JPS6128941B2 - - Google Patents

Description

Detailed Description of the Invention This invention allows reagents such as antiserum used in clinical tests using agglutination reactions to be divided and stored in the amount necessary for one test, and used as is as a reaction container during the test. This invention relates to a reaction vessel capable of

As is well known, the agglutination reaction, which is a type of antigen-antibody reaction, is applied to various tests in the field of clinical testing.

By the way, in the agglutination reaction test method that is generally adopted when performing such various tests,
In both cases, there are separate containers for storing reagents such as antibody serum and reaction containers such as glass plates and microplates that cause reactions between the reagents and sample serum. It is necessary to dispense reagents into reaction vessels each time, and this operation is performed manually, which is extremely complicated, and the problem is that the complexity increases significantly when testing and processing a large amount of specimens. It was hot.

When testing and processing such a large amount of specimens, it is necessary to perform a series of operations such as mixing and shaking quickly after performing the dispensing process as described above, but in conventional reaction vessels, reagents are kept open. Because each operation is performed under such conditions, the reagents evaporate during these operations, which is one of the causes of false judgments.Moreover, the reagents may contain viruses, etc. that are harmful to the human body. Performing the above operation in an open state may lead to extremely unfavorable sanitary results such as people being infected with the virus.

Further, the various reaction vessels conventionally used are mainly used for visual judgment, and no consideration has been given to using optical means to detect the presence or absence of aggregates.

Furthermore, in conventional test methods, in addition to the complexity of dispensing reagents into reaction vessels, agglutination reactions are suppressed under conditions of excess antigen or excess antibody.
In order to avoid the so-called zone phenomenon, if it is necessary to dilute the sample serum etc. at several dilution ratios,
In addition to adding work such as diluting and dispensing onto the reaction vessel using a diluter, etc., for example, ABO
As in the case of a formula blood type determination test, if sufficient results cannot be obtained unless the reactions of one sample, such as serum or blood, and different types of reagents are tested at the same time, the same sample may be reacted with different types of reagents. In addition to dispensing the sample into multiple containers, it is also necessary to dispense different types of reagents for each sample, which requires an extremely large amount of labor, and can also lead to erroneous judgments due to dispensing errors. It happened frequently.

The present invention has been made to solve these problems, and it enables reagent storage and agglutination reaction to be performed in the same container, allows it to be used as it is as an optical measurement cell, and allows reagent evaporation. The purpose of this is to prevent personnel such as laboratory personnel and testers from coming into direct contact with the reaction solution.

Embodiments of the present invention will be described in detail below with reference to the drawings.

FIG. 1 shows a basic embodiment of the present invention, in which a reaction vessel 1 is formed of a rectangular flat plate 2 made of plastic, glass, etc., and has circular recesses 33, 34 arranged in two vertical rows and three horizontal rows. It is formed by forming. Among the recesses 33, 34..., the recesses 33 in one row (on the left in the figure) are used for reagent storage, and the recesses 34 in the other row are used for reaction. Reagents, buffer solutions, and the like used for measuring agglutination reactions are dispensed in predetermined amounts into the reagent storage recesses 33 by an automatic dispenser or the like. The corresponding reagent storage recess 33 and reaction recess 34 are communicated with each other by a groove 5, and the reagent can be transferred from the storage recess 33 to the reaction recess 34 during the agglutination reaction. It's becoming like that. An air-impermeable transparent film or sheet 4 (hereinafter simply referred to as film 4) is attached to the upper surface of the plate 2 to protect and seal the reagents filled in each of the recesses 3.

The recesses 33 and 34 have a required depth, and for example, as shown in FIG. 2A, the bottom surface 3a is formed into a flat dish shape, or as shown in FIG. A curved bulge on the side,
Alternatively, as shown in Figure C, the bottom surface 3a may be curved and bulged, and the center thereof may be further protruded downward.

Further, the above-mentioned film is made of PVC, polyvinylidene chloride, etc., which is non-porous, does not elute plasticizers, and can be handled under hygienic conditions, and also includes a portion of metal film such as aluminum film. These are adhered to the upper surface of the plate 2 using heat, adhesive, or the like.

FIG. 3 shows an embodiment in which the film 4 includes a metal film 10, and the metal film 10 includes:
A hole 4a is formed in the film 4 at the center of the recesses 33 and 34, and the film is attached to close the hole 4a. In this way, after pasting the film 4 on the plate 2, a fixed amount of reagent is injected from the hole 4a, and then the metal foil 10 is pasted to achieve sealing. Injection is also easy. Of course, since the other parts are transparent, it is possible to observe the reaction taking place within the recess.

