KR100398677B1 - Cultivation Method of mushroom mycelium using citrus juice and mushroom mycelium thereof - Google Patents
Cultivation Method of mushroom mycelium using citrus juice and mushroom mycelium thereof Download PDFInfo
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- KR100398677B1 KR100398677B1 KR10-2001-0049267A KR20010049267A KR100398677B1 KR 100398677 B1 KR100398677 B1 KR 100398677B1 KR 20010049267 A KR20010049267 A KR 20010049267A KR 100398677 B1 KR100398677 B1 KR 100398677B1
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Abstract
본 발명은 감귤농축액을 배지로 이용하여 짧은 기간에 대량의 버섯균사체 및 유용한 성분을 함유하는 버섯균사체 배양물을 얻는 버섯균사체의 배양방법에 관한 것으로 특히, 비 상품성 감귤 농축액에 버섯균사체를 접종하여 증식배양으로 대량의 버섯균사체와 그 배양액을 획득하여 단백다당체를 생산하거나 기능성식품소재 및 기능성물질, 양식어류 그리고 동물사료 첨가제 등의 소재로서 대량으로 공급하고자 하는 것이다.The present invention relates to a method for culturing mushroom mycelium, which obtains a mushroom mycelium culture containing a large amount of mushroom mycelium and useful components in a short period of time using a citrus concentrate as a medium, and particularly, inoculates mushroom mycelium to a non-commercial citrus concentrate. It is intended to obtain a large amount of mushroom mycelium and its culture by cultivation to produce protein polysaccharides or to supply a large amount as functional food materials and functional materials, farmed fish and animal feed additives.
본 발명의 버섯균사체 배양방법은, 버섯균사체의 액체배양에 있어서, 균주로서, 버섯균사체를 사용하고, 감귤농축액을 배지로 하여 약 121℃에서 15~20분간 고압습윤 멸균한 후 버섯종균을 접종하여 증식배양으로 대량의 버섯균사체와 그 배양액을 획득하는 것을 특징으로 하고, 그 버섯균사체는 감귤농축액으로부터 액체 배양된 버섯균사체를 약 85℃의 뜨거운 물로 약 24시간 추출한 후 에탄올로 침전 시키고, 이를 동결 건조한 것을 특징으로 한다. 이에 따라 상황버섯 균사체, 낙옆송층버섯 균사체, 영지버섯 균사체, 표고버섯 균사체, 운지버섯 균사체 등의 배양에 있어서 특별한 첨가물이 없이도 대량생산이 가능하고, 대량 배양된 버섯균사체를 버섯자실체 형성을 위한 종균으로 이용할 수 있으며, 비교적 짧은 기간에 버섯균사체를 대량으로 배양할 수 있고, 그 배양으로부터 기능성 물질을 추출할 수 있으며, 버섯균사체의 배양에 따른 대사산물의 추출과 균사체의 회수에 있어서 저비용 고효율화가 가능하다.Mushroom mycelium culturing method of the present invention, in liquid culture of the mushroom mycelium, mushroom mycelium as a strain, using a citrus concentrate as a medium to autoclave for 15-20 minutes at 121 ℃ inoculated mushroom seedlings It is characterized by obtaining a large number of mushroom mycelium and its culture solution by propagation culture, the mushroom mycelium is extracted from the citrus concentrate liquid cultured mushroom mycelium with hot water of about 85 ℃ for about 24 hours and then precipitated with ethanol, and freeze-dried It is characterized by. Therefore, it is possible to mass-produce without special additives in cultivation of situation mushroom mycelium, Lactobacillus fungus mycelium, Ganoderma lucidum mycelium, shiitake mushroom mycelium, and fingering mushroom mycelium, and the mass cultured mushroom mycelium is used as a spawn for forming the mushroom fruit body. It can be used, it is possible to cultivate mushroom mycelium in a large amount in a relatively short period of time, and to extract the functional material from the culture, it is possible to lower the cost and high efficiency in extracting the metabolite and recovery of the mycelium by culturing the mushroom mycelium. .
Description
본 발명은 감귤농축액을 배지로 이용하여 짧은 기간에 대량의 버섯균사체 및 유용한 성분을 함유하는 버섯균사체 배양물을 얻는 버섯균사체의 배양방법에 관한 것으로 특히, 비 상품성 감귤 농축액에 버섯균사체를 접종하여 증식배양으로 대량의 버섯균사체와 그 배양액을 획득하여 단백다당체를 생산하거나 기능성식품소재 및 기능성물질, 양식어류 그리고 동물사료 첨가제 등의 소재로서 대량으로 공급하고자 하는 것이다.The present invention relates to a method for culturing mushroom mycelium, which obtains a mushroom mycelium culture containing a large amount of mushroom mycelium and useful components in a short period of time using a citrus concentrate as a medium, and particularly, inoculates mushroom mycelium to a non-commercial citrus concentrate. It is intended to obtain a large amount of mushroom mycelium and its culture by cultivation to produce protein polysaccharides or to supply a large amount as functional food materials and functional materials, farmed fish and animal feed additives.
일반적으로 버섯균사체를 배양하는 기존방법으로는 고체 배지에 의한 방법과 액체배지에 의한 방법으로 나눌 수 있다.In general, the conventional method of culturing mushroom mycelium can be divided into a method by a solid medium and a liquid medium.
버섯균사체의 배양을 위한 전자의 고체 배지 방법은, 배양에 주로 사용되는 배지로서 밀, 보리, 감자 등이 있으나 이들 원료의 특성상 배양이 완료된 후 균사체만을 회수하는 작업이 자동화되기 어렵기 때문에 비용이 많이 드는 단점이 있다. 또한 버섯균사체가 성장하면서 만들어내는 유용한 대사산물을 추출하는데도 어려움이 크다.The former solid medium method for the cultivation of mushroom mycelium is a medium mainly used for cultivation, but wheat, barley, potato, etc., but due to the characteristics of these raw materials, it is difficult to recover only the mycelium after cultivation is completed, which is expensive. There is a disadvantage. It is also difficult to extract useful metabolites that mushroom mycelium grows.
버섯균사체의 배양을 위해 이용되는 또 다른 액체 배지 방법의 경우는 균사체만을 얻기 위해 인위적으로 만든 배지를 이용하거나 식품부산물을 이용하기도 하는데 인위적 배지의 경우 가격이 비교적 고가이며, 식품 부산물의 경우 일정한 성분을 확신할 수 없어 균일한 균사체나 혹은 대사산물을 얻기에는 무리가 있다. 또한 식품부산물 등은 안정성을 보장할 수 없기 때문에 그 배양액을 기능성 식품 등으로 이용하기에는 이용성의 한계를 가지게 된다.Another liquid medium method used for the cultivation of mushroom mycelium is to use artificially made medium or food by-products to obtain only the mycelium. I'm not sure it's hard to get a uniform mycelium or metabolite. In addition, since food by-products and the like cannot be guaranteed, the culture solution has a limit in usability for use as a functional food.
이와 같이 버섯균사체를 배양하는 종래의 배지 방법들을 크게 구분하면 고체/액체 배지 방법으로 구분할 수 있으나, 고비용성과 유용한 대사산물 추출의 한계, 그리고 안정성 등이 부족한 문제점이 있었다.As such, the conventional medium methods for culturing mushroom mycelium can be largely classified into solid / liquid medium methods, but there is a problem of lack of high cost, useful metabolite extraction, and stability.
따라서 본 발명의 목적은 비교적 짧은 기간에 버섯균사체를 대량으로 배양할 수 있으며, 그 배양으로부터 기능성 물질을 추출할 수 있게 하는 버섯균사체의 배양방법을 제공하는 것이다.Accordingly, it is an object of the present invention to provide a method for culturing mushroom mycelium, which is capable of cultivating a large amount of mushroom mycelium in a relatively short period of time, and extracting a functional substance from the culture.
본 발명의 다른 목적은 버섯균사체 배양에 있어서 안정성이 보장된 배지를 이용하여 그 배양액을 기능성 식품 원료, 양식어류 및 동물사료 첨가재 등으로 공급하기 위한 것이다.Another object of the present invention is to supply the culture solution as a functional food raw material, aquaculture fish and animal feed additives, etc. using a medium having a stable stability in mushroom mycelium culture.
본 발명의 또 다른 목적은 버섯균사체 배양을 위한 배지로서 감귤농축액을 이용함으로서 비상품성 감귤의 이용성을 다양화시키는 것이다.Another object of the present invention is to diversify the availability of non-commercial citrus fruits by using citrus concentrate as a medium for mushroom mycelium culture.
본 발명의 또 다른 목적은 버섯균사체의 배양에 따른 대사산물의 추출과 균사체의 회수에 있어서 저비용 고효율화가 가능한 버섯균사체의 배양방법을 제공하는 것이다.Still another object of the present invention is to provide a method for culturing mushroom mycelium which is capable of low cost and high efficiency in extracting metabolites and recovering mycelium according to the culture of mushroom mycelium.
이러한 목적을 달성하기 위한 본 발명의 버섯균사체 배양방법은,Mushroom mycelium culture method of the present invention for achieving this object,
버섯균사체의 액체배양에 있어서,In liquid culture of mushroom mycelium,
균주로서, 버섯균사체를 사용하고, 감귤농축액을 배지로 하여 약 121℃에서 15~20분간 고압습윤 멸균한 후 버섯종균을 접종하여 증식배양으로 대량의 버섯균사체와 그 배양액을 획득하는 것을 특징으로 한다.As a strain, mushroom mycelium is used, and after the high-pressure wet sterilization at about 121 ℃ for 15-20 minutes using a citrus concentrate as a medium, and inoculated mushroom seedlings to obtain a large number of mushroom mycelium and its culture by growth culture. .
본 발명의 다른 특징은 감귤농축액으로부터 액체 배양된 버섯균사체를 약 85℃의 뜨거운 물로 약 24시간 추출한 후 에탄올로 침전 시키고, 동결건조한 감귤농축액을 이용하여 배양된 버섯균사체를 특징으로 한다.Another feature of the present invention is characterized in that the mushroom mycelium liquid cultured from the citrus concentrate is extracted with hot water at about 85 ℃ for about 24 hours to precipitate with ethanol, and cultured mushroom mycelium using lyophilized citrus concentrate.
도 1은 본 발명의 버섯균사체 배양방법에 따른 발효기록표1 is a table of fermentation records according to the mushroom mycelium culture method of the present invention
도 2는 본 발명에 따라 수득한 버섯균사체 배양액의 혈액종양세포(HL-60)에 대한 세포억제율표Figure 2 is a cell inhibition rate table for blood tumor cells (HL-60) of the mushroom mycelium culture broth obtained according to the present invention
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본 발명은 버섯균사체의 액체배양과 관련된다. 본 발명은 지속적으로생산 가능한 감귤농축액을 이용하여 비교적 짧은 기간에 버섯균사체를 대량으로 배양하여 기능성 물질을 추출하는 것이다. 또한 본 발명은 안정성이 검증되고 보장된 식용 가능한 감출농축액을 배지로 이용하여 배양액과 균사체를 동시에 획득하여 간 기능 개선, 항암 및 면역계의 활성을 증가시키는 다당체를 생산해내거나, 기능성 식품 소재 및 기능성 물질, 양식어류 및 동물사료 첨가재 소재로서 대량으로 공급될 수 있는 버섯균사체의 배양방법으로서, 감귤농축액을 배지로 하여 121℃에서 15~20분간 멸균한 후 버섯종균을 접종하여 약 7일간의 증식배양으로 대량의 버섯균사체와 그 배양액을 획득한다.The present invention relates to the liquid culture of mushroom mycelium. The present invention is to extract a functional material by culturing a large amount of mushroom mycelium in a relatively short period using a continuously produced citrus concentrate. In another aspect, the present invention is to obtain a culture and mycelium at the same time using the edible sensitized concentrate, which has been verified and guaranteed stability to produce a polysaccharide to improve liver function, increase the activity of anti-cancer and immune system, or functional food material and functional material, As a method of culturing mushroom mycelium which can be supplied in large quantities as a raw material for farmed fish and animal feed, sterilize 15 to 20 minutes at 121 ° C. with citrus concentrate as a medium, and inoculate mushroom seedlings and incubate for about 7 days in growth. Obtain mushroom mycelium and its culture solution.
본 발명에 따른 버섯균사체 배양방법은 다음과 같다.Mushroom mycelium culture method according to the present invention is as follows.
균주는 버섯균사체이다.The strain is a mushroom mycelium.
균주 계대배양용 배지는, 감자 포도당 한천배지(potato dextrose agr medium;PDA) 20g을 증류수 1리터에 녹인 후 121℃에서 15분간 고압습윤 멸균하여 일회용 페트리 디쉬(dish)에 20ml씩 부어 평판 배지를 만들어 사용한다.For strain passage culture medium, 20 g of potato dextrose agr medium (PDA) was dissolved in 1 liter of distilled water and sterilized by autoclaving at 121 ° C. for 15 minutes at 20 ° C. in a disposable petri dish to make a flat medium. use.
전(前)배양용 액체배지는, 증류수 1리터에 중량비율로 포도당 6g, 맥아당 1.8g, 효모추출물 1.2g, 맥아추출물 6g을 녹여 pH 6.0으로 하여 121℃에서 15분간 고압습윤 멸균하여 전 배양용 액체 배지로 사용한다.The preculture liquid medium is dissolved in 6 liters of glucose, malt sugar 1.8g, yeast extract 1.2g, malt extract 6g in 1 liter of distilled water to pH 6.0 and sterilized by autoclaving at 121 ° C for 15 minutes for pre-culture. Use as a liquid medium.
본(本) 배양용 액제배지는, 감귤가공공장에서 감귤농축액을 중량비율로 물 1리터에 5~30%가 되게 녹여 121℃에서 15~20분간 고압습윤 멸균하여 본 배양용 배지로 사용한다.This culture medium is used as a culture medium by dissolving the citrus concentrate in a citrus processing plant to 5-30% in 1 liter of water at a weight ratio and sterilizing by autoclaving at 121 ° C. for 15-20 minutes.
이와 같은 균주, 균주 계대 배양용 배지, 전배양용 배지 및 본배양용 액체 배지를 사용하는 버섯균사체의 배양은 다음의 실시예와 같이 하였다.Cultivation of the mushroom mycelium using such a strain, strain passage culture medium, preculture medium, and the liquid culture medium for this culture was carried out as in the following examples.
(실시예1)Example 1
계대 배양된 버섯균사체를 가로세로 3mm 되게 잘라 500ml 삼각플라스크에 전배양용 액체 배지 100ml에서 10일간 전배양한 후 1000ml 삼각플라스크에 전배양용 액체배지 300ml에 접종한다. 이를 5일간 배양한 후 본 배양용 종균으로 사용하였다.The cultured mushroom mycelium was subcutaneously cut to 3 mm in length, and then incubated in 500 ml Erlenmeyer flasks for 100 days in 100 ml of preculture liquid medium, and then inoculated in 1000 ml Erlenmeyer flasks for 300 ml of preculture. This was incubated for 5 days and used as a seed for the culture.
최대 배양용량이 약 7리터인 소형발효조에 감귤농축액 626g을 물 5리터로 희석하여 본 배양액을 만들어 121℃에서 15분간 고압습윤 멸균한 후 본 배양용 종균을 접종하였다.In a small fermentation tank with a maximum culture capacity of about 7 liters, 626 g of citrus concentrates were diluted with 5 liters of water to make the culture solution, and the culture medium was inoculated with high-pressure wet sterilization at 121 ° C. for 15 minutes.
총 배양 기간을 6일로 하였고 그 결과는 도 1의 발효(fermenter) 기록표와 같이 나타났다.The total incubation period was 6 days and the results are shown in the fermenter record table of FIG.
도 1의 발효기록표에 따르면, 일정한 농도(pH)조건에서 건체량이 증가되어 안정적으로 발효되고 있음을 알 수 있다.According to the fermentation record table of Figure 1, it can be seen that the dry weight is increased and stable fermentation under a constant concentration (pH) conditions.
따라서, 버섯균사체 배양을 위해서는 각각의 성장 특성에 따라 배지의 조성을 달리해야 하지만 본 발명의 경우 상황버섯 균사체, 낙옆송층버섯 균사체, 영지버섯 균사체, 표고버섯 균사체, 운지버섯 균사체 등의 배양에 있어서 특별한 첨가물이 없이도 대량생산이 가능하고, 대량 배양된 버섯균사체를 버섯자실체 형성을 위한 종균으로 이용할 수 있다.Therefore, in order to cultivate mushroom mycelium, the composition of the medium should be different according to the growth characteristics, but in the present invention, a special additive in cultivation of the situation mushroom mycelium, Lactobacillus fungus mycelium, Ganoderma lucidum mycelium, shiitake mycelium, and fingerling mushroom mycelium Mass production is possible without this, and mass cultured mushroom mycelium can be used as a seed for forming mushroom fruiting bodies.
(실시예2)Example 2
실시예 1에서 배양된 버섯균사체와 배양액을 이용하여 혈액종양세포(HL-60)에 대한 항암 효과가 있는지를 알기 위해 검색하였다.The mushroom mycelium and the culture medium cultured in Example 1 were used to find out whether there was an anticancer effect on blood tumor cells (HL-60).
이 검색에서 이용되는 시료의 추출은, 배양된 버섯균사체를 85℃의 뜨거운 물로 24시간 추출한 후 에탄올로 침전시킨 후 동결건조하여 이를 시료 A로 하고, 배양액을 그대로 20배 진공 농축하여 시료 B로 하였다.Extraction of the sample used in this search was carried out by extracting the cultured mushroom mycelium with hot water at 85 ° C. for 24 hours, precipitating with ethanol, lyophilizing it to make Sample A, and concentrating the culture solution in vacuum 20 times to make Sample B. .
이렇게 준비된 시료 A와 B를 인산완충살린(PBS)과 에탄올을 같은 비율로 혼합한 용애에 녹인 후 막분리 여과지를 이용하여 분자량 3,000이하의 물질과 분자량 3,000이상의 물질로 분리하여 A1,A2,B1,B2로 하여 검정시료로 하였다.Thus prepared samples A and B were dissolved in a solvent mixed with phosphate buffered saline (PBS) and ethanol in the same ratio, and then separated into a substance having a molecular weight of 3,000 or less and a molecular weight of 3,000 or more using a membrane filter paper to obtain A1, A2, B1, B2 was used as the assay sample.
그리고, 세포배양은, 급성전골수성 백혈병 환자에서 유래한 HL-60 세포주를 한국 세포주 은행(KCLB)으로부터 분양 받아 100units/ml의 페니실린-스트렙토마이신과 10%의 FBS(fetal bovine serum)가 함유된 RPMⅡ 1640 배지를 사용하여 37℃, 5%이산화탄소 항온기에서 배양하였으며, 계대 배양은 3~4일에 한번씩 실시하였다.In addition, the cell culture, RPMII containing 100units / ml of penicillin-streptomycin and 10% of FBS (fetal bovine serum) by receiving HL-60 cell line derived from acute promyelocytic leukemia patients from Korea Cell Line Bank (KCLB) 1640 medium was used to incubate at 37 ℃, 5% carbon dioxide incubator, subculture was performed once every 3 to 4 days.
