KR100424608B1 - A composition for increasing the yield of mushrooms with low cost - Google Patents
A composition for increasing the yield of mushrooms with low cost Download PDFInfo
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- KR100424608B1 KR100424608B1 KR10-2001-0033160A KR20010033160A KR100424608B1 KR 100424608 B1 KR100424608 B1 KR 100424608B1 KR 20010033160 A KR20010033160 A KR 20010033160A KR 100424608 B1 KR100424608 B1 KR 100424608B1
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Abstract
본 발명은 고품질의 버섯을 저비용으로 다수확하기 위한 버섯 증수개량제 조성물에 관한 것으로, 더욱 상세하게는 옥수수 배아박, 농축된 당밀의 가용성 발효물질(condensed molasses fermentation solubles; 이하, 'CMS'라 함), 바이오스톤, 전기석(tourmaline), 활성탄 및 황산아연으로 구성된 조성물을 버섯 증수개량제로서 사용하는 것에 관한 것이다.The present invention relates to a mushroom thickener composition for harvesting high quality mushrooms at low cost, and more particularly, to corn embryos, condensed molasses fermentation solubles (hereinafter referred to as 'CMS'), A composition consisting of biostone, tourmaline, activated carbon and zinc sulfate is directed to the use as a mushroom thickener.
Description
본 발명은 고품질의 버섯을 저비용으로 다수확하기 위한 버섯 증수개량제 조성물에 관한 것으로, 더욱 상세하게는 옥수수 배아박, 농축된 당밀의 가용성 발효물질(condensed molasses fermentation solubles; 이하, 'CMS'라 함), 바이오스톤, 전기석(tourmaline), 활성탄, 황산아연으로 구성된 조성물을 버섯 증수개량제로서 사용하는 것에 관한 것이다.The present invention relates to a mushroom thickener composition for harvesting high quality mushrooms at low cost, and more particularly, to corn embryos, condensed molasses fermentation solubles (hereinafter referred to as 'CMS'), It relates to the use of a composition consisting of biostone, tourmaline, activated carbon, zinc sulfate as a mushroom thickener.
옥수수 배아박은 가축에 직접 사용하거나 배합사료의 원료로 이용되고 있는 단미사료로서, 단백질 및 에너지원으로 이용되는 기호성이 높은 물질이다. 옥수수 배아박은 일반적으로 옥수수 배아(일명, 옥수수씨 눈)로부터 가열압착법 또는 용매추출법에 의해서 기름을 추출하고 남은 부산물을 일컫는다. 우리나라에서 옥수수 배아박은 연간 90,000톤 정도 생산된다. 옥수수 배아박의 주성분은 조단백질 22±2%, 조지방 3%이하, 섬유소 8.0±1%, 조회분 4.5±1%, 가용성 무기질소분 51±4% 등의 풍부한 영양성분으로 구성되어 있고, 비타민 등의 성분들도 다량 포함되어 있어, 가축의 배합사료로도 사용되고 있다.Corn embryos are short-term feeds that are used directly in livestock or as feedstock for blended feeds. They are highly palatable materials used as proteins and energy sources. Corn embryos generally refer to the by-products of oil extraction from corn embryos (aka corn seed eyes) by heat compression or solvent extraction. In Korea, corn germ meal is produced about 90,000 tons per year. The main components of corn embryos are rich in nutrients such as crude protein 22 ± 2%, crude fat 3% or less, cellulose 8.0 ± 1%, crude ash 4.5 ± 1%, and soluble mineral nitrogen 51 ± 4%. It also contains a large amount of ingredients, and is also used as a feed for livestock.
한편, CMS는 당밀을 주원료로 사용하여, 발효공법을 통해 라이신(lysine)을 생산할 때 수반되는 검은색의 액상 비단백태 질소원으로 통상적으로 라이신 CMS라 일컫는다.Meanwhile, CMS uses molasses as a main raw material, and is commonly referred to as lysine CMS as a black liquid non-protein nitrogen source involved in producing lysine through a fermentation method.
또한, 전기석은 결정편암, 편마암, 접촉변성암, 화강암의 접촉변성암 속에서 나오는 물질로서, 화학조성의 일반식이 WX3B3Al3(AlSi2O9)3(O,OH,F)4로써 나타내어지는 광물이다(여기에서, W=Na, Ca; X=Al, FeⅢ, Li, Mg). 19세기말, 전기석 결정체는 초전성 및 압전성을 나타낸다는 것이 발견되었다.In addition, the tourmaline is a substance that comes out of the contact metamorphic rock of crystalline schist, gneiss, contact metamorphic rock and granite, and the general formula of chemical composition is represented by WX 3 B 3 Al 3 (AlSi 2 O 9 ) 3 (O, OH, F) 4. It is a mineral (where W = Na, Ca; X = Al, Fe III , Li, Mg). At the end of the 19th century, it was discovered that tourmaline crystals exhibited pyroelectricity and piezoelectricity.
또한, 바이오스톤(biostone)은 인체 동물 및 식물의 생명유지에 요구되는 천연 미량원소 미네랄로 구성된 물질로서, 종래에 무독한 가축 보조사료로 사용되어 왔다. 바이오스톤의 pH는 9.3으로 알칼리성을 나타내며, 비표면적 7.9㎡/g인 다공질 천연 미네랄로서 흡착 탈취효과가 크다.In addition, biostone (biostone) is a material composed of natural trace element minerals required for the maintenance of human animal and plant life, has been used as a conventional livestock supplement feed. Biostone has a pH of 9.3, which shows alkalinity, and is a porous natural mineral having a specific surface area of 7.9 m 2 / g.
한편, 종래의 증수개량제는 농축된 고밀도의 액상형태로서 제조되었다. 이들의 증수효과는 어느 정도 있지만, 가격이 비교적 고가로서 재배된 버섯의 가격이 증가되어 경쟁력이 떨어지는 문제점이 있다.On the other hand, conventional thickener was prepared as a concentrated high density liquid form. Although there is some increase effect of these, the price is relatively high, there is a problem that the competitiveness of the cultivated mushrooms is increased as the price is increased.
따라서, 종래의 값비싼 버섯 증수개량제를 대체하면서, 버섯균사와 자실체의 빠른 성장 및 버섯의 증수를 이룰 수 있는 신규한 버섯 증수개량제의 개발이 당업계에서 요구되었다.Therefore, there is a need in the art for the development of a novel mushroom multiplier that can achieve rapid growth of mushroom mycelium and fruiting bodies and multiplication of mushrooms, while replacing conventional expensive mushroom multipliers.
이에, 본 발명자들은 고품질의 버섯을 저비용으로 다수확하기 위한 방법을찾고자 연구를 수행한 결과, 옥수수 배아박, 농축된 당밀의 가용성 발효물질(condensed molasses fermentation solubles; 이하, 'CMS'라 함), 바이오스톤, 전기석(tourmaline), 활성탄, 황산아연을 포함하는 조성물이 버섯 증수개량제로 사용함으로써, 고품질의 버섯을 저비용으로 다수확할 수 있음을 발견하고 본 발명을 완성하기에 이르렀다.Therefore, the present inventors conducted a study to find a method for obtaining a high-quality mushroom at a low cost and large numbers, and as a result, corn embryos, condensed molasses fermentation solubles (hereinafter referred to as 'CMS'), bio The composition comprising a stone, tourmaline, activated carbon, and zinc sulfate has been found to be able to obtain a large number of high-quality mushrooms at low cost by completing the present invention.
따라서, 본 발명의 목적은 고품질의 버섯을 저비용으로 다수확하기 위한 버섯 증수개량제 조성물을 제공하는 것이다.Accordingly, it is an object of the present invention to provide a mushroom multiplier composition for harvesting high quality mushrooms at low cost.
상기한 목적을 달성하기 위해서, 본 발명에 따른 조성물은 조성물 총 중량에 대하여 옥수수 배아박 40∼80중량%, CMS 10∼30중량%, 바이오스톤 5∼25중량%, 전기석 1∼5중량%, 활성탄 1∼5중량% 및 황산아연 0.1∼2중량%를 함유함을 특징으로 한다.In order to achieve the above object, the composition according to the present invention is based on the total weight of the composition 40 to 80% by weight of corn germ, 10 to 30% by weight CMS, 5 to 25% by weight biostone, 1 to 5% by weight tourmaline, 1 to 5% by weight of activated carbon and 0.1 to 2% by weight of zinc sulfate.
또한, 본 발명에 따른 증수 개량제 조성물은, 톱밥과 미강(일명, 쌀겨)으로 이루어진 종래의 버섯 배양배지 중에서, 미강을 대체하여 버섯 배양배지 총 부피에 대하여 5∼25%(v/v)의 비율로 첨가되는 것이 바람직하다. 이렇게 제조된 버섯 배양배지는 증수 효과와 배지의 물리성이 개선될 수 있다. 본 발명에 따르면, 버섯 배양배지에 증수개량제 조성물을 5%(v/v) 미만으로 첨가하거나, 25%(v/v)를 초과하여 사용하는 경우, 버섯 균사의 생장속도가 느려지고, 균사의 밀도가 낮아져 고품질의 버섯을 재배할 수 없으므로, 바람직하지 않다.In addition, the increase-improvement composition according to the present invention, in the conventional mushroom culture medium consisting of sawdust and rice bran (aka, rice bran), replacing the rice bran to a ratio of 5 to 25% (v / v) to the total volume of the mushroom culture medium Is preferably added. Mushroom culture medium thus prepared can be improved in the effect of increasing the physical properties of the medium. According to the present invention, when the increase-improvement agent composition is added to the mushroom culture medium in less than 5% (v / v), or when used in excess of 25% (v / v), the growth rate of the mushroom mycelia is slow, the density of the mycelia It is not desirable to lower the quality of mushrooms grown.
한편, 본 발명에 따른 조성물은 증수개량제 조성물 총 중량에 대하여 옥수수배아박을 40∼80중량%의 범위 내로 함유한다. 옥수수 배아박은 버섯 배양배지에 풍부한 영양물질을 제공하며, 또한, 비타민 중 바이오틴과 티아민(thiamine) 성분이 풍부하여 균사생장 촉진인자로 작용한다. 따라서, 옥수수 배아박을 상기한 범위 이하로 사용할 경우, 균사 생장이 촉진되지 않으며 균사의 밀도 또한 낮아져서, 고품질의 버섯을 얻을 수 없게 된다.On the other hand, the composition according to the present invention is corn germ It contains in 40 to 80weight% of a range. Corn embryos provide abundant nutrients to the mushroom culture medium, and also act as a mycelial growth promoter due to the abundance of biotin and thiamine in vitamins. Therefore, when corn germ foil is used below the above-mentioned range, mycelial growth is not promoted and the density of the mycelium is also lowered, and a high quality mushroom cannot be obtained.
또한, 본 발명에 따른 조성물은 조성물 총 중량에 대하여 CMS를 10∼30중량%의 범위로 함유한다. CMS는 씨에이엠피(c-amp)라고 하는 자실체 형성유도물질이 함유되어 있어, 버섯발이 개체수를 많게 하여 수량을 증수토록 유도한다. 따라서, CMS를 상기 범위 이하의 양으로 사용하는 경우, 버섯증수 효과가 낮아지므로, 바람직하지 않다. 한편, CMS의 첨가량은 옥수수 배아박의 양을 고려하여 적절하게 선택할 수 있다.In addition, the composition according to the present invention contains the CMS in the range of 10 to 30% by weight based on the total weight of the composition. CMS contains fruiting body-forming substance called c-amp, which induces abundant mushroom shoots to increase the yield. Therefore, when the CMS is used in an amount below the above range, the mushroom water evaporation effect is lowered, which is not preferable. On the other hand, the addition amount of CMS can be suitably selected in consideration of the quantity of corn germ foil.