FIG. 4 shows another embodiment of the above film. This film 20 consists of double layers 21 and 22, and the upper layer 21 is made of polyvinyl chloride, polyvinylidene chloride, etc. as in the above embodiment. It consists of a thick film that is not gas permeable, and the lower layer 22 has some gas permeability, and although its properties for protecting the internal reagents are inferior, it prevents the internal reagents from evaporating and scattering temporarily or during testing. The membrane has sufficient properties for this purpose, and has a membrane strength that allows it to be easily penetrated with the tip of a pipette or serum dispensing nozzle. It is a thin film made of

These upper and lower layers 21 and 22 are airtightly adhered via a removable adhesive or the like, and the upper layer 21 can be peeled off from the lower layer 22 during the cohesion reaction test.

Next, when actually using the reaction container 1 configured as described above, when the container 1 is tilted at a certain angle from the horizontal, the reagent in the storage recess 33 moves through the groove 5 to the reaction recess 34. Can be transferred. After that, pipette 30 (or dispensing nozzle when dispensing automatically with an automatic dispensing machine) as shown in Figure 5 A.
is inserted into the film 4 and dispensed into each recess 34, respectively. Thereafter, the container 1 is shaken to promote the reaction with the reagent transferred to the recess 34, and then left to stand still to confirm the presence of aggregates due to the reaction between the two.

In addition, in the embodiment, the film 4 has a configuration in which the pin hole created by the pin hole is closed again by self-recovery after being penetrated as shown in FIG. In either case, evaporation of the reaction solution and infection of the human body are prevented.

Further, in the above embodiment, if at least the recess 34 of the reaction vessel 1 is made of a transparent material such as transparent glass or transparent plastic, the reaction vessel can be used as it is as a cell for optical measurement. , it becomes possible to recognize the measurement results quantitatively.

Furthermore, when performing an ABO blood type determination test, in which a sufficient result cannot be obtained unless the reaction between one sample of serum or blood and different types of reagents is tested at the same time, each of the above wells may be used. If a fixed amount of reagents and buffer solutions corresponding to each reaction are filled in advance in the chamber 33, and a label indicating the type of reaction is printed or pasted on the plate surface near each corresponding well 34, each well By simply injecting each sample into the test tube 34, a plurality of test results based on different reactions can be obtained simultaneously.

Furthermore, if it is necessary to avoid a zone phenomenon due to excess antigen or antibody, each recess 33 is filled in advance with several types of the same reagent at a concentration approximately equivalent to the reaction equivalent, and the concentration is indicated on the plate surface. By doing so, even when the same concentration and the same amount of sample serum, etc. are dispensed into each well 34, the concentration ratio of antigen and antibody in any one of the wells 34 can be maintained. can be optimized, avoid zone phenomena, and avoid inspection errors.

This example has the advantage that the tip of the pipette etc. is not contaminated by the reagent when dispensing the sample, and since the reaction is carried out simultaneously after the dispensing operation, it is particularly convenient for observing the behavior of the reaction over time, etc. .

Note that other aspects of this embodiment are similar to the various changes in the first embodiment described above, so their explanation will be omitted.

As described above, the present invention allows the storage of reagents and the dispensing and reaction of specimens to be carried out in the same container; can be carried out in the same container in a completely sealed state. Therefore, compared to conventionally proposed containers, in which the film is turned over and the reagent is returned to liquid form in an open state, and the sample is injected into the container and reacted, it is easier to handle, and the reagent is If impurities are mixed in during evaporation or reaction, or if an inspector or the like carelessly touches the reaction solution directly, stains will not occur.

Furthermore, since the reaction cavity is made light permeable, the container can be used as it is as a cell for optical measurement applied to optical means.

[Brief explanation of the drawing]

FIG. 1 is a perspective view of a reaction vessel showing a basic embodiment of the present invention, FIGS.
The figures are partial cross-sectional views showing various forms of the film, and FIGS. DESCRIPTION OF SYMBOLS 1... Reaction container, 2... Plate, 33... Recess for reagent storage, 34... Recess for reaction, 4, 20... Film, 5... Groove, 4a... Hole, 10... Metal foil, 21... Upper layer, 22... Lower layer .

Claims (1)

[Scope of Claims] 1. A reaction vessel used when measuring agglutination reactions, etc., which is formed by dividing a plurality of recesses into a plate on a flat plate into one for reagent storage and one for reaction. are connected to each other by grooves so that the reagent can be transferred to the reaction recess during the reaction, and at least one of the recesses is formed to be light-transmissible; A reagent is dispensed in advance into the storage cavity, a transparent film is pasted and sealed on the top surface of the plate, and a sample dispensing nozzle or the like can be inserted through the corresponding part of the reaction cavity. A reaction vessel characterized in that it has a membrane structure that is sealed after the reaction. 2. The reaction vessel according to claim 1, wherein a hole is formed in the film at a position corresponding to the reaction recess, and the hole is closed by pasting a metal foil. 3 The film is formed of two layers, an upper layer and a lower layer, and the upper layer is made removable using a releasable adhesive, and the lower layer is provided with a self-returning force so that it closes again after the dispensing nozzle, etc. passes through it. The reaction vessel according to claim 1, characterized in that the reaction vessel is formed of a thin film comprising: