30 g of fine ginseng powder, inoculated with 1-5% of Bacillus natto culture medium, and fermented at 44 ° C. for 24 hours. The obtained ginseng preparation was freeze-dried to obtain a brown amorphous mass, which was analyzed by HPLC method.

Example 7

200 g of boiled soybeans were mixed well with 30 g of Western ginseng powder, inoculated with 1-5% of Bacillus natto culture medium, and fermented at 44 ° C. for 24 hours. The obtained ginseng preparation was freeze-dried to obtain a brown amorphous mass, which was analyzed by HPLC method.

Example 8

200 g of boiled soybeans were mixed well with 30 g of ginseng leaf powder, inoculated with 1-5% of Bacillus natto culture medium, and fermented at 44 ° C. for 24 hours. The obtained ginseng preparation was freeze-dried to obtain a brown amorphous mass, which was analyzed by HPLC method.

Example 9

200 g of boiled soybeans were mixed well with 30 g of ginseng extract, inoculated with 1-5% of Bacillus natto culture medium, and fermented at 44 ° C. for 24 hours. The obtained ginseng preparation was freeze-dried to obtain a brown amorphous mass, which was analyzed by HPLC method.

Example 10

Bacillus natto by mixing 20 g of white ginseng powder with 200 g of boiled beans for 8 hours After inoculating 1-5% of the seed culture solution, the fermentation was carried out at 42 ° C for 24 hours. The obtained ginseng preparation was freeze-dried to obtain a brown amorphous mass, which was analyzed by HPLC method.

Example 11

60 g of boiled soybeans were mixed well with 60 g of white ginseng powder, inoculated with 1-5% of Bacillus natto culture, and then fermented at 42 ° C. for 24 hours. The obtained ginseng preparation was freeze-dried to obtain a brown amorphous mass, which was analyzed by HPLC method.

Example 12

Liquid Culture of Bacillus Nato

A culture solution of the following composition was placed in a 5 L fermentation tank so that the culture solution was 3 L. The culture composition is 4 g of potato infusion solids, 20 g of dextrose per liter of culture. After sterilizing the medium for 20 minutes at 121 ° C. using steam, 150 ml of Bacillus natto seed culture medium was added thereto, and the pH of the culture medium was 7.1 to 7.3, the temperature was 40 ° C. to 42 ° C., and the aeration amount was 0.1 to 1.0 VVM. Aerobic culture was performed for 12-24 hours while maintaining. After the incubation was terminated, 200 g of ginseng extract was added and aerobic culture was performed for 6 hours while maintaining the above conditions. After the incubation was terminated, the resultant was filtered through a 0.45 u cartridge filter, and the filtrate was lyophilized to obtain 170 g of functional ginseng powder.

Example 13

Liquid Culture of Bacillus Nato

A culture solution of the following composition was placed in a 5 L fermentation tank so that the culture solution was 3 L. The culture composition is 4 g of potato infusion solid, 20 g of dextrose per liter of culture. After sterilizing the medium for 20 minutes at 121 ° C. using steam, 150 ml of Bacillus natto seed culture medium was added thereto, and the pH of the culture medium was 7.1-7.3, the temperature was 40-42 ° C., and the aeration amount was 0.1-1.0 VVM. Aerobic culture was performed for 12-24 hours while maintaining. After the incubation was terminated, 200 g of ginseng extract was added and aerobic culture was performed for 12 hours while maintaining the above conditions. After the incubation was terminated, the mixture was filtered through a 0.45 u cartridge filter, and the filtrate was lyophilized to obtain 170 g of functional ginseng powder.

Comparative Example 1

250 ml of 95% ethanol was added to 50 g of white ginseng and extracted twice at 76 ° C for 2 hours. The filtrate was concentrated under reduced pressure and lyophilized to give a brown extract.

Comparative Example 2

200 g of boiled soybeans were mixed well with 30 g of white ginseng powder and fermented naturally at 48 ° C. for 120 hours. The obtained ginseng chungkukjang was lyophilized to obtain a brown amorphous mass, which was analyzed by HPLC method.

Comparative Example 3

200 g of boiled soybeans was mixed well with 30 g of white ginseng powder and fermented naturally at 42 ° C. for 24 hours. The obtained ginseng preparation was freeze-dried to obtain a brown amorphous mass, which was analyzed by HPLC method.

Experimental Example 1: Analysis of ginseng sapogenin components by HPLC method

① Experiment Method

50 g of each sample was treated with ether three times, and the aqueous part was treated with saturated butanol three times, and the resulting n-butanol layer was concentrated under reduced pressure to give crude saponin (Shibata method). The obtained crude saponin was quantified by HPLC method, and the results are shown in the following Tables 1, 2, 3 and 4.

② HPLC analysis conditions

Each ginseng saponin component prepared a calibration curve based on 1 mg / ml (1000 ppm), and each sample was prepared at 10 mg / ml (10000 ppm) and injected.

The HPLC conditions are as follows:

HPLC Model Name: Alltech Binary Gradient HPLC system Model 627

Column: Prevail Carbohydrate ES column (manufacturer: Alltech)

Detector: ELSD detector (Manufacturer: Alltech)

Temperature: room temperature

Mobile phase: Solvent A-ACN: Water: IPA = 80: 5: 15

         Solvent B-ACN: Water: IPA = 67: 21: 12

time 0 28 35 45 50 51 57 58 65 % B 10 85 80 75 90 100 25 10 10

Flow rate: 0.8 ml / min

Table 1. Ginsenoside content of ginseng preparations

division Formulation Comparative Example 1 Example 1 Example 2 Example 3 Example 4 Comparative Example 2 Comparative Example 3 Ginsenoside (Unit: wt%) PPD 0 1.37159 0.23989 0.15730 0.11535 0.00686 0.00843 PPT 0 0.12654 0.01124 0.01432 0.01584 0.00426 0.00635 Rg ₁ 0.20786 0 0.01185 0 0.00194 0.00316 0.00423 Rf 0.04965 0 0 0 0 0 0 Re 0.16321 0 0.00229 0.00280 0 0.00232 0.00258 Rd 0.14359 0 0.02280 0.05983 0.25952 0.10329 0.10214 Rc + Rb ₂ 0.46540 0 0.00267 0.00670 0.00935 0.00689 0.00542 Rb ₁ 0.46352 0 0 0 0 0 0 * PPD-protopanaxadiol, PPT-protopanaxatriol, Rg ₁ -ginsenoside Rg ₁ , Rf-ginsenoside Rf, Re-ginsenoside Re, Rd-ginsenoside Rd, Rc-ginsenoside Rc, Rb ₂ -ginsenoside Rb ₂ , Rb ₁ -ginsenoside Rb ₁

As shown in Table 1, the contents of various ginsenosides by HPLC method were investigated for white ginseng extract (Comparative Example 1) and ginseng preparation obtained by Shibata method from the ginseng preparation (Example) obtained according to the method of the present invention. As a result of the analysis, white ginseng extract was not detected at all ginseng sapogenin components such as protoparnaxadiol and protoparnaxatriol, whereas in the ginseng preparation of the present invention, a large amount of protoparnaxadiol and protoparnaxatriol Was detected. Particularly, in the case of Example 1, the content of protoparnaxadiol was 1.37159% by weight relative to the weight of the ginseng freeze-dried product, which showed a high content ratio of 91.55% in total saponin. The content of protopanaxadiol was 0.23989% by weight, indicating a high content ratio of 82.51% in total saponins. However, Comparative Examples 2 and 3 in which spontaneous fermentation was selected without inoculating Bacillus natto seed culture solution 1 to 5% contained low concentrations of ginseng sapogenin.

Table 2.

division Formulation Example 5 Example 6 Example 7 Example 8 Example 9 Ginsenoside (Unit: wt%) PPD 1.03450 5.12240 5.05678 6.78923 10.45200 PPT 0.10563 0.50112 0.42345 1.00200 1.16450 Rg ₁ 0 0.01267 0.00366 0.15583 0.15583 Rf 0 0 0 0 0 Re 0 0.00432 0.02569 1.98331 0.25003 Rd 0 0.03248 0.09948 0.43620 1.56222 Rc + Rb ₂ 0 0.00320 0.19832 0.24563 1.75630 Rb ₁ 0 0 0 0 0.78885 PPD-protopanaxadiol, PPT-protopanaxatriol, Rg1-ginsenoside Rg1, Rf-ginsenoside Rf, Re-ginsenoside Re, Rd-ginsenoside Rd, Rc-ginsenoside Rc, Rb ₂ -ginsenoside Rb ₂ , Rb ₁ -ginsenoside Rb ₁

In the results of Table 2, Example 5 showed a similar content as in Example 1, but in Examples 6 and 7, the contents of Protoparnaxadiol were 5.12240% and 5.05678%, respectively, based on the weight of the lyophilized ginseng preparation. It can be seen that the production of diol is greatly increased. In addition, Examples 8 and 9 showed a high concentration of protopanaxadiol at 6.78923% and 10.452% based on the weight of the lyophilized ginseng preparation.

Table 3.

division Formulation Example 10 Example 11 Example 12 Example 13 Comparative Example 3 Ginsenoside (Unit: wt%) PPD 1.02400 0.25470 5.12800 15.25600 0.00843 PPT 0.10221 0.01330 0.26430 1.54330 0.00635 Rg ₁ 0 0.01170 0.00412 0.00212 0.00423 Rf 0 0 0 0 0 Re 0 0.00232 0.00623 0.00523 0.00258 Rd 0 0.02730 0.0232 0.00122 0.10214 Rc + Rb ₂ 0 0.00324 0. 0. 0.00542 Rb ₁ 0 0 0 0 0 * PPD-protopanaxadiol, PPT-protopanaxatriol, Rg ₁ -ginsenoside Rg ₁ , Rf-ginsenoside Rf, Re-ginsenoside Re, Rd-ginsenoside Rd, Rc-ginsenoside Rc, Rb ₂ -ginsenoside Rb ₂ , Rb ₁ -ginsenoside Rb ₁

In the results of Table 3, Example 10 showed a similar content to Example 1, and Example 11 had a significantly lower content of ginseng sapogenin than Example 10, but improved blood circulation, improved erectile dysfunction, and fatigue recovery. Effective contents of sapogenin could be obtained, such as hypertension, arteriosclerosis, antithrombosis, stroke treatment and prevention, or improvement of brain function. In particular, Comparative Example 3, which selected natural fermentation, showed a significantly lower ginseng saponenin content, but Examples 12 and 13 of liquid culture fermentation showed high concentrations of ginseng sapogenin.

Therefore, the present invention is mixed with 10 to 30% by weight of ginseng or ginseng extract in boiled soybeans inoculated Bacillus natto seed culture solution 1-5% and then fermented at 42-48 ℃, 24-96 hours lyophilized sandpaper By preparing a ginseng preparation containing high concentration of genes, 0.1-10.5% by weight of the protopanaxadiol, a functional substance, relative to the freeze-drying weight of the ginseng preparation, and the freeze-drying weight of the protopanaxatriol, a ginseng preparation It was confirmed that ginseng preparations containing 0.01 to 1.2% by weight can be produced. In addition, liquid culture of Bacillus natto to ferment the ginseng extract to replace the functional substance protopanaxadiol (protopanaxadiol) against the ginseng lyophilized weight. 5.1 to 15.3 % By weight, 0.3 to 1.5, based on the weight of lyophilized ginseng protopanaxatriol It was confirmed that ginseng preparations containing weight% could be prepared.

As described above, the present invention can easily prepare a ginseng preparation containing high concentrations of protopanaxadiol and trotofanaxatriol, which are ginseng sapogenin, which are highly functional substances.

Claims (8)

Ginseng or ginseng extract mixed with boiled beans, inoculated 1 ~ 5% of precultured Bacillus natto seed culture broth, fermented at 42 ~ 48 ℃, 24 ~ 96 hours and then lyophilized to produce ginseng preparations. , 0.1 to 10.5 wt% of protopanaxadiol and 0.01 to 1.2 wt% of protopanaxatriol with respect to lyophilized weight. The method of preparing a ginseng preparation according to claim 1, wherein 10 to 30 wt% of ginseng or ginseng extract is mixed with respect to the weight of soybean. The method for preparing a ginseng preparation according to claim 2, wherein 10 to 15 wt% of ginseng or ginseng extract is mixed with respect to the weight of soybean. The method of claim 1, wherein the fermentation is performed for 24 to 48 hours. The method of claim 1, wherein 1 to 10.5% by weight of the protopanaxadiol and 0.1 to 1.2% by weight of the protopanaxatriol are prepared. A ginseng preparation prepared according to any one of claims 1 to 4, wherein 0.1 to 10.5 wt% of protopanaxadiol and 0.01 to 1.2 wt% of protopanaxatriol based on the lyophilized weight of the ginseng preparation Ginseng preparations characterized in that it contains. The ginseng preparation according to claim 6, wherein 1 to 10.5% by weight of protopanaxadiol and 0.1 to 1.2% by weight of protopanaxatriol are contained. Bacillus natto bacteria were cultured in liquid, added with ginseng extract, and then cultured again to contain 5.1 to 15.3 wt% of the protopanaxadiol and 0.3 to 1.5 wt% of the protopanaxatriol. Ginseng preparations characterized in that.