JPS63131067A - Pumping distributor - Google Patents

Abstract

PURPOSE:To enable treatment of a large amount of material quickly, by arranging a cylinder to move a needle member of a suction/drain device to a desired position, above and below a cleaning liquid, a solution and the like and to the right and left thereof and an operating section to achieve the pumping and distribution by operating the suction/drain device. CONSTITUTION:A suction/drain device 3 is linked to an operating section 14 using a syringe comprising a needle member 8 and operated with the vertical movement of the operating section 14. The suction/drain device 3 is moved longitudinally or vertically relative to a cleaning liquid 13 and a container 9 filter body 12. Then, a primary pumping distribution mechanism 2 moves a first suction/drain device 3 to a desired position longitudinally with a first horizontal cylinder 4. With the reduction of the cylinder 4, the suction/drain device 3 is moved onto the cleaning liquid 13; with the extension thereof, it is moved onto a solution 11. A secondary pumping distribution mechanism 2' moves a second suction/drain device 3' to a desired position longitudinally with second and third horizontal cylinders 4' and 4''. With the reduction of both the cylinders, the suction/drain device 3' is moved onto the cleaning liquid 13; with the extension of the cylinder 4', it is moved onto the solution 11. With the extension of both the cylinders, it is moved onto the filter body 12.

Description

Detailed Description of the Invention [Industrial Application Field] The present invention is directed to dispersing materials such as aggregated cells in a solution by pumping, and dispensing a solution containing the material onto the filtering surface of a filter body. The present invention relates to a pumping dispensing mechanism and a pumping dispensing device configured by arranging a plurality of pumping dispensing mechanisms in parallel.

[Conventional technology]

To prepare materials such as cells in a solution, 1. Normally, first, the suction device is pumped in a cleaning solution to wash it, first, the material in the solution is pumped and dispersed by the suction device, and then the material is pumped inside the suction device. Inhale.

Dispense onto the filtering surface of the filter body.

Conventionally, these steps have often been performed one by one manually or with simple equipment, and no means have been taken to achieve them completely automatically.

[Problem that the invention seeks to solve]

When pumping is done manually as in the past, it is difficult to always apply a constant force by hand, so you may end up applying more force than necessary.

This often resulted in the destruction of cells and other materials.

Furthermore, manual processing has a limit to its throughput, making it impossible to process large amounts of materials quickly.

The present invention is intended to solve the above-mentioned problems and allows for rapid pumping of large amounts of material without destroying it.

The purpose is to provide a device that can dispense.

[Means to solve the problem]

Therefore, the pumping dispensing mechanism 2 according to the present invention has a needle body 8 equipped with a diameter suitable for the purpose of isolating cell debris at the lower end.
The suction/exhaust device 3 having a vacuum cleaner 3 is moved back and forth relative to the cleaning liquid 13 and the solution 11.

The filter body 12 is connected to a horizontal cylinder 4 which is relatively moved back and forth to a desired position on the filtration surface.

The suction/exhaust device 3 is moved up and down relatively, and when the suction/exhaust device 3 reaches its lower limit, the needle body 8 of the suction/exhaust device 3 is moved into the cleaning liquid 13, into the solution 11, or directly above the filtration surface of the filter body 12, etc. A vertical cylinder 5 is provided for moving into position.

In order to achieve pumping and dispensing of the suction/discharge device 3, the pumping/dispensing mechanism 2 is configured by providing an operating section 14 for operating the suction/discharge device.

Furthermore, by arranging a plurality of pumping dispensing mechanisms 2 in parallel, a pumping dispensing device 1 capable of performing pumping in multiple stages according to the diameter of the needle body 8 was constructed. in this case.

The diameter of the suction/discharge device needle 80 was set to be larger for the pumping dispensing mechanism 2 in the rear row and smaller toward the pumping dispensing mechanism 2 in the front row.

Note that the filter body 12 was conveyed to the next step either manually or automatically.

[Effect]

The pumping dispensing mechanism 2 according to the present invention configured as described above has the following features:
First, the needle body 8 is relatively moved onto the cleaning liquid 13 by the horizontal cylinder 4 and relatively lowered by the vertical cylinder 5, so that the needle body 8 enters the cleaning liquid 13 to a position. In this state, the second operating section 14 operates the suction/exhaust device 3 to clean it. Pump &13 to clean the inside of the suction/exhaust device 3.

When the cleaning is completed, the suction/exhaust device 3 relatively rises, moves horizontally, and further descends.
The needle body 8 is inserted into the solution 11.

Here, material 10 such as cell pieces solidified in solution 11
Pump and isolate. When this pumping is achieved, inhale the solution 11 containing the material 10, rise, level,
It moves downward and dispenses onto the filtering surface of the filter body 12.

When this dispensing is completed, the needle body 8 moves upward, horizontally, and downward again to bring the needle body 8 into the cleaning liquid 13 and performs pumping to clean the inside of the suction/discharge device 3.

Repeat the above operations.

Moreover, in the pumping dispensing device 1 configured by installing a plurality of pumping dispensing mechanisms 2 in parallel in the front and back.

Pumping of the solution 11 starts from the rear pumping and dispensing mechanism 2 and is accomplished by the previous pumping and dispensing mechanism 2 in turn. The diameter of the suction and evacuation device needle 8 is set to become smaller toward the pumping dispensing mechanism 2 in the front row, so that the material 1
0 will be gradually isolated in finer pieces.

When the pumping by all the pumping and dispensing mechanisms 2 is completed, the solution 11 is dispensed onto the filter surface by the pumping and dispensing mechanism 2 in the front row. Each pumping dispensing mechanism 2 has a vertical cylinder 5 and an operating part 14, and can move and operate independently.
For example, while the pumping dispensing mechanism 2 in the front row is dispensing, the pumping dispensing mechanism 2 in the rear row can perform different tasks, such as cleaning.

〔Example〕

An embodiment of the present invention will be described with reference to FIG.

Figures 1 and 2 show two pumping dispensing mechanisms 2.2''
This shows a pumping dispensing device 1 in which
FIG. 3 shows a preparation preparation device 19 in which the pumping dispensing device 1 is one of the constituent elements.

In the pumping dispensing device 1 which is composed of these two pumping dispensing mechanisms 2.2, the pumping dispensing mechanism in the rear row is the primary pumping dispensing mechanism 2 that pumps the solution 11 for the first time, and the pumping dispensing mechanism in the front row is the primary pumping dispensing mechanism 2 that pumps the solution 11 for the first time. This is a secondary pumping and dispensing mechanism 2' that performs pumping. In this case, naturally, the diameter of the suction/discharge device needle 8 of the secondary pumping dispensing mechanism 2' is set larger than that of the secondary pumping dispensing mechanism 2'.

The suction/exhaust device 3 here has a cylindrical body 6. Using a syringe consisting of a needle 8° and a pressure piece 7.

The mechanism is such that the suction and evacuation device 3 is operated by connecting the actuating portion 14 and the pressure piece 7 and moving the two actuating portions 14 up and down. It is fine as long as it has a suction and exhaust function.

Further, this suction/exhaust device 3 is configured to discharge a cleaning liquid 13. Container 9. It moves back and forth or moves up and down relative to the filter body 12. In this embodiment, the suction/discharge device 3 is moved, but instead of moving the suction/discharge device 3, the cleaning liquid 13.
Container 9. The filter body 17 may be moved by the horizontal cylinder 4 and the vertical cylinder 5.

The primary banging dispensing mechanism 2 moves the first suction/exhaust device 3 to the desired position forward or backward by means of a first horizontal cylinder 4. When the first horizontal cylinder 4 is contracted, the first suction/exhaust device 3 is It is moved to a position above the cleaning liquid 13, and above the solution 11 in the extended state.

The secondary pumping dispensing mechanism 2 is a second horizontal cylinder 4
the second suction pump 3 by ``and the third horizontal cylinder 4''
′ is moved back and forth to the target position.

When both are contracted, the second suction and evacuation device 3° is above the cleaning liquid 13, and when the second horizontal cylinder 4'' is extended, the solution 11 is
When the top and both are extended, the filter body 12 is positioned on the filtration surface.

In this embodiment, after the second pumping is completed, the container 9 containing the solution 11 is pushed forward by the extrusion section 15 and positioned directly below the second suction/exhaust device 3 degrees. In this case, the second suction/exhaust device 3' may be moved to be positioned above the solution 11.

No.-Suction/exhaust device 3. The number of the second suction/exhaust device 3' is not limited and may be set according to the requirements.

Further, in this embodiment, the first suction/exhaust device 3 and the second suction/exhaust device 3° are supported by two identical guide rails 18 and move in the front-rear direction, but separate guide rails are provided for each. It's okay.

The filter body 12 is held by a filter body 12 holder, and is automatically conveyed by an extrusion cylinder 16 to a chemical processing device, which is the next step. This conveying means is not limited to this extrusion cylinder 16, but may be conveyed by a belt conveyor or the like.

In addition, in FIG. 3, the filter body 12 into which the material lO has been dispensed onto the filtration surface by the pumping dispensing device 1 according to the present invention is held in the filter body holder 17 and is subjected to the first step of chemical treatment. It is conveyed to a processing device 20, and then further conveyed to a transfer device 21 to create a slide.

〔Effect of the invention〕

In this way, according to the present invention, pumping is achieved mechanically, so it can always be performed with a constant force, and therefore materials such as cells can be destroyed by applying more force than necessary as in the past. Things are gone.

Moreover, since it is carried out automatically by one machine, it exhibits excellent effects such as being able to quickly process a large amount of material.

[Brief explanation of the drawing]

FIG. 1 is a front view showing a pumping dispensing device 1 configured by installing two pumping dispensing mechanisms according to the present invention in parallel in front and back, FIG. 2 is a side view thereof, and FIG. 3 is a pumping dispensing device 1 according to the present invention. This figure shows a slide preparation device in which the device 1 is one of the constituent elements. Explanation of symbols 1: Pumping dispensing device. 2: Pumping dispensing mechanism, 3: First suction and evacuation device. 3": Second suction/exhaust device, 4: First horizontal cylinder - 94"
:Second horizontal cylinder. 4": Third horizontal cylinder. 5: First vertical cylinder -9 5°: Second vertical cylinder, 6: Cylindrical body. 7: Pressure piece, 8: Needle body, 9: Container. 10: Material, 11: Solution , 12: Filter body. 13: Cleaning liquid, 14: Operating part, 15: Extrusion part. 16: Extrusion cylinder, 17: Filter integrally held odor. 18 Ni guide rail, 19 Nibre barad making device. 20: Chemical processing equipment, 21: Transfer device.

Claims (5)

[Claims] (1) A needle body (8) with a diameter suitable for the purpose of isolating a material (10) such as a cell piece is provided at the lower end, and the material is housed in a container (9) such as a test tube. While pumping the solution (11) containing (10), the solution (11) is
A filter body that is manually or automatically conveyed to the next process (
The suction device (3) used to dispense onto the filtration surface of (12)
), the suction device (3), the cleaning liquid (13), and the container (11).
, and the filter body (12) are moved back and forth relative to each other, and the suction/discharge device (3) is placed on the cleaning liquid (13), on the solution (11), on the filtration surface of the filter body (12), etc. a horizontal cylinder (4) to be moved to a position, the suction/exhaust device (3), the cleaning liquid (13), and the container (
11), move the filter body (12) up and down relative to each other, and with the suction/evacuation device (3) reaching its lower limit, move the needle (8) of the suction/evacuation device (3) to the cleaning liquid (13). Medium, solution (1
1) Inside, a vertical cylinder (5) that moves the filter body (12) to a vertical target position, such as directly above the filtration surface, and an actuator (14) that operates the suction device (3) to accomplish the pumping and dispensing. A pumping dispensing mechanism (2) consisting of. (2) A needle body (8) with a diameter suitable for the purpose of isolating a material (10) such as a cell piece is provided at the lower end, and the material is housed in a container (9) such as a test tube. While pumping the solution (11) containing (10), the solution (11) is
A filter body that is manually or automatically conveyed to the next process (
The suction device (3) used to dispense onto the filtration surface of (12)
), the suction device (3), the cleaning liquid (13), and the container (11).
, and the filter body (12) are moved back and forth relative to each other, and the suction/discharge device (3) is placed on the cleaning liquid (13), on the solution (11), on the filtration surface of the filter body (12), etc. a horizontal cylinder (4) to be moved to a position, the suction/exhaust device (3), the cleaning liquid (13), and the container (
11), move the filter body (12) up and down relative to each other, and with the suction/evacuation device (3) reaching its lower limit, move the needle (8) of the suction/evacuation device (3) to the cleaning liquid (13). Medium, solution (1
1) Inside, a vertical cylinder (5) that moves the filter body (12) to a vertical target position, such as directly above the filtration surface, and an actuator (14) that operates the suction device (3) to accomplish the pumping and dispensing. A pumping dispensing mechanism (2) consisting of
A pumping dispensing device (1) consisting of a plurality of pumping dispensing devices installed in parallel. (3) The pumping dispensing device (1) according to claim 2, wherein the diameter of the suction and evacuation device needle (8) is set to be smaller as the pumping mechanism is located in the front row. (4) Pour the filter body (12), solution (11) and cleaning liquid (13) from the front.
), a cleaning liquid (13), and a pumping dispensing mechanism according to claim 1, and a pumping dispensing device (1) according to claim 2. (5) As set forth in claim 1, the extrusion cylinder (16) for pushing out the filter body (12) forward is provided as a means for automatically conveying the filter body (12) to the next step. a pumping dispensing mechanism (2), and a pumping dispensing device according to claim 2 (
1).