또한, 본 발명에 따른 조성물은 조성물 총 중량에 대하여 바이오스톤을 5∼25중량%의 범위로 함유한다. 바이오스톤은 원적외선을 방산하여 자실체 발이와 생육을 촉진시키며, 배지의 영양원소를 균형있게 흡수하도록 배지의 산도변화에 대한 완충작용을 하여, 균사의 초기생육을 왕성하게 한다. 따라서, 본 발명에 따른 조성물은 바이오스톤을 함유함으로써, 균사의 생장속도 및 균사 밀도를 증가시킬 수 있고, 다른 잡균 보다 버섯균사의 생장속도를 우점하도록 유도하여, 버섯 균사의 병해충 감염에 내성을 갖도록 할 수 있다. 한편, 바이오스톤을 상기한 범위를 벗어난 양으로 사용하는 경우, 균사의 생장속도 및 균사 밀도를 증가효과를 얻을 수 없어 바람직하지 않다.In addition, the composition according to the present invention contains the biostone in the range of 5 to 25% by weight based on the total weight of the composition. Biostone dissipates far-infrared rays, promotes fruiting and growth, and buffers the acidity change of the medium to balance the nutrients of the medium. Therefore, the composition according to the present invention, by containing a biostone, can increase the growth rate and mycelial density of mycelia, and induces the growth rate of mushroom mycelium more dominant than other various bacteria, so as to be resistant to pest infection of mushroom mycelia. can do. On the other hand, when the biostone is used in an amount outside the above range, it is not preferable because the effect of increasing the growth rate and the mycelial density of mycelia cannot be obtained.
또한, 본 발명에 따른 조성물은 조성물 총 중량에 대하여, 전기석을 1∼5중량%의 범위로 함유하는 것이 바람직하다. 전기석은 버섯 영양원의 미량원소인 무기염류를 배양배지에 용해되기 쉽도록 하여, 균사생장과 고품질의 버섯 수량을 촉진한다. 한편, 전기석을 상기한 범위를 벗어난 양으로 사용하는 경우, 전기석의 상기 효과를 얻어낼 수 없어 바람직하지 않다.In addition, the composition according to the present invention preferably contains a tourmaline in the range of 1 to 5% by weight based on the total weight of the composition. Tourmaline facilitates dissolution of inorganic salts, which are trace elements of mushroom nutrients, into culture media, promoting mycelial growth and high-quality mushroom yields. On the other hand, when the tourmaline is used in an amount outside the above range, the above effects of the tourmaline cannot be obtained, which is not preferable.
또한, 본 발명에 따른 조성물은 버섯균사 생장시 발생하는 유해가스를 포집하여 균사가 잘 자라도록 호기성 조건을 유지시켜 주기 위해서, 조성물 총 중량에 대하여 활성탄을 1∼5중량%의 범위로 함유한다.In addition, the composition according to the present invention contains activated carbon in the range of 1 to 5% by weight based on the total weight of the composition in order to collect a harmful gas generated during mushroom mycelium to maintain aerobic conditions so that the mycelia grow well.
또한, 본 발명에 따른 조성물은 세균성 갈반병균 및 푸른 곰팡이균의 길항작용을 유도함으로서 버섯균사의 내병성을 증진시키기 위하여, 조성물 총 중량에 대하여 황산아연을 0.1∼2중량%의 범위로 함유한다.In addition, the composition according to the present invention contains zinc sulfate in the range of 0.1 to 2% by weight based on the total weight of the composition in order to enhance the pathogenicity of the mushroom mycelia by inducing antagonism of bacterial gallbladder and blue fungus.
따라서, 상기 옥수수 배아박, CMS, 바이오스톤, 전기석, 활성탄, 황산아연을 포함하는 버섯 증수개량제 조성물은, 버섯 균사의 생장속도 및 생장밀도를 증가시키고, 버섯 증수를 개선할 뿐만 아니라, 병해충에 대한 내성도 얻어낼 수 있으므로, 고품질의 버섯을 다량으로 생산할 수 있다. 또한, 본 발명에 따른 조성물은 버섯배지에 버섯균사와 자실체가 생장하는데 필요한 필수영양소(13가지 : C, H, O, N, P, K, B, Mn, Cu, Zn. Mo, S, Mg, Fe)와 선택영양소(Ca) 및 생장촉진인자로서 비타민의 일종인 바이오틴(biotin) 또는 티아민(thiamine)를 고루 갖춘 배지를 값싸게 제공할 수 있어, 고품질의 버섯을 값싸게 생산할 수 있고, 농가소득을 증가시킬 수 있다.Accordingly, the mushroom thickener composition comprising the corn germ meal, CMS, biostone, tourmaline, activated carbon, zinc sulfate, not only increases the growth rate and growth density of mushroom mycelia, but also improves mushroom yield, Since resistance can also be obtained, high-quality mushrooms can be produced in large quantities. In addition, the composition according to the present invention is essential nutrients (13 kinds: C, H, O, N, P, K, B, Mn, Cu, Zn.Mo, S, Mg) required for the growth of mushroom mycelium and fruiting bodies in mushroom medium , Fe), selective nutrients (Ca), and growth factors, such as biotin or thiamine, which is a kind of vitamin, can be provided inexpensively, so that high-quality mushrooms can be produced cheaply, and farms You can increase your income.
