JPS61151129A - Absorbefacient for vitamin e - Google Patents

Abstract

PURPOSE:To provide an agent to promote the absorption of vitamin E administered to man, domestic animal and domestic fowl, by using Clostridium butyricum Miyairi as an active component. CONSTITUTION:Clostridium butyricum Miyairi is used as an active compound of the titled agent. Clostridium butyricum Miyairi is an anti-putrefactive butyric bacillus known as Miyairi bacillus having the shape of straight of slightly curved rod with blunt ends at both sides. It can be administered together with vitamin E to promote the absorption and improve the pharmacological effect of the vitamin. The intestinal absorption of vitamin E can be promoted with the present agent even in the case of the administration of vitamin E orally as a solid drug. The Miyairi bacillus is added preferably in an amount of 10-2,000mg to 1kg of feed.

Description

DETAILED DESCRIPTION OF THE INVENTION Object of the Invention (Field of Industrial Application) The present invention relates to a vitamin E absorption enhancer administered to humans, livestock and poultry.

(Conventional technology) Vitamin E has pharmacological effects such as preventing the generation of lipid peroxide derived from polyunsaturated fatty acids in the body, strengthening blood vessels, improving disease resistance, and activating cells, and is an important substance for the human body. It is one of the vitamins.

Currently, many health foods containing vitamin E as a main ingredient are on the market, focusing on these pharmacological effects, and as there have been no reports of drug-induced harm so far, large doses are required to increase their pharmacological effects. It is often done.

On the other hand, for livestock and poultry whose staple diet is artificial feed, administration of vitamin E is essential to prevent deficiency diseases, and large doses are also used for the purpose of improving disease resistance and shortening the recurrence of estrus. ing. However, when vitamin E is mixed into feed in powder form, it is poorly absorbed by livestock and poultry, so it must be administered as a relatively well-absorbed liquid or parenterally as an injection. The current situation is that

(Problems to be solved by the invention) Therefore, in view of the above-mentioned current situation, the present inventors
Efforts were made to achieve effective administration of vitamin E. In particular, efforts were made to achieve effective administration of vitamin E by increasing the rate of intestinal absorption during oral administration, which is the easiest method.

That is, an object of the present invention is to provide a vitamin E absorption enhancer that is effective for humans, livestock, and poultry.

Structure of the Invention (Means for Solving the Problems) The object is to provide a vitamin E product containing Clostridium butyricum Miyairi as an active ingredient.
This is achieved by absorption enhancers.

Clostridium butyricum miyairi (C1ostric), which is the active ingredient of the vitamin E absorption enhancer of the present invention.
liui butyricum Miyairi)
is an anti-septic butyric acid bacterium commonly referred to as Miyajin bacterium (hereinafter abbreviated as Miyajin bacterium). It forms the No side effects are observed when the Miyajin fungus is administered to humans, livestock, and poultry over a long period of time.

Although it depends on the daily age of humans, livestock, and poultry, the amount of Gyujinen is usually administered from 1 to 200+1111 sa/day, preferably from 3 to 20 sa/day per 1 kg of body weight. Moreover, the Miyajin fungus is usually administered in the form of a formulation with a carrier. When used as a carrier for humans, lactose, starch, etc. are used, and the carrier is prepared in the form of powder, granules, tablets, etc., so as to be convenient for oral administration. on the other hand,
When used for livestock and poultry, for example, feed given to each animal is suitable as a carrier, and it is convenient to administer Miyanobacterium when used in combination with the feed. When incorporated into feed, the amount of Miyajinkuni to be added varies depending on the type and age of livestock and poultry, but preferably 10 to 2.0001 g per 1 ko of feed.
0-5oI1g.

In addition, the vitamin E absorption enhancer of the present invention includes vitamin E
In addition to various nutrients such as various vitamins and minerals, and for livestock and poultry, antibacterial feed additives such as antibiotics can also be added.

(Example) Next, the present invention will be described in further detail by giving examples.

Example 1 @Example for Ikuo Daily administration [I (40 g) per Miyanin 1 [cl.

5tridiui butyricum M 1y
airi IF (Feikoken Bibori No. 146)] 2.
A feed additive A having the components shown in Table 1 was prepared by containing 0x108 pieces.

In addition, as a comparative additive, as shown in Table 1, an additive containing the same amount of vitamins as Feed Additive A but no Miyajin bacteria was prepared and designated as Feed Additive B.

Twenty-eight dairy calves were fed with milk replacer and artificial milk having the composition shown in Table 2 from the age of 8 days. During the test period, milk replacer was used ad libitum from 8 days old to 35 days old, and artificial milk was fed ad libitum from 8 days old until the end of the test.

Twenty-eight dairy calves were divided into four groups at the age of 10 days.
In the administration area (10 animals), from 1 to 10 days of age, the above feed additive A was added as feed per day of milk replacer or artificial milk.
In the A control group (10 cows), milk replacer or artificial milk was continued to be fed as feed every day, and in the B agent control group (4 cows), milk substitute or artificial milk was given as feed from 10 days of age. Milk or artificial milk to which 40++ of the feed additive B was added per day was fed to the mice every day, and in the B agent control group (4 animals), milk replacer or artificial milk was fed as feed every day.

Each general-purpose calf, which is a test subject, was 40 years old at the time of introduction (10 days old).
Blood was collected at 1 day, 60 days and 90 days,
The serum vitamin E'a level (μg/d,
9) was surrounded by foot. The results are shown in Table 3 and FIG.

Table 1 Vitamin A 120,0001 U 12
0,0001 U Vitamin D3 2,400 I U
2,400r U vitamin E 1
201 tJ 1201 U Miyanobacterium
2. Ox 108 - Defatted rice bran Remaining amount Remaining amount (margin below) Table 2 Crude protein 28.0% or more 21.0% or more Crude fat 15.0% or more 4.0% or more Crude fiber
1.0% or less 4.0% or less Crude 10.
0% or less 7.0% or less Calcium 0.6% or more 0.6% or more Phosphorus 0.4
% or more 0.4% or more - Hi DCP 2
5.5% or more 19.0% or more DN 98.
In the group where feed additive A was administered containing Miyajin bacteria of 0% or more and 77.0% or more,
Serum vitamin E at the time of introduction (10 days old) was 113 μg.
/dλ, but at 60 days old it was 156 μo/di, 9
226 μ at 0 day old! II/dffi increased, and in both cases, significant differences (P<0.05) were observed between the control group and the control group. On the other hand, in the groups treated with Feed Additive B, which does not contain Miyanobacterium, serum vitamin E tended to decrease with age, and only a slight upward trend was observed after 60 days of age. Ta.

Example 2 Example for chickens (chicks) Commercially available feed for young chicks (1k) having the feed ingredients shown in Table 4
per liter of C, Iostridium bu
A vitamin E absorption enhancer C containing 1Q (containing 107 pieces/g or more of tyricum Miyairi M (Feikoken Bibori No. 146)) was prepared.

In addition, commercially available feed for young chicks 1 having the feed ingredients shown in Table 4
per kg, 1 g of Miyanobacterium powder and vitamin E preparation (formerly α
-Tocopherol acetate 100III Q/(J
A vitamin E absorption enhancer was prepared containing >10.

Forty 2-day-old chicken chicks were divided into 4 groups of 10 chickens each, separated in cages in each test group, and tested until they were 4 weeks old with free feeding and drinking water. The test feeds were the above-mentioned commercially available feed for young chicks in the C agent control group, vitamin E absorption enhancer C in the C agent control group, and the above commercially available feed for young chicks in the D agent control group. Vitamin E preparation (containing d+-α-tocopherol acetate 10011g/g) 1 (I
In addition, in the group where drug D was administered, an absorption enhancer was given.

Blood was collected from each chick as a test specimen at the third withdrawal and the fourth week, and the serum vitamin E concentration was measured from the collected blood. The results are shown in Table 5 and Figure 2.

Table 4 Feed ingredients for young chicks Crude protein 23.04% Digestible protein 19.03% Crude fat 6.40% Crude fiber 2.91% Vitamin A 8000ItJ/kghi') Min D31600 I LJ/k (+vitamin E
8111 (1/kg-^-) Serum vitamin Ellr1 is 4 from 3 weeks old in all groups.
The values were lower at 3 and 4 weeks of age, and the lowest 1 was in the C formulation control group, which was fed only commercially available chick feed. The group treated with Formulation D, which was given the vitamin E absorption enhancer prepared by adding the vitamin E absorption enhancer, showed the highest level of resistance.

The C agent 1u group given Gotamin E absorption enhancer C prepared by adding Miyato bacteria to commercially available feed for young chicks had a higher value than the C agent control group, and the 4I In the age group, the C drug control group was 1.8a+g/dl, while the J drug administered group was 2.
．． It increased to 61 g/dffi, and a significant difference (Pro, 05) was observed between the C agent control group and the control group.

In addition, a similar trend was observed between the drug D administration group and the drug D control group, with 43! At 1 year old, the D agent control area was 2.50 m.
g/dl in the D drug administration area, 5°/410/
dffi increased, and there was a significant difference (P<
0.05) was observed.

Example 3 Example on humans As shown in Example 1 and Example 2, the vitamin E promoting effect of Miyajin bacteria was observed on cows and chickens (chicks), so we conducted a similar study on humans. I did it.

The test drug was Miyanobacterium preparation (Miyajinbacterial powder 30n+g/g
) and a commercially available vitamin E preparation (containing 50 mQ/tablet of old-α-tocopherol acetate) were used.

Vitamin E preparations and Miyajin bacteria preparations were administered to adult males (5 persons) according to the administration schedule shown in Table 6. Two tablets of vitamin E preparations a day, and two tablets of Miyanobacterium preparations a day.
Each dose was divided into two doses per day.

Table 6 Method of administration of test drug Administration period Test drug 1 to 30 days Vitamin E 31 to 60 days For each person who underwent the vitamin E uterine entry test, 7 days before administration, immediately before administration, at the completion of the vitamin E administration period, Blood was collected at the end of the vitamin E administration period, 7 days after the end of the j9 administration, and 14 days after the end of the administration, and the serum vitamin concentration (μO/d 1 >) was measured from the collected blood.The results are shown in Table 7. The measured values before and after administration in Table 7 are expressed as the average values of two measurements, 7 days before administration, immediately before administration, 7 days after the end of administration, and 14 days after the end of administration, respectively.

(Margins below) Serum vitamin E levels increase by 12-71% when taking vitamin E preparations, but when vitamin E preparations are taken at the same time as the Miyajin bacterium preparation, they are lower than when vitamin E preparations are taken alone. It can be seen that this has increased by 18 to 28%.

(Effects of the Invention) As described above, the present invention is a vitamin E absorption enhancer characterized by containing Clostridium butyricum Miyairi as an active ingredient. The absorption of E is promoted and its pharmacological effects can be enhanced.

In addition, since the absorption of vitamin E is promoted by using this principle, there is no need to administer large amounts of expensive vitamin E preparations as in the past in order to reach the desired intake, which is economically advantageous. .

Furthermore, by using the basic rules, even if vitamin E is administered orally as a solid form, it can be absorbed relatively well into the intestine, and it is especially useful for livestock and poultry to effectively ingest vitamin E. It is very advantageous because it can be simply blended into commonly consumed feed and administered orally, without requiring complicated methods such as giving the drug a drink as a liquid or parenterally administering it as an injection.

[Brief explanation of the drawing]

FIG. 1 is a graph showing changes in serum vitamin E concentration in rearing cows, and FIG. 2 is a graph showing changes in serum vitamin E concentration in chickens (chicks).

Claims (6)

[Claims] (1) A vitamin E absorption enhancer characterized by containing Clostridium butyricum Miyairi as an active ingredient. (2) The vitamin E absorption enhancer according to claim 1, which is administered to humans. (3) The vitamin E absorption enhancer according to claim 1, which is administered to livestock or poultry. (4) The vitamin E absorption enhancer according to any one of claims 1 to 3, wherein the Clostridium butyricum miyairi is a blend with a carrier. (5) The vitamin E absorption enhancer according to claim 4, wherein the carrier is feed for livestock or poultry. (6) The vitamin E absorption enhancer according to claim 4, wherein the carrier is lactose or starch.