세포의 대사활성측정은, HL-60 세포를 3×105cell/ml의 농도로 96well plate(웰 플레이트)의 각 well에 넣고 준비한 검정시료 A1,A2,B1,B2를 100ug/ml의농도로 첨가하였다. 이를 4일간 배양한 다음, 3-(4,5-dimehtylthiazol)-2,5-diphenylterazolium bromid(MTT) 100ug을 첨가하고 4시간 동안 더 배양하였다. 플레이트(plate)를 1,000rpm에서 10분간 원심분리하고 조심스럽게 배지를 제거한 다음 dimethylsulfoxide(DMSO) 150ug를 가하여 540nm에서 흡광도를 측정하였다. 각 시료군에 대한 평균흡광도 값을 구하였으며, 대조군의 흡광도 값과 비교하여 성장억제속도를 조사하였고 그 결과는 도 2의 표와 같았다.For measuring the metabolic activity of the cells, assay samples A1, A2, B1, and B2 were added to each well of a 96 well plate (well plate) at a concentration of 3 x 105 cells / ml, and the concentration of 100 ug / ml was added. . After incubating for 4 days, 100 ug of 3- (4,5-dimehtylthiazol) -2,5-diphenylterazolium bromid (MTT) was added and further incubated for 4 hours. The plate was centrifuged at 1,000 rpm for 10 minutes, the medium was carefully removed, and 150 ug of dimethylsulfoxide (DMSO) was added to measure absorbance at 540 nm. The average absorbance value for each sample group was obtained, and the growth inhibition rate was investigated by comparing with the absorbance value of the control group, and the results are shown in the table of FIG. 2.
도 2의 표에 따르면, 분자량 3,000 이상의 균사체 추출물인 검정시료 A1의 억제율은 30.581이고, 분자량 3,000이하의 균사체 추출물인 검정시료 A2의 억제율은 47.840으로서, 검정시료(A1/A2)의 분자량에 따라 억제율은 다르게 나타나고 있음을 알 수 있다.According to the table of FIG. 2, the inhibition rate of assay sample A1, which is a mycelium extract having a molecular weight of 3,000 or more, was 30.581, and the inhibition rate of assay sample A2, which is a mycelium extract having a molecular weight of 3,000 or less, was 47.840, and the inhibition rate was determined according to the molecular weight of the assay sample (A1 / A2). It can be seen that is shown differently.
또한, 분자량 3,000이상의 배양액 추출물인 검정시료 B1의 억제율은 39.901이고, 분자량 3,000이하의 배양액 추출물인 검정시료 B2의 억제율은 39.753으로서, 배양액 추출물의 억제율은 분자량에 크게 영향 받지 않음을 알 수 있으며, 공통적으로는 균사체 추출물-배양액 추출물 들은 분자량에 어느정도 영향을 받고 있으나 HL-60세포에 대한 일정한 억제율을 나타내어 항암 작용이 있음을 알 수 있고, 이는 간 기능 개선과 면역계의 활성을 증가 시키는 단백 다당체로 이용되거나, 같은 약리 작용의 기능성 식품 소재 및 기능성 물질, 양식어류 및 동물사료 등의 첨가물로서의 이용 가능성이 있음을 나타낸다.In addition, the inhibition rate of assay sample B1, which is a culture extract of molecular weight 3,000 or more, is 39.901, and the inhibition rate of assay sample B2, which is a culture extract of molecular weight 3,000 or less, is 39.753, indicating that the inhibition rate of culture extract is not significantly affected by molecular weight. As mycelium extract-culture extracts have some effect on the molecular weight, they show a certain inhibitory rate on HL-60 cells, indicating that they have anti-cancer activity, which are used as protein polysaccharides to improve liver function and increase the activity of the immune system. In addition, it indicates the possibility of use as additives, such as functional food materials and functional substances, farmed fish and animal feed of the same pharmacological action.
여기서, A1.A2.B1.B2 검정시료의 HL-60 세포에 대한 억제율은,(control(컨트롤)흡광도-시료흡광도)/control(컨트롤)흡광도×100으로 구한 값이다.Here, the inhibition rate for the HL-60 cells of the A1.A2.B1.B2 assay sample is a value obtained by (control absorbance-sample absorbance) / control absorbance × 100.
이와 같이 본 발명은 감귤농축액을 이용하여 버섯균사체를 배양함으로서, 상황버섯 균사체, 낙옆송층버섯 균사체, 영지버섯 균사체, 표고버섯 균사체, 운지버섯 균사체 등의 배양에 있어서 특별한 첨가물이 없이도 대량생산이 가능하고, 대량 배양된 버섯균사체를 버섯자실체 형성을 위한 종균으로 이용할 수 있는 효과가 있다.As described above, the present invention cultivates mushroom mycelium by using citrus concentrates, and it is possible to mass-produce without special additives in the cultivation of the situation mushroom mycelium, Lactobacillus fungus mycelium, Ganoderma lucidum mycelium, shiitake mushroom mycelium, and fingering mushroom mycelium. , Mass cultured mushroom mycelium has the effect that can be used as a spawn for mushroom fruit body formation.
또한 본 발명의 배양방법은 비교적 짧은 기간에 버섯균사체를 대량으로 배양할 수 있으며, 그 배양으로부터 기능성 물질을 추출할 수 있는 효과가 있으며, 버섯균사체 배양에 있어서 안정성이 보장된 배지를 이용함으로서, 그 배양액을 기능성 식품 원료, 양식어류 및 동물사료 첨가재 등으로 사용할 수 있게 하는 효과가 있다.In addition, the culture method of the present invention can cultivate a large number of mushroom mycelium in a relatively short period of time, there is an effect that can be extracted from the functional material from the culture, by using a medium which is guaranteed stability in the culture of mushroom mycelium, There is an effect that can be used as a functional food raw material, aquaculture fish and animal feed additives.
또한, 수득되는 버섯균사체 및 배양액은 HL-60세포에 대한 일정한 억제율을 나타내어 항암 및 면역계의 활성을 증가시키는 단백 다당체로 제조되어 이용 범위가 큰 효과가 있다.In addition, the obtained mushroom mycelium and the culture medium is produced with a protein polysaccharide to increase the activity of the anti-cancer and immune system by showing a constant inhibition rate for HL-60 cells has a large range of applications.
그리고, 버섯균사체 배양을 위한 배지로서 감귤농축액을 이용함으로서 비상품성 감귤의 이용성을 다양화시켜, 감귤의 상품성을 제고 시킬 수 있는 효과도 있으며, 버섯균사체의 배양에 따른 대사산물의 추출과 균사체의 회수에 있어서 저비용 고효율화가 가능한 효과가 있다.In addition, by using a citrus concentrate as a medium for cultivating mushroom mycelium, it is possible to diversify the usability of non-commercial citrus fruit and enhance the merchandise of citrus fruit. The extraction of metabolite and recovery of mycelium by cultivating mushroom mycelium Low cost and high efficiency can be achieved.
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Cited By (4)
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| KR100432472B1 (en) * | 2002-03-22 | 2004-05-20 | 이광현 | A method for producing furit bodies and mycelia of cordyceps spp. in large scale |
| KR100756526B1 (en) | 2006-09-05 | 2007-09-10 | 박승림 | Manufacturing method of tangerine culture ground for growing mushroom using microwave and food of processed mushroom |
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| KR20150104301A (en) * | 2014-03-05 | 2015-09-15 | 김영민 | Fruit beverage cultured dietary fiber using mushroom mycelium and manufacturing method thereof |
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| KR100772941B1 (en) * | 2006-11-30 | 2007-11-05 | 오병권 | Plant medium using bamboo extracts and citrus juice, and mass propagation method of adventitious root contained saponin using that |
| KR102242932B1 (en) * | 2020-10-16 | 2021-04-20 | 곽병조 | Mask for antibacterial and antimicrobial |
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