이하, 하기 참고 실시예 및 실시예를 통하여 본 발명을 더욱 구체적으로 설명하지만, 본 발명이 이에 의해서 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail with reference to the following Examples and Examples, but the present invention is not limited thereto.
[참고 실시예1] 버섯 균사체 및 접종원의 제조Reference Example 1 Preparation of Mushroom Mycelia and Inoculum
하기 실시예에서는, 한국농업전문학교에 보관중인 느타리버섯균(Pleurotus ostreatusKNAC1002), 큰느타리버섯균(Pleurotus eryngiiKNAC1043), 팽이버섯균(Flamulina velutipesKNAC1402), 표고버섯균(Lentinus edodesKNAC1109), 영지버섯균(Ganoderma lucidumKNAC2502)의 버섯균을 이용하여 실험을 수행하였다.In the following examples, Pleurotus ostreatus KNAC1002, Pleurotus eryngii KNAC1043, Flamulina velutipes KNAC1402, Shiitake fungus ( Lentinus edodes ) The experiment was performed using the mushroom bacteria of Ganoderma lucidum KNAC2502.
또한, 톱밥과 쌀겨를 80:20(v/v)의 비율로 혼합한 다음, 배양배지의 총 건조중량에 대하여 70중량%의 수분을 첨가하여 제조한 다음, 고압 살균하여 접종원을 얻기 위한 버섯균사 배양배지를 제조하였다.In addition, after mixing the sawdust and rice bran at a ratio of 80:20 (v / v), and then prepared by adding 70% by weight of water to the total dry weight of the culture medium, mushroom hyphae to obtain inoculum by autoclaving Culture medium was prepared.
버섯 균사 접종원은, 상기에서 제조한 버섯 균사 배양배지 100g을 함유하고 있는 250㎖ 삼각플라스크에, PDA(potato dextrose agar) 배지에서 10일간 배양한 상기 버섯균 각각의 균사절편(2×3㎝)을 두 개씩 무균접종한 다음, 8일간 더 배양하여 획득하였다.Mushroom mycelia inoculum is a mycelium section (2 x 3 cm) of each of the mushroom bacteria cultured in a PDA (potato dextrose agar) medium for 10 days in a 250 ml Erlenmeyer flask containing 100 g of the mushroom mycelia culture medium prepared above. After aseptic inoculation two by two, it was obtained by further incubation for 8 days.
[참고 실시예 2] 옥수수 배아박 및 CMS의 구입Reference Example 2 Purchasing Corn Germ Park and CMS
하기 실험에서 이용한 옥수수 배아박 및 CMS는, (주)경기유지에서 옥수수유를 생산하고 남은 옥수수 배아 찌꺼기로부터 얻었다.Corn germ foil and CMS used in the following experiment were obtained from corn germ residues left after producing corn oil in Gyeonggi Co., Ltd.
[참고 실시예 3] 버섯 균사별 배지의 제조[Reference Example 3] Preparation of mushroom mycelia
하기의 실험예에서, 버섯 균사별로 톱밥 종류를 달리 사용하여, 공시균의 균사생장과 밀도에 적합한 조건을 조성하였다. 느타리버섯과 큰느타리버섯은 이태리 포플러톱밥(Populus euramericama)을 사용하였고, 팽이버섯은 미송톱밥(Pinus densiflora)을 사용하였으며, 표고 및 영지버섯은 졸참나무톱밥(Quercus serrata)을 사용하였다.In the following experimental example, by using different types of sawdust for each mushroom mycelia, conditions suitable for mycelial growth and density of the test bacteria were formed. Pleurotus eryngii and Pleurotus eryngii used Italian Populus euramericama , Pinus densiflora for Enoki mushroom, Quercus serrata for Shiitake and Ganoderma lucidum.
[실시예 1] 증수개량제의 제조Example 1 Preparation of Increased Enhancer
하기 표 1에 나타낸 비율로 각각의 성분들을 혼합하여, 증수개량제 혼합물을 제조하였다.Each of the components was mixed in the proportions shown in Table 1 below to produce a multimodal modifier mixture.
상기의 혼합물을 참나무 목초액 200배액으로, 수분이 약 40%가 되도록 혼합한 다음, 이를 수분함량이 10% 이하가 되도록 다시 건조시켜 증수개량제를 제조하였다.The above mixture was mixed with 200 times the oak vinegar solution, so that the water content was about 40%, and then dried again so that the water content was 10% or less to prepare a thickener.
[실험예 1] 느타리버섯 균사생장, 밀도 및 수량에 미치는 증수개량제의 영향[Experimental Example 1] Effect of the increase agent on mycelial growth, density and yield
실시예 1에서 제조된 증수개량제를 배양배지 총 부피에 대하여 0, 5, 10, 15, 20 및 25%(v/v)의 양으로 이태리 포플러 톱밥에 첨가하여 느타리버섯용 톱밥 배양배지를 만들고, 이를 시험관(3.0×20.0cm)에 50g(가비중 0.21)씩 일정하게 충진한 후, 121℃에서 30분간 고압살균한 다음, 상기 참고 실시예 1의 방법으로 제조한 느타리버섯 접종원을 3∼5g씩 접종하였다. 그런 다음, 균사의 밀도 및 5일 간격으로 생장한 균사의 길이를 측정하였다. 또한, 대조군은 증수개량제가 첨가되지 않은 것으로 하였다. 그 결과를 하기 표 2에 나타내었다.The increase-improving agent prepared in Example 1 was added to Italian poplar sawdust in an amount of 0, 5, 10, 15, 20 and 25% (v / v) based on the total volume of the culture medium to make sawdust culture medium for oyster mushroom, After filling the test tube (3.0 × 20.0cm) by 50g (specific weight 0.21), and then sterilized under high pressure at 121 ℃ for 30 minutes, 3 to 5g each of the oyster mushroom inoculum prepared by the method of Reference Example 1 Inoculation. Then, the density of the hyphae and the length of the hyphae grown at 5-day intervals were measured. In addition, the control group was supposed that no increase agent was added. The results are shown in Table 2 below.
느타리버섯균사는, 증수개량제를 15%로 첨가하였을 때, 균사생장속도가 117mm/10일로 대조군 74mm/10일보다 현저히 높았고 균사밀도도 높았다. 수량은 대조군 96g에 비하여, 114g으로 18.8%가 증수되었다.The mycelial growth of mycelial growth was 117 mm / 10 days when the increase agent was added at 15%, which was significantly higher than the control group 74 mm / 10 days and the mycelial density was high. Yield was increased 18.8% to 114g compared to 96g control.
[실험예 2] 큰느타리버섯 균사생장, 밀도 및 수량에 미치는 증수개량제의 영향[Experimental Example 2] Effect of the increase agent on mycelial growth, density and yield
실시예 1에서 제조된 증수개량제를 배양배지 총 부피에 대하여 0, 5, 10, 15, 20 및 25%(v/v)의 양으로 이태리 포플러 톱밥에 첨가하여 큰느타리버섯용 톱밥 배양배지를 만들고, 이를 시험관(3.0×20.0cm)에 50g(가비중 0.21)씩 일정하게 충진한 후, 121℃에서 30분간 고압살균한 다음, 상기 참고 실시예 1의 방법으로 제조한 큰느타리버섯 접종원을 3∼5g씩 접종하였다. 그런 다음, 균사의 밀도 및 5일 간격으로 생장한 균사의 길이를 측정하였다. 또한, 대조군은 증수개량제가 첨가되지 않은 것으로 하였다. 그 결과를 하기 표 3에 나타내었다.The multiplication agent prepared in Example 1 was added to Italian poplar sawdust in an amount of 0, 5, 10, 15, 20 and 25% (v / v) based on the total volume of the culture medium to make sawdust culture medium for mushrooms After filling the test tube (3.0 × 20.0cm) with 50g (specific gravity 0.21) at a constant rate, sterilizing at high pressure at 121 ° C. for 30 minutes, and then preparing 3 to 2 large mushrooms inoculated by the method of Reference Example 1 above. 5 g each was inoculated. Then, the density of the hyphae and the length of the hyphae grown at 5-day intervals were measured. In addition, the control group was supposed that no increase agent was added. The results are shown in Table 3 below.
큰느타리버섯(일명 새송이) 균사는 증수개량제를 20%로 첨가하였을 때, 균사생장속도가 120mm/10일로, 대조군 78mm/10일보다 현저히 높았고, 균사밀도도 높았다. 수량은 대조군 84g에 비하여 102g으로 21.4%가 증수되었다.The mycelial growth of myrtle (also known as Pleurotus eryngii) was 120 mm / 10 days, which was significantly higher than that of the control group 78 mm / 10 days, and the mycelial density was also higher when the increase agent was added at 20%. Yield was increased by 21.4% to 102g compared to 84g control.
[실험예 3] 팽이버섯 균사생장, 밀도 및 수량에 미치는 증수개량제의 영향[Experimental Example 3] Effect of the increase agent on the mycelial growth, density and yield of the enoki mushroom
실시예 1에서 제조된 증수개량제를 배양배지 총 부피에 대하여 0, 5, 10, 15, 20 및 25%(v/v)의 양으로 이태리 미송 톱밥에 첨가하여 팽이버섯용 톱밥 배양배지를 만들고, 이를 시험관(3.0×20.0cm)에 50g(가비중 0.21)씩 일정하게 충진한 후, 121℃에서 30분간 고압살균한 다음, 상기 참고 실시예 1의 방법으로 제조한 팽이버섯 접종원을 3∼5g씩 접종하였다. 그런 다음, 균사의 밀도 및 5일 간격으로 생장한 균사의 길이를 측정하였다. 또한, 대조군은 증수개량제가 첨가되지 않은 것으로 하였다. 그 결과를 하기 표 4에 나타내었다.The increase-improving agent prepared in Example 1 was added to Italian rice chopped sawdust in an amount of 0, 5, 10, 15, 20 and 25% (v / v) based on the total volume of the culture medium to make a sawdust culture medium for the mushrooms, After filling the test tube (3.0 × 20.0cm) by 50g (specific weight 0.21), and then sterilized under high pressure at 121 ℃ for 30 minutes, 3 to 5g each of the oyster mushroom inoculation prepared by the method of Reference Example 1 Inoculation. Then, the density of the hyphae and the length of the hyphae grown at 5-day intervals were measured. In addition, the control group was supposed that no increase agent was added. The results are shown in Table 4 below.
팽이버섯 균사는, 증수개량제를 15%로 첨가하였을 때, 균사생장속도가116mm/10일로 대조군 70mm/10일보다 현저히 높았고 균사밀도도 높았다. 수량은 대조군 171g에 비하여 197g으로 15.2%가 증수되었다.The fungus mycelium had a mycelial growth rate of 116 mm / 10 days, which was significantly higher than that of the control group 70 mm / 10 days, and the mycelial density was also higher when 15% of increase was added. The yield was increased by 15.2% to 197g compared to 171g of the control group.
[실험예 4] 표고버섯 균사생장, 밀도 및 수량에 미치는 증수개량제의 영향[Experimental Example 4] Effect of the increase agent on shiitake mushroom mycelial growth, density and yield
실시예 1에서 제조된 증수개량제를 배양배지 총 부피에 대하여 0, 5, 10, 15, 20 및 25%(v/v)의 양으로 졸참나무 톱밥에 첨가하여 표고버섯용 톱밥 배양배지를 만들고, 이를 시험관(3.0×20.0cm)에 50g(가비중 0.21)씩 일정하게 충진한 후, 121℃에서 30분간 고압살균한 다음, 상기 참고 실시예 1의 방법으로 제조한 표고버섯 접종원을 3∼5g씩 접종하였다. 그런 다음, 균사의 밀도 및 5일 간격으로 생장한 균사의 길이를 측정하였다. 또한, 대조군은 증수개량제가 첨가되지 않은 것으로 하였다. 그 결과를 하기 표 5에 나타내었다.To increase the amount of 0, 5, 10, 15, 20 and 25% (v / v) of the hydration oak sawdust culture medium for the shiitake mushroom sawdust culture medium prepared in Example 1, After filling the test tube (3.0 × 20.0cm) by 50g (specific weight 0.21), and then sterilized under high pressure at 121 ℃ for 30 minutes, 3 to 5g of shiitake inoculum prepared by the method of Reference Example 1 Inoculation. Then, the density of the hyphae and the length of the hyphae grown at 5-day intervals were measured. In addition, the control group was supposed that no increase agent was added. The results are shown in Table 5 below.
표고버섯 균사는, 증수개량제를 20%로 첨가하였을 때, 균사 생장속도가 105mm/10일로 대조군 75mm/10일보다 현저히 높았고 균사밀도도 높았다. 수량은 대조군 84g에 비하여 109g으로 29.8%가 증수되었다.Shiitake mycelium had a mycelial growth rate of 105 mm / 10 days, which was significantly higher than that of the control group 75 mm / 10 days and high mycelial density when added with a 20% increase in modifier. Yield was increased 29.8% to 109g compared to 84g of the control group.
[실험예 5] 영지버섯 균사생장, 밀도 및 수량에 미치는 증수개량제의 영향[Experiment 5] Effect of the increase agent on the mycelial growth, density and yield of Ganoderma lucidum
실시예 1에서 제조된 증수개량제를 배양배지 총 부피에 대하여 0, 5, 10, 15, 20 및 25%(v/v)의 양으로 졸참나무 톱밥에 첨가하여 영지버섯용 톱밥 배양배지를 만들고, 이를 시험관(3.0×20.0cm)에 50g(가비중 0.21)씩 일정하게 충진한 후, 121℃에서 30분간 고압살균한 다음, 상기 참고 실시예 1의 방법으로 제조한 영지버섯 접종원을 3∼5g씩 접종하였다. 그런 다음, 균사의 밀도 및 5일 간격으로 생장한 균사의 길이를 측정하였다. 또한, 대조군은 증수개량제가 첨가되지 않은 것으로 하였다. 그 결과를 하기 표 6에 나타내었다.To increase the amount of 0, 5, 10, 15, 20 and 25% (v / v) to the camphor oak sawdust culture medium prepared in Example 1 to make a sawdust culture medium for Ganoderma lucidum mushroom, After filling the test tube (3.0 × 20.0cm) by 50g (specific weight 0.21), and then autoclaved at 121 ℃ for 30 minutes, 3 to 5g each of Ganoderma lucidum inoculation prepared by the method of Reference Example 1 Inoculation. Then, the density of the hyphae and the length of the hyphae grown at 5-day intervals were measured. In addition, the control group was supposed that no increase agent was added. The results are shown in Table 6 below.
영지버섯 균사는, 증수개량제를 15%로 첨가하였을 때, 균사생장 속도가 119mm/10일로 대조군 88mm/10일보다 현저히 높았고 균사밀도도 높았다. 수량은 대조군 79g에 비하여 94g으로 약 20%가 증수되었다.Ganoderma lucidum mycelium had a mycelial growth rate of 119 mm / 10 days, which was significantly higher than that of the control group 88 mm / 10 days, and mycelial density was also higher when 15% of increase was added. The yield was increased by about 20% to 94 g compared to 79 g of the control group.
이상에서 살펴본 바와 같이, 본 발명에 따른 조성물은 느타리버섯, 큰느타리버섯, 팽이버섯, 표고버섯 및 영지버섯 등의 버섯균사를 고밀도로 빠르게 성장시킬 뿐만 아니라, 버섯 증수에도 현저한 효과가 있음을 알 수 있다. 따라서, 본 발명에 따른 조성물은 다양한 버섯의 증수 개량제로서 이용될 수 있다.As described above, the composition according to the present invention not only rapidly grow mushroom hyphae, such as oyster mushroom, large oyster mushroom, enoki mushroom, shiitake mushroom and ganoderma lucidum at high density, but also has a noticeable effect on mushroom increase. have. Therefore, the composition according to the present invention can be used as a thickener for various mushrooms.
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